RLG00000001507

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0012017
Physical Location & Seq
Forward (+)
16602050 .. 16606812
4763 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001507

Sequence Viewer

Length: 1833 bp
ATGATGATGGCACTCAAAGCTAAACCCATCTCATCCCCATCACCATTTTTCAGGTACTCGCCGCAAAACCCAATTTTCTTTCTCCCTCGATCACTCTCTTCCCAAACCCCAATCGACAGTGACGTCGACACTGTCTACCGCATAGTCAGTAGCTCCACCCACTCCAAAAACTTGAAGCAAAACTTGAAATCGAGTGGTGTTTTCCTCTCAAACGATTTGATAGATGAGGTTCTGAAGCGTGCTAGGTTCAGCCATGGAAACCCTTTACAGGCTCTCGAGTTTTTCAATTACACTGGGAATAGGAGAGGGTTTTATCACACCACATTTGCACTGGACACAATGCTTTATATGTTGGGAAGAAGTCGAATGTTCGAAAAGATGTGGGAGGTTTTGGTTGAGATCAAGAATAAGGATAGGAATTTGATTACTCCGCGTACTGTGATGGTAGTGTTAGCTAGGATTGCTAAGGTTTGTTCTGTTAGGGAGACAGTGGAATGTTTTAGAAAGTTCAAGAAGCTTGTGCCCGAATTCGACACAGCTTGCTTCAATGCGTTGTTGAGGACTCTTTGTCAAGAGAAGAGTATGACTGATGCTAGGAATGTGTATCATAAGCTGAAGCAGAGCTTTAGGCCGAATTTGCAGACCTTTAATATATTGTTATCGGGTTGGAAGTCATCGGAGGAAGCTGAGGGGTTTTTCGAGGAGATGAGGGAGATGGGTCTGAAACCTGATATTGTTTCGTATAATTGTTTGATTGATGTTTATAGTAAGAATAGAGAAATGGCGAAAGCGTTTAAGGTGATGGAAAAAATGCGTGACGAGGATATATCACCTGATAAGATCACGTATACTTGTGTCATTGGGGGATTGGGGTTGGTTGGTCAGCCTGATAAAGCCAAAGATGTGTTGAAGGAAATGAAGGAGCTTGGGTGCTACCCGGATGTGGCAGCATACAATGCTGCCATTAGGAATTTTTGCATTGCAAAGAGGCTTGGTGATGCTAATGGGTTGATGGAGGAGATGATAAGTAATGGTTTGAGTCCAAATGCAACTACTTACAATTTATTCTTTAGGGTTTTCTTCTGGTCAAGTGACTTGCAGAACTCTTGGAGCTTGTATGGGAGAATGATGCATACTGGGTGTCTGCCAAATACACAGTCGTGCATGTTCCTGATCAGGTTGTTTAGGAGGCTGGAAAAGGTGGATATGGCATTGCAGCTGTGGAATGATATGGTTGAGAGGGGTTTTGGGTCTTATATATTGGTATCTGATGTGCTGTTTGATTTGCTTTGTGATATGGGTAAACTAACAGAAGCAGAAACGTGCTTCTTGCAGATGGTGGAGAAGGGGCATAAGCCAAGTAACGTTTCCTTTAGAAGGATCAAGGTGCTTATGGAACTGGCAAACAAGCATGAGGCCCTCAAGAACTTGACAGAGAAAATGGCACTTTTTGGCTCTTCAATCCATCTTCCTGACAACAAGCATCCAGTTAGAGTCAGAATTACAGTAAGGAAATATGTATCTGTGAGGCAAAATCACAACAGAGCCATTTTCACAACCTTTGTCCCATCAGATGATAGCAAGGACTTGGAAGTTTGTGCACTTGCAGTTATTCCTGAGCATGTGCAGCAGGGTGGAGGGAGCTTCAGCTCAAAGTGTTGCATGATTGGAAGACCAATAAAAATGGTTGCAAGGCACGTTCCTTTGTTAGAAACACCTGTTGGTCCTGTTTTCGAGAGCAATGCCATTGCACACTATGTTACTAGCTTGAAGGCAAATAATACTCTGTTTGGTTCTTCCTTATTTGAACATCCCTTGTGGAGCAATGGATAG

Protein Analysis

611

Amino Acids

69.58

Weight (kDa)

9.11

Isoelectric Point (pI)

44.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 178 - 222 7.9e-07 PPR repeat family
PPR_3 PF13812 233 - 289 6.4e-11 Pentatricopeptide repeat domain
PPR_long PF17177 233 - 359 4.1e-10 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 236 - 336 2.7e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 239 - 272 9.1e-09 PPR repeat
PPR_2 PF13041 243 - 290 4.9e-16 PPR repeat family
PPR PF01535 246 - 275 1.3e-06 PPR repeat
PPR_3 PF13812 266 - 322 1.2e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 275 - 307 8.7e-06 PPR repeat
PPR PF01535 281 - 311 9.8e-07 PPR repeat
PPR_2 PF13041 287 - 326 1.7e-06 PPR repeat family
PPR_3 PF13812 302 - 359 9.4e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 313 - 360 4.9e-10 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012017)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02420
fragaria_vesca FvH4_5g32610 FvH4_5g32610 FvH4_5g32610
malus_domestica MD08G1216300.v1.1
prunus_persica Prupe.1G549200_v2.0.a1
pyrus_communis pycom08g18730
rosa_chinensis RchiOBHm_Chr7g0230241
rosa_laevigata RLG00000001507
rosa_multiflora Rmu_sc0002822.1_g000019 Rmu_sc0008506.1_g000024
rosa_roxburghii Rroxscaffold_3G00230280
rosa_rugosa Rorug07G0260000
rosa_samantha Rh7AG409400 Rh7BG389300 Rh7CG428000 Rh7DG407000
rosa_wichuraiana Rw0G018590 Rw0G022620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 122
AatII GACGTC 1 cut(s) 126
AccI GTMKAC 3 cut(s) 126, 135, 848
AccII CGCG 1 cut(s) 433
AciI CCGC 3 cut(s) 62, 139, 431
AclI AACGTT 1 cut(s) 1365
AclWI GGATC 1 cut(s) 1388
AcsI RAATTY 4 cut(s) 418, 527, 634, 970
AcuI CTGAAG 3 cut(s) 254, 635, 1630
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 2 cut(s) 56, 436
AfiI CCNNNNNNNGG 3 cut(s) 268, 943, 1377
AgsI TTSAA 9 cut(s) 175, 187, 286, 511, 547, 910, 1461, 1771, 1808
AhdI GACNNNNNGTC 1 cut(s) 567
AjuI GAANNNNNNNTTGG 4 cut(s) 1351, 1383, 1704, 1736
AleI CACNNNNGTG 1 cut(s) 1159
Alw21I GWGCWC 1 cut(s) 1603
Alw26I GTCTC 1 cut(s) 479
Alw44I GTGCAC 1 cut(s) 1599
AlwI GGATC 1 cut(s) 1388
Ama87I CYCGRG 1 cut(s) 275
AoxI GGCC 2 cut(s) 629, 1416
ApaLI GTGCAC 1 cut(s) 1599
ApeKI GCWGC 4 cut(s) 947, 959, 1216, 1627
ApoI RAATTY 4 cut(s) 418, 527, 634, 970
ArsI GACNNNNNNTTYG 2 cut(s) 1142, 1174
AspS9I GGNCC 2 cut(s) 1417, 1724
AsuC2I CCSGG 1 cut(s) 938
AsuHPI GGTGA 4 cut(s) 33, 811, 822, 1007
AsuII TTCGAA 1 cut(s) 372
AvaI CYCGRG 1 cut(s) 275
AvaII GGWCC 1 cut(s) 1724
BaeGI GKGCMC 2 cut(s) 525, 1603
BbsI GAAGAC 1 cut(s) 1678
Bbv12I GWGCWC 1 cut(s) 1603
BbvCI CCTCAGC 1 cut(s) 687
BbvI GCAGC 4 cut(s) 946, 959, 1228, 1639
BccI CCATC 9 cut(s) 35, 46, 436, 709, 796, 1006, 1330, 1473, 1576
BcgI CGANNNNNNTGC 2 cut(s) 1724, 1758
BclI TGATCA 1 cut(s) 1173
BcnI CCSGG 1 cut(s) 938
BcoDI GTCTC 1 cut(s) 479
BfaI CTAG 4 cut(s) 243, 456, 594, 1764
BisI GCNGC 5 cut(s) 62, 948, 960, 1217, 1628
BlsI GCNGC 5 cut(s) 63, 949, 961, 1218, 1629
Bme1390I CCNGG 1 cut(s) 938
Bme18I GGWCC 1 cut(s) 1724
BmeRI GACNNNNNGTC 1 cut(s) 567
BmeT110I CYCGRG 1 cut(s) 275
BmgT120I GGNCC 2 cut(s) 1417, 1724
BmrFI CCNGG 1 cut(s) 938
BmrI ACTGGG 2 cut(s) 303, 1146
BmsI GCATC 4 cut(s) 580, 988, 1119, 1492
BmuI ACTGGG 2 cut(s) 303, 1146
BpiI GAAGAC 1 cut(s) 1678
Bpu10I CCTNAGC 3 cut(s) 465, 687, 1617
Bpu14I TTCGAA 1 cut(s) 372
BpuEI CTTGAG 1 cut(s) 1406
BpuMI CCSGG 1 cut(s) 938
BsaAI YACGTR 1 cut(s) 846
BsaHI GRCGYC 1 cut(s) 123
BsaJI CCNNGG 1 cut(s) 253
BsaXI ACNNNNNCTCC 2 cut(s) 914, 944
Bsc4I CCNNNNNNNGG 3 cut(s) 268, 943, 1377
Bse1I ACTGG 5 cut(s) 298, 336, 1141, 1404, 1487
Bse3DI GCAATG 5 cut(s) 978, 1211, 1746, 1747, 1831
BseDI CCNNGG 1 cut(s) 253
BseGI GGATG 4 cut(s) 32, 946, 1483, 1810
BseLI CCNNNNNNNGG 3 cut(s) 268, 943, 1377
BseMI GCAATG 5 cut(s) 978, 1211, 1746, 1747, 1831
BseMII CTCAG 2 cut(s) 678, 1608
BseNI ACTGG 5 cut(s) 298, 336, 1141, 1404, 1487
BseRI GAGGAG 2 cut(s) 716, 1031
BseSI GKGCMC 2 cut(s) 525, 1603
BseXI GCAGC 4 cut(s) 946, 959, 1228, 1639
BsgI GTGCAG 1 cut(s) 1646
Bsh1236I CGCG 1 cut(s) 433
BshFI GGCC 2 cut(s) 631, 1418
BsiHKAI GWGCWC 1 cut(s) 1603
BsiHKCI CYCGRG 1 cut(s) 275
BsiSI CCGG 1 cut(s) 938
BslFI GGGAC 1 cut(s) 1550
BslI CCNNNNNNNGG 3 cut(s) 268, 943, 1377
BsmAI GTCTC 1 cut(s) 479
BsmFI GGGAC 1 cut(s) 1550
BsnI GGCC 2 cut(s) 631, 1418
BsoBI CYCGRG 1 cut(s) 275
Bsp119I TTCGAA 1 cut(s) 372
Bsp1286I GDGCHC 2 cut(s) 525, 1603
Bsp143I GATC 5 cut(s) 89, 399, 840, 1173, 1380
Bsp19I CCATGG 1 cut(s) 253
BspACI CCGC 3 cut(s) 62, 139, 431
BspANI GGCC 2 cut(s) 631, 1418
BspCNI CTCAG 2 cut(s) 679, 1609
BspFNI CGCG 1 cut(s) 433
BspPI GGATC 1 cut(s) 1388
BspQI GCTCTTC 1 cut(s) 1462
BspT104I TTCGAA 1 cut(s) 372
BsrDI GCAATG 5 cut(s) 978, 1211, 1746, 1747, 1831
BsrI ACTGG 5 cut(s) 298, 336, 1141, 1404, 1487
BssECI CCNNGG 1 cut(s) 253
BssMI GATC 5 cut(s) 89, 399, 840, 1173, 1380
BssNAI GTATAC 1 cut(s) 849
BssNI GRCGYC 1 cut(s) 123
BssT1I CCWWGG 1 cut(s) 253
Bst1107I GTATAC 1 cut(s) 849
Bst4CI ACNGT 6 cut(s) 119, 133, 439, 490, 1158, 1507
Bst6I CTCTTC 3 cut(s) 103, 572, 1462
BstACI GRCGYC 1 cut(s) 123
BstAPI GCANNNNNTGC 1 cut(s) 956
BstBAI YACGTR 1 cut(s) 846
BstBI TTCGAA 1 cut(s) 372
BstC8I GCNNGC 2 cut(s) 240, 541
BstDEI CTNAG 3 cut(s) 465, 687, 1617
BstDSI CCRYGG 1 cut(s) 253
BstENI CCTNNNNNAGG 1 cut(s) 1375
BstF5I GGATG 4 cut(s) 32, 946, 1483, 1810
BstFNI CGCG 1 cut(s) 433
BstKTI GATC 5 cut(s) 92, 402, 843, 1176, 1383
BstMAI GTCTC 1 cut(s) 479
BstMBI GATC 5 cut(s) 89, 399, 840, 1173, 1380
BstMWI GCNNNNNNNGC 5 cut(s) 17, 461, 637, 956, 1627
BstNSI RCATGY 2 cut(s) 1168, 1625
BstSCI CCNGG 1 cut(s) 936
BstSLI GKGCMC 2 cut(s) 525, 1603
BstUI CGCG 1 cut(s) 433
BstV1I GCAGC 4 cut(s) 946, 959, 1228, 1639
BstV2I GAAGAC 1 cut(s) 1678
BstZ17I GTATAC 1 cut(s) 849
BsuRI GGCC 2 cut(s) 631, 1418
BtgI CCRYGG 1 cut(s) 253
BtsCI GGATG 4 cut(s) 32, 946, 1483, 1810
BtsIMutI CAGTG 5 cut(s) 124, 129, 291, 329, 495
Cac8I GCNNGC 2 cut(s) 240, 541
Cfr13I GGNCC 2 cut(s) 1417, 1724
Csp6I GTAC 2 cut(s) 55, 435
CviAII CATG 5 cut(s) 254, 1165, 1412, 1622, 1663
CviQI GTAC 2 cut(s) 55, 435
DdeI CTNAG 3 cut(s) 465, 687, 1617
DpnI GATC 5 cut(s) 91, 401, 842, 1175, 1382
DpnII GATC 5 cut(s) 89, 399, 840, 1173, 1380
DrdI GACNNNNNNGTC 1 cut(s) 122
DriI GACNNNNNGTC 1 cut(s) 567
DseDI GACNNNNNNGTC 1 cut(s) 122
Eam1104I CTCTTC 3 cut(s) 103, 572, 1462
Eam1105I GACNNNNNGTC 1 cut(s) 567
EarI CTCTTC 3 cut(s) 103, 572, 1462
Eco130I CCWWGG 1 cut(s) 253
Eco47I GGWCC 1 cut(s) 1724
Eco57I CTGAAG 3 cut(s) 254, 635, 1630
Eco88I CYCGRG 1 cut(s) 275
EcoNI CCTNNNNNAGG 1 cut(s) 1375
EcoO109I RGGNCCY 1 cut(s) 1417
EcoRI GAATTC 1 cut(s) 527
EcoT14I CCWWGG 1 cut(s) 253
EcoT22I ATGCAT 1 cut(s) 1134
ErhI CCWWGG 1 cut(s) 253
FaeI CATG 5 cut(s) 257, 1168, 1415, 1625, 1666
FalI AAGNNNNNCTT 4 cut(s) 167, 199, 608, 640
FaqI GGGAC 1 cut(s) 1550
FatI CATG 5 cut(s) 253, 1164, 1411, 1621, 1662
FbaI TGATCA 1 cut(s) 1173
FblI GTMKAC 3 cut(s) 126, 135, 848
Fnu4HI GCNGC 5 cut(s) 62, 948, 960, 1217, 1628
FokI GGATG 4 cut(s) 19, 953, 1470, 1797
Fsp4HI GCNGC 5 cut(s) 62, 948, 960, 1217, 1628
FspBI CTAG 4 cut(s) 243, 456, 594, 1764
GluI GCNGC 5 cut(s) 62, 948, 960, 1217, 1628
HaeIII GGCC 2 cut(s) 631, 1418
HapII CCGG 1 cut(s) 938
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 5 cut(s) 257, 1168, 1415, 1625, 1666
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HindIII AAGCTT 1 cut(s) 515
HinfI GANTC 3 cut(s) 562, 1039, 1494
HpaII CCGG 1 cut(s) 938
HphI GGTGA 4 cut(s) 33, 811, 822, 1007
Hpy166II GTNNAC 5 cut(s) 127, 136, 849, 1304, 1601
Hpy188I TCNGA 6 cut(s) 234, 679, 723, 1270, 1499, 1573
Hpy188III TCNNGA 9 cut(s) 275, 403, 511, 572, 1171, 1423, 1472, 1616, 1735
Hpy8I GTNNAC 5 cut(s) 127, 136, 849, 1304, 1601
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 5 cut(s) 904, 913, 1339, 1371, 1765
HpyCH4III ACNGT 6 cut(s) 119, 133, 439, 490, 1158, 1507
HpyCH4IV ACGT 5 cut(s) 123, 845, 1322, 1365, 1698
HpyF10VI GCNNNNNNNGC 5 cut(s) 17, 461, 637, 956, 1627
HpyF3I CTNAG 3 cut(s) 465, 687, 1617
HpySE526I ACGT 5 cut(s) 123, 845, 1322, 1365, 1698
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 5 cut(s) 257, 1168, 1415, 1625, 1666
Ksp22I TGATCA 1 cut(s) 1173
Kzo9I GATC 5 cut(s) 89, 399, 840, 1173, 1380
LguI GCTCTTC 1 cut(s) 1462
LmnI GCTCC 5 cut(s) 158, 922, 1110, 1641, 1821
Lsp1109I GCAGC 4 cut(s) 946, 959, 1228, 1639
LweI GCATC 4 cut(s) 580, 988, 1119, 1492
MaeI CTAG 4 cut(s) 243, 456, 594, 1764
MaeII ACGT 5 cut(s) 123, 845, 1322, 1365, 1698
MaeIII GTNAC 5 cut(s) 119, 815, 1091, 1361, 1759
MalI GATC 5 cut(s) 91, 401, 842, 1175, 1382
MboI GATC 5 cut(s) 89, 399, 840, 1173, 1380
MboII GAAGA 8 cut(s) 90, 369, 589, 1072, 1449, 1460, 1683, 1788
MhlI GDGCHC 2 cut(s) 525, 1603
MluCI AATT 9 cut(s) 72, 286, 418, 527, 634, 745, 970, 1060, 1500
MlyI GAGTC 3 cut(s) 556, 1048, 1503
MmeI TCCRAC 1 cut(s) 647
Mph1103I ATGCAT 1 cut(s) 1134
MseI TTAA 2 cut(s) 648, 795
MslI CAYNNNNRTG 1 cut(s) 1159
MspA1I CMGCKG 1 cut(s) 1219
MspI CCGG 1 cut(s) 938
MspR9I CCNGG 1 cut(s) 938
MvnI CGCG 1 cut(s) 433
MwoI GCNNNNNNNGC 5 cut(s) 17, 461, 637, 956, 1627
NciI CCSGG 1 cut(s) 938
NcoI CCATGG 1 cut(s) 253
NdeII GATC 5 cut(s) 89, 399, 840, 1173, 1380
NlaIII CATG 5 cut(s) 257, 1168, 1415, 1625, 1666
NmuCI GTSAC 3 cut(s) 119, 815, 1091
NsiI ATGCAT 1 cut(s) 1134
NspI RCATGY 2 cut(s) 1168, 1625
NspV TTCGAA 1 cut(s) 372
OliI CACNNNNGTG 1 cut(s) 1159
PaeR7I CTCGAG 1 cut(s) 275
PciSI GCTCTTC 1 cut(s) 1462
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PflFI GACNNNGTC 1 cut(s) 131
PkrI GCNGC 5 cut(s) 63, 949, 961, 1218, 1629
PleI GAGTC 3 cut(s) 556, 1047, 1502
PpsI GAGTC 3 cut(s) 556, 1047, 1502
Ppu21I YACGTR 1 cut(s) 846
Psp1406I AACGTT 1 cut(s) 1365
PspPI GGNCC 2 cut(s) 1417, 1724
PsyI GACNNNGTC 1 cut(s) 131
PvuII CAGCTG 1 cut(s) 1219
RsaI GTAC 2 cut(s) 56, 436
RsaNI GTAC 2 cut(s) 55, 435
RseI CAYNNNNRTG 1 cut(s) 1159
SalI GTCGAC 1 cut(s) 125
SapI GCTCTTC 1 cut(s) 1462
SaqAI TTAA 2 cut(s) 648, 795
SatI GCNGC 5 cut(s) 62, 948, 960, 1217, 1628
Sau3AI GATC 5 cut(s) 89, 399, 840, 1173, 1380
Sau96I GGNCC 2 cut(s) 1417, 1724
SchI GAGTC 3 cut(s) 556, 1048, 1503
ScrFI CCNGG 1 cut(s) 938
SduI GDGCHC 2 cut(s) 525, 1603
SfaNI GCATC 4 cut(s) 580, 988, 1119, 1492
Sfr274I CTCGAG 1 cut(s) 275
SfuI TTCGAA 1 cut(s) 372
SinI GGWCC 1 cut(s) 1724
SlaI CTCGAG 1 cut(s) 275
SmiMI CAYNNNNRTG 1 cut(s) 1159
SmlI CTYRAG 2 cut(s) 275, 1421
SmoI CTYRAG 2 cut(s) 275, 1421
Sse9I AATT 9 cut(s) 72, 286, 418, 527, 634, 745, 970, 1060, 1500
SsiI CCGC 3 cut(s) 62, 139, 431
SspMI CTAG 4 cut(s) 243, 456, 594, 1764
StyD4I CCNGG 1 cut(s) 936
StyI CCWWGG 1 cut(s) 253
TaaI ACNGT 6 cut(s) 119, 133, 439, 490, 1158, 1507
TaiI ACGT 5 cut(s) 126, 848, 1325, 1368, 1701
TasI AATT 9 cut(s) 72, 286, 418, 527, 634, 745, 970, 1060, 1500
TauI GCSGC 1 cut(s) 64
Tru1I TTAA 2 cut(s) 648, 795
Tru9I TTAA 2 cut(s) 648, 795
TscAI CASTG 5 cut(s) 124, 136, 298, 336, 495
TseFI GTSAC 3 cut(s) 119, 815, 1091
TseI GCWGC 4 cut(s) 947, 959, 1216, 1627
Tsp45I GTSAC 3 cut(s) 119, 815, 1091
TspDTI ATGAA 1 cut(s) 932
TspRI CASTG 5 cut(s) 124, 136, 298, 336, 495
Tth111I GACNNNGTC 1 cut(s) 131
VneI GTGCAC 1 cut(s) 1599
VpaK11BI GGWCC 1 cut(s) 1724
XagI CCTNNNNNAGG 1 cut(s) 1375
XapI RAATTY 4 cut(s) 418, 527, 634, 970
XceI RCATGY 2 cut(s) 1168, 1625
XcmI CCANNNNNNNNNTGG 1 cut(s) 328
XhoI CTCGAG 1 cut(s) 275
XmiI GTMKAC 3 cut(s) 126, 135, 848
XspI CTAG 4 cut(s) 243, 456, 594, 1764
ZraI GACGTC 1 cut(s) 124
Zsp2I ATGCAT 1 cut(s) 1134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.