MD08G1216300.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012017
Physical Location & Seq
Forward (+)
27906753 .. 27910956
4204 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1216300.v1.1.491

Sequence Viewer

Length: 1515 bp
ATGGCATTATTCAGGTACGTCTCCTCGCAAAACCGAAAGTACCCACCTTTAGTTTTGTCTCTCTCTCGCTCTCTCTGCTCTGAAAGCGGCAGCTCAACCACCGCCGCCGACGTCGAGACTGTTTATCGCATACTCAGTTGCCCCACTGGTTCCAAAAACTTGAAACAGGCATTGAAATCGAGTGGGGTTTTTCTCTCCAATGATTTGATTGATAAGGTTCTCAAACGGGTTCGGTTCAGCCATGGAAACCCTTCACAGGCTTTGGAGTTTTTCAATTACACCGGCAACAGAAGAGGGTTTTACCACACTCAGTTTTCCTTGGACACAATGCTTTACATTCTAGGACGAAGTCGAATGTTCGACAAAACTTGGGAGGTCCTAGTTGACATTAAGTATAAGGATCGAAATTTGATAACCCCGCGCACTGTAATGGTTGTGTTAGCTAGAATCGCTAAGGTTTGCTCCGTTAGGCAAACCGTTGAGTCTTTTAGGAAGTTCAAGAAGCTCGTGCCTGAATTCGATACAATGTGTTTCAATTCCTTGTTGAGGACTCTTTGTCAGGAGAAGAGTATGGCGGATGCGAGGAATGTGTATCACAGTCTCAAGCAAAATTTTAAGCCCAATTTGCAAACTTTCAATATATTGCTGTCCGGGTGGAAGACATCCGAGGAAGCTGAGGGGTTCTTTGAGGAGATGAGGGAGATGGGTGTGAAACCTGATGTCGTTTCCTACAATTGTTTGGTTGATGTTTATTGTAAGAATAGAGAAGTAGACAAGGCATTTAAGATTGTAGAGGAAATGCGAGAACAGGATATATCACCTGATGTGATCACCTATACTAGTATCATTGGTGGATTGGGGTTGGTTGGTCAGCCCGATAAAGCCAGAGATCTATTGAAGGAAATGACGGAGTATGGGTGCTACCCAGATGCTGCTGCATACAATGCGGCCATTAGGAATTTTTGCATCGCGAAGAGGCTTGGCGATGCTTATGGGTTGATGGATGCAATGGCGAGTAAGGGTTTGAGTCCGAATGCAACTACTTACAATTTGTTCTTCAGGGTTTTCTTCTGGTCAAATGACTTACAAGGCTCTTGGAGTTTGTATGGGAGAATGATGCATGCTGGGTGCTTACCAAGTACACAGTCTTGTATGTTCCTGATCAGGTTGTTTCAGAGACAGGAAAAGGTGGATATGGCATTGCGGCTGTGGAATGATATGATTGAGAAGGGTTTCGGTTCTTATATATTGGTATCTGATGTGCTGTTTGATTTGCTTTGTGATTTGGGAAAACTAGTGGAAGCAGAGAGGTGCTTCTTGCAGATGGTAGAAAAGGGGCAAAAGCCTAGTAGCGTTTCCTTCAGAAGGATCAAGGTGCTTATGGAACTGGCAAACAAGCATGAGGCCCTCAAGAACTTGACCGAGAAAATGGCAGTGTTCGGTGCTTCAATCCATCTTCCTGAGAATTCAAAGAGCTCAATGAATACTTCGCGTTTACAATCACATCCAATATAA

Protein Analysis

505

Amino Acids

57.49

Weight (kDa)

9.09

Isoelectric Point (pI)

38.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 174 - 218 2.3e-07 PPR repeat family
PPR_long PF17177 216 - 279 6.7e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 229 - 285 6.2e-14 Pentatricopeptide repeat domain
TPR_24 PF23276 231 - 331 3.5e-08 Fungal tetratrico peptide repeats
PPR_1 PF12854 235 - 268 3e-13 PPR repeat
PPR_2 PF13041 239 - 286 1.4e-17 PPR repeat family
PPR PF01535 242 - 271 5.2e-08 PPR repeat
PPR_long PF17177 258 - 355 8.1e-09 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 271 - 303 4.2e-07 PPR repeat
PPR PF01535 277 - 307 2.4e-06 PPR repeat
PPR_2 PF13041 282 - 322 3.8e-06 PPR repeat family
PPR_2 PF13041 309 - 356 4.4e-09 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012017)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02420
fragaria_vesca FvH4_5g32610 FvH4_5g32610 FvH4_5g32610
malus_domestica MD08G1216300.v1.1
prunus_persica Prupe.1G549200_v2.0.a1
pyrus_communis pycom08g18730
rosa_chinensis RchiOBHm_Chr7g0230241
rosa_laevigata RLG00000001507
rosa_multiflora Rmu_sc0002822.1_g000019 Rmu_sc0008506.1_g000024
rosa_roxburghii Rroxscaffold_3G00230280
rosa_rugosa Rorug07G0260000
rosa_samantha Rh7AG409400 Rh7BG389300 Rh7CG428000 Rh7DG407000
rosa_wichuraiana Rw0G018590 Rw0G022620

Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 114
AccI GTMKAC 1 cut(s) 771
AccII CGCG 3 cut(s) 421, 971, 1492
AciI CCGC 7 cut(s) 87, 102, 105, 419, 575, 947, 1204
AclWI GGATC 2 cut(s) 408, 1376
AcoI YGGCCR 1 cut(s) 948
AcsI RAATTY 5 cut(s) 406, 515, 610, 958, 1465
AcuI CTGAAG 2 cut(s) 1042, 1345
AcyI GRCGYC 1 cut(s) 111
AfaI GTAC 3 cut(s) 17, 41, 1141
AfiI CCNNNNNNNGG 3 cut(s) 256, 546, 1365
AgsI TTSAA 9 cut(s) 163, 175, 274, 499, 535, 637, 898, 1449, 1470
AhdI GACNNNNNGTC 1 cut(s) 555
AhlI ACTAGT 2 cut(s) 839, 1294
AluBI AGCT 5 cut(s) 93, 443, 505, 674, 1476
AluI AGCT 5 cut(s) 93, 443, 505, 674, 1476
Alw21I GWGCWC 1 cut(s) 1478
Alw26I GTCTC 5 cut(s) 25, 63, 110, 605, 1171
AlwI GGATC 2 cut(s) 408, 1376
AlwNI CAGNNNCTG 1 cut(s) 932
AoxI GGCC 2 cut(s) 948, 1404
ApeKI GCWGC 3 cut(s) 90, 932, 935
ApoI RAATTY 5 cut(s) 406, 515, 610, 958, 1465
Asp700I GAANNNNTTC 2 cut(s) 250, 1232
AspLEI GCGC 1 cut(s) 423
AspS9I GGNCC 2 cut(s) 376, 1405
AsuC2I CCSGG 1 cut(s) 652
AsuHPI GGTGA 2 cut(s) 810, 823
AvaII GGWCC 1 cut(s) 376
BanII GRGCYC 1 cut(s) 1478
BauI CACGAG 1 cut(s) 506
BbsI GAAGAC 1 cut(s) 665
Bbv12I GWGCWC 1 cut(s) 1478
BbvCI CCTCAGC 1 cut(s) 675
BbvI GCAGC 3 cut(s) 102, 919, 922
BccI CCATC 4 cut(s) 697, 994, 1318, 1461
BcgI CGANNNNNNTGC 2 cut(s) 159, 193
BclI TGATCA 2 cut(s) 828, 1161
BcnI CCSGG 1 cut(s) 652
BcoDI GTCTC 5 cut(s) 25, 63, 110, 605, 1171
BcuI ACTAGT 2 cut(s) 839, 1294
BfaI CTAG 6 cut(s) 341, 380, 444, 840, 1295, 1347
BglII AGATCT 1 cut(s) 889
BisI GCNGC 7 cut(s) 88, 91, 105, 933, 936, 948, 1205
BlsI GCNGC 7 cut(s) 89, 92, 106, 934, 937, 949, 1206
Bme1390I CCNGG 1 cut(s) 652
Bme18I GGWCC 1 cut(s) 376
BmeRI GACNNNNNGTC 1 cut(s) 555
BmgT120I GGNCC 2 cut(s) 376, 1405
BmiI GGNNCC 1 cut(s) 151
BmrFI CCNGG 1 cut(s) 652
BmsI GCATC 6 cut(s) 568, 919, 975, 976, 994, 1107
BpiI GAAGAC 1 cut(s) 665
Bpu10I CCTNAGC 2 cut(s) 453, 675
BpuEI CTTGAG 2 cut(s) 587, 1394
BpuMI CCSGG 1 cut(s) 652
BsaHI GRCGYC 1 cut(s) 111
BsaJI CCNNGG 3 cut(s) 241, 318, 666
BsaXI ACNNNNNCTCC 2 cut(s) 902, 932
Bsc4I CCNNNNNNNGG 3 cut(s) 256, 546, 1365
Bse118I RCCGGY 1 cut(s) 281
Bse1I ACTGG 2 cut(s) 151, 1392
Bse3DI GCAATG 2 cut(s) 1014, 1199
BseDI CCNNGG 3 cut(s) 241, 318, 666
BseGI GGATG 4 cut(s) 583, 662, 1009, 1504
BseLI CCNNNNNNNGG 3 cut(s) 256, 546, 1365
BseMI GCAATG 2 cut(s) 1014, 1199
BseMII CTCAG 4 cut(s) 148, 323, 666, 1452
BseNI ACTGG 2 cut(s) 151, 1392
BseRI GAGGAG 2 cut(s) 13, 704
BseXI GCAGC 3 cut(s) 102, 919, 922
BseYI CCCAGC 1 cut(s) 1124
Bsh1236I CGCG 3 cut(s) 421, 971, 1492
BshFI GGCC 2 cut(s) 950, 1406
BsiHKAI GWGCWC 1 cut(s) 1478
BsiSI CCGG 2 cut(s) 282, 651
BslI CCNNNNNNNGG 3 cut(s) 256, 546, 1365
BsmAI GTCTC 5 cut(s) 25, 63, 110, 605, 1171
BsmBI CGTCTC 1 cut(s) 25
BsmI GAATGC 1 cut(s) 1039
BsnI GGCC 2 cut(s) 950, 1406
Bsp1286I GDGCHC 1 cut(s) 1478
Bsp143I GATC 5 cut(s) 400, 828, 889, 1161, 1368
Bsp19I CCATGG 1 cut(s) 241
Bsp68I TCGCGA 1 cut(s) 971
BspACI CCGC 7 cut(s) 87, 102, 105, 419, 575, 947, 1204
BspANI GGCC 2 cut(s) 950, 1406
BspCNI CTCAG 4 cut(s) 147, 322, 667, 1453
BspFNI CGCG 3 cut(s) 421, 971, 1492
BspLI GGNNCC 1 cut(s) 151
BspPI GGATC 2 cut(s) 408, 1376
BsrDI GCAATG 2 cut(s) 1014, 1199
BsrFI RCCGGY 1 cut(s) 281
BsrI ACTGG 2 cut(s) 151, 1392
BssAI RCCGGY 1 cut(s) 281
BssECI CCNNGG 3 cut(s) 241, 318, 666
BssMI GATC 5 cut(s) 400, 828, 889, 1161, 1368
BssNI GRCGYC 1 cut(s) 111
BssSI CACGAG 1 cut(s) 506
BssT1I CCWWGG 2 cut(s) 241, 318
Bst2BI CACGAG 1 cut(s) 506
Bst4CI ACNGT 5 cut(s) 121, 427, 478, 599, 1146
Bst6I CTCTTC 3 cut(s) 286, 560, 968
BstACI GRCGYC 1 cut(s) 111
BstAPI GCANNNNNTGC 1 cut(s) 944
BstC8I GCNNGC 1 cut(s) 1122
BstDEI CTNAG 5 cut(s) 134, 309, 453, 675, 1461
BstDSI CCRYGG 1 cut(s) 241
BstENI CCTNNNNNAGG 2 cut(s) 544, 1363
BstF5I GGATG 4 cut(s) 583, 662, 1009, 1504
BstFNI CGCG 3 cut(s) 421, 971, 1492
BstHHI GCGC 1 cut(s) 423
BstKTI GATC 5 cut(s) 403, 831, 892, 1164, 1371
BstMAI GTCTC 5 cut(s) 25, 63, 110, 605, 1171
BstMBI GATC 5 cut(s) 400, 828, 889, 1161, 1368
BstMWI GCNNNNNNNGC 5 cut(s) 75, 84, 449, 625, 944
BstNSI RCATGY 1 cut(s) 1124
BstSCI CCNGG 1 cut(s) 650
BstUI CGCG 3 cut(s) 421, 971, 1492
BstV1I GCAGC 3 cut(s) 102, 919, 922
BstV2I GAAGAC 1 cut(s) 665
BstX2I RGATCY 1 cut(s) 889
BstYI RGATCY 1 cut(s) 889
BsuRI GGCC 2 cut(s) 950, 1406
BtgI CCRYGG 1 cut(s) 241
BtgZI GCGATG 2 cut(s) 952, 999
BtsCI GGATG 4 cut(s) 583, 662, 1009, 1504
BtsI GCAGTG 1 cut(s) 1440
BtsIMutI CAGTG 3 cut(s) 144, 423, 1440
BtuMI TCGCGA 1 cut(s) 971
Cac8I GCNNGC 1 cut(s) 1122
CaiI CAGNNNCTG 1 cut(s) 932
CfoI GCGC 1 cut(s) 423
Cfr10I RCCGGY 1 cut(s) 281
Cfr13I GGNCC 2 cut(s) 376, 1405
Csp6I GTAC 3 cut(s) 16, 40, 1140
CviAII CATG 3 cut(s) 242, 1121, 1400
CviQI GTAC 3 cut(s) 16, 40, 1140
DdeI CTNAG 5 cut(s) 134, 309, 453, 675, 1461
DpnI GATC 5 cut(s) 402, 830, 891, 1163, 1370
DpnII GATC 5 cut(s) 400, 828, 889, 1161, 1368
DriI GACNNNNNGTC 1 cut(s) 555
EaeI YGGCCR 1 cut(s) 948
Eam1104I CTCTTC 3 cut(s) 286, 560, 968
Eam1105I GACNNNNNGTC 1 cut(s) 555
EarI CTCTTC 3 cut(s) 286, 560, 968
EciI GGCGGA 1 cut(s) 590
Ecl136II GAGCTC 1 cut(s) 1476
Eco130I CCWWGG 2 cut(s) 241, 318
Eco24I GRGCYC 1 cut(s) 1478
Eco47I GGWCC 1 cut(s) 376
Eco53kI GAGCTC 1 cut(s) 1476
Eco57I CTGAAG 2 cut(s) 1042, 1345
EcoICRI GAGCTC 1 cut(s) 1476
EcoNI CCTNNNNNAGG 2 cut(s) 544, 1363
EcoO109I RGGNCCY 2 cut(s) 376, 1405
EcoRI GAATTC 2 cut(s) 515, 1465
EcoT14I CCWWGG 2 cut(s) 241, 318
EcoT22I ATGCAT 1 cut(s) 1122
EcoT38I GRGCYC 1 cut(s) 1478
ErhI CCWWGG 2 cut(s) 241, 318
Esp3I CGTCTC 1 cut(s) 25
FaeI CATG 3 cut(s) 245, 1124, 1403
FatI CATG 3 cut(s) 241, 1120, 1399
FauI CCCGC 1 cut(s) 426
FbaI TGATCA 2 cut(s) 828, 1161
FblI GTMKAC 1 cut(s) 771
Fnu4HI GCNGC 7 cut(s) 88, 91, 105, 933, 936, 948, 1205
FokI GGATG 4 cut(s) 590, 649, 1016, 1491
FriOI GRGCYC 1 cut(s) 1478
Fsp4HI GCNGC 7 cut(s) 88, 91, 105, 933, 936, 948, 1205
FspBI CTAG 6 cut(s) 341, 380, 444, 840, 1295, 1347
GlaI GCGC 1 cut(s) 422
GluI GCNGC 7 cut(s) 88, 91, 105, 933, 936, 948, 1205
GsaI CCCAGC 1 cut(s) 1128
HaeIII GGCC 2 cut(s) 950, 1406
HapII CCGG 2 cut(s) 282, 651
HhaI GCGC 1 cut(s) 423
Hin1I GRCGYC 1 cut(s) 111
Hin1II CATG 3 cut(s) 245, 1124, 1403
Hin6I GCGC 1 cut(s) 421
HinP1I GCGC 1 cut(s) 421
HincII GTYRAC 1 cut(s) 385
HindII GTYRAC 1 cut(s) 385
HinfI GANTC 4 cut(s) 447, 482, 550, 1027
HpaII CCGG 2 cut(s) 282, 651
HphI GGTGA 2 cut(s) 810, 823
Hpy166II GTNNAC 4 cut(s) 385, 772, 1142, 1496
Hpy188I TCNGA 6 cut(s) 82, 667, 1032, 1176, 1258, 1364
Hpy188III TCNNGA 7 cut(s) 115, 499, 560, 970, 1159, 1411, 1460
Hpy8I GTNNAC 4 cut(s) 385, 772, 1142, 1496
Hpy99I CGWCG 2 cut(s) 113, 116
HpyAV CCTTC 5 cut(s) 261, 892, 1222, 1359, 1369
HpyCH4III ACNGT 5 cut(s) 121, 427, 478, 599, 1146
HpyCH4IV ACGT 2 cut(s) 18, 111
HpyCH4V TGCA 7 cut(s) 628, 938, 966, 1007, 1037, 1120, 1321
HpyF10VI GCNNNNNNNGC 5 cut(s) 75, 84, 449, 625, 944
HpyF3I CTNAG 5 cut(s) 134, 309, 453, 675, 1461
HpySE526I ACGT 2 cut(s) 18, 111
Hsp92I GRCGYC 1 cut(s) 111
Hsp92II CATG 3 cut(s) 245, 1124, 1403
HspAI GCGC 1 cut(s) 421
Ksp22I TGATCA 2 cut(s) 828, 1161
Kzo9I GATC 5 cut(s) 400, 828, 889, 1161, 1368
LmnI GCTCC 1 cut(s) 467
Lsp1109I GCAGC 3 cut(s) 102, 919, 922
LweI GCATC 6 cut(s) 568, 919, 975, 976, 994, 1107
MaeI CTAG 6 cut(s) 341, 380, 444, 840, 1295, 1347
MaeII ACGT 2 cut(s) 18, 111
MalI GATC 5 cut(s) 402, 830, 891, 1163, 1370
MboI GATC 5 cut(s) 400, 828, 889, 1161, 1368
MboII GAAGA 7 cut(s) 303, 577, 670, 985, 1048, 1060, 1448
MfeI CAATTG 1 cut(s) 733
MflI RGATCY 1 cut(s) 889
MhlI GDGCHC 1 cut(s) 1478
MlyI GAGTC 3 cut(s) 491, 544, 1036
Mph1103I ATGCAT 1 cut(s) 1122
MroXI GAANNNNTTC 2 cut(s) 250, 1232
MseI TTAA 3 cut(s) 390, 615, 783
MslI CAYNNNNRTG 1 cut(s) 428
MspI CCGG 2 cut(s) 282, 651
MspR9I CCNGG 1 cut(s) 652
MunI CAATTG 1 cut(s) 733
Mva1269I GAATGC 1 cut(s) 1039
MvnI CGCG 3 cut(s) 421, 971, 1492
MwoI GCNNNNNNNGC 5 cut(s) 75, 84, 449, 625, 944
NciI CCSGG 1 cut(s) 652
NcoI CCATGG 1 cut(s) 241
NdeII GATC 5 cut(s) 400, 828, 889, 1161, 1368
NlaIII CATG 3 cut(s) 245, 1124, 1403
NlaIV GGNNCC 1 cut(s) 151
NruI TCGCGA 1 cut(s) 971
NsiI ATGCAT 1 cut(s) 1122
NspI RCATGY 1 cut(s) 1124
PaeI GCATGC 1 cut(s) 1124
PctI GAATGC 1 cut(s) 1039
PdmI GAANNNNTTC 2 cut(s) 250, 1232
PfeI GAWTC 1 cut(s) 447
PflFI GACNNNGTC 1 cut(s) 348
PkrI GCNGC 7 cut(s) 89, 92, 106, 934, 937, 949, 1206
PleI GAGTC 3 cut(s) 490, 544, 1035
PpsI GAGTC 3 cut(s) 490, 544, 1035
PpuMI RGGWCCY 1 cut(s) 376
Psp124BI GAGCTC 1 cut(s) 1478
Psp5II RGGWCCY 1 cut(s) 376
PspFI CCCAGC 1 cut(s) 1124
PspN4I GGNNCC 1 cut(s) 151
PspPI GGNCC 2 cut(s) 376, 1405
PspPPI RGGWCCY 1 cut(s) 376
PstNI CAGNNNCTG 1 cut(s) 932
PsuI RGATCY 1 cut(s) 889
PsyI GACNNNGTC 1 cut(s) 348
RruI TCGCGA 1 cut(s) 971
RsaI GTAC 3 cut(s) 17, 41, 1141
RsaNI GTAC 3 cut(s) 16, 40, 1140
RseI CAYNNNNRTG 1 cut(s) 428
SacI GAGCTC 1 cut(s) 1478
SaqAI TTAA 3 cut(s) 390, 615, 783
SatI GCNGC 7 cut(s) 88, 91, 105, 933, 936, 948, 1205
Sau3AI GATC 5 cut(s) 400, 828, 889, 1161, 1368
Sau96I GGNCC 2 cut(s) 376, 1405
SchI GAGTC 3 cut(s) 491, 544, 1036
ScrFI CCNGG 1 cut(s) 652
SduI GDGCHC 1 cut(s) 1478
SfaNI GCATC 6 cut(s) 568, 919, 975, 976, 994, 1107
SinI GGWCC 1 cut(s) 376
SmiMI CAYNNNNRTG 1 cut(s) 428
SmlI CTYRAG 2 cut(s) 602, 1409
SmoI CTYRAG 2 cut(s) 602, 1409
SpeI ACTAGT 2 cut(s) 839, 1294
SphI GCATGC 1 cut(s) 1124
SsiI CCGC 7 cut(s) 87, 102, 105, 419, 575, 947, 1204
SspMI CTAG 6 cut(s) 341, 380, 444, 840, 1295, 1347
SstI GAGCTC 1 cut(s) 1478
StyD4I CCNGG 1 cut(s) 650
StyI CCWWGG 2 cut(s) 241, 318
TaaI ACNGT 5 cut(s) 121, 427, 478, 599, 1146
TaiI ACGT 2 cut(s) 21, 114
TaqI TCGA 6 cut(s) 114, 179, 352, 360, 403, 519
TaqII GACCGA 1 cut(s) 1436
TatI WGTACW 1 cut(s) 1139
TauI GCSGC 4 cut(s) 90, 107, 950, 1207
TfiI GAWTC 1 cut(s) 447
Tru1I TTAA 3 cut(s) 390, 615, 783
Tru9I TTAA 3 cut(s) 390, 615, 783
TscAI CASTG 3 cut(s) 151, 430, 1440
TseI GCWGC 3 cut(s) 90, 932, 935
TspDTI ATGAA 1 cut(s) 1496
TspGWI ACGGA 2 cut(s) 454, 923
TspRI CASTG 3 cut(s) 151, 430, 1440
Tth111I GACNNNGTC 1 cut(s) 348
VpaK11BI GGWCC 1 cut(s) 376
XagI CCTNNNNNAGG 2 cut(s) 544, 1363
XapI RAATTY 5 cut(s) 406, 515, 610, 958, 1465
XceI RCATGY 1 cut(s) 1124
XmiI GTMKAC 1 cut(s) 771
XmnI GAANNNNTTC 2 cut(s) 250, 1232
XspI CTAG 6 cut(s) 341, 380, 444, 840, 1295, 1347
ZraI GACGTC 1 cut(s) 112
Zsp2I ATGCAT 1 cut(s) 1122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.