MD08G1176400.v1.1

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006242
Physical Location & Seq
Reverse (-)
21395642 .. 21398438
2797 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1176400.v1.1.491

Sequence Viewer

Length: 1434 bp
ATGGGGGCTCTTTTGAGTTTGGCTGGTCCCAAGTCTGAGAGAAATGAGTACCATGAGATCTCTCAGAATGACACCTGCAAGAGGCAAAAAACGTCACCATGCACTTTTGAGGAGAGCCAAAGACTAATTCCCACTCTTCCTGATGAGCTATCGATCCAAATTATTGCTAGACTCCCTAGAATTTGCTACTTCAATATCAGGCTGGTTTCCCGGAAGTGGAAGGAAACTGTTACTAGCTCTGAACTATTTAAATTGAGAAAAGAACTTGGTAGAAGTGAAGAATGGCTCTACTTGTTGACGAAAATTGAAGAGGATAAACTTTCCTGGCATGCTTTAGATCCCCTGTCAAGAAGGTGGCAGAGGCTACCTCAGATGCCCAATGTAGTTTATGAAGAAGAAATCAGCAAGAGTTCTTCTAGGTTTTCGATGTGGAATATGGTGGGCCCAAGCATCAAAATTGCTCATACTATTAGAGGCTGGTTAGGGAGAAAGAACGCATTTGAACAAATGCCGTTTTGTGGTTGTGCCATTGGTGTTGTTGATGGATGCCTTTATGTTCTAGGTGGATTTTATAAAGCTACGACCGTGAGGTGCGTCTGGAGATTTGATCCAATTCAGAATGCATGGAGTGAAGTGACAGCCATGTCTGCAAGTAGAGCCTATTGTAAGACAGGTATTCTGAATAACAAACTTTATGTTGTTGGAGGGGTTACTCGGGAACGAGGCGGATTGATCCCTCTTCAGTCTGCTGAAGTTTTTGAGCCCTCCACCGGTACATGGTCTGAAGTACCAAGCATGCCATTCTCAAGGGCTCGAGCGCTACCCACTGCCTTTTTGACTGACATGCTAAGGCCCATTGCCACCGGGTTGACTCCCTACATGGGAAGGCTATGTGTATCCCAGAGTCTGTATTCATGGCCCTTTTTTGTTGATGTCGGGGGAGAAATCTATGATCCCGAAACAAATTCTTGGGATGAAATGCCTCTTGGCATGGGAGATGGTTGGCCTGCTCGGCAGGCTGGTACAAAGTTGAGCGTTGTGGTAGATGGTGAATTGTATGCTTTTGATCCTTCGAGTTCTTTGGATAGTGGTAAGATCAAGGTGTACGATCAAGGAGAAGATGCTTGGAAAGTTGTTATAGGAAAAGTCCCTATTCATGACTTTGCTGGTTCAGATTCTCCATATTTACTTGCTGGTTTTCATGGAAAACTTCATGTCATTACAAAAGATGCGAATCATGAAATCGCAGTTCTGCGTGCTGACCTGTGCTGTAATTTGGGTTCTTTGTCACCAACCTTAGCTCCTCTCCTGGCTAGCTCCTCAGATGAACACTCCGACGCACCGGCAGAATCAGACGCAGTTGTGTGGAAGATCATTGGGACTCGGGATTTTGGTTCAGGTGAATTGGTTGGCTGTCAAGTTCTTGATCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_1

Protein Analysis

478

Amino Acids

53.08

Weight (kDa)

5.86

Isoelectric Point (pI)

49.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 45 - 84 5.3e-09 F-box domain
Kelch_FKB95 PF25210 169 - 412 2.5e-17 FKB95, Kelch-repeats domain
Kelch_1 PF01344 171 - 216 2.1e-12 Kelch motif
Kelch_KLHDC2_KLHL20_DRC7 PF24681 175 - 266 5.7e-08 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_Calicin PF13964 177 - 269 2.2e-07 Calicin, beta-propeller domain
Kelch_1 PF01344 219 - 267 4.2e-11 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 573
AarI CACCTGC 1 cut(s) 83
AasI GACNNNNNNGTC 1 cut(s) 643
Acc36I ACCTGC 1 cut(s) 83
AciI CCGC 1 cut(s) 726
AclWI GGATC 6 cut(s) 148, 332, 602, 727, 947, 1061
AcsI RAATTY 2 cut(s) 180, 964
AcuI CTGAAG 3 cut(s) 725, 771, 804
AfaI GTAC 5 cut(s) 50, 775, 789, 1024, 1106
AfeI AGCGCT 1 cut(s) 819
AfiI CCNNNNNNNGG 6 cut(s) 81, 216, 518, 770, 777, 881
AgeI ACCGGT 1 cut(s) 770
AgsI TTSAA 3 cut(s) 193, 308, 503
AjnI CCWGG 2 cut(s) 323, 1308
AjuI GAANNNNNNNTTGG 4 cut(s) 111, 143, 969, 1001
AluBI AGCT 5 cut(s) 148, 237, 578, 1301, 1317
AluI AGCT 5 cut(s) 148, 237, 578, 1301, 1317
AlwI GGATC 6 cut(s) 148, 332, 602, 727, 947, 1061
AlwNI CAGNNNCTG 1 cut(s) 907
Ama87I CYCGRG 3 cut(s) 714, 813, 1383
Aor51HI AGCGCT 1 cut(s) 819
AoxI GGCC 4 cut(s) 442, 851, 917, 1004
ApaI GGGCCC 1 cut(s) 446
ApoI RAATTY 2 cut(s) 180, 964
AsiGI ACCGGT 1 cut(s) 770
AspLEI GCGC 1 cut(s) 820
AspS9I GGNCC 5 cut(s) 26, 442, 443, 852, 918
AsuC2I CCSGG 2 cut(s) 211, 865
AsuHPI GGTGA 4 cut(s) 87, 1061, 1281, 1412
AsuNHI GCTAGC 1 cut(s) 1313
AvaI CYCGRG 3 cut(s) 714, 813, 1383
AvaII GGWCC 1 cut(s) 26
BaeGI GKGCMC 1 cut(s) 446
BanII GRGCYC 4 cut(s) 10, 446, 765, 814
BccI CCATC 3 cut(s) 536, 992, 1040
BceAI ACGGC 1 cut(s) 496
BciT130I CCWGG 2 cut(s) 325, 1310
BciVI GTATCC 1 cut(s) 907
BcnI CCSGG 2 cut(s) 211, 865
BfaI CTAG 6 cut(s) 168, 177, 234, 417, 560, 1314
BfoI RGCGCY 1 cut(s) 821
BfuAI ACCTGC 1 cut(s) 83
BfuI GTATCC 1 cut(s) 907
BglI GCCNNNNNGGC 1 cut(s) 1012
BglII AGATCT 1 cut(s) 57
Bme1390I CCNGG 4 cut(s) 211, 325, 865, 1310
Bme18I GGWCC 1 cut(s) 26
BmeT110I CYCGRG 3 cut(s) 714, 813, 1383
BmgT120I GGNCC 5 cut(s) 26, 442, 443, 852, 918
BmiI GGNNCC 2 cut(s) 28, 444
BmrFI CCNGG 4 cut(s) 211, 325, 865, 1310
BmsI GCATC 5 cut(s) 363, 459, 536, 1111, 1219
BmtI GCTAGC 1 cut(s) 1317
BplI GAGNNNNNCTC 2 cut(s) 352, 384
BpmI CTGGAG 1 cut(s) 619
Bpu10I CCTNAGC 2 cut(s) 848, 1297
BpuEI CTTGAG 1 cut(s) 790
BpuMI CCSGG 2 cut(s) 211, 865
Bsa29I ATCGAT 1 cut(s) 152
BsaBI GATNNNNATC 1 cut(s) 1233
BsaWI WCCGGW 1 cut(s) 770
BsaXI ACNNNNNCTCC 2 cut(s) 1285, 1315
Bsc4I CCNNNNNNNGG 6 cut(s) 81, 216, 518, 770, 777, 881
Bse118I RCCGGY 2 cut(s) 770, 1342
Bse3DI GCAATG 1 cut(s) 855
Bse8I GATNNNNATC 1 cut(s) 1233
BseBI CCWGG 2 cut(s) 325, 1310
BseCI ATCGAT 1 cut(s) 152
BseGI GGATG 2 cut(s) 551, 979
BseJI GATNNNNATC 1 cut(s) 1233
BseLI CCNNNNNNNGG 6 cut(s) 81, 216, 518, 770, 777, 881
BseMI GCAATG 1 cut(s) 855
BseMII CTCAG 4 cut(s) 27, 77, 383, 1335
BseRI GAGGAG 3 cut(s) 125, 1293, 1309
BseSI GKGCMC 1 cut(s) 446
Bsh1285I CGRYCG 1 cut(s) 585
BshFI GGCC 4 cut(s) 444, 853, 919, 1006
BshTI ACCGGT 1 cut(s) 770
BshVI ATCGAT 1 cut(s) 152
BsiEI CGRYCG 1 cut(s) 585
BsiHKCI CYCGRG 3 cut(s) 714, 813, 1383
BsiSI CCGG 4 cut(s) 211, 771, 864, 1343
BslFI GGGAC 3 cut(s) 12, 1133, 1393
BslI CCNNNNNNNGG 6 cut(s) 81, 216, 518, 770, 777, 881
BsmFI GGGAC 3 cut(s) 12, 1133, 1393
BsmI GAATGC 1 cut(s) 625
BsnI GGCC 4 cut(s) 444, 853, 919, 1006
BsoBI CYCGRG 3 cut(s) 714, 813, 1383
Bsp120I GGGCCC 1 cut(s) 442
Bsp1286I GDGCHC 4 cut(s) 10, 446, 765, 814
BspACI CCGC 1 cut(s) 726
BspANI GGCC 4 cut(s) 444, 853, 919, 1006
BspCNI CTCAG 4 cut(s) 28, 76, 382, 1334
BspDI ATCGAT 1 cut(s) 152
BspHI TCATGA 2 cut(s) 1156, 1237
BspLI GGNNCC 2 cut(s) 28, 444
BspMI ACCTGC 1 cut(s) 83
BspOI GCTAGC 1 cut(s) 1317
BspPI GGATC 6 cut(s) 148, 332, 602, 727, 947, 1061
BsrDI GCAATG 1 cut(s) 855
BsrFI RCCGGY 2 cut(s) 770, 1342
BssAI RCCGGY 2 cut(s) 770, 1342
Bst2UI CCWGG 2 cut(s) 325, 1310
Bst4CI ACNGT 2 cut(s) 229, 586
Bst6I CTCTTC 3 cut(s) 141, 303, 744
BstC8I GCNNGC 6 cut(s) 330, 797, 1008, 1017, 1257, 1315
BstDEI CTNAG 6 cut(s) 36, 63, 369, 848, 1297, 1321
BstENI CCTNNNNNAGG 1 cut(s) 79
BstF5I GGATG 2 cut(s) 551, 979
BstH2I RGCGCY 1 cut(s) 821
BstHHI GCGC 1 cut(s) 820
BstMCI CGRYCG 1 cut(s) 585
BstMWI GCNNNNNNNGC 4 cut(s) 647, 656, 1012, 1016
BstNI CCWGG 2 cut(s) 325, 1310
BstNSI RCATGY 3 cut(s) 332, 799, 847
BstSCI CCNGG 4 cut(s) 209, 323, 863, 1308
BstSLI GKGCMC 1 cut(s) 446
BstX2I RGATCY 2 cut(s) 57, 337
BstYI RGATCY 2 cut(s) 57, 337
Bsu15I ATCGAT 1 cut(s) 152
BsuI GTATCC 1 cut(s) 907
BsuRI GGCC 4 cut(s) 444, 853, 919, 1006
BsuTUI ATCGAT 1 cut(s) 152
BtsCI GGATG 2 cut(s) 551, 979
BtsI GCAGTG 1 cut(s) 825
BtsIMutI CAGTG 1 cut(s) 825
BveI ACCTGC 1 cut(s) 83
Cac8I GCNNGC 6 cut(s) 330, 797, 1008, 1017, 1257, 1315
CaiI CAGNNNCTG 1 cut(s) 907
CciI TCATGA 2 cut(s) 1156, 1237
CfoI GCGC 1 cut(s) 820
Cfr10I RCCGGY 2 cut(s) 770, 1342
Cfr13I GGNCC 5 cut(s) 26, 442, 443, 852, 918
ClaI ATCGAT 1 cut(s) 152
CseI GACGC 3 cut(s) 583, 1346, 1364
Csp6I GTAC 5 cut(s) 49, 774, 788, 1023, 1105
CspAI ACCGGT 1 cut(s) 770
CviQI GTAC 5 cut(s) 49, 774, 788, 1023, 1105
DdeI CTNAG 6 cut(s) 36, 63, 369, 848, 1297, 1321
DraI TTTAAA 1 cut(s) 250
DrdI GACNNNNNNGTC 1 cut(s) 643
DseDI GACNNNNNNGTC 1 cut(s) 643
Eam1104I CTCTTC 3 cut(s) 141, 303, 744
EarI CTCTTC 3 cut(s) 141, 303, 744
EciI GGCGGA 1 cut(s) 741
Eco24I GRGCYC 4 cut(s) 10, 446, 765, 814
Eco47I GGWCC 1 cut(s) 26
Eco47III AGCGCT 1 cut(s) 819
Eco57I CTGAAG 3 cut(s) 725, 771, 804
Eco88I CYCGRG 3 cut(s) 714, 813, 1383
EcoNI CCTNNNNNAGG 1 cut(s) 79
EcoRII CCWGG 2 cut(s) 323, 1308
EcoT22I ATGCAT 1 cut(s) 625
EcoT38I GRGCYC 4 cut(s) 10, 446, 765, 814
FaqI GGGAC 3 cut(s) 12, 1133, 1393
FokI GGATG 2 cut(s) 558, 986
FriOI GRGCYC 4 cut(s) 10, 446, 765, 814
FspBI CTAG 6 cut(s) 168, 177, 234, 417, 560, 1314
GlaI GCGC 1 cut(s) 819
GsuI CTGGAG 1 cut(s) 619
HaeII RGCGCY 1 cut(s) 821
HaeIII GGCC 4 cut(s) 444, 853, 919, 1006
HapII CCGG 4 cut(s) 211, 771, 864, 1343
HgaI GACGC 3 cut(s) 583, 1346, 1364
HhaI GCGC 1 cut(s) 820
Hin6I GCGC 1 cut(s) 818
HinP1I GCGC 1 cut(s) 818
HincII GTYRAC 2 cut(s) 297, 870
HindII GTYRAC 2 cut(s) 297, 870
HinfI GANTC 7 cut(s) 171, 871, 904, 1175, 1234, 1349, 1381
HpaII CCGG 4 cut(s) 211, 771, 864, 1343
HphI GGTGA 4 cut(s) 87, 1061, 1281, 1412
Hpy166II GTNNAC 3 cut(s) 297, 870, 1105
Hpy188III TCNNGA 9 cut(s) 140, 348, 598, 716, 956, 1157, 1238, 1385, 1424
Hpy8I GTNNAC 3 cut(s) 297, 870, 1105
Hpy99I CGWCG 1 cut(s) 1340
HpyAV CCTTC 4 cut(s) 214, 345, 879, 1080
HpyCH4III ACNGT 2 cut(s) 229, 586
HpyCH4IV ACGT 1 cut(s) 92
HpyCH4V TGCA 4 cut(s) 78, 102, 623, 650
HpyF10VI GCNNNNNNNGC 4 cut(s) 647, 656, 1012, 1016
HpyF3I CTNAG 6 cut(s) 36, 63, 369, 848, 1297, 1321
HpySE526I ACGT 1 cut(s) 92
HspAI GCGC 1 cut(s) 818
LmnI GCTCC 2 cut(s) 1306, 1322
LweI GCATC 5 cut(s) 363, 459, 536, 1111, 1219
MaeI CTAG 6 cut(s) 168, 177, 234, 417, 560, 1314
MaeII ACGT 1 cut(s) 92
MaeIII GTNAC 5 cut(s) 93, 229, 634, 709, 1287
MboII GAAGA 9 cut(s) 128, 290, 320, 404, 405, 407, 731, 1130, 1381
MflI RGATCY 2 cut(s) 57, 337
MhlI GDGCHC 4 cut(s) 10, 446, 765, 814
MlyI GAGTC 4 cut(s) 165, 865, 913, 1375
MmeI TCCRAC 2 cut(s) 682, 1359
Mph1103I ATGCAT 1 cut(s) 625
MseI TTAA 1 cut(s) 249
MspI CCGG 4 cut(s) 211, 771, 864, 1343
MspR9I CCNGG 4 cut(s) 211, 325, 865, 1310
Mva1269I GAATGC 1 cut(s) 625
MvaI CCWGG 2 cut(s) 325, 1310
MwoI GCNNNNNNNGC 4 cut(s) 647, 656, 1012, 1016
NciI CCSGG 2 cut(s) 211, 865
NheI GCTAGC 1 cut(s) 1313
NlaIV GGNNCC 2 cut(s) 28, 444
NmeAIII GCCGAG 1 cut(s) 991
NmuCI GTSAC 3 cut(s) 93, 634, 1287
NsiI ATGCAT 1 cut(s) 625
NspI RCATGY 3 cut(s) 332, 799, 847
PaeI GCATGC 2 cut(s) 332, 799
PaeR7I CTCGAG 1 cut(s) 813
PagI TCATGA 2 cut(s) 1156, 1237
PaqCI CACCTGC 1 cut(s) 83
PctI GAATGC 1 cut(s) 625
PfeI GAWTC 3 cut(s) 1175, 1234, 1349
PfoI TCCNGGA 1 cut(s) 209
PinAI ACCGGT 1 cut(s) 770
PleI GAGTC 4 cut(s) 165, 865, 912, 1375
PpsI GAGTC 4 cut(s) 165, 865, 912, 1375
PsiI TTATAA 1 cut(s) 573
Psp6I CCWGG 2 cut(s) 323, 1308
PspGI CCWGG 2 cut(s) 323, 1308
PspN4I GGNNCC 2 cut(s) 28, 444
PspOMI GGGCCC 1 cut(s) 442
PspPI GGNCC 5 cut(s) 26, 442, 443, 852, 918
PspXI VCTCGAGB 1 cut(s) 813
PsrI GAACNNNNNNTAC 2 cut(s) 1264, 1296
PstNI CAGNNNCTG 1 cut(s) 907
PsuI RGATCY 2 cut(s) 57, 337
RsaI GTAC 5 cut(s) 50, 775, 789, 1024, 1106
RsaNI GTAC 5 cut(s) 49, 774, 788, 1023, 1105
SaqAI TTAA 1 cut(s) 249
Sau96I GGNCC 5 cut(s) 26, 442, 443, 852, 918
SchI GAGTC 4 cut(s) 165, 865, 913, 1375
ScrFI CCNGG 4 cut(s) 211, 325, 865, 1310
SduI GDGCHC 4 cut(s) 10, 446, 765, 814
SfaNI GCATC 5 cut(s) 363, 459, 536, 1111, 1219
Sfr274I CTCGAG 1 cut(s) 813
SinI GGWCC 1 cut(s) 26
SlaI CTCGAG 1 cut(s) 813
SmiI ATTTAAAT 1 cut(s) 250
SmlI CTYRAG 2 cut(s) 805, 813
SmoI CTYRAG 2 cut(s) 805, 813
SphI GCATGC 2 cut(s) 332, 799
SsiI CCGC 1 cut(s) 726
SspMI CTAG 6 cut(s) 168, 177, 234, 417, 560, 1314
StyD4I CCNGG 4 cut(s) 209, 323, 863, 1308
SwaI ATTTAAAT 1 cut(s) 250
TaaI ACNGT 2 cut(s) 229, 586
TaiI ACGT 1 cut(s) 95
TaqI TCGA 4 cut(s) 152, 425, 814, 1073
TfiI GAWTC 3 cut(s) 1175, 1234, 1349
Tru1I TTAA 1 cut(s) 249
Tru9I TTAA 1 cut(s) 249
TscAI CASTG 1 cut(s) 832
TseFI GTSAC 3 cut(s) 93, 634, 1287
Tsp45I GTSAC 3 cut(s) 93, 634, 1287
TspDTI ATGAA 8 cut(s) 405, 903, 990, 1145, 1190, 1202, 1254, 1341
TspRI CASTG 1 cut(s) 832
VpaK11BI GGWCC 1 cut(s) 26
XagI CCTNNNNNAGG 1 cut(s) 79
XapI RAATTY 2 cut(s) 180, 964
XceI RCATGY 3 cut(s) 332, 799, 847
XhoI CTCGAG 1 cut(s) 813
XspI CTAG 6 cut(s) 168, 177, 234, 417, 560, 1314
Zsp2I ATGCAT 1 cut(s) 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.