MD08G1134200.v1.1

selenoprotein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0014423
Physical Location & Seq
Forward (+)
12786010 .. 12790197
4188 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1134200.v1.1.491

Sequence Viewer

Length: 669 bp
ATGGCGCCGAGGAAACGCAAGACTACTGAGCAAGAAGAAAAGCAGCTGGTGGCTCAGGACTCGGGTCAGCGAGTGACTCGGAGTATGGCTCGGCCGGCTCCGAGTGGGAGCTCGGCTGAGCCACGAGCCGTGGCGCCGGCGAGGAAGAAGGCCAAGGGAGCTCAGAAGAAGAAAGAGCCGGCGAAGAAGGAAGAGGTGGAGGTGGAAGAAGCGGAAGCGGAAGCTGTGAAGGAAGAGGAGGACAACTCCGGCGAGAGCAAGGCGGAGGCCGACGCGGAGAACGAGAACAAGAAGAAGAAAGGGAAGGAGAAAGTGGAGGCCGAGGTGGAGAACAAGGAGGAGAAAGGGAAAGCGAAAGTGGAGGACGACGGAGAAGGTACGGAGGCTGACGACGACGAGTCGGACAAGAGGACCATTGTGATTGAGCATTGCAAGCAATGCAAGTCATTCAAGGTAAGAGCTGATCAGGTCAAAAATGGTTTGGAGAAAGGTGTTCCTGGCATCAAAGTTCTACTAAACCCTGATAAGCCAAGAAAAGGATGCTTAGAGATACGGGAGGAAGGCGGCGAGACATTCCTCAGTCTTCTGGACATGAAACGACCATTTTCAAAAATGAAGGCGCTGAACATGGAGGAAGTCATTAACGATATAGTTGAAAAAATCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

223

Amino Acids

24.69

Weight (kDa)

5.89

Isoelectric Point (pI)

45.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014423)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24440 AT4G31360
fragaria_vesca FvH4_2g30710
malus_domestica MD00G1173100.v1.1 MD08G1134200.v1.1 MD15G1109600.v1.1
prunus_persica Prupe.1G462000_v2.0.a1
pyrus_communis pycom08g11310
rosa_chinensis RchiOBHm_Chr6g0312181
rosa_laevigata RLG00000010345
rosa_roxburghii Rroxscaffold_7G00156980
rosa_rugosa Rorug06G0393600
rosa_samantha Rh6AG510600 Rh6BG521700 Rh6CG527600 Rh6DG513000
rosa_wichuraiana Rw6G044410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 4, 133
AccII CGCG 1 cut(s) 275
AciI CCGC 5 cut(s) 212, 218, 263, 275, 564
AcoI YGGCCR 1 cut(s) 92
AcyI GRCGYC 2 cut(s) 5, 134
AfaI GTAC 1 cut(s) 379
AfiI CCNNNNNNNGG 1 cut(s) 536
AgsI TTSAA 3 cut(s) 451, 609, 656
AhdI GACNNNNNGTC 1 cut(s) 397
AjnI CCWGG 1 cut(s) 496
AluBI AGCT 5 cut(s) 46, 111, 161, 224, 461
AluI AGCT 5 cut(s) 46, 111, 161, 224, 461
Alw21I GWGCWC 2 cut(s) 113, 163
Alw26I GTCTC 1 cut(s) 563
Ama87I CYCGRG 1 cut(s) 61
AoxI GGCC 4 cut(s) 92, 150, 267, 318
ApeKI GCWGC 1 cut(s) 43
AspLEI GCGC 3 cut(s) 7, 136, 622
AspS9I GGNCC 1 cut(s) 411
AvaI CYCGRG 1 cut(s) 61
AvaII GGWCC 1 cut(s) 411
BanI GGYRCC 2 cut(s) 4, 133
BanII GRGCYC 2 cut(s) 113, 163
BauI CACGAG 1 cut(s) 123
BbsI GAAGAC 1 cut(s) 575
Bbv12I GWGCWC 2 cut(s) 113, 163
BbvI GCAGC 1 cut(s) 55
BceAI ACGGC 1 cut(s) 113
BciT130I CCWGG 1 cut(s) 498
BclI TGATCA 1 cut(s) 463
BcoDI GTCTC 1 cut(s) 563
BfoI RGCGCY 3 cut(s) 8, 137, 623
BisI GCNGC 2 cut(s) 44, 565
BlpI GCTNAGC 1 cut(s) 117
BlsI GCNGC 2 cut(s) 45, 566
Bme1390I CCNGG 1 cut(s) 498
Bme18I GGWCC 1 cut(s) 411
BmeRI GACNNNNNGTC 1 cut(s) 397
BmeT110I CYCGRG 1 cut(s) 61
BmgT120I GGNCC 1 cut(s) 411
BmiI GGNNCC 3 cut(s) 6, 99, 135
BmrFI CCNGG 1 cut(s) 498
BmsI GCATC 2 cut(s) 510, 530
BoxI GACNNNNGTC 1 cut(s) 63
BpiI GAAGAC 1 cut(s) 575
BplI GAGNNNNNCTC 4 cut(s) 73, 105, 230, 262
Bpu10I CCTNAGC 1 cut(s) 54
Bpu1102I GCTNAGC 1 cut(s) 117
BsaHI GRCGYC 2 cut(s) 5, 134
BsaJI CCNNGG 4 cut(s) 8, 129, 153, 321
Bsc4I CCNNNNNNNGG 1 cut(s) 536
Bse118I RCCGGY 3 cut(s) 94, 136, 178
Bse3DI GCAATG 2 cut(s) 427, 443
BseBI CCWGG 1 cut(s) 498
BseDI CCNNGG 4 cut(s) 8, 129, 153, 321
BseGI GGATG 1 cut(s) 545
BseLI CCNNNNNNNGG 1 cut(s) 536
BseMI GCAATG 2 cut(s) 427, 443
BseMII CTCAG 5 cut(s) 18, 68, 108, 176, 592
BseRI GAGGAG 2 cut(s) 251, 353
BseX3I CGGCCG 1 cut(s) 92
BseXI GCAGC 1 cut(s) 55
Bsh1236I CGCG 1 cut(s) 275
Bsh1285I CGRYCG 1 cut(s) 95
BshFI GGCC 4 cut(s) 94, 152, 269, 320
BshNI GGYRCC 2 cut(s) 4, 133
BsiEI CGRYCG 1 cut(s) 95
BsiHKAI GWGCWC 2 cut(s) 113, 163
BsiHKCI CYCGRG 1 cut(s) 61
BsiSI CCGG 4 cut(s) 95, 137, 179, 249
BslI CCNNNNNNNGG 1 cut(s) 536
BsmAI GTCTC 1 cut(s) 563
BsnI GGCC 4 cut(s) 94, 152, 269, 320
BsoBI CYCGRG 1 cut(s) 61
Bsp1286I GDGCHC 2 cut(s) 113, 163
Bsp143I GATC 1 cut(s) 463
Bsp1720I GCTNAGC 1 cut(s) 117
BspACI CCGC 5 cut(s) 212, 218, 263, 275, 564
BspANI GGCC 4 cut(s) 94, 152, 269, 320
BspCNI CTCAG 5 cut(s) 19, 67, 109, 175, 591
BspFNI CGCG 1 cut(s) 275
BspLI GGNNCC 3 cut(s) 6, 99, 135
BspT107I GGYRCC 2 cut(s) 4, 133
BsrDI GCAATG 2 cut(s) 427, 443
BsrFI RCCGGY 3 cut(s) 94, 136, 178
BssAI RCCGGY 3 cut(s) 94, 136, 178
BssECI CCNNGG 4 cut(s) 8, 129, 153, 321
BssMI GATC 1 cut(s) 463
BssNI GRCGYC 2 cut(s) 5, 134
BssSI CACGAG 1 cut(s) 123
BssT1I CCWWGG 1 cut(s) 153
Bst2BI CACGAG 1 cut(s) 123
Bst2UI CCWGG 1 cut(s) 498
Bst6I CTCTTC 2 cut(s) 186, 228
BstACI GRCGYC 2 cut(s) 5, 134
BstAPI GCANNNNNTGC 1 cut(s) 438
BstC8I GCNNGC 4 cut(s) 96, 138, 180, 434
BstDEI CTNAG 6 cut(s) 27, 54, 117, 162, 544, 578
BstDSI CCRYGG 1 cut(s) 129
BstF5I GGATG 1 cut(s) 545
BstFNI CGCG 1 cut(s) 275
BstH2I RGCGCY 3 cut(s) 8, 137, 623
BstHHI GCGC 3 cut(s) 7, 136, 622
BstKTI GATC 1 cut(s) 466
BstMAI GTCTC 1 cut(s) 563
BstMBI GATC 1 cut(s) 463
BstMCI CGRYCG 1 cut(s) 95
BstMWI GCNNNNNNNGC 4 cut(s) 95, 158, 433, 438
BstNI CCWGG 1 cut(s) 498
BstPAI GACNNNNGTC 1 cut(s) 63
BstSCI CCNGG 1 cut(s) 496
BstUI CGCG 1 cut(s) 275
BstV1I GCAGC 1 cut(s) 55
BstV2I GAAGAC 1 cut(s) 575
BstZI CGGCCG 1 cut(s) 92
BsuRI GGCC 4 cut(s) 94, 152, 269, 320
BtgI CCRYGG 1 cut(s) 129
BtsCI GGATG 1 cut(s) 545
Cac8I GCNNGC 4 cut(s) 96, 138, 180, 434
CfoI GCGC 3 cut(s) 7, 136, 622
Cfr10I RCCGGY 3 cut(s) 94, 136, 178
Cfr13I GGNCC 1 cut(s) 411
CseI GACGC 1 cut(s) 281
Csp6I GTAC 1 cut(s) 378
CviAII CATG 2 cut(s) 592, 628
CviQI GTAC 1 cut(s) 378
DdeI CTNAG 6 cut(s) 27, 54, 117, 162, 544, 578
DinI GGCGCC 2 cut(s) 6, 135
DpnI GATC 1 cut(s) 465
DpnII GATC 1 cut(s) 463
DriI GACNNNNNGTC 1 cut(s) 397
EaeI YGGCCR 1 cut(s) 92
EagI CGGCCG 1 cut(s) 92
Eam1104I CTCTTC 2 cut(s) 186, 228
Eam1105I GACNNNNNGTC 1 cut(s) 397
EarI CTCTTC 2 cut(s) 186, 228
EciI GGCGGA 1 cut(s) 278
Ecl136II GAGCTC 2 cut(s) 111, 161
EclXI CGGCCG 1 cut(s) 92
Eco130I CCWWGG 1 cut(s) 153
Eco24I GRGCYC 2 cut(s) 113, 163
Eco47I GGWCC 1 cut(s) 411
Eco52I CGGCCG 1 cut(s) 92
Eco53kI GAGCTC 2 cut(s) 111, 161
Eco88I CYCGRG 1 cut(s) 61
EcoICRI GAGCTC 2 cut(s) 111, 161
EcoRII CCWGG 1 cut(s) 496
EcoT14I CCWWGG 1 cut(s) 153
EcoT38I GRGCYC 2 cut(s) 113, 163
EgeI GGCGCC 2 cut(s) 6, 135
EheI GGCGCC 2 cut(s) 6, 135
ErhI CCWWGG 1 cut(s) 153
FaeI CATG 2 cut(s) 595, 631
FaiI YATR 4 cut(s) 86, 593, 629, 650
FatI CATG 2 cut(s) 591, 627
FbaI TGATCA 1 cut(s) 463
Fnu4HI GCNGC 2 cut(s) 44, 565
FokI GGATG 1 cut(s) 552
FriOI GRGCYC 2 cut(s) 113, 163
Fsp4HI GCNGC 2 cut(s) 44, 565
GlaI GCGC 3 cut(s) 6, 135, 621
GluI GCNGC 2 cut(s) 44, 565
HaeII RGCGCY 3 cut(s) 8, 137, 623
HaeIII GGCC 4 cut(s) 94, 152, 269, 320
HapII CCGG 4 cut(s) 95, 137, 179, 249
HgaI GACGC 1 cut(s) 281
HhaI GCGC 3 cut(s) 7, 136, 622
Hin1I GRCGYC 2 cut(s) 5, 134
Hin1II CATG 2 cut(s) 595, 631
Hin6I GCGC 3 cut(s) 5, 134, 620
HinP1I GCGC 3 cut(s) 5, 134, 620
HinfI GANTC 3 cut(s) 59, 76, 398
HpaII CCGG 4 cut(s) 95, 137, 179, 249
Hpy188I TCNGA 4 cut(s) 81, 102, 165, 403
Hpy188III TCNNGA 2 cut(s) 56, 587
Hpy99I CGWCG 4 cut(s) 275, 371, 395, 398
HpyAV CCTTC 7 cut(s) 142, 181, 223, 298, 368, 554, 610
HpyCH4V TGCA 2 cut(s) 432, 441
HpyF10VI GCNNNNNNNGC 4 cut(s) 95, 158, 433, 438
HpyF3I CTNAG 6 cut(s) 27, 54, 117, 162, 544, 578
Hsp92I GRCGYC 2 cut(s) 5, 134
Hsp92II CATG 2 cut(s) 595, 631
HspAI GCGC 3 cut(s) 5, 134, 620
KasI GGCGCC 2 cut(s) 4, 133
KroI GCCGGC 3 cut(s) 94, 136, 178
KroNI GCCGGC 3 cut(s) 96, 138, 180
Ksp22I TGATCA 1 cut(s) 463
Kzo9I GATC 1 cut(s) 463
LmnI GCTCC 3 cut(s) 103, 108, 158
Lsp1109I GCAGC 1 cut(s) 55
LweI GCATC 2 cut(s) 510, 530
MaeIII GTNAC 1 cut(s) 73
MalI GATC 1 cut(s) 465
MboI GATC 1 cut(s) 463
MhlI GDGCHC 2 cut(s) 113, 163
Mly113I GGCGCC 2 cut(s) 5, 134
MlyI GAGTC 3 cut(s) 53, 70, 407
MmeI TCCRAC 1 cut(s) 381
MreI CGCCGGCG 1 cut(s) 136
MroNI GCCGGC 3 cut(s) 94, 136, 178
MseI TTAA 1 cut(s) 642
MspA1I CMGCKG 1 cut(s) 46
MspI CCGG 4 cut(s) 95, 137, 179, 249
MspR9I CCNGG 1 cut(s) 498
MvaI CCWGG 1 cut(s) 498
MvnI CGCG 1 cut(s) 275
MwoI GCNNNNNNNGC 4 cut(s) 95, 158, 433, 438
NaeI GCCGGC 3 cut(s) 96, 138, 180
NarI GGCGCC 2 cut(s) 5, 134
NdeII GATC 1 cut(s) 463
NgoMIV GCCGGC 3 cut(s) 94, 136, 178
NlaIII CATG 2 cut(s) 595, 631
NlaIV GGNNCC 3 cut(s) 6, 99, 135
NmeAIII GCCGAG 4 cut(s) 33, 70, 92, 346
NmuCI GTSAC 1 cut(s) 73
PcsI WCGNNNNNNNCGW 1 cut(s) 279
PdiI GCCGGC 3 cut(s) 96, 138, 180
PkrI GCNGC 2 cut(s) 45, 566
PleI GAGTC 3 cut(s) 53, 70, 406
PluTI GGCGCC 2 cut(s) 8, 137
PpsI GAGTC 3 cut(s) 53, 70, 406
PshAI GACNNNNGTC 1 cut(s) 63
Psp124BI GAGCTC 2 cut(s) 113, 163
Psp6I CCWGG 1 cut(s) 496
PspGI CCWGG 1 cut(s) 496
PspN4I GGNNCC 3 cut(s) 6, 99, 135
PspPI GGNCC 1 cut(s) 411
PvuII CAGCTG 1 cut(s) 46
RsaI GTAC 1 cut(s) 379
RsaNI GTAC 1 cut(s) 378
SacI GAGCTC 2 cut(s) 113, 163
SaqAI TTAA 1 cut(s) 642
SatI GCNGC 2 cut(s) 44, 565
Sau3AI GATC 1 cut(s) 463
Sau96I GGNCC 1 cut(s) 411
SchI GAGTC 3 cut(s) 53, 70, 407
ScrFI CCNGG 1 cut(s) 498
SduI GDGCHC 2 cut(s) 113, 163
SfaNI GCATC 2 cut(s) 510, 530
SfoI GGCGCC 2 cut(s) 6, 135
SgrAI CRCCGGYG 1 cut(s) 136
SinI GGWCC 1 cut(s) 411
SsiI CCGC 5 cut(s) 212, 218, 263, 275, 564
SspDI GGCGCC 2 cut(s) 4, 133
SstI GAGCTC 2 cut(s) 113, 163
StyD4I CCNGG 1 cut(s) 496
StyI CCWWGG 1 cut(s) 153
TauI GCSGC 1 cut(s) 567
Tru1I TTAA 1 cut(s) 642
Tru9I TTAA 1 cut(s) 642
TseFI GTSAC 1 cut(s) 73
TseI GCWGC 1 cut(s) 43
Tsp45I GTSAC 1 cut(s) 73
TspDTI ATGAA 2 cut(s) 608, 629
TspGWI ACGGA 2 cut(s) 384, 395
VpaK11BI GGWCC 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.