Prupe.1G439300_v2.0.a1

carboxylesterase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0011899
Physical Location & Seq
Forward (+)
37366066 .. 37367407
1342 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G439300.1

Sequence Viewer

Length: 960 bp
ATGGCTTCAACCACATCACCAGAAGTCTCACTTGAAGTCTTGCCCTACCTGCGAGTGCTCAAAGACGGAACAATTGACAGAATCGCCGGAACCCAAGTCTTCCCGGCCGGCCTGGACCCTCAGACCGGAGTTTTATCCAAAGACATCGTCCTCATACCCGAAACCGGAGTCTCGGCCAGGCTCTACATACCCAACAACATCACCAAAACTGATCACAAGCTTCCCCTCATCGTTTACTTCCATGGCGGGGCCTTTTTCATATCCTCAGCCGCCGACCCGCTTTACCACAACAGCCTCAACATGCTTGTTGCTGAAGCAAAAGCCATCGCTGTTTCGGTAAATTACAGGTTGGCCCCTGAGCACCCCCTCCCTACTGCATATGAAGACTCTTGGGCTGCACTCAATTGGGTGTTTGGTGGTGGAGAAGATGGTGATTTGTGGGTTAAGGAGCATGTTGATTTTGGGCGGGTGTTCTTGGTTGGAGACAGTGCAGGGGCTAATATTGCACACCATTTGGCCTTACGGGTGAAGGCATCCGACGCGGATCCGAAGGTGAAGATTGCCGGGATTGGTTTGGTCCATCCTTATTTTTGGGGGAAGGAGCCAATTGGTGGAGAGGTGACAGATTTGGTGAGGAAGTCAATGGTGGACAAGTGGTGGCAGTTCGTTTGCCCGTCAGAGAAAGCGGGGGATGATCCGCTTATTAACCCCTTTGGGGATGGAGCCCCGAGTGTTGAGGGGTTGGCTTGTGGGAAAGTGCTTGTTCTTGTTGCGGGAAAAGACATATTGAGGGACAGAGGGAGGCTTTATTATGATGAACTGGTGAAGAGCAGTTGGAGAGGGAGGAAGGAGTATACTGAAACAGAAGGCGAGGACCATGTGTTTCACATCTTTAATCCCAATTGTGAAAAAGCTAAGAGCTTGATTAAGCATTTGGCTTCTTTCATAAACCAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

320

Amino Acids

34.97

Weight (kDa)

5.67

Isoelectric Point (pI)

25.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 57
AccB7I CCANNNNNTGG 1 cut(s) 611
AccI GTMKAC 1 cut(s) 854
AccII CGCG 1 cut(s) 542
AciI CCGC 8 cut(s) 246, 270, 278, 466, 542, 686, 698, 773
AclWI GGATC 3 cut(s) 539, 552, 689
AcoI YGGCCR 2 cut(s) 105, 174
AcuI CTGAAG 1 cut(s) 333
AfiI CCNNNNNNNGG 5 cut(s) 125, 164, 247, 611, 715
AgsI TTSAA 2 cut(s) 9, 35
AjnI CCWGG 2 cut(s) 111, 176
AluBI AGCT 3 cut(s) 220, 914, 921
AluI AGCT 3 cut(s) 220, 914, 921
Alw21I GWGCWC 2 cut(s) 60, 363
Alw26I GTCTC 3 cut(s) 31, 175, 477
AlwI GGATC 3 cut(s) 539, 552, 689
Ama87I CYCGRG 1 cut(s) 727
AoxI GGCC 6 cut(s) 105, 109, 174, 249, 351, 516
ApeKI GCWGC 1 cut(s) 395
ArsI GACNNNNNNTTYG 4 cut(s) 132, 153, 164, 185
AspS9I GGNCC 5 cut(s) 115, 249, 352, 577, 874
AsuC2I CCSGG 2 cut(s) 104, 565
AsuHPI GGTGA 8 cut(s) 9, 193, 443, 538, 565, 631, 643, 835
AvaI CYCGRG 1 cut(s) 727
AvaII GGWCC 3 cut(s) 115, 577, 874
BamHI GGATCC 1 cut(s) 544
BanII GRGCYC 1 cut(s) 727
BbsI GAAGAC 2 cut(s) 91, 390
Bbv12I GWGCWC 2 cut(s) 60, 363
BbvCI CCTCAGC 1 cut(s) 265
BbvI GCAGC 1 cut(s) 382
BccI CCATC 4 cut(s) 332, 422, 588, 713
BciT130I CCWGG 2 cut(s) 113, 178
BclI TGATCA 1 cut(s) 211
BcnI CCSGG 2 cut(s) 104, 565
BcoDI GTCTC 3 cut(s) 31, 175, 477
BfuAI ACCTGC 1 cut(s) 57
BisI GCNGC 2 cut(s) 270, 396
BlsI GCNGC 2 cut(s) 271, 397
Bme1390I CCNGG 4 cut(s) 104, 113, 178, 565
Bme18I GGWCC 3 cut(s) 115, 577, 874
BmeT110I CYCGRG 1 cut(s) 727
BmgT120I GGNCC 5 cut(s) 115, 249, 352, 577, 874
BmiI GGNNCC 7 cut(s) 91, 117, 250, 354, 546, 603, 724
BmrFI CCNGG 4 cut(s) 104, 113, 178, 565
BmsI GCATC 1 cut(s) 542
BpiI GAAGAC 2 cut(s) 91, 390
Bpu10I CCTNAGC 2 cut(s) 265, 357
BpuMI CCSGG 2 cut(s) 104, 565
BsaJI CCNNGG 1 cut(s) 241
BsaWI WCCGGW 2 cut(s) 125, 164
Bsc4I CCNNNNNNNGG 5 cut(s) 125, 164, 247, 611, 715
Bse118I RCCGGY 1 cut(s) 107
Bse1I ACTGG 1 cut(s) 825
BseBI CCWGG 2 cut(s) 113, 178
BseDI CCNNGG 1 cut(s) 241
BseGI GGATG 4 cut(s) 533, 580, 697, 724
BseLI CCNNNNNNNGG 5 cut(s) 125, 164, 247, 611, 715
BseMII CTCAG 3 cut(s) 134, 279, 348
BseNI ACTGG 1 cut(s) 825
BseX3I CGGCCG 1 cut(s) 105
BseXI GCAGC 1 cut(s) 382
BsgI GTGCAG 2 cut(s) 381, 510
Bsh1236I CGCG 1 cut(s) 542
Bsh1285I CGRYCG 1 cut(s) 108
BshFI GGCC 6 cut(s) 107, 111, 176, 251, 353, 518
BsiEI CGRYCG 1 cut(s) 108
BsiHKAI GWGCWC 2 cut(s) 60, 363
BsiHKCI CYCGRG 1 cut(s) 727
BsiSI CCGG 6 cut(s) 87, 104, 108, 126, 165, 564
BslFI GGGAC 1 cut(s) 806
BslI CCNNNNNNNGG 5 cut(s) 125, 164, 247, 611, 715
BsmAI GTCTC 3 cut(s) 31, 175, 477
BsmFI GGGAC 1 cut(s) 806
BsnI GGCC 6 cut(s) 107, 111, 176, 251, 353, 518
BsoBI CYCGRG 1 cut(s) 727
Bsp1286I GDGCHC 3 cut(s) 60, 363, 727
Bsp143I GATC 3 cut(s) 211, 544, 694
Bsp19I CCATGG 1 cut(s) 241
BspACI CCGC 8 cut(s) 246, 270, 278, 466, 542, 686, 698, 773
BspANI GGCC 6 cut(s) 107, 111, 176, 251, 353, 518
BspCNI CTCAG 3 cut(s) 133, 278, 349
BspFNI CGCG 1 cut(s) 542
BspLI GGNNCC 7 cut(s) 91, 117, 250, 354, 546, 603, 724
BspMI ACCTGC 1 cut(s) 57
BspPI GGATC 3 cut(s) 539, 552, 689
BspQI GCTCTTC 1 cut(s) 821
BsrFI RCCGGY 1 cut(s) 107
BsrI ACTGG 1 cut(s) 825
BssAI RCCGGY 1 cut(s) 107
BssECI CCNNGG 1 cut(s) 241
BssMI GATC 3 cut(s) 211, 544, 694
BssNAI GTATAC 1 cut(s) 855
BssT1I CCWWGG 1 cut(s) 241
Bst1107I GTATAC 1 cut(s) 855
Bst2UI CCWGG 2 cut(s) 113, 178
Bst4CI ACNGT 1 cut(s) 488
Bst6I CTCTTC 1 cut(s) 821
BstC8I GCNNGC 1 cut(s) 109
BstDEI CTNAG 4 cut(s) 120, 265, 357, 915
BstDSI CCRYGG 1 cut(s) 241
BstF5I GGATG 4 cut(s) 533, 580, 697, 724
BstFNI CGCG 1 cut(s) 542
BstKTI GATC 3 cut(s) 214, 547, 697
BstMAI GTCTC 3 cut(s) 31, 175, 477
BstMBI GATC 3 cut(s) 211, 544, 694
BstMCI CGRYCG 1 cut(s) 108
BstMWI GCNNNNNNNGC 3 cut(s) 49, 503, 539
BstNI CCWGG 2 cut(s) 113, 178
BstNSI RCATGY 2 cut(s) 304, 455
BstSCI CCNGG 4 cut(s) 102, 111, 176, 563
BstUI CGCG 1 cut(s) 542
BstV1I GCAGC 1 cut(s) 382
BstV2I GAAGAC 2 cut(s) 91, 390
BstX2I RGATCY 1 cut(s) 544
BstYI RGATCY 1 cut(s) 544
BstZ17I GTATAC 1 cut(s) 855
BstZI CGGCCG 1 cut(s) 105
BsuRI GGCC 6 cut(s) 107, 111, 176, 251, 353, 518
BtgI CCRYGG 1 cut(s) 241
BtgZI GCGATG 1 cut(s) 310
BtsCI GGATG 4 cut(s) 533, 580, 697, 724
BtsIMutI CAGTG 1 cut(s) 493
BveI ACCTGC 1 cut(s) 57
Cac8I GCNNGC 1 cut(s) 109
Cfr10I RCCGGY 1 cut(s) 107
Cfr13I GGNCC 5 cut(s) 115, 249, 352, 577, 874
CseI GACGC 1 cut(s) 548
CviAII CATG 4 cut(s) 242, 301, 452, 878
DdeI CTNAG 4 cut(s) 120, 265, 357, 915
DpnI GATC 3 cut(s) 213, 546, 696
DpnII GATC 3 cut(s) 211, 544, 694
EaeI YGGCCR 2 cut(s) 105, 174
EagI CGGCCG 1 cut(s) 105
Eam1104I CTCTTC 1 cut(s) 821
EarI CTCTTC 1 cut(s) 821
EclXI CGGCCG 1 cut(s) 105
Eco130I CCWWGG 1 cut(s) 241
Eco24I GRGCYC 1 cut(s) 727
Eco47I GGWCC 3 cut(s) 115, 577, 874
Eco52I CGGCCG 1 cut(s) 105
Eco57I CTGAAG 1 cut(s) 333
Eco88I CYCGRG 1 cut(s) 727
EcoO109I RGGNCCY 1 cut(s) 249
EcoRII CCWGG 2 cut(s) 111, 176
EcoT14I CCWWGG 1 cut(s) 241
EcoT38I GRGCYC 1 cut(s) 727
ErhI CCWWGG 1 cut(s) 241
FaeI CATG 4 cut(s) 245, 304, 455, 881
FalI AAGNNNNNCTT 2 cut(s) 15, 47
FaqI GGGAC 1 cut(s) 806
FatI CATG 4 cut(s) 241, 300, 451, 877
FauI CCCGC 5 cut(s) 239, 285, 459, 679, 766
FauNDI CATATG 1 cut(s) 379
FbaI TGATCA 1 cut(s) 211
FblI GTMKAC 1 cut(s) 854
Fnu4HI GCNGC 2 cut(s) 270, 396
FokI GGATG 4 cut(s) 520, 567, 704, 731
FriOI GRGCYC 1 cut(s) 727
FseI GGCCGGCC 1 cut(s) 111
Fsp4HI GCNGC 2 cut(s) 270, 396
GluI GCNGC 2 cut(s) 270, 396
HaeIII GGCC 6 cut(s) 107, 111, 176, 251, 353, 518
HapII CCGG 6 cut(s) 87, 104, 108, 126, 165, 564
HgaI GACGC 1 cut(s) 548
Hin1II CATG 4 cut(s) 245, 304, 455, 881
HindIII AAGCTT 1 cut(s) 218
HinfI GANTC 3 cut(s) 81, 168, 386
HpaII CCGG 6 cut(s) 87, 104, 108, 126, 165, 564
HphI GGTGA 8 cut(s) 9, 193, 443, 538, 565, 631, 643, 835
Hpy166II GTNNAC 3 cut(s) 235, 649, 855
Hpy188I TCNGA 4 cut(s) 123, 538, 549, 679
Hpy8I GTNNAC 3 cut(s) 235, 649, 855
Hpy99I CGWCG 1 cut(s) 542
HpyAV CCTTC 5 cut(s) 523, 544, 592, 841, 860
HpyCH4III ACNGT 1 cut(s) 488
HpyCH4V TGCA 4 cut(s) 377, 398, 491, 506
HpyF10VI GCNNNNNNNGC 3 cut(s) 49, 503, 539
HpyF3I CTNAG 4 cut(s) 120, 265, 357, 915
Hsp92II CATG 4 cut(s) 245, 304, 455, 881
KroI GCCGGC 1 cut(s) 107
KroNI GCCGGC 1 cut(s) 109
Ksp22I TGATCA 1 cut(s) 211
Kzo9I GATC 3 cut(s) 211, 544, 694
LguI GCTCTTC 1 cut(s) 821
LmnI GCTCC 3 cut(s) 448, 601, 722
Lsp1109I GCAGC 1 cut(s) 382
LweI GCATC 1 cut(s) 542
MaeIII GTNAC 1 cut(s) 619
MalI GATC 3 cut(s) 213, 546, 696
MboI GATC 3 cut(s) 211, 544, 694
MboII GAAGA 5 cut(s) 91, 395, 437, 568, 838
MfeI CAATTG 4 cut(s) 72, 403, 606, 901
MflI RGATCY 1 cut(s) 544
MhlI GDGCHC 3 cut(s) 60, 363, 727
MluCI AATT 5 cut(s) 72, 340, 403, 606, 901
MlyI GAGTC 2 cut(s) 177, 380
MmeI TCCRAC 3 cut(s) 460, 561, 815
MroNI GCCGGC 1 cut(s) 107
MseI TTAA 4 cut(s) 444, 705, 894, 927
MspI CCGG 6 cut(s) 87, 104, 108, 126, 165, 564
MspR9I CCNGG 4 cut(s) 104, 113, 178, 565
MunI CAATTG 4 cut(s) 72, 403, 606, 901
MvaI CCWGG 2 cut(s) 113, 178
MvnI CGCG 1 cut(s) 542
MwoI GCNNNNNNNGC 3 cut(s) 49, 503, 539
NaeI GCCGGC 1 cut(s) 109
NciI CCSGG 2 cut(s) 104, 565
NcoI CCATGG 1 cut(s) 241
NdeI CATATG 1 cut(s) 379
NdeII GATC 3 cut(s) 211, 544, 694
NgoMIV GCCGGC 1 cut(s) 107
NlaIII CATG 4 cut(s) 245, 304, 455, 881
NlaIV GGNNCC 7 cut(s) 91, 117, 250, 354, 546, 603, 724
NmeAIII GCCGAG 1 cut(s) 152
NmuCI GTSAC 1 cut(s) 619
NspI RCATGY 2 cut(s) 304, 455
PciSI GCTCTTC 1 cut(s) 821
PdiI GCCGGC 1 cut(s) 109
PfeI GAWTC 1 cut(s) 81
PflFI GACNNNGTC 1 cut(s) 146
PflMI CCANNNNNTGG 1 cut(s) 611
PkrI GCNGC 2 cut(s) 271, 397
PleI GAGTC 2 cut(s) 176, 380
PpsI GAGTC 2 cut(s) 176, 380
Psp6I CCWGG 2 cut(s) 111, 176
PspGI CCWGG 2 cut(s) 111, 176
PspN4I GGNNCC 7 cut(s) 91, 117, 250, 354, 546, 603, 724
PspPI GGNCC 5 cut(s) 115, 249, 352, 577, 874
PsuI RGATCY 1 cut(s) 544
PsyI GACNNNGTC 1 cut(s) 146
RigI GGCCGGCC 1 cut(s) 111
SapI GCTCTTC 1 cut(s) 821
SaqAI TTAA 4 cut(s) 444, 705, 894, 927
SatI GCNGC 2 cut(s) 270, 396
Sau3AI GATC 3 cut(s) 211, 544, 694
Sau96I GGNCC 5 cut(s) 115, 249, 352, 577, 874
SchI GAGTC 2 cut(s) 177, 380
ScrFI CCNGG 4 cut(s) 104, 113, 178, 565
SduI GDGCHC 3 cut(s) 60, 363, 727
SetI ASST 7 cut(s) 51, 222, 350, 555, 621, 916, 923
SfaNI GCATC 1 cut(s) 542
SinI GGWCC 3 cut(s) 115, 577, 874
Sse9I AATT 5 cut(s) 72, 340, 403, 606, 901
SsiI CCGC 8 cut(s) 246, 270, 278, 466, 542, 686, 698, 773
SspI AATATT 1 cut(s) 502
StyD4I CCNGG 4 cut(s) 102, 111, 176, 563
StyI CCWWGG 1 cut(s) 241
TaaI ACNGT 1 cut(s) 488
TasI AATT 5 cut(s) 72, 340, 403, 606, 901
TauI GCSGC 1 cut(s) 272
TfiI GAWTC 1 cut(s) 81
Tru1I TTAA 4 cut(s) 444, 705, 894, 927
Tru9I TTAA 4 cut(s) 444, 705, 894, 927
TscAI CASTG 1 cut(s) 493
TseFI GTSAC 1 cut(s) 619
TseI GCWGC 1 cut(s) 395
Tsp45I GTSAC 1 cut(s) 619
TspDTI ATGAA 4 cut(s) 247, 396, 831, 934
TspGWI ACGGA 1 cut(s) 81
TspRI CASTG 1 cut(s) 493
Tth111I GACNNNGTC 1 cut(s) 146
Van91I CCANNNNNTGG 1 cut(s) 611
VpaK11BI GGWCC 3 cut(s) 115, 577, 874
XceI RCATGY 2 cut(s) 304, 455
XmiI GTMKAC 1 cut(s) 854
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.