Rroxscaffold_5G00355220

Protein of unknown function (DUF1997)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0009176
Physical Location & Seq
Reverse (-)
33848391 .. 33850728
2338 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00355220.1

Sequence Viewer

Length: 738 bp
ATGTCACTAAGTATCAAGTGGCATGAACAACTACATGGCTTTACTTCATTCCCATCCCCTGCTACTCCAATCTTCAATACCAGGAAGAAGGTTGAAGTGATAAGGGTATTAGCTAAAGCAGCCAACTTCAACTTGGGTTCTGAATCAAATGCAAAGAAAGCAAATTTGTGTGCTGCAAAGAAGGAAAGAATTAAGCTGCCAAGTTTTGATGGTAACTTAGGGCGCAAGAATTTTCACATTAGAGAGTTTCTAAGCCACCCATCAGGAACTGAAGCCATGCTCAATACTAGAGCACTGGAAAATTTTGAACCCCTTAGCGCTGATACTTACAGGTGCATTTTGCCAAAGCTTAAACTTTTGAATTTTGAAGCAGCCCCTGTGGTGGATTTAAGAGTGAACCCAACCAATGAGGATTGTATGGTTGAGATGCTCTCTTGCAAGTTTGAGGGTTCAGAATCTGTGGAACGCCAAAACAGCCATTTTTCAGCATTGATGACAAATCACATGTCGTGGGGGACAGACGACTCTGAATCATTTTTGGAAGTTGATGTGAAGTTAAATCTCACCCTCGAGATCTATACGCCCCCTTTTACCATGATGCCGGTATCAGCTGTTGAGCGTCCGGGAAACTTAATGATGCAAGCATTACTGGATAGGCTGGTCCCACTGCTTCTGCAGCAACTCCTACAAGATTATGACAAATGGGTTCATAAGCATTCAGAAGATGATCTTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1997

Protein Analysis

245

Amino Acids

27.8

Weight (kDa)

6.08

Isoelectric Point (pI)

39.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1997 PF09366 80 - 236 5e-38 Protein of unknown function (DUF1997)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 4 cut(s) 163, 229, 301, 361
AcuI CTGAAG 1 cut(s) 291
AfeI AGCGCT 1 cut(s) 319
AfiI CCNNNNNNNGG 1 cut(s) 382
AflIII ACRYGT 1 cut(s) 504
AgsI TTSAA 6 cut(s) 76, 95, 130, 308, 361, 368
AjnI CCWGG 1 cut(s) 80
AluBI AGCT 4 cut(s) 113, 196, 349, 611
AluI AGCT 4 cut(s) 113, 196, 349, 611
Alw21I GWGCWC 1 cut(s) 295
AlwNI CAGNNNCTG 3 cut(s) 269, 377, 458
Ama87I CYCGRG 1 cut(s) 569
Aor51HI AGCGCT 1 cut(s) 319
ApeKI GCWGC 5 cut(s) 119, 173, 196, 371, 676
ApoI RAATTY 4 cut(s) 163, 229, 301, 361
ArsI GACNNNNNNTTYG 2 cut(s) 491, 523
AspLEI GCGC 2 cut(s) 225, 320
AspS9I GGNCC 1 cut(s) 661
AsuC2I CCSGG 1 cut(s) 624
AsuHPI GGTGA 1 cut(s) 556
AvaI CYCGRG 1 cut(s) 569
AvaII GGWCC 1 cut(s) 661
Bbv12I GWGCWC 1 cut(s) 295
BbvI GCAGC 5 cut(s) 131, 160, 183, 383, 688
BccI CCATC 3 cut(s) 61, 203, 268
BciT130I CCWGG 1 cut(s) 82
BcnI CCSGG 1 cut(s) 624
BfaI CTAG 1 cut(s) 288
BfmI CTRYAG 1 cut(s) 674
BfoI RGCGCY 1 cut(s) 321
BglII AGATCT 1 cut(s) 573
BisI GCNGC 5 cut(s) 120, 174, 197, 372, 677
BlsI GCNGC 5 cut(s) 121, 175, 198, 373, 678
Bme1390I CCNGG 2 cut(s) 82, 624
Bme18I GGWCC 1 cut(s) 661
BmeT110I CYCGRG 1 cut(s) 569
BmgT120I GGNCC 1 cut(s) 661
BmiI GGNNCC 1 cut(s) 663
BmrFI CCNGG 2 cut(s) 82, 624
BmsI GCATC 3 cut(s) 417, 588, 627
BplI GAGNNNNNCTC 2 cut(s) 416, 448
Bpu10I CCTNAGC 1 cut(s) 314
BpuMI CCSGG 1 cut(s) 624
Bsc4I CCNNNNNNNGG 1 cut(s) 382
Bse118I RCCGGY 1 cut(s) 601
Bse1I ACTGG 2 cut(s) 300, 654
BseBI CCWGG 1 cut(s) 82
BseGI GGATG 1 cut(s) 53
BseLI CCNNNNNNNGG 1 cut(s) 382
BseNI ACTGG 2 cut(s) 300, 654
BseXI GCAGC 5 cut(s) 131, 160, 183, 383, 688
BsiHKAI GWGCWC 1 cut(s) 295
BsiHKCI CYCGRG 1 cut(s) 569
BsiSI CCGG 2 cut(s) 602, 623
BslFI GGGAC 2 cut(s) 529, 647
BslI CCNNNNNNNGG 1 cut(s) 382
BsmFI GGGAC 2 cut(s) 529, 647
BsmI GAATGC 1 cut(s) 715
BsoBI CYCGRG 1 cut(s) 569
Bsp1286I GDGCHC 1 cut(s) 295
Bsp143I GATC 2 cut(s) 573, 727
BspLI GGNNCC 1 cut(s) 663
BspMAI CTGCAG 1 cut(s) 678
BsrFI RCCGGY 1 cut(s) 601
BsrI ACTGG 2 cut(s) 300, 654
BssAI RCCGGY 1 cut(s) 601
BssMI GATC 2 cut(s) 573, 727
Bst2UI CCWGG 1 cut(s) 82
BstC8I GCNNGC 1 cut(s) 642
BstDEI CTNAG 4 cut(s) 8, 217, 251, 314
BstF5I GGATG 1 cut(s) 53
BstH2I RGCGCY 1 cut(s) 321
BstHHI GCGC 2 cut(s) 225, 320
BstKTI GATC 2 cut(s) 576, 730
BstMBI GATC 2 cut(s) 573, 727
BstMWI GCNNNNNNNGC 4 cut(s) 119, 158, 474, 676
BstNI CCWGG 1 cut(s) 82
BstNSI RCATGY 1 cut(s) 508
BstSCI CCNGG 2 cut(s) 80, 622
BstSFI CTRYAG 1 cut(s) 674
BstV1I GCAGC 5 cut(s) 131, 160, 183, 383, 688
BstX2I RGATCY 1 cut(s) 573
BstYI RGATCY 1 cut(s) 573
BtsCI GGATG 1 cut(s) 53
BtsI GCAGTG 1 cut(s) 665
BtsIMutI CAGTG 2 cut(s) 293, 665
Cac8I GCNNGC 1 cut(s) 642
CaiI CAGNNNCTG 3 cut(s) 269, 377, 458
CfoI GCGC 2 cut(s) 225, 320
Cfr10I RCCGGY 1 cut(s) 601
Cfr13I GGNCC 1 cut(s) 661
CseI GACGC 1 cut(s) 608
CviAII CATG 6 cut(s) 23, 35, 277, 505, 595, 735
DdeI CTNAG 4 cut(s) 8, 217, 251, 314
DpnI GATC 2 cut(s) 575, 729
DpnII GATC 2 cut(s) 573, 727
Eco47I GGWCC 1 cut(s) 661
Eco47III AGCGCT 1 cut(s) 319
Eco57I CTGAAG 1 cut(s) 291
Eco88I CYCGRG 1 cut(s) 569
EcoRII CCWGG 1 cut(s) 80
FaeI CATG 6 cut(s) 26, 38, 280, 508, 598, 738
FalI AAGNNNNNCTT 1 cut(s) 714
FaqI GGGAC 2 cut(s) 529, 647
FatI CATG 6 cut(s) 22, 34, 276, 504, 594, 734
Fnu4HI GCNGC 5 cut(s) 120, 174, 197, 372, 677
FokI GGATG 1 cut(s) 40
Fsp4HI GCNGC 5 cut(s) 120, 174, 197, 372, 677
FspBI CTAG 1 cut(s) 288
GlaI GCGC 2 cut(s) 224, 319
GluI GCNGC 5 cut(s) 120, 174, 197, 372, 677
HaeII RGCGCY 1 cut(s) 321
HapII CCGG 2 cut(s) 602, 623
HgaI GACGC 1 cut(s) 608
HhaI GCGC 2 cut(s) 225, 320
Hin1II CATG 6 cut(s) 26, 38, 280, 508, 598, 738
Hin6I GCGC 2 cut(s) 223, 318
HinP1I GCGC 2 cut(s) 223, 318
HindIII AAGCTT 1 cut(s) 347
HinfI GANTC 4 cut(s) 143, 455, 524, 530
HpaII CCGG 2 cut(s) 602, 623
HphI GGTGA 1 cut(s) 556
Hpy166II GTNNAC 1 cut(s) 397
Hpy188I TCNGA 4 cut(s) 142, 454, 529, 721
Hpy188III TCNNGA 2 cut(s) 264, 571
Hpy8I GTNNAC 1 cut(s) 397
HpyAV CCTTC 2 cut(s) 82, 175
HpyCH4V TGCA 6 cut(s) 152, 176, 336, 438, 640, 676
HpyF10VI GCNNNNNNNGC 4 cut(s) 119, 158, 474, 676
HpyF3I CTNAG 4 cut(s) 8, 217, 251, 314
Hsp92II CATG 6 cut(s) 26, 38, 280, 508, 598, 738
HspAI GCGC 2 cut(s) 223, 318
Kzo9I GATC 2 cut(s) 573, 727
Lsp1109I GCAGC 5 cut(s) 131, 160, 183, 383, 688
LweI GCATC 3 cut(s) 417, 588, 627
MaeI CTAG 1 cut(s) 288
MaeIII GTNAC 2 cut(s) 3, 212
MalI GATC 2 cut(s) 575, 729
MboI GATC 2 cut(s) 573, 727
MboII GAAGA 4 cut(s) 64, 97, 722, 734
MflI RGATCY 1 cut(s) 573
MhlI GDGCHC 1 cut(s) 295
MluCI AATT 5 cut(s) 163, 189, 229, 301, 361
MlyI GAGTC 1 cut(s) 518
MnlI CCTC 3 cut(s) 403, 439, 578
MseI TTAA 5 cut(s) 192, 351, 389, 557, 632
MspA1I CMGCKG 1 cut(s) 611
MspI CCGG 2 cut(s) 602, 623
MspR9I CCNGG 2 cut(s) 82, 624
Mva1269I GAATGC 1 cut(s) 715
MvaI CCWGG 1 cut(s) 82
MwoI GCNNNNNNNGC 4 cut(s) 119, 158, 474, 676
NciI CCSGG 1 cut(s) 624
NdeII GATC 2 cut(s) 573, 727
NlaIII CATG 6 cut(s) 26, 38, 280, 508, 598, 738
NlaIV GGNNCC 1 cut(s) 663
NmuCI GTSAC 1 cut(s) 3
NspI RCATGY 1 cut(s) 508
PaeR7I CTCGAG 1 cut(s) 569
PciI ACATGT 1 cut(s) 504
PctI GAATGC 1 cut(s) 715
PfeI GAWTC 3 cut(s) 143, 455, 530
PfoI TCCNGGA 1 cut(s) 622
PkrI GCNGC 5 cut(s) 121, 175, 198, 373, 678
PleI GAGTC 1 cut(s) 518
PpsI GAGTC 1 cut(s) 518
PscI ACATGT 1 cut(s) 504
Psp6I CCWGG 1 cut(s) 80
PspGI CCWGG 1 cut(s) 80
PspN4I GGNNCC 1 cut(s) 663
PspPI GGNCC 1 cut(s) 661
PstI CTGCAG 1 cut(s) 678
PstNI CAGNNNCTG 3 cut(s) 269, 377, 458
PsuI RGATCY 1 cut(s) 573
PvuII CAGCTG 1 cut(s) 611
SaqAI TTAA 5 cut(s) 192, 351, 389, 557, 632
SatI GCNGC 5 cut(s) 120, 174, 197, 372, 677
Sau3AI GATC 2 cut(s) 573, 727
Sau96I GGNCC 1 cut(s) 661
SchI GAGTC 1 cut(s) 518
ScrFI CCNGG 2 cut(s) 82, 624
SduI GDGCHC 1 cut(s) 295
SetI ASST 6 cut(s) 93, 115, 198, 335, 351, 613
SfaNI GCATC 3 cut(s) 417, 588, 627
SfcI CTRYAG 1 cut(s) 674
Sfr274I CTCGAG 1 cut(s) 569
SinI GGWCC 1 cut(s) 661
SlaI CTCGAG 1 cut(s) 569
SmlI CTYRAG 1 cut(s) 569
SmoI CTYRAG 1 cut(s) 569
Sse9I AATT 5 cut(s) 163, 189, 229, 301, 361
SspMI CTAG 1 cut(s) 288
StyD4I CCNGG 2 cut(s) 80, 622
TaqI TCGA 1 cut(s) 570
TasI AATT 5 cut(s) 163, 189, 229, 301, 361
TfiI GAWTC 3 cut(s) 143, 455, 530
Tru1I TTAA 5 cut(s) 192, 351, 389, 557, 632
Tru9I TTAA 5 cut(s) 192, 351, 389, 557, 632
TscAI CASTG 2 cut(s) 300, 672
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 5 cut(s) 119, 173, 196, 371, 676
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 3 cut(s) 36, 39, 698
TspRI CASTG 2 cut(s) 300, 672
VpaK11BI GGWCC 1 cut(s) 661
XapI RAATTY 4 cut(s) 163, 229, 301, 361
XceI RCATGY 1 cut(s) 508
XcmI CCANNNNNNNNNTGG 1 cut(s) 130
XhoI CTCGAG 1 cut(s) 569
XspI CTAG 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.