RLG00000001905

Transmembrane protein 87B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0011281
Physical Location & Seq
Reverse (-)
22805236 .. 22808554
3319 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001905

Sequence Viewer

Length: 1554 bp
ATGGATTTCACATTCCAGAGAAGAGGAAGAGGAAGAAGGAGAAGACTCACCGACCTCTCGCTGCTCCTCATCCTCCTCCTCCTTTTAACATTATTCAATTCCACTCCGGCCAACGCCTCCATCCATATCTACCGCAATGAAATCTTCCGGGAAGTCGGCAATGCTTTCCTCCTCTCCGGCGGCAGCGAAGGCATCTCCGCCTCTCCCTCCTCCCGCTCTTTTATCCGATTCGAGAATATCACCTTCTGGAGGAGCGAGGCTGCTGTTGACAAGAAGCATAGTAATGGGTTGATACAGGTTATTATTTTTGAAGCCGCGGATCGTAATAATATCGGTGGTTCTGCTTATGGGGGACAGAGATCTATATGTTGCACCCCTGATCTTGCTAAGATGGAAGGTTGTAAGCAAGGTGAAGTCATTAGGAGACCTTCTGCCACAGATGTTAATTGGCCCATTGTTATAAATGTACACTTCAGAAGGAACAGTCTCTCTAAAAATATGGATTATAAAGAGATTTTGATCACGAAAACGGGGATGTATAACTTGTTTTTTGTAGCCTGTGATCCAAAGCTGAAGGAACTAGTAATGAGCGGGAAGACGATATGGAAAAGTCCCGATGGTTATCTACCTGGTCGAATGGCTCCACTGATGAAATTTTACGTGTATATGGCACTTGCTTATGTGCTGATTGGTGTAATTTGGCTCACTCAGTATGTGAGGTTCTGGAAGGATATTCTGCAACTTCAGCATTGCATCACGGCTGTCATTGCTCTTGGATTGTTTGAGATGATTCTTTGGTATTCCGACTATGTCCATTTTAATAATACAGGGATGAGGCCAGTTTTTCTTACGACATTGGTTGTGACAGTTGGAGCTGTGAGAAAAACAGTTTCCCGTCTCCTTATCCTCACTGTTTCCATGGGCTATGGTGTAGTACGGCCTACTCTTGGGGGTCTTACCTCCAAGGTGCTTCTCCTTGGAATAACATTTTTTCTGGCAACAGAGTTGCTTAATATTACCGAGTACGTTGGGACAATTAATGATGTATCAGGAAGAGCGAGATTATTTCTAGTTCTTCCTGATGCTTTCTTGGACGCATTTTTAATCTTGTGGATTTTTACCTCTCTCTCCAAAACACTAGAACAGCTACAGGCAAAGAGAAGCTCTGTCAAGTTGGATATGTATAGGAAGTTCTCAAATGCATTAGCAGTGACAGTCATTGTCTCAGTTGCATGGATAGCTTATGAGGTGTACTTCAAAGCAACAGATCCCTTCAATGAGAAGTGGCATAGTGCTTGGATCATCACTGCTTTCTGGGATATTCTTGCATTTGCATTGCTGAGCGTTATCTGCTATCTTTGGGCACCATCTCAGAACTCTCAGAGGTATGCATACTCAGAGAAGCTAGGAGAAGAATCTGACGATGAAGAATCTCAGTCTCTAACTGGGGGCAAGCCGGAGGGTGATATCAGCTTAGTCAATCAAGAAGAGAAGAGTGGTGGAAGCACCGGAGATTCTGATGAAGAGGACACAGAAGAAGATAAGAGGGAATGA

Protein Analysis

518

Amino Acids

58.37

Weight (kDa)

8.07

Isoelectric Point (pI)

49.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GOST_TM PF06814 215 - 461 8.6e-89 GOST, seven transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011281)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01070 AT2G01070
fragaria_vesca FvH4_5g30110 FvH4_5g30110
malus_domestica MD08G1225000.v1.1 MD15G1418500.v1.1
prunus_persica Prupe.1G562600_v2.0.a1
pyrus_communis pycom08g19610 pycom08g19620 pycom15g37150
rosa_chinensis RchiOBHm_Chr7g0224311 RchiOBHm_Chr7g0224371
rosa_laevigata RLG00000001905
rosa_multiflora Rmu_sc0025639.1_g000001
rosa_roxburghii Rroxscaffold_3G00234870
rosa_rugosa Rorug07G0222600
rosa_samantha Rh7AG380800 Rh7BG358500 Rh7DG367600
rosa_wichuraiana Rw7G031110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 461, 507
AccB1I GGYRCC 1 cut(s) 1363
AccBSI CCGCTC 2 cut(s) 216, 591
AccII CGCG 1 cut(s) 317
AciI CCGC 7 cut(s) 133, 180, 198, 214, 315, 317, 591
AclWI GGATC 4 cut(s) 327, 557, 1262, 1307
AcoI YGGCCR 1 cut(s) 108
AcsI RAATTY 1 cut(s) 653
AcuI CTGAAG 3 cut(s) 457, 593, 728
AfaI GTAC 4 cut(s) 468, 936, 1025, 1253
AfiI CCNNNNNNNGG 2 cut(s) 249, 947
AflIII ACRYGT 1 cut(s) 660
AgsI TTSAA 4 cut(s) 97, 311, 1258, 1276
AhlI ACTAGT 1 cut(s) 580
AjnI CCWGG 1 cut(s) 628
AluBI AGCT 7 cut(s) 571, 875, 1147, 1164, 1241, 1405, 1473
AluI AGCT 7 cut(s) 571, 875, 1147, 1164, 1241, 1405, 1473
Alw26I GTCTC 5 cut(s) 418, 491, 902, 1228, 1443
AlwI GGATC 4 cut(s) 327, 557, 1262, 1307
AoxI GGCC 4 cut(s) 108, 449, 836, 938
ApeKI GCWGC 3 cut(s) 61, 183, 260
ApoI RAATTY 1 cut(s) 653
AseI ATTAAT 1 cut(s) 1038
AspS9I GGNCC 1 cut(s) 450
AsuC2I CCSGG 1 cut(s) 149
AsuHPI GGTGA 4 cut(s) 40, 232, 422, 1475
BaeGI GKGCMC 1 cut(s) 1366
BanI GGYRCC 1 cut(s) 1363
BbsI GAAGAC 2 cut(s) 49, 602
BbvI GCAGC 3 cut(s) 48, 195, 247
BccI CCATC 4 cut(s) 128, 385, 611, 1375
BceAI ACGGC 2 cut(s) 774, 953
BciT130I CCWGG 1 cut(s) 630
BclI TGATCA 1 cut(s) 519
BcnI CCSGG 1 cut(s) 149
BcoDI GTCTC 5 cut(s) 418, 491, 902, 1228, 1443
BcuI ACTAGT 1 cut(s) 580
BfaI CTAG 4 cut(s) 581, 1070, 1139, 1406
BfmI CTRYAG 1 cut(s) 1148
BglII AGATCT 1 cut(s) 359
BisI GCNGC 5 cut(s) 62, 181, 184, 261, 315
BlpI GCTNAGC 1 cut(s) 1340
BlsI GCNGC 5 cut(s) 63, 182, 185, 262, 316
Bme1390I CCNGG 2 cut(s) 149, 630
BmgT120I GGNCC 1 cut(s) 450
BmiI GGNNCC 2 cut(s) 642, 1365
BmrFI CCNGG 2 cut(s) 149, 630
BmrI ACTGGG 1 cut(s) 1455
BmsI GCATC 3 cut(s) 201, 762, 1072
BmuI ACTGGG 1 cut(s) 1455
BpiI GAAGAC 2 cut(s) 49, 602
BpmI CTGGAG 1 cut(s) 268
Bpu1102I GCTNAGC 1 cut(s) 1340
BpuMI CCSGG 1 cut(s) 149
BsaAI YACGTR 1 cut(s) 661
BsaBI GATNNNNATC 2 cut(s) 518, 621
BsaI GGTCTC 1 cut(s) 418
BsaJI CCNNGG 4 cut(s) 315, 918, 963, 976
BsaWI WCCGGW 1 cut(s) 1508
Bsc4I CCNNNNNNNGG 2 cut(s) 249, 947
Bse1I ACTGG 2 cut(s) 839, 1450
Bse3DI GCAATG 5 cut(s) 142, 166, 748, 765, 1334
Bse8I GATNNNNATC 2 cut(s) 518, 621
BseBI CCWGG 1 cut(s) 630
BseDI CCNNGG 4 cut(s) 315, 918, 963, 976
BseGI GGATG 4 cut(s) 69, 120, 540, 837
BseJI GATNNNNATC 2 cut(s) 518, 621
BseLI CCNNNNNNNGG 2 cut(s) 249, 947
BseMI GCAATG 5 cut(s) 142, 166, 748, 765, 1334
BseMII CTCAG 7 cut(s) 722, 1239, 1331, 1385, 1394, 1410, 1448
BseNI ACTGG 2 cut(s) 839, 1450
BseRI GAGGAG 6 cut(s) 56, 65, 68, 161, 199, 265
BseSI GKGCMC 1 cut(s) 1366
BseXI GCAGC 3 cut(s) 48, 195, 247
Bsh1236I CGCG 1 cut(s) 317
BshFI GGCC 4 cut(s) 110, 451, 838, 940
BshNI GGYRCC 1 cut(s) 1363
BsiSI CCGG 5 cut(s) 107, 148, 177, 1457, 1509
BslFI GGGAC 3 cut(s) 366, 597, 1045
BslI CCNNNNNNNGG 2 cut(s) 249, 947
BsmAI GTCTC 5 cut(s) 418, 491, 902, 1228, 1443
BsmBI CGTCTC 1 cut(s) 902
BsmFI GGGAC 3 cut(s) 366, 597, 1045
BsnI GGCC 4 cut(s) 110, 451, 838, 940
Bso31I GGTCTC 1 cut(s) 418
Bsp1286I GDGCHC 1 cut(s) 1366
Bsp1407I TGTACA 1 cut(s) 466
Bsp143I GATC 7 cut(s) 319, 359, 379, 519, 562, 1267, 1299
Bsp1720I GCTNAGC 1 cut(s) 1340
Bsp19I CCATGG 1 cut(s) 918
BspACI CCGC 7 cut(s) 133, 180, 198, 214, 315, 317, 591
BspANI GGCC 4 cut(s) 110, 451, 838, 940
BspCNI CTCAG 7 cut(s) 721, 1238, 1332, 1384, 1393, 1409, 1447
BspFNI CGCG 1 cut(s) 317
BspLI GGNNCC 2 cut(s) 642, 1365
BspPI GGATC 4 cut(s) 327, 557, 1262, 1307
BspQI GCTCTTC 1 cut(s) 1048
BspT107I GGYRCC 1 cut(s) 1363
BspTNI GGTCTC 1 cut(s) 418
BsrBI CCGCTC 2 cut(s) 216, 591
BsrDI GCAATG 5 cut(s) 142, 166, 748, 765, 1334
BsrGI TGTACA 1 cut(s) 466
BsrI ACTGG 2 cut(s) 839, 1450
BssECI CCNNGG 4 cut(s) 315, 918, 963, 976
BssMI GATC 7 cut(s) 319, 359, 379, 519, 562, 1267, 1299
BssT1I CCWWGG 3 cut(s) 918, 963, 976
Bst2UI CCWGG 1 cut(s) 630
Bst4CI ACNGT 5 cut(s) 485, 868, 889, 913, 1216
Bst6I CTCTTC 6 cut(s) 16, 22, 1048, 1482, 1487, 1518
BstAUI TGTACA 1 cut(s) 466
BstBAI YACGTR 1 cut(s) 661
BstC8I GCNNGC 1 cut(s) 1454
BstDEI CTNAG 9 cut(s) 387, 708, 1225, 1340, 1371, 1380, 1396, 1434, 1474
BstDSI CCRYGG 2 cut(s) 315, 918
BstENI CCTNNNNNAGG 1 cut(s) 247
BstF5I GGATG 4 cut(s) 69, 120, 540, 837
BstFNI CGCG 1 cut(s) 317
BstKTI GATC 7 cut(s) 322, 362, 382, 522, 565, 1270, 1302
BstMAI GTCTC 5 cut(s) 418, 491, 902, 1228, 1443
BstMBI GATC 7 cut(s) 319, 359, 379, 519, 562, 1267, 1299
BstMWI GCNNNNNNNGC 5 cut(s) 189, 745, 767, 1238, 1350
BstNI CCWGG 1 cut(s) 630
BstSCI CCNGG 2 cut(s) 147, 628
BstSFI CTRYAG 1 cut(s) 1148
BstSLI GKGCMC 1 cut(s) 1366
BstUI CGCG 1 cut(s) 317
BstV1I GCAGC 3 cut(s) 48, 195, 247
BstV2I GAAGAC 2 cut(s) 49, 602
BstX2I RGATCY 2 cut(s) 359, 1267
BstYI RGATCY 2 cut(s) 359, 1267
BsuRI GGCC 4 cut(s) 110, 451, 838, 940
BtgI CCRYGG 2 cut(s) 315, 918
BtsCI GGATG 4 cut(s) 69, 120, 540, 837
BtsI GCAGTG 2 cut(s) 1215, 1305
BtsIMutI CAGTG 4 cut(s) 644, 909, 1215, 1305
Cac8I GCNNGC 1 cut(s) 1454
Cfr13I GGNCC 1 cut(s) 450
Cfr42I CCGCGG 1 cut(s) 318
CseI GACGC 1 cut(s) 1103
CsiI ACCWGGT 1 cut(s) 628
Csp6I GTAC 4 cut(s) 467, 935, 1024, 1252
CviAII CATG 2 cut(s) 919, 1233
CviQI GTAC 4 cut(s) 467, 935, 1024, 1252
DdeI CTNAG 9 cut(s) 387, 708, 1225, 1340, 1371, 1380, 1396, 1434, 1474
DpnI GATC 7 cut(s) 321, 361, 381, 521, 564, 1269, 1301
DpnII GATC 7 cut(s) 319, 359, 379, 519, 562, 1267, 1299
EaeI YGGCCR 1 cut(s) 108
Eam1104I CTCTTC 6 cut(s) 16, 22, 1048, 1482, 1487, 1518
EarI CTCTTC 6 cut(s) 16, 22, 1048, 1482, 1487, 1518
EciI GGCGGA 1 cut(s) 187
Eco130I CCWWGG 3 cut(s) 918, 963, 976
Eco31I GGTCTC 1 cut(s) 418
Eco32I GATATC 1 cut(s) 1468
Eco57I CTGAAG 3 cut(s) 457, 593, 728
EcoNI CCTNNNNNAGG 1 cut(s) 247
EcoRII CCWGG 1 cut(s) 628
EcoRV GATATC 1 cut(s) 1468
EcoT14I CCWWGG 3 cut(s) 918, 963, 976
EcoT22I ATGCAT 2 cut(s) 1204, 1393
ErhI CCWWGG 3 cut(s) 918, 963, 976
Esp3I CGTCTC 1 cut(s) 902
FaeI CATG 2 cut(s) 922, 1236
FaqI GGGAC 3 cut(s) 366, 597, 1045
FatI CATG 2 cut(s) 918, 1232
FauI CCCGC 2 cut(s) 221, 584
FbaI TGATCA 1 cut(s) 519
Fnu4HI GCNGC 5 cut(s) 62, 181, 184, 261, 315
FokI GGATG 4 cut(s) 56, 107, 547, 844
Fsp4HI GCNGC 5 cut(s) 62, 181, 184, 261, 315
FspBI CTAG 4 cut(s) 581, 1070, 1139, 1406
GluI GCNGC 5 cut(s) 62, 181, 184, 261, 315
GsuI CTGGAG 1 cut(s) 268
HaeIII GGCC 4 cut(s) 110, 451, 838, 940
HapII CCGG 5 cut(s) 107, 148, 177, 1457, 1509
HgaI GACGC 1 cut(s) 1103
Hin1II CATG 2 cut(s) 922, 1236
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HinfI GANTC 6 cut(s) 45, 228, 790, 1415, 1430, 1514
HpaII CCGG 5 cut(s) 107, 148, 177, 1457, 1509
HphI GGTGA 4 cut(s) 40, 232, 422, 1475
Hpy166II GTNNAC 3 cut(s) 268, 469, 1252
Hpy188I TCNGA 8 cut(s) 227, 476, 805, 1374, 1383, 1399, 1420, 1519
Hpy188III TCNNGA 9 cut(s) 16, 232, 247, 523, 614, 724, 1050, 1079, 1484
Hpy8I GTNNAC 3 cut(s) 268, 469, 1252
HpyAV CCTTC 9 cut(s) 30, 182, 253, 389, 438, 471, 568, 721, 1282
HpyCH4III ACNGT 5 cut(s) 485, 868, 889, 913, 1216
HpyCH4IV ACGT 2 cut(s) 660, 1026
HpyCH4V TGCA 8 cut(s) 372, 739, 753, 1202, 1232, 1328, 1334, 1391
HpyF10VI GCNNNNNNNGC 5 cut(s) 189, 745, 767, 1238, 1350
HpyF3I CTNAG 9 cut(s) 387, 708, 1225, 1340, 1371, 1380, 1396, 1434, 1474
HpySE526I ACGT 2 cut(s) 660, 1026
Hsp92II CATG 2 cut(s) 922, 1236
Ksp22I TGATCA 1 cut(s) 519
KspI CCGCGG 1 cut(s) 318
Kzo9I GATC 7 cut(s) 319, 359, 379, 519, 562, 1267, 1299
LguI GCTCTTC 1 cut(s) 1048
LmnI GCTCC 4 cut(s) 69, 252, 646, 872
Lsp1109I GCAGC 3 cut(s) 48, 195, 247
LweI GCATC 3 cut(s) 201, 762, 1072
MabI ACCWGGT 1 cut(s) 628
MaeI CTAG 4 cut(s) 581, 1070, 1139, 1406
MaeII ACGT 2 cut(s) 660, 1026
MaeIII GTNAC 2 cut(s) 862, 1210
MalI GATC 7 cut(s) 321, 361, 381, 521, 564, 1269, 1301
MbiI CCGCTC 2 cut(s) 216, 591
MboI GATC 7 cut(s) 319, 359, 379, 519, 562, 1267, 1299
MflI RGATCY 2 cut(s) 359, 1267
MhlI GDGCHC 1 cut(s) 1366
MluCI AATT 5 cut(s) 97, 445, 653, 696, 1035
MlyI GAGTC 1 cut(s) 39
MmeI TCCRAC 3 cut(s) 828, 850, 1155
Mph1103I ATGCAT 2 cut(s) 1204, 1393
MseI TTAA 6 cut(s) 86, 444, 819, 1011, 1038, 1103
MslI CAYNNNNRTG 1 cut(s) 282
MspA1I CMGCKG 1 cut(s) 317
MspI CCGG 5 cut(s) 107, 148, 177, 1457, 1509
MspR9I CCNGG 2 cut(s) 149, 630
MvaI CCWGG 1 cut(s) 630
MvnI CGCG 1 cut(s) 317
MwoI GCNNNNNNNGC 5 cut(s) 189, 745, 767, 1238, 1350
NciI CCSGG 1 cut(s) 149
NcoI CCATGG 1 cut(s) 918
NdeII GATC 7 cut(s) 319, 359, 379, 519, 562, 1267, 1299
NlaIII CATG 2 cut(s) 922, 1236
NlaIV GGNNCC 2 cut(s) 642, 1365
NmuCI GTSAC 2 cut(s) 862, 1210
NsiI ATGCAT 2 cut(s) 1204, 1393
PciSI GCTCTTC 1 cut(s) 1048
PfeI GAWTC 5 cut(s) 228, 790, 1415, 1430, 1514
PflFI GACNNNGTC 1 cut(s) 809
PfoI TCCNGGA 1 cut(s) 147
PkrI GCNGC 5 cut(s) 63, 182, 185, 262, 316
PleI GAGTC 1 cut(s) 39
PpsI GAGTC 1 cut(s) 39
Ppu21I YACGTR 1 cut(s) 661
PshBI ATTAAT 1 cut(s) 1038
PsiI TTATAA 2 cut(s) 461, 507
Psp6I CCWGG 1 cut(s) 628
PspGI CCWGG 1 cut(s) 628
PspN4I GGNNCC 2 cut(s) 642, 1365
PspPI GGNCC 1 cut(s) 450
PsrI GAACNNNNNNTAC 4 cut(s) 704, 736, 1175, 1207
PsuI RGATCY 2 cut(s) 359, 1267
PsyI GACNNNGTC 1 cut(s) 809
RsaI GTAC 4 cut(s) 468, 936, 1025, 1253
RsaNI GTAC 4 cut(s) 467, 935, 1024, 1252
RseI CAYNNNNRTG 1 cut(s) 282
SacII CCGCGG 1 cut(s) 318
SapI GCTCTTC 1 cut(s) 1048
SaqAI TTAA 6 cut(s) 86, 444, 819, 1011, 1038, 1103
SatI GCNGC 5 cut(s) 62, 181, 184, 261, 315
Sau3AI GATC 7 cut(s) 319, 359, 379, 519, 562, 1267, 1299
Sau96I GGNCC 1 cut(s) 450
SchI GAGTC 1 cut(s) 39
ScrFI CCNGG 2 cut(s) 149, 630
SduI GDGCHC 1 cut(s) 1366
SexAI ACCWGGT 1 cut(s) 628
SfaNI GCATC 3 cut(s) 201, 762, 1072
SfcI CTRYAG 1 cut(s) 1148
Sfr303I CCGCGG 1 cut(s) 318
SgrBI CCGCGG 1 cut(s) 318
SmiMI CAYNNNNRTG 1 cut(s) 282
SpeI ACTAGT 1 cut(s) 580
Sse9I AATT 5 cut(s) 97, 445, 653, 696, 1035
SsiI CCGC 7 cut(s) 133, 180, 198, 214, 315, 317, 591
SspI AATATT 1 cut(s) 1015
SspMI CTAG 4 cut(s) 581, 1070, 1139, 1406
StyD4I CCNGG 2 cut(s) 147, 628
StyI CCWWGG 3 cut(s) 918, 963, 976
TaaI ACNGT 5 cut(s) 485, 868, 889, 913, 1216
TaiI ACGT 2 cut(s) 663, 1029
TaqI TCGA 2 cut(s) 231, 634
TasI AATT 5 cut(s) 97, 445, 653, 696, 1035
TatI WGTACW 2 cut(s) 466, 1251
TauI GCSGC 2 cut(s) 183, 317
TfiI GAWTC 5 cut(s) 228, 790, 1415, 1430, 1514
Tru1I TTAA 6 cut(s) 86, 444, 819, 1011, 1038, 1103
Tru9I TTAA 6 cut(s) 86, 444, 819, 1011, 1038, 1103
TscAI CASTG 4 cut(s) 651, 916, 1215, 1312
TseFI GTSAC 2 cut(s) 862, 1210
TseI GCWGC 3 cut(s) 61, 183, 260
Tsp45I GTSAC 2 cut(s) 862, 1210
TspDTI ATGAA 4 cut(s) 153, 665, 1440, 1536
TspRI CASTG 4 cut(s) 651, 916, 1215, 1312
Tth111I GACNNNGTC 1 cut(s) 809
VspI ATTAAT 1 cut(s) 1038
XagI CCTNNNNNAGG 1 cut(s) 247
XapI RAATTY 1 cut(s) 653
XspI CTAG 4 cut(s) 581, 1070, 1139, 1406
Zsp2I ATGCAT 2 cut(s) 1204, 1393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.