MD15G1362200.v1.1

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006242
Physical Location & Seq
Reverse (-)
43676115 .. 43678994
2880 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1362200.v1.1.491

Sequence Viewer

Length: 1344 bp
ATGTCATCATGTACTTTGGAGGAGAGCCAAAGACTAATTCCCAATCTTCCTGATGAGCTGTCAGTCCAAATTATTGCTAGACTTCCTAGAATTTGCTACTTTGACATCAGGTTGGTTTCACGGAAGTGGAAGGAAACTGTTACAAGCTCCGAACTATTTAAATTGAGAAAAGAAATTGGTAGAAGTGAAGAATGGCTGTACTTGTTGACAAAAATTGAAGAGGATAAACTTTCCTGGCATGCTTTTGATCCCCTGTCAAGAATGTGGCAGAGGCTACCTCAGATGCCCAATGCAGTTTATGAAGAAGAATACAGTAAGGTTTCTTCTAGATTTTCAATGTGGAATATGGTGGGCCCAAGTCTCAAAATTGCTCATACTATTAGAGGCTGGCTAGGGGGAAAGAACTCGTTTGAACAAATGCCATTTTGCGGTTGCGCCATTGGTGCTGTTGATGGATGCCTTTATGTTCTAGGTGGATTTTATAAAACTACGACCATGAGGTGTGTCTGGAGATTTGATCCAATTCAGAATGCATGGAGTGAAGTGACAGCCATGTCTGCTAGTAGGGCCTATTGTAAGACAAGCATTCTGAATAATAAATTGTATGTTGTTGGAGGAGTTAGTCGGGAACAAGGTGGATTGATCCCCCTTCAGTCTGCCGAAGTTTTTGAGCCCTCCACTGGTACGTGGTCCGAAGTACCAAGCATGCCATTCTCAAGGGCTCAAGCCTTGCCCACTGCCTTTTTGACTGACATGCTAAGGCCTATTGCCACTGGGTTGACTTCCTACATGGGTAGACTATGCGTATCCCAGAGTCTGTATTCATGGCCCTTTTTTGTTGATGTTGGGGGAGAAATCTATGATCCCGAGACAAATTCTTGGGATGAAATGCCTCTTGGCATGGGAGAGGGTTGGCCTGCTCGGCAGGCAGGTACAAAGTTGAGTGTTGTGGTAGATGGTGAATTATATGCTTTTGATCCTTCGAGTTCATTGGATAGTGGTAAGATCAAGGTGTACGATCAAGGAGAAGATGCTTGGAAAGTTGTTATAGGAAAAGTCCCTATTCATGACTTTGCTGGTTCAGATTCTCCGTATTTACTTGCTGGTTTTCATGGAAAACTTCATGTCATCACAAAAGATGCGAATCATGAAATCGCAGTTCTGCGTGCTGACCTGTGCAGTAATTTGGGTTCTTTGTCACCAAGATCAACTTCTCTCATGCCTAGCTCCTTGCTTGAACCTTCCGACACTGTGGCAGAATCAGACGCAGTTGTGTGGAAGGTCATTGGGACGAGGGATTTTGGTTCAGGTGAATTGGTTGGTTGTCAAATTCTTGATCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_1

Protein Analysis

448

Amino Acids

49.73

Weight (kDa)

5.28

Isoelectric Point (pI)

49.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 13 - 54 3.4e-08 F-box domain
Kelch_FKB95 PF25210 136 - 380 9.1e-19 FKB95, Kelch-repeats domain
Kelch_1 PF01344 141 - 186 2.4e-12 Kelch motif
Kelch_KLHDC2_KLHL20_DRC7 PF24681 146 - 236 2.4e-08 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_Calicin PF13964 147 - 239 2.1e-07 Calicin, beta-propeller domain
Beta-prop_FBX42 PF13415 151 - 233 3.8e-06 FBX42, beta-propeller domain
Kelch_1 PF01344 189 - 237 6.2e-11 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 483
AasI GACNNNNNNGTC 1 cut(s) 553
Acc36I ACCTGC 1 cut(s) 920
AccI GTMKAC 1 cut(s) 796
AciI CCGC 1 cut(s) 429
AclWI GGATC 5 cut(s) 242, 512, 637, 857, 971
AcsI RAATTY 3 cut(s) 90, 874, 1329
AcuI CTGAAG 1 cut(s) 635
AfaI GTAC 6 cut(s) 13, 200, 685, 699, 934, 1016
AfiI CCNNNNNNNGG 2 cut(s) 428, 680
AgsI TTSAA 4 cut(s) 218, 336, 413, 1238
AjnI CCWGG 1 cut(s) 233
AjuI GAANNNNNNNTTGG 6 cut(s) 21, 53, 879, 911, 1195, 1227
AleI CACNNNNGTG 1 cut(s) 124
AluBI AGCT 3 cut(s) 58, 147, 1227
AluI AGCT 3 cut(s) 58, 147, 1227
Alw26I GTCTC 2 cut(s) 365, 863
AlwI GGATC 5 cut(s) 242, 512, 637, 857, 971
AlwNI CAGNNNCTG 1 cut(s) 817
Ama87I CYCGRG 1 cut(s) 866
AoxI GGCC 5 cut(s) 352, 567, 761, 827, 914
ApaI GGGCCC 1 cut(s) 356
ApoI RAATTY 3 cut(s) 90, 874, 1329
AspLEI GCGC 1 cut(s) 437
AspS9I GGNCC 5 cut(s) 352, 353, 567, 690, 828
AsuHPI GGTGA 3 cut(s) 971, 1191, 1322
AvaI CYCGRG 1 cut(s) 866
AvaII GGWCC 1 cut(s) 690
BaeGI GKGCMC 1 cut(s) 356
BanII GRGCYC 3 cut(s) 356, 675, 724
BarI GAAGNNNNNNTAC 2 cut(s) 307, 339
BccI CCATC 2 cut(s) 446, 950
BciT130I CCWGG 1 cut(s) 235
BciVI GTATCC 1 cut(s) 817
BcoDI GTCTC 2 cut(s) 365, 863
BfaI CTAG 7 cut(s) 78, 87, 327, 392, 470, 561, 1224
BfuAI ACCTGC 1 cut(s) 920
BfuI GTATCC 1 cut(s) 817
BglI GCCNNNNNGGC 1 cut(s) 922
Bme1390I CCNGG 1 cut(s) 235
Bme18I GGWCC 1 cut(s) 690
BmeT110I CYCGRG 1 cut(s) 866
BmgT120I GGNCC 5 cut(s) 352, 353, 567, 690, 828
BmiI GGNNCC 1 cut(s) 354
BmrFI CCNGG 1 cut(s) 235
BmrI ACTGGG 1 cut(s) 783
BmsI GCATC 4 cut(s) 273, 446, 1021, 1129
BmuI ACTGGG 1 cut(s) 783
BplI GAGNNNNNCTC 2 cut(s) 262, 294
BpmI CTGGAG 1 cut(s) 529
Bpu10I CCTNAGC 1 cut(s) 758
BpuEI CTTGAG 2 cut(s) 700, 708
BsaAI YACGTR 1 cut(s) 687
BsaBI GATNNNNATC 1 cut(s) 1143
Bsc4I CCNNNNNNNGG 2 cut(s) 428, 680
Bse1I ACTGG 2 cut(s) 685, 778
Bse8I GATNNNNATC 1 cut(s) 1143
BseBI CCWGG 1 cut(s) 235
BseGI GGATG 2 cut(s) 461, 889
BseJI GATNNNNATC 1 cut(s) 1143
BseLI CCNNNNNNNGG 2 cut(s) 428, 680
BseMII CTCAG 1 cut(s) 293
BseNI ACTGG 2 cut(s) 685, 778
BseRI GAGGAG 2 cut(s) 35, 630
BseSI GKGCMC 1 cut(s) 356
BsgI GTGCAG 1 cut(s) 1198
BshFI GGCC 5 cut(s) 354, 569, 763, 829, 916
BsiHKCI CYCGRG 1 cut(s) 866
BslFI GGGAC 2 cut(s) 1043, 1303
BslI CCNNNNNNNGG 2 cut(s) 428, 680
BsmAI GTCTC 2 cut(s) 365, 863
BsmFI GGGAC 2 cut(s) 1043, 1303
BsmI GAATGC 2 cut(s) 535, 585
BsnI GGCC 5 cut(s) 354, 569, 763, 829, 916
BsoBI CYCGRG 1 cut(s) 866
Bsp120I GGGCCC 1 cut(s) 352
Bsp1286I GDGCHC 3 cut(s) 356, 675, 724
Bsp143I GATC 9 cut(s) 247, 517, 642, 862, 976, 1005, 1018, 1205, 1336
BspACI CCGC 1 cut(s) 429
BspANI GGCC 5 cut(s) 354, 569, 763, 829, 916
BspCNI CTCAG 1 cut(s) 292
BspHI TCATGA 2 cut(s) 1066, 1147
BspLI GGNNCC 1 cut(s) 354
BspMI ACCTGC 1 cut(s) 920
BspPI GGATC 5 cut(s) 242, 512, 637, 857, 971
BsrI ACTGG 2 cut(s) 685, 778
BssMI GATC 9 cut(s) 247, 517, 642, 862, 976, 1005, 1018, 1205, 1336
Bst2UI CCWGG 1 cut(s) 235
Bst4CI ACNGT 3 cut(s) 139, 314, 1252
Bst6I CTCTTC 1 cut(s) 213
BstBAI YACGTR 1 cut(s) 687
BstC8I GCNNGC 6 cut(s) 240, 389, 707, 918, 927, 1167
BstDEI CTNAG 2 cut(s) 279, 758
BstF5I GGATG 2 cut(s) 461, 889
BstHHI GCGC 1 cut(s) 437
BstKTI GATC 9 cut(s) 250, 520, 645, 865, 979, 1008, 1021, 1208, 1339
BstMAI GTCTC 2 cut(s) 365, 863
BstMBI GATC 9 cut(s) 247, 517, 642, 862, 976, 1005, 1018, 1205, 1336
BstMWI GCNNNNNNNGC 5 cut(s) 443, 557, 566, 922, 926
BstNI CCWGG 1 cut(s) 235
BstNSI RCATGY 3 cut(s) 242, 709, 757
BstSCI CCNGG 1 cut(s) 233
BstSLI GKGCMC 1 cut(s) 356
BsuI GTATCC 1 cut(s) 817
BsuRI GGCC 5 cut(s) 354, 569, 763, 829, 916
BtsCI GGATG 2 cut(s) 461, 889
BtsI GCAGTG 1 cut(s) 735
BtsIMutI CAGTG 4 cut(s) 678, 735, 771, 1248
BveI ACCTGC 1 cut(s) 920
Cac8I GCNNGC 6 cut(s) 240, 389, 707, 918, 927, 1167
CaiI CAGNNNCTG 1 cut(s) 817
CciI TCATGA 2 cut(s) 1066, 1147
CfoI GCGC 1 cut(s) 437
Cfr13I GGNCC 5 cut(s) 352, 353, 567, 690, 828
CseI GACGC 1 cut(s) 1274
Csp6I GTAC 6 cut(s) 12, 199, 684, 698, 933, 1015
CviQI GTAC 6 cut(s) 12, 199, 684, 698, 933, 1015
DdeI CTNAG 2 cut(s) 279, 758
DpnI GATC 9 cut(s) 249, 519, 644, 864, 978, 1007, 1020, 1207, 1338
DpnII GATC 9 cut(s) 247, 517, 642, 862, 976, 1005, 1018, 1205, 1336
DraI TTTAAA 1 cut(s) 160
DrdI GACNNNNNNGTC 1 cut(s) 553
DseDI GACNNNNNNGTC 1 cut(s) 553
Eam1104I CTCTTC 1 cut(s) 213
EarI CTCTTC 1 cut(s) 213
Eco147I AGGCCT 1 cut(s) 763
Eco24I GRGCYC 3 cut(s) 356, 675, 724
Eco47I GGWCC 1 cut(s) 690
Eco57I CTGAAG 1 cut(s) 635
Eco88I CYCGRG 1 cut(s) 866
EcoO109I RGGNCCY 1 cut(s) 567
EcoRII CCWGG 1 cut(s) 233
EcoT22I ATGCAT 1 cut(s) 535
EcoT38I GRGCYC 3 cut(s) 356, 675, 724
FalI AAGNNNNNCTT 2 cut(s) 1195, 1227
FaqI GGGAC 2 cut(s) 1043, 1303
FblI GTMKAC 1 cut(s) 796
FokI GGATG 2 cut(s) 468, 896
FriOI GRGCYC 3 cut(s) 356, 675, 724
FspBI CTAG 7 cut(s) 78, 87, 327, 392, 470, 561, 1224
GlaI GCGC 1 cut(s) 436
GsuI CTGGAG 1 cut(s) 529
HaeIII GGCC 5 cut(s) 354, 569, 763, 829, 916
HgaI GACGC 1 cut(s) 1274
HhaI GCGC 1 cut(s) 437
Hin6I GCGC 1 cut(s) 435
HinP1I GCGC 1 cut(s) 435
HincII GTYRAC 2 cut(s) 207, 780
HindII GTYRAC 2 cut(s) 207, 780
HinfI GANTC 4 cut(s) 814, 1085, 1144, 1259
HphI GGTGA 3 cut(s) 971, 1191, 1322
Hpy166II GTNNAC 4 cut(s) 207, 780, 797, 1015
Hpy188I TCNGA 8 cut(s) 151, 282, 528, 591, 694, 1084, 1246, 1264
Hpy188III TCNNGA 9 cut(s) 50, 258, 327, 508, 626, 866, 1067, 1148, 1334
Hpy8I GTNNAC 4 cut(s) 207, 780, 797, 1015
HpyAV CCTTC 5 cut(s) 124, 659, 990, 1251, 1273
HpyCH4III ACNGT 3 cut(s) 139, 314, 1252
HpyCH4IV ACGT 1 cut(s) 686
HpyCH4V TGCA 3 cut(s) 293, 533, 1179
HpyF10VI GCNNNNNNNGC 5 cut(s) 443, 557, 566, 922, 926
HpyF3I CTNAG 2 cut(s) 279, 758
HpySE526I ACGT 1 cut(s) 686
HspAI GCGC 1 cut(s) 435
Kzo9I GATC 9 cut(s) 247, 517, 642, 862, 976, 1005, 1018, 1205, 1336
LmnI GCTCC 2 cut(s) 152, 1232
LweI GCATC 4 cut(s) 273, 446, 1021, 1129
MaeI CTAG 7 cut(s) 78, 87, 327, 392, 470, 561, 1224
MaeII ACGT 1 cut(s) 686
MaeIII GTNAC 3 cut(s) 139, 544, 1197
MalI GATC 9 cut(s) 249, 519, 644, 864, 978, 1007, 1020, 1207, 1338
MboI GATC 9 cut(s) 247, 517, 642, 862, 976, 1005, 1018, 1205, 1336
MboII GAAGA 7 cut(s) 38, 200, 230, 314, 315, 317, 1040
MhlI GDGCHC 3 cut(s) 356, 675, 724
MlyI GAGTC 1 cut(s) 823
MmeI TCCRAC 2 cut(s) 592, 1269
Mph1103I ATGCAT 1 cut(s) 535
MseI TTAA 1 cut(s) 159
MslI CAYNNNNRTG 1 cut(s) 124
MspR9I CCNGG 1 cut(s) 235
Mva1269I GAATGC 2 cut(s) 535, 585
MvaI CCWGG 1 cut(s) 235
MwoI GCNNNNNNNGC 5 cut(s) 443, 557, 566, 922, 926
NdeII GATC 9 cut(s) 247, 517, 642, 862, 976, 1005, 1018, 1205, 1336
NlaIV GGNNCC 1 cut(s) 354
NmeAIII GCCGAG 1 cut(s) 901
NmuCI GTSAC 2 cut(s) 544, 1197
NsiI ATGCAT 1 cut(s) 535
NspI RCATGY 3 cut(s) 242, 709, 757
OliI CACNNNNGTG 1 cut(s) 124
PaeI GCATGC 2 cut(s) 242, 709
PagI TCATGA 2 cut(s) 1066, 1147
PceI AGGCCT 1 cut(s) 763
PctI GAATGC 2 cut(s) 535, 585
PfeI GAWTC 3 cut(s) 1085, 1144, 1259
PleI GAGTC 1 cut(s) 822
PpsI GAGTC 1 cut(s) 822
Ppu21I YACGTR 1 cut(s) 687
PsiI TTATAA 1 cut(s) 483
Psp6I CCWGG 1 cut(s) 233
PspGI CCWGG 1 cut(s) 233
PspN4I GGNNCC 1 cut(s) 354
PspOMI GGGCCC 1 cut(s) 352
PspPI GGNCC 5 cut(s) 352, 353, 567, 690, 828
PsrI GAACNNNNNNTAC 2 cut(s) 1174, 1206
PstNI CAGNNNCTG 1 cut(s) 817
RsaI GTAC 6 cut(s) 13, 200, 685, 699, 934, 1016
RsaNI GTAC 6 cut(s) 12, 199, 684, 698, 933, 1015
RseI CAYNNNNRTG 1 cut(s) 124
SaqAI TTAA 1 cut(s) 159
Sau3AI GATC 9 cut(s) 247, 517, 642, 862, 976, 1005, 1018, 1205, 1336
Sau96I GGNCC 5 cut(s) 352, 353, 567, 690, 828
SchI GAGTC 1 cut(s) 823
ScrFI CCNGG 1 cut(s) 235
SduI GDGCHC 3 cut(s) 356, 675, 724
SfaNI GCATC 4 cut(s) 273, 446, 1021, 1129
SinI GGWCC 1 cut(s) 690
SmiI ATTTAAAT 1 cut(s) 160
SmiMI CAYNNNNRTG 1 cut(s) 124
SmlI CTYRAG 2 cut(s) 715, 723
SmoI CTYRAG 2 cut(s) 715, 723
SphI GCATGC 2 cut(s) 242, 709
SseBI AGGCCT 1 cut(s) 763
SsiI CCGC 1 cut(s) 429
SspMI CTAG 7 cut(s) 78, 87, 327, 392, 470, 561, 1224
StuI AGGCCT 1 cut(s) 763
StyD4I CCNGG 1 cut(s) 233
SwaI ATTTAAAT 1 cut(s) 160
TaaI ACNGT 3 cut(s) 139, 314, 1252
TaiI ACGT 1 cut(s) 689
TaqI TCGA 1 cut(s) 983
TatI WGTACW 2 cut(s) 11, 198
TfiI GAWTC 3 cut(s) 1085, 1144, 1259
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TscAI CASTG 4 cut(s) 685, 742, 778, 1255
TseFI GTSAC 2 cut(s) 544, 1197
Tsp45I GTSAC 2 cut(s) 544, 1197
TspDTI ATGAA 8 cut(s) 315, 813, 900, 978, 1055, 1100, 1112, 1164
TspGWI ACGGA 2 cut(s) 136, 1080
TspRI CASTG 4 cut(s) 685, 742, 778, 1255
VpaK11BI GGWCC 1 cut(s) 690
XapI RAATTY 3 cut(s) 90, 874, 1329
XbaI TCTAGA 1 cut(s) 326
XceI RCATGY 3 cut(s) 242, 709, 757
XmiI GTMKAC 1 cut(s) 796
XspI CTAG 7 cut(s) 78, 87, 327, 392, 470, 561, 1224
Zsp2I ATGCAT 1 cut(s) 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.