pycom15g37150

Transmembrane protein 87B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0011281
Physical Location & Seq
Forward (+)
37050638 .. 37054644
4007 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g37150.1

Sequence Viewer

Length: 1569 bp
ATGGATTTCACAACCCAGATTCGAGCGGCGAGAAGAAGAAGAAGAAGCAACGGCATTCTCCATGGCATTTTCATGAAGCTCTCTCTGCTTCTCCTACTGTCATTCCTTCCAAATCCCACAAACGCCTCGATCCACATCTACGACAACCAAACCTTCAGAGAAGTTGGCAATGCTTACCTTCTACCCGGCGGCAGCGAAGGCATCGCCGCCACTCTCTCTGCCCCTTCTTACATTCGATTTGAGAAAATCACCTTCTGGAGGAGCAAGGATGCTGCTAAGAAGCTTTCAGACATGGAGCATAGTACTGGGTTGATACAAGTTATAATATTTGAGGCAGCTGATCGTAATAATATTGGTGGTTCTGCTTATGGTGGACAAAGAGATATATGTTGCTCTCCTGATCTCGCTAAGATGGAAGGTTGTAAGCAAGGTGAAGTCATTAGGAGACCTTCTGCAACAGATAATAAATGGCCTATTGTTTTAAATGTACAGTTCAGTGGGAACTTCCTGCACACAAACATGGATTATCAAGAGATTTTAATCCCAAAAACTGGGATGTATAACTTGTTTTTTGTAGCATGTGATCCAAAACTGAAGGGAGTAACTATGAGCGGGAAGACTTTGTGGAAAAATCCTGATGGTTACCTACCGGGTCGAATGGCTCCACTAATGAAGTTTTATGTGTTTATGTCACTTGCTTATGTGTTGCTTGGAATAATTTGGTTCACTCAGTATGTGAGGTTTTGGAAGGATATTCTGCAACTTCAGCACTGCATCACAGCTGTTATTGCTCTTGGGTTGTTTGAGATGATTCTTTGGTATTGTGATTATGATAATTTTAATAGTACAGGGATGAGGCCAATTGTTGTTACAACATTGGTTGTGACGGTTGGAGCTGTGAGAAAAACAGTTTCCCGTCTTCTTATCCTCATTGTTTCCATGGGTTATGGTGTGGTGCGGCCCACTCTTGGGGGTCTTACCTCAAAGGTGATTCTCCTTGGAATAACATTTTTTCTGGCCACAGAGTTGCTTAATAGTACAGAGTATGTGGGGACAATCAATGATGTATCTGGGAGAGCAAGATTATTTCTAGTTCTTCCTGATGCTTTCTTGGACGCATTTTTGATATTATGGATTTTTACCTCTCTCTCTAAAACACTAGAGCAGCTACAGGCAAAGAGAAGCTCTGTCAAACTGGATATGTATAGGAAGTTCTCAAATGCATTAGCAGTCGCAGTCATTGCCTCAGTTGCATGGATAGTGTATGAGGTGTACTTTAAAGCAACAGATCCATTCAATGAGAAGTGGCAAAGTGCTTGGGTCATCACTGCTTTCTGGGATATTCTTGCATTTGCATTGCTGTGTGTTATCTGCTATCTTTGGGCTCCATCCCAGAGTTCTCAACGGTATGCTTACTCAGATGAAGTGGGAGAAGAATCTGACGATGAAGAATCTCAAGCTCTTACGAGGGGCAAGCCAGAGGGTGATATCAGCTTAACCAAGCAAGAGAGGATTGAAAGTGCTGAAGATTTTGATGAAGATGATACAGAAGAAGACAAAAGGGAATGA

Protein Analysis

523

Amino Acids

58.94

Weight (kDa)

6.08

Isoelectric Point (pI)

43.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GOST_TM PF06814 222 - 468 5.2e-92 GOST, seven transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011281)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01070 AT2G01070
fragaria_vesca FvH4_5g30110 FvH4_5g30110
malus_domestica MD08G1225000.v1.1 MD15G1418500.v1.1
prunus_persica Prupe.1G562600_v2.0.a1
pyrus_communis pycom08g19610 pycom08g19620 pycom15g37150
rosa_chinensis RchiOBHm_Chr7g0224311 RchiOBHm_Chr7g0224371
rosa_laevigata RLG00000001905
rosa_multiflora Rmu_sc0025639.1_g000001
rosa_roxburghii Rroxscaffold_3G00234870
rosa_rugosa Rorug07G0222600
rosa_samantha Rh7AG380800 Rh7BG358500 Rh7DG367600
rosa_wichuraiana Rw7G031110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 323
AccB7I CCANNNNNTGG 1 cut(s) 551
AccBSI CCGCTC 2 cut(s) 26, 612
AciI CCGC 5 cut(s) 26, 189, 207, 612, 958
AclWI GGATC 3 cut(s) 124, 578, 1283
AcoI YGGCCR 1 cut(s) 1017
AcuI CTGAAG 4 cut(s) 139, 614, 749, 1545
AfaI GTAC 5 cut(s) 304, 489, 847, 1039, 1274
AfiI CCNNNNNNNGG 4 cut(s) 258, 551, 968, 969
AgsI TTSAA 2 cut(s) 1297, 1517
AluBI AGCT 9 cut(s) 79, 283, 338, 782, 896, 1168, 1185, 1460, 1494
AluI AGCT 9 cut(s) 79, 283, 338, 782, 896, 1168, 1185, 1460, 1494
Alw26I GTCTC 1 cut(s) 439
AlwI GGATC 3 cut(s) 124, 578, 1283
AoxI GGCC 4 cut(s) 470, 857, 959, 1017
ApeKI GCWGC 4 cut(s) 192, 272, 335, 1165
AspS9I GGNCC 1 cut(s) 960
AsuC2I CCSGG 2 cut(s) 186, 651
AsuHPI GGTGA 4 cut(s) 241, 443, 1000, 1496
BalI TGGCCA 1 cut(s) 1019
BanII GRGCYC 1 cut(s) 1387
BbsI GAAGAC 3 cut(s) 623, 911, 1560
BbvI GCAGC 4 cut(s) 204, 259, 347, 1177
BccI CCATC 3 cut(s) 406, 632, 1396
BceAI ACGGC 1 cut(s) 67
BcnI CCSGG 2 cut(s) 186, 651
BcoDI GTCTC 1 cut(s) 439
BfaI CTAG 2 cut(s) 1091, 1160
BfmI CTRYAG 1 cut(s) 1169
BisI GCNGC 8 cut(s) 27, 190, 193, 207, 273, 336, 959, 1166
BlsI GCNGC 8 cut(s) 28, 191, 194, 208, 274, 337, 960, 1167
BmcAI AGTACT 1 cut(s) 304
Bme1390I CCNGG 2 cut(s) 186, 651
BmgT120I GGNCC 1 cut(s) 960
BmiI GGNNCC 2 cut(s) 663, 1386
BmrFI CCNGG 2 cut(s) 186, 651
BmrI ACTGGG 2 cut(s) 315, 561
BmsI GCATC 4 cut(s) 210, 259, 783, 1093
BmuI ACTGGG 2 cut(s) 315, 561
BpiI GAAGAC 3 cut(s) 623, 911, 1560
BpmI CTGGAG 1 cut(s) 277
BpuEI CTTGAG 1 cut(s) 1440
BpuMI CCSGG 2 cut(s) 186, 651
BsaBI GATNNNNATC 2 cut(s) 134, 539
BsaI GGTCTC 1 cut(s) 439
BsaJI CCNNGG 3 cut(s) 61, 939, 997
Bsc4I CCNNNNNNNGG 4 cut(s) 258, 551, 968, 969
Bse1I ACTGG 3 cut(s) 310, 556, 1200
Bse3DI GCAATG 3 cut(s) 175, 1239, 1355
Bse8I GATNNNNATC 2 cut(s) 134, 539
BseDI CCNNGG 3 cut(s) 61, 939, 997
BseGI GGATG 4 cut(s) 274, 561, 858, 1388
BseJI GATNNNNATC 2 cut(s) 134, 539
BseLI CCNNNNNNNGG 4 cut(s) 258, 551, 968, 969
BseMI GCAATG 3 cut(s) 175, 1239, 1355
BseMII CTCAG 3 cut(s) 743, 1260, 1431
BseNI ACTGG 3 cut(s) 310, 556, 1200
BseRI GAGGAG 1 cut(s) 274
BseXI GCAGC 4 cut(s) 204, 259, 347, 1177
BsgI GTGCAG 1 cut(s) 494
BshFI GGCC 4 cut(s) 472, 859, 961, 1019
BsiSI CCGG 2 cut(s) 186, 650
BslFI GGGAC 1 cut(s) 1066
BslI CCNNNNNNNGG 4 cut(s) 258, 551, 968, 969
BsmAI GTCTC 1 cut(s) 439
BsmFI GGGAC 1 cut(s) 1066
BsmI GAATGC 1 cut(s) 54
BsnI GGCC 4 cut(s) 472, 859, 961, 1019
Bso31I GGTCTC 1 cut(s) 439
Bsp1286I GDGCHC 1 cut(s) 1387
Bsp1407I TGTACA 1 cut(s) 487
Bsp143I GATC 5 cut(s) 129, 340, 400, 583, 1288
Bsp19I CCATGG 2 cut(s) 61, 939
BspACI CCGC 5 cut(s) 26, 189, 207, 612, 958
BspANI GGCC 4 cut(s) 472, 859, 961, 1019
BspCNI CTCAG 3 cut(s) 742, 1259, 1430
BspHI TCATGA 1 cut(s) 72
BspLI GGNNCC 2 cut(s) 663, 1386
BspPI GGATC 3 cut(s) 124, 578, 1283
BspTNI GGTCTC 1 cut(s) 439
BsrBI CCGCTC 2 cut(s) 26, 612
BsrDI GCAATG 3 cut(s) 175, 1239, 1355
BsrGI TGTACA 1 cut(s) 487
BsrI ACTGG 3 cut(s) 310, 556, 1200
BssECI CCNNGG 3 cut(s) 61, 939, 997
BssMI GATC 5 cut(s) 129, 340, 400, 583, 1288
BssT1I CCWWGG 3 cut(s) 61, 939, 997
Bst4CI ACNGT 5 cut(s) 99, 492, 889, 910, 1407
BstAPI GCANNNNNTGC 1 cut(s) 1241
BstAUI TGTACA 1 cut(s) 487
BstC8I GCNNGC 1 cut(s) 1475
BstDEI CTNAG 5 cut(s) 276, 408, 729, 1246, 1417
BstDSI CCRYGG 2 cut(s) 61, 939
BstEII GGTNACC 1 cut(s) 641
BstENI CCTNNNNNAGG 1 cut(s) 256
BstF5I GGATG 4 cut(s) 274, 561, 858, 1388
BstKTI GATC 5 cut(s) 132, 343, 403, 586, 1291
BstMAI GTCTC 1 cut(s) 439
BstMBI GATC 5 cut(s) 129, 340, 400, 583, 1288
BstMWI GCNNNNNNNGC 6 cut(s) 85, 198, 766, 788, 1241, 1250
BstNSI RCATGY 1 cut(s) 582
BstPI GGTNACC 1 cut(s) 641
BstSCI CCNGG 2 cut(s) 184, 649
BstSFI CTRYAG 1 cut(s) 1169
BstV1I GCAGC 4 cut(s) 204, 259, 347, 1177
BstV2I GAAGAC 3 cut(s) 623, 911, 1560
BstX2I RGATCY 1 cut(s) 1288
BstYI RGATCY 1 cut(s) 1288
BsuRI GGCC 4 cut(s) 472, 859, 961, 1019
BtgI CCRYGG 2 cut(s) 61, 939
BtgZI GCGATG 1 cut(s) 187
BtsCI GGATG 4 cut(s) 274, 561, 858, 1388
BtsI GCAGTG 2 cut(s) 769, 1326
BtsIMutI CAGTG 3 cut(s) 502, 769, 1326
Cac8I GCNNGC 1 cut(s) 1475
CciI TCATGA 1 cut(s) 72
Cfr13I GGNCC 1 cut(s) 960
CseI GACGC 1 cut(s) 1124
Csp6I GTAC 5 cut(s) 303, 488, 846, 1038, 1273
CviAII CATG 7 cut(s) 62, 73, 292, 520, 579, 940, 1254
CviQI GTAC 5 cut(s) 303, 488, 846, 1038, 1273
DdeI CTNAG 5 cut(s) 276, 408, 729, 1246, 1417
DpnI GATC 5 cut(s) 131, 342, 402, 585, 1290
DpnII GATC 5 cut(s) 129, 340, 400, 583, 1288
DraI TTTAAA 2 cut(s) 483, 1279
EaeI YGGCCR 1 cut(s) 1017
Eco130I CCWWGG 3 cut(s) 61, 939, 997
Eco24I GRGCYC 1 cut(s) 1387
Eco31I GGTCTC 1 cut(s) 439
Eco32I GATATC 1 cut(s) 1489
Eco57I CTGAAG 4 cut(s) 139, 614, 749, 1545
Eco91I GGTNACC 1 cut(s) 641
EcoNI CCTNNNNNAGG 1 cut(s) 256
EcoO65I GGTNACC 1 cut(s) 641
EcoRV GATATC 1 cut(s) 1489
EcoT14I CCWWGG 3 cut(s) 61, 939, 997
EcoT22I ATGCAT 1 cut(s) 1225
EcoT38I GRGCYC 1 cut(s) 1387
ErhI CCWWGG 3 cut(s) 61, 939, 997
FaeI CATG 7 cut(s) 65, 76, 295, 523, 582, 943, 1257
FaqI GGGAC 1 cut(s) 1066
FatI CATG 7 cut(s) 61, 72, 291, 519, 578, 939, 1253
FauI CCCGC 1 cut(s) 605
Fnu4HI GCNGC 8 cut(s) 27, 190, 193, 207, 273, 336, 959, 1166
FokI GGATG 4 cut(s) 281, 568, 865, 1375
FriOI GRGCYC 1 cut(s) 1387
Fsp4HI GCNGC 8 cut(s) 27, 190, 193, 207, 273, 336, 959, 1166
FspBI CTAG 2 cut(s) 1091, 1160
GluI GCNGC 8 cut(s) 27, 190, 193, 207, 273, 336, 959, 1166
GsuI CTGGAG 1 cut(s) 277
HaeIII GGCC 4 cut(s) 472, 859, 961, 1019
HapII CCGG 2 cut(s) 186, 650
HgaI GACGC 1 cut(s) 1124
Hin1II CATG 7 cut(s) 65, 76, 295, 523, 582, 943, 1257
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 5 cut(s) 19, 811, 991, 1436, 1451
HpaII CCGG 2 cut(s) 186, 650
HphI GGTGA 4 cut(s) 241, 443, 1000, 1496
Hpy166II GTNNAC 3 cut(s) 374, 726, 1273
Hpy188I TCNGA 4 cut(s) 158, 289, 1420, 1441
Hpy188III TCNNGA 6 cut(s) 73, 256, 398, 530, 635, 1100
Hpy8I GTNNAC 3 cut(s) 374, 726, 1273
HpyCH4III ACNGT 5 cut(s) 99, 492, 889, 910, 1407
HpyCH4V TGCA 8 cut(s) 455, 511, 760, 774, 1223, 1253, 1349, 1355
HpyF10VI GCNNNNNNNGC 6 cut(s) 85, 198, 766, 788, 1241, 1250
HpyF3I CTNAG 5 cut(s) 276, 408, 729, 1246, 1417
Hsp92II CATG 7 cut(s) 65, 76, 295, 523, 582, 943, 1257
Kzo9I GATC 5 cut(s) 129, 340, 400, 583, 1288
LmnI GCTCC 5 cut(s) 261, 295, 667, 893, 1390
Lsp1109I GCAGC 4 cut(s) 204, 259, 347, 1177
LweI GCATC 4 cut(s) 210, 259, 783, 1093
MaeI CTAG 2 cut(s) 1091, 1160
MaeIII GTNAC 5 cut(s) 601, 641, 690, 868, 883
MalI GATC 5 cut(s) 131, 342, 402, 585, 1290
MbiI CCGCTC 2 cut(s) 26, 612
MboI GATC 5 cut(s) 129, 340, 400, 583, 1288
MfeI CAATTG 1 cut(s) 861
MflI RGATCY 1 cut(s) 1288
MhlI GDGCHC 1 cut(s) 1387
MlsI TGGCCA 1 cut(s) 1019
MluCI AATT 3 cut(s) 717, 835, 861
MluNI TGGCCA 1 cut(s) 1019
MmeI TCCRAC 1 cut(s) 871
Mox20I TGGCCA 1 cut(s) 1019
Mph1103I ATGCAT 1 cut(s) 1225
MscI TGGCCA 1 cut(s) 1019
MseI TTAA 6 cut(s) 482, 539, 840, 1032, 1278, 1496
MslI CAYNNNNRTG 3 cut(s) 71, 518, 1360
Msp20I TGGCCA 1 cut(s) 1019
MspA1I CMGCKG 2 cut(s) 338, 782
MspI CCGG 2 cut(s) 186, 650
MspR9I CCNGG 2 cut(s) 186, 651
MunI CAATTG 1 cut(s) 861
Mva1269I GAATGC 1 cut(s) 54
MwoI GCNNNNNNNGC 6 cut(s) 85, 198, 766, 788, 1241, 1250
NciI CCSGG 2 cut(s) 186, 651
NcoI CCATGG 2 cut(s) 61, 939
NdeII GATC 5 cut(s) 129, 340, 400, 583, 1288
NlaIII CATG 7 cut(s) 65, 76, 295, 523, 582, 943, 1257
NlaIV GGNNCC 2 cut(s) 663, 1386
NmuCI GTSAC 2 cut(s) 690, 883
NsiI ATGCAT 1 cut(s) 1225
NspI RCATGY 1 cut(s) 582
PagI TCATGA 1 cut(s) 72
PctI GAATGC 1 cut(s) 54
PfeI GAWTC 5 cut(s) 19, 811, 991, 1436, 1451
PflMI CCANNNNNTGG 1 cut(s) 551
PkrI GCNGC 8 cut(s) 28, 191, 194, 208, 274, 337, 960, 1167
PsiI TTATAA 1 cut(s) 323
PspEI GGTNACC 1 cut(s) 641
PspN4I GGNNCC 2 cut(s) 663, 1386
PspPI GGNCC 1 cut(s) 960
PsrI GAACNNNNNNTAC 2 cut(s) 1196, 1228
PsuI RGATCY 1 cut(s) 1288
PvuII CAGCTG 2 cut(s) 338, 782
RsaI GTAC 5 cut(s) 304, 489, 847, 1039, 1274
RsaNI GTAC 5 cut(s) 303, 488, 846, 1038, 1273
RseI CAYNNNNRTG 3 cut(s) 71, 518, 1360
SaqAI TTAA 6 cut(s) 482, 539, 840, 1032, 1278, 1496
SatI GCNGC 8 cut(s) 27, 190, 193, 207, 273, 336, 959, 1166
Sau3AI GATC 5 cut(s) 129, 340, 400, 583, 1288
Sau96I GGNCC 1 cut(s) 960
ScaI AGTACT 1 cut(s) 304
ScrFI CCNGG 2 cut(s) 186, 651
SduI GDGCHC 1 cut(s) 1387
SfaNI GCATC 4 cut(s) 210, 259, 783, 1093
SfcI CTRYAG 1 cut(s) 1169
SmiMI CAYNNNNRTG 3 cut(s) 71, 518, 1360
SmlI CTYRAG 1 cut(s) 1455
SmoI CTYRAG 1 cut(s) 1455
Sse9I AATT 3 cut(s) 717, 835, 861
SsiI CCGC 5 cut(s) 26, 189, 207, 612, 958
SspI AATATT 2 cut(s) 327, 352
SspMI CTAG 2 cut(s) 1091, 1160
StyD4I CCNGG 2 cut(s) 184, 649
StyI CCWWGG 3 cut(s) 61, 939, 997
TaaI ACNGT 5 cut(s) 99, 492, 889, 910, 1407
TaqI TCGA 4 cut(s) 22, 128, 235, 655
TasI AATT 3 cut(s) 717, 835, 861
TatI WGTACW 5 cut(s) 302, 487, 845, 1037, 1272
TauI GCSGC 4 cut(s) 29, 192, 209, 961
TfiI GAWTC 5 cut(s) 19, 811, 991, 1436, 1451
Tru1I TTAA 6 cut(s) 482, 539, 840, 1032, 1278, 1496
Tru9I TTAA 6 cut(s) 482, 539, 840, 1032, 1278, 1496
TscAI CASTG 3 cut(s) 502, 776, 1333
TseFI GTSAC 2 cut(s) 690, 883
TseI GCWGC 4 cut(s) 192, 272, 335, 1165
Tsp45I GTSAC 2 cut(s) 690, 883
TspDTI ATGAA 6 cut(s) 61, 89, 686, 1437, 1461, 1551
TspRI CASTG 3 cut(s) 502, 776, 1333
Van91I CCANNNNNTGG 1 cut(s) 551
XagI CCTNNNNNAGG 1 cut(s) 256
XceI RCATGY 1 cut(s) 582
XspI CTAG 2 cut(s) 1091, 1160
ZrmI AGTACT 1 cut(s) 304
Zsp2I ATGCAT 1 cut(s) 1225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.