Rw7G031110

Transmembrane protein 87B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0011281
Physical Location & Seq
Forward (+)
44314529 .. 44318294
3766 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G031110.1

Sequence Viewer

Length: 1557 bp
ATGGATTTCACATTCCAGAGAAGAGGAAGAGGAAGAAGGAGAAGACTCACCGACCTCTCGCTGCTCCTCATCCTCCTCCTCCTTTTAACAACATTATTCAATTCCACTCCCGCCAACGCCTCCATCCATATCTACAGCGATGAAATCTTCCGGGAAGTCGGCAATGCTTTCCTCCTCTCCGGCGGCAGCGAAGGCATCTCCGCCTCTCCCTCCTCCCGCTCTTTTATCCGATTCGAGAATATCACCTTCTGGAGGAGCGAGGCTGCTGTTGACAAGAAGCATAGTAATGGGTTGATACAGGTTATCGTTTTTGAAGCCGCGGATCGGAATAATATCGGTGGTTCTGCTTATGGGGGACAGAGATCTATATGTTGCACCCCTGATCTTGCTAAGATGGAAGGTTGCAAGCAAGGTGAAGTCATTAGGAGACCTTCTGCCACAGATGTTAATTGGCCCATTGTTATAAATGTACACTTCAGAAGGAACAGTCTCTCTAAAAGTATGGATTATAAAGAGATTTCGATCACCAAAACGGGGATGTATAACTTGTTTTTTGTAGCCTGTGATCCAAAGCTGAAGGAACTAGTAATGAGCGGGAAGACGGTATGGAAAAGTCCCGATGGTTATCTACCTGGTCGAATGGCTCCGCTGATGAAATTTTACGTGTATATGGCACTTGCTTATGTGCTGATTGGTGTAATTTGGCTCACTCAGTATGTGAGGTTCTGGAAGGATATTCTGCAACTTCAGCATTGCATCACGGCTGTCATTGCTCTTGGATTGTTTGAGATGATTCTTTGGTATTCCGACTATGTCAATTTTAATAATACAGGGATGAGGCCAGTTTTTCTTACGACATTAGTTGTAACAGTTGGAGCTGTGAGAAAAACAGTTTCCCGTCTCCTTATCCTCACTGTTTCTATGGGCTATGGTGTAGTACGGCCTACTCTTGGGGGTCTTACCTCCAAGGTGCTTCTGCTTGGAATAACATTTTTTCTGGCAACAGAGTTGCTTAACATTACCGAGTTTGTTGGGACAATTAATGATGTATCAGGAAGAGCGAGATTATTTCTAGTTCTTCCTGATGCTTTCTTGGACGCATTTTTAATCCTGTGGATTTTCACCTCTCTCTCCAAAACACTAGAACAGCTACAGACAAAGAGAAGCTCTGTCAAGTTGGATATGTATAGGAAGTTCTCAAATGCATTAGCAGTGACAGTCATTGCCTCAGTTGCATGGATAGCTTATGAGGTGTACTTCAAAGCAACAGATCCCTTCAATGAGAAGTGGCATAGTGCTTGGATCATCACTGCTTTCTGGGATATTCTTGCATTTGCATTGCTGGGCGTTATCTGCTATCTTTGGGCACCATCTCAGAACTCTCAGAGGTATGCATACTCAGAGAAGCTAGGAGAAGAATCTGATGATGAAGAATCTCAGACTCTAACTGGGGGCAAGCCAGAGGGCGATATAAGCTTAGTCAATCAAGAAAAGAAGAGTGGTGGAAGCACCGGAGATTCTGATGAAGAGGACACAGAAGAAGATAAGAGGGAATGA

Protein Analysis

518

Amino Acids

58.27

Weight (kDa)

8.06

Isoelectric Point (pI)

47.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GOST_TM PF06814 216 - 462 2.9e-89 GOST, seven transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011281)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01070 AT2G01070
fragaria_vesca FvH4_5g30110 FvH4_5g30110
malus_domestica MD08G1225000.v1.1 MD15G1418500.v1.1
prunus_persica Prupe.1G562600_v2.0.a1
pyrus_communis pycom08g19610 pycom08g19620 pycom15g37150
rosa_chinensis RchiOBHm_Chr7g0224311 RchiOBHm_Chr7g0224371
rosa_laevigata RLG00000001905
rosa_multiflora Rmu_sc0025639.1_g000001
rosa_roxburghii Rroxscaffold_3G00234870
rosa_rugosa Rorug07G0222600
rosa_samantha Rh7AG380800 Rh7BG358500 Rh7DG367600
rosa_wichuraiana Rw7G031110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 464, 510
AccB1I GGYRCC 1 cut(s) 1366
AccBSI CCGCTC 2 cut(s) 219, 594
AccII CGCG 1 cut(s) 320
AciI CCGC 8 cut(s) 111, 183, 201, 217, 318, 320, 594, 647
AclWI GGATC 4 cut(s) 330, 560, 1265, 1310
AcsI RAATTY 1 cut(s) 656
AcuI CTGAAG 3 cut(s) 460, 596, 731
AfaI GTAC 3 cut(s) 471, 939, 1256
AfiI CCNNNNNNNGG 4 cut(s) 252, 324, 534, 950
AflIII ACRYGT 1 cut(s) 663
AgsI TTSAA 4 cut(s) 100, 314, 1261, 1279
AhlI ACTAGT 1 cut(s) 583
AjnI CCWGG 1 cut(s) 631
AluBI AGCT 7 cut(s) 574, 878, 1150, 1167, 1244, 1408, 1476
AluI AGCT 7 cut(s) 574, 878, 1150, 1167, 1244, 1408, 1476
Alw26I GTCTC 3 cut(s) 421, 494, 905
AlwI GGATC 4 cut(s) 330, 560, 1265, 1310
AoxI GGCC 3 cut(s) 452, 839, 941
ApeKI GCWGC 3 cut(s) 61, 186, 263
ApoI RAATTY 1 cut(s) 656
AseI ATTAAT 1 cut(s) 1041
AspS9I GGNCC 1 cut(s) 453
AsuC2I CCSGG 1 cut(s) 152
AsuHPI GGTGA 5 cut(s) 40, 235, 425, 517, 1114
BaeGI GKGCMC 1 cut(s) 1369
BanI GGYRCC 1 cut(s) 1366
BbsI GAAGAC 2 cut(s) 49, 605
BbvI GCAGC 3 cut(s) 48, 198, 250
BccI CCATC 4 cut(s) 131, 388, 614, 1378
BceAI ACGGC 2 cut(s) 777, 956
BciT130I CCWGG 1 cut(s) 633
BcnI CCSGG 1 cut(s) 152
BcoDI GTCTC 3 cut(s) 421, 494, 905
BcuI ACTAGT 1 cut(s) 583
BfaI CTAG 4 cut(s) 584, 1073, 1142, 1409
BfmI CTRYAG 2 cut(s) 133, 1151
BglII AGATCT 1 cut(s) 362
BisI GCNGC 5 cut(s) 62, 184, 187, 264, 318
BlsI GCNGC 5 cut(s) 63, 185, 188, 265, 319
Bme1390I CCNGG 2 cut(s) 152, 633
BmgT120I GGNCC 1 cut(s) 453
BmiI GGNNCC 2 cut(s) 645, 1368
BmrFI CCNGG 2 cut(s) 152, 633
BmrI ACTGGG 1 cut(s) 1458
BmsI GCATC 3 cut(s) 204, 765, 1075
BmuI ACTGGG 1 cut(s) 1458
BpiI GAAGAC 2 cut(s) 49, 605
BpmI CTGGAG 1 cut(s) 271
BpuMI CCSGG 1 cut(s) 152
BsaAI YACGTR 1 cut(s) 664
BsaBI GATNNNNATC 2 cut(s) 521, 624
BsaI GGTCTC 1 cut(s) 421
BsaJI CCNNGG 2 cut(s) 318, 966
BsaWI WCCGGW 1 cut(s) 1511
Bsc4I CCNNNNNNNGG 4 cut(s) 252, 324, 534, 950
Bse1I ACTGG 2 cut(s) 842, 1453
Bse3DI GCAATG 5 cut(s) 169, 751, 768, 1221, 1337
Bse8I GATNNNNATC 2 cut(s) 521, 624
BseBI CCWGG 1 cut(s) 633
BseDI CCNNGG 2 cut(s) 318, 966
BseGI GGATG 4 cut(s) 69, 123, 543, 840
BseJI GATNNNNATC 2 cut(s) 521, 624
BseLI CCNNNNNNNGG 4 cut(s) 252, 324, 534, 950
BseMI GCAATG 5 cut(s) 169, 751, 768, 1221, 1337
BseMII CTCAG 6 cut(s) 725, 1242, 1388, 1397, 1413, 1451
BseNI ACTGG 2 cut(s) 842, 1453
BseRI GAGGAG 6 cut(s) 56, 65, 68, 164, 202, 268
BseSI GKGCMC 1 cut(s) 1369
BseXI GCAGC 3 cut(s) 48, 198, 250
BseYI CCCAGC 1 cut(s) 1342
Bsh1236I CGCG 1 cut(s) 320
BshFI GGCC 3 cut(s) 454, 841, 943
BshNI GGYRCC 1 cut(s) 1366
BsiSI CCGG 3 cut(s) 151, 180, 1512
BslFI GGGAC 3 cut(s) 369, 600, 1048
BslI CCNNNNNNNGG 4 cut(s) 252, 324, 534, 950
BsmAI GTCTC 3 cut(s) 421, 494, 905
BsmBI CGTCTC 1 cut(s) 905
BsmFI GGGAC 3 cut(s) 369, 600, 1048
BsnI GGCC 3 cut(s) 454, 841, 943
Bso31I GGTCTC 1 cut(s) 421
Bsp1286I GDGCHC 1 cut(s) 1369
Bsp1407I TGTACA 1 cut(s) 469
Bsp143I GATC 7 cut(s) 322, 362, 382, 522, 565, 1270, 1302
BspACI CCGC 8 cut(s) 111, 183, 201, 217, 318, 320, 594, 647
BspANI GGCC 3 cut(s) 454, 841, 943
BspCNI CTCAG 6 cut(s) 724, 1241, 1387, 1396, 1412, 1450
BspFNI CGCG 1 cut(s) 320
BspLI GGNNCC 2 cut(s) 645, 1368
BspPI GGATC 4 cut(s) 330, 560, 1265, 1310
BspQI GCTCTTC 1 cut(s) 1051
BspT107I GGYRCC 1 cut(s) 1366
BspTNI GGTCTC 1 cut(s) 421
BsrBI CCGCTC 2 cut(s) 219, 594
BsrDI GCAATG 5 cut(s) 169, 751, 768, 1221, 1337
BsrGI TGTACA 1 cut(s) 469
BsrI ACTGG 2 cut(s) 842, 1453
BssECI CCNNGG 2 cut(s) 318, 966
BssMI GATC 7 cut(s) 322, 362, 382, 522, 565, 1270, 1302
BssT1I CCWWGG 1 cut(s) 966
Bst2UI CCWGG 1 cut(s) 633
Bst4CI ACNGT 6 cut(s) 488, 604, 871, 892, 916, 1219
Bst6I CTCTTC 5 cut(s) 16, 22, 1051, 1490, 1521
BstAUI TGTACA 1 cut(s) 469
BstBAI YACGTR 1 cut(s) 664
BstC8I GCNNGC 2 cut(s) 407, 1457
BstDEI CTNAG 8 cut(s) 390, 711, 1228, 1374, 1383, 1399, 1437, 1477
BstDSI CCRYGG 1 cut(s) 318
BstENI CCTNNNNNAGG 1 cut(s) 250
BstF5I GGATG 4 cut(s) 69, 123, 543, 840
BstFNI CGCG 1 cut(s) 320
BstKTI GATC 7 cut(s) 325, 365, 385, 525, 568, 1273, 1305
BstMAI GTCTC 3 cut(s) 421, 494, 905
BstMBI GATC 7 cut(s) 322, 362, 382, 522, 565, 1270, 1302
BstMWI GCNNNNNNNGC 7 cut(s) 192, 748, 770, 1232, 1241, 1353, 1473
BstNI CCWGG 1 cut(s) 633
BstSCI CCNGG 2 cut(s) 150, 631
BstSFI CTRYAG 2 cut(s) 133, 1151
BstSLI GKGCMC 1 cut(s) 1369
BstUI CGCG 1 cut(s) 320
BstV1I GCAGC 3 cut(s) 48, 198, 250
BstV2I GAAGAC 2 cut(s) 49, 605
BstX2I RGATCY 2 cut(s) 362, 1270
BstYI RGATCY 2 cut(s) 362, 1270
BsuRI GGCC 3 cut(s) 454, 841, 943
BtgI CCRYGG 1 cut(s) 318
BtgZI GCGATG 1 cut(s) 153
BtsCI GGATG 4 cut(s) 69, 123, 543, 840
BtsI GCAGTG 2 cut(s) 1218, 1308
BtsIMutI CAGTG 3 cut(s) 912, 1218, 1308
Cac8I GCNNGC 2 cut(s) 407, 1457
Cfr13I GGNCC 1 cut(s) 453
Cfr42I CCGCGG 1 cut(s) 321
CseI GACGC 1 cut(s) 1106
CsiI ACCWGGT 1 cut(s) 631
Csp6I GTAC 3 cut(s) 470, 938, 1255
CviAII CATG 1 cut(s) 1236
CviQI GTAC 3 cut(s) 470, 938, 1255
DdeI CTNAG 8 cut(s) 390, 711, 1228, 1374, 1383, 1399, 1437, 1477
DpnI GATC 7 cut(s) 324, 364, 384, 524, 567, 1272, 1304
DpnII GATC 7 cut(s) 322, 362, 382, 522, 565, 1270, 1302
Eam1104I CTCTTC 5 cut(s) 16, 22, 1051, 1490, 1521
EarI CTCTTC 5 cut(s) 16, 22, 1051, 1490, 1521
EciI GGCGGA 1 cut(s) 190
Eco130I CCWWGG 1 cut(s) 966
Eco31I GGTCTC 1 cut(s) 421
Eco57I CTGAAG 3 cut(s) 460, 596, 731
EcoNI CCTNNNNNAGG 1 cut(s) 250
EcoRII CCWGG 1 cut(s) 631
EcoT14I CCWWGG 1 cut(s) 966
EcoT22I ATGCAT 2 cut(s) 1207, 1396
ErhI CCWWGG 1 cut(s) 966
Esp3I CGTCTC 1 cut(s) 905
FaeI CATG 1 cut(s) 1239
FaqI GGGAC 3 cut(s) 369, 600, 1048
FatI CATG 1 cut(s) 1235
FauI CCCGC 3 cut(s) 118, 224, 587
Fnu4HI GCNGC 5 cut(s) 62, 184, 187, 264, 318
FokI GGATG 4 cut(s) 56, 110, 550, 847
Fsp4HI GCNGC 5 cut(s) 62, 184, 187, 264, 318
FspBI CTAG 4 cut(s) 584, 1073, 1142, 1409
GluI GCNGC 5 cut(s) 62, 184, 187, 264, 318
GsaI CCCAGC 1 cut(s) 1346
GsuI CTGGAG 1 cut(s) 271
HaeIII GGCC 3 cut(s) 454, 841, 943
HapII CCGG 3 cut(s) 151, 180, 1512
HgaI GACGC 1 cut(s) 1106
Hin1II CATG 1 cut(s) 1239
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HindIII AAGCTT 1 cut(s) 1474
HinfI GANTC 7 cut(s) 45, 231, 793, 1418, 1433, 1441, 1517
HpaII CCGG 3 cut(s) 151, 180, 1512
HphI GGTGA 5 cut(s) 40, 235, 425, 517, 1114
Hpy166II GTNNAC 3 cut(s) 271, 472, 1255
Hpy188III TCNNGA 8 cut(s) 16, 235, 250, 617, 727, 1053, 1082, 1487
Hpy8I GTNNAC 3 cut(s) 271, 472, 1255
HpyAV CCTTC 9 cut(s) 30, 185, 256, 392, 441, 474, 571, 724, 1285
HpyCH4III ACNGT 6 cut(s) 488, 604, 871, 892, 916, 1219
HpyCH4IV ACGT 1 cut(s) 663
HpyCH4V TGCA 9 cut(s) 375, 405, 742, 756, 1205, 1235, 1331, 1337, 1394
HpyF10VI GCNNNNNNNGC 7 cut(s) 192, 748, 770, 1232, 1241, 1353, 1473
HpyF3I CTNAG 8 cut(s) 390, 711, 1228, 1374, 1383, 1399, 1437, 1477
HpySE526I ACGT 1 cut(s) 663
Hsp92II CATG 1 cut(s) 1239
KspI CCGCGG 1 cut(s) 321
Kzo9I GATC 7 cut(s) 322, 362, 382, 522, 565, 1270, 1302
LguI GCTCTTC 1 cut(s) 1051
LmnI GCTCC 4 cut(s) 69, 255, 649, 875
Lsp1109I GCAGC 3 cut(s) 48, 198, 250
LweI GCATC 3 cut(s) 204, 765, 1075
MabI ACCWGGT 1 cut(s) 631
MaeI CTAG 4 cut(s) 584, 1073, 1142, 1409
MaeII ACGT 1 cut(s) 663
MaeIII GTNAC 2 cut(s) 865, 1213
MalI GATC 7 cut(s) 324, 364, 384, 524, 567, 1272, 1304
MbiI CCGCTC 2 cut(s) 219, 594
MboI GATC 7 cut(s) 322, 362, 382, 522, 565, 1270, 1302
MflI RGATCY 2 cut(s) 362, 1270
MhlI GDGCHC 1 cut(s) 1369
MluCI AATT 6 cut(s) 100, 448, 656, 699, 817, 1038
MlyI GAGTC 2 cut(s) 39, 1435
MmeI TCCRAC 3 cut(s) 831, 853, 1158
Mph1103I ATGCAT 2 cut(s) 1207, 1396
MseI TTAA 6 cut(s) 86, 447, 822, 1014, 1041, 1106
MslI CAYNNNNRTG 1 cut(s) 285
MspA1I CMGCKG 2 cut(s) 320, 649
MspI CCGG 3 cut(s) 151, 180, 1512
MspR9I CCNGG 2 cut(s) 152, 633
MvaI CCWGG 1 cut(s) 633
MvnI CGCG 1 cut(s) 320
MwoI GCNNNNNNNGC 7 cut(s) 192, 748, 770, 1232, 1241, 1353, 1473
NciI CCSGG 1 cut(s) 152
NdeII GATC 7 cut(s) 322, 362, 382, 522, 565, 1270, 1302
NlaIII CATG 1 cut(s) 1239
NlaIV GGNNCC 2 cut(s) 645, 1368
NmuCI GTSAC 1 cut(s) 1213
NsiI ATGCAT 2 cut(s) 1207, 1396
PciSI GCTCTTC 1 cut(s) 1051
PfeI GAWTC 5 cut(s) 231, 793, 1418, 1433, 1517
PflFI GACNNNGTC 1 cut(s) 812
PfoI TCCNGGA 1 cut(s) 150
PkrI GCNGC 5 cut(s) 63, 185, 188, 265, 319
PleI GAGTC 2 cut(s) 39, 1435
PpsI GAGTC 2 cut(s) 39, 1435
Ppu21I YACGTR 1 cut(s) 664
PshBI ATTAAT 1 cut(s) 1041
PsiI TTATAA 2 cut(s) 464, 510
Psp6I CCWGG 1 cut(s) 631
PspFI CCCAGC 1 cut(s) 1342
PspGI CCWGG 1 cut(s) 631
PspN4I GGNNCC 2 cut(s) 645, 1368
PspPI GGNCC 1 cut(s) 453
PsrI GAACNNNNNNTAC 4 cut(s) 707, 739, 1178, 1210
PsuI RGATCY 2 cut(s) 362, 1270
PsyI GACNNNGTC 1 cut(s) 812
RsaI GTAC 3 cut(s) 471, 939, 1256
RsaNI GTAC 3 cut(s) 470, 938, 1255
RseI CAYNNNNRTG 1 cut(s) 285
SacII CCGCGG 1 cut(s) 321
SapI GCTCTTC 1 cut(s) 1051
SaqAI TTAA 6 cut(s) 86, 447, 822, 1014, 1041, 1106
SatI GCNGC 5 cut(s) 62, 184, 187, 264, 318
Sau3AI GATC 7 cut(s) 322, 362, 382, 522, 565, 1270, 1302
Sau96I GGNCC 1 cut(s) 453
SchI GAGTC 2 cut(s) 39, 1435
ScrFI CCNGG 2 cut(s) 152, 633
SduI GDGCHC 1 cut(s) 1369
SexAI ACCWGGT 1 cut(s) 631
SfaNI GCATC 3 cut(s) 204, 765, 1075
SfcI CTRYAG 2 cut(s) 133, 1151
Sfr303I CCGCGG 1 cut(s) 321
SgrBI CCGCGG 1 cut(s) 321
SmiMI CAYNNNNRTG 1 cut(s) 285
SpeI ACTAGT 1 cut(s) 583
Sse9I AATT 6 cut(s) 100, 448, 656, 699, 817, 1038
SsiI CCGC 8 cut(s) 111, 183, 201, 217, 318, 320, 594, 647
SspMI CTAG 4 cut(s) 584, 1073, 1142, 1409
StyD4I CCNGG 2 cut(s) 150, 631
StyI CCWWGG 1 cut(s) 966
TaaI ACNGT 6 cut(s) 488, 604, 871, 892, 916, 1219
TaiI ACGT 1 cut(s) 666
TaqI TCGA 3 cut(s) 234, 521, 637
TasI AATT 6 cut(s) 100, 448, 656, 699, 817, 1038
TatI WGTACW 2 cut(s) 469, 1254
TauI GCSGC 2 cut(s) 186, 320
TfiI GAWTC 5 cut(s) 231, 793, 1418, 1433, 1517
Tru1I TTAA 6 cut(s) 86, 447, 822, 1014, 1041, 1106
Tru9I TTAA 6 cut(s) 86, 447, 822, 1014, 1041, 1106
TscAI CASTG 3 cut(s) 919, 1218, 1315
TseFI GTSAC 1 cut(s) 1213
TseI GCWGC 3 cut(s) 61, 186, 263
Tsp45I GTSAC 1 cut(s) 1213
TspDTI ATGAA 4 cut(s) 156, 668, 1443, 1539
TspRI CASTG 3 cut(s) 919, 1218, 1315
Tth111I GACNNNGTC 1 cut(s) 812
VspI ATTAAT 1 cut(s) 1041
XagI CCTNNNNNAGG 1 cut(s) 250
XapI RAATTY 1 cut(s) 656
XspI CTAG 4 cut(s) 584, 1073, 1142, 1409
Zsp2I ATGCAT 2 cut(s) 1207, 1396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.