pycom08g15140

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0006242
Physical Location & Seq
Reverse (-)
14600560 .. 14601903
1344 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g15140.1

Sequence Viewer

Length: 1344 bp
ATGTCACCATGTACTTTTGAGGAGAGCCAAAGACTAATTCCCAATCTTCCTGATGAGCTATCGATCCAAATTATTGCCAGACTCCCTAGAATTTGCTACTTCAATATCAGGCTGGTTTCCCGGAAGTGGAAGGAAACTGTTACTAGCTCTGAACTATTTAAATTGAGAAAAGAACTTGGTAGAAGTGAAGAATGGCTGTACTTGTTGACAAAAATTGAAGAGGATAAACTTTCCTGGCATGCTTTCGATCCCCTGTCGAGAAGGTGGCAGAGGCTACCTCAGATGCCCAATGTAGTTTATGAAGAAGAGTTCAGCAAGAGTTCTTCTAGGTTTTCGATGTGGAATATGGTGGGCCCAAGTATCAAAATTGCTCATACTATTAGAGGCTGGTTAGGGGGAAAGAACGCATTTGAACAAATGCCGTTTTGTGGTTGTGCCATTGGTGCTGTTGATGGATGCCTTTATGTTCTAGGTGGATTTTATAAAGCTACGACCGTGAGGTGCGTCTGGAGATTTGATCCAATTCAGAATGCATGGAGTGAAGTGACAGCCATGTCTGCAAGTAGAGCCTATTGTAAGACAGGTATTCTGAATAACAAACTTTATGTTGTTGGAGGGGTTACTCGGGAACGAGGTGGATTGATCCCTCTTCAGTCTGCTGAAGTTTTTGAGCCCTCCACTGGTACATGGTCTGAAGTACCAAGCATGCCATTCTCAAGGGCTCGAGCACTGCCCACTGCCTTTTTGACTGACATGCTAAGGCCTATTGCCACCGGTTTGACTCCCTACATGGGAAGGCTATGTGTATCCCAGAGTCTGTATTCATGGCCCTTTTTTGTTGATGTCGGGGGAGAAATCTATGATCCCGAAACAAATTCTTGGGATGAAATGCCTCTTGGCATGGGAGATGGTTGGCCTGCTCGGCAGGCTGGTACAAAGTTGAGTGTTGTGGTAGATGGTGAATTGTATGCTTTTGATCCTTCGAGTTCTTTGGATAGTGGTAAGATCAAGGTGTACGATCAAGGAGAAGATGCTTGGAAAGTTGTTATAGGAAAAGTCCCTATTCATGACTTTGCTGGTTCAGATTCTCCATATTTACTTGCTGGTTTTCATGGAAAACTTCATGTCATCACAAAAGATGCAAATCATGAAATCGCAGTTCTGCGTGCTGACCTGTGCAGTAATTTGGGTTCTTTGTCACCAACCTTAGCTCCTCTCCTAGCTAGCTCCTCAGATGAACATTCCGACGCACTGGCAGAATCAGACGCAGTTGTGTGGAAGATCATTGGGACTCGGGATTTTGGTTCAGGTGAATTGGTTGGCTGTCAAGTTCTTGATCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_1

Protein Analysis

448

Amino Acids

49.68

Weight (kDa)

5.54

Isoelectric Point (pI)

49.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 13 - 54 9.1e-10 F-box domain
F-box-like PF12937 13 - 53 1e-05 F-box-like
Kelch_FKB95 PF25210 139 - 380 1.4e-17 FKB95, Kelch-repeats domain
Kelch_1 PF01344 141 - 186 3.3e-12 Kelch motif
Kelch_KLHDC2_KLHL20_DRC7 PF24681 146 - 236 7.5e-08 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_Calicin PF13964 147 - 239 2.1e-07 Calicin, beta-propeller domain
Kelch_1 PF01344 189 - 237 3.9e-11 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 483
AasI GACNNNNNNGTC 1 cut(s) 553
AclWI GGATC 6 cut(s) 58, 242, 512, 637, 857, 971
AcsI RAATTY 2 cut(s) 90, 874
AcuI CTGAAG 3 cut(s) 635, 681, 714
AfaI GTAC 6 cut(s) 13, 200, 685, 699, 934, 1016
AfiI CCNNNNNNNGG 4 cut(s) 126, 428, 680, 791
AgeI ACCGGT 1 cut(s) 773
AgsI TTSAA 3 cut(s) 103, 218, 413
AjnI CCWGG 1 cut(s) 233
AjuI GAANNNNNNNTTGG 4 cut(s) 21, 53, 879, 911
AluBI AGCT 6 cut(s) 58, 147, 488, 1211, 1223, 1227
AluI AGCT 6 cut(s) 58, 147, 488, 1211, 1223, 1227
Alw21I GWGCWC 1 cut(s) 730
AlwI GGATC 6 cut(s) 58, 242, 512, 637, 857, 971
AlwNI CAGNNNCTG 1 cut(s) 817
Ama87I CYCGRG 3 cut(s) 624, 723, 1293
AoxI GGCC 4 cut(s) 352, 761, 827, 914
ApaI GGGCCC 1 cut(s) 356
ApoI RAATTY 2 cut(s) 90, 874
AsiGI ACCGGT 1 cut(s) 773
AspS9I GGNCC 3 cut(s) 352, 353, 828
AsuC2I CCSGG 1 cut(s) 121
AsuHPI GGTGA 3 cut(s) 971, 1191, 1322
AsuNHI GCTAGC 1 cut(s) 1223
AvaI CYCGRG 3 cut(s) 624, 723, 1293
BaeGI GKGCMC 1 cut(s) 356
BanII GRGCYC 3 cut(s) 356, 675, 724
Bbv12I GWGCWC 1 cut(s) 730
BccI CCATC 3 cut(s) 446, 902, 950
BceAI ACGGC 1 cut(s) 406
BciT130I CCWGG 1 cut(s) 235
BciVI GTATCC 1 cut(s) 817
BcnI CCSGG 1 cut(s) 121
BfaI CTAG 6 cut(s) 87, 144, 327, 470, 1220, 1224
BfuI GTATCC 1 cut(s) 817
BglI GCCNNNNNGGC 1 cut(s) 922
Bme1390I CCNGG 2 cut(s) 121, 235
BmeT110I CYCGRG 3 cut(s) 624, 723, 1293
BmgT120I GGNCC 3 cut(s) 352, 353, 828
BmiI GGNNCC 1 cut(s) 354
BmrFI CCNGG 2 cut(s) 121, 235
BmsI GCATC 4 cut(s) 273, 446, 1021, 1129
BmtI GCTAGC 1 cut(s) 1227
BplI GAGNNNNNCTC 2 cut(s) 262, 294
BpmI CTGGAG 1 cut(s) 529
Bpu10I CCTNAGC 2 cut(s) 758, 1207
BpuEI CTTGAG 1 cut(s) 700
BpuMI CCSGG 1 cut(s) 121
Bsa29I ATCGAT 1 cut(s) 62
BsaBI GATNNNNATC 1 cut(s) 1143
BsaWI WCCGGW 1 cut(s) 773
BsaXI ACNNNNNCTCC 2 cut(s) 1195, 1225
Bsc4I CCNNNNNNNGG 4 cut(s) 126, 428, 680, 791
Bse118I RCCGGY 1 cut(s) 773
Bse1I ACTGG 2 cut(s) 685, 1257
Bse8I GATNNNNATC 1 cut(s) 1143
BseBI CCWGG 1 cut(s) 235
BseCI ATCGAT 1 cut(s) 62
BseGI GGATG 2 cut(s) 461, 889
BseJI GATNNNNATC 1 cut(s) 1143
BseLI CCNNNNNNNGG 4 cut(s) 126, 428, 680, 791
BseMII CTCAG 2 cut(s) 293, 1245
BseNI ACTGG 2 cut(s) 685, 1257
BseRI GAGGAG 3 cut(s) 35, 1203, 1219
BseSI GKGCMC 1 cut(s) 356
BsgI GTGCAG 1 cut(s) 1198
Bsh1285I CGRYCG 1 cut(s) 495
BshFI GGCC 4 cut(s) 354, 763, 829, 916
BshTI ACCGGT 1 cut(s) 773
BshVI ATCGAT 1 cut(s) 62
BsiEI CGRYCG 1 cut(s) 495
BsiHKAI GWGCWC 1 cut(s) 730
BsiHKCI CYCGRG 3 cut(s) 624, 723, 1293
BsiSI CCGG 2 cut(s) 121, 774
BslFI GGGAC 2 cut(s) 1043, 1303
BslI CCNNNNNNNGG 4 cut(s) 126, 428, 680, 791
BsmFI GGGAC 2 cut(s) 1043, 1303
BsmI GAATGC 1 cut(s) 535
BsnI GGCC 4 cut(s) 354, 763, 829, 916
BsoBI CYCGRG 3 cut(s) 624, 723, 1293
Bsp120I GGGCCC 1 cut(s) 352
Bsp1286I GDGCHC 4 cut(s) 356, 675, 724, 730
BspANI GGCC 4 cut(s) 354, 763, 829, 916
BspCNI CTCAG 2 cut(s) 292, 1244
BspDI ATCGAT 1 cut(s) 62
BspHI TCATGA 2 cut(s) 1066, 1147
BspLI GGNNCC 1 cut(s) 354
BspOI GCTAGC 1 cut(s) 1227
BspPI GGATC 6 cut(s) 58, 242, 512, 637, 857, 971
BsrFI RCCGGY 1 cut(s) 773
BsrI ACTGG 2 cut(s) 685, 1257
BssAI RCCGGY 1 cut(s) 773
Bst2UI CCWGG 1 cut(s) 235
Bst4CI ACNGT 2 cut(s) 139, 496
Bst6I CTCTTC 3 cut(s) 213, 300, 654
BstC8I GCNNGC 6 cut(s) 240, 707, 918, 927, 1167, 1225
BstDEI CTNAG 4 cut(s) 279, 758, 1207, 1231
BstF5I GGATG 2 cut(s) 461, 889
BstMCI CGRYCG 1 cut(s) 495
BstMWI GCNNNNNNNGC 5 cut(s) 443, 557, 566, 922, 926
BstNI CCWGG 1 cut(s) 235
BstNSI RCATGY 3 cut(s) 242, 709, 757
BstSCI CCNGG 2 cut(s) 119, 233
BstSLI GKGCMC 1 cut(s) 356
Bsu15I ATCGAT 1 cut(s) 62
BsuI GTATCC 1 cut(s) 817
BsuRI GGCC 4 cut(s) 354, 763, 829, 916
BsuTUI ATCGAT 1 cut(s) 62
BtsCI GGATG 2 cut(s) 461, 889
BtsI GCAGTG 2 cut(s) 728, 735
BtsIMutI CAGTG 4 cut(s) 678, 728, 735, 1250
Cac8I GCNNGC 6 cut(s) 240, 707, 918, 927, 1167, 1225
CaiI CAGNNNCTG 1 cut(s) 817
CciI TCATGA 2 cut(s) 1066, 1147
Cfr10I RCCGGY 1 cut(s) 773
Cfr13I GGNCC 3 cut(s) 352, 353, 828
ClaI ATCGAT 1 cut(s) 62
CseI GACGC 3 cut(s) 493, 1256, 1274
Csp6I GTAC 6 cut(s) 12, 199, 684, 698, 933, 1015
CspAI ACCGGT 1 cut(s) 773
CviQI GTAC 6 cut(s) 12, 199, 684, 698, 933, 1015
DdeI CTNAG 4 cut(s) 279, 758, 1207, 1231
DraI TTTAAA 1 cut(s) 160
DrdI GACNNNNNNGTC 1 cut(s) 553
DseDI GACNNNNNNGTC 1 cut(s) 553
Eam1104I CTCTTC 3 cut(s) 213, 300, 654
EarI CTCTTC 3 cut(s) 213, 300, 654
Eco147I AGGCCT 1 cut(s) 763
Eco24I GRGCYC 3 cut(s) 356, 675, 724
Eco57I CTGAAG 3 cut(s) 635, 681, 714
Eco88I CYCGRG 3 cut(s) 624, 723, 1293
EcoRII CCWGG 1 cut(s) 233
EcoT22I ATGCAT 1 cut(s) 535
EcoT38I GRGCYC 3 cut(s) 356, 675, 724
FaqI GGGAC 2 cut(s) 1043, 1303
FokI GGATG 2 cut(s) 468, 896
FriOI GRGCYC 3 cut(s) 356, 675, 724
FspBI CTAG 6 cut(s) 87, 144, 327, 470, 1220, 1224
GsuI CTGGAG 1 cut(s) 529
HaeIII GGCC 4 cut(s) 354, 763, 829, 916
HapII CCGG 2 cut(s) 121, 774
HgaI GACGC 3 cut(s) 493, 1256, 1274
HincII GTYRAC 1 cut(s) 207
HindII GTYRAC 1 cut(s) 207
HinfI GANTC 6 cut(s) 81, 781, 814, 1085, 1259, 1291
HpaII CCGG 2 cut(s) 121, 774
HphI GGTGA 3 cut(s) 971, 1191, 1322
Hpy166II GTNNAC 2 cut(s) 207, 1015
Hpy188I TCNGA 9 cut(s) 151, 282, 528, 591, 694, 1084, 1234, 1246, 1264
Hpy188III TCNNGA 9 cut(s) 50, 258, 508, 626, 866, 1067, 1148, 1295, 1334
Hpy8I GTNNAC 2 cut(s) 207, 1015
Hpy99I CGWCG 1 cut(s) 1250
HpyAV CCTTC 4 cut(s) 124, 255, 789, 990
HpyCH4III ACNGT 2 cut(s) 139, 496
HpyCH4V TGCA 4 cut(s) 533, 560, 1142, 1179
HpyF10VI GCNNNNNNNGC 5 cut(s) 443, 557, 566, 922, 926
HpyF3I CTNAG 4 cut(s) 279, 758, 1207, 1231
LmnI GCTCC 2 cut(s) 1216, 1232
LweI GCATC 4 cut(s) 273, 446, 1021, 1129
MaeI CTAG 6 cut(s) 87, 144, 327, 470, 1220, 1224
MaeIII GTNAC 5 cut(s) 3, 139, 544, 619, 1197
MboII GAAGA 9 cut(s) 38, 200, 230, 314, 315, 317, 641, 1040, 1291
MhlI GDGCHC 4 cut(s) 356, 675, 724, 730
MlyI GAGTC 4 cut(s) 75, 775, 823, 1285
MmeI TCCRAC 2 cut(s) 592, 1269
Mph1103I ATGCAT 1 cut(s) 535
MseI TTAA 1 cut(s) 159
MspI CCGG 2 cut(s) 121, 774
MspR9I CCNGG 2 cut(s) 121, 235
Mva1269I GAATGC 1 cut(s) 535
MvaI CCWGG 1 cut(s) 235
MwoI GCNNNNNNNGC 5 cut(s) 443, 557, 566, 922, 926
NciI CCSGG 1 cut(s) 121
NheI GCTAGC 1 cut(s) 1223
NlaIV GGNNCC 1 cut(s) 354
NmeAIII GCCGAG 1 cut(s) 901
NmuCI GTSAC 3 cut(s) 3, 544, 1197
NsiI ATGCAT 1 cut(s) 535
NspI RCATGY 3 cut(s) 242, 709, 757
PaeI GCATGC 2 cut(s) 242, 709
PaeR7I CTCGAG 1 cut(s) 723
PagI TCATGA 2 cut(s) 1066, 1147
PceI AGGCCT 1 cut(s) 763
PctI GAATGC 1 cut(s) 535
PfeI GAWTC 2 cut(s) 1085, 1259
PfoI TCCNGGA 1 cut(s) 119
PinAI ACCGGT 1 cut(s) 773
PleI GAGTC 4 cut(s) 75, 775, 822, 1285
PpsI GAGTC 4 cut(s) 75, 775, 822, 1285
PsiI TTATAA 1 cut(s) 483
Psp6I CCWGG 1 cut(s) 233
PspGI CCWGG 1 cut(s) 233
PspN4I GGNNCC 1 cut(s) 354
PspOMI GGGCCC 1 cut(s) 352
PspPI GGNCC 3 cut(s) 352, 353, 828
PspXI VCTCGAGB 1 cut(s) 723
PsrI GAACNNNNNNTAC 2 cut(s) 1174, 1206
PstNI CAGNNNCTG 1 cut(s) 817
RsaI GTAC 6 cut(s) 13, 200, 685, 699, 934, 1016
RsaNI GTAC 6 cut(s) 12, 199, 684, 698, 933, 1015
SaqAI TTAA 1 cut(s) 159
Sau96I GGNCC 3 cut(s) 352, 353, 828
SchI GAGTC 4 cut(s) 75, 775, 823, 1285
ScrFI CCNGG 2 cut(s) 121, 235
SduI GDGCHC 4 cut(s) 356, 675, 724, 730
SfaNI GCATC 4 cut(s) 273, 446, 1021, 1129
Sfr274I CTCGAG 1 cut(s) 723
SlaI CTCGAG 1 cut(s) 723
SmiI ATTTAAAT 1 cut(s) 160
SmlI CTYRAG 2 cut(s) 715, 723
SmoI CTYRAG 2 cut(s) 715, 723
SphI GCATGC 2 cut(s) 242, 709
SseBI AGGCCT 1 cut(s) 763
SspMI CTAG 6 cut(s) 87, 144, 327, 470, 1220, 1224
StuI AGGCCT 1 cut(s) 763
StyD4I CCNGG 2 cut(s) 119, 233
SwaI ATTTAAAT 1 cut(s) 160
TaaI ACNGT 2 cut(s) 139, 496
TaqI TCGA 6 cut(s) 62, 246, 257, 335, 724, 983
TatI WGTACW 2 cut(s) 11, 198
TfiI GAWTC 2 cut(s) 1085, 1259
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TscAI CASTG 4 cut(s) 685, 735, 742, 1257
TseFI GTSAC 3 cut(s) 3, 544, 1197
Tsp45I GTSAC 3 cut(s) 3, 544, 1197
TspDTI ATGAA 8 cut(s) 315, 813, 900, 1055, 1100, 1112, 1164, 1251
TspRI CASTG 4 cut(s) 685, 735, 742, 1257
XapI RAATTY 2 cut(s) 90, 874
XceI RCATGY 3 cut(s) 242, 709, 757
XhoI CTCGAG 1 cut(s) 723
XspI CTAG 6 cut(s) 87, 144, 327, 470, 1220, 1224
Zsp2I ATGCAT 1 cut(s) 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.