MD08G1133200.v1.1

Protein of unknown function (DUF1997)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0009176
Physical Location & Seq
Reverse (-)
12636004 .. 12639084
3081 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1133200.v1.1.491

Sequence Viewer

Length: 765 bp
ATGATGACACAAACAATCAAGTGGCATGAAGGAGTACTGCATGGCTGTGCCTCATTCCCATCCTCTGCTATTCCTATCTTCAACACCAGGAAGAAAATTGAGGTGATAAAGCCAACTTCAAAAGCAACGAAGTTTATAAGTTCAGAGTCAAATGGAAATAAAGTAGCAAATTTGTGTGCTACAAAGAAGGAAAGAATTAAGCTGCCAAGTTACGATGTCGGAAGCAAGAGTTTTCACATCAGTGAGTTTTTGAGCCACCAATCAGGAATTGAAGCCATGCTCAACACCAGAGCCTTGGAAAGTTTTCAATCCCTTGATGCTGATACTTATAGGTGCACTCTGCCAAAACTTAAGCTTTTGAATTATGAAGCTGCTCCAGTTCTGGACTTGAGAGTAACCCCAACAAATGAAGATTGTATAGTTGAGATGCTTTCTTGCAGGTTTGAGGGTTCGGAAGCCTTGGAACGCCAAAACAGCCATTTTTCAGCATTCATGACTAATCACATGTCGTGGGACACGAACAACTCTGAATCGTTTTTGGAGGTTGATGTGAAGTTGCAACTCACACTTGAGATATATACACGCCCCTTTAATATGATGCCGGTATCAGCTGTTGAGCGTCCTGGAAATCTAATGATGCAAGCGTTGGTAGATCGGCTGGTGCCATTGCTTCTGCAGCAACTACTACAAGATTATGGAAAATGGATTGATGAACAGTTACAGGATGTCTCAAAAAGTGGTCCATTCATGCATAGAGAGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1997

Protein Analysis

255

Amino Acids

28.9

Weight (kDa)

6.39

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1997 PF09366 80 - 235 2.1e-38 Protein of unknown function (DUF1997)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 137
Acc36I ACCTGC 1 cut(s) 429
AccB1I GGYRCC 1 cut(s) 661
AcsI RAATTY 1 cut(s) 169
AfaI GTAC 1 cut(s) 36
AflII CTTAAG 1 cut(s) 350
AflIII ACRYGT 1 cut(s) 504
AgsI TTSAA 5 cut(s) 82, 120, 272, 308, 361
AjnI CCWGG 2 cut(s) 86, 622
AleI CACNNNNGTG 1 cut(s) 240
AluBI AGCT 4 cut(s) 202, 355, 371, 611
AluI AGCT 4 cut(s) 202, 355, 371, 611
Alw21I GWGCWC 1 cut(s) 338
Alw26I GTCTC 1 cut(s) 733
Alw44I GTGCAC 1 cut(s) 334
ApaLI GTGCAC 1 cut(s) 334
ApeKI GCWGC 3 cut(s) 202, 371, 676
ApoI RAATTY 1 cut(s) 169
Asp700I GAANNNNTTC 1 cut(s) 303
AspS9I GGNCC 1 cut(s) 740
AsuHPI GGTGA 1 cut(s) 115
AvaII GGWCC 1 cut(s) 740
BaeGI GKGCMC 1 cut(s) 338
BanI GGYRCC 1 cut(s) 661
Bbv12I GWGCWC 1 cut(s) 338
BbvI GCAGC 3 cut(s) 189, 358, 688
BccI CCATC 1 cut(s) 67
BciT130I CCWGG 2 cut(s) 88, 624
BcoDI GTCTC 1 cut(s) 733
BfmI CTRYAG 1 cut(s) 674
BfrI CTTAAG 1 cut(s) 350
BfuAI ACCTGC 1 cut(s) 429
BisI GCNGC 3 cut(s) 203, 372, 677
BlsI GCNGC 3 cut(s) 204, 373, 678
BmcAI AGTACT 1 cut(s) 36
Bme1390I CCNGG 2 cut(s) 88, 624
Bme18I GGWCC 1 cut(s) 740
BmgT120I GGNCC 1 cut(s) 740
BmiI GGNNCC 1 cut(s) 663
BmrFI CCNGG 2 cut(s) 88, 624
BmsI GCATC 4 cut(s) 307, 417, 588, 627
BpmI CTGGAG 1 cut(s) 360
BpuEI CTTGAG 2 cut(s) 409, 590
BsaJI CCNNGG 2 cut(s) 294, 459
Bse118I RCCGGY 1 cut(s) 601
Bse1I ACTGG 1 cut(s) 377
Bse3DI GCAATG 1 cut(s) 665
BseBI CCWGG 2 cut(s) 88, 624
BseDI CCNNGG 2 cut(s) 294, 459
BseGI GGATG 2 cut(s) 59, 730
BseMI GCAATG 1 cut(s) 665
BseNI ACTGG 1 cut(s) 377
BseSI GKGCMC 1 cut(s) 338
BseXI GCAGC 3 cut(s) 189, 358, 688
BshNI GGYRCC 1 cut(s) 661
BsiHKAI GWGCWC 1 cut(s) 338
BsiSI CCGG 1 cut(s) 602
BslFI GGGAC 1 cut(s) 527
BsmAI GTCTC 1 cut(s) 733
BsmFI GGGAC 1 cut(s) 527
BsmI GAATGC 1 cut(s) 488
Bsp1286I GDGCHC 1 cut(s) 338
Bsp143I GATC 1 cut(s) 652
BspHI TCATGA 1 cut(s) 492
BspLI GGNNCC 1 cut(s) 663
BspMAI CTGCAG 1 cut(s) 678
BspMI ACCTGC 1 cut(s) 429
BspT107I GGYRCC 1 cut(s) 661
BspTI CTTAAG 1 cut(s) 350
BsrDI GCAATG 1 cut(s) 665
BsrFI RCCGGY 1 cut(s) 601
BsrI ACTGG 1 cut(s) 377
BssAI RCCGGY 1 cut(s) 601
BssECI CCNNGG 2 cut(s) 294, 459
BssMI GATC 1 cut(s) 652
BssT1I CCWWGG 2 cut(s) 294, 459
Bst2UI CCWGG 2 cut(s) 88, 624
Bst4CI ACNGT 1 cut(s) 717
BstAFI CTTAAG 1 cut(s) 350
BstC8I GCNNGC 1 cut(s) 642
BstF5I GGATG 2 cut(s) 59, 730
BstKTI GATC 1 cut(s) 655
BstMAI GTCTC 1 cut(s) 733
BstMBI GATC 1 cut(s) 652
BstMWI GCNNNNNNNGC 2 cut(s) 474, 676
BstNI CCWGG 2 cut(s) 88, 624
BstNSI RCATGY 1 cut(s) 508
BstSCI CCNGG 2 cut(s) 86, 622
BstSFI CTRYAG 1 cut(s) 674
BstSLI GKGCMC 1 cut(s) 338
BstV1I GCAGC 3 cut(s) 189, 358, 688
BstXI CCANNNNNNTGG 1 cut(s) 295
BtsCI GGATG 2 cut(s) 59, 730
BtsIMutI CAGTG 1 cut(s) 247
BveI ACCTGC 1 cut(s) 429
Cac8I GCNNGC 1 cut(s) 642
CciI TCATGA 1 cut(s) 492
Cfr10I RCCGGY 1 cut(s) 601
Cfr13I GGNCC 1 cut(s) 740
CseI GACGC 1 cut(s) 608
Csp6I GTAC 1 cut(s) 35
CviAII CATG 6 cut(s) 26, 41, 277, 493, 505, 748
CviQI GTAC 1 cut(s) 35
DpnI GATC 1 cut(s) 654
DpnII GATC 1 cut(s) 652
Eco130I CCWWGG 2 cut(s) 294, 459
Eco47I GGWCC 1 cut(s) 740
EcoRII CCWGG 2 cut(s) 86, 622
EcoT14I CCWWGG 2 cut(s) 294, 459
EcoT22I ATGCAT 1 cut(s) 753
ErhI CCWWGG 2 cut(s) 294, 459
FaeI CATG 6 cut(s) 29, 44, 280, 496, 508, 751
FaqI GGGAC 1 cut(s) 527
FatI CATG 6 cut(s) 25, 40, 276, 492, 504, 747
Fnu4HI GCNGC 3 cut(s) 203, 372, 677
FokI GGATG 2 cut(s) 46, 737
Fsp4HI GCNGC 3 cut(s) 203, 372, 677
GluI GCNGC 3 cut(s) 203, 372, 677
GsuI CTGGAG 1 cut(s) 360
HapII CCGG 1 cut(s) 602
HgaI GACGC 1 cut(s) 608
Hin1II CATG 6 cut(s) 29, 44, 280, 496, 508, 751
HindIII AAGCTT 1 cut(s) 353
HinfI GANTC 2 cut(s) 146, 530
HpaII CCGG 1 cut(s) 602
HphI GGTGA 1 cut(s) 115
Hpy166II GTNNAC 1 cut(s) 336
Hpy188I TCNGA 4 cut(s) 145, 221, 454, 529
Hpy188III TCNNGA 3 cut(s) 264, 383, 493
Hpy8I GTNNAC 1 cut(s) 336
HpyAV CCTTC 2 cut(s) 23, 181
HpyCH4III ACNGT 1 cut(s) 717
HpyCH4V TGCA 7 cut(s) 40, 336, 438, 559, 640, 676, 751
HpyF10VI GCNNNNNNNGC 2 cut(s) 474, 676
Hsp92II CATG 6 cut(s) 29, 44, 280, 496, 508, 751
Kzo9I GATC 1 cut(s) 652
LmnI GCTCC 1 cut(s) 379
Lsp1109I GCAGC 3 cut(s) 189, 358, 688
LweI GCATC 4 cut(s) 307, 417, 588, 627
MaeIII GTNAC 3 cut(s) 209, 394, 717
MalI GATC 1 cut(s) 654
MboI GATC 1 cut(s) 652
MboII GAAGA 3 cut(s) 70, 103, 422
MhlI GDGCHC 1 cut(s) 338
MluCI AATT 5 cut(s) 96, 169, 195, 267, 361
MlyI GAGTC 1 cut(s) 155
MmeI TCCRAC 1 cut(s) 199
MnlI CCTC 6 cut(s) 61, 73, 94, 439, 535, 753
Mph1103I ATGCAT 1 cut(s) 753
MroXI GAANNNNTTC 1 cut(s) 303
MseI TTAA 3 cut(s) 198, 351, 591
MslI CAYNNNNRTG 2 cut(s) 45, 240
MspA1I CMGCKG 1 cut(s) 611
MspCI CTTAAG 1 cut(s) 350
MspI CCGG 1 cut(s) 602
MspR9I CCNGG 2 cut(s) 88, 624
Mva1269I GAATGC 1 cut(s) 488
MvaI CCWGG 2 cut(s) 88, 624
MwoI GCNNNNNNNGC 2 cut(s) 474, 676
NdeII GATC 1 cut(s) 652
NlaIII CATG 6 cut(s) 29, 44, 280, 496, 508, 751
NlaIV GGNNCC 1 cut(s) 663
NsiI ATGCAT 1 cut(s) 753
NspI RCATGY 1 cut(s) 508
OliI CACNNNNGTG 1 cut(s) 240
PagI TCATGA 1 cut(s) 492
PciI ACATGT 1 cut(s) 504
PcsI WCGNNNNNNNCGW 1 cut(s) 515
PctI GAATGC 1 cut(s) 488
PdmI GAANNNNTTC 1 cut(s) 303
PfeI GAWTC 1 cut(s) 530
PfoI TCCNGGA 1 cut(s) 622
PkrI GCNGC 3 cut(s) 204, 373, 678
PleI GAGTC 1 cut(s) 154
PpsI GAGTC 1 cut(s) 154
PscI ACATGT 1 cut(s) 504
PsiI TTATAA 1 cut(s) 137
Psp6I CCWGG 2 cut(s) 86, 622
PspGI CCWGG 2 cut(s) 86, 622
PspN4I GGNNCC 1 cut(s) 663
PspPI GGNCC 1 cut(s) 740
PstI CTGCAG 1 cut(s) 678
PvuII CAGCTG 1 cut(s) 611
RsaI GTAC 1 cut(s) 36
RsaNI GTAC 1 cut(s) 35
RseI CAYNNNNRTG 2 cut(s) 45, 240
SaqAI TTAA 3 cut(s) 198, 351, 591
SatI GCNGC 3 cut(s) 203, 372, 677
Sau3AI GATC 1 cut(s) 652
Sau96I GGNCC 1 cut(s) 740
ScaI AGTACT 1 cut(s) 36
SchI GAGTC 1 cut(s) 155
ScrFI CCNGG 2 cut(s) 88, 624
SduI GDGCHC 1 cut(s) 338
SetI ASST 9 cut(s) 105, 204, 335, 357, 373, 443, 546, 613, 764
SfaNI GCATC 4 cut(s) 307, 417, 588, 627
SfcI CTRYAG 1 cut(s) 674
SinI GGWCC 1 cut(s) 740
SmiMI CAYNNNNRTG 2 cut(s) 45, 240
SmlI CTYRAG 3 cut(s) 350, 388, 569
SmoI CTYRAG 3 cut(s) 350, 388, 569
Sse9I AATT 5 cut(s) 96, 169, 195, 267, 361
StyD4I CCNGG 2 cut(s) 86, 622
StyI CCWWGG 2 cut(s) 294, 459
TaaI ACNGT 1 cut(s) 717
TasI AATT 5 cut(s) 96, 169, 195, 267, 361
TatI WGTACW 1 cut(s) 34
TfiI GAWTC 1 cut(s) 530
Tru1I TTAA 3 cut(s) 198, 351, 591
Tru9I TTAA 3 cut(s) 198, 351, 591
TscAI CASTG 1 cut(s) 247
TseI GCWGC 3 cut(s) 202, 371, 676
TspDTI ATGAA 6 cut(s) 42, 381, 423, 481, 726, 736
TspRI CASTG 1 cut(s) 247
Vha464I CTTAAG 1 cut(s) 350
VneI GTGCAC 1 cut(s) 334
VpaK11BI GGWCC 1 cut(s) 740
XapI RAATTY 1 cut(s) 169
XceI RCATGY 1 cut(s) 508
XmnI GAANNNNTTC 1 cut(s) 303
ZrmI AGTACT 1 cut(s) 36
Zsp2I ATGCAT 1 cut(s) 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.