pycom08g12320

RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0006028
Physical Location & Seq
Forward (+)
10643370 .. 10644002
633 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g12320.1

Sequence Viewer

Length: 480 bp
ATGAGCAGGCCAGGAGATTGGAACTGTAGGTCGTGCAACCATCTCAACTTCCAAAGGAGGGACTCGTGCCAGAGGTGTGGGGATCCAAAAAGTGGGGAGAGAGTTGAGTACGGAAGAGGTGGTGGTGGTTCATATGGATTCACCACTGGTCCGGATGTCCGCCCCGGTGACTGGTACTGCACCGTTGGCAACTGTGCAGCTCACAACTTTGCCAGCCGCTCCAGTTGCTTCAAGTGTGGTGCGTCTAAGGACGAATTGTCCACTGGTGGCGGTGGCGGCGGCTTTGAGGGGGACATGCCTAGGCCACTCAGAGGGTTTGGTTTTGGCAGCGGTGGAAGTAGCTCTGGTCGCTCCGGATGGAAATCTGGAGACTGGATTTGTACAAGACCTGGGTGCAATGAGCACAATTTCGCTAGCAGGACGGAATGTTTCAGATGCAATGCTCCAAGGGACTCCAACGATGGCAAGTTTCCATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RanBP

Protein Analysis

160

Amino Acids

17.07

Weight (kDa)

8.65

Isoelectric Point (pI)

59.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_RanBP PF00641 121 - 150 2.9e-09 Zn-finger in Ran binding protein and others
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 92
AccBSI CCGCTC 1 cut(s) 219
AccIII TCCGGA 2 cut(s) 151, 353
AciI CCGC 6 cut(s) 160, 217, 270, 276, 279, 330
AclWI GGATC 2 cut(s) 77, 90
AfaI GTAC 3 cut(s) 110, 176, 382
AfiI CCNNNNNNNGG 4 cut(s) 58, 92, 171, 311
AgsI TTSAA 1 cut(s) 232
AhdI GACNNNNNGTC 1 cut(s) 256
AjnI CCWGG 2 cut(s) 10, 388
AluBI AGCT 2 cut(s) 200, 342
AluI AGCT 2 cut(s) 200, 342
Alw21I GWGCWC 1 cut(s) 405
Alw26I GTCTC 1 cut(s) 363
AlwI GGATC 2 cut(s) 77, 90
Aor13HI TCCGGA 2 cut(s) 151, 353
AoxI GGCC 2 cut(s) 8, 302
ApeKI GCWGC 2 cut(s) 197, 327
AspA2I CCTAGG 1 cut(s) 299
AspS9I GGNCC 1 cut(s) 149
AsuC2I CCSGG 1 cut(s) 165
AsuHPI GGTGA 2 cut(s) 133, 179
AsuNHI GCTAGC 1 cut(s) 413
AvaII GGWCC 1 cut(s) 149
AvrII CCTAGG 1 cut(s) 299
BamHI GGATCC 1 cut(s) 82
BauI CACGAG 1 cut(s) 64
Bbv12I GWGCWC 1 cut(s) 405
BbvI GCAGC 2 cut(s) 209, 339
BccI CCATC 3 cut(s) 48, 351, 455
BciT130I CCWGG 2 cut(s) 12, 390
BcnI CCSGG 1 cut(s) 165
BcoDI GTCTC 1 cut(s) 363
BfaI CTAG 2 cut(s) 300, 414
BfmI CTRYAG 1 cut(s) 25
BisI GCNGC 5 cut(s) 198, 217, 277, 280, 328
BlnI CCTAGG 1 cut(s) 299
BlsI GCNGC 5 cut(s) 199, 218, 278, 281, 329
Bme1390I CCNGG 3 cut(s) 12, 165, 390
Bme18I GGWCC 1 cut(s) 149
BmeRI GACNNNNNGTC 1 cut(s) 256
BmgT120I GGNCC 1 cut(s) 149
BmiI GGNNCC 1 cut(s) 84
BmrFI CCNGG 3 cut(s) 12, 165, 390
BmsI GCATC 1 cut(s) 425
BmtI GCTAGC 1 cut(s) 417
BpmI CTGGAG 2 cut(s) 205, 387
BpuMI CCSGG 1 cut(s) 165
BsaBI GATNNNNATC 1 cut(s) 361
BsaJI CCNNGG 4 cut(s) 163, 299, 389, 446
BsaWI WCCGGW 2 cut(s) 151, 353
Bsc4I CCNNNNNNNGG 4 cut(s) 58, 92, 171, 311
Bse1I ACTGG 5 cut(s) 151, 176, 222, 268, 377
Bse3DI GCAATG 2 cut(s) 403, 445
Bse8I GATNNNNATC 1 cut(s) 361
BseAI TCCGGA 2 cut(s) 151, 353
BseBI CCWGG 2 cut(s) 12, 390
BseDI CCNNGG 4 cut(s) 163, 299, 389, 446
BseGI GGATG 2 cut(s) 160, 362
BseJI GATNNNNATC 1 cut(s) 361
BseLI CCNNNNNNNGG 4 cut(s) 58, 92, 171, 311
BseMI GCAATG 2 cut(s) 403, 445
BseMII CTCAG 1 cut(s) 322
BseNI ACTGG 5 cut(s) 151, 176, 222, 268, 377
BseXI GCAGC 2 cut(s) 209, 339
BsgI GTGCAG 2 cut(s) 163, 216
BshFI GGCC 2 cut(s) 10, 304
BsiHKAI GWGCWC 1 cut(s) 405
BsiSI CCGG 3 cut(s) 152, 165, 354
BslFI GGGAC 3 cut(s) 74, 305, 464
BslI CCNNNNNNNGG 4 cut(s) 58, 92, 171, 311
BsmAI GTCTC 1 cut(s) 363
BsmFI GGGAC 3 cut(s) 74, 305, 464
BsnI GGCC 2 cut(s) 10, 304
Bsp1286I GDGCHC 1 cut(s) 405
Bsp13I TCCGGA 2 cut(s) 151, 353
Bsp1407I TGTACA 1 cut(s) 380
Bsp143I GATC 1 cut(s) 82
BspACI CCGC 6 cut(s) 160, 217, 270, 276, 279, 330
BspANI GGCC 2 cut(s) 10, 304
BspCNI CTCAG 1 cut(s) 321
BspEI TCCGGA 2 cut(s) 151, 353
BspLI GGNNCC 1 cut(s) 84
BspOI GCTAGC 1 cut(s) 417
BspPI GGATC 2 cut(s) 77, 90
BsrBI CCGCTC 1 cut(s) 219
BsrDI GCAATG 2 cut(s) 403, 445
BsrGI TGTACA 1 cut(s) 380
BsrI ACTGG 5 cut(s) 151, 176, 222, 268, 377
BssECI CCNNGG 4 cut(s) 163, 299, 389, 446
BssMI GATC 1 cut(s) 82
BssSI CACGAG 1 cut(s) 64
BssT1I CCWWGG 2 cut(s) 299, 446
Bst2BI CACGAG 1 cut(s) 64
Bst2UI CCWGG 2 cut(s) 12, 390
Bst4CI ACNGT 3 cut(s) 26, 184, 194
Bst6I CTCTTC 1 cut(s) 109
BstAUI TGTACA 1 cut(s) 380
BstC8I GCNNGC 3 cut(s) 8, 214, 415
BstDEI CTNAG 2 cut(s) 246, 308
BstF5I GGATG 2 cut(s) 160, 362
BstKTI GATC 1 cut(s) 85
BstMAI GTCTC 1 cut(s) 363
BstMBI GATC 1 cut(s) 82
BstMWI GCNNNNNNNGC 4 cut(s) 186, 225, 276, 348
BstNI CCWGG 2 cut(s) 12, 390
BstNSI RCATGY 1 cut(s) 298
BstSCI CCNGG 3 cut(s) 10, 163, 388
BstSFI CTRYAG 1 cut(s) 25
BstV1I GCAGC 2 cut(s) 209, 339
BstX2I RGATCY 1 cut(s) 82
BstXI CCANNNNNNTGG 2 cut(s) 18, 77
BstYI RGATCY 1 cut(s) 82
BsuRI GGCC 2 cut(s) 10, 304
BtsCI GGATG 2 cut(s) 160, 362
BtsIMutI CAGTG 2 cut(s) 144, 261
Cac8I GCNNGC 3 cut(s) 8, 214, 415
Cfr13I GGNCC 1 cut(s) 149
CseI GACGC 1 cut(s) 231
Csp6I GTAC 3 cut(s) 109, 175, 381
CviAII CATG 1 cut(s) 295
CviJI RGCY 6 cut(s) 10, 200, 216, 282, 304, 342
CviKI_1 RGCY 6 cut(s) 10, 200, 216, 282, 304, 342
CviQI GTAC 3 cut(s) 109, 175, 381
DdeI CTNAG 2 cut(s) 246, 308
DpnI GATC 1 cut(s) 84
DpnII GATC 1 cut(s) 82
DriI GACNNNNNGTC 1 cut(s) 256
Eam1104I CTCTTC 1 cut(s) 109
Eam1105I GACNNNNNGTC 1 cut(s) 256
EarI CTCTTC 1 cut(s) 109
EciI GGCGGA 1 cut(s) 149
Eco130I CCWWGG 2 cut(s) 299, 446
Eco47I GGWCC 1 cut(s) 149
EcoRII CCWGG 2 cut(s) 10, 388
EcoT14I CCWWGG 2 cut(s) 299, 446
ErhI CCWWGG 2 cut(s) 299, 446
FaeI CATG 1 cut(s) 298
FaiI YATR 3 cut(s) 133, 135, 296
FaqI GGGAC 3 cut(s) 74, 305, 464
FatI CATG 1 cut(s) 294
FauNDI CATATG 1 cut(s) 133
Fnu4HI GCNGC 5 cut(s) 198, 217, 277, 280, 328
FokI GGATG 2 cut(s) 167, 369
Fsp4HI GCNGC 5 cut(s) 198, 217, 277, 280, 328
FspBI CTAG 2 cut(s) 300, 414
GluI GCNGC 5 cut(s) 198, 217, 277, 280, 328
GsuI CTGGAG 2 cut(s) 205, 387
HaeIII GGCC 2 cut(s) 10, 304
HapII CCGG 3 cut(s) 152, 165, 354
HgaI GACGC 1 cut(s) 231
Hin1II CATG 1 cut(s) 298
HinfI GANTC 3 cut(s) 62, 138, 452
HpaII CCGG 3 cut(s) 152, 165, 354
HphI GGTGA 2 cut(s) 133, 179
Hpy166II GTNNAC 1 cut(s) 261
Hpy188I TCNGA 2 cut(s) 311, 434
Hpy188III TCNNGA 3 cut(s) 152, 354, 366
Hpy8I GTNNAC 1 cut(s) 261
HpyCH4III ACNGT 3 cut(s) 26, 184, 194
HpyCH4V TGCA 5 cut(s) 36, 180, 197, 396, 438
HpyF10VI GCNNNNNNNGC 4 cut(s) 186, 225, 276, 348
HpyF3I CTNAG 2 cut(s) 246, 308
Hsp92II CATG 1 cut(s) 298
Kpn2I TCCGGA 2 cut(s) 151, 353
Kzo9I GATC 1 cut(s) 82
LmnI GCTCC 3 cut(s) 224, 356, 448
Lsp1109I GCAGC 2 cut(s) 209, 339
LweI GCATC 1 cut(s) 425
MaeI CTAG 2 cut(s) 300, 414
MaeIII GTNAC 1 cut(s) 167
MalI GATC 1 cut(s) 84
MbiI CCGCTC 1 cut(s) 219
MboI GATC 1 cut(s) 82
MboII GAAGA 1 cut(s) 126
MflI RGATCY 1 cut(s) 82
MhlI GDGCHC 1 cut(s) 405
MluCI AATT 2 cut(s) 254, 406
MlyI GAGTC 2 cut(s) 56, 446
MmeI TCCRAC 1 cut(s) 480
MnlI CCTC 5 cut(s) 51, 66, 110, 280, 305
MroI TCCGGA 2 cut(s) 151, 353
MseI TTAA 1 cut(s) 478
MspA1I CMGCKG 1 cut(s) 330
MspI CCGG 3 cut(s) 152, 165, 354
MspR9I CCNGG 3 cut(s) 12, 165, 390
MvaI CCWGG 2 cut(s) 12, 390
MwoI GCNNNNNNNGC 4 cut(s) 186, 225, 276, 348
NciI CCSGG 1 cut(s) 165
NdeI CATATG 1 cut(s) 133
NdeII GATC 1 cut(s) 82
NheI GCTAGC 1 cut(s) 413
NlaIII CATG 1 cut(s) 298
NlaIV GGNNCC 1 cut(s) 84
NmuCI GTSAC 1 cut(s) 167
NspI RCATGY 1 cut(s) 298
PfeI GAWTC 1 cut(s) 138
PflMI CCANNNNNTGG 1 cut(s) 92
PkrI GCNGC 5 cut(s) 199, 218, 278, 281, 329
PleI GAGTC 2 cut(s) 56, 446
PpsI GAGTC 2 cut(s) 56, 446
Psp6I CCWGG 2 cut(s) 10, 388
PspGI CCWGG 2 cut(s) 10, 388
PspN4I GGNNCC 1 cut(s) 84
PspPI GGNCC 1 cut(s) 149
PsuI RGATCY 1 cut(s) 82
RsaI GTAC 3 cut(s) 110, 176, 382
RsaNI GTAC 3 cut(s) 109, 175, 381
SaqAI TTAA 1 cut(s) 478
SatI GCNGC 5 cut(s) 198, 217, 277, 280, 328
Sau3AI GATC 1 cut(s) 82
Sau96I GGNCC 1 cut(s) 149
SchI GAGTC 2 cut(s) 56, 446
ScrFI CCNGG 3 cut(s) 12, 165, 390
SduI GDGCHC 1 cut(s) 405
SetI ASST 6 cut(s) 32, 77, 121, 202, 344, 391
SfaNI GCATC 1 cut(s) 425
SfcI CTRYAG 1 cut(s) 25
SinI GGWCC 1 cut(s) 149
Sse9I AATT 2 cut(s) 254, 406
SsiI CCGC 6 cut(s) 160, 217, 270, 276, 279, 330
SspMI CTAG 2 cut(s) 300, 414
StyD4I CCNGG 3 cut(s) 10, 163, 388
StyI CCWWGG 2 cut(s) 299, 446
TaaI ACNGT 3 cut(s) 26, 184, 194
TasI AATT 2 cut(s) 254, 406
TatI WGTACW 1 cut(s) 380
TauI GCSGC 3 cut(s) 219, 279, 282
TfiI GAWTC 1 cut(s) 138
Tru1I TTAA 1 cut(s) 478
Tru9I TTAA 1 cut(s) 478
TscAI CASTG 2 cut(s) 151, 268
TseFI GTSAC 1 cut(s) 167
TseI GCWGC 2 cut(s) 197, 327
Tsp45I GTSAC 1 cut(s) 167
TspDTI ATGAA 1 cut(s) 120
TspGWI ACGGA 2 cut(s) 126, 437
TspRI CASTG 2 cut(s) 151, 268
Van91I CCANNNNNTGG 1 cut(s) 92
VpaK11BI GGWCC 1 cut(s) 149
XceI RCATGY 1 cut(s) 298
XmaJI CCTAGG 1 cut(s) 299
XspI CTAG 2 cut(s) 300, 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.