pycom15g32480

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0006242
Physical Location & Seq
Reverse (-)
31499238 .. 31500320
1083 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g32480.1

Sequence Viewer

Length: 1083 bp
ATGTGGCAGAGGCTACCTCAGATGCCCAATGCAGTTTATGAAGAAGAATACAGTAAGGGTTCTTCTAGGTTTTCGATGTGGAATATGGTGGGCCCAAGTCTCAAAATTGCTCATACTATTAGAGGCTGGCTAGGGAGAAAGAACTCGTTTGAACAAATGCCATTTTGCGGTTGTGCCATTGGTGCTGTTGATGGATGCCTTTACGTTCTAGGTGGATTTTATAAAACTACGACCATGAGGTGTGTCTGGAGATTTGATCCAATTCAGAATGCATGGAGTGAAGTGACAGCCATGTCTGCTAGTAGGGCCTATTGTAAGACAAGCATTCTGAATAATAAATTATATGTTGTTGGAGGAGTTAGTCGGGAACGAGGTGGATTGATCCCCCTTCAGTCTGCCGAAGTTTTTGAGCCCTCCACTGGTACGTGGTCCGAAGTACCAAGCATGCCATTCTCAAGGGCTCAAGCCTTGCCCACTGCCTTTTTGACTGACATGCTAAGGCCTATTGCCACTGGGTTGACTTCCTACATGGGAAGACTATGCGTATCCCAGAGTCTGTATTCATGGCCCTTTTTTGTTGATGTTGGGGGAGAAATCTATGATCCCGAGACAAATTCTTGGGATGAAATGCCTCTTGGCATGGGAGAGGATTGGCCTGCTCGGCAGGCAGGTACAAAATTAAGCATTGTGGTAGATGGTGAATTGTATGCTTTTGATCCTTCGAGTTCATTGGATAGTGGTAAGATCAAGGTGTACGATCAAGGAGAAGATGCTTGGAAAGTTGTTATAGGAAAAGTCCCTATTCATGACTTTGCTGGTTCAGATTCTCCATATTTACTTGCTGGTTTTCATGGAAAACTTCATGTCATCACAAAAGATGCGAATCATGAAATCGCAGTTCTGCGTGCTGACCTGTGCAGTAATTTGGGTTCTTTCTCATCAAGATCAACTTCTCTCATGCCTAGCTCCTCGCATGAACATTCCAACACTGCGGCAGAATCAGACACAGTTATGTGGAAGGTCATTGGGACGAGGGATTTTGGTTCAGGTGAATTGGTTGGTTGTCAAATTCTTGATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_1

Protein Analysis

361

Amino Acids

39.6

Weight (kDa)

5.43

Isoelectric Point (pI)

43.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_FKB95 PF25210 48 - 293 4.9e-19 FKB95, Kelch-repeats domain
Kelch_1 PF01344 54 - 99 1.8e-12 Kelch motif
Kelch_KLHDC2_KLHL20_DRC7 PF24681 59 - 149 1.6e-08 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_Calicin PF13964 60 - 152 8.5e-08 Calicin, beta-propeller domain
Beta-prop_FBX42 PF13415 64 - 146 3.2e-06 FBX42, beta-propeller domain
Kelch_1 PF01344 102 - 150 4.6e-11 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 222
AasI GACNNNNNNGTC 1 cut(s) 292
Acc36I ACCTGC 1 cut(s) 659
AciI CCGC 2 cut(s) 168, 992
AclWI GGATC 4 cut(s) 251, 376, 596, 710
AcsI RAATTY 2 cut(s) 613, 1068
AcuI CTGAAG 1 cut(s) 374
AfaI GTAC 4 cut(s) 424, 438, 673, 755
AfiI CCNNNNNNNGG 2 cut(s) 167, 419
AgsI TTSAA 1 cut(s) 152
AjuI GAANNNNNNNTTGG 2 cut(s) 618, 650
AluBI AGCT 1 cut(s) 966
AluI AGCT 1 cut(s) 966
Alw26I GTCTC 2 cut(s) 104, 602
AlwI GGATC 4 cut(s) 251, 376, 596, 710
AlwNI CAGNNNCTG 1 cut(s) 556
Ama87I CYCGRG 1 cut(s) 605
AoxI GGCC 5 cut(s) 91, 306, 500, 566, 653
ApaI GGGCCC 1 cut(s) 95
ApoI RAATTY 2 cut(s) 613, 1068
AspS9I GGNCC 5 cut(s) 91, 92, 306, 429, 567
AsuHPI GGTGA 2 cut(s) 710, 1061
AvaI CYCGRG 1 cut(s) 605
AvaII GGWCC 1 cut(s) 429
BaeGI GKGCMC 1 cut(s) 95
BanII GRGCYC 3 cut(s) 95, 414, 463
BarI GAAGNNNNNNTAC 2 cut(s) 46, 78
BbsI GAAGAC 1 cut(s) 541
BccI CCATC 2 cut(s) 185, 689
BciVI GTATCC 1 cut(s) 556
BcoDI GTCTC 2 cut(s) 104, 602
BfaI CTAG 5 cut(s) 66, 131, 209, 300, 963
BfuAI ACCTGC 1 cut(s) 659
BfuI GTATCC 1 cut(s) 556
BglI GCCNNNNNGGC 1 cut(s) 661
BisI GCNGC 1 cut(s) 993
BlsI GCNGC 1 cut(s) 994
Bme18I GGWCC 1 cut(s) 429
BmeT110I CYCGRG 1 cut(s) 605
BmgT120I GGNCC 5 cut(s) 91, 92, 306, 429, 567
BmiI GGNNCC 1 cut(s) 93
BmrI ACTGGG 1 cut(s) 522
BmsI GCATC 4 cut(s) 12, 185, 760, 868
BmuI ACTGGG 1 cut(s) 522
BpiI GAAGAC 1 cut(s) 541
BplI GAGNNNNNCTC 1 cut(s) 33
BpmI CTGGAG 1 cut(s) 268
Bpu10I CCTNAGC 1 cut(s) 497
BpuEI CTTGAG 2 cut(s) 439, 447
BsaAI YACGTR 1 cut(s) 426
BsaBI GATNNNNATC 1 cut(s) 882
Bsc4I CCNNNNNNNGG 2 cut(s) 167, 419
Bse1I ACTGG 2 cut(s) 424, 517
Bse8I GATNNNNATC 1 cut(s) 882
BseGI GGATG 2 cut(s) 200, 628
BseJI GATNNNNATC 1 cut(s) 882
BseLI CCNNNNNNNGG 2 cut(s) 167, 419
BseMII CTCAG 1 cut(s) 32
BseNI ACTGG 2 cut(s) 424, 517
BseRI GAGGAG 2 cut(s) 369, 958
BseSI GKGCMC 1 cut(s) 95
BsgI GTGCAG 1 cut(s) 937
BshFI GGCC 5 cut(s) 93, 308, 502, 568, 655
BsiHKCI CYCGRG 1 cut(s) 605
BslFI GGGAC 2 cut(s) 782, 1042
BslI CCNNNNNNNGG 2 cut(s) 167, 419
BsmAI GTCTC 2 cut(s) 104, 602
BsmFI GGGAC 2 cut(s) 782, 1042
BsmI GAATGC 2 cut(s) 274, 324
BsnI GGCC 5 cut(s) 93, 308, 502, 568, 655
BsoBI CYCGRG 1 cut(s) 605
Bsp120I GGGCCC 1 cut(s) 91
Bsp1286I GDGCHC 3 cut(s) 95, 414, 463
Bsp143I GATC 7 cut(s) 256, 381, 601, 715, 744, 757, 944
BspACI CCGC 2 cut(s) 168, 992
BspANI GGCC 5 cut(s) 93, 308, 502, 568, 655
BspCNI CTCAG 1 cut(s) 31
BspHI TCATGA 2 cut(s) 805, 886
BspLI GGNNCC 1 cut(s) 93
BspMI ACCTGC 1 cut(s) 659
BspPI GGATC 4 cut(s) 251, 376, 596, 710
BsrI ACTGG 2 cut(s) 424, 517
BssMI GATC 7 cut(s) 256, 381, 601, 715, 744, 757, 944
Bst4CI ACNGT 2 cut(s) 53, 1009
BstBAI YACGTR 1 cut(s) 426
BstC8I GCNNGC 5 cut(s) 128, 446, 657, 666, 906
BstDEI CTNAG 2 cut(s) 18, 497
BstF5I GGATG 2 cut(s) 200, 628
BstKTI GATC 7 cut(s) 259, 384, 604, 718, 747, 760, 947
BstMAI GTCTC 2 cut(s) 104, 602
BstMBI GATC 7 cut(s) 256, 381, 601, 715, 744, 757, 944
BstMWI GCNNNNNNNGC 5 cut(s) 182, 296, 305, 661, 665
BstNSI RCATGY 2 cut(s) 448, 496
BstSLI GKGCMC 1 cut(s) 95
BstV2I GAAGAC 1 cut(s) 541
BsuI GTATCC 1 cut(s) 556
BsuRI GGCC 5 cut(s) 93, 308, 502, 568, 655
BtsCI GGATG 2 cut(s) 200, 628
BtsI GCAGTG 2 cut(s) 474, 987
BtsIMutI CAGTG 4 cut(s) 417, 474, 510, 987
BveI ACCTGC 1 cut(s) 659
Cac8I GCNNGC 5 cut(s) 128, 446, 657, 666, 906
CaiI CAGNNNCTG 1 cut(s) 556
CciI TCATGA 2 cut(s) 805, 886
Cfr13I GGNCC 5 cut(s) 91, 92, 306, 429, 567
Csp6I GTAC 4 cut(s) 423, 437, 672, 754
CviQI GTAC 4 cut(s) 423, 437, 672, 754
DdeI CTNAG 2 cut(s) 18, 497
DpnI GATC 7 cut(s) 258, 383, 603, 717, 746, 759, 946
DpnII GATC 7 cut(s) 256, 381, 601, 715, 744, 757, 944
DrdI GACNNNNNNGTC 1 cut(s) 292
DseDI GACNNNNNNGTC 1 cut(s) 292
Eco147I AGGCCT 1 cut(s) 502
Eco24I GRGCYC 3 cut(s) 95, 414, 463
Eco47I GGWCC 1 cut(s) 429
Eco57I CTGAAG 1 cut(s) 374
Eco88I CYCGRG 1 cut(s) 605
EcoO109I RGGNCCY 1 cut(s) 306
EcoT22I ATGCAT 1 cut(s) 274
EcoT38I GRGCYC 3 cut(s) 95, 414, 463
FalI AAGNNNNNCTT 2 cut(s) 934, 966
FaqI GGGAC 2 cut(s) 782, 1042
Fnu4HI GCNGC 1 cut(s) 993
FokI GGATG 2 cut(s) 207, 635
FriOI GRGCYC 3 cut(s) 95, 414, 463
Fsp4HI GCNGC 1 cut(s) 993
FspBI CTAG 5 cut(s) 66, 131, 209, 300, 963
GluI GCNGC 1 cut(s) 993
GsuI CTGGAG 1 cut(s) 268
HaeIII GGCC 5 cut(s) 93, 308, 502, 568, 655
HincII GTYRAC 1 cut(s) 519
HindII GTYRAC 1 cut(s) 519
HinfI GANTC 4 cut(s) 553, 824, 883, 998
HphI GGTGA 2 cut(s) 710, 1061
Hpy166II GTNNAC 2 cut(s) 519, 754
Hpy188I TCNGA 6 cut(s) 21, 267, 330, 433, 823, 1003
Hpy188III TCNNGA 7 cut(s) 247, 365, 605, 806, 887, 942, 1073
Hpy8I GTNNAC 2 cut(s) 519, 754
HpyAV CCTTC 3 cut(s) 398, 729, 1012
HpyCH4III ACNGT 2 cut(s) 53, 1009
HpyCH4IV ACGT 2 cut(s) 204, 425
HpyCH4V TGCA 3 cut(s) 32, 272, 918
HpyF10VI GCNNNNNNNGC 5 cut(s) 182, 296, 305, 661, 665
HpyF3I CTNAG 2 cut(s) 18, 497
HpySE526I ACGT 2 cut(s) 204, 425
Kzo9I GATC 7 cut(s) 256, 381, 601, 715, 744, 757, 944
LmnI GCTCC 1 cut(s) 971
LweI GCATC 4 cut(s) 12, 185, 760, 868
MaeI CTAG 5 cut(s) 66, 131, 209, 300, 963
MaeII ACGT 2 cut(s) 204, 425
MaeIII GTNAC 1 cut(s) 283
MalI GATC 7 cut(s) 258, 383, 603, 717, 746, 759, 946
MboI GATC 7 cut(s) 256, 381, 601, 715, 744, 757, 944
MboII GAAGA 5 cut(s) 53, 54, 56, 546, 779
MhlI GDGCHC 3 cut(s) 95, 414, 463
MluCI AATT 9 cut(s) 105, 261, 338, 613, 677, 701, 922, 1052, 1068
MlyI GAGTC 1 cut(s) 562
MmeI TCCRAC 2 cut(s) 331, 1008
Mph1103I ATGCAT 1 cut(s) 274
MseI TTAA 1 cut(s) 680
MslI CAYNNNNRTG 1 cut(s) 1010
Mva1269I GAATGC 2 cut(s) 274, 324
MwoI GCNNNNNNNGC 5 cut(s) 182, 296, 305, 661, 665
NdeII GATC 7 cut(s) 256, 381, 601, 715, 744, 757, 944
NlaIV GGNNCC 1 cut(s) 93
NmeAIII GCCGAG 1 cut(s) 640
NmuCI GTSAC 1 cut(s) 283
NsiI ATGCAT 1 cut(s) 274
NspI RCATGY 2 cut(s) 448, 496
PaeI GCATGC 1 cut(s) 448
PagI TCATGA 2 cut(s) 805, 886
PceI AGGCCT 1 cut(s) 502
PctI GAATGC 2 cut(s) 274, 324
PfeI GAWTC 3 cut(s) 824, 883, 998
PkrI GCNGC 1 cut(s) 994
PleI GAGTC 1 cut(s) 561
PpsI GAGTC 1 cut(s) 561
Ppu21I YACGTR 1 cut(s) 426
PsiI TTATAA 1 cut(s) 222
PspN4I GGNNCC 1 cut(s) 93
PspOMI GGGCCC 1 cut(s) 91
PspPI GGNCC 5 cut(s) 91, 92, 306, 429, 567
PsrI GAACNNNNNNTAC 2 cut(s) 913, 945
PstNI CAGNNNCTG 1 cut(s) 556
RsaI GTAC 4 cut(s) 424, 438, 673, 755
RsaNI GTAC 4 cut(s) 423, 437, 672, 754
RseI CAYNNNNRTG 1 cut(s) 1010
SaqAI TTAA 1 cut(s) 680
SatI GCNGC 1 cut(s) 993
Sau3AI GATC 7 cut(s) 256, 381, 601, 715, 744, 757, 944
Sau96I GGNCC 5 cut(s) 91, 92, 306, 429, 567
SchI GAGTC 1 cut(s) 562
SduI GDGCHC 3 cut(s) 95, 414, 463
SfaNI GCATC 4 cut(s) 12, 185, 760, 868
SinI GGWCC 1 cut(s) 429
SmiMI CAYNNNNRTG 1 cut(s) 1010
SmlI CTYRAG 2 cut(s) 454, 462
SmoI CTYRAG 2 cut(s) 454, 462
SphI GCATGC 1 cut(s) 448
Sse9I AATT 9 cut(s) 105, 261, 338, 613, 677, 701, 922, 1052, 1068
SseBI AGGCCT 1 cut(s) 502
SsiI CCGC 2 cut(s) 168, 992
SspMI CTAG 5 cut(s) 66, 131, 209, 300, 963
StuI AGGCCT 1 cut(s) 502
TaaI ACNGT 2 cut(s) 53, 1009
TaiI ACGT 2 cut(s) 207, 428
TaqI TCGA 2 cut(s) 74, 722
TasI AATT 9 cut(s) 105, 261, 338, 613, 677, 701, 922, 1052, 1068
TauI GCSGC 1 cut(s) 995
TfiI GAWTC 3 cut(s) 824, 883, 998
Tru1I TTAA 1 cut(s) 680
Tru9I TTAA 1 cut(s) 680
TscAI CASTG 4 cut(s) 424, 481, 517, 994
TseFI GTSAC 1 cut(s) 283
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 9 cut(s) 54, 552, 639, 717, 794, 839, 851, 903, 990
TspRI CASTG 4 cut(s) 424, 481, 517, 994
VpaK11BI GGWCC 1 cut(s) 429
XapI RAATTY 2 cut(s) 613, 1068
XceI RCATGY 2 cut(s) 448, 496
XspI CTAG 5 cut(s) 66, 131, 209, 300, 963
Zsp2I ATGCAT 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.