Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0009861

Physical Location & Seq

Genomic Coordinates Forward (+)

11051657 .. 11054890

Gene Structure 3234 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1120200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2133 bp

ATGGGGTGCGCCCAATCGAGAATCGACAACGAGGAATCTGTGTCGCGATGTAAGGAACGGAGAAACCTAATGAAAGAGGCGGTGGTGGCCCGGAACGCCTTCGCTTCCGGTCATTCCGGCTATGCCGTGTCCTTGAAGAACGCCGGCGCTGCGTTGAGTGATTATGGCCATGGAGAAACCCAAGTGACCCAAGAGATGGAAATTCTTCACTGCCAACACCGGCCGATGGACCCTGCATCTGAGCCGCCGCCCCATTTGGAGAATTTGAACCTTCCGCCGCCTCCGCCGCCTTTGCCCACTTTTACTCCGAGTCCGATCAAGCGGGCCATCAGCATGCCGGCGATGAGCGCTGAGGCTCGAAAAATGGGGGGGCGGAGAGTTGGGCTGGCCATTGCCGAGGAGGATGAGGAAGAAGAAGAAGACCATGAACATGGTGAGGATGAGAGCCATAAAGGATTTCAGAGGAGATCGAGAAATGGGGCTTCGGAGACGACGTCGTCGCCGCCGCCGAGGACGCCGGAGATGAAGGCGGTGCCGCCAATGCCGGAGTCGAAGGGCATGGCTTGGGACTATTTCTTTATGGTGGATAACATGCCGGGACCTTCTTTGAGTGAGAATGAAGAAGCTGAGGAATTTGGTGAAGATGAGAATATGGGGATTGGTGGCGGCGGCGGCGGTGATGTGGATGATGGGGTTGAACCTAAGACTCCGGAAAAAGTGGAGGATATTGAGGAGAAGCCGGAAGAGGAGACTCCGGTGAAGGTGGCAATTGAGCATTCGAAGACTGCACCACCGGAGTTCAGTAGGAGAGTAGCTAATGTGATTCCTGGTGTGACTTTGATGGACATATTGAATAAGATTGATGATCATTTCTTGAAGGCCTCCGAGAGTGCTCAGGAGGTTTCCAAGATGCTCGAGGCCACTCGGTTGCATTATCACTCGAATTTCGCAGATAATCGAGGACACATTGATCACTCTGCAAGGGTCATGCGTGTTATTACATGGAATAGGTCGTTCAGAGGCATACCAAATGGTGACGGGAAGGATGAAAACTCAGAAGATTATGAAACTCATGCCACTGTTTTGGATAAGATGTTGGCATGGGAAAAGAAACTTTATGATGAAGTGAAGCAAGGGGAGCTTATGAAGGTTGAGTATCAGAGAAAGGTTGCTCTGCTGAATAAGCAGAAGAAACGCAGCGCTAGTGCTGAAACCTTAGAAAAAACAAAAGCAGCTGTAAGTCATTTGCATACAAGATACATTGTTGACATGCAGTCCATGGATTCTACAGTTTCGGAAGTAAATCAATTACGGGATGAGCAGCTGTACCCTAAGCTTGTTTCTCTAGCTGATGGGATGGCAAGTATGTGGGAGAACATGTGCACACATCACGACAGCCAGCTGAAGATTGTTACAGACCTAAAGTCCCTCGACATTGGCCATACTCCAATGGAAACAACCAAGCACCACCATGCCCGCACTGTCCAACTTTGGAACGTTCTCCGAGAATGGCATTCTCACTTCGAAAATCTCGTGACTCATCAAAAACAATACATCCAAGCTCTTAATAGCTGGTTGAAGCTTAATCTCATTCCCATTGAAAGCAGCTTAAAAGAGAAAATCTCATCCCCGCCGAGAGTTCAGCATCCGCCAATCCAAGCTCTCCTCCATTCATGGCACGATTTTCTTGAAAAGCTTCCTGATGAACTCGCAAAATCTGCAATATCATCCTTTTCGGCTGTGATAAAGACCATAATACTTCATCAGGAGGAGGAGATGAAGCTAAAGGAGAAGTTTGAGGAAACAAGGAAGGAGTATCTGCGTAAAAATCAGGCATTCGAGGACTGGTATCAAAAGTACTCGCAGAGACACGCGGAAATGGATGAGGAGAGAGGTGAAGATGCAGTTCCCAAGGATCCCGTCTCAGAGAAGAAATTTATTGTTGATAGCTTGAAGAAGAGACTGGAAGAGGAATGTGAAGCTCACCAGAGACACTGTATACAGGTGAGGGAGAAGTCACTCGGGAGTCTCAAAACTCGCCTGCCTGAGATCTTCCGCGCCATGACAGACTACGCTCGTGCATGTTCCGAGGCTTATGGAAAACTGAGGTCCATTAGAGAGACACAAGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF630 DUF632

Protein Analysis

711

Amino Acids

80.8

Weight (kDa)

5.69

Isoelectric Point (pI)

54.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF630	PF04783	1 - 59	3.8e-22	Protein of unknown function (DUF630)
DUF632	PF04782	280 - 583	2.5e-107	Protein of unknown function (DUF632)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009861)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G25590
fragaria_vesca	FvH4_2g36270
malus_domestica	MD08G1120200.v1.1 MD15G1099400.v1.1
prunus_persica	Prupe.1G454100_v2.0.a1
pyrus_communis	pycom15g09140
rosa_chinensis	RchiOBHm_Chr6g0305621
rosa_laevigata	RLG00000010872 RLG00000018217
rosa_multiflora	Rmu_co8402805.1_g000001 Rmu_co8491339.1_g000001 Rmu_sc0001809.1_g000015
rosa_roxburghii	Rroxscaffold_7G00162520
rosa_rugosa	Rorug06G0342200
rosa_samantha	Rh2AG452300 Rh4BG182200 Rh6AG454100 Rh6BG447400 Rh6CG467800 Rh6DG454400
rosa_wichuraiana	Rw6G039590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	496
AatII	GACGTC	1 cut(s)	497
AccB1I	GGYRCC	1 cut(s)	532
AccB7I	CCANNNNNTGG	1 cut(s)	196
AccI	GTMKAC	1 cut(s)	1999
AccII	CGCG	3 cut(s)	46, 1874, 2058
AccIII	TCCGGA	1 cut(s)	709
AclI	AACGTT	1 cut(s)	1497
AclWI	GGATC	2 cut(s)	1910, 1923
AcoI	YGGCCR	4 cut(s)	166, 221, 387, 1438
AcsI	RAATTY	5 cut(s)	201, 262, 632, 943, 1934
AcuI	CTGAAG	1 cut(s)	1424
AcyI	GRCGYC	2 cut(s)	494, 515
AfaI	GTAC	2 cut(s)	1328, 1859
AfeI	AGCGCT	2 cut(s)	349, 1201
AfiI	CCNNNNNNNGG	2 cut(s)	196, 226
AflIII	ACRYGT	1 cut(s)	1377
AgsI	TTSAA	9 cut(s)	136, 268, 698, 853, 877, 1579, 1601, 1691, 1954
AhdI	GACNNNNNGTC	2 cut(s)	1273, 1423
AjnI	CCWGG	1 cut(s)	826
Alw21I	GWGCWC	2 cut(s)	895, 1385
Alw26I	GTCTC	8 cut(s)	482, 743, 1861, 1927, 1954, 1984, 2033, 2114
Alw44I	GTGCAC	1 cut(s)	1381
AlwI	GGATC	2 cut(s)	1910, 1923
Ama87I	CYCGRG	2 cut(s)	914, 2021
Aor13HI	TCCGGA	1 cut(s)	709
Aor51HI	AGCGCT	2 cut(s)	349, 1201
AoxI	GGCC	8 cut(s)	87, 166, 221, 324, 387, 879, 918, 1438
ApaLI	GTGCAC	1 cut(s)	1381
ApeKI	GCWGC	5 cut(s)	149, 1197, 1232, 1321, 1605
ApoI	RAATTY	5 cut(s)	201, 262, 632, 943, 1934
Asp700I	GAANNNNTTC	3 cut(s)	98, 204, 1695
AspLEI	GCGC	5 cut(s)	11, 149, 350, 1202, 2060
AspS9I	GGNCC	5 cut(s)	88, 229, 324, 599, 2109
AsuC2I	CCSGG	2 cut(s)	91, 597
AsuHPI	GGTGA	8 cut(s)	446, 650, 689, 769, 1046, 1907, 1976, 2017
AsuII	TTCGAA	2 cut(s)	779, 1524
AvaI	CYCGRG	2 cut(s)	914, 2021
AvaII	GGWCC	3 cut(s)	229, 599, 2109
BaeGI	GKGCMC	1 cut(s)	1385
BalI	TGGCCA	3 cut(s)	168, 389, 1440
BamHI	GGATCC	1 cut(s)	1915
BanI	GGYRCC	1 cut(s)	532
BauI	CACGAG	2 cut(s)	1532, 2076
BbsI	GAAGAC	2 cut(s)	426, 788
Bbv12I	GWGCWC	2 cut(s)	895, 1385
BbvCI	CCTCAGC	2 cut(s)	351, 627
BbvI	GCAGC	5 cut(s)	136, 1209, 1244, 1333, 1617
BccI	CCATC	7 cut(s)	190, 220, 335, 683, 835, 1346, 1351
BceAI	ACGGC	1 cut(s)	110
BciT130I	CCWGG	1 cut(s)	828
BclI	TGATCA	2 cut(s)	865, 970
BcnI	CCSGG	2 cut(s)	91, 597
BcoDI	GTCTC	8 cut(s)	482, 743, 1861, 1927, 1954, 1984, 2033, 2114
BfaI	CTAG	2 cut(s)	1203, 1346
BfmI	CTRYAG	1 cut(s)	1287
BfoI	RGCGCY	3 cut(s)	150, 351, 1203
BglII	AGATCT	1 cut(s)	2049
BmcAI	AGTACT	1 cut(s)	1859
Bme1390I	CCNGG	3 cut(s)	91, 597, 828
Bme18I	GGWCC	3 cut(s)	229, 599, 2109
BmeRI	GACNNNNNGTC	2 cut(s)	1273, 1423
BmeT110I	CYCGRG	2 cut(s)	914, 2021
BmgT120I	GGNCC	5 cut(s)	88, 229, 324, 599, 2109
BmiI	GGNNCC	4 cut(s)	231, 534, 600, 1917
BmrFI	CCNGG	3 cut(s)	91, 597, 828
BmsI	GCATC	4 cut(s)	245, 900, 1654, 1891
BpiI	GAAGAC	2 cut(s)	426, 788
BplI	GAGNNNNNCTC	2 cut(s)	1607, 1639
Bpu10I	CCTNAGC	4 cut(s)	351, 627, 894, 1332
Bpu14I	TTCGAA	2 cut(s)	779, 1524
BpuMI	CCSGG	2 cut(s)	91, 597
BsaHI	GRCGYC	2 cut(s)	494, 515
BsaJI	CCNNGG	6 cut(s)	169, 396, 509, 1278, 1911, 2088
BsaWI	WCCGGW	4 cut(s)	107, 709, 754, 793
BsaXI	ACNNNNNCTCC	6 cut(s)	289, 319, 479, 509, 1364, 1394
Bsc4I	CCNNNNNNNGG	2 cut(s)	196, 226
Bse118I	RCCGGY	3 cut(s)	143, 219, 337
Bse1I	ACTGG	2 cut(s)	1850, 1968
Bse3DI	GCAATG	1 cut(s)	390
BseAI	TCCGGA	1 cut(s)	709
BseBI	CCWGG	1 cut(s)	828
BseDI	CCNNGG	6 cut(s)	169, 396, 509, 1278, 1911, 2088
BseLI	CCNNNNNNNGG	2 cut(s)	196, 226
BseMI	GCAATG	1 cut(s)	390
BseMII	CTCAG	8 cut(s)	231, 342, 618, 908, 1068, 1938, 2037, 2096
BseNI	ACTGG	2 cut(s)	1850, 1968
BseRI	GAGGAG	8 cut(s)	413, 478, 746, 761, 1655, 1784, 1787, 1901
BseSI	GKGCMC	1 cut(s)	1385
BseX3I	CGGCCG	1 cut(s)	221
BseXI	GCAGC	5 cut(s)	136, 1209, 1244, 1333, 1617
BsgI	GTGCAG	1 cut(s)	771
Bsh1236I	CGCG	3 cut(s)	46, 1874, 2058
Bsh1285I	CGRYCG	1 cut(s)	224
BshFI	GGCC	8 cut(s)	89, 168, 223, 326, 389, 881, 920, 1440
BshNI	GGYRCC	1 cut(s)	532
BsiEI	CGRYCG	1 cut(s)	224
BsiHKAI	GWGCWC	2 cut(s)	895, 1385
BsiHKCI	CYCGRG	2 cut(s)	914, 2021
BslFI	GGGAC	3 cut(s)	581, 612, 1411
BslI	CCNNNNNNNGG	2 cut(s)	196, 226
BsmAI	GTCTC	8 cut(s)	482, 743, 1861, 1927, 1954, 1984, 2033, 2114
BsmBI	CGTCTC	2 cut(s)	482, 1927
BsmFI	GGGAC	3 cut(s)	581, 612, 1411
BsmI	GAATGC	3 cut(s)	775, 1513, 1835
BsnI	GGCC	8 cut(s)	89, 168, 223, 326, 389, 881, 920, 1440
BsoBI	CYCGRG	2 cut(s)	914, 2021
Bsp119I	TTCGAA	2 cut(s)	779, 1524
Bsp1286I	GDGCHC	2 cut(s)	895, 1385
Bsp13I	TCCGGA	1 cut(s)	709
Bsp143I	GATC	6 cut(s)	315, 467, 865, 970, 1915, 2049
Bsp19I	CCATGG	2 cut(s)	169, 1278
Bsp68I	TCGCGA	1 cut(s)	46
BspANI	GGCC	8 cut(s)	89, 168, 223, 326, 389, 881, 920, 1440
BspCNI	CTCAG	8 cut(s)	232, 343, 619, 907, 1067, 1937, 2038, 2097
BspEI	TCCGGA	1 cut(s)	709
BspFNI	CGCG	3 cut(s)	46, 1874, 2058
BspLI	GGNNCC	4 cut(s)	231, 534, 600, 1917
BspPI	GGATC	2 cut(s)	1910, 1923
BspT104I	TTCGAA	2 cut(s)	779, 1524
BspT107I	GGYRCC	1 cut(s)	532
BsrDI	GCAATG	1 cut(s)	390
BsrFI	RCCGGY	3 cut(s)	143, 219, 337
BsrI	ACTGG	2 cut(s)	1850, 1968
BssAI	RCCGGY	3 cut(s)	143, 219, 337
BssECI	CCNNGG	6 cut(s)	169, 396, 509, 1278, 1911, 2088
BssMI	GATC	6 cut(s)	315, 467, 865, 970, 1915, 2049
BssNAI	GTATAC	1 cut(s)	2000
BssNI	GRCGYC	2 cut(s)	494, 515
BssSI	CACGAG	2 cut(s)	1532, 2076
BssT1I	CCWWGG	3 cut(s)	169, 1278, 1911
Bst1107I	GTATAC	1 cut(s)	2000
Bst2BI	CACGAG	2 cut(s)	1532, 2076
Bst2UI	CCWGG	1 cut(s)	828
Bst4CI	ACNGT	4 cut(s)	1081, 1291, 1483, 1997
Bst6I	CTCTTC	3 cut(s)	738, 1952, 1962
BstACI	GRCGYC	2 cut(s)	494, 515
BstAPI	GCANNNNNTGC	1 cut(s)	1718
BstBI	TTCGAA	2 cut(s)	779, 1524
BstC8I	GCNNGC	8 cut(s)	145, 324, 335, 339, 387, 1400, 1477, 2042
BstDSI	CCRYGG	2 cut(s)	169, 1278
BstFNI	CGCG	3 cut(s)	46, 1874, 2058
BstH2I	RGCGCY	3 cut(s)	150, 351, 1203
BstHHI	GCGC	5 cut(s)	11, 149, 350, 1202, 2060
BstKTI	GATC	6 cut(s)	318, 470, 868, 973, 1918, 2052
BstMAI	GTCTC	8 cut(s)	482, 743, 1861, 1927, 1954, 1984, 2033, 2114
BstMBI	GATC	6 cut(s)	315, 467, 865, 970, 1915, 2049
BstMCI	CGRYCG	1 cut(s)	224
BstNI	CCWGG	1 cut(s)	828
BstNSI	RCATGY	5 cut(s)	337, 595, 1273, 1381, 2085
BstSCI	CCNGG	3 cut(s)	89, 595, 826
BstSFI	CTRYAG	1 cut(s)	1287
BstSLI	GKGCMC	1 cut(s)	1385
BstUI	CGCG	3 cut(s)	46, 1874, 2058
BstV1I	GCAGC	5 cut(s)	136, 1209, 1244, 1333, 1617
BstV2I	GAAGAC	2 cut(s)	426, 788
BstX2I	RGATCY	2 cut(s)	1915, 2049
BstXI	CCANNNNNNTGG	2 cut(s)	431, 1084
BstYI	RGATCY	2 cut(s)	1915, 2049
BstZ17I	GTATAC	1 cut(s)	2000
BstZI	CGGCCG	1 cut(s)	221
BsuRI	GGCC	8 cut(s)	89, 168, 223, 326, 389, 881, 920, 1440
BtgI	CCRYGG	2 cut(s)	169, 1278
BtgZI	GCGATG	2 cut(s)	61, 356
BtsI	GCAGTG	1 cut(s)	208
BtsIMutI	CAGTG	4 cut(s)	208, 1077, 1479, 1993
BtuMI	TCGCGA	1 cut(s)	46
Cac8I	GCNNGC	8 cut(s)	145, 324, 335, 339, 387, 1400, 1477, 2042
CfoI	GCGC	5 cut(s)	11, 149, 350, 1202, 2060
Cfr10I	RCCGGY	3 cut(s)	143, 219, 337
Cfr13I	GGNCC	5 cut(s)	88, 229, 324, 599, 2109
CseI	GACGC	1 cut(s)	523
Csp6I	GTAC	2 cut(s)	1327, 1858
CviQI	GTAC	2 cut(s)	1327, 1858
DpnI	GATC	6 cut(s)	317, 469, 867, 972, 1917, 2051
DpnII	GATC	6 cut(s)	315, 467, 865, 970, 1915, 2049
DrdI	GACNNNNNNGTC	1 cut(s)	496
DriI	GACNNNNNGTC	2 cut(s)	1273, 1423
DseDI	GACNNNNNNGTC	1 cut(s)	496
EaeI	YGGCCR	4 cut(s)	166, 221, 387, 1438
EagI	CGGCCG	1 cut(s)	221
Eam1104I	CTCTTC	3 cut(s)	738, 1952, 1962
Eam1105I	GACNNNNNGTC	2 cut(s)	1273, 1423
EarI	CTCTTC	3 cut(s)	738, 1952, 1962
EciI	GGCGGA	4 cut(s)	264, 273, 388, 1638
EclXI	CGGCCG	1 cut(s)	221
Eco130I	CCWWGG	3 cut(s)	169, 1278, 1911
Eco147I	AGGCCT	1 cut(s)	881
Eco47I	GGWCC	3 cut(s)	229, 599, 2109
Eco47III	AGCGCT	2 cut(s)	349, 1201
Eco52I	CGGCCG	1 cut(s)	221
Eco57I	CTGAAG	1 cut(s)	1424
Eco88I	CYCGRG	2 cut(s)	914, 2021
EcoO109I	RGGNCCY	1 cut(s)	599
EcoRII	CCWGG	1 cut(s)	826
EcoT14I	CCWWGG	3 cut(s)	169, 1278, 1911
ErhI	CCWWGG	3 cut(s)	169, 1278, 1911
Esp3I	CGTCTC	2 cut(s)	482, 1927
FalI	AAGNNNNNCTT	2 cut(s)	1125, 1157
FaqI	GGGAC	3 cut(s)	581, 612, 1411
FauI	CCCGC	3 cut(s)	315, 1484, 1638
FbaI	TGATCA	2 cut(s)	865, 970
FblI	GTMKAC	1 cut(s)	1999
FspBI	CTAG	2 cut(s)	1203, 1346
GlaI	GCGC	5 cut(s)	10, 148, 349, 1201, 2059
HaeII	RGCGCY	3 cut(s)	150, 351, 1203
HaeIII	GGCC	8 cut(s)	89, 168, 223, 326, 389, 881, 920, 1440
HgaI	GACGC	1 cut(s)	523
HhaI	GCGC	5 cut(s)	11, 149, 350, 1202, 2060
Hin1I	GRCGYC	2 cut(s)	494, 515
Hin6I	GCGC	5 cut(s)	9, 147, 348, 1200, 2058
HinP1I	GCGC	5 cut(s)	9, 147, 348, 1200, 2058
HincII	GTYRAC	1 cut(s)	1267
HindII	GTYRAC	1 cut(s)	1267
HindIII	AAGCTT	3 cut(s)	1334, 1580, 1694
HphI	GGTGA	8 cut(s)	446, 650, 689, 769, 1046, 1907, 1976, 2017
Hpy166II	GTNNAC	3 cut(s)	1267, 1383, 2000
Hpy8I	GTNNAC	3 cut(s)	1267, 1383, 2000
Hpy99I	CGWCG	3 cut(s)	496, 499, 502
HpyCH4III	ACNGT	4 cut(s)	1081, 1291, 1483, 1997
HpyCH4IV	ACGT	2 cut(s)	494, 1497
HpySE526I	ACGT	2 cut(s)	494, 1497
Hsp92I	GRCGYC	2 cut(s)	494, 515
HspAI	GCGC	5 cut(s)	9, 147, 348, 1200, 2058
Kpn2I	TCCGGA	1 cut(s)	709
KroI	GCCGGC	2 cut(s)	143, 337
KroNI	GCCGGC	2 cut(s)	145, 339
Ksp22I	TGATCA	2 cut(s)	865, 970
Kzo9I	GATC	6 cut(s)	315, 467, 865, 970, 1915, 2049
LmnI	GCTCC	1 cut(s)	1138
Lsp1109I	GCAGC	5 cut(s)	136, 1209, 1244, 1333, 1617
LweI	GCATC	4 cut(s)	245, 900, 1654, 1891
MaeI	CTAG	2 cut(s)	1203, 1346
MaeII	ACGT	2 cut(s)	494, 1497
MaeIII	GTNAC	6 cut(s)	184, 832, 1034, 1411, 1534, 2016
MalI	GATC	6 cut(s)	317, 469, 867, 972, 1917, 2051
MboI	GATC	6 cut(s)	315, 467, 865, 970, 1915, 2049
MfeI	CAATTG	1 cut(s)	768
MflI	RGATCY	2 cut(s)	1915, 2049
MhlI	GDGCHC	2 cut(s)	895, 1385
MlsI	TGGCCA	3 cut(s)	168, 389, 1440
MluCI	AATT	7 cut(s)	201, 262, 632, 768, 943, 1307, 1934
MluNI	TGGCCA	3 cut(s)	168, 389, 1440
MlyI	GAGTC	6 cut(s)	319, 557, 700, 745, 1531, 2035
MmeI	TCCRAC	1 cut(s)	1510
Mox20I	TGGCCA	3 cut(s)	168, 389, 1440
MreI	CGCCGGCG	1 cut(s)	143
MroI	TCCGGA	1 cut(s)	709
MroNI	GCCGGC	2 cut(s)	143, 337
MroXI	GAANNNNTTC	3 cut(s)	98, 204, 1695
MscI	TGGCCA	3 cut(s)	168, 389, 1440
MseI	TTAA	3 cut(s)	1566, 1584, 1610
MslI	CAYNNNNRTG	4 cut(s)	332, 429, 1470, 2128
Msp20I	TGGCCA	3 cut(s)	168, 389, 1440
MspA1I	CMGCKG	3 cut(s)	1235, 1324, 1402
MspR9I	CCNGG	3 cut(s)	91, 597, 828
MunI	CAATTG	1 cut(s)	768
Mva1269I	GAATGC	3 cut(s)	775, 1513, 1835
MvaI	CCWGG	1 cut(s)	828
MvnI	CGCG	3 cut(s)	46, 1874, 2058
NaeI	GCCGGC	2 cut(s)	145, 339
NciI	CCSGG	2 cut(s)	91, 597
NcoI	CCATGG	2 cut(s)	169, 1278
NdeII	GATC	6 cut(s)	315, 467, 865, 970, 1915, 2049
NgoMIV	GCCGGC	2 cut(s)	143, 337
NlaIV	GGNNCC	4 cut(s)	231, 534, 600, 1917
NmeAIII	GCCGAG	3 cut(s)	421, 534, 1659
NmuCI	GTSAC	5 cut(s)	184, 832, 1034, 1534, 2016
NruI	TCGCGA	1 cut(s)	46
NspI	RCATGY	5 cut(s)	337, 595, 1273, 1381, 2085
NspV	TTCGAA	2 cut(s)	779, 1524
PaeI	GCATGC	1 cut(s)	337
PaeR7I	CTCGAG	1 cut(s)	914
PceI	AGGCCT	1 cut(s)	881
PciI	ACATGT	1 cut(s)	1377
PcsI	WCGNNNNNNNCGW	3 cut(s)	491, 506, 1530
PctI	GAATGC	3 cut(s)	775, 1513, 1835
PdiI	GCCGGC	2 cut(s)	145, 339
PdmI	GAANNNNTTC	3 cut(s)	98, 204, 1695
PfeI	GAWTC	4 cut(s)	21, 35, 823, 1283
PflFI	GACNNNGTC	2 cut(s)	493, 496
PflMI	CCANNNNNTGG	1 cut(s)	196
PleI	GAGTC	6 cut(s)	318, 556, 700, 745, 1531, 2034
PpsI	GAGTC	6 cut(s)	318, 556, 700, 745, 1531, 2034
PpuMI	RGGWCCY	1 cut(s)	599
PscI	ACATGT	1 cut(s)	1377
Psp1406I	AACGTT	1 cut(s)	1497
Psp5II	RGGWCCY	1 cut(s)	599
Psp6I	CCWGG	1 cut(s)	826
PspGI	CCWGG	1 cut(s)	826
PspN4I	GGNNCC	4 cut(s)	231, 534, 600, 1917
PspPI	GGNCC	5 cut(s)	88, 229, 324, 599, 2109
PspPPI	RGGWCCY	1 cut(s)	599
PspXI	VCTCGAGB	1 cut(s)	914
PsuI	RGATCY	2 cut(s)	1915, 2049
PsyI	GACNNNGTC	2 cut(s)	493, 496
PvuII	CAGCTG	3 cut(s)	1235, 1324, 1402
RruI	TCGCGA	1 cut(s)	46
RsaI	GTAC	2 cut(s)	1328, 1859
RsaNI	GTAC	2 cut(s)	1327, 1858
RseI	CAYNNNNRTG	4 cut(s)	332, 429, 1470, 2128
SaqAI	TTAA	3 cut(s)	1566, 1584, 1610
Sau3AI	GATC	6 cut(s)	315, 467, 865, 970, 1915, 2049
Sau96I	GGNCC	5 cut(s)	88, 229, 324, 599, 2109
ScaI	AGTACT	1 cut(s)	1859
SchI	GAGTC	6 cut(s)	319, 557, 700, 745, 1531, 2035
ScrFI	CCNGG	3 cut(s)	91, 597, 828
SduI	GDGCHC	2 cut(s)	895, 1385
SfaNI	GCATC	4 cut(s)	245, 900, 1654, 1891
SfcI	CTRYAG	1 cut(s)	1287
Sfr274I	CTCGAG	1 cut(s)	914
SfuI	TTCGAA	2 cut(s)	779, 1524
SgrAI	CRCCGGYG	1 cut(s)	143
SinI	GGWCC	3 cut(s)	229, 599, 2109
SlaI	CTCGAG	1 cut(s)	914
SmiMI	CAYNNNNRTG	4 cut(s)	332, 429, 1470, 2128
SmlI	CTYRAG	1 cut(s)	914
SmoI	CTYRAG	1 cut(s)	914
SphI	GCATGC	1 cut(s)	337
Sse9I	AATT	7 cut(s)	201, 262, 632, 768, 943, 1307, 1934
SseBI	AGGCCT	1 cut(s)	881
SspMI	CTAG	2 cut(s)	1203, 1346
StuI	AGGCCT	1 cut(s)	881
StyD4I	CCNGG	3 cut(s)	89, 595, 826
StyI	CCWWGG	3 cut(s)	169, 1278, 1911
TaaI	ACNGT	4 cut(s)	1081, 1291, 1483, 1997
TaiI	ACGT	2 cut(s)	497, 1500
TasI	AATT	7 cut(s)	201, 262, 632, 768, 943, 1307, 1934
TatI	WGTACW	1 cut(s)	1857
TfiI	GAWTC	4 cut(s)	21, 35, 823, 1283
Tru1I	TTAA	3 cut(s)	1566, 1584, 1610
Tru9I	TTAA	3 cut(s)	1566, 1584, 1610
TscAI	CASTG	4 cut(s)	215, 1084, 1486, 2000
TseFI	GTSAC	5 cut(s)	184, 832, 1034, 1534, 2016
TseI	GCWGC	5 cut(s)	149, 1197, 1232, 1321, 1605
Tsp45I	GTSAC	5 cut(s)	184, 832, 1034, 1534, 2016
TspGWI	ACGGA	1 cut(s)	73
TspRI	CASTG	4 cut(s)	215, 1084, 1486, 2000
Tth111I	GACNNNGTC	2 cut(s)	493, 496
Van91I	CCANNNNNTGG	1 cut(s)	196
VneI	GTGCAC	1 cut(s)	1381
VpaK11BI	GGWCC	3 cut(s)	229, 599, 2109
XapI	RAATTY	5 cut(s)	201, 262, 632, 943, 1934
XceI	RCATGY	5 cut(s)	337, 595, 1273, 1381, 2085
XhoI	CTCGAG	1 cut(s)	914
XmiI	GTMKAC	1 cut(s)	1999
XmnI	GAANNNNTTC	3 cut(s)	98, 204, 1695
XspI	CTAG	2 cut(s)	1203, 1346
ZraI	GACGTC	1 cut(s)	495
ZrmI	AGTACT	1 cut(s)	1859

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.