Rh4DG155400

Protein of unknown function (DUF1997)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0009176
Physical Location & Seq
Reverse (-)
31039652 .. 31041782
2131 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG155400.1

Sequence Viewer

Length: 918 bp
ATGGTCTCCTGCCAACCCAATGGCATTTGCGTAATATTTCACAAACAAAACTGCATAGCTGAAAAAAAAGAAGGAACTTTTAAAGAACCAAATTCTGCAAAGATTATAATCATGTCACTAAGTATCAAGTGGCATGAACAACTACATGGCTTTACTTCATTCCCATCCCCTGCTACTCCAATTTTCAATACCAGGAAGAAAGCTGAAGTGATAAGAGTATTAGCTAAAGCAGCCAACTTGGGTTCTGAATCAAATGCAAAGAAAGCAAATTTGTGTGCTGCAAAGAAGGAAAGAATTAAGCTGCCAAGTTTTGATGGTAACTTAGGGCGCAAGAATTTTCACATTAGAGAGTTTCTAAGCCACCCATCAGGAACTGAAGCCATGCTCAATACTAGAGCACTGGAAAATTTTGAACCCCTTAGCACTGATACTTACAGGTGCACTTTGCCAAAGCTTAAACTTTTGAATTTTGAAGCAGCCCCTGTGGTGGATTTAAGAGTGAACCCAACCAATGAGGATTGTATGGTTGAGATGCTCTCTTGCAAGTTTGAGGGTTCAGAATCTGTGGAACGCCAAAACAGCCGTTTTTCAGCATTGATGACAAATCACATGTCGTGGGGGACAGACAACTCTGAATCATTTTTGGAAGTTGATGTGAAGTTAAATCTCACCCTCGAGATCTATACGCTCCCTTTTACCATGATGCCGGTATCAGCTGTTGAGCGTCCGGGAAACTTAATGATGCAAGCATTACTGGATAGGCTGAAGATGATCTTCCATGAAATGGTCCATTTCTGCATTGTTAAGATGACCTTTGGTAAAACCAAAATAGACAGCCGTGGCATTACTCTAGCCAATTTATCTCTGATTCCCATTGCTTCACGTGTTGGTTTTCTTGTAAGTTCCCGCTTCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1997

Protein Analysis

305

Amino Acids

34.3

Weight (kDa)

8.97

Isoelectric Point (pI)

33.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1997 PF09366 115 - 261 3.7e-31 Protein of unknown function (DUF1997)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 107
AccB7I CCANNNNNTGG 1 cut(s) 784
AciI CCGC 1 cut(s) 907
AcsI RAATTY 5 cut(s) 91, 268, 334, 406, 466
AcuI CTGAAG 3 cut(s) 225, 396, 785
AcvI CACGTG 1 cut(s) 884
AfiI CCNNNNNNNGG 2 cut(s) 487, 784
AflIII ACRYGT 2 cut(s) 609, 883
AgsI TTSAA 4 cut(s) 187, 413, 466, 473
AjnI CCWGG 1 cut(s) 191
AluBI AGCT 6 cut(s) 59, 203, 224, 301, 454, 716
AluI AGCT 6 cut(s) 59, 203, 224, 301, 454, 716
Alw21I GWGCWC 2 cut(s) 400, 443
Alw26I GTCTC 1 cut(s) 10
Alw44I GTGCAC 1 cut(s) 439
AlwNI CAGNNNCTG 3 cut(s) 374, 482, 563
Ama87I CYCGRG 1 cut(s) 674
ApaLI GTGCAC 1 cut(s) 439
ApeKI GCWGC 4 cut(s) 230, 278, 301, 476
ApoI RAATTY 5 cut(s) 91, 268, 334, 406, 466
ArsI GACNNNNNNTTYG 2 cut(s) 596, 628
AspLEI GCGC 1 cut(s) 330
AspS9I GGNCC 1 cut(s) 787
AsuC2I CCSGG 1 cut(s) 729
AsuHPI GGTGA 1 cut(s) 661
AvaI CYCGRG 1 cut(s) 674
AvaII GGWCC 1 cut(s) 787
BaeGI GKGCMC 1 cut(s) 443
BbrPI CACGTG 1 cut(s) 884
Bbv12I GWGCWC 2 cut(s) 400, 443
BbvI GCAGC 4 cut(s) 242, 265, 288, 488
BccI CCATC 3 cut(s) 172, 308, 373
BceAI ACGGC 2 cut(s) 567, 822
BciT130I CCWGG 1 cut(s) 193
BcnI CCSGG 1 cut(s) 729
BcoDI GTCTC 1 cut(s) 10
BfaI CTAG 2 cut(s) 393, 851
BglII AGATCT 1 cut(s) 678
BisI GCNGC 4 cut(s) 231, 279, 302, 477
BlsI GCNGC 4 cut(s) 232, 280, 303, 478
Bme1390I CCNGG 2 cut(s) 193, 729
Bme18I GGWCC 1 cut(s) 787
BmeT110I CYCGRG 1 cut(s) 674
BmgT120I GGNCC 1 cut(s) 787
BmrFI CCNGG 2 cut(s) 193, 729
BmsI GCATC 3 cut(s) 522, 693, 732
BplI GAGNNNNNCTC 2 cut(s) 521, 553
Bpu10I CCTNAGC 1 cut(s) 419
BpuMI CCSGG 1 cut(s) 729
BsaAI YACGTR 1 cut(s) 884
BsaBI GATNNNNATC 1 cut(s) 107
BsaI GGTCTC 1 cut(s) 10
BsaJI CCNNGG 1 cut(s) 838
Bsc4I CCNNNNNNNGG 2 cut(s) 487, 784
Bse118I RCCGGY 1 cut(s) 706
Bse1I ACTGG 2 cut(s) 405, 759
Bse3DI GCAATG 1 cut(s) 873
Bse8I GATNNNNATC 1 cut(s) 107
BseBI CCWGG 1 cut(s) 193
BseDI CCNNGG 1 cut(s) 838
BseGI GGATG 1 cut(s) 164
BseJI GATNNNNATC 1 cut(s) 107
BseLI CCNNNNNNNGG 2 cut(s) 487, 784
BseMI GCAATG 1 cut(s) 873
BseNI ACTGG 2 cut(s) 405, 759
BseSI GKGCMC 1 cut(s) 443
BseXI GCAGC 4 cut(s) 242, 265, 288, 488
BsiHKAI GWGCWC 2 cut(s) 400, 443
BsiHKCI CYCGRG 1 cut(s) 674
BsiSI CCGG 2 cut(s) 707, 728
BslFI GGGAC 1 cut(s) 634
BslI CCNNNNNNNGG 2 cut(s) 487, 784
BsmAI GTCTC 1 cut(s) 10
BsmFI GGGAC 1 cut(s) 634
Bso31I GGTCTC 1 cut(s) 10
BsoBI CYCGRG 1 cut(s) 674
Bsp1286I GDGCHC 2 cut(s) 400, 443
Bsp143I GATC 2 cut(s) 678, 771
BspACI CCGC 1 cut(s) 907
BspTNI GGTCTC 1 cut(s) 10
BsrDI GCAATG 1 cut(s) 873
BsrFI RCCGGY 1 cut(s) 706
BsrI ACTGG 2 cut(s) 405, 759
BssAI RCCGGY 1 cut(s) 706
BssECI CCNNGG 1 cut(s) 838
BssMI GATC 2 cut(s) 678, 771
Bst2UI CCWGG 1 cut(s) 193
BstBAI YACGTR 1 cut(s) 884
BstC8I GCNNGC 1 cut(s) 747
BstDEI CTNAG 4 cut(s) 119, 322, 356, 419
BstDSI CCRYGG 1 cut(s) 838
BstF5I GGATG 1 cut(s) 164
BstHHI GCGC 1 cut(s) 330
BstKTI GATC 2 cut(s) 681, 774
BstMAI GTCTC 1 cut(s) 10
BstMBI GATC 2 cut(s) 678, 771
BstMWI GCNNNNNNNGC 3 cut(s) 230, 263, 579
BstNI CCWGG 1 cut(s) 193
BstNSI RCATGY 1 cut(s) 613
BstSCI CCNGG 2 cut(s) 191, 727
BstSLI GKGCMC 1 cut(s) 443
BstV1I GCAGC 4 cut(s) 242, 265, 288, 488
BstX2I RGATCY 1 cut(s) 678
BstXI CCANNNNNNTGG 1 cut(s) 20
BstYI RGATCY 1 cut(s) 678
BtgI CCRYGG 1 cut(s) 838
BtsCI GGATG 1 cut(s) 164
BtsIMutI CAGTG 2 cut(s) 398, 423
Cac8I GCNNGC 1 cut(s) 747
CaiI CAGNNNCTG 3 cut(s) 374, 482, 563
CfoI GCGC 1 cut(s) 330
Cfr10I RCCGGY 1 cut(s) 706
Cfr13I GGNCC 1 cut(s) 787
CseI GACGC 1 cut(s) 713
CviAII CATG 7 cut(s) 112, 134, 146, 382, 610, 700, 779
DdeI CTNAG 4 cut(s) 119, 322, 356, 419
DpnI GATC 2 cut(s) 680, 773
DpnII GATC 2 cut(s) 678, 771
DraI TTTAAA 1 cut(s) 82
Eco31I GGTCTC 1 cut(s) 10
Eco47I GGWCC 1 cut(s) 787
Eco57I CTGAAG 3 cut(s) 225, 396, 785
Eco72I CACGTG 1 cut(s) 884
Eco88I CYCGRG 1 cut(s) 674
EcoRII CCWGG 1 cut(s) 191
FaeI CATG 7 cut(s) 115, 137, 149, 385, 613, 703, 782
FalI AAGNNNNNCTT 4 cut(s) 758, 790, 797, 829
FaqI GGGAC 1 cut(s) 634
FatI CATG 7 cut(s) 111, 133, 145, 381, 609, 699, 778
FauI CCCGC 1 cut(s) 914
Fnu4HI GCNGC 4 cut(s) 231, 279, 302, 477
FokI GGATG 1 cut(s) 151
Fsp4HI GCNGC 4 cut(s) 231, 279, 302, 477
FspBI CTAG 2 cut(s) 393, 851
GlaI GCGC 1 cut(s) 329
GluI GCNGC 4 cut(s) 231, 279, 302, 477
HapII CCGG 2 cut(s) 707, 728
HgaI GACGC 1 cut(s) 713
HhaI GCGC 1 cut(s) 330
Hin1II CATG 7 cut(s) 115, 137, 149, 385, 613, 703, 782
Hin6I GCGC 1 cut(s) 328
HinP1I GCGC 1 cut(s) 328
HindIII AAGCTT 1 cut(s) 452
HinfI GANTC 4 cut(s) 248, 560, 635, 868
HpaII CCGG 2 cut(s) 707, 728
HphI GGTGA 1 cut(s) 661
Hpy166II GTNNAC 2 cut(s) 441, 502
Hpy188I TCNGA 4 cut(s) 247, 559, 634, 867
Hpy188III TCNNGA 2 cut(s) 369, 676
Hpy8I GTNNAC 2 cut(s) 441, 502
HpyAV CCTTC 2 cut(s) 65, 280
HpyCH4IV ACGT 1 cut(s) 883
HpyCH4V TGCA 8 cut(s) 54, 98, 257, 281, 441, 543, 745, 798
HpyF10VI GCNNNNNNNGC 3 cut(s) 230, 263, 579
HpyF3I CTNAG 4 cut(s) 119, 322, 356, 419
HpySE526I ACGT 1 cut(s) 883
Hsp92II CATG 7 cut(s) 115, 137, 149, 385, 613, 703, 782
HspAI GCGC 1 cut(s) 328
Kzo9I GATC 2 cut(s) 678, 771
LmnI GCTCC 1 cut(s) 693
Lsp1109I GCAGC 4 cut(s) 242, 265, 288, 488
LweI GCATC 3 cut(s) 522, 693, 732
MaeI CTAG 2 cut(s) 393, 851
MaeII ACGT 1 cut(s) 883
MaeIII GTNAC 2 cut(s) 114, 317
MalI GATC 2 cut(s) 680, 773
MboI GATC 2 cut(s) 678, 771
MboII GAAGA 3 cut(s) 208, 766, 778
MflI RGATCY 1 cut(s) 678
MhlI GDGCHC 2 cut(s) 400, 443
MluCI AATT 8 cut(s) 91, 180, 268, 294, 334, 406, 466, 856
MnlI CCTC 3 cut(s) 508, 544, 683
MseI TTAA 7 cut(s) 81, 297, 456, 494, 662, 737, 804
MspA1I CMGCKG 1 cut(s) 716
MspI CCGG 2 cut(s) 707, 728
MspR9I CCNGG 2 cut(s) 193, 729
MvaI CCWGG 1 cut(s) 193
MwoI GCNNNNNNNGC 3 cut(s) 230, 263, 579
NciI CCSGG 1 cut(s) 729
NdeII GATC 2 cut(s) 678, 771
NlaIII CATG 7 cut(s) 115, 137, 149, 385, 613, 703, 782
NmuCI GTSAC 1 cut(s) 114
NspI RCATGY 1 cut(s) 613
PaeR7I CTCGAG 1 cut(s) 674
PciI ACATGT 1 cut(s) 609
PfeI GAWTC 4 cut(s) 248, 560, 635, 868
PflMI CCANNNNNTGG 1 cut(s) 784
PfoI TCCNGGA 1 cut(s) 727
PkrI GCNGC 4 cut(s) 232, 280, 303, 478
PmaCI CACGTG 1 cut(s) 884
PmlI CACGTG 1 cut(s) 884
Ppu21I YACGTR 1 cut(s) 884
PscI ACATGT 1 cut(s) 609
PsiI TTATAA 1 cut(s) 107
Psp6I CCWGG 1 cut(s) 191
PspCI CACGTG 1 cut(s) 884
PspGI CCWGG 1 cut(s) 191
PspPI GGNCC 1 cut(s) 787
PstNI CAGNNNCTG 3 cut(s) 374, 482, 563
PsuI RGATCY 1 cut(s) 678
PvuII CAGCTG 1 cut(s) 716
SaqAI TTAA 7 cut(s) 81, 297, 456, 494, 662, 737, 804
SatI GCNGC 4 cut(s) 231, 279, 302, 477
Sau3AI GATC 2 cut(s) 678, 771
Sau96I GGNCC 1 cut(s) 787
ScrFI CCNGG 2 cut(s) 193, 729
SduI GDGCHC 2 cut(s) 400, 443
SetI ASST 9 cut(s) 61, 205, 226, 303, 440, 456, 718, 815, 886
SfaNI GCATC 3 cut(s) 522, 693, 732
Sfr274I CTCGAG 1 cut(s) 674
SinI GGWCC 1 cut(s) 787
SlaI CTCGAG 1 cut(s) 674
SmlI CTYRAG 1 cut(s) 674
SmoI CTYRAG 1 cut(s) 674
Sse9I AATT 8 cut(s) 91, 180, 268, 294, 334, 406, 466, 856
SsiI CCGC 1 cut(s) 907
SspI AATATT 1 cut(s) 36
SspMI CTAG 2 cut(s) 393, 851
StyD4I CCNGG 2 cut(s) 191, 727
TaiI ACGT 1 cut(s) 886
TaqI TCGA 2 cut(s) 675, 912
TasI AATT 8 cut(s) 91, 180, 268, 294, 334, 406, 466, 856
TfiI GAWTC 4 cut(s) 248, 560, 635, 868
Tru1I TTAA 7 cut(s) 81, 297, 456, 494, 662, 737, 804
Tru9I TTAA 7 cut(s) 81, 297, 456, 494, 662, 737, 804
TscAI CASTG 2 cut(s) 405, 430
TseFI GTSAC 1 cut(s) 114
TseI GCWGC 4 cut(s) 230, 278, 301, 476
Tsp45I GTSAC 1 cut(s) 114
TspDTI ATGAA 3 cut(s) 147, 150, 795
TspRI CASTG 2 cut(s) 405, 430
Van91I CCANNNNNTGG 1 cut(s) 784
VneI GTGCAC 1 cut(s) 439
VpaK11BI GGWCC 1 cut(s) 787
XapI RAATTY 5 cut(s) 91, 268, 334, 406, 466
XceI RCATGY 1 cut(s) 613
XhoI CTCGAG 1 cut(s) 674
XspI CTAG 2 cut(s) 393, 851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.