Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6B

Orthogroup ID

OG0009861

Physical Location & Seq

Genomic Coordinates Forward (+)

66281895 .. 66286413

Gene Structure 4519 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6BG447400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2160 bp

ATGGGGTGCGCGCAATCGAGAATCGACAATGAGGAATCGGTGTCGAGATGCAAGGAGCGGAGGAACCTGATGAGAGAAGCGGTGGTGGCCAGAAACGCCTTCGCGGCCGGCCATTCCGGCTACGTCATGGCCTTGAAGAACACCGGCGCCGCCTTAAGCGATTACGGCCACGGAGAATCACAGCAGACACAAGAAGAGCTACTCCTCCATCACGACCAGCGGCCACTCGACCCCACCGCAACCGAGCCGCCGCCTCCTCCTCCTCCGCCGCTCGAACACCTCCCTCCGCCGCCGCCGCCTCTGCCGACTTTCACTACTCCCAGCCCGCCGATCAAGCGCGCCACCAGCCTGCCGACGGAGGTCCGGAAAACGGGGATGCAGGGGGCTAGGTTGCCCATTGCTGAGGAGGACGAGGACGAGGACGAGGAAGAAGAGGACCATGAGGAAGAAAAAGGGTTTCGCCGGAGATCGAGAAAGGAAATGGGCGCCGCGGCGGCGGCGGCGACGGGGTCCTCATCGCCGCAGACGCCGGAGATGAAGGCGGTGCCGCCGCCGTTGCCGGAGTCCAAGGGTGCGGCTTGGGACTATTTCTTCATGGTGGATAACATGCCGGGGCACAGCTTGAGTGAGAATGAAGGTGAAGAGTTTGGTGAAGATGAGAATGTGAATGATTTGGGTGATGGGGATGGTGATGAGCCCAAGACCCCGGAGAAGGCGGAGGAGATTCAGGTGAAGCCGGAGGAGATTCCGGTGTCTCCAGTGACTCCGGCGACAATTGAGCATTCGAAGACAGCCCCACCGGATTTTCAGAGGATGGTGATGCCCGGTTTTACTTTGATGGAAATATTGAATCAGATTGATGATCATTTCTTAAAGGCCTCGGAGAGTGCTCAGGAGGTTTCCAAGATGCTTGAGGCCACTCGATTGCATTATCACTCGAATTTTGCTGATAATCGAGGACACATTGATCATTCTGCACGAGTCATGCGTGTTATTACTTGGAATAAATCCTTCAGAGGCTTACCAAATGGTGATGGAGCAAAGGAGGAAGATTCAGATGATTATGAAACTCATGCCACTGTTTTGGATAAGTTGTTAGCATGGGAGAAGAAACTTTATGATGAAGTGAAGCAAGGGGAGCTTATGAAGGTTGAGTATCAGAGAAAGGTTGCTCTACTAAACAAGCAGAAAAAACGCAATGCTAGTGCTGAAACCTTGGAAAAAACGAAAGCAGCAGTAAGTCACTTGCATACAAGATATATTGTTGACATGCAGTCCATGGACTCTACAGTTTCAGAAGTGAATCAGATACGGGATGAGCAGCTGTACCCTAAACTTGTTTCTCTAGCTGACGCGATGGGAAAAATGTGGGAAAATATGTGTGCGCATCATGACAGCCAGCTGAAGATTGTTATGGACCTGAAAGCCCTTGATATTGCCCATACTCCGATGGAAACGACCAGGCACCACCATGAACGCACTGAACAGCTATTTAACGTTGTCCAACATTGGCATTCTGAATTTGAAAAGCTTGTGATTCATCAGAAACAATACATCCAAGCATTGAATAGCTGGTTGAAGTTGAATCTGATTCCTATTGAAAGCAGCTTAAAAGAGAAAATCTCTTCCCCTCCAAGAGTTCAGCGTCCCCCAATTCAACCGCTTCTCCATGCATGGCATGATTTTCTTGAAAAGCTTCCTGATGAACTTGCAAAATCTGCAACATCATCCTTTGCGGCTGTGATAAAGACTATATTACTTCATCAGGAGGAAGAGATGAAGCTAAAGGAGCAATATGAGAATACAAGGAAGGACCACATGCGTAAAAATCAGGCATTTCAAGACTGGTACCAGAAGTATATGCAGCGTAAGGGGCCTGGTGAAGCAGATCAGGAGAAAAGTGAAGATGCGAACCACAAGGATCCTATTGCAGAGAGGCAATTTGCAGTTGAGAGCTTGAAGAAGAAAATGGAAGAGGAAGCTGAAGCTCACCAGAGACATTGTATCCAGGTCAGAGAGAAGTCACTCGGGAGTCTCAAAACTCGCTTGCCTGAGCTCTTCCGCGCAATGTCAGACTATGCTCACACATGTGCTGATGCTTACGGAAAACTTAGGTCCATCACAATGCCACAAGCTTCAAATGGTAACTCTCATGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF630 DUF632

Protein Analysis

719

Amino Acids

81.63

Weight (kDa)

5.61

Isoelectric Point (pI)

50.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF630	PF04783	1 - 59	9.7e-23	Protein of unknown function (DUF630)
DUF632	PF04782	279 - 583	1e-103	Protein of unknown function (DUF632)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009861)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G25590
fragaria_vesca	FvH4_2g36270
malus_domestica	MD08G1120200.v1.1 MD15G1099400.v1.1
prunus_persica	Prupe.1G454100_v2.0.a1
pyrus_communis	pycom15g09140
rosa_chinensis	RchiOBHm_Chr6g0305621
rosa_laevigata	RLG00000010872 RLG00000018217
rosa_multiflora	Rmu_co8402805.1_g000001 Rmu_co8491339.1_g000001 Rmu_sc0001809.1_g000015
rosa_roxburghii	Rroxscaffold_7G00162520
rosa_rugosa	Rorug06G0342200
rosa_samantha	Rh2AG452300 Rh4BG182200 Rh6AG454100 Rh6BG447400 Rh6CG467800 Rh6DG454400
rosa_wichuraiana	Rw6G039590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1386
Acc65I	GGTACC	1 cut(s)	1848
AccB1I	GGYRCC	5 cut(s)	146, 485, 544, 1464, 1848
AccBSI	CCGCTC	2 cut(s)	58, 271
AccII	CGCG	6 cut(s)	11, 104, 339, 491, 1355, 2064
AccIII	TCCGGA	1 cut(s)	363
AclI	AACGTT	1 cut(s)	1497
AclWI	GGATC	2 cut(s)	1916, 1929
AcoI	YGGCCR	5 cut(s)	87, 105, 109, 166, 221
AcsI	RAATTY	2 cut(s)	940, 1520
AcuI	CTGAAG	3 cut(s)	997, 1424, 2004
AcyI	GRCGYC	3 cut(s)	147, 486, 527
AfaI	GTAC	2 cut(s)	1328, 1850
AfiI	CCNNNNNNNGG	3 cut(s)	355, 370, 712
AflII	CTTAAG	1 cut(s)	154
AflIII	ACRYGT	1 cut(s)	2087
AhdI	GACNNNNNGTC	1 cut(s)	1273
AjnI	CCWGG	3 cut(s)	1460, 1876, 2007
AleI	CACNNNNGTG	1 cut(s)	2088
Alw21I	GWGCWC	2 cut(s)	892, 2058
Alw26I	GTCTC	3 cut(s)	759, 1990, 2039
AlwI	GGATC	2 cut(s)	1916, 1929
Ama87I	CYCGRG	1 cut(s)	2027
Aor13HI	TCCGGA	1 cut(s)	363
AoxI	GGCC	9 cut(s)	87, 105, 109, 129, 166, 221, 876, 915, 1874
ApeKI	GCWGC	4 cut(s)	1232, 1321, 1605, 1864
ApoI	RAATTY	2 cut(s)	940, 1520
Asp700I	GAANNNNTTC	1 cut(s)	1695
Asp718I	GGTACC	1 cut(s)	1848
AspLEI	GCGC	8 cut(s)	11, 13, 149, 339, 341, 488, 1387, 2066
AspS9I	GGNCC	7 cut(s)	361, 436, 510, 1417, 1813, 1874, 2115
AsuC2I	CCSGG	3 cut(s)	612, 707, 825
AsuHPI	GGTGA	9 cut(s)	650, 662, 689, 701, 742, 829, 1043, 1892, 1982
AsuII	TTCGAA	1 cut(s)	785
AvaI	CYCGRG	1 cut(s)	2027
AvaII	GGWCC	6 cut(s)	361, 436, 510, 1417, 1813, 2115
BaeGI	GKGCMC	1 cut(s)	618
BalI	TGGCCA	1 cut(s)	89
BamHI	GGATCC	1 cut(s)	1921
BanI	GGYRCC	5 cut(s)	146, 485, 544, 1464, 1848
BanII	GRGCYC	2 cut(s)	699, 2058
BauI	CACGAG	1 cut(s)	978
BbsI	GAAGAC	1 cut(s)	794
Bbv12I	GWGCWC	2 cut(s)	892, 2058
BbvCI	CCTCAGC	1 cut(s)	402
BbvI	GCAGC	4 cut(s)	1244, 1333, 1617, 1876
BccI	CCATC	9 cut(s)	216, 674, 680, 808, 832, 1028, 1351, 1444, 2126
BceAI	ACGGC	2 cut(s)	181, 538
BciT130I	CCWGG	3 cut(s)	1462, 1878, 2009
BciVI	GTATCC	1 cut(s)	2015
BclI	TGATCA	2 cut(s)	862, 967
BcnI	CCSGG	3 cut(s)	612, 707, 825
BcoDI	GTCTC	3 cut(s)	759, 1990, 2039
BfaI	CTAG	3 cut(s)	387, 1203, 1346
BfmI	CTRYAG	1 cut(s)	1287
BfoI	RGCGCY	2 cut(s)	150, 489
BfrI	CTTAAG	1 cut(s)	154
BfuI	GTATCC	1 cut(s)	2015
BglI	GCCNNNNNGGC	3 cut(s)	104, 117, 494
Bme1390I	CCNGG	6 cut(s)	612, 707, 825, 1462, 1878, 2009
Bme18I	GGWCC	6 cut(s)	361, 436, 510, 1417, 1813, 2115
BmeRI	GACNNNNNGTC	1 cut(s)	1273
BmeT110I	CYCGRG	1 cut(s)	2027
BmgT120I	GGNCC	7 cut(s)	361, 436, 510, 1417, 1813, 1874, 2115
BmiI	GGNNCC	9 cut(s)	65, 148, 487, 511, 546, 1466, 1850, 1875, 1923
BmrFI	CCNGG	6 cut(s)	612, 707, 825, 1462, 1878, 2009
BmsI	GCATC	7 cut(s)	38, 366, 810, 897, 1396, 1897, 2086
BoxI	GACNNNNGTC	1 cut(s)	359
BpiI	GAAGAC	1 cut(s)	794
BplI	GAGNNNNNCTC	2 cut(s)	1607, 1639
BpmI	CTGGAG	1 cut(s)	741
Bpu10I	CCTNAGC	3 cut(s)	402, 891, 2052
Bpu14I	TTCGAA	1 cut(s)	785
BpuEI	CTTGAG	2 cut(s)	643, 932
BpuMI	CCSGG	3 cut(s)	612, 707, 825
BsaHI	GRCGYC	3 cut(s)	147, 486, 527
BsaJI	CCNNGG	8 cut(s)	169, 489, 567, 611, 705, 879, 1215, 1278
BsaWI	WCCGGW	3 cut(s)	363, 748, 799
BsaXI	ACNNNNNCTCC	2 cut(s)	1650, 1680
Bsc4I	CCNNNNNNNGG	3 cut(s)	355, 370, 712
Bse118I	RCCGGY	2 cut(s)	107, 143
Bse1I	ACTGG	2 cut(s)	758, 1850
Bse3DI	GCAATG	3 cut(s)	396, 1204, 2073
BseAI	TCCGGA	1 cut(s)	363
BseBI	CCWGG	3 cut(s)	1462, 1878, 2009
BseDI	CCNNGG	8 cut(s)	169, 489, 567, 611, 705, 879, 1215, 1278
BseGI	GGATG	6 cut(s)	381, 691, 819, 1321, 1554, 1727
BseLI	CCNNNNNNNGG	3 cut(s)	355, 370, 712
BseMI	GCAATG	3 cut(s)	396, 1204, 2073
BseMII	CTCAG	3 cut(s)	393, 905, 2043
BseNI	ACTGG	2 cut(s)	758, 1850
BsePI	GCGCGC	2 cut(s)	9, 337
BseRI	GAGGAG	7 cut(s)	194, 246, 249, 252, 419, 734, 755
BseSI	GKGCMC	1 cut(s)	618
BseX3I	CGGCCG	1 cut(s)	105
BseXI	GCAGC	4 cut(s)	1244, 1333, 1617, 1876
BseYI	CCCAGC	1 cut(s)	320
BsgI	GTGCAG	1 cut(s)	960
Bsh1236I	CGCG	6 cut(s)	11, 104, 339, 491, 1355, 2064
Bsh1285I	CGRYCG	1 cut(s)	108
BshFI	GGCC	9 cut(s)	89, 107, 111, 131, 168, 223, 878, 917, 1876
BshNI	GGYRCC	5 cut(s)	146, 485, 544, 1464, 1848
BsiEI	CGRYCG	1 cut(s)	108
BsiHKAI	GWGCWC	2 cut(s)	892, 2058
BsiHKCI	CYCGRG	1 cut(s)	2027
BslFI	GGGAC	2 cut(s)	596, 1632
BslI	CCNNNNNNNGG	3 cut(s)	355, 370, 712
BsmAI	GTCTC	3 cut(s)	759, 1990, 2039
BsmFI	GGGAC	2 cut(s)	596, 1632
BsmI	GAATGC	2 cut(s)	781, 1513
BsnI	GGCC	9 cut(s)	89, 107, 111, 131, 168, 223, 878, 917, 1876
BsoBI	CYCGRG	1 cut(s)	2027
Bsp119I	TTCGAA	1 cut(s)	785
Bsp1286I	GDGCHC	4 cut(s)	618, 699, 892, 2058
Bsp13I	TCCGGA	1 cut(s)	363
Bsp143I	GATC	6 cut(s)	330, 467, 862, 967, 1888, 1921
Bsp19I	CCATGG	1 cut(s)	1278
BspANI	GGCC	9 cut(s)	89, 107, 111, 131, 168, 223, 878, 917, 1876
BspCNI	CTCAG	3 cut(s)	394, 904, 2044
BspEI	TCCGGA	1 cut(s)	363
BspFNI	CGCG	6 cut(s)	11, 104, 339, 491, 1355, 2064
BspHI	TCATGA	1 cut(s)	1390
BspLI	GGNNCC	9 cut(s)	65, 148, 487, 511, 546, 1466, 1850, 1875, 1923
BspPI	GGATC	2 cut(s)	1916, 1929
BspQI	GCTCTTC	2 cut(s)	189, 2063
BspT104I	TTCGAA	1 cut(s)	785
BspT107I	GGYRCC	5 cut(s)	146, 485, 544, 1464, 1848
BspTI	CTTAAG	1 cut(s)	154
BsrBI	CCGCTC	2 cut(s)	58, 271
BsrDI	GCAATG	3 cut(s)	396, 1204, 2073
BsrFI	RCCGGY	2 cut(s)	107, 143
BsrI	ACTGG	2 cut(s)	758, 1850
BssAI	RCCGGY	2 cut(s)	107, 143
BssECI	CCNNGG	8 cut(s)	169, 489, 567, 611, 705, 879, 1215, 1278
BssHII	GCGCGC	2 cut(s)	9, 337
BssMI	GATC	6 cut(s)	330, 467, 862, 967, 1888, 1921
BssNI	GRCGYC	3 cut(s)	147, 486, 527
BssSI	CACGAG	1 cut(s)	978
BssT1I	CCWWGG	3 cut(s)	567, 1215, 1278
Bst2BI	CACGAG	1 cut(s)	978
Bst2UI	CCWGG	3 cut(s)	1462, 1878, 2009
Bst4CI	ACNGT	2 cut(s)	1081, 1291
Bst6I	CTCTTC	7 cut(s)	189, 426, 636, 1630, 1767, 1968, 2063
BstACI	GRCGYC	3 cut(s)	147, 486, 527
BstAFI	CTTAAG	1 cut(s)	154
BstAPI	GCANNNNNTGC	1 cut(s)	1718
BstBI	TTCGAA	1 cut(s)	785
BstC8I	GCNNGC	7 cut(s)	11, 109, 326, 339, 350, 1400, 2048
BstDEI	CTNAG	4 cut(s)	402, 891, 2052, 2111
BstDSI	CCRYGG	3 cut(s)	169, 489, 1278
BstF5I	GGATG	6 cut(s)	381, 691, 819, 1321, 1554, 1727
BstFNI	CGCG	6 cut(s)	11, 104, 339, 491, 1355, 2064
BstH2I	RGCGCY	2 cut(s)	150, 489
BstHHI	GCGC	8 cut(s)	11, 13, 149, 339, 341, 488, 1387, 2066
BstKTI	GATC	6 cut(s)	333, 470, 865, 970, 1891, 1924
BstMAI	GTCTC	3 cut(s)	759, 1990, 2039
BstMBI	GATC	6 cut(s)	330, 467, 862, 967, 1888, 1921
BstMCI	CGRYCG	1 cut(s)	108
BstNI	CCWGG	3 cut(s)	1462, 1878, 2009
BstNSI	RCATGY	4 cut(s)	610, 1273, 1822, 2091
BstPAI	GACNNNNGTC	1 cut(s)	359
BstSCI	CCNGG	6 cut(s)	610, 705, 823, 1460, 1876, 2007
BstSFI	CTRYAG	1 cut(s)	1287
BstSLI	GKGCMC	1 cut(s)	618
BstUI	CGCG	6 cut(s)	11, 104, 339, 491, 1355, 2064
BstV1I	GCAGC	4 cut(s)	1244, 1333, 1617, 1876
BstV2I	GAAGAC	1 cut(s)	794
BstX2I	RGATCY	1 cut(s)	1921
BstXI	CCANNNNNNTGG	1 cut(s)	1084
BstYI	RGATCY	1 cut(s)	1921
BstZI	CGGCCG	1 cut(s)	105
BsuI	GTATCC	1 cut(s)	2015
BsuRI	GGCC	9 cut(s)	89, 107, 111, 131, 168, 223, 878, 917, 1876
BtgI	CCRYGG	3 cut(s)	169, 489, 1278
BtgZI	GCGATG	2 cut(s)	501, 1370
BtsCI	GGATG	6 cut(s)	381, 691, 819, 1321, 1554, 1727
BtsIMutI	CAGTG	3 cut(s)	765, 1077, 1479
Cac8I	GCNNGC	7 cut(s)	11, 109, 326, 339, 350, 1400, 2048
CciI	TCATGA	1 cut(s)	1390
CfoI	GCGC	8 cut(s)	11, 13, 149, 339, 341, 488, 1387, 2066
Cfr10I	RCCGGY	2 cut(s)	107, 143
Cfr13I	GGNCC	7 cut(s)	361, 436, 510, 1417, 1813, 1874, 2115
Cfr42I	CCGCGG	1 cut(s)	492
CseI	GACGC	3 cut(s)	535, 1361, 1634
Csp6I	GTAC	2 cut(s)	1327, 1849
CviQI	GTAC	2 cut(s)	1327, 1849
DdeI	CTNAG	4 cut(s)	402, 891, 2052, 2111
DinI	GGCGCC	2 cut(s)	148, 487
DpnI	GATC	6 cut(s)	332, 469, 864, 969, 1890, 1923
DpnII	GATC	6 cut(s)	330, 467, 862, 967, 1888, 1921
DriI	GACNNNNNGTC	1 cut(s)	1273
EaeI	YGGCCR	5 cut(s)	87, 105, 109, 166, 221
EagI	CGGCCG	1 cut(s)	105
Eam1104I	CTCTTC	7 cut(s)	189, 426, 636, 1630, 1767, 1968, 2063
Eam1105I	GACNNNNNGTC	1 cut(s)	1273
EarI	CTCTTC	7 cut(s)	189, 426, 636, 1630, 1767, 1968, 2063
EciI	GGCGGA	3 cut(s)	255, 276, 731
Ecl136II	GAGCTC	1 cut(s)	2056
EclXI	CGGCCG	1 cut(s)	105
Eco130I	CCWWGG	3 cut(s)	567, 1215, 1278
Eco147I	AGGCCT	1 cut(s)	878
Eco24I	GRGCYC	2 cut(s)	699, 2058
Eco47I	GGWCC	6 cut(s)	361, 436, 510, 1417, 1813, 2115
Eco52I	CGGCCG	1 cut(s)	105
Eco53kI	GAGCTC	1 cut(s)	2056
Eco57I	CTGAAG	3 cut(s)	997, 1424, 2004
Eco88I	CYCGRG	1 cut(s)	2027
EcoICRI	GAGCTC	1 cut(s)	2056
EcoO109I	RGGNCCY	2 cut(s)	510, 1874
EcoRII	CCWGG	3 cut(s)	1460, 1876, 2007
EcoT14I	CCWWGG	3 cut(s)	567, 1215, 1278
EcoT22I	ATGCAT	1 cut(s)	1675
EcoT38I	GRGCYC	2 cut(s)	699, 2058
EgeI	GGCGCC	2 cut(s)	148, 487
EheI	GGCGCC	2 cut(s)	148, 487
ErhI	CCWWGG	3 cut(s)	567, 1215, 1278
FalI	AAGNNNNNCTT	2 cut(s)	1125, 1157
FaqI	GGGAC	2 cut(s)	596, 1632
FauI	CCCGC	1 cut(s)	333
FbaI	TGATCA	2 cut(s)	862, 967
FokI	GGATG	6 cut(s)	388, 698, 826, 1328, 1541, 1714
FriOI	GRGCYC	2 cut(s)	699, 2058
FseI	GGCCGGCC	1 cut(s)	111
FspAI	RTGCGCAY	1 cut(s)	1386
FspBI	CTAG	3 cut(s)	387, 1203, 1346
FspI	TGCGCA	1 cut(s)	1386
GlaI	GCGC	8 cut(s)	10, 12, 148, 338, 340, 487, 1386, 2065
GsaI	CCCAGC	1 cut(s)	324
GsuI	CTGGAG	1 cut(s)	741
HaeII	RGCGCY	2 cut(s)	150, 489
HaeIII	GGCC	9 cut(s)	89, 107, 111, 131, 168, 223, 878, 917, 1876
HgaI	GACGC	3 cut(s)	535, 1361, 1634
HhaI	GCGC	8 cut(s)	11, 13, 149, 339, 341, 488, 1387, 2066
Hin1I	GRCGYC	3 cut(s)	147, 486, 527
Hin6I	GCGC	8 cut(s)	9, 11, 147, 337, 339, 486, 1385, 2064
HinP1I	GCGC	8 cut(s)	9, 11, 147, 337, 339, 486, 1385, 2064
HincII	GTYRAC	1 cut(s)	1267
HindII	GTYRAC	1 cut(s)	1267
HindIII	AAGCTT	3 cut(s)	1529, 1694, 2133
HphI	GGTGA	9 cut(s)	650, 662, 689, 701, 742, 829, 1043, 1892, 1982
Hpy166II	GTNNAC	1 cut(s)	1267
Hpy8I	GTNNAC	1 cut(s)	1267
Hpy99I	CGWCG	2 cut(s)	358, 508
HpyAV	CCTTC	7 cut(s)	109, 532, 629, 706, 1021, 1141, 1804
HpyCH4III	ACNGT	2 cut(s)	1081, 1291
HpyCH4IV	ACGT	2 cut(s)	123, 1497
HpyF3I	CTNAG	4 cut(s)	402, 891, 2052, 2111
HpySE526I	ACGT	2 cut(s)	123, 1497
Hsp92I	GRCGYC	3 cut(s)	147, 486, 527
HspAI	GCGC	8 cut(s)	9, 11, 147, 337, 339, 486, 1385, 2064
KasI	GGCGCC	2 cut(s)	146, 485
Kpn2I	TCCGGA	1 cut(s)	363
KpnI	GGTACC	1 cut(s)	1852
KroI	GCCGGC	1 cut(s)	107
KroNI	GCCGGC	1 cut(s)	109
Ksp22I	TGATCA	2 cut(s)	862, 967
KspI	CCGCGG	1 cut(s)	492
Kzo9I	GATC	6 cut(s)	330, 467, 862, 967, 1888, 1921
LguI	GCTCTTC	2 cut(s)	189, 2063
LmnI	GCTCC	4 cut(s)	55, 1037, 1138, 1789
Lsp1109I	GCAGC	4 cut(s)	1244, 1333, 1617, 1876
LweI	GCATC	7 cut(s)	38, 366, 810, 897, 1396, 1897, 2086
MaeI	CTAG	3 cut(s)	387, 1203, 1346
MaeII	ACGT	2 cut(s)	123, 1497
MaeIII	GTNAC	4 cut(s)	760, 1241, 2022, 2144
MalI	GATC	6 cut(s)	332, 469, 864, 969, 1890, 1923
MbiI	CCGCTC	2 cut(s)	58, 271
MboI	GATC	6 cut(s)	330, 467, 862, 967, 1888, 1921
MfeI	CAATTG	1 cut(s)	774
MflI	RGATCY	1 cut(s)	1921
MhlI	GDGCHC	4 cut(s)	618, 699, 892, 2058
MlsI	TGGCCA	1 cut(s)	89
MluCI	AATT	5 cut(s)	774, 940, 1520, 1653, 1940
MluNI	TGGCCA	1 cut(s)	89
Mly113I	GGCGCC	2 cut(s)	147, 486
MlyI	GAGTC	5 cut(s)	572, 757, 990, 1277, 2041
MmeI	TCCRAC	1 cut(s)	1528
Mox20I	TGGCCA	1 cut(s)	89
Mph1103I	ATGCAT	1 cut(s)	1675
MroI	TCCGGA	1 cut(s)	363
MroNI	GCCGGC	1 cut(s)	107
MroXI	GAANNNNTTC	1 cut(s)	1695
MscI	TGGCCA	1 cut(s)	89
MseI	TTAA	4 cut(s)	155, 872, 1494, 1610
MslI	CAYNNNNRTG	3 cut(s)	1470, 2088, 2123
Msp20I	TGGCCA	1 cut(s)	89
MspA1I	CMGCKG	4 cut(s)	220, 491, 1324, 1402
MspCI	CTTAAG	1 cut(s)	154
MspR9I	CCNGG	6 cut(s)	612, 707, 825, 1462, 1878, 2009
MunI	CAATTG	1 cut(s)	774
Mva1269I	GAATGC	2 cut(s)	781, 1513
MvaI	CCWGG	3 cut(s)	1462, 1878, 2009
MvnI	CGCG	6 cut(s)	11, 104, 339, 491, 1355, 2064
NaeI	GCCGGC	1 cut(s)	109
NarI	GGCGCC	2 cut(s)	147, 486
NciI	CCSGG	3 cut(s)	612, 707, 825
NcoI	CCATGG	1 cut(s)	1278
NdeII	GATC	6 cut(s)	330, 467, 862, 967, 1888, 1921
NgoMIV	GCCGGC	1 cut(s)	107
NlaIV	GGNNCC	9 cut(s)	65, 148, 487, 511, 546, 1466, 1850, 1875, 1923
NmuCI	GTSAC	3 cut(s)	760, 1241, 2022
NsbI	TGCGCA	1 cut(s)	1386
NsiI	ATGCAT	1 cut(s)	1675
NspI	RCATGY	4 cut(s)	610, 1273, 1822, 2091
NspV	TTCGAA	1 cut(s)	785
OliI	CACNNNNGTG	1 cut(s)	2088
PagI	TCATGA	1 cut(s)	1390
PauI	GCGCGC	2 cut(s)	9, 337
PceI	AGGCCT	1 cut(s)	878
PciI	ACATGT	1 cut(s)	2087
PciSI	GCTCTTC	2 cut(s)	189, 2063
PcsI	WCGNNNNNNNCGW	1 cut(s)	985
PctI	GAATGC	2 cut(s)	781, 1513
PdiI	GCCGGC	1 cut(s)	109
PdmI	GAANNNNTTC	1 cut(s)	1695
PflFI	GACNNNGTC	1 cut(s)	508
PleI	GAGTC	5 cut(s)	571, 757, 989, 1277, 2040
PluTI	GGCGCC	2 cut(s)	150, 489
PpsI	GAGTC	5 cut(s)	571, 757, 989, 1277, 2040
PpuMI	RGGWCCY	1 cut(s)	510
PscI	ACATGT	1 cut(s)	2087
PshAI	GACNNNNGTC	1 cut(s)	359
Psp124BI	GAGCTC	1 cut(s)	2058
Psp1406I	AACGTT	1 cut(s)	1497
Psp5II	RGGWCCY	1 cut(s)	510
Psp6I	CCWGG	3 cut(s)	1460, 1876, 2007
PspFI	CCCAGC	1 cut(s)	320
PspGI	CCWGG	3 cut(s)	1460, 1876, 2007
PspN4I	GGNNCC	9 cut(s)	65, 148, 487, 511, 546, 1466, 1850, 1875, 1923
PspPI	GGNCC	7 cut(s)	361, 436, 510, 1417, 1813, 1874, 2115
PspPPI	RGGWCCY	1 cut(s)	510
PsuI	RGATCY	1 cut(s)	1921
PsyI	GACNNNGTC	1 cut(s)	508
PteI	GCGCGC	2 cut(s)	9, 337
PvuII	CAGCTG	2 cut(s)	1324, 1402
RigI	GGCCGGCC	1 cut(s)	111
RsaI	GTAC	2 cut(s)	1328, 1850
RsaNI	GTAC	2 cut(s)	1327, 1849
RseI	CAYNNNNRTG	3 cut(s)	1470, 2088, 2123
SacI	GAGCTC	1 cut(s)	2058
SacII	CCGCGG	1 cut(s)	492
SapI	GCTCTTC	2 cut(s)	189, 2063
SaqAI	TTAA	4 cut(s)	155, 872, 1494, 1610
Sau3AI	GATC	6 cut(s)	330, 467, 862, 967, 1888, 1921
Sau96I	GGNCC	7 cut(s)	361, 436, 510, 1417, 1813, 1874, 2115
SchI	GAGTC	5 cut(s)	572, 757, 990, 1277, 2041
ScrFI	CCNGG	6 cut(s)	612, 707, 825, 1462, 1878, 2009
SduI	GDGCHC	4 cut(s)	618, 699, 892, 2058
SfaNI	GCATC	7 cut(s)	38, 366, 810, 897, 1396, 1897, 2086
SfcI	CTRYAG	1 cut(s)	1287
SfoI	GGCGCC	2 cut(s)	148, 487
Sfr303I	CCGCGG	1 cut(s)	492
SfuI	TTCGAA	1 cut(s)	785
SgrAI	CRCCGGYG	1 cut(s)	143
SgrBI	CCGCGG	1 cut(s)	492
SinI	GGWCC	6 cut(s)	361, 436, 510, 1417, 1813, 2115
SmiMI	CAYNNNNRTG	3 cut(s)	1470, 2088, 2123
SmlI	CTYRAG	3 cut(s)	154, 622, 911
SmoI	CTYRAG	3 cut(s)	154, 622, 911
Sse9I	AATT	5 cut(s)	774, 940, 1520, 1653, 1940
SseBI	AGGCCT	1 cut(s)	878
SspDI	GGCGCC	2 cut(s)	146, 485
SspI	AATATT	1 cut(s)	846
SspMI	CTAG	3 cut(s)	387, 1203, 1346
SstI	GAGCTC	1 cut(s)	2058
StuI	AGGCCT	1 cut(s)	878
StyD4I	CCNGG	6 cut(s)	610, 705, 823, 1460, 1876, 2007
StyI	CCWWGG	3 cut(s)	567, 1215, 1278
TaaI	ACNGT	2 cut(s)	1081, 1291
TaiI	ACGT	2 cut(s)	126, 1500
TasI	AATT	5 cut(s)	774, 940, 1520, 1653, 1940
Tru1I	TTAA	4 cut(s)	155, 872, 1494, 1610
Tru9I	TTAA	4 cut(s)	155, 872, 1494, 1610
TscAI	CASTG	3 cut(s)	765, 1084, 1486
TseFI	GTSAC	3 cut(s)	760, 1241, 2022
TseI	GCWGC	4 cut(s)	1232, 1321, 1605, 1864
Tsp45I	GTSAC	3 cut(s)	760, 1241, 2022
TspGWI	ACGGA	3 cut(s)	186, 371, 2118
TspRI	CASTG	3 cut(s)	765, 1084, 1486
Tth111I	GACNNNGTC	1 cut(s)	508
Vha464I	CTTAAG	1 cut(s)	154
VpaK11BI	GGWCC	6 cut(s)	361, 436, 510, 1417, 1813, 2115
XapI	RAATTY	2 cut(s)	940, 1520
XceI	RCATGY	4 cut(s)	610, 1273, 1822, 2091
XmnI	GAANNNNTTC	1 cut(s)	1695
XspI	CTAG	3 cut(s)	387, 1203, 1346
Zsp2I	ATGCAT	1 cut(s)	1675

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.