MD08G1225000.v1.1

Transmembrane protein 87B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011281
Physical Location & Seq
Forward (+)
29009809 .. 29013887
4079 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1225000.v1.1.491

Sequence Viewer

Length: 1566 bp
ATGGATTTCACAATCCAGGTTAGAGCAACGAGGAGAAGAAGAAGAAGCAACGGCATTCCCCATGGCATTTTCTTGATGCTGTCTCTGCTCCTCCTGCTATCATTCCTTCCAAACCCCACAAACGCCTCGATCCACGTCTACGACAACCAAACCTTCAGAGAAGTCGGCAACGCCAACCTTCTCTCCGGCGGCAGCGAAGGCATCGCCGCCACTCTCTCCGCCCGTTCTTACATTCGATTCGAGAATATCACCTTCTGGAGGAGCAAGGATGCTGCTGAGAAGCTTTCAGACATGGAGCATAGTACTGGGTTGATACAAGTTATAATATTTGAGACAGCTGATCGGAATAATATCGGTGGTTCTGCTTATGGCGGACAAAGAGCTATATGTTGCACTCCTGATCTTGCTAAGATGGAAGGTTGTAAGCAAGGTGAAGTCATCAGGAGACCTTCTGCAACAGATAAGAATTGGCCTATTGTTTTAAATGTACAGTTCAGTGGGAACTACCTGTACACAAACATGGATTATCAAGAGATTTTAATCCCAAAAACTGGGATGTATAACTTGTTTTTTGTAGCATGTGATCCAAAACTAAAGGGACTAACTATGAGCGGGAAGACTTTGTGGAAAAATCCCAATGGTTATCTACCGGGTCGAATGGCTCCACTAATGAAGTTTTATGTGTTTATGGCACTTGCTTACGCGTTGCTTGGAATAATTTGGTTCACTCAGTATGTGAGGTTTTGGAAGGATATTCTGCAACTTCAGCACTGCATCACAGCTGTTATTGCTCTTGGGTTGTTTGAGATGATTCTTTGGTATTTTGATCATGTAAATTTTAATAATACAGGGATGAGGCCAATTGTTGTTACAACATTGGTTGTGACAGTTGGAGCTGTGAGAAAAACAGTTTCCCGTCTTCTTATCCTCATTGTTTCAATGGGTTATGGTGTGGTGCGGCCCACTCTTGGGGGCCTTACGTCAAAGGTGCTTCTCCTTGGAATAACATTTTTTCTGGCCACAGAGTTGCTTAATATCACGGAGTATGTGGGGACAATCAATGATGTATCCGGAAGAGCAAGATTGTTTCTAGTTCTTCCTGATGCTTTCTTGGACGCATTTTTGATATTATGGATTTTTACTTCTCTCTCAAAAACACTAGAGCAGCTACAGGCAAAGAGAAGCTCCGTCAAACTGGATATGTATAGGAAGTTCTCAAATGCATTAGCAGTCACAGTCATTGCCTCGGTTGCATGGATAGTGTATGAGGTGTACTTTAAAGCAACAGATCCATTCAATGAGAAGTGGCAGAGTGCTTGGGTCATCACTGCTTTCTGGGATATTCTTGCGTTTGCATTGCTGTGTGTGATCTGCTATCTTTGGGCTCCATCTCAGAGTTCTCAACGGTATGCTTACTCAGATGAAGTGGGAGAAGAGTCTGATGACGAAGAATCTCACTCTCTTACAAAGGCCAAGCTAGCGGGTGATATCAGCTTAGTCAAGCAAGACAACATTGAAAGCACCGAAGATTTTGATGAAGATGATACAGAAGACAAGAAGGAATGA

Protein Analysis

522

Amino Acids

58.79

Weight (kDa)

6.36

Isoelectric Point (pI)

41.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GOST_TM PF06814 222 - 468 1.5e-90 GOST, seven transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011281)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01070 AT2G01070
fragaria_vesca FvH4_5g30110 FvH4_5g30110
malus_domestica MD08G1225000.v1.1 MD15G1418500.v1.1
prunus_persica Prupe.1G562600_v2.0.a1
pyrus_communis pycom08g19610 pycom08g19620 pycom15g37150
rosa_chinensis RchiOBHm_Chr7g0224311 RchiOBHm_Chr7g0224371
rosa_laevigata RLG00000001905
rosa_multiflora Rmu_sc0025639.1_g000001
rosa_roxburghii Rroxscaffold_3G00234870
rosa_rugosa Rorug07G0222600
rosa_samantha Rh7AG380800 Rh7BG358500 Rh7DG367600
rosa_wichuraiana Rw7G031110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 323
AccB7I CCANNNNNTGG 1 cut(s) 551
AccBSI CCGCTC 1 cut(s) 612
AccI GTMKAC 1 cut(s) 138
AccII CGCG 1 cut(s) 704
AccIII TCCGGA 1 cut(s) 1070
AciI CCGC 7 cut(s) 189, 207, 219, 372, 612, 958, 1481
AclWI GGATC 3 cut(s) 124, 578, 1283
AcoI YGGCCR 1 cut(s) 1017
AcsI RAATTY 1 cut(s) 835
AcuI CTGAAG 2 cut(s) 139, 749
AfaI GTAC 4 cut(s) 304, 489, 512, 1274
AfiI CCNNNNNNNGG 4 cut(s) 258, 551, 968, 969
AflIII ACRYGT 1 cut(s) 702
AgsI TTSAA 3 cut(s) 939, 1297, 1517
AjiI CACGTC 1 cut(s) 136
AjnI CCWGG 1 cut(s) 15
AluBI AGCT 9 cut(s) 283, 338, 383, 782, 896, 1168, 1185, 1477, 1494
AluI AGCT 9 cut(s) 283, 338, 383, 782, 896, 1168, 1185, 1477, 1494
Alw26I GTCTC 3 cut(s) 87, 326, 439
AlwI GGATC 3 cut(s) 124, 578, 1283
Aor13HI TCCGGA 1 cut(s) 1070
AoxI GGCC 6 cut(s) 470, 857, 959, 973, 1017, 1470
ApeKI GCWGC 3 cut(s) 192, 272, 1165
ApoI RAATTY 1 cut(s) 835
AspS9I GGNCC 2 cut(s) 960, 973
AsuC2I CCSGG 1 cut(s) 651
AsuHPI GGTGA 3 cut(s) 241, 443, 1496
AsuNHI GCTAGC 1 cut(s) 1477
BalI TGGCCA 1 cut(s) 1019
BanII GRGCYC 1 cut(s) 1387
BbsI GAAGAC 3 cut(s) 623, 911, 1557
BbvI GCAGC 3 cut(s) 204, 259, 1177
BccI CCATC 2 cut(s) 406, 1396
BceAI ACGGC 1 cut(s) 67
BciT130I CCWGG 1 cut(s) 17
BciVI GTATCC 1 cut(s) 1078
BclI TGATCA 1 cut(s) 826
BcnI CCSGG 1 cut(s) 651
BcoDI GTCTC 3 cut(s) 87, 326, 439
BfaI CTAG 3 cut(s) 1091, 1160, 1478
BfmI CTRYAG 1 cut(s) 1169
BfuI GTATCC 1 cut(s) 1078
BisI GCNGC 6 cut(s) 190, 193, 207, 273, 959, 1166
BlsI GCNGC 6 cut(s) 191, 194, 208, 274, 960, 1167
BmcAI AGTACT 1 cut(s) 304
Bme1390I CCNGG 2 cut(s) 17, 651
BmgBI CACGTC 1 cut(s) 136
BmgT120I GGNCC 2 cut(s) 960, 973
BmiI GGNNCC 3 cut(s) 663, 974, 1386
BmrFI CCNGG 2 cut(s) 17, 651
BmrI ACTGGG 2 cut(s) 315, 561
BmsI GCATC 5 cut(s) 66, 210, 259, 783, 1093
BmtI GCTAGC 1 cut(s) 1481
BmuI ACTGGG 2 cut(s) 315, 561
BpiI GAAGAC 3 cut(s) 623, 911, 1557
BpmI CTGGAG 1 cut(s) 277
BpuMI CCSGG 1 cut(s) 651
BsaBI GATNNNNATC 1 cut(s) 539
BsaI GGTCTC 1 cut(s) 439
BsaJI CCNNGG 3 cut(s) 61, 997, 1245
BsaWI WCCGGW 1 cut(s) 1070
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bsc4I CCNNNNNNNGG 4 cut(s) 258, 551, 968, 969
Bse1I ACTGG 3 cut(s) 310, 556, 1200
Bse3DI GCAATG 2 cut(s) 1239, 1355
Bse8I GATNNNNATC 1 cut(s) 539
BseAI TCCGGA 1 cut(s) 1070
BseBI CCWGG 1 cut(s) 17
BseDI CCNNGG 3 cut(s) 61, 997, 1245
BseGI GGATG 3 cut(s) 274, 561, 858
BseJI GATNNNNATC 1 cut(s) 539
BseLI CCNNNNNNNGG 4 cut(s) 258, 551, 968, 969
BseMI GCAATG 2 cut(s) 1239, 1355
BseMII CTCAG 4 cut(s) 267, 743, 1406, 1431
BseNI ACTGG 3 cut(s) 310, 556, 1200
BseRI GAGGAG 3 cut(s) 46, 80, 274
BseXI GCAGC 3 cut(s) 204, 259, 1177
Bsh1236I CGCG 1 cut(s) 704
BshFI GGCC 6 cut(s) 472, 859, 961, 975, 1019, 1472
BsiSI CCGG 3 cut(s) 186, 650, 1071
BslFI GGGAC 2 cut(s) 612, 1066
BslI CCNNNNNNNGG 4 cut(s) 258, 551, 968, 969
BsmAI GTCTC 3 cut(s) 87, 326, 439
BsmFI GGGAC 2 cut(s) 612, 1066
BsmI GAATGC 1 cut(s) 54
BsnI GGCC 6 cut(s) 472, 859, 961, 975, 1019, 1472
Bso31I GGTCTC 1 cut(s) 439
Bsp1286I GDGCHC 1 cut(s) 1387
Bsp13I TCCGGA 1 cut(s) 1070
Bsp1407I TGTACA 2 cut(s) 487, 510
Bsp143I GATC 7 cut(s) 129, 340, 400, 583, 826, 1288, 1368
Bsp19I CCATGG 1 cut(s) 61
BspACI CCGC 7 cut(s) 189, 207, 219, 372, 612, 958, 1481
BspANI GGCC 6 cut(s) 472, 859, 961, 975, 1019, 1472
BspCNI CTCAG 4 cut(s) 268, 742, 1405, 1430
BspEI TCCGGA 1 cut(s) 1070
BspFNI CGCG 1 cut(s) 704
BspLI GGNNCC 3 cut(s) 663, 974, 1386
BspOI GCTAGC 1 cut(s) 1481
BspPI GGATC 3 cut(s) 124, 578, 1283
BspQI GCTCTTC 1 cut(s) 1069
BspTNI GGTCTC 1 cut(s) 439
BsrBI CCGCTC 1 cut(s) 612
BsrDI GCAATG 2 cut(s) 1239, 1355
BsrGI TGTACA 2 cut(s) 487, 510
BsrI ACTGG 3 cut(s) 310, 556, 1200
BssECI CCNNGG 3 cut(s) 61, 997, 1245
BssMI GATC 7 cut(s) 129, 340, 400, 583, 826, 1288, 1368
BssT1I CCWWGG 2 cut(s) 61, 997
Bst2UI CCWGG 1 cut(s) 17
Bst4CI ACNGT 5 cut(s) 492, 889, 910, 1237, 1407
Bst6I CTCTTC 2 cut(s) 1069, 1428
BstAUI TGTACA 2 cut(s) 487, 510
BstC8I GCNNGC 1 cut(s) 1479
BstDEI CTNAG 6 cut(s) 276, 408, 729, 1392, 1417, 1495
BstDSI CCRYGG 1 cut(s) 61
BstENI CCTNNNNNAGG 1 cut(s) 256
BstF5I GGATG 3 cut(s) 274, 561, 858
BstFNI CGCG 1 cut(s) 704
BstKTI GATC 7 cut(s) 132, 343, 403, 586, 829, 1291, 1371
BstMAI GTCTC 3 cut(s) 87, 326, 439
BstMBI GATC 7 cut(s) 129, 340, 400, 583, 826, 1288, 1368
BstMWI GCNNNNNNNGC 7 cut(s) 85, 94, 198, 766, 788, 1250, 1478
BstNI CCWGG 1 cut(s) 17
BstNSI RCATGY 1 cut(s) 582
BstSCI CCNGG 2 cut(s) 15, 649
BstSFI CTRYAG 1 cut(s) 1169
BstUI CGCG 1 cut(s) 704
BstV1I GCAGC 3 cut(s) 204, 259, 1177
BstV2I GAAGAC 3 cut(s) 623, 911, 1557
BstX2I RGATCY 1 cut(s) 1288
BstYI RGATCY 1 cut(s) 1288
BsuI GTATCC 1 cut(s) 1078
BsuRI GGCC 6 cut(s) 472, 859, 961, 975, 1019, 1472
BtgI CCRYGG 1 cut(s) 61
BtgZI GCGATG 1 cut(s) 187
BtrI CACGTC 1 cut(s) 136
BtsCI GGATG 3 cut(s) 274, 561, 858
BtsI GCAGTG 2 cut(s) 769, 1326
BtsIMutI CAGTG 3 cut(s) 502, 769, 1326
Cac8I GCNNGC 1 cut(s) 1479
Cfr13I GGNCC 2 cut(s) 960, 973
CseI GACGC 1 cut(s) 1124
Csp6I GTAC 4 cut(s) 303, 488, 511, 1273
CviAII CATG 6 cut(s) 62, 292, 520, 579, 830, 1254
CviQI GTAC 4 cut(s) 303, 488, 511, 1273
DdeI CTNAG 6 cut(s) 276, 408, 729, 1392, 1417, 1495
DpnI GATC 7 cut(s) 131, 342, 402, 585, 828, 1290, 1370
DpnII GATC 7 cut(s) 129, 340, 400, 583, 826, 1288, 1368
DraI TTTAAA 2 cut(s) 483, 1279
EaeI YGGCCR 1 cut(s) 1017
Eam1104I CTCTTC 2 cut(s) 1069, 1428
EarI CTCTTC 2 cut(s) 1069, 1428
EciI GGCGGA 2 cut(s) 208, 387
Eco130I CCWWGG 2 cut(s) 61, 997
Eco24I GRGCYC 1 cut(s) 1387
Eco31I GGTCTC 1 cut(s) 439
Eco32I GATATC 1 cut(s) 1489
Eco57I CTGAAG 2 cut(s) 139, 749
EcoNI CCTNNNNNAGG 1 cut(s) 256
EcoO109I RGGNCCY 1 cut(s) 973
EcoRII CCWGG 1 cut(s) 15
EcoRV GATATC 1 cut(s) 1489
EcoT14I CCWWGG 2 cut(s) 61, 997
EcoT22I ATGCAT 1 cut(s) 1225
EcoT38I GRGCYC 1 cut(s) 1387
ErhI CCWWGG 2 cut(s) 61, 997
FaeI CATG 6 cut(s) 65, 295, 523, 582, 833, 1257
FaqI GGGAC 2 cut(s) 612, 1066
FatI CATG 6 cut(s) 61, 291, 519, 578, 829, 1253
FauI CCCGC 2 cut(s) 605, 1474
FbaI TGATCA 1 cut(s) 826
FblI GTMKAC 1 cut(s) 138
Fnu4HI GCNGC 6 cut(s) 190, 193, 207, 273, 959, 1166
FokI GGATG 3 cut(s) 281, 568, 865
FriOI GRGCYC 1 cut(s) 1387
Fsp4HI GCNGC 6 cut(s) 190, 193, 207, 273, 959, 1166
FspBI CTAG 3 cut(s) 1091, 1160, 1478
GluI GCNGC 6 cut(s) 190, 193, 207, 273, 959, 1166
GsuI CTGGAG 1 cut(s) 277
HaeIII GGCC 6 cut(s) 472, 859, 961, 975, 1019, 1472
HapII CCGG 3 cut(s) 186, 650, 1071
HgaI GACGC 1 cut(s) 1124
Hin1II CATG 6 cut(s) 65, 295, 523, 582, 833, 1257
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 4 cut(s) 237, 811, 1436, 1451
HpaII CCGG 3 cut(s) 186, 650, 1071
HphI GGTGA 3 cut(s) 241, 443, 1496
Hpy166II GTNNAC 4 cut(s) 139, 513, 726, 1273
Hpy188I TCNGA 6 cut(s) 158, 289, 345, 1395, 1420, 1441
Hpy188III TCNNGA 8 cut(s) 73, 241, 256, 398, 442, 530, 1071, 1100
Hpy8I GTNNAC 4 cut(s) 139, 513, 726, 1273
HpyAV CCTTC 9 cut(s) 116, 163, 188, 191, 262, 410, 459, 742, 1552
HpyCH4III ACNGT 5 cut(s) 492, 889, 910, 1237, 1407
HpyCH4IV ACGT 2 cut(s) 135, 980
HpyCH4V TGCA 7 cut(s) 393, 455, 760, 774, 1223, 1253, 1355
HpyF10VI GCNNNNNNNGC 7 cut(s) 85, 94, 198, 766, 788, 1250, 1478
HpyF3I CTNAG 6 cut(s) 276, 408, 729, 1392, 1417, 1495
HpySE526I ACGT 2 cut(s) 135, 980
Hsp92II CATG 6 cut(s) 65, 295, 523, 582, 833, 1257
Kpn2I TCCGGA 1 cut(s) 1070
Ksp22I TGATCA 1 cut(s) 826
Kzo9I GATC 7 cut(s) 129, 340, 400, 583, 826, 1288, 1368
LguI GCTCTTC 1 cut(s) 1069
LmnI GCTCC 7 cut(s) 93, 261, 295, 667, 893, 1190, 1390
Lsp1109I GCAGC 3 cut(s) 204, 259, 1177
LweI GCATC 5 cut(s) 66, 210, 259, 783, 1093
MaeI CTAG 3 cut(s) 1091, 1160, 1478
MaeII ACGT 2 cut(s) 135, 980
MaeIII GTNAC 3 cut(s) 868, 883, 1231
MalI GATC 7 cut(s) 131, 342, 402, 585, 828, 1290, 1370
MbiI CCGCTC 1 cut(s) 612
MboI GATC 7 cut(s) 129, 340, 400, 583, 826, 1288, 1368
MfeI CAATTG 1 cut(s) 861
MflI RGATCY 1 cut(s) 1288
MhlI GDGCHC 1 cut(s) 1387
MlsI TGGCCA 1 cut(s) 1019
MluCI AATT 4 cut(s) 466, 717, 835, 861
MluI ACGCGT 1 cut(s) 702
MluNI TGGCCA 1 cut(s) 1019
MlyI GAGTC 1 cut(s) 1445
MmeI TCCRAC 1 cut(s) 871
MnlI CCTC 9 cut(s) 24, 101, 136, 252, 732, 849, 938, 1255, 1261
Mox20I TGGCCA 1 cut(s) 1019
Mph1103I ATGCAT 1 cut(s) 1225
MroI TCCGGA 1 cut(s) 1070
MscI TGGCCA 1 cut(s) 1019
MseI TTAA 5 cut(s) 482, 539, 840, 1032, 1278
MslI CAYNNNNRTG 2 cut(s) 518, 1360
Msp20I TGGCCA 1 cut(s) 1019
MspA1I CMGCKG 2 cut(s) 338, 782
MspI CCGG 3 cut(s) 186, 650, 1071
MspR9I CCNGG 2 cut(s) 17, 651
MunI CAATTG 1 cut(s) 861
Mva1269I GAATGC 1 cut(s) 54
MvaI CCWGG 1 cut(s) 17
MvnI CGCG 1 cut(s) 704
MwoI GCNNNNNNNGC 7 cut(s) 85, 94, 198, 766, 788, 1250, 1478
NciI CCSGG 1 cut(s) 651
NcoI CCATGG 1 cut(s) 61
NdeII GATC 7 cut(s) 129, 340, 400, 583, 826, 1288, 1368
NheI GCTAGC 1 cut(s) 1477
NlaIII CATG 6 cut(s) 65, 295, 523, 582, 833, 1257
NlaIV GGNNCC 3 cut(s) 663, 974, 1386
NmuCI GTSAC 2 cut(s) 883, 1231
NsiI ATGCAT 1 cut(s) 1225
NspI RCATGY 1 cut(s) 582
PciSI GCTCTTC 1 cut(s) 1069
PctI GAATGC 1 cut(s) 54
PfeI GAWTC 3 cut(s) 237, 811, 1451
PflMI CCANNNNNTGG 1 cut(s) 551
PkrI GCNGC 6 cut(s) 191, 194, 208, 274, 960, 1167
PleI GAGTC 1 cut(s) 1444
PpsI GAGTC 1 cut(s) 1444
PsiI TTATAA 1 cut(s) 323
Psp6I CCWGG 1 cut(s) 15
PspGI CCWGG 1 cut(s) 15
PspN4I GGNNCC 3 cut(s) 663, 974, 1386
PspPI GGNCC 2 cut(s) 960, 973
PsrI GAACNNNNNNTAC 4 cut(s) 494, 526, 1196, 1228
PsuI RGATCY 1 cut(s) 1288
PvuII CAGCTG 2 cut(s) 338, 782
RsaI GTAC 4 cut(s) 304, 489, 512, 1274
RsaNI GTAC 4 cut(s) 303, 488, 511, 1273
RseI CAYNNNNRTG 2 cut(s) 518, 1360
SapI GCTCTTC 1 cut(s) 1069
SaqAI TTAA 5 cut(s) 482, 539, 840, 1032, 1278
SatI GCNGC 6 cut(s) 190, 193, 207, 273, 959, 1166
Sau3AI GATC 7 cut(s) 129, 340, 400, 583, 826, 1288, 1368
Sau96I GGNCC 2 cut(s) 960, 973
ScaI AGTACT 1 cut(s) 304
SchI GAGTC 1 cut(s) 1445
ScrFI CCNGG 2 cut(s) 17, 651
SduI GDGCHC 1 cut(s) 1387
SfaNI GCATC 5 cut(s) 66, 210, 259, 783, 1093
SfcI CTRYAG 1 cut(s) 1169
SmiMI CAYNNNNRTG 2 cut(s) 518, 1360
Sse9I AATT 4 cut(s) 466, 717, 835, 861
SsiI CCGC 7 cut(s) 189, 207, 219, 372, 612, 958, 1481
SspI AATATT 1 cut(s) 327
SspMI CTAG 3 cut(s) 1091, 1160, 1478
StyD4I CCNGG 2 cut(s) 15, 649
StyI CCWWGG 2 cut(s) 61, 997
TaaI ACNGT 5 cut(s) 492, 889, 910, 1237, 1407
TaiI ACGT 2 cut(s) 138, 983
TaqI TCGA 4 cut(s) 128, 235, 240, 655
TasI AATT 4 cut(s) 466, 717, 835, 861
TatI WGTACW 4 cut(s) 302, 487, 510, 1272
TauI GCSGC 3 cut(s) 192, 209, 961
TfiI GAWTC 3 cut(s) 237, 811, 1451
Tru1I TTAA 5 cut(s) 482, 539, 840, 1032, 1278
Tru9I TTAA 5 cut(s) 482, 539, 840, 1032, 1278
TscAI CASTG 3 cut(s) 502, 776, 1333
TseFI GTSAC 2 cut(s) 883, 1231
TseI GCWGC 3 cut(s) 192, 272, 1165
Tsp45I GTSAC 2 cut(s) 883, 1231
TspDTI ATGAA 3 cut(s) 686, 1437, 1551
TspGWI ACGGA 2 cut(s) 1055, 1177
TspRI CASTG 3 cut(s) 502, 776, 1333
Van91I CCANNNNNTGG 1 cut(s) 551
XagI CCTNNNNNAGG 1 cut(s) 256
XapI RAATTY 1 cut(s) 835
XceI RCATGY 1 cut(s) 582
XmiI GTMKAC 1 cut(s) 138
XspI CTAG 3 cut(s) 1091, 1160, 1478
ZrmI AGTACT 1 cut(s) 304
Zsp2I ATGCAT 1 cut(s) 1225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.