Rw4G013380

Protein of unknown function (DUF1997)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0009176
Physical Location & Seq
Reverse (-)
31274253 .. 31279317
5065 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G013380.1

Sequence Viewer

Length: 729 bp
ATGTTGCTTATTGGTGGGATCAGTTGCAGAGCTCTCTGGCATGAACAACTACATGGCTTTACTTCATTCCCATCCCCTGCTACTCCAATTTTCAATACCAGGAAGAAAGTTGAAGTGATAAGAGTATTAGCTAAAGCAGCCAACTTGGGTTCTGAATCAAATGCAAAGAAAGCAAATTTGTGTGCTGCAAAGAAGGAAAGAATTAAGCTGCCAAGTTTTGATGGTAACTTAGGGCGCAAGAATTTTCACATTAGAGAGTTTCTAAGCCACCCATCAGGAACTGAAGCCATGCTCAATACTAGAGCACTGGAAAATTTTGAACCCCTTAGCACTGATACTTACAGGTGCACTTTGCCAAAGCTTAAACTTTTGAATTTTGAAGCAGCCCCTGTGGTGGATTTAAGAGTGAACCCAACCAATGAGGATTGTATGGTTGAGATGCTCTCTTGCAAGTTTGAGGGTTCAGAATCTGTGGAACGCCAAAACAGCCGTTTTTCAGCATTGATGACAAATCACATGTCGTGGGGGACAGACAACTCTGAATCATTTTTGGAAGTAGATGTGAAGTTAAATCTCACCCTCGAGATCTATACGCCCCCTTTTACCCTGATGCCGGTATCAGCTGTTGAGCGTCCGGGAAACTTAATGATGCAAGCATTACTGGATAGGCTGGTCCCACTGCTTCTGCAGCAACTCCTACAAGATTATGACAAATGGGTTCATAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1997

Protein Analysis

242

Amino Acids

27.34

Weight (kDa)

7.67

Isoelectric Point (pI)

40.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1997 PF09366 84 - 240 1.3e-38 Protein of unknown function (DUF1997)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 26
AcsI RAATTY 4 cut(s) 175, 241, 313, 373
AcuI CTGAAG 1 cut(s) 303
AfiI CCNNNNNNNGG 2 cut(s) 394, 613
AflIII ACRYGT 1 cut(s) 516
AgsI TTSAA 5 cut(s) 94, 113, 320, 373, 380
AjnI CCWGG 1 cut(s) 98
AluBI AGCT 5 cut(s) 32, 131, 208, 361, 623
AluI AGCT 5 cut(s) 32, 131, 208, 361, 623
Alw21I GWGCWC 3 cut(s) 34, 307, 350
Alw44I GTGCAC 1 cut(s) 346
AlwI GGATC 1 cut(s) 26
AlwNI CAGNNNCTG 3 cut(s) 281, 389, 470
Ama87I CYCGRG 1 cut(s) 581
ApaLI GTGCAC 1 cut(s) 346
ApeKI GCWGC 5 cut(s) 137, 185, 208, 383, 688
ApoI RAATTY 4 cut(s) 175, 241, 313, 373
ArsI GACNNNNNNTTYG 2 cut(s) 503, 535
AspLEI GCGC 1 cut(s) 237
AspS9I GGNCC 1 cut(s) 673
AsuC2I CCSGG 1 cut(s) 636
AsuHPI GGTGA 1 cut(s) 568
AvaI CYCGRG 1 cut(s) 581
AvaII GGWCC 1 cut(s) 673
BaeGI GKGCMC 1 cut(s) 350
BanII GRGCYC 1 cut(s) 34
Bbv12I GWGCWC 3 cut(s) 34, 307, 350
BbvI GCAGC 5 cut(s) 149, 172, 195, 395, 700
BccI CCATC 3 cut(s) 79, 215, 280
BceAI ACGGC 1 cut(s) 474
BciT130I CCWGG 1 cut(s) 100
BcnI CCSGG 1 cut(s) 636
BfaI CTAG 1 cut(s) 300
BfmI CTRYAG 1 cut(s) 686
BglII AGATCT 1 cut(s) 585
BisI GCNGC 5 cut(s) 138, 186, 209, 384, 689
BlsI GCNGC 5 cut(s) 139, 187, 210, 385, 690
Bme1390I CCNGG 2 cut(s) 100, 636
Bme18I GGWCC 1 cut(s) 673
BmeT110I CYCGRG 1 cut(s) 581
BmgT120I GGNCC 1 cut(s) 673
BmiI GGNNCC 1 cut(s) 675
BmrFI CCNGG 2 cut(s) 100, 636
BmsI GCATC 3 cut(s) 429, 600, 639
BplI GAGNNNNNCTC 2 cut(s) 428, 460
Bpu10I CCTNAGC 1 cut(s) 326
BpuMI CCSGG 1 cut(s) 636
Bsc4I CCNNNNNNNGG 2 cut(s) 394, 613
Bse118I RCCGGY 1 cut(s) 613
Bse1I ACTGG 2 cut(s) 312, 666
BseBI CCWGG 1 cut(s) 100
BseGI GGATG 1 cut(s) 71
BseLI CCNNNNNNNGG 2 cut(s) 394, 613
BseNI ACTGG 2 cut(s) 312, 666
BseSI GKGCMC 1 cut(s) 350
BseXI GCAGC 5 cut(s) 149, 172, 195, 395, 700
BsiHKAI GWGCWC 3 cut(s) 34, 307, 350
BsiHKCI CYCGRG 1 cut(s) 581
BsiSI CCGG 2 cut(s) 614, 635
BslFI GGGAC 2 cut(s) 541, 659
BslI CCNNNNNNNGG 2 cut(s) 394, 613
BsmFI GGGAC 2 cut(s) 541, 659
BsoBI CYCGRG 1 cut(s) 581
Bsp1286I GDGCHC 3 cut(s) 34, 307, 350
Bsp143I GATC 2 cut(s) 18, 585
BspLI GGNNCC 1 cut(s) 675
BspMAI CTGCAG 1 cut(s) 690
BspPI GGATC 1 cut(s) 26
BsrFI RCCGGY 1 cut(s) 613
BsrI ACTGG 2 cut(s) 312, 666
BssAI RCCGGY 1 cut(s) 613
BssMI GATC 2 cut(s) 18, 585
Bst2UI CCWGG 1 cut(s) 100
BstC8I GCNNGC 1 cut(s) 654
BstDEI CTNAG 3 cut(s) 229, 263, 326
BstF5I GGATG 1 cut(s) 71
BstHHI GCGC 1 cut(s) 237
BstKTI GATC 2 cut(s) 21, 588
BstMBI GATC 2 cut(s) 18, 585
BstMWI GCNNNNNNNGC 4 cut(s) 137, 170, 486, 688
BstNI CCWGG 1 cut(s) 100
BstNSI RCATGY 1 cut(s) 520
BstSCI CCNGG 2 cut(s) 98, 634
BstSFI CTRYAG 1 cut(s) 686
BstSLI GKGCMC 1 cut(s) 350
BstV1I GCAGC 5 cut(s) 149, 172, 195, 395, 700
BstX2I RGATCY 1 cut(s) 585
BstYI RGATCY 1 cut(s) 585
BtsCI GGATG 1 cut(s) 71
BtsI GCAGTG 1 cut(s) 677
BtsIMutI CAGTG 3 cut(s) 305, 330, 677
Cac8I GCNNGC 1 cut(s) 654
CaiI CAGNNNCTG 3 cut(s) 281, 389, 470
CfoI GCGC 1 cut(s) 237
Cfr10I RCCGGY 1 cut(s) 613
Cfr13I GGNCC 1 cut(s) 673
CseI GACGC 1 cut(s) 620
CviAII CATG 4 cut(s) 41, 53, 289, 517
DdeI CTNAG 3 cut(s) 229, 263, 326
DpnI GATC 2 cut(s) 20, 587
DpnII GATC 2 cut(s) 18, 585
Ecl136II GAGCTC 1 cut(s) 32
Eco24I GRGCYC 1 cut(s) 34
Eco47I GGWCC 1 cut(s) 673
Eco53kI GAGCTC 1 cut(s) 32
Eco57I CTGAAG 1 cut(s) 303
Eco88I CYCGRG 1 cut(s) 581
EcoICRI GAGCTC 1 cut(s) 32
EcoRII CCWGG 1 cut(s) 98
EcoT38I GRGCYC 1 cut(s) 34
FaeI CATG 4 cut(s) 44, 56, 292, 520
FaiI YATR 8 cut(s) 42, 54, 290, 431, 518, 591, 708, 723
FaqI GGGAC 2 cut(s) 541, 659
FatI CATG 4 cut(s) 40, 52, 288, 516
Fnu4HI GCNGC 5 cut(s) 138, 186, 209, 384, 689
FokI GGATG 1 cut(s) 58
FriOI GRGCYC 1 cut(s) 34
Fsp4HI GCNGC 5 cut(s) 138, 186, 209, 384, 689
FspBI CTAG 1 cut(s) 300
GlaI GCGC 1 cut(s) 236
GluI GCNGC 5 cut(s) 138, 186, 209, 384, 689
HapII CCGG 2 cut(s) 614, 635
HgaI GACGC 1 cut(s) 620
HhaI GCGC 1 cut(s) 237
Hin1II CATG 4 cut(s) 44, 56, 292, 520
Hin6I GCGC 1 cut(s) 235
HinP1I GCGC 1 cut(s) 235
HindIII AAGCTT 1 cut(s) 359
HinfI GANTC 3 cut(s) 155, 467, 542
HpaII CCGG 2 cut(s) 614, 635
HphI GGTGA 1 cut(s) 568
Hpy166II GTNNAC 2 cut(s) 348, 409
Hpy188I TCNGA 3 cut(s) 154, 466, 541
Hpy188III TCNNGA 2 cut(s) 276, 583
Hpy8I GTNNAC 2 cut(s) 348, 409
HpyAV CCTTC 1 cut(s) 187
HpyCH4V TGCA 7 cut(s) 27, 164, 188, 348, 450, 652, 688
HpyF10VI GCNNNNNNNGC 4 cut(s) 137, 170, 486, 688
HpyF3I CTNAG 3 cut(s) 229, 263, 326
Hsp92II CATG 4 cut(s) 44, 56, 292, 520
HspAI GCGC 1 cut(s) 235
Kzo9I GATC 2 cut(s) 18, 585
Lsp1109I GCAGC 5 cut(s) 149, 172, 195, 395, 700
LweI GCATC 3 cut(s) 429, 600, 639
MaeI CTAG 1 cut(s) 300
MaeIII GTNAC 1 cut(s) 224
MalI GATC 2 cut(s) 20, 587
MboI GATC 2 cut(s) 18, 585
MboII GAAGA 1 cut(s) 115
MflI RGATCY 1 cut(s) 585
MhlI GDGCHC 3 cut(s) 34, 307, 350
MluCI AATT 6 cut(s) 87, 175, 201, 241, 313, 373
MnlI CCTC 3 cut(s) 415, 451, 590
MseI TTAA 5 cut(s) 204, 363, 401, 569, 644
MspA1I CMGCKG 1 cut(s) 623
MspI CCGG 2 cut(s) 614, 635
MspR9I CCNGG 2 cut(s) 100, 636
MvaI CCWGG 1 cut(s) 100
MwoI GCNNNNNNNGC 4 cut(s) 137, 170, 486, 688
NciI CCSGG 1 cut(s) 636
NdeII GATC 2 cut(s) 18, 585
NlaIII CATG 4 cut(s) 44, 56, 292, 520
NlaIV GGNNCC 1 cut(s) 675
NspI RCATGY 1 cut(s) 520
PaeR7I CTCGAG 1 cut(s) 581
PciI ACATGT 1 cut(s) 516
PfeI GAWTC 3 cut(s) 155, 467, 542
PfoI TCCNGGA 1 cut(s) 634
PkrI GCNGC 5 cut(s) 139, 187, 210, 385, 690
PscI ACATGT 1 cut(s) 516
Psp124BI GAGCTC 1 cut(s) 34
Psp6I CCWGG 1 cut(s) 98
PspGI CCWGG 1 cut(s) 98
PspN4I GGNNCC 1 cut(s) 675
PspPI GGNCC 1 cut(s) 673
PstI CTGCAG 1 cut(s) 690
PstNI CAGNNNCTG 3 cut(s) 281, 389, 470
PsuI RGATCY 1 cut(s) 585
PvuII CAGCTG 1 cut(s) 623
SacI GAGCTC 1 cut(s) 34
SaqAI TTAA 5 cut(s) 204, 363, 401, 569, 644
SatI GCNGC 5 cut(s) 138, 186, 209, 384, 689
Sau3AI GATC 2 cut(s) 18, 585
Sau96I GGNCC 1 cut(s) 673
ScrFI CCNGG 2 cut(s) 100, 636
SduI GDGCHC 3 cut(s) 34, 307, 350
SetI ASST 6 cut(s) 34, 133, 210, 347, 363, 625
SfaNI GCATC 3 cut(s) 429, 600, 639
SfcI CTRYAG 1 cut(s) 686
Sfr274I CTCGAG 1 cut(s) 581
SinI GGWCC 1 cut(s) 673
SlaI CTCGAG 1 cut(s) 581
SmlI CTYRAG 1 cut(s) 581
SmoI CTYRAG 1 cut(s) 581
Sse9I AATT 6 cut(s) 87, 175, 201, 241, 313, 373
SspMI CTAG 1 cut(s) 300
SstI GAGCTC 1 cut(s) 34
StyD4I CCNGG 2 cut(s) 98, 634
TaqI TCGA 1 cut(s) 582
TasI AATT 6 cut(s) 87, 175, 201, 241, 313, 373
TfiI GAWTC 3 cut(s) 155, 467, 542
Tru1I TTAA 5 cut(s) 204, 363, 401, 569, 644
Tru9I TTAA 5 cut(s) 204, 363, 401, 569, 644
TscAI CASTG 3 cut(s) 312, 337, 684
TseI GCWGC 5 cut(s) 137, 185, 208, 383, 688
TspDTI ATGAA 3 cut(s) 54, 57, 710
TspRI CASTG 3 cut(s) 312, 337, 684
VneI GTGCAC 1 cut(s) 346
VpaK11BI GGWCC 1 cut(s) 673
XapI RAATTY 4 cut(s) 175, 241, 313, 373
XceI RCATGY 1 cut(s) 520
XhoI CTCGAG 1 cut(s) 581
XspI CTAG 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.