MD08G1243300.v1.1

RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008685
Physical Location & Seq
Reverse (-)
30868638 .. 30871555
2918 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1243300.v1.1.491

Sequence Viewer

Length: 687 bp
ATGAAAAAGCAATTGAGCAAAGCCTCTGCTCAGTTAACAGCTTCTTCTCACAAAAATAAAGAAGCTGCTGCTCCTCCTGCTACTGCAATTGTTACTGCTTCTACCGCTGCTGATTTCTTGCCATTAGAAGGCGGTCCAGCGCGGAAACTCCCCCAAGAAAAGCCTACAGAGGATGTTGCAACAGTTTTATACATTGGTCGGATACCTCATGGCTTCTATGAGAAAGAAATGGAAGGATTTTTTGGGCAATTTGGTTCGATTAAGAAATTAAGAATTGCGCGTAACAAAAAGACAGGGAAATCAAAGCATTTTGGCTTCATTGAGTTTGAAGACCCTCAGGTGGCGAAAGTTGTAGCTGACACTATGCATAACTATTTGTTGTTCGAACACGTGTTGCAAGTCCATCTTATTCCTCCGCAGAAAGTTCACCCGAAATTATGGAAAGGTTTCAACTACCGAGTCAGACCCGTGAACTGGGTTCAAATTGAGCGGAAGAGACAAGACAAGGAAAGAACAGTGGAAGAGCACAAGAAGTTAATGGGGAAGATTGTGAAGAGAGATCTGAAGCGTCAAAAGAAAATTGAGGCTGCTGGTATTGATTATAAATGCCCAGAAATTGTGGGTAGTAGTGAGGAGCCTGCTCCAAAGAAAAGGAAGACCGACCGAAAGAAGGTTGTTTCTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

229

Amino Acids

25.95

Weight (kDa)

9.98

Isoelectric Point (pI)

46.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 63 - 132 4.5e-14 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 603
AccBSI CCGCTC 1 cut(s) 490
AccII CGCG 2 cut(s) 142, 280
AciI CCGC 5 cut(s) 105, 132, 142, 416, 490
AcuI CTGAAG 1 cut(s) 584
AcvI CACGTG 1 cut(s) 391
AfiI CCNNNNNNNGG 4 cut(s) 128, 340, 474, 670
AflIII ACRYGT 2 cut(s) 388, 390
AgsI TTSAA 3 cut(s) 329, 451, 482
AjuI GAANNNNNNNTTGG 2 cut(s) 225, 257
AluBI AGCT 3 cut(s) 41, 65, 356
AluI AGCT 3 cut(s) 41, 65, 356
Alw21I GWGCWC 1 cut(s) 528
Alw26I GTCTC 1 cut(s) 490
ApeKI GCWGC 4 cut(s) 65, 68, 107, 587
Asp700I GAANNNNTTC 1 cut(s) 446
AspLEI GCGC 2 cut(s) 142, 280
AspS9I GGNCC 1 cut(s) 134
AsuHPI GGTGA 1 cut(s) 419
AsuII TTCGAA 1 cut(s) 384
AvaII GGWCC 1 cut(s) 134
AxyI CCTNAGG 1 cut(s) 336
BbrPI CACGTG 1 cut(s) 391
BbsI GAAGAC 2 cut(s) 336, 662
Bbv12I GWGCWC 1 cut(s) 528
BbvI GCAGC 4 cut(s) 52, 55, 94, 574
BccI CCATC 1 cut(s) 411
BciVI GTATCC 1 cut(s) 195
BcoDI GTCTC 1 cut(s) 490
BfmI CTRYAG 1 cut(s) 165
BfuI GTATCC 1 cut(s) 195
BglII AGATCT 1 cut(s) 559
BisI GCNGC 4 cut(s) 66, 69, 108, 588
BlsI GCNGC 4 cut(s) 67, 70, 109, 589
Bme18I GGWCC 1 cut(s) 134
BmgT120I GGNCC 1 cut(s) 134
BmiI GGNNCC 1 cut(s) 636
BmrI ACTGGG 1 cut(s) 484
BmuI ACTGGG 1 cut(s) 484
BpiI GAAGAC 2 cut(s) 336, 662
Bpu14I TTCGAA 1 cut(s) 384
BsaAI YACGTR 1 cut(s) 391
Bsc4I CCNNNNNNNGG 4 cut(s) 128, 340, 474, 670
Bse1I ACTGG 1 cut(s) 479
Bse21I CCTNAGG 1 cut(s) 336
BseGI GGATG 1 cut(s) 178
BseLI CCNNNNNNNGG 4 cut(s) 128, 340, 474, 670
BseMII CTCAG 2 cut(s) 44, 350
BseNI ACTGG 1 cut(s) 479
BseRI GAGGAG 2 cut(s) 63, 647
BseXI GCAGC 4 cut(s) 52, 55, 94, 574
Bsh1236I CGCG 2 cut(s) 142, 280
Bsh1285I CGRYCG 1 cut(s) 664
BsiEI CGRYCG 1 cut(s) 664
BsiHKAI GWGCWC 1 cut(s) 528
BslI CCNNNNNNNGG 4 cut(s) 128, 340, 474, 670
BsmAI GTCTC 1 cut(s) 490
Bsp119I TTCGAA 1 cut(s) 384
Bsp1286I GDGCHC 1 cut(s) 528
Bsp143I GATC 1 cut(s) 559
BspACI CCGC 5 cut(s) 105, 132, 142, 416, 490
BspCNI CTCAG 2 cut(s) 43, 349
BspFNI CGCG 2 cut(s) 142, 280
BspLI GGNNCC 1 cut(s) 636
BspQI GCTCTTC 1 cut(s) 516
BspT104I TTCGAA 1 cut(s) 384
BsrBI CCGCTC 1 cut(s) 490
BsrI ACTGG 1 cut(s) 479
BssMI GATC 1 cut(s) 559
Bst4CI ACNGT 2 cut(s) 184, 517
Bst6I CTCTTC 3 cut(s) 488, 516, 548
BstBAI YACGTR 1 cut(s) 391
BstBI TTCGAA 1 cut(s) 384
BstC8I GCNNGC 1 cut(s) 639
BstDEI CTNAG 2 cut(s) 30, 336
BstF5I GGATG 1 cut(s) 178
BstFNI CGCG 2 cut(s) 142, 280
BstHHI GCGC 2 cut(s) 142, 280
BstKTI GATC 1 cut(s) 562
BstMAI GTCTC 1 cut(s) 490
BstMBI GATC 1 cut(s) 559
BstMCI CGRYCG 1 cut(s) 664
BstMWI GCNNNNNNNGC 2 cut(s) 77, 104
BstSFI CTRYAG 1 cut(s) 165
BstUI CGCG 2 cut(s) 142, 280
BstV1I GCAGC 4 cut(s) 52, 55, 94, 574
BstV2I GAAGAC 2 cut(s) 336, 662
BstX2I RGATCY 1 cut(s) 559
BstYI RGATCY 1 cut(s) 559
Bsu36I CCTNAGG 1 cut(s) 336
BsuI GTATCC 1 cut(s) 195
BtsCI GGATG 1 cut(s) 178
BtsIMutI CAGTG 1 cut(s) 522
Cac8I GCNNGC 1 cut(s) 639
CfoI GCGC 2 cut(s) 142, 280
Cfr13I GGNCC 1 cut(s) 134
CseI GACGC 1 cut(s) 557
CviAII CATG 1 cut(s) 209
CviJI RGCY 9 cut(s) 23, 41, 65, 163, 213, 315, 356, 587, 637
CviKI_1 RGCY 9 cut(s) 23, 41, 65, 163, 213, 315, 356, 587, 637
DdeI CTNAG 2 cut(s) 30, 336
DpnI GATC 1 cut(s) 561
DpnII GATC 1 cut(s) 559
Eam1104I CTCTTC 3 cut(s) 488, 516, 548
EarI CTCTTC 3 cut(s) 488, 516, 548
Eco47I GGWCC 1 cut(s) 134
Eco57I CTGAAG 1 cut(s) 584
Eco72I CACGTG 1 cut(s) 391
Eco81I CCTNAGG 1 cut(s) 336
EcoT22I ATGCAT 1 cut(s) 369
FaeI CATG 1 cut(s) 212
FaiI YATR 7 cut(s) 190, 210, 219, 365, 369, 439, 603
FalI AAGNNNNNCTT 2 cut(s) 390, 422
FatI CATG 1 cut(s) 208
Fnu4HI GCNGC 4 cut(s) 66, 69, 108, 588
FokI GGATG 1 cut(s) 185
Fsp4HI GCNGC 4 cut(s) 66, 69, 108, 588
GlaI GCGC 2 cut(s) 141, 279
GluI GCNGC 4 cut(s) 66, 69, 108, 588
HgaI GACGC 1 cut(s) 557
HhaI GCGC 2 cut(s) 142, 280
Hin1II CATG 1 cut(s) 212
Hin6I GCGC 2 cut(s) 140, 278
HinP1I GCGC 2 cut(s) 140, 278
HincII GTYRAC 1 cut(s) 36
HindII GTYRAC 1 cut(s) 36
HinfI GANTC 1 cut(s) 459
HpaI GTTAAC 1 cut(s) 36
HphI GGTGA 1 cut(s) 419
Hpy166II GTNNAC 3 cut(s) 36, 427, 472
Hpy188I TCNGA 3 cut(s) 201, 464, 564
Hpy8I GTNNAC 3 cut(s) 36, 427, 472
HpyAV CCTTC 3 cut(s) 122, 227, 664
HpyCH4III ACNGT 2 cut(s) 184, 517
HpyCH4IV ACGT 1 cut(s) 390
HpyCH4V TGCA 4 cut(s) 86, 179, 367, 397
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 104
HpyF3I CTNAG 2 cut(s) 30, 336
HpySE526I ACGT 1 cut(s) 390
Hsp92II CATG 1 cut(s) 212
HspAI GCGC 2 cut(s) 140, 278
KspAI GTTAAC 1 cut(s) 36
Kzo9I GATC 1 cut(s) 559
LguI GCTCTTC 1 cut(s) 516
LmnI GCTCC 3 cut(s) 76, 634, 646
LpnPI CCDG 8 cut(s) 90, 150, 279, 323, 460, 576, 624, 651
Lsp1109I GCAGC 4 cut(s) 52, 55, 94, 574
MaeII ACGT 1 cut(s) 390
MaeIII GTNAC 2 cut(s) 91, 281
MalI GATC 1 cut(s) 561
MbiI CCGCTC 1 cut(s) 490
MboI GATC 1 cut(s) 559
MboII GAAGA 7 cut(s) 36, 341, 505, 533, 556, 565, 667
MfeI CAATTG 2 cut(s) 11, 87
MflI RGATCY 1 cut(s) 559
MhlI GDGCHC 1 cut(s) 528
MluCI AATT 9 cut(s) 11, 87, 248, 266, 273, 434, 483, 579, 615
MlyI GAGTC 1 cut(s) 468
MmeI TCCRAC 1 cut(s) 179
MnlI CCTC 8 cut(s) 34, 84, 163, 216, 345, 423, 577, 625
Mph1103I ATGCAT 1 cut(s) 369
MroXI GAANNNNTTC 1 cut(s) 446
MseI TTAA 4 cut(s) 35, 261, 269, 536
MspA1I CMGCKG 1 cut(s) 107
MunI CAATTG 2 cut(s) 11, 87
MvnI CGCG 2 cut(s) 142, 280
MwoI GCNNNNNNNGC 2 cut(s) 77, 104
NdeII GATC 1 cut(s) 559
NlaIII CATG 1 cut(s) 212
NlaIV GGNNCC 1 cut(s) 636
NsiI ATGCAT 1 cut(s) 369
NspV TTCGAA 1 cut(s) 384
PciSI GCTCTTC 1 cut(s) 516
PdmI GAANNNNTTC 1 cut(s) 446
PkrI GCNGC 4 cut(s) 67, 70, 109, 589
PleI GAGTC 1 cut(s) 467
PmaCI CACGTG 1 cut(s) 391
PmlI CACGTG 1 cut(s) 391
PpsI GAGTC 1 cut(s) 467
Ppu21I YACGTR 1 cut(s) 391
PsiI TTATAA 1 cut(s) 603
PspCI CACGTG 1 cut(s) 391
PspN4I GGNNCC 1 cut(s) 636
PspPI GGNCC 1 cut(s) 134
PsuI RGATCY 1 cut(s) 559
SapI GCTCTTC 1 cut(s) 516
SaqAI TTAA 4 cut(s) 35, 261, 269, 536
SatI GCNGC 4 cut(s) 66, 69, 108, 588
Sau3AI GATC 1 cut(s) 559
Sau96I GGNCC 1 cut(s) 134
SchI GAGTC 1 cut(s) 468
SduI GDGCHC 1 cut(s) 528
SetI ASST 8 cut(s) 43, 67, 208, 342, 358, 393, 448, 675
SfcI CTRYAG 1 cut(s) 165
SfuI TTCGAA 1 cut(s) 384
SinI GGWCC 1 cut(s) 134
Sse9I AATT 9 cut(s) 11, 87, 248, 266, 273, 434, 483, 579, 615
SsiI CCGC 5 cut(s) 105, 132, 142, 416, 490
TaaI ACNGT 2 cut(s) 184, 517
TaiI ACGT 1 cut(s) 393
TaqI TCGA 2 cut(s) 257, 384
TaqII GACCGA 2 cut(s) 674, 678
TasI AATT 9 cut(s) 11, 87, 248, 266, 273, 434, 483, 579, 615
Tru1I TTAA 4 cut(s) 35, 261, 269, 536
Tru9I TTAA 4 cut(s) 35, 261, 269, 536
TscAI CASTG 1 cut(s) 522
TseI GCWGC 4 cut(s) 65, 68, 107, 587
TspDTI ATGAA 2 cut(s) 17, 307
TspRI CASTG 1 cut(s) 522
VpaK11BI GGWCC 1 cut(s) 134
XmnI GAANNNNTTC 1 cut(s) 446
Zsp2I ATGCAT 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.