Rh7BG460600

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0006242
Physical Location & Seq
Forward (+)
54587207 .. 54590957
3751 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG460600.1

Sequence Viewer

Length: 1437 bp
ATGGGGGCTATTTTGAGTTTGGCTGGTCCCAAATCTAGGACAAACGATTATCATGAGGTTTCTCAGAATGATACCTGTAAGCGGCAAAGAATGTCATCATGTACTTATGAGGAGAGCAAAAGACTAATTCCCAATCTTCCTGATGAGCTCTCAATCCAAATTATTGCTAGACTTCCTAGAATTTGCTACCCCCATATCAGGCTGGTGTCACAGAAGTGGAAGGAAACTGTTACAAGCTCTGAACTATTTAAGTTGAGAAAAGAGCTTGGAAAAACAGAAGAATGGCTGTACTTGTTGACTAAAATTGAAGAGGATAAACTCTCGTGGCATGCTTTTGACCCCCTGTCAAGGAAGTGGCAGAGGCTACCTCGGATGCCAAACGTAGTTTATGAAGAAGAGTATACTAGAGGTTTGCCTTGGTTTTCGATATGGAATATGGTTGGGCCAAGCATCAAAATTGCTGATACTATTAGAGGCTGGCTTGGGAGAAAGAACTCATTTGATCAAATGCCATTTTGTGGTTGTGCCATTGGTGCTCTTGATGGATGCCTCTATGTTCTAGGTGGATTCTGTAAAACTTCAACTATGAAATGTGTGTGGAGATTTGATCCAATTCAAAATGCATGGAGCGAAGTGCCAGCCATGTCCACAAGTAGAGCCTATGGTAAGACGGGCATTCTGAATAACAAGCTCTATGTCGTTGGAGGGGTTAGTCGAGGCCAAGGTGGATTGGCTCCTCTTCAATCTGCTGAAGTTTTTGACCCTTCCACAGGTACGTGGTCCGAAGTACCAAGCATGCCATTCTCAAAAGCTCAAGCCTTGCCTACTGCCTTCTTGACTGACATGCTAAAGCCTATTGCCACCGGGTTGACTACATACATGGGAAGGTTATGTGTATCCCAGAGTCTGTATTCATGGCCCTTTTTTGTTGATGTTGGAGGAGAAATTTATGATCCTGAGACAAATTCATGGATTGAAATGCCAAATGGCATGGGTGACGGTTGGCCTGCTCGGCAAGCTGGTACGAAGTTGAGTGTTGTGGTAGATGGTGAATTGTATGCTTTTGATCCTTCGAGTTCTTTGGATAGTGGCAAGATTAAGGTGTATGATCAACAGGAAGATGCTTGGAAAGTTGTTATACGGAAAGTCCCCATGTGCGATTTGGCTGGTTCAGAATCTCCATATTTACTTGCTGGTTTTCATGGAAAGCTTCATGTTATAACAAAAGATATTAATCATGATATCGCTGTTCTGCGGGCTGATCTGCGGAACAATTTGGGTTCACTGTCGTCAAGCTCATGTTCTGTCGTAGCCGGATCCTTATTACATGAACATCCCGAGTCACTGGCAGAATCAGATACAGTTGTGTGGAAAGCCATTGGCACTAAGGATTTTGGGTCAAGTGAATTGGTCAGTTGTCAAGTTATTGACATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_1

Protein Analysis

478

Amino Acids

53.21

Weight (kDa)

6.28

Isoelectric Point (pI)

47.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 43 - 84 3e-08 F-box domain
Kelch_FKB95 PF25210 164 - 380 2.4e-18 FKB95, Kelch-repeats domain
Kelch_1 PF01344 171 - 216 7.8e-13 Kelch motif
Beta-prop_Calicin PF13964 178 - 237 1.1e-06 Calicin, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 179 - 266 4.9e-08 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_1 PF01344 219 - 267 8.4e-12 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1220
AccB7I CCANNNNNTGG 1 cut(s) 518
AccI GTMKAC 1 cut(s) 401
AciI CCGC 3 cut(s) 82, 1255, 1267
AclWI GGATC 5 cut(s) 602, 947, 1061, 1311, 1324
AcsI RAATTY 3 cut(s) 180, 945, 964
AcuI CTGAAG 1 cut(s) 771
AfaI GTAC 5 cut(s) 103, 290, 775, 789, 1024
AfiI CCNNNNNNNGG 6 cut(s) 36, 81, 198, 348, 518, 770
AgsI TTSAA 5 cut(s) 308, 582, 617, 743, 977
AhdI GACNNNNNGTC 1 cut(s) 343
AleI CACNNNNGTG 1 cut(s) 214
AluBI AGCT 8 cut(s) 148, 237, 265, 691, 812, 1019, 1210, 1296
AluI AGCT 8 cut(s) 148, 237, 265, 691, 812, 1019, 1210, 1296
Alw21I GWGCWC 2 cut(s) 150, 538
Alw26I GTCTC 1 cut(s) 953
AlwI GGATC 5 cut(s) 602, 947, 1061, 1311, 1324
AlwNI CAGNNNCTG 1 cut(s) 907
Ama87I CYCGRG 1 cut(s) 1337
AoxI GGCC 4 cut(s) 443, 718, 917, 1004
ApoI RAATTY 3 cut(s) 180, 945, 964
AseI ATTAAT 1 cut(s) 1233
AspS9I GGNCC 4 cut(s) 26, 443, 780, 918
AsuC2I CCSGG 1 cut(s) 865
AsuHPI GGTGA 2 cut(s) 1007, 1061
AvaI CYCGRG 1 cut(s) 1337
AvaII GGWCC 2 cut(s) 26, 780
BaeI ACNNNNGTAYC 2 cut(s) 1350, 1383
BamHI GGATCC 1 cut(s) 1316
BanII GRGCYC 1 cut(s) 150
BauI CACGAG 1 cut(s) 322
Bbv12I GWGCWC 2 cut(s) 150, 538
BccI CCATC 2 cut(s) 536, 1040
BciVI GTATCC 1 cut(s) 907
BclI TGATCA 2 cut(s) 502, 1108
BcnI CCSGG 1 cut(s) 865
BcoDI GTCTC 1 cut(s) 953
BfaI CTAG 5 cut(s) 36, 168, 177, 405, 560
BfuI GTATCC 1 cut(s) 907
BglI GCCNNNNNGGC 1 cut(s) 1012
BisI GCNGC 1 cut(s) 83
BlsI GCNGC 1 cut(s) 84
Bme1390I CCNGG 1 cut(s) 865
Bme18I GGWCC 2 cut(s) 26, 780
BmeRI GACNNNNNGTC 1 cut(s) 343
BmeT110I CYCGRG 1 cut(s) 1337
BmgT120I GGNCC 4 cut(s) 26, 443, 780, 918
BmiI GGNNCC 3 cut(s) 28, 735, 1318
BmrFI CCNGG 1 cut(s) 865
BmsI GCATC 4 cut(s) 363, 459, 536, 1111
BplI GAGNNNNNCTC 2 cut(s) 352, 384
BpuEI CTTGAG 1 cut(s) 798
BpuMI CCSGG 1 cut(s) 865
BsaAI YACGTR 1 cut(s) 777
BsaBI GATNNNNATC 1 cut(s) 1233
BsaJI CCNNGG 3 cut(s) 368, 416, 721
Bsc4I CCNNNNNNNGG 6 cut(s) 36, 81, 198, 348, 518, 770
Bse1I ACTGG 1 cut(s) 1350
Bse8I GATNNNNATC 1 cut(s) 1233
BseDI CCNNGG 3 cut(s) 368, 416, 721
BseGI GGATG 3 cut(s) 378, 551, 1333
BseJI GATNNNNATC 1 cut(s) 1233
BseLI CCNNNNNNNGG 6 cut(s) 36, 81, 198, 348, 518, 770
BseMII CTCAG 2 cut(s) 77, 948
BseNI ACTGG 1 cut(s) 1350
BseRI GAGGAG 3 cut(s) 125, 726, 954
BshFI GGCC 4 cut(s) 445, 720, 919, 1006
BsiHKAI GWGCWC 2 cut(s) 150, 538
BsiHKCI CYCGRG 1 cut(s) 1337
BsiSI CCGG 2 cut(s) 864, 1314
BslFI GGGAC 2 cut(s) 12, 1133
BslI CCNNNNNNNGG 6 cut(s) 36, 81, 198, 348, 518, 770
BsmAI GTCTC 1 cut(s) 953
BsmFI GGGAC 2 cut(s) 12, 1133
BsmI GAATGC 1 cut(s) 675
BsnI GGCC 4 cut(s) 445, 720, 919, 1006
BsoBI CYCGRG 1 cut(s) 1337
Bsp1286I GDGCHC 2 cut(s) 150, 538
Bsp143I GATC 7 cut(s) 502, 607, 952, 1066, 1108, 1261, 1316
BspACI CCGC 3 cut(s) 82, 1255, 1267
BspANI GGCC 4 cut(s) 445, 720, 919, 1006
BspCNI CTCAG 2 cut(s) 76, 949
BspHI TCATGA 2 cut(s) 52, 1237
BspLI GGNNCC 3 cut(s) 28, 735, 1318
BspPI GGATC 5 cut(s) 602, 947, 1061, 1311, 1324
BsrI ACTGG 1 cut(s) 1350
BssECI CCNNGG 3 cut(s) 368, 416, 721
BssMI GATC 7 cut(s) 502, 607, 952, 1066, 1108, 1261, 1316
BssNAI GTATAC 1 cut(s) 402
BssSI CACGAG 1 cut(s) 322
BssT1I CCWWGG 2 cut(s) 416, 721
Bst1107I GTATAC 1 cut(s) 402
Bst2BI CACGAG 1 cut(s) 322
Bst4CI ACNGT 4 cut(s) 229, 1001, 1287, 1363
Bst6I CTCTTC 3 cut(s) 303, 390, 744
BstBAI YACGTR 1 cut(s) 777
BstC8I GCNNGC 7 cut(s) 330, 479, 639, 797, 1008, 1017, 1257
BstDEI CTNAG 3 cut(s) 63, 957, 1386
BstENI CCTNNNNNAGG 1 cut(s) 768
BstF5I GGATG 3 cut(s) 378, 551, 1333
BstKTI GATC 7 cut(s) 505, 610, 955, 1069, 1111, 1264, 1319
BstMAI GTCTC 1 cut(s) 953
BstMBI GATC 7 cut(s) 502, 607, 952, 1066, 1108, 1261, 1316
BstMWI GCNNNNNNNGC 3 cut(s) 533, 1012, 1016
BstNSI RCATGY 3 cut(s) 332, 799, 847
BstSCI CCNGG 1 cut(s) 863
BstX2I RGATCY 1 cut(s) 1316
BstYI RGATCY 1 cut(s) 1316
BstZ17I GTATAC 1 cut(s) 402
BsuI GTATCC 1 cut(s) 907
BsuRI GGCC 4 cut(s) 445, 720, 919, 1006
BtsCI GGATG 3 cut(s) 378, 551, 1333
BtsIMutI CAGTG 2 cut(s) 1283, 1343
Cac8I GCNNGC 7 cut(s) 330, 479, 639, 797, 1008, 1017, 1257
CaiI CAGNNNCTG 1 cut(s) 907
CciI TCATGA 2 cut(s) 52, 1237
Cfr13I GGNCC 4 cut(s) 26, 443, 780, 918
Csp6I GTAC 5 cut(s) 102, 289, 774, 788, 1023
CviQI GTAC 5 cut(s) 102, 289, 774, 788, 1023
DdeI CTNAG 3 cut(s) 63, 957, 1386
DpnI GATC 7 cut(s) 504, 609, 954, 1068, 1110, 1263, 1318
DpnII GATC 7 cut(s) 502, 607, 952, 1066, 1108, 1261, 1316
DriI GACNNNNNGTC 1 cut(s) 343
Eam1104I CTCTTC 3 cut(s) 303, 390, 744
Eam1105I GACNNNNNGTC 1 cut(s) 343
EarI CTCTTC 3 cut(s) 303, 390, 744
Ecl136II GAGCTC 1 cut(s) 148
Eco130I CCWWGG 2 cut(s) 416, 721
Eco24I GRGCYC 1 cut(s) 150
Eco32I GATATC 1 cut(s) 1243
Eco47I GGWCC 2 cut(s) 26, 780
Eco53kI GAGCTC 1 cut(s) 148
Eco57I CTGAAG 1 cut(s) 771
Eco88I CYCGRG 1 cut(s) 1337
EcoICRI GAGCTC 1 cut(s) 148
EcoNI CCTNNNNNAGG 1 cut(s) 768
EcoRV GATATC 1 cut(s) 1243
EcoT14I CCWWGG 2 cut(s) 416, 721
EcoT22I ATGCAT 1 cut(s) 625
EcoT38I GRGCYC 1 cut(s) 150
ErhI CCWWGG 2 cut(s) 416, 721
FaqI GGGAC 2 cut(s) 12, 1133
FauI CCCGC 1 cut(s) 1248
FbaI TGATCA 2 cut(s) 502, 1108
FblI GTMKAC 1 cut(s) 401
Fnu4HI GCNGC 1 cut(s) 83
FokI GGATG 3 cut(s) 385, 558, 1320
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 1 cut(s) 83
FspBI CTAG 5 cut(s) 36, 168, 177, 405, 560
GluI GCNGC 1 cut(s) 83
HaeIII GGCC 4 cut(s) 445, 720, 919, 1006
HapII CCGG 2 cut(s) 864, 1314
HincII GTYRAC 2 cut(s) 297, 870
HindII GTYRAC 2 cut(s) 297, 870
HindIII AAGCTT 1 cut(s) 1208
HinfI GANTC 5 cut(s) 567, 904, 1175, 1340, 1352
HpaII CCGG 2 cut(s) 864, 1314
HphI GGTGA 2 cut(s) 1007, 1061
Hpy166II GTNNAC 5 cut(s) 297, 402, 648, 870, 1283
Hpy188I TCNGA 7 cut(s) 66, 241, 372, 681, 784, 1174, 1357
Hpy188III TCNNGA 7 cut(s) 53, 140, 539, 835, 956, 1238, 1337
Hpy8I GTNNAC 5 cut(s) 297, 402, 648, 870, 1283
HpyAV CCTTC 5 cut(s) 214, 774, 841, 879, 1080
HpyCH4III ACNGT 4 cut(s) 229, 1001, 1287, 1363
HpyCH4IV ACGT 2 cut(s) 381, 776
HpyCH4V TGCA 1 cut(s) 623
HpyF10VI GCNNNNNNNGC 3 cut(s) 533, 1012, 1016
HpyF3I CTNAG 3 cut(s) 63, 957, 1386
HpySE526I ACGT 2 cut(s) 381, 776
Ksp22I TGATCA 2 cut(s) 502, 1108
Kzo9I GATC 7 cut(s) 502, 607, 952, 1066, 1108, 1261, 1316
LmnI GCTCC 2 cut(s) 627, 739
LweI GCATC 4 cut(s) 363, 459, 536, 1111
MaeI CTAG 5 cut(s) 36, 168, 177, 405, 560
MaeII ACGT 2 cut(s) 381, 776
MaeIII GTNAC 4 cut(s) 207, 229, 995, 1341
MalI GATC 7 cut(s) 504, 609, 954, 1068, 1110, 1263, 1318
MboI GATC 7 cut(s) 502, 607, 952, 1066, 1108, 1261, 1316
MboII GAAGA 7 cut(s) 128, 290, 320, 404, 407, 731, 1130
MflI RGATCY 1 cut(s) 1316
MhlI GDGCHC 2 cut(s) 150, 538
MlyI GAGTC 2 cut(s) 913, 1349
MmeI TCCRAC 2 cut(s) 682, 916
Mph1103I ATGCAT 1 cut(s) 625
MseI TTAA 3 cut(s) 249, 1098, 1233
MslI CAYNNNNRTG 1 cut(s) 214
MspI CCGG 2 cut(s) 864, 1314
MspR9I CCNGG 1 cut(s) 865
Mva1269I GAATGC 1 cut(s) 675
MwoI GCNNNNNNNGC 3 cut(s) 533, 1012, 1016
NciI CCSGG 1 cut(s) 865
NdeII GATC 7 cut(s) 502, 607, 952, 1066, 1108, 1261, 1316
NlaIV GGNNCC 3 cut(s) 28, 735, 1318
NmeAIII GCCGAG 1 cut(s) 991
NmuCI GTSAC 3 cut(s) 207, 995, 1341
NsiI ATGCAT 1 cut(s) 625
NspI RCATGY 3 cut(s) 332, 799, 847
OliI CACNNNNGTG 1 cut(s) 214
PaeI GCATGC 2 cut(s) 332, 799
PagI TCATGA 2 cut(s) 52, 1237
PctI GAATGC 1 cut(s) 675
PfeI GAWTC 3 cut(s) 567, 1175, 1352
PflMI CCANNNNNTGG 1 cut(s) 518
PkrI GCNGC 1 cut(s) 84
PleI GAGTC 2 cut(s) 912, 1348
PpsI GAGTC 2 cut(s) 912, 1348
Ppu21I YACGTR 1 cut(s) 777
PshBI ATTAAT 1 cut(s) 1233
PsiI TTATAA 1 cut(s) 1220
Psp124BI GAGCTC 1 cut(s) 150
PspN4I GGNNCC 3 cut(s) 28, 735, 1318
PspPI GGNCC 4 cut(s) 26, 443, 780, 918
PstNI CAGNNNCTG 1 cut(s) 907
PsuI RGATCY 1 cut(s) 1316
RsaI GTAC 5 cut(s) 103, 290, 775, 789, 1024
RsaNI GTAC 5 cut(s) 102, 289, 774, 788, 1023
RseI CAYNNNNRTG 1 cut(s) 214
SacI GAGCTC 1 cut(s) 150
SaqAI TTAA 3 cut(s) 249, 1098, 1233
SatI GCNGC 1 cut(s) 83
Sau3AI GATC 7 cut(s) 502, 607, 952, 1066, 1108, 1261, 1316
Sau96I GGNCC 4 cut(s) 26, 443, 780, 918
SchI GAGTC 2 cut(s) 913, 1349
ScrFI CCNGG 1 cut(s) 865
SduI GDGCHC 2 cut(s) 150, 538
SfaNI GCATC 4 cut(s) 363, 459, 536, 1111
SinI GGWCC 2 cut(s) 26, 780
SmiMI CAYNNNNRTG 1 cut(s) 214
SmlI CTYRAG 1 cut(s) 813
SmoI CTYRAG 1 cut(s) 813
SphI GCATGC 2 cut(s) 332, 799
SsiI CCGC 3 cut(s) 82, 1255, 1267
SspMI CTAG 5 cut(s) 36, 168, 177, 405, 560
SstI GAGCTC 1 cut(s) 150
StyD4I CCNGG 1 cut(s) 863
StyI CCWWGG 2 cut(s) 416, 721
TaaI ACNGT 4 cut(s) 229, 1001, 1287, 1363
TaiI ACGT 2 cut(s) 384, 779
TaqI TCGA 3 cut(s) 425, 715, 1073
TatI WGTACW 2 cut(s) 101, 288
TauI GCSGC 1 cut(s) 85
TfiI GAWTC 3 cut(s) 567, 1175, 1352
Tru1I TTAA 3 cut(s) 249, 1098, 1233
Tru9I TTAA 3 cut(s) 249, 1098, 1233
TscAI CASTG 2 cut(s) 1290, 1350
TseFI GTSAC 3 cut(s) 207, 995, 1341
Tsp45I GTSAC 3 cut(s) 207, 995, 1341
TspDTI ATGAA 7 cut(s) 405, 602, 903, 957, 1190, 1202, 1344
TspGWI ACGGA 1 cut(s) 1156
TspRI CASTG 2 cut(s) 1290, 1350
Van91I CCANNNNNTGG 1 cut(s) 518
VpaK11BI GGWCC 2 cut(s) 26, 780
VspI ATTAAT 1 cut(s) 1233
XagI CCTNNNNNAGG 1 cut(s) 768
XapI RAATTY 3 cut(s) 180, 945, 964
XceI RCATGY 3 cut(s) 332, 799, 847
XcmI CCANNNNNNNNNTGG 1 cut(s) 1159
XmiI GTMKAC 1 cut(s) 401
XspI CTAG 5 cut(s) 36, 168, 177, 405, 560
Zsp2I ATGCAT 1 cut(s) 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.