RchiOBHm_Chr7g0230241

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0012017
Physical Location & Seq
Reverse (-)
53658492 .. 53660564
2073 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20629

Sequence Viewer

Length: 1740 bp
ATGTTTTGGGTTGATTTTTTTGCTTACGTGGAATTCTTGTGCATCCGCATCACCATTGTTCCGTTGTTCATATATTGCAGCCCTCGCCCCAACTGGACAATAAAACGCTCCAAGGTTTTACGGCCCATCTCATTGCAACAGTGTTCTCTCCAGAACCCAGTGCAAAACAGAGCTGCAATTCATTTCTGGGTCAATCAAGCTTTTATTTCAACGGAGATGATGATGGCACTAAAAGCTAAACCCCTCTCATCCCCATCACCATTTTTCAGGTACTCGCCGCAAAACCCAATTTTCTTTCTCCCTCGATTACTCTCTTCCCAAACCCCAATCGACAGTGACGTCGACACTGTCTACCGCATAGTCAGTAGCTCCACCCGTTCCCAAAACTTGAAGCAAAACTTGAAATCGAGCGGTGTTTTCCTCTCAAATGATTTGATAGATGAGGTTCTGAAGCGGGCCAGGTTCAGCCATGGAAACCCTTTACACGCTCTCGAGTTTTTCAATTACACTGGGAATAGGAGAGGGTTTTATCACACCACATTTGCACTTGACACAATGCTTTATATGTTGGGAAGAAGTCGAATGTTCGAAAAGATGTGGGAGGTTTTGGTTGAGATCAAGAGTAAGGATAGGAATTTGATAACTCCTCGTACTGTGATGGTTGTTTTAGCTAGGATTGCTAAGGTTTGTTCTGTTAGGGAGACTGTGGAGTGTTTTAGAGAGTTCAAGAAGCTTGTGCCCGAATTCGACACAGCTTGTTTCAATGCGTTGTTGAGGACTCTTTGTCAAGAGAAGAGTATGACAGATGCTAGGAATGTGTATCATAAGCTGAAGCAGAGCTTTAGGCCGAATTTGCAGACCTTTAATATATTGTTATCGGGTTGGAAGTCATCGGAGGATGCTGAGGGGTTTTTCGAGGAGATGGGGGAGATTGGTCTGAAACCTGATATTGTTTCGTACAATTGTTTGATCGATGTTTATAGTAAGAATAGGGAAATGGAGAAAGCGTTTAAGTTGATGGAAAAAATGCATGACGAGGATATATCACCGGATAAGATCACGTATACTTGTGTCATTGGGGGATTGGGGTTGGTTGGTCAGCCTGATAAAGCCAGAGATGTGTTGAAGGAAATGAAGGAGCTTGGGTGCTACCCAGATGTTGCGGCATACAATGCTGCCATTAGGAATTTTTGCATTGCAAAGAGGCTTGGTGATGCTAATGGGTTGGTGGAGGAGATGATAAGTAATGGTTTGAGTCCAAATGCAACTACTTACAATTTGTTCTTTAGGGTTTTCTTCTGGTCAAGTGACTTGCAGAACTCTTGGAGCTTGTATGGGAGAATGATGCATACTGGGTGTCTGCCAAATACACAGTCGTGCATGTTCCTGATCAGGTTGTTTAAGAGGCTGGAAAAGGTGGATATGGCATTGCAGCTGTGGAATGAAATGGTGGAGAGGGGTTTTGGGTCTTATATATTGGTATCTGATGTGCTGTTTGATTTGCTGTGTGATATGGGTAAACTAACAGAAGCAGAAACGTGCTTCTTGCAGATGGTGGAGAAGGGGCATAAGCCAAGTAATGTTTCCTTTAGAAGGATCAAGGTGCTTATGGAACTGGCAAACAAGCATGAGGCCCTCAAGAACTTGACAGAGAAAATGGCACTTTTTGGCTCTTCAATCCATCTTCCTGAGAGTATGGATAGGTCAACAGAGGTACCACGCTCAGAACCACTTGCTTGA

Protein Analysis

579

Amino Acids

66.72

Weight (kDa)

8.74

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 250 - 294 7.4e-07 PPR repeat family
PPR_3 PF13812 305 - 361 3.2e-09 Pentatricopeptide repeat domain
TPR_24 PF23276 309 - 406 1.7e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 312 - 343 8.8e-09 PPR repeat
PPR_2 PF13041 315 - 362 1.1e-16 PPR repeat family
PPR PF01535 318 - 347 1.1e-07 PPR repeat
PPR_long PF17177 319 - 431 1.2e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 338 - 394 1.2e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 353 - 398 1.3e-09 PPR repeat family
PPR PF01535 353 - 383 1.2e-06 PPR repeat
PPR_3 PF13812 374 - 431 6.1e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 385 - 432 1.2e-09 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012017)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02420
fragaria_vesca FvH4_5g32610 FvH4_5g32610 FvH4_5g32610
malus_domestica MD08G1216300.v1.1
prunus_persica Prupe.1G549200_v2.0.a1
pyrus_communis pycom08g18730
rosa_chinensis RchiOBHm_Chr7g0230241
rosa_laevigata RLG00000001507
rosa_multiflora Rmu_sc0002822.1_g000019 Rmu_sc0008506.1_g000024
rosa_roxburghii Rroxscaffold_3G00230280
rosa_rugosa Rorug07G0260000
rosa_samantha Rh7AG409400 Rh7BG389300 Rh7CG428000 Rh7DG407000
rosa_wichuraiana Rw0G018590 Rw0G022620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 338
AatII GACGTC 1 cut(s) 342
Acc65I GGTACC 1 cut(s) 1714
AccB1I GGYRCC 1 cut(s) 1714
AccBSI CCGCTC 1 cut(s) 411
AccI GTMKAC 3 cut(s) 342, 351, 1064
AciI CCGC 6 cut(s) 46, 278, 355, 411, 454, 1163
AclWI GGATC 1 cut(s) 1604
AcsI RAATTY 5 cut(s) 32, 634, 743, 850, 1186
AcuI CTGAAG 2 cut(s) 470, 851
AcyI GRCGYC 1 cut(s) 339
AfaI GTAC 4 cut(s) 272, 652, 959, 1716
AfiI CCNNNNNNNGG 1 cut(s) 1593
AgsI TTSAA 8 cut(s) 210, 391, 403, 502, 727, 763, 1126, 1677
AhdI GACNNNNNGTC 1 cut(s) 783
AjnI CCWGG 1 cut(s) 458
AjuI GAANNNNNNNTTGG 2 cut(s) 1567, 1599
AleI CACNNNNGTG 1 cut(s) 1375
Alw26I GTCTC 1 cut(s) 695
AlwI GGATC 1 cut(s) 1604
Ama87I CYCGRG 1 cut(s) 491
AoxI GGCC 4 cut(s) 122, 456, 845, 1632
ApeKI GCWGC 4 cut(s) 78, 173, 1175, 1432
ApoI RAATTY 5 cut(s) 32, 634, 743, 850, 1186
ArsI GACNNNNNNTTYG 2 cut(s) 1358, 1390
Asp718I GGTACC 1 cut(s) 1714
AspS9I GGNCC 3 cut(s) 123, 456, 1633
AsuHPI GGTGA 4 cut(s) 43, 249, 1038, 1223
AsuII TTCGAA 1 cut(s) 588
AvaI CYCGRG 1 cut(s) 491
BaeGI GKGCMC 1 cut(s) 741
BaeI ACNNNNGTAYC 2 cut(s) 1698, 1731
BanI GGYRCC 1 cut(s) 1714
BbvCI CCTCAGC 1 cut(s) 903
BbvI GCAGC 4 cut(s) 90, 160, 1162, 1444
BccI CCATC 8 cut(s) 134, 217, 262, 652, 916, 1012, 1546, 1689
BceAI ACGGC 1 cut(s) 137
BciT130I CCWGG 1 cut(s) 460
BclI TGATCA 1 cut(s) 1389
BcoDI GTCTC 1 cut(s) 695
BfaI CTAG 2 cut(s) 672, 810
BisI GCNGC 6 cut(s) 79, 174, 278, 1164, 1176, 1433
BlsI GCNGC 6 cut(s) 80, 175, 279, 1165, 1177, 1434
Bme1390I CCNGG 1 cut(s) 460
BmeRI GACNNNNNGTC 1 cut(s) 783
BmeT110I CYCGRG 1 cut(s) 491
BmgT120I GGNCC 3 cut(s) 123, 456, 1633
BmiI GGNNCC 1 cut(s) 1716
BmrFI CCNGG 1 cut(s) 460
BmrI ACTGGG 3 cut(s) 152, 519, 1362
BmsI GCATC 6 cut(s) 51, 57, 796, 889, 1204, 1335
BmuI ACTGGG 3 cut(s) 152, 519, 1362
BpmI CTGGAG 1 cut(s) 134
Bpu10I CCTNAGC 2 cut(s) 681, 903
Bpu14I TTCGAA 1 cut(s) 588
BpuEI CTTGAG 1 cut(s) 1622
Bsa29I ATCGAT 1 cut(s) 972
BsaAI YACGTR 2 cut(s) 28, 1062
BsaHI GRCGYC 1 cut(s) 339
BsaJI CCNNGG 2 cut(s) 111, 469
BsaWI WCCGGW 1 cut(s) 1048
BsaXI ACNNNNNCTCC 4 cut(s) 992, 1022, 1130, 1160
Bsc4I CCNNNNNNNGG 1 cut(s) 1593
Bse1I ACTGG 5 cut(s) 98, 158, 514, 1357, 1620
Bse3DI GCAATG 3 cut(s) 131, 1194, 1427
BseBI CCWGG 1 cut(s) 460
BseCI ATCGAT 1 cut(s) 972
BseDI CCNNGG 2 cut(s) 111, 469
BseGI GGATG 3 cut(s) 42, 248, 904
BseLI CCNNNNNNNGG 1 cut(s) 1593
BseMI GCAATG 3 cut(s) 131, 1194, 1427
BseMII CTCAG 3 cut(s) 894, 1680, 1737
BseNI ACTGG 5 cut(s) 98, 158, 514, 1357, 1620
BseRI GAGGAG 3 cut(s) 636, 932, 1247
BseSI GKGCMC 1 cut(s) 741
BseXI GCAGC 4 cut(s) 90, 160, 1162, 1444
BshFI GGCC 4 cut(s) 124, 458, 847, 1634
BshNI GGYRCC 1 cut(s) 1714
BshVI ATCGAT 1 cut(s) 972
BsiHKCI CYCGRG 1 cut(s) 491
BsiSI CCGG 1 cut(s) 1049
BslI CCNNNNNNNGG 1 cut(s) 1593
BsmAI GTCTC 1 cut(s) 695
BsnI GGCC 4 cut(s) 124, 458, 847, 1634
BsoBI CYCGRG 1 cut(s) 491
Bsp119I TTCGAA 1 cut(s) 588
Bsp1286I GDGCHC 1 cut(s) 741
Bsp143I GATC 5 cut(s) 615, 969, 1056, 1389, 1596
Bsp19I CCATGG 1 cut(s) 469
BspACI CCGC 6 cut(s) 46, 278, 355, 411, 454, 1163
BspANI GGCC 4 cut(s) 124, 458, 847, 1634
BspCNI CTCAG 3 cut(s) 895, 1681, 1736
BspDI ATCGAT 1 cut(s) 972
BspLI GGNNCC 1 cut(s) 1716
BspPI GGATC 1 cut(s) 1604
BspQI GCTCTTC 1 cut(s) 1678
BspT104I TTCGAA 1 cut(s) 588
BspT107I GGYRCC 1 cut(s) 1714
BsrBI CCGCTC 1 cut(s) 411
BsrDI GCAATG 3 cut(s) 131, 1194, 1427
BsrI ACTGG 5 cut(s) 98, 158, 514, 1357, 1620
BssECI CCNNGG 2 cut(s) 111, 469
BssMI GATC 5 cut(s) 615, 969, 1056, 1389, 1596
BssNAI GTATAC 1 cut(s) 1065
BssNI GRCGYC 1 cut(s) 339
BssT1I CCWWGG 2 cut(s) 111, 469
Bst1107I GTATAC 1 cut(s) 1065
Bst2UI CCWGG 1 cut(s) 460
Bst4CI ACNGT 6 cut(s) 141, 335, 349, 655, 706, 1374
Bst6I CTCTTC 3 cut(s) 319, 788, 1678
BstACI GRCGYC 1 cut(s) 339
BstAPI GCANNNNNTGC 1 cut(s) 1172
BstBAI YACGTR 2 cut(s) 28, 1062
BstBI TTCGAA 1 cut(s) 588
BstC8I GCNNGC 1 cut(s) 456
BstDEI CTNAG 4 cut(s) 681, 903, 1689, 1723
BstDSI CCRYGG 1 cut(s) 469
BstENI CCTNNNNNAGG 1 cut(s) 1591
BstF5I GGATG 3 cut(s) 42, 248, 904
BstKTI GATC 5 cut(s) 618, 972, 1059, 1392, 1599
BstMAI GTCTC 1 cut(s) 695
BstMBI GATC 5 cut(s) 615, 969, 1056, 1389, 1596
BstMWI GCNNNNNNNGC 5 cut(s) 84, 233, 677, 853, 1172
BstNI CCWGG 1 cut(s) 460
BstNSI RCATGY 1 cut(s) 1384
BstSCI CCNGG 1 cut(s) 458
BstSLI GKGCMC 1 cut(s) 741
BstV1I GCAGC 4 cut(s) 90, 160, 1162, 1444
BstZ17I GTATAC 1 cut(s) 1065
Bsu15I ATCGAT 1 cut(s) 972
BsuRI GGCC 4 cut(s) 124, 458, 847, 1634
BsuTUI ATCGAT 1 cut(s) 972
BtgI CCRYGG 1 cut(s) 469
BtsCI GGATG 3 cut(s) 42, 248, 904
BtsIMutI CAGTG 5 cut(s) 146, 165, 340, 345, 507
Cac8I GCNNGC 1 cut(s) 456
Cfr13I GGNCC 3 cut(s) 123, 456, 1633
ClaI ATCGAT 1 cut(s) 972
Csp6I GTAC 4 cut(s) 271, 651, 958, 1715
CviAII CATG 4 cut(s) 470, 1031, 1381, 1628
CviQI GTAC 4 cut(s) 271, 651, 958, 1715
DdeI CTNAG 4 cut(s) 681, 903, 1689, 1723
DpnI GATC 5 cut(s) 617, 971, 1058, 1391, 1598
DpnII GATC 5 cut(s) 615, 969, 1056, 1389, 1596
DrdI GACNNNNNNGTC 1 cut(s) 338
DriI GACNNNNNGTC 1 cut(s) 783
DseDI GACNNNNNNGTC 1 cut(s) 338
Eam1104I CTCTTC 3 cut(s) 319, 788, 1678
Eam1105I GACNNNNNGTC 1 cut(s) 783
EarI CTCTTC 3 cut(s) 319, 788, 1678
Eco130I CCWWGG 2 cut(s) 111, 469
Eco57I CTGAAG 2 cut(s) 470, 851
Eco88I CYCGRG 1 cut(s) 491
EcoNI CCTNNNNNAGG 1 cut(s) 1591
EcoO109I RGGNCCY 1 cut(s) 1633
EcoRI GAATTC 2 cut(s) 32, 743
EcoRII CCWGG 1 cut(s) 458
EcoT14I CCWWGG 2 cut(s) 111, 469
EcoT22I ATGCAT 2 cut(s) 1032, 1350
ErhI CCWWGG 2 cut(s) 111, 469
FaeI CATG 4 cut(s) 473, 1034, 1384, 1631
FalI AAGNNNNNCTT 4 cut(s) 383, 415, 824, 856
FatI CATG 4 cut(s) 469, 1030, 1380, 1627
FauI CCCGC 1 cut(s) 447
FbaI TGATCA 1 cut(s) 1389
FblI GTMKAC 3 cut(s) 342, 351, 1064
Fnu4HI GCNGC 6 cut(s) 79, 174, 278, 1164, 1176, 1433
FokI GGATG 3 cut(s) 29, 235, 911
Fsp4HI GCNGC 6 cut(s) 79, 174, 278, 1164, 1176, 1433
FspBI CTAG 2 cut(s) 672, 810
GluI GCNGC 6 cut(s) 79, 174, 278, 1164, 1176, 1433
GsuI CTGGAG 1 cut(s) 134
HaeIII GGCC 4 cut(s) 124, 458, 847, 1634
HapII CCGG 1 cut(s) 1049
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 4 cut(s) 473, 1034, 1384, 1631
HincII GTYRAC 2 cut(s) 343, 1707
HindII GTYRAC 2 cut(s) 343, 1707
HindIII AAGCTT 2 cut(s) 198, 731
HinfI GANTC 2 cut(s) 778, 1255
HpaII CCGG 1 cut(s) 1049
HphI GGTGA 4 cut(s) 43, 249, 1038, 1223
Hpy166II GTNNAC 5 cut(s) 343, 352, 1065, 1520, 1707
Hpy188I TCNGA 5 cut(s) 450, 895, 939, 1486, 1726
Hpy188III TCNNGA 8 cut(s) 151, 491, 619, 727, 788, 1387, 1639, 1688
Hpy8I GTNNAC 5 cut(s) 343, 352, 1065, 1520, 1707
Hpy99I CGWCG 1 cut(s) 344
HpyAV CCTTC 4 cut(s) 1120, 1129, 1555, 1587
HpyCH4III ACNGT 6 cut(s) 141, 335, 349, 655, 706, 1374
HpyCH4IV ACGT 4 cut(s) 27, 339, 1061, 1538
HpyF10VI GCNNNNNNNGC 5 cut(s) 84, 233, 677, 853, 1172
HpyF3I CTNAG 4 cut(s) 681, 903, 1689, 1723
HpySE526I ACGT 4 cut(s) 27, 339, 1061, 1538
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 4 cut(s) 473, 1034, 1384, 1631
KpnI GGTACC 1 cut(s) 1718
Ksp22I TGATCA 1 cut(s) 1389
Kzo9I GATC 5 cut(s) 615, 969, 1056, 1389, 1596
LguI GCTCTTC 1 cut(s) 1678
LmnI GCTCC 4 cut(s) 113, 374, 1138, 1326
Lsp1109I GCAGC 4 cut(s) 90, 160, 1162, 1444
LweI GCATC 6 cut(s) 51, 57, 796, 889, 1204, 1335
MaeI CTAG 2 cut(s) 672, 810
MaeII ACGT 4 cut(s) 27, 339, 1061, 1538
MaeIII GTNAC 2 cut(s) 335, 1307
MalI GATC 5 cut(s) 617, 971, 1058, 1391, 1598
MbiI CCGCTC 1 cut(s) 411
MboI GATC 5 cut(s) 615, 969, 1056, 1389, 1596
MboII GAAGA 6 cut(s) 306, 585, 805, 1288, 1665, 1676
MfeI CAATTG 1 cut(s) 961
MhlI GDGCHC 1 cut(s) 741
MlyI GAGTC 2 cut(s) 772, 1264
MmeI TCCRAC 1 cut(s) 863
Mph1103I ATGCAT 2 cut(s) 1032, 1350
MseI TTAA 3 cut(s) 864, 1011, 1401
MslI CAYNNNNRTG 1 cut(s) 1375
MspA1I CMGCKG 1 cut(s) 1435
MspI CCGG 1 cut(s) 1049
MspR9I CCNGG 1 cut(s) 460
MunI CAATTG 1 cut(s) 961
MvaI CCWGG 1 cut(s) 460
MwoI GCNNNNNNNGC 5 cut(s) 84, 233, 677, 853, 1172
NcoI CCATGG 1 cut(s) 469
NdeII GATC 5 cut(s) 615, 969, 1056, 1389, 1596
NlaIII CATG 4 cut(s) 473, 1034, 1384, 1631
NlaIV GGNNCC 1 cut(s) 1716
NmuCI GTSAC 2 cut(s) 335, 1307
NsiI ATGCAT 2 cut(s) 1032, 1350
NspI RCATGY 1 cut(s) 1384
NspV TTCGAA 1 cut(s) 588
OliI CACNNNNGTG 1 cut(s) 1375
PaeR7I CTCGAG 1 cut(s) 491
PciSI GCTCTTC 1 cut(s) 1678
PcsI WCGNNNNNNNCGW 1 cut(s) 336
PflFI GACNNNGTC 1 cut(s) 347
PkrI GCNGC 6 cut(s) 80, 175, 279, 1165, 1177, 1434
PleI GAGTC 2 cut(s) 772, 1263
PpsI GAGTC 2 cut(s) 772, 1263
Ppu21I YACGTR 2 cut(s) 28, 1062
Psp6I CCWGG 1 cut(s) 458
PspGI CCWGG 1 cut(s) 458
PspN4I GGNNCC 1 cut(s) 1716
PspPI GGNCC 3 cut(s) 123, 456, 1633
PsyI GACNNNGTC 1 cut(s) 347
PvuII CAGCTG 1 cut(s) 1435
RsaI GTAC 4 cut(s) 272, 652, 959, 1716
RsaNI GTAC 4 cut(s) 271, 651, 958, 1715
RseI CAYNNNNRTG 1 cut(s) 1375
SalI GTCGAC 1 cut(s) 341
SapI GCTCTTC 1 cut(s) 1678
SaqAI TTAA 3 cut(s) 864, 1011, 1401
SatI GCNGC 6 cut(s) 79, 174, 278, 1164, 1176, 1433
Sau3AI GATC 5 cut(s) 615, 969, 1056, 1389, 1596
Sau96I GGNCC 3 cut(s) 123, 456, 1633
SchI GAGTC 2 cut(s) 772, 1264
ScrFI CCNGG 1 cut(s) 460
SduI GDGCHC 1 cut(s) 741
SfaNI GCATC 6 cut(s) 51, 57, 796, 889, 1204, 1335
Sfr274I CTCGAG 1 cut(s) 491
SfuI TTCGAA 1 cut(s) 588
SlaI CTCGAG 1 cut(s) 491
SmiMI CAYNNNNRTG 1 cut(s) 1375
SmlI CTYRAG 2 cut(s) 491, 1637
SmoI CTYRAG 2 cut(s) 491, 1637
SsiI CCGC 6 cut(s) 46, 278, 355, 411, 454, 1163
SspMI CTAG 2 cut(s) 672, 810
StyD4I CCNGG 1 cut(s) 458
StyI CCWWGG 2 cut(s) 111, 469
TaaI ACNGT 6 cut(s) 141, 335, 349, 655, 706, 1374
TaiI ACGT 4 cut(s) 30, 342, 1064, 1541
TauI GCSGC 2 cut(s) 280, 1166
Tru1I TTAA 3 cut(s) 864, 1011, 1401
Tru9I TTAA 3 cut(s) 864, 1011, 1401
TscAI CASTG 5 cut(s) 146, 165, 340, 352, 514
TseFI GTSAC 2 cut(s) 335, 1307
TseI GCWGC 4 cut(s) 78, 173, 1175, 1432
Tsp45I GTSAC 2 cut(s) 335, 1307
TspDTI ATGAA 4 cut(s) 58, 170, 1148, 1458
TspGWI ACGGA 2 cut(s) 51, 227
TspRI CASTG 5 cut(s) 146, 165, 340, 352, 514
Tth111I GACNNNGTC 1 cut(s) 347
XagI CCTNNNNNAGG 1 cut(s) 1591
XapI RAATTY 5 cut(s) 32, 634, 743, 850, 1186
XceI RCATGY 1 cut(s) 1384
XhoI CTCGAG 1 cut(s) 491
XmiI GTMKAC 3 cut(s) 342, 351, 1064
XspI CTAG 2 cut(s) 672, 810
ZraI GACGTC 1 cut(s) 340
Zsp2I ATGCAT 2 cut(s) 1032, 1350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.