pycom08g18730

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012017
Physical Location & Seq
Forward (+)
18812993 .. 18814504
1512 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g18730.1

Sequence Viewer

Length: 1512 bp
ATGGCATTATTCAGGTACGTCTCCTCGCAAAACCGAAAGTACCCACCTTTAGTCTTGTCTCTCTCTCGCTCTCTCTGCTCTGAAAGCGGCAGCTCAACCACCGCCGACGTCGAGACCGTTTATCGCATACTCAGTTGCCCCACTGGTTCCAAAAACTTGAAACAGGCATTGAATTCGAGTGGGGTTTTTCTCTCCAATGATTTGATTGATAAGGTTCTCAAACGGGTTCGGTTCAGCCATGGAAACCCTTCACAGGCATTGGAATTTTTCAATTACACCGGCAACAGAAGAGAGTTTTACCACACTCAGTTTTCCTTAGACACAATGCTTTACATTCTAGGACGAAGTCGAATGTTCGACAAAACTTGGGAGGTCTTAGTTGACACTAAGTATAAGGATCGAAATTTGATAACCCCGCGCACTGTAATGGTTGTGTTAGCTAGAATCGCTAAGGTTTGCTCCGTTAGGCAAACCGTTGAGTCTTTTAGGAAGTTCAAGAAGCTCGTGCCTGAATTCGATACAATGTGTTTCAATTCCTTGTTGAGGACTCTTTGTCAGGAGAAGAGTATGGCGGATGCTAGGAATGTGTATCACAGTCTCAAGCATAATTTTAAGCCGAATTTGCAAACTTTCAATATATTGCTGTCCGGGTGGAAGACATCCGAGGAAGCTGAGGGGTTCTTTGAGGAGATGAGGGAGATGGGTGTGAAACCTGATGTCGTTACCTACAATTGTTTGGTTGATGTTTATTGTAAGAATAGAGAAGTAGACAAGGCGTTTAAGATTGTAGAGGAAATGCGAGAACGGAATATATCACCTGATGTGATCACCTATACTAGTATCATTGGTGGATTGGGGTTGGTTGGTCAGCCAGATAAAGCCAGAGATATATTGAAGGAAATGACGGAGTATGGGTGCTACCCAGATGCTGCTGCATACAATGCGGCCATTAGGAATTTTTGCATCGCGAAGAGGCTTGGCGATGCTTATGGGTTGATGGATGCAATGGCGAGTAAGGGTTTGAGTCCGAATGCAACTACTTACAATTTGTTCTTCAGGGTTTTCTTCTGGTCAAATGACTTACAAAGCTCTTGGAGTTTGTATGGGAGAATGATGCATGCTGGGTGCTTACCAAGTACACAGTCTTGTATGTTCCTGATCAGGTTGTTTCGGAGACAGGAAAAGGTGGATATGGCATTGCGGCTGTGGAATGATATGATTGAGAAGGGTTTCGGTTCTTATATATTGGTATCTGATGTGCTGTTTGATTTGCTTTGTGATTTGGGAAAACTAGTGGAAGCAGAGAGGTGCTTCTTGCAGATGGTAGAAAAGGGGCAAAAGCCTAGTAGCGTTTCCTTCAGAAGGATCAAGGTGCTTATGGAACTGGCAAACAAGCATGAGGCCCTCAAGAACTTGACCGAGAAAATGGCAGTGTTTGGTGCTTCAATCCATCTTCCTGAGAATTCAAAGAGCTCAATGAATACTTCGCGTTTACAATCACATCCAATATAA

Protein Analysis

504

Amino Acids

57.57

Weight (kDa)

9.15

Isoelectric Point (pI)

41.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 173 - 217 2.3e-07 PPR repeat family
PPR_2 PF13041 222 - 248 1.6e-06 PPR repeat family
PPR_long PF17177 224 - 279 6.5e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 228 - 284 1.4e-14 Pentatricopeptide repeat domain
TPR_24 PF23276 231 - 318 1.7e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 234 - 267 2.6e-14 PPR repeat
PPR_2 PF13041 238 - 285 2.9e-19 PPR repeat family
PPR PF01535 241 - 271 5e-09 PPR repeat
PPR_long PF17177 259 - 354 5.6e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 261 - 318 3.6e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 269 - 302 1e-06 PPR repeat
PPR PF01535 276 - 306 4.9e-06 PPR repeat
PPR_2 PF13041 308 - 355 4.4e-09 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012017)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02420
fragaria_vesca FvH4_5g32610 FvH4_5g32610 FvH4_5g32610
malus_domestica MD08G1216300.v1.1
prunus_persica Prupe.1G549200_v2.0.a1
pyrus_communis pycom08g18730
rosa_chinensis RchiOBHm_Chr7g0230241
rosa_laevigata RLG00000001507
rosa_multiflora Rmu_sc0002822.1_g000019 Rmu_sc0008506.1_g000024
rosa_roxburghii Rroxscaffold_3G00230280
rosa_rugosa Rorug07G0260000
rosa_samantha Rh7AG409400 Rh7BG389300 Rh7CG428000 Rh7DG407000
rosa_wichuraiana Rw0G018590 Rw0G022620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 111
AccI GTMKAC 1 cut(s) 768
AccII CGCG 3 cut(s) 418, 968, 1489
AciI CCGC 6 cut(s) 87, 102, 416, 572, 944, 1201
AclWI GGATC 2 cut(s) 405, 1373
AcoI YGGCCR 1 cut(s) 945
AcsI RAATTY 7 cut(s) 172, 263, 403, 512, 619, 955, 1462
AcuI CTGAAG 2 cut(s) 1039, 1342
AcyI GRCGYC 1 cut(s) 108
AfaI GTAC 3 cut(s) 17, 41, 1138
AfiI CCNNNNNNNGG 3 cut(s) 253, 543, 1362
AgsI TTSAA 9 cut(s) 160, 172, 271, 496, 532, 634, 895, 1446, 1467
AhdI GACNNNNNGTC 1 cut(s) 552
AhlI ACTAGT 2 cut(s) 836, 1291
AluBI AGCT 6 cut(s) 93, 440, 502, 671, 1089, 1473
AluI AGCT 6 cut(s) 93, 440, 502, 671, 1089, 1473
Alw21I GWGCWC 1 cut(s) 1475
Alw26I GTCTC 5 cut(s) 25, 63, 107, 602, 1168
AlwI GGATC 2 cut(s) 405, 1373
AlwNI CAGNNNCTG 1 cut(s) 929
AoxI GGCC 2 cut(s) 945, 1401
ApeKI GCWGC 3 cut(s) 90, 929, 932
ApoI RAATTY 7 cut(s) 172, 263, 403, 512, 619, 955, 1462
Asp700I GAANNNNTTC 2 cut(s) 247, 1229
AspLEI GCGC 1 cut(s) 420
AspS9I GGNCC 1 cut(s) 1402
AsuC2I CCSGG 1 cut(s) 649
AsuHPI GGTGA 2 cut(s) 807, 820
BanII GRGCYC 1 cut(s) 1475
BauI CACGAG 1 cut(s) 503
BbsI GAAGAC 1 cut(s) 662
Bbv12I GWGCWC 1 cut(s) 1475
BbvCI CCTCAGC 1 cut(s) 672
BbvI GCAGC 3 cut(s) 102, 916, 919
BccI CCATC 4 cut(s) 694, 991, 1315, 1458
BcgI CGANNNNNNTGC 2 cut(s) 156, 190
BclI TGATCA 2 cut(s) 825, 1158
BcnI CCSGG 1 cut(s) 649
BcoDI GTCTC 5 cut(s) 25, 63, 107, 602, 1168
BcuI ACTAGT 2 cut(s) 836, 1291
BfaI CTAG 6 cut(s) 338, 441, 579, 837, 1292, 1344
BisI GCNGC 6 cut(s) 88, 91, 930, 933, 945, 1202
BlsI GCNGC 6 cut(s) 89, 92, 931, 934, 946, 1203
Bme1390I CCNGG 1 cut(s) 649
BmeRI GACNNNNNGTC 1 cut(s) 552
BmgT120I GGNCC 1 cut(s) 1402
BmiI GGNNCC 1 cut(s) 148
BmrFI CCNGG 1 cut(s) 649
BmsI GCATC 6 cut(s) 565, 916, 972, 973, 991, 1104
BpiI GAAGAC 1 cut(s) 662
Bpu10I CCTNAGC 2 cut(s) 450, 672
BpuEI CTTGAG 2 cut(s) 584, 1391
BpuMI CCSGG 1 cut(s) 649
BsaHI GRCGYC 1 cut(s) 108
BsaI GGTCTC 1 cut(s) 107
BsaJI CCNNGG 2 cut(s) 238, 663
BsaXI ACNNNNNCTCC 2 cut(s) 899, 929
Bsc4I CCNNNNNNNGG 3 cut(s) 253, 543, 1362
Bse118I RCCGGY 1 cut(s) 278
Bse1I ACTGG 2 cut(s) 148, 1389
Bse3DI GCAATG 2 cut(s) 1011, 1196
BseDI CCNNGG 2 cut(s) 238, 663
BseGI GGATG 4 cut(s) 580, 659, 1006, 1501
BseLI CCNNNNNNNGG 3 cut(s) 253, 543, 1362
BseMI GCAATG 2 cut(s) 1011, 1196
BseMII CTCAG 4 cut(s) 145, 320, 663, 1449
BseNI ACTGG 2 cut(s) 148, 1389
BseRI GAGGAG 2 cut(s) 13, 701
BseXI GCAGC 3 cut(s) 102, 916, 919
BseYI CCCAGC 1 cut(s) 1121
Bsh1236I CGCG 3 cut(s) 418, 968, 1489
BshFI GGCC 2 cut(s) 947, 1403
BsiHKAI GWGCWC 1 cut(s) 1475
BsiSI CCGG 2 cut(s) 279, 648
BslI CCNNNNNNNGG 3 cut(s) 253, 543, 1362
BsmAI GTCTC 5 cut(s) 25, 63, 107, 602, 1168
BsmBI CGTCTC 1 cut(s) 25
BsmI GAATGC 1 cut(s) 1036
BsnI GGCC 2 cut(s) 947, 1403
Bso31I GGTCTC 1 cut(s) 107
Bsp1286I GDGCHC 1 cut(s) 1475
Bsp143I GATC 4 cut(s) 397, 825, 1158, 1365
Bsp19I CCATGG 1 cut(s) 238
Bsp68I TCGCGA 1 cut(s) 968
BspACI CCGC 6 cut(s) 87, 102, 416, 572, 944, 1201
BspANI GGCC 2 cut(s) 947, 1403
BspCNI CTCAG 4 cut(s) 144, 319, 664, 1450
BspFNI CGCG 3 cut(s) 418, 968, 1489
BspLI GGNNCC 1 cut(s) 148
BspPI GGATC 2 cut(s) 405, 1373
BspTNI GGTCTC 1 cut(s) 107
BsrDI GCAATG 2 cut(s) 1011, 1196
BsrFI RCCGGY 1 cut(s) 278
BsrI ACTGG 2 cut(s) 148, 1389
BssAI RCCGGY 1 cut(s) 278
BssECI CCNNGG 2 cut(s) 238, 663
BssMI GATC 4 cut(s) 397, 825, 1158, 1365
BssNI GRCGYC 1 cut(s) 108
BssSI CACGAG 1 cut(s) 503
BssT1I CCWWGG 1 cut(s) 238
Bst2BI CACGAG 1 cut(s) 503
Bst4CI ACNGT 5 cut(s) 118, 424, 475, 596, 1143
Bst6I CTCTTC 3 cut(s) 283, 557, 965
BstACI GRCGYC 1 cut(s) 108
BstAPI GCANNNNNTGC 1 cut(s) 941
BstC8I GCNNGC 1 cut(s) 1119
BstDEI CTNAG 8 cut(s) 131, 306, 316, 376, 387, 450, 672, 1458
BstDSI CCRYGG 1 cut(s) 238
BstENI CCTNNNNNAGG 2 cut(s) 541, 1360
BstF5I GGATG 4 cut(s) 580, 659, 1006, 1501
BstFNI CGCG 3 cut(s) 418, 968, 1489
BstHHI GCGC 1 cut(s) 420
BstKTI GATC 4 cut(s) 400, 828, 1161, 1368
BstMAI GTCTC 5 cut(s) 25, 63, 107, 602, 1168
BstMBI GATC 4 cut(s) 397, 825, 1158, 1365
BstMWI GCNNNNNNNGC 5 cut(s) 75, 84, 446, 622, 941
BstNSI RCATGY 1 cut(s) 1121
BstSCI CCNGG 1 cut(s) 647
BstUI CGCG 3 cut(s) 418, 968, 1489
BstV1I GCAGC 3 cut(s) 102, 916, 919
BstV2I GAAGAC 1 cut(s) 662
BsuRI GGCC 2 cut(s) 947, 1403
BtgI CCRYGG 1 cut(s) 238
BtgZI GCGATG 2 cut(s) 949, 996
BtsCI GGATG 4 cut(s) 580, 659, 1006, 1501
BtsI GCAGTG 1 cut(s) 1437
BtsIMutI CAGTG 3 cut(s) 141, 420, 1437
BtuMI TCGCGA 1 cut(s) 968
Cac8I GCNNGC 1 cut(s) 1119
CaiI CAGNNNCTG 1 cut(s) 929
CfoI GCGC 1 cut(s) 420
Cfr10I RCCGGY 1 cut(s) 278
Cfr13I GGNCC 1 cut(s) 1402
Csp6I GTAC 3 cut(s) 16, 40, 1137
CviAII CATG 3 cut(s) 239, 1118, 1397
CviQI GTAC 3 cut(s) 16, 40, 1137
DdeI CTNAG 8 cut(s) 131, 306, 316, 376, 387, 450, 672, 1458
DpnI GATC 4 cut(s) 399, 827, 1160, 1367
DpnII GATC 4 cut(s) 397, 825, 1158, 1365
DriI GACNNNNNGTC 1 cut(s) 552
EaeI YGGCCR 1 cut(s) 945
Eam1104I CTCTTC 3 cut(s) 283, 557, 965
Eam1105I GACNNNNNGTC 1 cut(s) 552
EarI CTCTTC 3 cut(s) 283, 557, 965
EciI GGCGGA 1 cut(s) 587
Ecl136II GAGCTC 1 cut(s) 1473
Eco130I CCWWGG 1 cut(s) 238
Eco24I GRGCYC 1 cut(s) 1475
Eco31I GGTCTC 1 cut(s) 107
Eco53kI GAGCTC 1 cut(s) 1473
Eco57I CTGAAG 2 cut(s) 1039, 1342
EcoICRI GAGCTC 1 cut(s) 1473
EcoNI CCTNNNNNAGG 2 cut(s) 541, 1360
EcoO109I RGGNCCY 1 cut(s) 1402
EcoRI GAATTC 3 cut(s) 172, 512, 1462
EcoT14I CCWWGG 1 cut(s) 238
EcoT22I ATGCAT 1 cut(s) 1119
EcoT38I GRGCYC 1 cut(s) 1475
ErhI CCWWGG 1 cut(s) 238
Esp3I CGTCTC 1 cut(s) 25
FaeI CATG 3 cut(s) 242, 1121, 1400
FatI CATG 3 cut(s) 238, 1117, 1396
FauI CCCGC 1 cut(s) 423
FbaI TGATCA 2 cut(s) 825, 1158
FblI GTMKAC 1 cut(s) 768
Fnu4HI GCNGC 6 cut(s) 88, 91, 930, 933, 945, 1202
FokI GGATG 4 cut(s) 587, 646, 1013, 1488
FriOI GRGCYC 1 cut(s) 1475
Fsp4HI GCNGC 6 cut(s) 88, 91, 930, 933, 945, 1202
FspBI CTAG 6 cut(s) 338, 441, 579, 837, 1292, 1344
GlaI GCGC 1 cut(s) 419
GluI GCNGC 6 cut(s) 88, 91, 930, 933, 945, 1202
GsaI CCCAGC 1 cut(s) 1125
HaeIII GGCC 2 cut(s) 947, 1403
HapII CCGG 2 cut(s) 279, 648
HhaI GCGC 1 cut(s) 420
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 3 cut(s) 242, 1121, 1400
Hin6I GCGC 1 cut(s) 418
HinP1I GCGC 1 cut(s) 418
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HinfI GANTC 4 cut(s) 444, 479, 547, 1024
HpaII CCGG 2 cut(s) 279, 648
HphI GGTGA 2 cut(s) 807, 820
Hpy166II GTNNAC 4 cut(s) 382, 769, 1139, 1493
Hpy188I TCNGA 6 cut(s) 82, 664, 1029, 1173, 1255, 1361
Hpy188III TCNNGA 7 cut(s) 112, 496, 557, 967, 1156, 1408, 1457
Hpy8I GTNNAC 4 cut(s) 382, 769, 1139, 1493
Hpy99I CGWCG 2 cut(s) 110, 113
HpyAV CCTTC 5 cut(s) 258, 889, 1219, 1356, 1366
HpyCH4III ACNGT 5 cut(s) 118, 424, 475, 596, 1143
HpyCH4IV ACGT 2 cut(s) 18, 108
HpyCH4V TGCA 7 cut(s) 625, 935, 963, 1004, 1034, 1117, 1318
HpyF10VI GCNNNNNNNGC 5 cut(s) 75, 84, 446, 622, 941
HpyF3I CTNAG 8 cut(s) 131, 306, 316, 376, 387, 450, 672, 1458
HpySE526I ACGT 2 cut(s) 18, 108
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 3 cut(s) 242, 1121, 1400
HspAI GCGC 1 cut(s) 418
Ksp22I TGATCA 2 cut(s) 825, 1158
Kzo9I GATC 4 cut(s) 397, 825, 1158, 1365
LmnI GCTCC 1 cut(s) 464
Lsp1109I GCAGC 3 cut(s) 102, 916, 919
LweI GCATC 6 cut(s) 565, 916, 972, 973, 991, 1104
MaeI CTAG 6 cut(s) 338, 441, 579, 837, 1292, 1344
MaeII ACGT 2 cut(s) 18, 108
MaeIII GTNAC 1 cut(s) 721
MalI GATC 4 cut(s) 399, 827, 1160, 1367
MboI GATC 4 cut(s) 397, 825, 1158, 1365
MboII GAAGA 7 cut(s) 300, 574, 667, 982, 1045, 1057, 1445
MfeI CAATTG 1 cut(s) 730
MhlI GDGCHC 1 cut(s) 1475
MlyI GAGTC 3 cut(s) 488, 541, 1033
Mph1103I ATGCAT 1 cut(s) 1119
MroXI GAANNNNTTC 2 cut(s) 247, 1229
MseI TTAA 2 cut(s) 612, 780
MslI CAYNNNNRTG 1 cut(s) 425
MspI CCGG 2 cut(s) 279, 648
MspR9I CCNGG 1 cut(s) 649
MunI CAATTG 1 cut(s) 730
Mva1269I GAATGC 1 cut(s) 1036
MvnI CGCG 3 cut(s) 418, 968, 1489
MwoI GCNNNNNNNGC 5 cut(s) 75, 84, 446, 622, 941
NciI CCSGG 1 cut(s) 649
NcoI CCATGG 1 cut(s) 238
NdeII GATC 4 cut(s) 397, 825, 1158, 1365
NlaIII CATG 3 cut(s) 242, 1121, 1400
NlaIV GGNNCC 1 cut(s) 148
NruI TCGCGA 1 cut(s) 968
NsiI ATGCAT 1 cut(s) 1119
NspI RCATGY 1 cut(s) 1121
PaeI GCATGC 1 cut(s) 1121
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PctI GAATGC 1 cut(s) 1036
PdmI GAANNNNTTC 2 cut(s) 247, 1229
PfeI GAWTC 1 cut(s) 444
PflFI GACNNNGTC 1 cut(s) 345
PkrI GCNGC 6 cut(s) 89, 92, 931, 934, 946, 1203
PleI GAGTC 3 cut(s) 487, 541, 1032
PpsI GAGTC 3 cut(s) 487, 541, 1032
Psp124BI GAGCTC 1 cut(s) 1475
PspFI CCCAGC 1 cut(s) 1121
PspN4I GGNNCC 1 cut(s) 148
PspPI GGNCC 1 cut(s) 1402
PstNI CAGNNNCTG 1 cut(s) 929
PsyI GACNNNGTC 1 cut(s) 345
RruI TCGCGA 1 cut(s) 968
RsaI GTAC 3 cut(s) 17, 41, 1138
RsaNI GTAC 3 cut(s) 16, 40, 1137
RseI CAYNNNNRTG 1 cut(s) 425
SacI GAGCTC 1 cut(s) 1475
SaqAI TTAA 2 cut(s) 612, 780
SatI GCNGC 6 cut(s) 88, 91, 930, 933, 945, 1202
Sau3AI GATC 4 cut(s) 397, 825, 1158, 1365
Sau96I GGNCC 1 cut(s) 1402
SchI GAGTC 3 cut(s) 488, 541, 1033
ScrFI CCNGG 1 cut(s) 649
SduI GDGCHC 1 cut(s) 1475
SfaNI GCATC 6 cut(s) 565, 916, 972, 973, 991, 1104
SmiMI CAYNNNNRTG 1 cut(s) 425
SmlI CTYRAG 2 cut(s) 599, 1406
SmoI CTYRAG 2 cut(s) 599, 1406
SpeI ACTAGT 2 cut(s) 836, 1291
SphI GCATGC 1 cut(s) 1121
SsiI CCGC 6 cut(s) 87, 102, 416, 572, 944, 1201
SspMI CTAG 6 cut(s) 338, 441, 579, 837, 1292, 1344
SstI GAGCTC 1 cut(s) 1475
StyD4I CCNGG 1 cut(s) 647
StyI CCWWGG 1 cut(s) 238
TaaI ACNGT 5 cut(s) 118, 424, 475, 596, 1143
TaiI ACGT 2 cut(s) 21, 111
TaqI TCGA 6 cut(s) 111, 176, 349, 357, 400, 516
TaqII GACCGA 1 cut(s) 1433
TatI WGTACW 1 cut(s) 1136
TauI GCSGC 3 cut(s) 90, 947, 1204
TfiI GAWTC 1 cut(s) 444
Tru1I TTAA 2 cut(s) 612, 780
Tru9I TTAA 2 cut(s) 612, 780
TscAI CASTG 3 cut(s) 148, 427, 1437
TseI GCWGC 3 cut(s) 90, 929, 932
TspDTI ATGAA 1 cut(s) 1493
TspGWI ACGGA 3 cut(s) 451, 820, 920
TspRI CASTG 3 cut(s) 148, 427, 1437
Tth111I GACNNNGTC 1 cut(s) 345
XagI CCTNNNNNAGG 2 cut(s) 541, 1360
XapI RAATTY 7 cut(s) 172, 263, 403, 512, 619, 955, 1462
XceI RCATGY 1 cut(s) 1121
XmiI GTMKAC 1 cut(s) 768
XmnI GAANNNNTTC 2 cut(s) 247, 1229
XspI CTAG 6 cut(s) 338, 441, 579, 837, 1292, 1344
ZraI GACGTC 1 cut(s) 109
Zsp2I ATGCAT 1 cut(s) 1119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.