MD15G1418500.v1.1

Transmembrane protein 87B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011281
Physical Location & Seq
Forward (+)
52001227 .. 52006230
5004 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1418500.v1.1.491

Sequence Viewer

Length: 1566 bp
ATGGATTTCACAACCCAGATTCGAGCGGCGAGAAGAAGAAGAAGCAACGGCATTCTCCATGGCATTTTCATGAAGCTCTCTCTGCTTCTCCTACTGTCATTTCTTCCAAATCCCACAAACGCCTCGATCCACATCTACGACAGCCAAACCTTCAGAGAAGTCGGCAATGCTTACCTTCTACCCGGCGGCAGCGAAGGCATCGCCGCCACTCTCTCCGCACGTTCTTACATTCGATTCGAGAATATCACCTTCTGGAGGAGCAAGGATGCTGCTGAGAAGCTTTCAGACATGAAGCATAGTACTGGGTTGATACAAGTTATAATATTTGAGGCAGCTGATCGTAATAATATTGGTGGTTCTGCTTATGGTGGACAAAGAGATATATGTTGCTCTCCTGATCTCGCTAAGATGGAAGGTTGTAAGCAAGGTGAAGTCATTAGGAGACCTTCTGCAACAGATAATAAATGGCCTATTGTTTTAAATGTACGGTTCAGTGGGAACTACCTGCACACAAACATGGGTTATCAAGAGATTTTAATCCCAAAAACTGGGATGTATAACTTGTTCTTTGTAGCATGTGATCCAAAACTGAAGGGAGTAACTATGAGCGGGAAGACTTTGTGGAAAAATCCTGATGGTTACCTACCTGGTCGAATGGCTCCACTAATGAAGTTTTATGTGTTTATGTCACTTGCTTACGTGTTGCTTGGAATAATTTGGTTCACTCAGTATGTGAGGTTTTGGAAGGATATTCTGCAACTTCAGCACTGCATCACAGCTGTTATTGCTCTTGGGTTGTTTGAGATGATTCTTTGGTATTGTGATTATGATAATTTTAATAGTACAGGGATGAGGCCAATTGTTGTTACAACATTGGTTGTGACGGTTGGAGCTGTAAGAAAAACAGTTTCCCGTCTTCTTATCCTCATTGTTTCCATGGGTTATGGTGTGGTGCGGCCCACTCTTGGGGGTCTTACCTCAAAGGTGATTCTCCTTGGAATAACATTTTTTCTGGCCACAGAGTTGCTTAATATTACAGAGTATGTGGGGACAATCAATGATGTATCCGGAAGAGCAAGATTATTTCTAGTTCTTCCTGATGCTTTCTTGGACGCATTTTTGATATTATGGATTTTTACCTCTCTCTCTAAAACGCTAGAGCAGCTACAGGCAAAGAGAAGCTCTGTCAAACTGGATATGTATAGGAAGTTCTCAAATGCATTAGCAGTCGCAGTCATTGCCTCAGTTGCATGGATAGTGTATGAGGTGTACTTTAAAGCAACAGATCCATTCAACGAGAAGTGGCAAAGTGCTTGGGTCATCACTGCTTTCTGGGATATTCTTGCATTTGCATTGCTGTGTGTTATCTGCTATCTTTGGGCTCCATCCCAGAGTTCTCAACGGTATGCTTACTCGGATGAAGTGGGAGAAGAATCTGACGATGAAGAATCTCAATCTCTTACGAGGGGCAAGCCAGAGGGTGATATCAGCTTAGTCAAGCAAGAGAACATTGACAACGCCAAAGATTTTGATGAAGATGATACAGAAGAAGACAAGAGGGAATGA

Protein Analysis

522

Amino Acids

58.8

Weight (kDa)

6.57

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GOST_TM PF06814 221 - 467 3.1e-92 GOST, seven transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011281)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01070 AT2G01070
fragaria_vesca FvH4_5g30110 FvH4_5g30110
malus_domestica MD08G1225000.v1.1 MD15G1418500.v1.1
prunus_persica Prupe.1G562600_v2.0.a1
pyrus_communis pycom08g19610 pycom08g19620 pycom15g37150
rosa_chinensis RchiOBHm_Chr7g0224311 RchiOBHm_Chr7g0224371
rosa_laevigata RLG00000001905
rosa_multiflora Rmu_sc0025639.1_g000001
rosa_roxburghii Rroxscaffold_3G00234870
rosa_rugosa Rorug07G0222600
rosa_samantha Rh7AG380800 Rh7BG358500 Rh7DG367600
rosa_wichuraiana Rw7G031110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 320
Acc36I ACCTGC 1 cut(s) 513
AccB7I CCANNNNNTGG 1 cut(s) 548
AccBSI CCGCTC 2 cut(s) 26, 609
AccIII TCCGGA 1 cut(s) 1067
AciI CCGC 6 cut(s) 26, 186, 204, 216, 609, 955
AclWI GGATC 3 cut(s) 121, 575, 1280
AcoI YGGCCR 1 cut(s) 1014
AcuI CTGAAG 3 cut(s) 136, 611, 746
AfaI GTAC 4 cut(s) 301, 486, 844, 1271
AfiI CCNNNNNNNGG 4 cut(s) 255, 548, 965, 966
AflIII ACRYGT 1 cut(s) 699
AgsI TTSAA 1 cut(s) 1294
AjnI CCWGG 1 cut(s) 646
AluBI AGCT 8 cut(s) 76, 280, 335, 779, 893, 1165, 1182, 1491
AluI AGCT 8 cut(s) 76, 280, 335, 779, 893, 1165, 1182, 1491
Alw26I GTCTC 1 cut(s) 436
AlwI GGATC 3 cut(s) 121, 575, 1280
Aor13HI TCCGGA 1 cut(s) 1067
AoxI GGCC 4 cut(s) 467, 854, 956, 1014
ApeKI GCWGC 4 cut(s) 189, 269, 332, 1162
AspS9I GGNCC 1 cut(s) 957
AsuC2I CCSGG 1 cut(s) 183
AsuHPI GGTGA 4 cut(s) 238, 440, 997, 1493
BalI TGGCCA 1 cut(s) 1016
BanII GRGCYC 1 cut(s) 1384
BbsI GAAGAC 3 cut(s) 620, 908, 1557
BbvI GCAGC 4 cut(s) 201, 256, 344, 1174
BccI CCATC 3 cut(s) 403, 629, 1393
BceAI ACGGC 1 cut(s) 64
BciT130I CCWGG 1 cut(s) 648
BciVI GTATCC 1 cut(s) 1075
BcnI CCSGG 1 cut(s) 183
BcoDI GTCTC 1 cut(s) 436
BfaI CTAG 2 cut(s) 1088, 1157
BfmI CTRYAG 1 cut(s) 1166
BfuAI ACCTGC 1 cut(s) 513
BfuI GTATCC 1 cut(s) 1075
BisI GCNGC 8 cut(s) 27, 187, 190, 204, 270, 333, 956, 1163
BlsI GCNGC 8 cut(s) 28, 188, 191, 205, 271, 334, 957, 1164
BmcAI AGTACT 1 cut(s) 301
Bme1390I CCNGG 2 cut(s) 183, 648
BmgT120I GGNCC 1 cut(s) 957
BmiI GGNNCC 2 cut(s) 660, 1383
BmrFI CCNGG 2 cut(s) 183, 648
BmrI ACTGGG 2 cut(s) 312, 558
BmsI GCATC 4 cut(s) 207, 256, 780, 1090
BmuI ACTGGG 2 cut(s) 312, 558
BpiI GAAGAC 3 cut(s) 620, 908, 1557
BpmI CTGGAG 1 cut(s) 274
BpuMI CCSGG 1 cut(s) 183
BsaAI YACGTR 1 cut(s) 700
BsaBI GATNNNNATC 2 cut(s) 131, 536
BsaI GGTCTC 1 cut(s) 436
BsaJI CCNNGG 3 cut(s) 58, 936, 994
BsaWI WCCGGW 1 cut(s) 1067
Bsc4I CCNNNNNNNGG 4 cut(s) 255, 548, 965, 966
Bse1I ACTGG 3 cut(s) 307, 553, 1197
Bse3DI GCAATG 3 cut(s) 172, 1236, 1352
Bse8I GATNNNNATC 2 cut(s) 131, 536
BseAI TCCGGA 1 cut(s) 1067
BseBI CCWGG 1 cut(s) 648
BseDI CCNNGG 3 cut(s) 58, 936, 994
BseGI GGATG 5 cut(s) 271, 558, 855, 1385, 1423
BseJI GATNNNNATC 2 cut(s) 131, 536
BseLI CCNNNNNNNGG 4 cut(s) 255, 548, 965, 966
BseMI GCAATG 3 cut(s) 172, 1236, 1352
BseMII CTCAG 3 cut(s) 264, 740, 1257
BseNI ACTGG 3 cut(s) 307, 553, 1197
BseRI GAGGAG 1 cut(s) 271
BseXI GCAGC 4 cut(s) 201, 256, 344, 1174
BsgI GTGCAG 1 cut(s) 491
BshFI GGCC 4 cut(s) 469, 856, 958, 1016
BsiSI CCGG 2 cut(s) 183, 1068
BslFI GGGAC 1 cut(s) 1063
BslI CCNNNNNNNGG 4 cut(s) 255, 548, 965, 966
BsmAI GTCTC 1 cut(s) 436
BsmFI GGGAC 1 cut(s) 1063
BsmI GAATGC 1 cut(s) 51
BsnI GGCC 4 cut(s) 469, 856, 958, 1016
Bso31I GGTCTC 1 cut(s) 436
Bsp1286I GDGCHC 1 cut(s) 1384
Bsp13I TCCGGA 1 cut(s) 1067
Bsp143I GATC 5 cut(s) 126, 337, 397, 580, 1285
Bsp19I CCATGG 2 cut(s) 58, 936
BspACI CCGC 6 cut(s) 26, 186, 204, 216, 609, 955
BspANI GGCC 4 cut(s) 469, 856, 958, 1016
BspCNI CTCAG 3 cut(s) 265, 739, 1256
BspEI TCCGGA 1 cut(s) 1067
BspHI TCATGA 1 cut(s) 69
BspLI GGNNCC 2 cut(s) 660, 1383
BspMI ACCTGC 1 cut(s) 513
BspPI GGATC 3 cut(s) 121, 575, 1280
BspQI GCTCTTC 1 cut(s) 1066
BspTNI GGTCTC 1 cut(s) 436
BsrBI CCGCTC 2 cut(s) 26, 609
BsrDI GCAATG 3 cut(s) 172, 1236, 1352
BsrI ACTGG 3 cut(s) 307, 553, 1197
BssECI CCNNGG 3 cut(s) 58, 936, 994
BssMI GATC 5 cut(s) 126, 337, 397, 580, 1285
BssT1I CCWWGG 3 cut(s) 58, 936, 994
Bst2UI CCWGG 1 cut(s) 648
Bst4CI ACNGT 5 cut(s) 96, 489, 886, 907, 1404
Bst6I CTCTTC 1 cut(s) 1066
BstAPI GCANNNNNTGC 1 cut(s) 1238
BstBAI YACGTR 1 cut(s) 700
BstC8I GCNNGC 1 cut(s) 1472
BstDEI CTNAG 5 cut(s) 273, 405, 726, 1243, 1492
BstDSI CCRYGG 2 cut(s) 58, 936
BstEII GGTNACC 1 cut(s) 638
BstENI CCTNNNNNAGG 1 cut(s) 253
BstF5I GGATG 5 cut(s) 271, 558, 855, 1385, 1423
BstKTI GATC 5 cut(s) 129, 340, 400, 583, 1288
BstMAI GTCTC 1 cut(s) 436
BstMBI GATC 5 cut(s) 126, 337, 397, 580, 1285
BstMWI GCNNNNNNNGC 7 cut(s) 82, 195, 763, 785, 1162, 1238, 1247
BstNI CCWGG 1 cut(s) 648
BstNSI RCATGY 1 cut(s) 579
BstPI GGTNACC 1 cut(s) 638
BstSCI CCNGG 2 cut(s) 181, 646
BstSFI CTRYAG 1 cut(s) 1166
BstV1I GCAGC 4 cut(s) 201, 256, 344, 1174
BstV2I GAAGAC 3 cut(s) 620, 908, 1557
BstX2I RGATCY 1 cut(s) 1285
BstYI RGATCY 1 cut(s) 1285
BsuI GTATCC 1 cut(s) 1075
BsuRI GGCC 4 cut(s) 469, 856, 958, 1016
BtgI CCRYGG 2 cut(s) 58, 936
BtgZI GCGATG 1 cut(s) 184
BtsCI GGATG 5 cut(s) 271, 558, 855, 1385, 1423
BtsI GCAGTG 2 cut(s) 766, 1323
BtsIMutI CAGTG 3 cut(s) 499, 766, 1323
BveI ACCTGC 1 cut(s) 513
Cac8I GCNNGC 1 cut(s) 1472
CciI TCATGA 1 cut(s) 69
Cfr13I GGNCC 1 cut(s) 957
CseI GACGC 1 cut(s) 1121
CsiI ACCWGGT 1 cut(s) 646
Csp6I GTAC 4 cut(s) 300, 485, 843, 1270
CviAII CATG 7 cut(s) 59, 70, 289, 517, 576, 937, 1251
CviQI GTAC 4 cut(s) 300, 485, 843, 1270
DdeI CTNAG 5 cut(s) 273, 405, 726, 1243, 1492
DpnI GATC 5 cut(s) 128, 339, 399, 582, 1287
DpnII GATC 5 cut(s) 126, 337, 397, 580, 1285
DraI TTTAAA 2 cut(s) 480, 1276
EaeI YGGCCR 1 cut(s) 1014
Eam1104I CTCTTC 1 cut(s) 1066
EarI CTCTTC 1 cut(s) 1066
Eco130I CCWWGG 3 cut(s) 58, 936, 994
Eco24I GRGCYC 1 cut(s) 1384
Eco31I GGTCTC 1 cut(s) 436
Eco32I GATATC 1 cut(s) 1486
Eco57I CTGAAG 3 cut(s) 136, 611, 746
Eco91I GGTNACC 1 cut(s) 638
EcoNI CCTNNNNNAGG 1 cut(s) 253
EcoO65I GGTNACC 1 cut(s) 638
EcoRII CCWGG 1 cut(s) 646
EcoRV GATATC 1 cut(s) 1486
EcoT14I CCWWGG 3 cut(s) 58, 936, 994
EcoT22I ATGCAT 1 cut(s) 1222
EcoT38I GRGCYC 1 cut(s) 1384
ErhI CCWWGG 3 cut(s) 58, 936, 994
FaeI CATG 7 cut(s) 62, 73, 292, 520, 579, 940, 1254
FaqI GGGAC 1 cut(s) 1063
FatI CATG 7 cut(s) 58, 69, 288, 516, 575, 936, 1250
FauI CCCGC 1 cut(s) 602
Fnu4HI GCNGC 8 cut(s) 27, 187, 190, 204, 270, 333, 956, 1163
FokI GGATG 5 cut(s) 278, 565, 862, 1372, 1430
FriOI GRGCYC 1 cut(s) 1384
Fsp4HI GCNGC 8 cut(s) 27, 187, 190, 204, 270, 333, 956, 1163
FspBI CTAG 2 cut(s) 1088, 1157
GluI GCNGC 8 cut(s) 27, 187, 190, 204, 270, 333, 956, 1163
GsuI CTGGAG 1 cut(s) 274
HaeIII GGCC 4 cut(s) 469, 856, 958, 1016
HapII CCGG 2 cut(s) 183, 1068
HgaI GACGC 1 cut(s) 1121
Hin1II CATG 7 cut(s) 62, 73, 292, 520, 579, 940, 1254
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 6 cut(s) 19, 234, 808, 988, 1433, 1448
HpaII CCGG 2 cut(s) 183, 1068
HphI GGTGA 4 cut(s) 238, 440, 997, 1493
Hpy166II GTNNAC 3 cut(s) 371, 723, 1270
Hpy188I TCNGA 4 cut(s) 155, 286, 1417, 1438
Hpy188III TCNNGA 8 cut(s) 70, 238, 253, 395, 527, 632, 1068, 1097
Hpy8I GTNNAC 3 cut(s) 371, 723, 1270
HpyAV CCTTC 8 cut(s) 160, 185, 188, 259, 407, 456, 586, 739
HpyCH4III ACNGT 5 cut(s) 96, 489, 886, 907, 1404
HpyCH4IV ACGT 2 cut(s) 220, 699
HpyCH4V TGCA 8 cut(s) 452, 508, 757, 771, 1220, 1250, 1346, 1352
HpyF10VI GCNNNNNNNGC 7 cut(s) 82, 195, 763, 785, 1162, 1238, 1247
HpyF3I CTNAG 5 cut(s) 273, 405, 726, 1243, 1492
HpySE526I ACGT 2 cut(s) 220, 699
Hsp92II CATG 7 cut(s) 62, 73, 292, 520, 579, 940, 1254
Kpn2I TCCGGA 1 cut(s) 1067
Kzo9I GATC 5 cut(s) 126, 337, 397, 580, 1285
LguI GCTCTTC 1 cut(s) 1066
LmnI GCTCC 4 cut(s) 258, 664, 890, 1387
Lsp1109I GCAGC 4 cut(s) 201, 256, 344, 1174
LweI GCATC 4 cut(s) 207, 256, 780, 1090
MabI ACCWGGT 1 cut(s) 646
MaeI CTAG 2 cut(s) 1088, 1157
MaeII ACGT 2 cut(s) 220, 699
MaeIII GTNAC 5 cut(s) 598, 638, 687, 865, 880
MalI GATC 5 cut(s) 128, 339, 399, 582, 1287
MbiI CCGCTC 2 cut(s) 26, 609
MboI GATC 5 cut(s) 126, 337, 397, 580, 1285
MfeI CAATTG 1 cut(s) 858
MflI RGATCY 1 cut(s) 1285
MhlI GDGCHC 1 cut(s) 1384
MlsI TGGCCA 1 cut(s) 1016
MluCI AATT 3 cut(s) 714, 832, 858
MluNI TGGCCA 1 cut(s) 1016
MmeI TCCRAC 1 cut(s) 868
Mox20I TGGCCA 1 cut(s) 1016
Mph1103I ATGCAT 1 cut(s) 1222
MroI TCCGGA 1 cut(s) 1067
MscI TGGCCA 1 cut(s) 1016
MseI TTAA 5 cut(s) 479, 536, 837, 1029, 1275
MslI CAYNNNNRTG 3 cut(s) 68, 515, 1357
Msp20I TGGCCA 1 cut(s) 1016
MspA1I CMGCKG 2 cut(s) 335, 779
MspI CCGG 2 cut(s) 183, 1068
MspR9I CCNGG 2 cut(s) 183, 648
MunI CAATTG 1 cut(s) 858
Mva1269I GAATGC 1 cut(s) 51
MvaI CCWGG 1 cut(s) 648
MwoI GCNNNNNNNGC 7 cut(s) 82, 195, 763, 785, 1162, 1238, 1247
NciI CCSGG 1 cut(s) 183
NcoI CCATGG 2 cut(s) 58, 936
NdeII GATC 5 cut(s) 126, 337, 397, 580, 1285
NlaIII CATG 7 cut(s) 62, 73, 292, 520, 579, 940, 1254
NlaIV GGNNCC 2 cut(s) 660, 1383
NmuCI GTSAC 2 cut(s) 687, 880
NsiI ATGCAT 1 cut(s) 1222
NspI RCATGY 1 cut(s) 579
PagI TCATGA 1 cut(s) 69
PciSI GCTCTTC 1 cut(s) 1066
PctI GAATGC 1 cut(s) 51
PfeI GAWTC 6 cut(s) 19, 234, 808, 988, 1433, 1448
PflMI CCANNNNNTGG 1 cut(s) 548
PkrI GCNGC 8 cut(s) 28, 188, 191, 205, 271, 334, 957, 1164
Ppu21I YACGTR 1 cut(s) 700
PsiI TTATAA 1 cut(s) 320
Psp6I CCWGG 1 cut(s) 646
PspEI GGTNACC 1 cut(s) 638
PspGI CCWGG 1 cut(s) 646
PspN4I GGNNCC 2 cut(s) 660, 1383
PspPI GGNCC 1 cut(s) 957
PsrI GAACNNNNNNTAC 4 cut(s) 548, 580, 1193, 1225
PsuI RGATCY 1 cut(s) 1285
PvuII CAGCTG 2 cut(s) 335, 779
RsaI GTAC 4 cut(s) 301, 486, 844, 1271
RsaNI GTAC 4 cut(s) 300, 485, 843, 1270
RseI CAYNNNNRTG 3 cut(s) 68, 515, 1357
SapI GCTCTTC 1 cut(s) 1066
SaqAI TTAA 5 cut(s) 479, 536, 837, 1029, 1275
SatI GCNGC 8 cut(s) 27, 187, 190, 204, 270, 333, 956, 1163
Sau3AI GATC 5 cut(s) 126, 337, 397, 580, 1285
Sau96I GGNCC 1 cut(s) 957
ScaI AGTACT 1 cut(s) 301
ScrFI CCNGG 2 cut(s) 183, 648
SduI GDGCHC 1 cut(s) 1384
SexAI ACCWGGT 1 cut(s) 646
SfaNI GCATC 4 cut(s) 207, 256, 780, 1090
SfcI CTRYAG 1 cut(s) 1166
SmiMI CAYNNNNRTG 3 cut(s) 68, 515, 1357
Sse9I AATT 3 cut(s) 714, 832, 858
SsiI CCGC 6 cut(s) 26, 186, 204, 216, 609, 955
SspI AATATT 3 cut(s) 324, 349, 1033
SspMI CTAG 2 cut(s) 1088, 1157
StyD4I CCNGG 2 cut(s) 181, 646
StyI CCWWGG 3 cut(s) 58, 936, 994
TaaI ACNGT 5 cut(s) 96, 489, 886, 907, 1404
TaiI ACGT 2 cut(s) 223, 702
TaqI TCGA 5 cut(s) 22, 125, 232, 237, 652
TasI AATT 3 cut(s) 714, 832, 858
TatI WGTACW 3 cut(s) 299, 842, 1269
TauI GCSGC 4 cut(s) 29, 189, 206, 958
TfiI GAWTC 6 cut(s) 19, 234, 808, 988, 1433, 1448
Tru1I TTAA 5 cut(s) 479, 536, 837, 1029, 1275
Tru9I TTAA 5 cut(s) 479, 536, 837, 1029, 1275
TscAI CASTG 3 cut(s) 499, 773, 1330
TseFI GTSAC 2 cut(s) 687, 880
TseI GCWGC 4 cut(s) 189, 269, 332, 1162
Tsp45I GTSAC 2 cut(s) 687, 880
TspDTI ATGAA 7 cut(s) 58, 86, 305, 683, 1434, 1458, 1548
TspRI CASTG 3 cut(s) 499, 773, 1330
Van91I CCANNNNNTGG 1 cut(s) 548
XagI CCTNNNNNAGG 1 cut(s) 253
XceI RCATGY 1 cut(s) 579
XspI CTAG 2 cut(s) 1088, 1157
ZrmI AGTACT 1 cut(s) 301
Zsp2I ATGCAT 1 cut(s) 1222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.