MD08G1146100.v1.1

RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006028
Physical Location & Seq
Forward (+)
14540759 .. 14542217
1459 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1146100.v1.1.491

Sequence Viewer

Length: 504 bp
ATGAGCAGGCCAGGAGATTGGAACTGTAGGTCGTGCAACCATCTCAACTTCCAAAGGAGGGACTCGTGCCAGAGGTGTGGGGATCCAAAAAGTGGGGAGAGAGTTGAGTACGGAAGTTTTGGCAGTGGAAGAGGTGGAGGTGGTGGTGGTTCATATGGATTCACCACTGGTCCGGATGTCCGCCCCGGTGACTGGTACTGCACCGTTGGCAACTGTGCAGCTCACAACTTTGCCAGCCGCTCCAGTTGCTTTAAGTGTGGTGCGGCTAAGGACGAATTGTCCAGTGGTGGCGGTGGCGGCGGCTTTGAGGGGGACATGCCTAGGCCACTCAGAGGGTTTGGCTTTGGCAGCGGTGGAAGTAGCTCTGGTCGCTCCGGATGGAAATCTGGAGACTGGATTTGTACAAGACCAGGGTGCAACGAGCACAACTTCGCTAGCAGGACGGAATGTTTCAGATGCAATGCTCCAAGGGACTCCAACGATGGCACTGCTGTACTTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RanBP

Protein Analysis

168

Amino Acids

17.55

Weight (kDa)

8.45

Isoelectric Point (pI)

58.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_RanBP PF00641 128 - 157 3.1e-09 Zn-finger in Ran binding protein and others
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 92
AccBSI CCGCTC 1 cut(s) 240
AccIII TCCGGA 2 cut(s) 172, 374
AciI CCGC 7 cut(s) 181, 238, 263, 291, 297, 300, 351
AclWI GGATC 2 cut(s) 77, 90
AfaI GTAC 4 cut(s) 110, 197, 403, 495
AfiI CCNNNNNNNGG 4 cut(s) 58, 92, 192, 332
AhdI GACNNNNNGTC 1 cut(s) 277
AjnI CCWGG 2 cut(s) 10, 409
AluBI AGCT 2 cut(s) 221, 363
AluI AGCT 2 cut(s) 221, 363
Alw21I GWGCWC 1 cut(s) 426
Alw26I GTCTC 1 cut(s) 384
AlwI GGATC 2 cut(s) 77, 90
Aor13HI TCCGGA 2 cut(s) 172, 374
AoxI GGCC 2 cut(s) 8, 323
ApeKI GCWGC 2 cut(s) 218, 348
AspA2I CCTAGG 1 cut(s) 320
AspS9I GGNCC 1 cut(s) 170
AsuC2I CCSGG 1 cut(s) 186
AsuHPI GGTGA 2 cut(s) 154, 200
AsuNHI GCTAGC 1 cut(s) 434
AvaII GGWCC 1 cut(s) 170
AvrII CCTAGG 1 cut(s) 320
BamHI GGATCC 1 cut(s) 82
BauI CACGAG 1 cut(s) 64
Bbv12I GWGCWC 1 cut(s) 426
BbvI GCAGC 2 cut(s) 230, 360
BccI CCATC 3 cut(s) 48, 372, 476
BcgI CGANNNNNNTGC 2 cut(s) 470, 504
BciT130I CCWGG 2 cut(s) 12, 411
BcnI CCSGG 1 cut(s) 186
BcoDI GTCTC 1 cut(s) 384
BfaI CTAG 2 cut(s) 321, 435
BfmI CTRYAG 1 cut(s) 25
BisI GCNGC 6 cut(s) 219, 238, 264, 298, 301, 349
BlnI CCTAGG 1 cut(s) 320
BlsI GCNGC 6 cut(s) 220, 239, 265, 299, 302, 350
Bme1390I CCNGG 3 cut(s) 12, 186, 411
Bme18I GGWCC 1 cut(s) 170
BmeRI GACNNNNNGTC 1 cut(s) 277
BmgT120I GGNCC 1 cut(s) 170
BmiI GGNNCC 1 cut(s) 84
BmrFI CCNGG 3 cut(s) 12, 186, 411
BmsI GCATC 1 cut(s) 446
BmtI GCTAGC 1 cut(s) 438
BpmI CTGGAG 2 cut(s) 226, 408
Bpu10I CCTNAGC 1 cut(s) 267
BpuMI CCSGG 1 cut(s) 186
BsaBI GATNNNNATC 1 cut(s) 382
BsaJI CCNNGG 4 cut(s) 184, 320, 410, 467
BsaWI WCCGGW 2 cut(s) 172, 374
Bsc4I CCNNNNNNNGG 4 cut(s) 58, 92, 192, 332
Bse1I ACTGG 5 cut(s) 172, 197, 243, 282, 398
Bse3DI GCAATG 1 cut(s) 466
Bse8I GATNNNNATC 1 cut(s) 382
BseAI TCCGGA 2 cut(s) 172, 374
BseBI CCWGG 2 cut(s) 12, 411
BseDI CCNNGG 4 cut(s) 184, 320, 410, 467
BseGI GGATG 2 cut(s) 181, 383
BseJI GATNNNNATC 1 cut(s) 382
BseLI CCNNNNNNNGG 4 cut(s) 58, 92, 192, 332
BseMI GCAATG 1 cut(s) 466
BseMII CTCAG 1 cut(s) 343
BseNI ACTGG 5 cut(s) 172, 197, 243, 282, 398
BseXI GCAGC 2 cut(s) 230, 360
BsgI GTGCAG 2 cut(s) 184, 237
BshFI GGCC 2 cut(s) 10, 325
BsiHKAI GWGCWC 1 cut(s) 426
BsiSI CCGG 3 cut(s) 173, 186, 375
BslFI GGGAC 3 cut(s) 74, 326, 485
BslI CCNNNNNNNGG 4 cut(s) 58, 92, 192, 332
BsmAI GTCTC 1 cut(s) 384
BsmFI GGGAC 3 cut(s) 74, 326, 485
BsnI GGCC 2 cut(s) 10, 325
Bsp1286I GDGCHC 1 cut(s) 426
Bsp13I TCCGGA 2 cut(s) 172, 374
Bsp1407I TGTACA 1 cut(s) 401
Bsp143I GATC 1 cut(s) 82
BspACI CCGC 7 cut(s) 181, 238, 263, 291, 297, 300, 351
BspANI GGCC 2 cut(s) 10, 325
BspCNI CTCAG 1 cut(s) 342
BspEI TCCGGA 2 cut(s) 172, 374
BspLI GGNNCC 1 cut(s) 84
BspOI GCTAGC 1 cut(s) 438
BspPI GGATC 2 cut(s) 77, 90
BsrBI CCGCTC 1 cut(s) 240
BsrDI GCAATG 1 cut(s) 466
BsrGI TGTACA 1 cut(s) 401
BsrI ACTGG 5 cut(s) 172, 197, 243, 282, 398
BssECI CCNNGG 4 cut(s) 184, 320, 410, 467
BssMI GATC 1 cut(s) 82
BssSI CACGAG 1 cut(s) 64
BssT1I CCWWGG 2 cut(s) 320, 467
Bst2BI CACGAG 1 cut(s) 64
Bst2UI CCWGG 2 cut(s) 12, 411
Bst4CI ACNGT 3 cut(s) 26, 205, 215
Bst6I CTCTTC 1 cut(s) 124
BstAUI TGTACA 1 cut(s) 401
BstC8I GCNNGC 3 cut(s) 8, 235, 436
BstDEI CTNAG 2 cut(s) 267, 329
BstF5I GGATG 2 cut(s) 181, 383
BstKTI GATC 1 cut(s) 85
BstMAI GTCTC 1 cut(s) 384
BstMBI GATC 1 cut(s) 82
BstMWI GCNNNNNNNGC 5 cut(s) 207, 246, 297, 348, 369
BstNI CCWGG 2 cut(s) 12, 411
BstNSI RCATGY 1 cut(s) 319
BstSCI CCNGG 3 cut(s) 10, 184, 409
BstSFI CTRYAG 1 cut(s) 25
BstV1I GCAGC 2 cut(s) 230, 360
BstX2I RGATCY 1 cut(s) 82
BstXI CCANNNNNNTGG 2 cut(s) 18, 77
BstYI RGATCY 1 cut(s) 82
BsuRI GGCC 2 cut(s) 10, 325
BtsCI GGATG 2 cut(s) 181, 383
BtsI GCAGTG 2 cut(s) 130, 486
BtsIMutI CAGTG 4 cut(s) 130, 165, 289, 486
Cac8I GCNNGC 3 cut(s) 8, 235, 436
Cfr13I GGNCC 1 cut(s) 170
Csp6I GTAC 4 cut(s) 109, 196, 402, 494
CviAII CATG 1 cut(s) 316
CviJI RGCY 8 cut(s) 10, 221, 237, 266, 303, 325, 342, 363
CviKI_1 RGCY 8 cut(s) 10, 221, 237, 266, 303, 325, 342, 363
CviQI GTAC 4 cut(s) 109, 196, 402, 494
DdeI CTNAG 2 cut(s) 267, 329
DpnI GATC 1 cut(s) 84
DpnII GATC 1 cut(s) 82
DriI GACNNNNNGTC 1 cut(s) 277
Eam1104I CTCTTC 1 cut(s) 124
Eam1105I GACNNNNNGTC 1 cut(s) 277
EarI CTCTTC 1 cut(s) 124
EciI GGCGGA 1 cut(s) 170
Eco130I CCWWGG 2 cut(s) 320, 467
Eco47I GGWCC 1 cut(s) 170
EcoRII CCWGG 2 cut(s) 10, 409
EcoT14I CCWWGG 2 cut(s) 320, 467
ErhI CCWWGG 2 cut(s) 320, 467
FaeI CATG 1 cut(s) 319
FaiI YATR 3 cut(s) 154, 156, 317
FaqI GGGAC 3 cut(s) 74, 326, 485
FatI CATG 1 cut(s) 315
FauNDI CATATG 1 cut(s) 154
Fnu4HI GCNGC 6 cut(s) 219, 238, 264, 298, 301, 349
FokI GGATG 2 cut(s) 188, 390
Fsp4HI GCNGC 6 cut(s) 219, 238, 264, 298, 301, 349
FspBI CTAG 2 cut(s) 321, 435
GluI GCNGC 6 cut(s) 219, 238, 264, 298, 301, 349
GsuI CTGGAG 2 cut(s) 226, 408
HaeIII GGCC 2 cut(s) 10, 325
HapII CCGG 3 cut(s) 173, 186, 375
Hin1II CATG 1 cut(s) 319
HinfI GANTC 3 cut(s) 62, 159, 473
HpaII CCGG 3 cut(s) 173, 186, 375
HphI GGTGA 2 cut(s) 154, 200
Hpy188I TCNGA 2 cut(s) 332, 455
Hpy188III TCNNGA 3 cut(s) 173, 375, 387
HpyCH4III ACNGT 3 cut(s) 26, 205, 215
HpyCH4V TGCA 5 cut(s) 36, 201, 218, 417, 459
HpyF10VI GCNNNNNNNGC 5 cut(s) 207, 246, 297, 348, 369
HpyF3I CTNAG 2 cut(s) 267, 329
Hsp92II CATG 1 cut(s) 319
Kpn2I TCCGGA 2 cut(s) 172, 374
Kzo9I GATC 1 cut(s) 82
LmnI GCTCC 3 cut(s) 245, 377, 469
Lsp1109I GCAGC 2 cut(s) 230, 360
LweI GCATC 1 cut(s) 446
MaeI CTAG 2 cut(s) 321, 435
MaeIII GTNAC 1 cut(s) 188
MalI GATC 1 cut(s) 84
MbiI CCGCTC 1 cut(s) 240
MboI GATC 1 cut(s) 82
MboII GAAGA 1 cut(s) 141
MflI RGATCY 1 cut(s) 82
MhlI GDGCHC 1 cut(s) 426
MluCI AATT 1 cut(s) 275
MlyI GAGTC 2 cut(s) 56, 467
MmeI TCCRAC 1 cut(s) 501
MnlI CCTC 6 cut(s) 51, 66, 125, 131, 301, 326
MroI TCCGGA 2 cut(s) 172, 374
MseI TTAA 1 cut(s) 252
MspA1I CMGCKG 1 cut(s) 351
MspI CCGG 3 cut(s) 173, 186, 375
MspR9I CCNGG 3 cut(s) 12, 186, 411
MvaI CCWGG 2 cut(s) 12, 411
MwoI GCNNNNNNNGC 5 cut(s) 207, 246, 297, 348, 369
NciI CCSGG 1 cut(s) 186
NdeI CATATG 1 cut(s) 154
NdeII GATC 1 cut(s) 82
NheI GCTAGC 1 cut(s) 434
NlaIII CATG 1 cut(s) 319
NlaIV GGNNCC 1 cut(s) 84
NmuCI GTSAC 1 cut(s) 188
NspI RCATGY 1 cut(s) 319
PfeI GAWTC 1 cut(s) 159
PflMI CCANNNNNTGG 1 cut(s) 92
PkrI GCNGC 6 cut(s) 220, 239, 265, 299, 302, 350
PleI GAGTC 2 cut(s) 56, 467
PpsI GAGTC 2 cut(s) 56, 467
Psp6I CCWGG 2 cut(s) 10, 409
PspGI CCWGG 2 cut(s) 10, 409
PspN4I GGNNCC 1 cut(s) 84
PspPI GGNCC 1 cut(s) 170
PsuI RGATCY 1 cut(s) 82
RsaI GTAC 4 cut(s) 110, 197, 403, 495
RsaNI GTAC 4 cut(s) 109, 196, 402, 494
SaqAI TTAA 1 cut(s) 252
SatI GCNGC 6 cut(s) 219, 238, 264, 298, 301, 349
Sau3AI GATC 1 cut(s) 82
Sau96I GGNCC 1 cut(s) 170
SchI GAGTC 2 cut(s) 56, 467
ScrFI CCNGG 3 cut(s) 12, 186, 411
SduI GDGCHC 1 cut(s) 426
SetI ASST 6 cut(s) 32, 77, 136, 142, 223, 365
SfaNI GCATC 1 cut(s) 446
SfcI CTRYAG 1 cut(s) 25
SinI GGWCC 1 cut(s) 170
Sse9I AATT 1 cut(s) 275
SsiI CCGC 7 cut(s) 181, 238, 263, 291, 297, 300, 351
SspMI CTAG 2 cut(s) 321, 435
StyD4I CCNGG 3 cut(s) 10, 184, 409
StyI CCWWGG 2 cut(s) 320, 467
TaaI ACNGT 3 cut(s) 26, 205, 215
TasI AATT 1 cut(s) 275
TatI WGTACW 2 cut(s) 401, 493
TauI GCSGC 4 cut(s) 240, 266, 300, 303
TfiI GAWTC 1 cut(s) 159
Tru1I TTAA 1 cut(s) 252
Tru9I TTAA 1 cut(s) 252
TscAI CASTG 4 cut(s) 130, 172, 289, 493
TseFI GTSAC 1 cut(s) 188
TseI GCWGC 2 cut(s) 218, 348
Tsp45I GTSAC 1 cut(s) 188
TspDTI ATGAA 1 cut(s) 141
TspGWI ACGGA 2 cut(s) 126, 458
TspRI CASTG 4 cut(s) 130, 172, 289, 493
Van91I CCANNNNNTGG 1 cut(s) 92
VpaK11BI GGWCC 1 cut(s) 170
XceI RCATGY 1 cut(s) 319
XmaJI CCTAGG 1 cut(s) 320
XspI CTAG 2 cut(s) 321, 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.