Rw0G022620

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig01050
OG0012017
Physical Location & Seq
Reverse (-)
33292 .. 34808
1517 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G022620.1

Sequence Viewer

Length: 1428 bp
ATGATTATGGCACTAAAAGCTAAACCCATCTCATCCCCATCACCATTTTTCAGTAGCTCCACCCGTTCCCAAAACTTGAAGCAAAACTTGAAATCGAGCGGTGTTTTCCTCTCGAATGATTTGATAGATGAGGTTCTGAAGCGTGCTAGGTTCAGCCATGGAAACCCTTTACAGGCTCTCGAGTTTTTCAATTACACTGGGAATAGGAGAGGATTTTATCACACCACATTTGCACTCGACACAATGCTTTATATGTTGGGAAGAAGTCGAATGTTCGAAAAGATGTGGGAGGTTTTGGTTGAGATCAAGAATAAGGATAGGAATTTGATAACTCCGCGTACTGTGATGGTTGTGTTAGCTAGGATTGCTAAGGTTTGTTCTGTTAGGGAGACTGTGGAGTGTTTCAGAAAGTTCAAGAAGCTTGTGCCCGAATTCGACACAGCTTGTTTCAATGCGTTGTTGAGGACTCTTTGTCAAGAGAAGAGTATGACAGATGCTAGGAATGTGTATCATAAGCTGAAGCAGAGCTTTAGGCCGAATTTGCAGACCTTTAATATATTGTTATCGGGTTGGAAGTCATCGGAGGAAGCTGAGGGGTTTTTCGAGGAGATGAGGGAGATGGGTCTGAAACCTGATATTGTTTCGTACAATTGTTTGATTGACGTTTATAGTAAGAATAGGGAAATGGAGAAAGCGTTTAAGGTGATGGAAACAATGCGTGACGAGGATATATCACCGGATAAGATCACGTATACTTGTGTCATTGGGGGACTGGGGTTGGTTGGTCAGCCTGATAAAGCCAGAGATGTGTTGAAGGAAATGAAGGAGCTTGGGTGCTACCCAGATGTTGCGGCATACAATGCTGCCATTAGGAATTTTTGCATTGCAAAGAGGCTTGGTGATGCTAATGGGTTGATGGAGGAGATGAGAAGTAATGGTTTGAGTCCAAATGCAACTACTTACAATTTGTTCTTTAGGGTTTTCTTCTGGTCAAGTGACTTGCAGAACTCTTGGAGCTTGTATGGGAGAATGATGCATACTGGGTGTCTGCCTAATACACAGTCGTGCATGTTCCTGATCAGGTTGTTTAGGAGGCTGGAAAAGGTGGATATGGCATTGCAGCTGTGGAATGATATGGTTGAGAAGGGTTTTGGGTCTTATATATTGGTATCTGATGTGCTGTTTGATTTGCTGTGTGATATGGGTAAACTAACAGAAGCAGAAACGTGCTTCTTGCAGATGGTGGAGAAGGCGCATAAGCCAAGTAATGTTTCCTTTAGAAGGATCAAGGTGCTTATGGAACTGGCAAACAAGCATGAGGCCCTCAAGAACTTGACAGAGAAAATGGCACTTTTTGGCTCTTCAATCCATCTTCCTGACATTATGGATAGGTCAACAGAGGAACCACACTCAGAACCACTTGCTTGA

Protein Analysis

475

Amino Acids

54.63

Weight (kDa)

8.68

Isoelectric Point (pI)

47.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 146 - 190 5.7e-07 PPR repeat family
PPR_3 PF13812 201 - 257 4.8e-11 Pentatricopeptide repeat domain
PPR_long PF17177 201 - 327 2.9e-10 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 204 - 290 5.1e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 207 - 240 1.2e-08 PPR repeat
PPR_2 PF13041 211 - 258 1.7e-16 PPR repeat family
PPR PF01535 214 - 243 5.2e-07 PPR repeat
PPR_3 PF13812 243 - 290 5.6e-06 Pentatricopeptide repeat domain
PPR PF01535 249 - 279 9.6e-07 PPR repeat
PPR_2 PF13041 252 - 294 2.1e-07 PPR repeat family
PPR_3 PF13812 270 - 327 3.8e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 281 - 328 1e-10 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012017)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02420
fragaria_vesca FvH4_5g32610 FvH4_5g32610 FvH4_5g32610
malus_domestica MD08G1216300.v1.1
prunus_persica Prupe.1G549200_v2.0.a1
pyrus_communis pycom08g18730
rosa_chinensis RchiOBHm_Chr7g0230241
rosa_laevigata RLG00000001507
rosa_multiflora Rmu_sc0002822.1_g000019 Rmu_sc0008506.1_g000024
rosa_roxburghii Rroxscaffold_3G00230280
rosa_rugosa Rorug07G0260000
rosa_samantha Rh7AG409400 Rh7BG389300 Rh7CG428000 Rh7DG407000
rosa_wichuraiana Rw0G018590 Rw0G022620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 99
AccI GTMKAC 1 cut(s) 752
AccII CGCG 1 cut(s) 337
AciI CCGC 3 cut(s) 99, 335, 851
AclWI GGATC 1 cut(s) 1292
AcsI RAATTY 4 cut(s) 322, 431, 538, 874
AcuI CTGAAG 2 cut(s) 158, 539
AfaI GTAC 2 cut(s) 340, 647
AfiI CCNNNNNNNGG 2 cut(s) 172, 1281
AgsI TTSAA 7 cut(s) 79, 91, 190, 415, 451, 814, 1365
AhdI GACNNNNNGTC 1 cut(s) 471
AjuI GAANNNNNNNTTGG 2 cut(s) 1255, 1287
AleI CACNNNNGTG 1 cut(s) 1063
Alw26I GTCTC 1 cut(s) 383
AlwI GGATC 1 cut(s) 1292
Ama87I CYCGRG 1 cut(s) 179
AoxI GGCC 2 cut(s) 533, 1320
ApeKI GCWGC 2 cut(s) 863, 1120
ApoI RAATTY 4 cut(s) 322, 431, 538, 874
AspLEI GCGC 1 cut(s) 1255
AspS9I GGNCC 1 cut(s) 1321
AsuHPI GGTGA 4 cut(s) 33, 715, 726, 911
AsuII TTCGAA 1 cut(s) 276
AvaI CYCGRG 1 cut(s) 179
BaeGI GKGCMC 1 cut(s) 429
BbvCI CCTCAGC 1 cut(s) 591
BbvI GCAGC 2 cut(s) 850, 1132
BccI CCATC 8 cut(s) 35, 46, 340, 613, 700, 910, 1234, 1377
BclI TGATCA 1 cut(s) 1077
BcoDI GTCTC 1 cut(s) 383
BfaI CTAG 3 cut(s) 147, 360, 498
BisI GCNGC 3 cut(s) 852, 864, 1121
BlsI GCNGC 3 cut(s) 853, 865, 1122
BmeRI GACNNNNNGTC 1 cut(s) 471
BmeT110I CYCGRG 1 cut(s) 179
BmgT120I GGNCC 1 cut(s) 1321
BmiI GGNNCC 1 cut(s) 1404
BmrI ACTGGG 3 cut(s) 207, 782, 1050
BmsI GCATC 3 cut(s) 484, 892, 1023
BmuI ACTGGG 3 cut(s) 207, 782, 1050
Bpu10I CCTNAGC 2 cut(s) 369, 591
Bpu14I TTCGAA 1 cut(s) 276
BpuEI CTTGAG 1 cut(s) 1310
BsaAI YACGTR 1 cut(s) 750
BsaJI CCNNGG 1 cut(s) 157
BsaWI WCCGGW 1 cut(s) 736
BsaXI ACNNNNNCTCC 4 cut(s) 680, 710, 818, 848
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 1281
Bse1I ACTGG 4 cut(s) 202, 777, 1045, 1308
Bse3DI GCAATG 2 cut(s) 882, 1115
BseDI CCNNGG 1 cut(s) 157
BseGI GGATG 1 cut(s) 32
BseLI CCNNNNNNNGG 2 cut(s) 172, 1281
BseMI GCAATG 2 cut(s) 882, 1115
BseMII CTCAG 2 cut(s) 582, 1425
BseNI ACTGG 4 cut(s) 202, 777, 1045, 1308
BseRI GAGGAG 2 cut(s) 620, 935
BseSI GKGCMC 1 cut(s) 429
BseXI GCAGC 2 cut(s) 850, 1132
Bsh1236I CGCG 1 cut(s) 337
BshFI GGCC 2 cut(s) 535, 1322
BsiHKCI CYCGRG 1 cut(s) 179
BsiSI CCGG 1 cut(s) 737
BslFI GGGAC 1 cut(s) 783
BslI CCNNNNNNNGG 2 cut(s) 172, 1281
BsmAI GTCTC 1 cut(s) 383
BsmFI GGGAC 1 cut(s) 783
BsnI GGCC 2 cut(s) 535, 1322
BsoBI CYCGRG 1 cut(s) 179
Bsp119I TTCGAA 1 cut(s) 276
Bsp1286I GDGCHC 1 cut(s) 429
Bsp143I GATC 4 cut(s) 303, 744, 1077, 1284
Bsp19I CCATGG 1 cut(s) 157
BspACI CCGC 3 cut(s) 99, 335, 851
BspANI GGCC 2 cut(s) 535, 1322
BspCNI CTCAG 2 cut(s) 583, 1424
BspFNI CGCG 1 cut(s) 337
BspLI GGNNCC 1 cut(s) 1404
BspPI GGATC 1 cut(s) 1292
BspQI GCTCTTC 1 cut(s) 1366
BspT104I TTCGAA 1 cut(s) 276
BsrBI CCGCTC 1 cut(s) 99
BsrDI GCAATG 2 cut(s) 882, 1115
BsrI ACTGG 4 cut(s) 202, 777, 1045, 1308
BssECI CCNNGG 1 cut(s) 157
BssMI GATC 4 cut(s) 303, 744, 1077, 1284
BssNAI GTATAC 1 cut(s) 753
BssT1I CCWWGG 1 cut(s) 157
Bst1107I GTATAC 1 cut(s) 753
Bst4CI ACNGT 3 cut(s) 343, 394, 1062
Bst6I CTCTTC 2 cut(s) 476, 1366
BstAPI GCANNNNNTGC 1 cut(s) 860
BstBAI YACGTR 1 cut(s) 750
BstBI TTCGAA 1 cut(s) 276
BstC8I GCNNGC 1 cut(s) 144
BstDEI CTNAG 3 cut(s) 369, 591, 1411
BstDSI CCRYGG 1 cut(s) 157
BstENI CCTNNNNNAGG 1 cut(s) 1279
BstF5I GGATG 1 cut(s) 32
BstFNI CGCG 1 cut(s) 337
BstHHI GCGC 1 cut(s) 1255
BstKTI GATC 4 cut(s) 306, 747, 1080, 1287
BstMAI GTCTC 1 cut(s) 383
BstMBI GATC 4 cut(s) 303, 744, 1077, 1284
BstMWI GCNNNNNNNGC 4 cut(s) 17, 365, 541, 860
BstNSI RCATGY 1 cut(s) 1072
BstSLI GKGCMC 1 cut(s) 429
BstUI CGCG 1 cut(s) 337
BstV1I GCAGC 2 cut(s) 850, 1132
BstZ17I GTATAC 1 cut(s) 753
BsuRI GGCC 2 cut(s) 535, 1322
BtgI CCRYGG 1 cut(s) 157
BtsCI GGATG 1 cut(s) 32
BtsIMutI CAGTG 1 cut(s) 195
Cac8I GCNNGC 1 cut(s) 144
CfoI GCGC 1 cut(s) 1255
Cfr13I GGNCC 1 cut(s) 1321
Csp6I GTAC 2 cut(s) 339, 646
CviAII CATG 3 cut(s) 158, 1069, 1316
CviQI GTAC 2 cut(s) 339, 646
DdeI CTNAG 3 cut(s) 369, 591, 1411
DpnI GATC 4 cut(s) 305, 746, 1079, 1286
DpnII GATC 4 cut(s) 303, 744, 1077, 1284
DriI GACNNNNNGTC 1 cut(s) 471
Eam1104I CTCTTC 2 cut(s) 476, 1366
Eam1105I GACNNNNNGTC 1 cut(s) 471
EarI CTCTTC 2 cut(s) 476, 1366
Eco130I CCWWGG 1 cut(s) 157
Eco57I CTGAAG 2 cut(s) 158, 539
Eco88I CYCGRG 1 cut(s) 179
EcoNI CCTNNNNNAGG 1 cut(s) 1279
EcoO109I RGGNCCY 1 cut(s) 1321
EcoRI GAATTC 1 cut(s) 431
EcoT14I CCWWGG 1 cut(s) 157
EcoT22I ATGCAT 1 cut(s) 1038
ErhI CCWWGG 1 cut(s) 157
FaeI CATG 3 cut(s) 161, 1072, 1319
FalI AAGNNNNNCTT 4 cut(s) 71, 103, 512, 544
FaqI GGGAC 1 cut(s) 783
FatI CATG 3 cut(s) 157, 1068, 1315
FbaI TGATCA 1 cut(s) 1077
FblI GTMKAC 1 cut(s) 752
Fnu4HI GCNGC 3 cut(s) 852, 864, 1121
FokI GGATG 1 cut(s) 19
Fsp4HI GCNGC 3 cut(s) 852, 864, 1121
FspBI CTAG 3 cut(s) 147, 360, 498
GlaI GCGC 1 cut(s) 1254
GluI GCNGC 3 cut(s) 852, 864, 1121
HaeIII GGCC 2 cut(s) 535, 1322
HapII CCGG 1 cut(s) 737
HhaI GCGC 1 cut(s) 1255
Hin1II CATG 3 cut(s) 161, 1072, 1319
Hin6I GCGC 1 cut(s) 1253
HinP1I GCGC 1 cut(s) 1253
HincII GTYRAC 1 cut(s) 1395
HindII GTYRAC 1 cut(s) 1395
HindIII AAGCTT 1 cut(s) 419
HinfI GANTC 2 cut(s) 466, 943
HpaII CCGG 1 cut(s) 737
HphI GGTGA 4 cut(s) 33, 715, 726, 911
Hpy166II GTNNAC 3 cut(s) 753, 1208, 1395
Hpy188I TCNGA 6 cut(s) 138, 407, 583, 627, 1174, 1414
Hpy188III TCNNGA 8 cut(s) 112, 179, 307, 415, 476, 1075, 1327, 1376
Hpy8I GTNNAC 3 cut(s) 753, 1208, 1395
HpyAV CCTTC 5 cut(s) 808, 817, 1138, 1243, 1275
HpyCH4III ACNGT 3 cut(s) 343, 394, 1062
HpyCH4IV ACGT 3 cut(s) 663, 749, 1226
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 365, 541, 860
HpyF3I CTNAG 3 cut(s) 369, 591, 1411
HpySE526I ACGT 3 cut(s) 663, 749, 1226
Hsp92II CATG 3 cut(s) 161, 1072, 1319
HspAI GCGC 1 cut(s) 1253
Ksp22I TGATCA 1 cut(s) 1077
Kzo9I GATC 4 cut(s) 303, 744, 1077, 1284
LguI GCTCTTC 1 cut(s) 1366
LmnI GCTCC 3 cut(s) 62, 826, 1014
Lsp1109I GCAGC 2 cut(s) 850, 1132
LweI GCATC 3 cut(s) 484, 892, 1023
MaeI CTAG 3 cut(s) 147, 360, 498
MaeII ACGT 3 cut(s) 663, 749, 1226
MaeIII GTNAC 2 cut(s) 719, 995
MalI GATC 4 cut(s) 305, 746, 1079, 1286
MbiI CCGCTC 1 cut(s) 99
MboI GATC 4 cut(s) 303, 744, 1077, 1284
MboII GAAGA 5 cut(s) 273, 493, 976, 1353, 1364
MfeI CAATTG 1 cut(s) 649
MhlI GDGCHC 1 cut(s) 429
MluCI AATT 7 cut(s) 190, 322, 431, 538, 649, 874, 964
MlyI GAGTC 2 cut(s) 460, 952
MmeI TCCRAC 1 cut(s) 551
Mph1103I ATGCAT 1 cut(s) 1038
MseI TTAA 2 cut(s) 552, 699
MslI CAYNNNNRTG 1 cut(s) 1063
MspA1I CMGCKG 1 cut(s) 1123
MspI CCGG 1 cut(s) 737
MunI CAATTG 1 cut(s) 649
MvnI CGCG 1 cut(s) 337
MwoI GCNNNNNNNGC 4 cut(s) 17, 365, 541, 860
NcoI CCATGG 1 cut(s) 157
NdeII GATC 4 cut(s) 303, 744, 1077, 1284
NlaIII CATG 3 cut(s) 161, 1072, 1319
NlaIV GGNNCC 1 cut(s) 1404
NmuCI GTSAC 2 cut(s) 719, 995
NsiI ATGCAT 1 cut(s) 1038
NspI RCATGY 1 cut(s) 1072
NspV TTCGAA 1 cut(s) 276
OliI CACNNNNGTG 1 cut(s) 1063
PaeR7I CTCGAG 1 cut(s) 179
PciSI GCTCTTC 1 cut(s) 1366
PkrI GCNGC 3 cut(s) 853, 865, 1122
PleI GAGTC 2 cut(s) 460, 951
PpsI GAGTC 2 cut(s) 460, 951
Ppu21I YACGTR 1 cut(s) 750
PspN4I GGNNCC 1 cut(s) 1404
PspPI GGNCC 1 cut(s) 1321
PvuII CAGCTG 1 cut(s) 1123
RsaI GTAC 2 cut(s) 340, 647
RsaNI GTAC 2 cut(s) 339, 646
RseI CAYNNNNRTG 1 cut(s) 1063
SapI GCTCTTC 1 cut(s) 1366
SaqAI TTAA 2 cut(s) 552, 699
SatI GCNGC 3 cut(s) 852, 864, 1121
Sau3AI GATC 4 cut(s) 303, 744, 1077, 1284
Sau96I GGNCC 1 cut(s) 1321
SchI GAGTC 2 cut(s) 460, 952
SduI GDGCHC 1 cut(s) 429
SfaNI GCATC 3 cut(s) 484, 892, 1023
Sfr274I CTCGAG 1 cut(s) 179
SfuI TTCGAA 1 cut(s) 276
SlaI CTCGAG 1 cut(s) 179
SmiMI CAYNNNNRTG 1 cut(s) 1063
SmlI CTYRAG 2 cut(s) 179, 1325
SmoI CTYRAG 2 cut(s) 179, 1325
Sse9I AATT 7 cut(s) 190, 322, 431, 538, 649, 874, 964
SsiI CCGC 3 cut(s) 99, 335, 851
SspMI CTAG 3 cut(s) 147, 360, 498
StyI CCWWGG 1 cut(s) 157
TaaI ACNGT 3 cut(s) 343, 394, 1062
TaiI ACGT 3 cut(s) 666, 752, 1229
TaqI TCGA 8 cut(s) 95, 113, 180, 237, 268, 276, 435, 603
TasI AATT 7 cut(s) 190, 322, 431, 538, 649, 874, 964
TauI GCSGC 1 cut(s) 854
Tru1I TTAA 2 cut(s) 552, 699
Tru9I TTAA 2 cut(s) 552, 699
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 2 cut(s) 719, 995
TseI GCWGC 2 cut(s) 863, 1120
Tsp45I GTSAC 2 cut(s) 719, 995
TspDTI ATGAA 1 cut(s) 836
TspRI CASTG 1 cut(s) 202
XagI CCTNNNNNAGG 1 cut(s) 1279
XapI RAATTY 4 cut(s) 322, 431, 538, 874
XceI RCATGY 1 cut(s) 1072
XhoI CTCGAG 1 cut(s) 179
XmiI GTMKAC 1 cut(s) 752
XspI CTAG 3 cut(s) 147, 360, 498
Zsp2I ATGCAT 1 cut(s) 1038
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.