RLG00000008431

Protein of unknown function (DUF1997)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0009176
Physical Location & Seq
Forward (+)
30462974 .. 30464834
1861 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008431

Sequence Viewer

Length: 759 bp
ATGTCACTAAGTATCAAGTGGCATGAACAACTAAATGGCTTTACTTCATTCCCATCCCCTGCTACTCCAATCTTCAATACCAGGAAGAAAGTTGAAGTGATAAGGGTATTAGCTAAAGCAGCCAACTTGGGTTCTGAATCAAATGCAAAGAAAGCAAATTTGTGTGCTGCAAAGAAGGAAAGAATTAAGCTGCCAAGTTTTGATGGTAACTTAGGGCGCAAGAATTTTCACATTAGAGAGTTTCTAAGCCACCCATCAGGAACTGAAGCCATGCTCAATACTAGAGCACTGGAAAATTTTGAACCCCTTTGCACTGATACTTACAGGTGCACTTTGCCAAAGCTTAAACTTTTGAATTTTGAAGCAGCCCCTGTGGTGGATTTAAGGGTGAACCCAACCAATGAGGATTGTATGGTTGAGATGCTCTCTTGCAAGTTTGAGGGTTCAGAATCTGTGGAACGCCAAAACAGCCGTTTTTCAGCACTGATGACAAATCACATGTCGTGGGGGACAGACGACTCTGAATCATTTTTGGAAGTTGATGTGAAGTTAAATCTCACCCTCGAGATCTATACGCGCCCTTTCACCATGATGCCGGTATCAGCTGTTGAGCGGCCGGGAAACTTCAACTCCTACAAGATTATGACAAATGGGTTCATAAGCAATCAGAAGATGATCTTCCATGAAATGGTCCATTTCTGCATTGTCAAGATGGCCATTGGTAGAACCAAAAAAGACACAGCGGTGGCATTACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1997

Protein Analysis

253

Amino Acids

28.49

Weight (kDa)

8.86

Isoelectric Point (pI)

33.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1997 PF09366 78 - 208 3.1e-27 Protein of unknown function (DUF1997)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 688
AccBSI CCGCTC 1 cut(s) 613
AccII CGCG 1 cut(s) 577
AciI CCGC 2 cut(s) 613, 743
AcoI YGGCCR 2 cut(s) 614, 714
AcsI RAATTY 4 cut(s) 157, 223, 295, 355
AcuI CTGAAG 1 cut(s) 285
AfiI CCNNNNNNNGG 2 cut(s) 376, 688
AflIII ACRYGT 1 cut(s) 498
AgsI TTSAA 6 cut(s) 76, 95, 302, 355, 362, 628
AjnI CCWGG 1 cut(s) 80
AleI CACNNNNGTG 1 cut(s) 743
AluBI AGCT 4 cut(s) 113, 190, 343, 605
AluI AGCT 4 cut(s) 113, 190, 343, 605
Alw21I GWGCWC 2 cut(s) 289, 332
Alw44I GTGCAC 1 cut(s) 328
AlwNI CAGNNNCTG 3 cut(s) 263, 371, 452
Ama87I CYCGRG 1 cut(s) 563
AoxI GGCC 2 cut(s) 614, 714
ApaLI GTGCAC 1 cut(s) 328
ApeKI GCWGC 4 cut(s) 119, 167, 190, 365
ApoI RAATTY 4 cut(s) 157, 223, 295, 355
ArsI GACNNNNNNTTYG 2 cut(s) 485, 517
AspLEI GCGC 2 cut(s) 219, 579
AspS9I GGNCC 1 cut(s) 691
AsuC2I CCSGG 1 cut(s) 618
AsuHPI GGTGA 3 cut(s) 400, 550, 577
AvaI CYCGRG 1 cut(s) 563
AvaII GGWCC 1 cut(s) 691
BaeGI GKGCMC 1 cut(s) 332
BalI TGGCCA 1 cut(s) 716
Bbv12I GWGCWC 2 cut(s) 289, 332
BbvI GCAGC 4 cut(s) 131, 154, 177, 377
BccI CCATC 4 cut(s) 61, 197, 262, 706
BceAI ACGGC 1 cut(s) 456
BciT130I CCWGG 1 cut(s) 82
BcnI CCSGG 1 cut(s) 618
BfaI CTAG 2 cut(s) 282, 757
BglII AGATCT 1 cut(s) 567
BisI GCNGC 5 cut(s) 120, 168, 191, 366, 614
BlsI GCNGC 5 cut(s) 121, 169, 192, 367, 615
Bme1390I CCNGG 2 cut(s) 82, 618
Bme18I GGWCC 1 cut(s) 691
BmeT110I CYCGRG 1 cut(s) 563
BmgT120I GGNCC 1 cut(s) 691
BmrFI CCNGG 2 cut(s) 82, 618
BmsI GCATC 2 cut(s) 411, 582
BplI GAGNNNNNCTC 2 cut(s) 410, 442
BpuMI CCSGG 1 cut(s) 618
BsaXI ACNNNNNCTCC 2 cut(s) 614, 644
Bsc4I CCNNNNNNNGG 2 cut(s) 376, 688
Bse118I RCCGGY 1 cut(s) 595
Bse1I ACTGG 1 cut(s) 294
BseBI CCWGG 1 cut(s) 82
BseGI GGATG 1 cut(s) 53
BseLI CCNNNNNNNGG 2 cut(s) 376, 688
BseNI ACTGG 1 cut(s) 294
BseSI GKGCMC 1 cut(s) 332
BseX3I CGGCCG 1 cut(s) 614
BseXI GCAGC 4 cut(s) 131, 154, 177, 377
Bsh1236I CGCG 1 cut(s) 577
Bsh1285I CGRYCG 1 cut(s) 617
BshFI GGCC 2 cut(s) 616, 716
BsiEI CGRYCG 1 cut(s) 617
BsiHKAI GWGCWC 2 cut(s) 289, 332
BsiHKCI CYCGRG 1 cut(s) 563
BsiSI CCGG 2 cut(s) 596, 617
BslFI GGGAC 1 cut(s) 523
BslI CCNNNNNNNGG 2 cut(s) 376, 688
BsmFI GGGAC 1 cut(s) 523
BsnI GGCC 2 cut(s) 616, 716
BsoBI CYCGRG 1 cut(s) 563
Bsp1286I GDGCHC 2 cut(s) 289, 332
Bsp143I GATC 2 cut(s) 567, 675
BspACI CCGC 2 cut(s) 613, 743
BspANI GGCC 2 cut(s) 616, 716
BspFNI CGCG 1 cut(s) 577
BsrBI CCGCTC 1 cut(s) 613
BsrFI RCCGGY 1 cut(s) 595
BsrI ACTGG 1 cut(s) 294
BssAI RCCGGY 1 cut(s) 595
BssMI GATC 2 cut(s) 567, 675
Bst2UI CCWGG 1 cut(s) 82
BstDEI CTNAG 3 cut(s) 8, 211, 245
BstF5I GGATG 1 cut(s) 53
BstFNI CGCG 1 cut(s) 577
BstHHI GCGC 2 cut(s) 219, 579
BstKTI GATC 2 cut(s) 570, 678
BstMBI GATC 2 cut(s) 567, 675
BstMCI CGRYCG 1 cut(s) 617
BstMWI GCNNNNNNNGC 3 cut(s) 119, 152, 468
BstNI CCWGG 1 cut(s) 82
BstNSI RCATGY 1 cut(s) 502
BstSCI CCNGG 2 cut(s) 80, 616
BstSLI GKGCMC 1 cut(s) 332
BstUI CGCG 1 cut(s) 577
BstV1I GCAGC 4 cut(s) 131, 154, 177, 377
BstX2I RGATCY 1 cut(s) 567
BstYI RGATCY 1 cut(s) 567
BstZI CGGCCG 1 cut(s) 614
BsuRI GGCC 2 cut(s) 616, 716
BtsCI GGATG 1 cut(s) 53
BtsIMutI CAGTG 3 cut(s) 287, 312, 482
CaiI CAGNNNCTG 3 cut(s) 263, 371, 452
CfoI GCGC 2 cut(s) 219, 579
Cfr10I RCCGGY 1 cut(s) 595
Cfr13I GGNCC 1 cut(s) 691
CviAII CATG 5 cut(s) 23, 271, 499, 589, 683
DdeI CTNAG 3 cut(s) 8, 211, 245
DpnI GATC 2 cut(s) 569, 677
DpnII GATC 2 cut(s) 567, 675
EaeI YGGCCR 2 cut(s) 614, 714
EagI CGGCCG 1 cut(s) 614
EclXI CGGCCG 1 cut(s) 614
Eco47I GGWCC 1 cut(s) 691
Eco52I CGGCCG 1 cut(s) 614
Eco57I CTGAAG 1 cut(s) 285
Eco88I CYCGRG 1 cut(s) 563
EcoRII CCWGG 1 cut(s) 80
FaeI CATG 5 cut(s) 26, 274, 502, 592, 686
FaiI YATR 9 cut(s) 24, 272, 413, 500, 573, 590, 644, 659, 684
FalI AAGNNNNNCTT 2 cut(s) 662, 694
FaqI GGGAC 1 cut(s) 523
FatI CATG 5 cut(s) 22, 270, 498, 588, 682
Fnu4HI GCNGC 5 cut(s) 120, 168, 191, 366, 614
FokI GGATG 1 cut(s) 40
Fsp4HI GCNGC 5 cut(s) 120, 168, 191, 366, 614
FspBI CTAG 2 cut(s) 282, 757
GlaI GCGC 2 cut(s) 218, 578
GluI GCNGC 5 cut(s) 120, 168, 191, 366, 614
HaeIII GGCC 2 cut(s) 616, 716
HapII CCGG 2 cut(s) 596, 617
HhaI GCGC 2 cut(s) 219, 579
Hin1II CATG 5 cut(s) 26, 274, 502, 592, 686
Hin6I GCGC 2 cut(s) 217, 577
HinP1I GCGC 2 cut(s) 217, 577
HindIII AAGCTT 1 cut(s) 341
HinfI GANTC 4 cut(s) 137, 449, 518, 524
HpaII CCGG 2 cut(s) 596, 617
HphI GGTGA 3 cut(s) 400, 550, 577
Hpy166II GTNNAC 2 cut(s) 330, 391
Hpy188I TCNGA 4 cut(s) 136, 448, 523, 669
Hpy188III TCNNGA 3 cut(s) 258, 565, 709
Hpy8I GTNNAC 2 cut(s) 330, 391
HpyAV CCTTC 1 cut(s) 169
HpyCH4V TGCA 6 cut(s) 146, 170, 312, 330, 432, 702
HpyF10VI GCNNNNNNNGC 3 cut(s) 119, 152, 468
HpyF3I CTNAG 3 cut(s) 8, 211, 245
Hsp92II CATG 5 cut(s) 26, 274, 502, 592, 686
HspAI GCGC 2 cut(s) 217, 577
Kzo9I GATC 2 cut(s) 567, 675
LpnPI CCDG 9 cut(s) 67, 72, 94, 243, 275, 310, 384, 609, 630
Lsp1109I GCAGC 4 cut(s) 131, 154, 177, 377
LweI GCATC 2 cut(s) 411, 582
MaeI CTAG 2 cut(s) 282, 757
MaeIII GTNAC 2 cut(s) 3, 206
MalI GATC 2 cut(s) 569, 677
MbiI CCGCTC 1 cut(s) 613
MboI GATC 2 cut(s) 567, 675
MboII GAAGA 4 cut(s) 64, 97, 670, 682
MflI RGATCY 1 cut(s) 567
MhlI GDGCHC 2 cut(s) 289, 332
MlsI TGGCCA 1 cut(s) 716
MluCI AATT 5 cut(s) 157, 183, 223, 295, 355
MluNI TGGCCA 1 cut(s) 716
MlyI GAGTC 1 cut(s) 512
MnlI CCTC 3 cut(s) 397, 433, 572
Mox20I TGGCCA 1 cut(s) 716
MscI TGGCCA 1 cut(s) 716
MseI TTAA 4 cut(s) 186, 345, 383, 551
MslI CAYNNNNRTG 2 cut(s) 590, 743
Msp20I TGGCCA 1 cut(s) 716
MspA1I CMGCKG 2 cut(s) 605, 743
MspI CCGG 2 cut(s) 596, 617
MspR9I CCNGG 2 cut(s) 82, 618
MvaI CCWGG 1 cut(s) 82
MvnI CGCG 1 cut(s) 577
MwoI GCNNNNNNNGC 3 cut(s) 119, 152, 468
NciI CCSGG 1 cut(s) 618
NdeII GATC 2 cut(s) 567, 675
NlaIII CATG 5 cut(s) 26, 274, 502, 592, 686
NmuCI GTSAC 1 cut(s) 3
NspI RCATGY 1 cut(s) 502
OliI CACNNNNGTG 1 cut(s) 743
PaeR7I CTCGAG 1 cut(s) 563
PciI ACATGT 1 cut(s) 498
PfeI GAWTC 3 cut(s) 137, 449, 524
PflMI CCANNNNNTGG 1 cut(s) 688
PkrI GCNGC 5 cut(s) 121, 169, 192, 367, 615
PleI GAGTC 1 cut(s) 512
PpsI GAGTC 1 cut(s) 512
PscI ACATGT 1 cut(s) 498
Psp6I CCWGG 1 cut(s) 80
PspGI CCWGG 1 cut(s) 80
PspPI GGNCC 1 cut(s) 691
PstNI CAGNNNCTG 3 cut(s) 263, 371, 452
PsuI RGATCY 1 cut(s) 567
PvuII CAGCTG 1 cut(s) 605
RseI CAYNNNNRTG 2 cut(s) 590, 743
SaqAI TTAA 4 cut(s) 186, 345, 383, 551
SatI GCNGC 5 cut(s) 120, 168, 191, 366, 614
Sau3AI GATC 2 cut(s) 567, 675
Sau96I GGNCC 1 cut(s) 691
SchI GAGTC 1 cut(s) 512
ScrFI CCNGG 2 cut(s) 82, 618
SduI GDGCHC 2 cut(s) 289, 332
SetI ASST 5 cut(s) 115, 192, 329, 345, 607
SfaNI GCATC 2 cut(s) 411, 582
Sfr274I CTCGAG 1 cut(s) 563
SinI GGWCC 1 cut(s) 691
SlaI CTCGAG 1 cut(s) 563
SmiMI CAYNNNNRTG 2 cut(s) 590, 743
SmlI CTYRAG 1 cut(s) 563
SmoI CTYRAG 1 cut(s) 563
Sse9I AATT 5 cut(s) 157, 183, 223, 295, 355
SsiI CCGC 2 cut(s) 613, 743
SspMI CTAG 2 cut(s) 282, 757
StyD4I CCNGG 2 cut(s) 80, 616
TaqI TCGA 1 cut(s) 564
TasI AATT 5 cut(s) 157, 183, 223, 295, 355
TauI GCSGC 1 cut(s) 616
TfiI GAWTC 3 cut(s) 137, 449, 524
Tru1I TTAA 4 cut(s) 186, 345, 383, 551
Tru9I TTAA 4 cut(s) 186, 345, 383, 551
TscAI CASTG 3 cut(s) 294, 319, 489
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 4 cut(s) 119, 167, 190, 365
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 4 cut(s) 36, 39, 646, 699
TspRI CASTG 3 cut(s) 294, 319, 489
Van91I CCANNNNNTGG 1 cut(s) 688
VneI GTGCAC 1 cut(s) 328
VpaK11BI GGWCC 1 cut(s) 691
XapI RAATTY 4 cut(s) 157, 223, 295, 355
XceI RCATGY 1 cut(s) 502
XhoI CTCGAG 1 cut(s) 563
XspI CTAG 2 cut(s) 282, 757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.