MD08G1180200.v1.1

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0013977
Physical Location & Seq
Forward (+)
22181005 .. 22182069
1065 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1180200.v1.1.491

Sequence Viewer

Length: 1065 bp
ATGGCAGCTACAATGGATTTCTACAGTGGCAGATCAGTTCTTCACTCATCATCAGATCCCTTTAGAGGTGAGCTGATGGAAGCACTTGAGCCTTTTATGAAAAGTGTTTCTTCATCGCCGCCGTCTTCCCTTCCCACCACCACCGCTTCTCCTTCCTTTTCCTCCGCCCCTCTCCTCCCTTCTTTCCCCTTTACCTCCTACGACTTCCTCTCTTTCCCAAACCAGCCCAATTTCTACCACGACGGTTGCTCCACCTCTTCTATGACCCAAGTGTTTTCAGATGGGTTCTTTGAGCAGCAACCGCCGTCAATCGGGCTGAACCACCTCACCTCCTCCCAGATCCACCAGATCCAAGCTCAGATCCAGTCTCAATCCCAACCCCAACAGCCCCAAATCTCTTGGGATAACTACCACCAACAAGTTTCTCAGACGACGTCTCGTACCCTCAGCTTCCTCAGCCCCAAGCCCATCCCGATGAAGCAGGTGGGCTCGGCTGGTTCGCCGCCGAAACCCACCAAGCTTTACAGGGGTGTAAGGCAACGCCACTGGGGAAAATGGGTCGCTGAGATCAGGCTCCCTAAGAACCGAACCAGGCTCTGGCTCGGAACTTTCGACACGGCGGAGGAAGCCGCTTTGGCTTACGACAAGGCGGCGTACAAGCTCAGAGGCGACTTCGCCAGGCTCAATTTCCCGCACCTCCGCCACAACGGGTCCGTCGTTGGAGGCGAGTTCGGCGAGTACAAGCCTCTGCATTCCTCAGTCGACGCCAAGCTTCAAGCCATCTGCGAAGGCTTGGCCGAAACTCAGAAACAGGGCAAGTCGGGGAAGCCAGCCAAGAGGTCCTCCGCCGCCAGCCGACAGCCGGAGCCGGTGACTTTAGCGGTCAAGGTTGAAACCTCGTCGCCGGTGACGACTGATAGCGAGGGGTCTGCCGGGTCTTCGCCGCTGTCTGATCTGACTTTCTGCGACTCCATGGAGCCATGGGAGAACAGCGGCGCTTCCGAGAGCAGTTCCGTGTTGGAGAAGTATCCGTCCGAGATTGATTGGGCTTCGATCCTCAGTTAA

Protein Analysis

355

Amino Acids

38.75

Weight (kDa)

6.51

Isoelectric Point (pI)

61.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 174 - 223 5e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013977)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78080
fragaria_vesca FvH4_5g37820
malus_domestica MD08G1180200.v1.1 MD15G1365500.v1.1
prunus_persica Prupe.1G513600_v2.0.a1 Prupe.3G009400_v2.0.a1
pyrus_communis pycom08g15420 pycom15g32790
rosa_chinensis RchiOBHm_Chr7g0239701
rosa_laevigata RLG00000000738
rosa_multiflora Rmu_sc0000063.1_g000026
rosa_roxburghii Rroxscaffold_3G00220810
rosa_samantha Rh7AG483000 Rh7BG455900 Rh7CG500600 Rh7DG468800
rosa_wichuraiana Rw7G040430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 472
AatII GACGTC 1 cut(s) 437
Acc36I ACCTGC 1 cut(s) 472
AccB7I CCANNNNNTGG 1 cut(s) 597
AccI GTMKAC 1 cut(s) 762
AclWI GGATC 5 cut(s) 50, 334, 343, 355, 1048
AcoI YGGCCR 1 cut(s) 795
AcyI GRCGYC 2 cut(s) 434, 765
AfaI GTAC 3 cut(s) 442, 656, 740
AfiI CCNNNNNNNGG 4 cut(s) 65, 311, 597, 862
AgsI TTSAA 2 cut(s) 776, 893
AjnI CCWGG 2 cut(s) 590, 677
AluBI AGCT 7 cut(s) 8, 73, 356, 450, 520, 661, 772
AluI AGCT 7 cut(s) 8, 73, 356, 450, 520, 661, 772
Alw26I GTCTC 2 cut(s) 372, 441
AlwI GGATC 5 cut(s) 50, 334, 343, 355, 1048
AlwNI CAGNNNCTG 1 cut(s) 597
AoxI GGCC 1 cut(s) 795
ApeKI GCWGC 2 cut(s) 5, 295
AspLEI GCGC 1 cut(s) 998
AspS9I GGNCC 2 cut(s) 711, 840
AsuC2I CCSGG 1 cut(s) 934
AsuHPI GGTGA 4 cut(s) 80, 319, 883, 919
AvaII GGWCC 2 cut(s) 711, 840
BanII GRGCYC 1 cut(s) 491
BbsI GAAGAC 2 cut(s) 117, 930
BbvCI CCTCAGC 2 cut(s) 446, 455
BbvI GCAGC 2 cut(s) 17, 307
BccI CCATC 4 cut(s) 70, 275, 476, 788
BceAI ACGGC 3 cut(s) 106, 289, 633
BcgI CGANNNNNNTGC 2 cut(s) 911, 945
BciT130I CCWGG 2 cut(s) 592, 679
BciVI GTATCC 1 cut(s) 1038
BcnI CCSGG 1 cut(s) 934
BcoDI GTCTC 2 cut(s) 372, 441
BfmI CTRYAG 1 cut(s) 22
BfoI RGCGCY 1 cut(s) 999
BfuAI ACCTGC 1 cut(s) 472
BfuI GTATCC 1 cut(s) 1038
BglI GCCNNNNNGGC 1 cut(s) 635
BisI GCNGC 9 cut(s) 6, 119, 296, 503, 630, 651, 849, 944, 994
BlsI GCNGC 9 cut(s) 7, 120, 297, 504, 631, 652, 850, 945, 995
Bme1390I CCNGG 3 cut(s) 592, 679, 934
Bme18I GGWCC 2 cut(s) 711, 840
BmgT120I GGNCC 2 cut(s) 711, 840
BmiI GGNNCC 4 cut(s) 575, 712, 867, 978
BmrFI CCNGG 3 cut(s) 592, 679, 934
BmrI ACTGGG 1 cut(s) 556
BmuI ACTGGG 1 cut(s) 556
BpiI GAAGAC 2 cut(s) 117, 930
Bpu10I CCTNAGC 2 cut(s) 446, 455
BpuEI CTTGAG 1 cut(s) 107
BpuMI CCSGG 1 cut(s) 934
BsaHI GRCGYC 2 cut(s) 434, 765
BsaJI CCNNGG 2 cut(s) 972, 980
BsaXI ACNNNNNCTCC 6 cut(s) 133, 163, 233, 263, 314, 344
Bsc4I CCNNNNNNNGG 4 cut(s) 65, 311, 597, 862
Bse118I RCCGGY 2 cut(s) 868, 904
Bse1I ACTGG 2 cut(s) 364, 551
BseBI CCWGG 2 cut(s) 592, 679
BseDI CCNNGG 2 cut(s) 972, 980
BseGI GGATG 1 cut(s) 468
BseLI CCNNNNNNNGG 4 cut(s) 65, 311, 597, 862
BseMII CTCAG 8 cut(s) 371, 440, 460, 469, 555, 676, 771, 818
BseNI ACTGG 2 cut(s) 364, 551
BseRI GAGGAG 2 cut(s) 164, 322
BseXI GCAGC 2 cut(s) 17, 307
BshFI GGCC 1 cut(s) 797
BsiSI CCGG 4 cut(s) 863, 869, 905, 933
BslI CCNNNNNNNGG 4 cut(s) 65, 311, 597, 862
BsmAI GTCTC 2 cut(s) 372, 441
BsmBI CGTCTC 1 cut(s) 441
BsmI GAATGC 1 cut(s) 751
BsnI GGCC 1 cut(s) 797
Bsp1286I GDGCHC 1 cut(s) 491
Bsp143I GATC 8 cut(s) 32, 55, 339, 348, 360, 567, 952, 1053
Bsp19I CCATGG 2 cut(s) 972, 980
BspANI GGCC 1 cut(s) 797
BspCNI CTCAG 8 cut(s) 370, 439, 459, 468, 556, 675, 770, 817
BspLI GGNNCC 4 cut(s) 575, 712, 867, 978
BspMI ACCTGC 1 cut(s) 472
BspPI GGATC 5 cut(s) 50, 334, 343, 355, 1048
BsrFI RCCGGY 2 cut(s) 868, 904
BsrI ACTGG 2 cut(s) 364, 551
BssAI RCCGGY 2 cut(s) 868, 904
BssECI CCNNGG 2 cut(s) 972, 980
BssMI GATC 8 cut(s) 32, 55, 339, 348, 360, 567, 952, 1053
BssNI GRCGYC 2 cut(s) 434, 765
BssT1I CCWWGG 2 cut(s) 972, 980
Bst2UI CCWGG 2 cut(s) 592, 679
Bst4CI ACNGT 2 cut(s) 26, 245
Bst6I CTCTTC 1 cut(s) 262
BstACI GRCGYC 2 cut(s) 434, 765
BstC8I GCNNGC 2 cut(s) 831, 853
BstDSI CCRYGG 2 cut(s) 972, 980
BstF5I GGATG 1 cut(s) 468
BstH2I RGCGCY 1 cut(s) 999
BstHHI GCGC 1 cut(s) 998
BstKTI GATC 8 cut(s) 35, 58, 342, 351, 363, 570, 955, 1056
BstMAI GTCTC 2 cut(s) 372, 441
BstMBI GATC 8 cut(s) 32, 55, 339, 348, 360, 567, 952, 1053
BstMWI GCNNNNNNNGC 5 cut(s) 301, 456, 626, 635, 732
BstNI CCWGG 2 cut(s) 592, 679
BstSCI CCNGG 3 cut(s) 590, 677, 932
BstSFI CTRYAG 1 cut(s) 22
BstV1I GCAGC 2 cut(s) 17, 307
BstV2I GAAGAC 2 cut(s) 117, 930
BstX2I RGATCY 4 cut(s) 55, 339, 348, 360
BstYI RGATCY 4 cut(s) 55, 339, 348, 360
BsuI GTATCC 1 cut(s) 1038
BsuRI GGCC 1 cut(s) 797
BtgI CCRYGG 2 cut(s) 972, 980
BtgZI GCGATG 1 cut(s) 99
BtsCI GGATG 1 cut(s) 468
BtsIMutI CAGTG 2 cut(s) 31, 544
BveI ACCTGC 1 cut(s) 472
Cac8I GCNNGC 2 cut(s) 831, 853
CaiI CAGNNNCTG 1 cut(s) 597
CfoI GCGC 1 cut(s) 998
Cfr10I RCCGGY 2 cut(s) 868, 904
Cfr13I GGNCC 2 cut(s) 711, 840
CseI GACGC 1 cut(s) 773
Csp6I GTAC 3 cut(s) 441, 655, 739
CspCI CAANNNNNGTGG 2 cut(s) 227, 262
CviAII CATG 2 cut(s) 973, 981
CviQI GTAC 3 cut(s) 441, 655, 739
DpnI GATC 8 cut(s) 34, 57, 341, 350, 362, 569, 954, 1055
DpnII GATC 8 cut(s) 32, 55, 339, 348, 360, 567, 952, 1053
EaeI YGGCCR 1 cut(s) 795
Eam1104I CTCTTC 1 cut(s) 262
EarI CTCTTC 1 cut(s) 262
EciI GGCGGA 4 cut(s) 154, 635, 689, 835
Eco130I CCWWGG 2 cut(s) 972, 980
Eco24I GRGCYC 1 cut(s) 491
Eco47I GGWCC 2 cut(s) 711, 840
EcoO109I RGGNCCY 1 cut(s) 840
EcoRII CCWGG 2 cut(s) 590, 677
EcoT14I CCWWGG 2 cut(s) 972, 980
EcoT38I GRGCYC 1 cut(s) 491
ErhI CCWWGG 2 cut(s) 972, 980
Esp3I CGTCTC 1 cut(s) 441
FaeI CATG 2 cut(s) 976, 984
FaiI YATR 4 cut(s) 98, 263, 974, 982
FalI AAGNNNNNCTT 2 cut(s) 94, 126
FatI CATG 2 cut(s) 972, 980
FauI CCCGC 1 cut(s) 699
FblI GTMKAC 1 cut(s) 762
Fnu4HI GCNGC 9 cut(s) 6, 119, 296, 503, 630, 651, 849, 944, 994
FokI GGATG 1 cut(s) 455
FriOI GRGCYC 1 cut(s) 491
Fsp4HI GCNGC 9 cut(s) 6, 119, 296, 503, 630, 651, 849, 944, 994
GlaI GCGC 1 cut(s) 997
GluI GCNGC 9 cut(s) 6, 119, 296, 503, 630, 651, 849, 944, 994
HaeII RGCGCY 1 cut(s) 999
HaeIII GGCC 1 cut(s) 797
HapII CCGG 4 cut(s) 863, 869, 905, 933
HgaI GACGC 1 cut(s) 773
HhaI GCGC 1 cut(s) 998
Hin1I GRCGYC 2 cut(s) 434, 765
Hin1II CATG 2 cut(s) 976, 984
Hin6I GCGC 1 cut(s) 996
HinP1I GCGC 1 cut(s) 996
HincII GTYRAC 1 cut(s) 763
HindII GTYRAC 1 cut(s) 763
HindIII AAGCTT 2 cut(s) 518, 770
HinfI GANTC 1 cut(s) 968
HpaII CCGG 4 cut(s) 863, 869, 905, 933
HphI GGTGA 4 cut(s) 80, 319, 883, 919
Hpy166II GTNNAC 1 cut(s) 763
Hpy188III TCNNGA 1 cut(s) 472
Hpy8I GTNNAC 1 cut(s) 763
Hpy99I CGWCG 5 cut(s) 245, 436, 719, 767, 904
HpyAV CCTTC 4 cut(s) 140, 162, 189, 782
HpyCH4III ACNGT 2 cut(s) 26, 245
HpyCH4IV ACGT 1 cut(s) 434
HpyCH4V TGCA 1 cut(s) 751
HpyF10VI GCNNNNNNNGC 5 cut(s) 301, 456, 626, 635, 732
HpySE526I ACGT 1 cut(s) 434
Hsp92I GRCGYC 2 cut(s) 434, 765
Hsp92II CATG 2 cut(s) 976, 984
HspAI GCGC 1 cut(s) 996
Kzo9I GATC 8 cut(s) 32, 55, 339, 348, 360, 567, 952, 1053
LmnI GCTCC 4 cut(s) 254, 579, 865, 976
Lsp1109I GCAGC 2 cut(s) 17, 307
MaeII ACGT 1 cut(s) 434
MaeIII GTNAC 2 cut(s) 871, 907
MalI GATC 8 cut(s) 34, 57, 341, 350, 362, 569, 954, 1055
MboI GATC 8 cut(s) 32, 55, 339, 348, 360, 567, 952, 1053
MboII GAAGA 5 cut(s) 32, 102, 117, 249, 930
MflI RGATCY 4 cut(s) 55, 339, 348, 360
MhlI GDGCHC 1 cut(s) 491
MluCI AATT 2 cut(s) 229, 685
MlyI GAGTC 1 cut(s) 962
MmeI TCCRAC 2 cut(s) 700, 999
MseI TTAA 1 cut(s) 1063
MslI CAYNNNNRTG 1 cut(s) 473
MspA1I CMGCKG 2 cut(s) 946, 993
MspI CCGG 4 cut(s) 863, 869, 905, 933
MspR9I CCNGG 3 cut(s) 592, 679, 934
Mva1269I GAATGC 1 cut(s) 751
MvaI CCWGG 2 cut(s) 592, 679
MwoI GCNNNNNNNGC 5 cut(s) 301, 456, 626, 635, 732
NciI CCSGG 1 cut(s) 934
NcoI CCATGG 2 cut(s) 972, 980
NdeII GATC 8 cut(s) 32, 55, 339, 348, 360, 567, 952, 1053
NlaIII CATG 2 cut(s) 976, 984
NlaIV GGNNCC 4 cut(s) 575, 712, 867, 978
NmeAIII GCCGAG 1 cut(s) 470
NmuCI GTSAC 2 cut(s) 871, 907
PaqCI CACCTGC 1 cut(s) 472
PcsI WCGNNNNNNNCGW 4 cut(s) 609, 714, 723, 908
PctI GAATGC 1 cut(s) 751
PflFI GACNNNGTC 1 cut(s) 433
PflMI CCANNNNNTGG 1 cut(s) 597
PkrI GCNGC 9 cut(s) 7, 120, 297, 504, 631, 652, 850, 945, 995
PleI GAGTC 1 cut(s) 962
PpsI GAGTC 1 cut(s) 962
PpuMI RGGWCCY 1 cut(s) 840
Psp5II RGGWCCY 1 cut(s) 840
Psp6I CCWGG 2 cut(s) 590, 677
PspGI CCWGG 2 cut(s) 590, 677
PspN4I GGNNCC 4 cut(s) 575, 712, 867, 978
PspPI GGNCC 2 cut(s) 711, 840
PspPPI RGGWCCY 1 cut(s) 840
PstNI CAGNNNCTG 1 cut(s) 597
PsuI RGATCY 4 cut(s) 55, 339, 348, 360
PsyI GACNNNGTC 1 cut(s) 433
RsaI GTAC 3 cut(s) 442, 656, 740
RsaNI GTAC 3 cut(s) 441, 655, 739
RseI CAYNNNNRTG 1 cut(s) 473
SalI GTCGAC 1 cut(s) 761
SaqAI TTAA 1 cut(s) 1063
SatI GCNGC 9 cut(s) 6, 119, 296, 503, 630, 651, 849, 944, 994
Sau3AI GATC 8 cut(s) 32, 55, 339, 348, 360, 567, 952, 1053
Sau96I GGNCC 2 cut(s) 711, 840
SchI GAGTC 1 cut(s) 962
ScrFI CCNGG 3 cut(s) 592, 679, 934
SduI GDGCHC 1 cut(s) 491
SfcI CTRYAG 1 cut(s) 22
SgrAI CRCCGGYG 1 cut(s) 904
SinI GGWCC 2 cut(s) 711, 840
SmiMI CAYNNNNRTG 1 cut(s) 473
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
Sse9I AATT 2 cut(s) 229, 685
StyD4I CCNGG 3 cut(s) 590, 677, 932
StyI CCWWGG 2 cut(s) 972, 980
TaaI ACNGT 2 cut(s) 26, 245
TaiI ACGT 1 cut(s) 437
TaqI TCGA 3 cut(s) 612, 762, 1052
TasI AATT 2 cut(s) 229, 685
TatI WGTACW 1 cut(s) 738
TauI GCSGC 7 cut(s) 121, 505, 632, 653, 851, 946, 996
Tru1I TTAA 1 cut(s) 1063
Tru9I TTAA 1 cut(s) 1063
TscAI CASTG 2 cut(s) 31, 551
TseFI GTSAC 2 cut(s) 871, 907
TseI GCWGC 2 cut(s) 5, 295
Tsp45I GTSAC 2 cut(s) 871, 907
TspDTI ATGAA 3 cut(s) 102, 113, 491
TspGWI ACGGA 3 cut(s) 703, 1003, 1020
TspRI CASTG 2 cut(s) 31, 551
Tth111I GACNNNGTC 1 cut(s) 433
Van91I CCANNNNNTGG 1 cut(s) 597
VpaK11BI GGWCC 2 cut(s) 711, 840
XmiI GTMKAC 1 cut(s) 762
ZraI GACGTC 1 cut(s) 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.