MD08G1181100.v1.1

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011007
Physical Location & Seq
Forward (+)
22482446 .. 22483876
1431 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1181100.v1.1.491

Sequence Viewer

Length: 1254 bp
ATGATAAAGACACCCATGGATTCTGGTAATAGTGGAAGTATGCAATCTTCAAGTGGTGGTGAAGCTCAAGACCATGAAATTTCTAGACCCGATTCGATCCCGGTTTACTTGAATTCTCCGACCGGCCACTTATTTGGTTCCTCCGACCCAACAGTACACCCATCTCTCCTTTCTCAATACCAAAACCACCCACCCACTACCACAACACTTTTTGATCATCATCTCTCATCTAGTAATTACACTTATGATCATTTCCACACAACATTGTCTCATCCCCAACCAAACTCATCAAACCCAAATTTCATCCCCAGCCCAAGATCCAATCAACCCAACTCCACCAACCCCATTCCCAACCCACAGCCTTCATCAACTACTACCGAAGCACGAGGAGGACAGACCAACACCGTTGCCACCCGGAACACGAAGAAAAGGACAAGAGCTTCTAGAAGGGCACCAACCACCGTTCTCACCACCGACACGTCTAATTTTCGGGCAATGGTGCAGGAGTTCACTGGTATACCAGCACCCCCATTTTCAGCTTCATCCTCATCACCATACGCTCGAAGACTCGAAATGTTTGGCTCCGGAATAAGGTCACCTCTTTACCCTTTACGTCCTTCGGCTCAAAAGGTCCATCAGCCAACCCCATTTCTCTCTCCTTCTTCTAATTCTAATACTTCATTGTTGATGATGATGAGCAATAGTATTAGTACTATGGTTGATGCTACTAATATTGCTACTACTAGTAATAATAGTAATGTTAATTTCAGTTCAATTAATTACCAATTACTATCTGATCATCATCAAAATCAAGGCCTCTTCCAAAACATGCAAAACCCTATTCTCACATTCCAATCCCTACCACAGCAGCCTCCTCCATTCCATTCTTCAATCAATGTGCCTAATTTTGGGGCAAGACCTACCCCCCGGGGAGTAATTGATAATTTGGCCATGCATTCATCATTTGAGGAGCAATTGGGTCCGAACCGTCATGGATCGTCACCATATGTTAACACAAACCAGCTCGGTGGTGATGGTGTTGGATCAATAGACGACAGCCATGTAGCAGCTTCAGATGGGAATGGCGGCTGGAGGGATCATGGAGTACTTGGATCAAGGGATGGGGGATTATCATCACATGATCACCATTTGAGGCCTAGTTTAGACAAGTGTTTGGAGATGGACAATGTTAATTCTAGTACTAGAGGTGAAGGTGCGGTGGACTCGTGGATTTCTCCTAATTCAGATCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VQ

Protein Analysis

418

Amino Acids

45.07

Weight (kDa)

6.48

Isoelectric Point (pI)

51.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 150 - 177 2.5e-12 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011007)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G35830 AT4G39720 AT4G39720 AT4G39720 AT4G39720
fragaria_vesca FvH4_5g37690
malus_domestica MD08G1181100.v1.1 MD15G1366700.v1.1
prunus_persica Prupe.1G514600_v2.0.a1
pyrus_communis pycom08g15480
rosa_chinensis RchiOBHm_Chr7g0239391
rosa_laevigata RLG00000000760
rosa_multiflora Rmu_sc0008456.1_g000009
rosa_roxburghii Rroxscaffold_3G00220970
rosa_rugosa Rorug07G0327800
rosa_samantha Rh7AG481000 Rh7BG453700 Rh7CG499000 Rh7DG467100
rosa_wichuraiana Rw7G040310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 451
AccI GTMKAC 1 cut(s) 517
AccIII TCCGGA 1 cut(s) 584
AciI CCGC 2 cut(s) 1086, 1217
AclWI GGATC 6 cut(s) 91, 312, 1003, 1051, 1104, 1120
AcoI YGGCCR 2 cut(s) 124, 948
AcsI RAATTY 3 cut(s) 78, 112, 298
AcuI CTGAAG 1 cut(s) 1056
AfaI GTAC 4 cut(s) 156, 712, 1107, 1201
AfiI CCNNNNNNNGG 2 cut(s) 591, 908
AflIII ACRYGT 1 cut(s) 477
AgsI TTSAA 4 cut(s) 51, 112, 774, 891
AhlI ACTAGT 1 cut(s) 743
AjiI CACGTC 1 cut(s) 480
AluBI AGCT 5 cut(s) 65, 440, 539, 1024, 1070
AluI AGCT 5 cut(s) 65, 440, 539, 1024, 1070
Alw26I GTCTC 1 cut(s) 273
AlwI GGATC 6 cut(s) 91, 312, 1003, 1051, 1104, 1120
Ama87I CYCGRG 1 cut(s) 927
Aor13HI TCCGGA 1 cut(s) 584
AoxI GGCC 4 cut(s) 124, 814, 948, 1154
ApeKI GCWGC 2 cut(s) 868, 1067
ApoI RAATTY 3 cut(s) 78, 112, 298
AseI ATTAAT 1 cut(s) 777
AspS9I GGNCC 2 cut(s) 631, 980
AsuC2I CCSGG 4 cut(s) 101, 415, 928, 929
AsuHPI GGTGA 8 cut(s) 71, 460, 543, 588, 993, 1043, 1136, 1220
AvaI CYCGRG 1 cut(s) 927
AvaII GGWCC 2 cut(s) 631, 980
BaeGI GKGCMC 1 cut(s) 454
BalI TGGCCA 1 cut(s) 950
BanI GGYRCC 1 cut(s) 451
BarI GAAGNNNNNNTAC 2 cut(s) 31, 63
BauI CACGAG 2 cut(s) 384, 1225
BbsI GAAGAC 1 cut(s) 571
BbvI GCAGC 2 cut(s) 880, 1079
BccI CCATC 6 cut(s) 169, 642, 1028, 1070, 1115, 1174
BclI TGATCA 4 cut(s) 214, 247, 796, 1141
BcnI CCSGG 4 cut(s) 101, 415, 928, 929
BcoDI GTCTC 1 cut(s) 273
BcuI ACTAGT 1 cut(s) 743
BfaI CTAG 8 cut(s) 84, 231, 444, 744, 1158, 1197, 1203, 1252
BisI GCNGC 3 cut(s) 869, 1068, 1087
BlsI GCNGC 3 cut(s) 870, 1069, 1088
BmcAI AGTACT 3 cut(s) 712, 1107, 1201
Bme1390I CCNGG 4 cut(s) 101, 415, 928, 929
Bme18I GGWCC 2 cut(s) 631, 980
BmeT110I CYCGRG 1 cut(s) 927
BmgBI CACGTC 1 cut(s) 480
BmgT120I GGNCC 2 cut(s) 631, 980
BmiI GGNNCC 4 cut(s) 139, 453, 583, 981
BmrFI CCNGG 4 cut(s) 101, 415, 928, 929
BmsI GCATC 1 cut(s) 712
BpiI GAAGAC 1 cut(s) 571
BpmI CTGGAG 1 cut(s) 1111
BpuEI CTTGAG 1 cut(s) 51
BpuMI CCSGG 4 cut(s) 101, 415, 928, 929
BsaBI GATNNNNATC 3 cut(s) 219, 801, 1132
BsaJI CCNNGG 4 cut(s) 15, 926, 927, 928
BsaWI WCCGGW 1 cut(s) 584
Bsc4I CCNNNNNNNGG 2 cut(s) 591, 908
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 1 cut(s) 517
Bse3DI GCAATG 1 cut(s) 501
Bse8I GATNNNNATC 3 cut(s) 219, 801, 1132
BseAI TCCGGA 1 cut(s) 584
BseDI CCNNGG 4 cut(s) 15, 926, 927, 928
BseGI GGATG 4 cut(s) 271, 303, 542, 1126
BseJI GATNNNNATC 3 cut(s) 219, 801, 1132
BseLI CCNNNNNNNGG 2 cut(s) 591, 908
BseMI GCAATG 1 cut(s) 501
BseNI ACTGG 1 cut(s) 517
BseRI GAGGAG 3 cut(s) 402, 864, 983
BseSI GKGCMC 1 cut(s) 454
BseXI GCAGC 2 cut(s) 880, 1079
BseYI CCCAGC 1 cut(s) 308
BsgI GTGCAG 1 cut(s) 521
Bsh1285I CGRYCG 1 cut(s) 123
BshFI GGCC 4 cut(s) 126, 816, 950, 1156
BshNI GGYRCC 1 cut(s) 451
BsiEI CGRYCG 1 cut(s) 123
BsiHKCI CYCGRG 1 cut(s) 927
BsiSI CCGG 5 cut(s) 101, 123, 415, 585, 928
BslI CCNNNNNNNGG 2 cut(s) 591, 908
BsmAI GTCTC 1 cut(s) 273
BsmI GAATGC 1 cut(s) 955
BsnI GGCC 4 cut(s) 126, 816, 950, 1156
BsoBI CYCGRG 1 cut(s) 927
Bsp1286I GDGCHC 1 cut(s) 454
Bsp13I TCCGGA 1 cut(s) 584
Bsp19I CCATGG 1 cut(s) 15
BspACI CCGC 2 cut(s) 1086, 1217
BspANI GGCC 4 cut(s) 126, 816, 950, 1156
BspEI TCCGGA 1 cut(s) 584
BspLI GGNNCC 4 cut(s) 139, 453, 583, 981
BspPI GGATC 6 cut(s) 91, 312, 1003, 1051, 1104, 1120
BspT107I GGYRCC 1 cut(s) 451
BsrDI GCAATG 1 cut(s) 501
BsrFI RCCGGY 1 cut(s) 122
BsrI ACTGG 1 cut(s) 517
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 4 cut(s) 15, 926, 927, 928
BssNAI GTATAC 1 cut(s) 518
BssSI CACGAG 2 cut(s) 384, 1225
BssT1I CCWWGG 1 cut(s) 15
Bst1107I GTATAC 1 cut(s) 518
Bst2BI CACGAG 2 cut(s) 384, 1225
Bst4CI ACNGT 4 cut(s) 154, 406, 463, 989
Bst6I CTCTTC 1 cut(s) 824
BstDSI CCRYGG 1 cut(s) 15
BstEII GGTNACC 1 cut(s) 594
BstF5I GGATG 4 cut(s) 271, 303, 542, 1126
BstMAI GTCTC 1 cut(s) 273
BstMCI CGRYCG 1 cut(s) 123
BstNSI RCATGY 1 cut(s) 832
BstPI GGTNACC 1 cut(s) 594
BstSCI CCNGG 4 cut(s) 99, 413, 926, 927
BstSLI GKGCMC 1 cut(s) 454
BstV1I GCAGC 2 cut(s) 880, 1079
BstV2I GAAGAC 1 cut(s) 571
BstX2I RGATCY 1 cut(s) 317
BstXI CCANNNNNNTGG 2 cut(s) 134, 1028
BstYI RGATCY 1 cut(s) 317
BstZ17I GTATAC 1 cut(s) 518
BsuRI GGCC 4 cut(s) 126, 816, 950, 1156
BtgI CCRYGG 1 cut(s) 15
BtrI CACGTC 1 cut(s) 480
BtsCI GGATG 4 cut(s) 271, 303, 542, 1126
BtsIMutI CAGTG 1 cut(s) 510
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 2 cut(s) 631, 980
Cfr9I CCCGGG 1 cut(s) 927
Csp6I GTAC 4 cut(s) 155, 711, 1106, 1200
CviAII CATG 8 cut(s) 16, 74, 829, 952, 992, 1061, 1100, 1139
CviQI GTAC 4 cut(s) 155, 711, 1106, 1200
EaeI YGGCCR 2 cut(s) 124, 948
Eam1104I CTCTTC 1 cut(s) 824
EarI CTCTTC 1 cut(s) 824
Eco130I CCWWGG 1 cut(s) 15
Eco147I AGGCCT 2 cut(s) 816, 1156
Eco47I GGWCC 2 cut(s) 631, 980
Eco57I CTGAAG 1 cut(s) 1056
Eco88I CYCGRG 1 cut(s) 927
Eco91I GGTNACC 1 cut(s) 594
EcoO65I GGTNACC 1 cut(s) 594
EcoRI GAATTC 1 cut(s) 112
EcoT14I CCWWGG 1 cut(s) 15
EcoT22I ATGCAT 1 cut(s) 957
ErhI CCWWGG 1 cut(s) 15
FaeI CATG 8 cut(s) 19, 77, 832, 955, 995, 1064, 1103, 1142
FatI CATG 8 cut(s) 15, 73, 828, 951, 991, 1060, 1099, 1138
FauNDI CATATG 1 cut(s) 1006
FbaI TGATCA 4 cut(s) 214, 247, 796, 1141
FblI GTMKAC 1 cut(s) 517
Fnu4HI GCNGC 3 cut(s) 869, 1068, 1087
FokI GGATG 4 cut(s) 258, 290, 529, 1133
Fsp4HI GCNGC 3 cut(s) 869, 1068, 1087
FspBI CTAG 8 cut(s) 84, 231, 444, 744, 1158, 1197, 1203, 1252
GluI GCNGC 3 cut(s) 869, 1068, 1087
GsaI CCCAGC 1 cut(s) 312
GsuI CTGGAG 1 cut(s) 1111
HaeIII GGCC 4 cut(s) 126, 816, 950, 1156
HapII CCGG 5 cut(s) 101, 123, 415, 585, 928
Hin1II CATG 8 cut(s) 19, 77, 832, 955, 995, 1064, 1103, 1142
HincII GTYRAC 1 cut(s) 1012
HindII GTYRAC 1 cut(s) 1012
HinfI GANTC 4 cut(s) 20, 92, 567, 1223
HpaI GTTAAC 1 cut(s) 1012
HpaII CCGG 5 cut(s) 101, 123, 415, 585, 928
HphI GGTGA 8 cut(s) 71, 460, 543, 588, 993, 1043, 1136, 1220
Hpy166II GTNNAC 6 cut(s) 106, 157, 510, 518, 1012, 1222
Hpy188I TCNGA 6 cut(s) 120, 145, 796, 984, 1075, 1246
Hpy188III TCNNGA 4 cut(s) 68, 84, 444, 585
Hpy8I GTNNAC 6 cut(s) 106, 157, 510, 518, 1012, 1222
HpyAV CCTTC 5 cut(s) 372, 441, 627, 669, 1205
HpyCH4III ACNGT 4 cut(s) 154, 406, 463, 989
HpyCH4IV ACGT 2 cut(s) 479, 613
HpyCH4V TGCA 4 cut(s) 43, 502, 832, 955
HpySE526I ACGT 2 cut(s) 479, 613
Hsp92II CATG 8 cut(s) 19, 77, 832, 955, 995, 1064, 1103, 1142
Kpn2I TCCGGA 1 cut(s) 584
Ksp22I TGATCA 4 cut(s) 214, 247, 796, 1141
KspAI GTTAAC 1 cut(s) 1012
LmnI GCTCC 2 cut(s) 587, 970
Lsp1109I GCAGC 2 cut(s) 880, 1079
LweI GCATC 1 cut(s) 712
MaeI CTAG 8 cut(s) 84, 231, 444, 744, 1158, 1197, 1203, 1252
MaeII ACGT 2 cut(s) 479, 613
MaeIII GTNAC 2 cut(s) 594, 999
MboII GAAGA 6 cut(s) 39, 436, 576, 654, 811, 879
MfeI CAATTG 1 cut(s) 974
MflI RGATCY 1 cut(s) 317
MhlI GDGCHC 1 cut(s) 454
MlsI TGGCCA 1 cut(s) 950
MluNI TGGCCA 1 cut(s) 950
MlyI GAGTC 2 cut(s) 561, 1217
MmeI TCCRAC 3 cut(s) 143, 168, 1021
Mox20I TGGCCA 1 cut(s) 950
Mph1103I ATGCAT 1 cut(s) 957
MroI TCCGGA 1 cut(s) 584
MscI TGGCCA 1 cut(s) 950
MseI TTAA 4 cut(s) 762, 777, 1011, 1191
Msp20I TGGCCA 1 cut(s) 950
MspI CCGG 5 cut(s) 101, 123, 415, 585, 928
MspR9I CCNGG 4 cut(s) 101, 415, 928, 929
MunI CAATTG 1 cut(s) 974
Mva1269I GAATGC 1 cut(s) 955
NciI CCSGG 4 cut(s) 101, 415, 928, 929
NcoI CCATGG 1 cut(s) 15
NdeI CATATG 1 cut(s) 1006
NlaIII CATG 8 cut(s) 19, 77, 832, 955, 995, 1064, 1103, 1142
NlaIV GGNNCC 4 cut(s) 139, 453, 583, 981
NmuCI GTSAC 2 cut(s) 594, 999
NsiI ATGCAT 1 cut(s) 957
NspI RCATGY 1 cut(s) 832
PceI AGGCCT 2 cut(s) 816, 1156
PctI GAATGC 1 cut(s) 955
PfeI GAWTC 2 cut(s) 20, 92
PkrI GCNGC 3 cut(s) 870, 1069, 1088
PleI GAGTC 2 cut(s) 561, 1217
PpsI GAGTC 2 cut(s) 561, 1217
PshBI ATTAAT 1 cut(s) 777
PspEI GGTNACC 1 cut(s) 594
PspFI CCCAGC 1 cut(s) 308
PspN4I GGNNCC 4 cut(s) 139, 453, 583, 981
PspPI GGNCC 2 cut(s) 631, 980
PsuI RGATCY 1 cut(s) 317
RsaI GTAC 4 cut(s) 156, 712, 1107, 1201
RsaNI GTAC 4 cut(s) 155, 711, 1106, 1200
SaqAI TTAA 4 cut(s) 762, 777, 1011, 1191
SatI GCNGC 3 cut(s) 869, 1068, 1087
Sau96I GGNCC 2 cut(s) 631, 980
ScaI AGTACT 3 cut(s) 712, 1107, 1201
SchI GAGTC 2 cut(s) 561, 1217
ScrFI CCNGG 4 cut(s) 101, 415, 928, 929
SduI GDGCHC 1 cut(s) 454
SfaNI GCATC 1 cut(s) 712
SinI GGWCC 2 cut(s) 631, 980
SmaI CCCGGG 1 cut(s) 929
SmlI CTYRAG 1 cut(s) 66
SmoI CTYRAG 1 cut(s) 66
SpeI ACTAGT 1 cut(s) 743
SseBI AGGCCT 2 cut(s) 816, 1156
SsiI CCGC 2 cut(s) 1086, 1217
SspI AATATT 1 cut(s) 733
SspMI CTAG 8 cut(s) 84, 231, 444, 744, 1158, 1197, 1203, 1252
StuI AGGCCT 2 cut(s) 816, 1156
StyD4I CCNGG 4 cut(s) 99, 413, 926, 927
StyI CCWWGG 1 cut(s) 15
TaaI ACNGT 4 cut(s) 154, 406, 463, 989
TaiI ACGT 2 cut(s) 482, 616
TaqI TCGA 3 cut(s) 95, 562, 570
TatI WGTACW 4 cut(s) 154, 710, 1105, 1199
TauI GCSGC 1 cut(s) 1089
TfiI GAWTC 2 cut(s) 20, 92
Tru1I TTAA 4 cut(s) 762, 777, 1011, 1191
Tru9I TTAA 4 cut(s) 762, 777, 1011, 1191
TscAI CASTG 1 cut(s) 517
TseFI GTSAC 2 cut(s) 594, 999
TseI GCWGC 2 cut(s) 868, 1067
Tsp45I GTSAC 2 cut(s) 594, 999
TspDTI ATGAA 6 cut(s) 90, 292, 354, 531, 669, 948
TspMI CCCGGG 1 cut(s) 927
TspRI CASTG 1 cut(s) 517
VpaK11BI GGWCC 2 cut(s) 631, 980
VspI ATTAAT 1 cut(s) 777
XapI RAATTY 3 cut(s) 78, 112, 298
XbaI TCTAGA 2 cut(s) 83, 443
XceI RCATGY 1 cut(s) 832
XmaI CCCGGG 1 cut(s) 927
XmiI GTMKAC 1 cut(s) 517
XspI CTAG 8 cut(s) 84, 231, 444, 744, 1158, 1197, 1203, 1252
ZrmI AGTACT 3 cut(s) 712, 1107, 1201
Zsp2I ATGCAT 1 cut(s) 957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.