MD09G1011200.v1.1

Peptidase family M48

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0006709
Physical Location & Seq
Forward (+)
737125 .. 740343
3219 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1011200.v1.1.491

Sequence Viewer

Length: 1116 bp
ATGGCTTCCGCCGCCGCGCCCTGGATCTCTTCCCTCTGCCTGACGCAGAACCCTAGTTCTCTTAGTTTTAGCTCCGTGGCTTCGGATCATTTGCGGTTCGGTTCGCCCACCGTCGAATTCGCCGCCGTCAAGAAGTACCGGAGAGTTGGAATTAGAGGTCCTGTGTGCAAAGCTGCCTCCGTTGTGTTTAGCAACCTCGATGCCGATGATTTTCGGCACCCTCTGGATAAACAGAACACGCTGATTTTGAGAGCGATTCCGGGATTGAATGAGTTTGGAAAGATTCTGCTAGGATCTGTTGCAGAGCAAGTCATGCTTCTTGAGAACATCGGAACGTCCCTTCTTGTTTCGAAAGATCAGCTTTCTGGTCTACATCAGTTGATGGTGGAGGCCGCGGAGATTCTCAACATAGATGCTCCTGATTTATACGTTCGTCAAAGTCCTGTACCAAATGCGTATACTTTAGCAATAAGTGGTAAAAAGCCGTTTGTTGTTGTTCATACTAGCCTCGTGGAGCTTTTGACACGAGAAGAGCTGCAGGCTGTTTTGGCTCATGAGTTGGGTCATCTGAAATGCGACCATGGTGTGTGGCTTACATTTGCAAATATTCTTACCCTCGGAGCTTATACAATACCTGGGCTTGGTGGGATGATTGCTCAGAGGTTAGAAGAACAGTTATTTCGTTGGCTTCGAGCAGCAGAGCTAACTTGTGATCGTGCAGCTCTTCTTGTTGCACAAGACCCAAAGGTGGTCGTCTCTGTTCTAATGAAATTAGCTGGGGGCTGCCCATCTCTCGCTGATCAACTAAATGTGGATGCATTTTTGGAACAAGCTCGCTCCTACGACAAAGCTTCTTCAAACCCTGTGGGGTGGTATATAAGAAATGCTCAAACGAGGCAACTTTCACATCCTCTGCCGGTTCTACGTGCTCGTGAAATTGATGAATGGTCAAGAAGTCAAGATTACAAAACCCTTCTGAACCAGGCAACACGGGTCGCTGGTGCAAAGAAGGGATTAACAACACCTGGAAAGATAAAGTCAAAATCCATAGTACCCCCTCTGTGTGCTTCTCATGGCAAAGAACTCAGGGATGTGCTAAGACTTCATCTGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M48

Protein Analysis

372

Amino Acids

40.53

Weight (kDa)

8.96

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M48 PF01435 124 - 316 4.3e-18 Peptidase family M48
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 216
AccI GTMKAC 2 cut(s) 370, 458
AccII CGCG 2 cut(s) 17, 395
AciI CCGC 7 cut(s) 9, 12, 15, 94, 123, 393, 395
AclWI GGATC 3 cut(s) 32, 93, 301
AcsI RAATTY 1 cut(s) 116
AfaI GTAC 3 cut(s) 137, 447, 1053
AfiI CCNNNNNNNGG 3 cut(s) 21, 641, 748
AgsI TTSAA 2 cut(s) 268, 858
AjnI CCWGG 4 cut(s) 20, 634, 981, 1024
Alw21I GWGCWC 1 cut(s) 931
Alw26I GTCTC 1 cut(s) 760
AlwI GGATC 3 cut(s) 32, 93, 301
AoxI GGCC 1 cut(s) 390
ApeKI GCWGC 5 cut(s) 173, 535, 695, 719, 783
ApoI RAATTY 1 cut(s) 116
AspLEI GCGC 1 cut(s) 19
AspS9I GGNCC 1 cut(s) 158
AsuC2I CCSGG 1 cut(s) 261
AsuII TTCGAA 1 cut(s) 350
AvaII GGWCC 1 cut(s) 158
BanI GGYRCC 1 cut(s) 216
BauI CACGAG 3 cut(s) 509, 525, 930
Bbv12I GWGCWC 1 cut(s) 931
BbvI GCAGC 5 cut(s) 160, 522, 707, 731, 770
BccI CCATC 2 cut(s) 376, 796
BceAI ACGGC 2 cut(s) 110, 469
BciT130I CCWGG 4 cut(s) 22, 636, 983, 1026
BclI TGATCA 1 cut(s) 799
BcnI CCSGG 1 cut(s) 261
BcoDI GTCTC 1 cut(s) 760
BfaI CTAG 3 cut(s) 54, 290, 504
BfmI CTRYAG 1 cut(s) 536
BisI GCNGC 9 cut(s) 12, 15, 123, 174, 393, 536, 696, 720, 784
BlsI GCNGC 9 cut(s) 13, 16, 124, 175, 394, 537, 697, 721, 785
Bme1390I CCNGG 5 cut(s) 22, 261, 636, 983, 1026
Bme18I GGWCC 1 cut(s) 158
BmgT120I GGNCC 1 cut(s) 158
BmiI GGNNCC 1 cut(s) 218
BmrFI CCNGG 5 cut(s) 22, 261, 636, 983, 1026
BmsI GCATC 3 cut(s) 190, 403, 805
Bpu14I TTCGAA 1 cut(s) 350
BpuEI CTTGAG 1 cut(s) 341
BpuMI CCSGG 1 cut(s) 261
BsaAI YACGTR 1 cut(s) 926
BsaJI CCNNGG 6 cut(s) 20, 75, 393, 580, 616, 635
BsaWI WCCGGW 1 cut(s) 138
Bsc4I CCNNNNNNNGG 3 cut(s) 21, 641, 748
Bse118I RCCGGY 1 cut(s) 916
BseBI CCWGG 4 cut(s) 22, 636, 983, 1026
BseDI CCNNGG 6 cut(s) 20, 75, 393, 580, 616, 635
BseGI GGATG 4 cut(s) 654, 820, 907, 1096
BseLI CCNNNNNNNGG 3 cut(s) 21, 641, 748
BseMII CTCAG 2 cut(s) 671, 1099
BseXI GCAGC 5 cut(s) 160, 522, 707, 731, 770
BseYI CCCAGC 1 cut(s) 776
BsgI GTGCAG 1 cut(s) 738
Bsh1236I CGCG 2 cut(s) 17, 395
BshFI GGCC 1 cut(s) 392
BshNI GGYRCC 1 cut(s) 216
BsiHKAI GWGCWC 1 cut(s) 931
BsiSI CCGG 3 cut(s) 139, 260, 917
BslFI GGGAC 1 cut(s) 322
BslI CCNNNNNNNGG 3 cut(s) 21, 641, 748
BsmAI GTCTC 1 cut(s) 760
BsmBI CGTCTC 1 cut(s) 760
BsmFI GGGAC 1 cut(s) 322
BsnI GGCC 1 cut(s) 392
Bsp119I TTCGAA 1 cut(s) 350
Bsp1286I GDGCHC 1 cut(s) 931
Bsp143I GATC 6 cut(s) 24, 85, 293, 355, 712, 799
Bsp19I CCATGG 1 cut(s) 580
BspACI CCGC 7 cut(s) 9, 12, 15, 94, 123, 393, 395
BspANI GGCC 1 cut(s) 392
BspCNI CTCAG 2 cut(s) 670, 1098
BspFNI CGCG 2 cut(s) 17, 395
BspHI TCATGA 1 cut(s) 553
BspLI GGNNCC 1 cut(s) 218
BspMAI CTGCAG 1 cut(s) 540
BspPI GGATC 3 cut(s) 32, 93, 301
BspQI GCTCTTC 2 cut(s) 525, 729
BspT104I TTCGAA 1 cut(s) 350
BspT107I GGYRCC 1 cut(s) 216
BsrFI RCCGGY 1 cut(s) 916
BssAI RCCGGY 1 cut(s) 916
BssECI CCNNGG 6 cut(s) 20, 75, 393, 580, 616, 635
BssMI GATC 6 cut(s) 24, 85, 293, 355, 712, 799
BssNAI GTATAC 1 cut(s) 459
BssSI CACGAG 3 cut(s) 509, 525, 930
BssT1I CCWWGG 1 cut(s) 580
Bst1107I GTATAC 1 cut(s) 459
Bst2BI CACGAG 3 cut(s) 509, 525, 930
Bst2UI CCWGG 4 cut(s) 22, 636, 983, 1026
Bst4CI ACNGT 2 cut(s) 112, 675
Bst6I CTCTTC 3 cut(s) 34, 525, 729
BstAPI GCANNNNNTGC 1 cut(s) 313
BstBAI YACGTR 1 cut(s) 926
BstBI TTCGAA 1 cut(s) 350
BstC8I GCNNGC 2 cut(s) 540, 835
BstDEI CTNAG 4 cut(s) 62, 657, 1085, 1097
BstDSI CCRYGG 3 cut(s) 75, 393, 580
BstF5I GGATG 4 cut(s) 654, 820, 907, 1096
BstFNI CGCG 2 cut(s) 17, 395
BstHHI GCGC 1 cut(s) 19
BstKTI GATC 6 cut(s) 27, 88, 296, 358, 715, 802
BstMAI GTCTC 1 cut(s) 760
BstMBI GATC 6 cut(s) 24, 85, 293, 355, 712, 799
BstMWI GCNNNNNNNGC 3 cut(s) 11, 313, 548
BstNI CCWGG 4 cut(s) 22, 636, 983, 1026
BstSCI CCNGG 5 cut(s) 20, 259, 634, 981, 1024
BstSFI CTRYAG 1 cut(s) 536
BstUI CGCG 2 cut(s) 17, 395
BstV1I GCAGC 5 cut(s) 160, 522, 707, 731, 770
BstX2I RGATCY 2 cut(s) 24, 293
BstYI RGATCY 2 cut(s) 24, 293
BstZ17I GTATAC 1 cut(s) 459
BsuRI GGCC 1 cut(s) 392
BtgI CCRYGG 3 cut(s) 75, 393, 580
BtsCI GGATG 4 cut(s) 654, 820, 907, 1096
Cac8I GCNNGC 2 cut(s) 540, 835
CciI TCATGA 1 cut(s) 553
CfoI GCGC 1 cut(s) 19
Cfr10I RCCGGY 1 cut(s) 916
Cfr13I GGNCC 1 cut(s) 158
Cfr42I CCGCGG 1 cut(s) 396
CseI GACGC 1 cut(s) 52
Csp6I GTAC 3 cut(s) 136, 446, 1052
CspCI CAANNNNNGTGG 2 cut(s) 846, 881
CviAII CATG 4 cut(s) 313, 554, 581, 1073
CviQI GTAC 3 cut(s) 136, 446, 1052
DdeI CTNAG 4 cut(s) 62, 657, 1085, 1097
DpnI GATC 6 cut(s) 26, 87, 295, 357, 714, 801
DpnII GATC 6 cut(s) 24, 85, 293, 355, 712, 799
Eam1104I CTCTTC 3 cut(s) 34, 525, 729
EarI CTCTTC 3 cut(s) 34, 525, 729
Eco130I CCWWGG 1 cut(s) 580
Eco47I GGWCC 1 cut(s) 158
EcoO109I RGGNCCY 1 cut(s) 158
EcoRI GAATTC 1 cut(s) 116
EcoRII CCWGG 4 cut(s) 20, 634, 981, 1024
EcoT14I CCWWGG 1 cut(s) 580
EcoT22I ATGCAT 1 cut(s) 820
ErhI CCWWGG 1 cut(s) 580
Esp3I CGTCTC 1 cut(s) 760
FaeI CATG 4 cut(s) 316, 557, 584, 1076
FalI AAGNNNNNCTT 4 cut(s) 300, 332, 345, 377
FaqI GGGAC 1 cut(s) 322
FatI CATG 4 cut(s) 312, 553, 580, 1072
FbaI TGATCA 1 cut(s) 799
FblI GTMKAC 2 cut(s) 370, 458
Fnu4HI GCNGC 9 cut(s) 12, 15, 123, 174, 393, 536, 696, 720, 784
FokI GGATG 4 cut(s) 661, 827, 894, 1103
Fsp4HI GCNGC 9 cut(s) 12, 15, 123, 174, 393, 536, 696, 720, 784
FspBI CTAG 3 cut(s) 54, 290, 504
GlaI GCGC 1 cut(s) 18
GluI GCNGC 9 cut(s) 12, 15, 123, 174, 393, 536, 696, 720, 784
GsaI CCCAGC 1 cut(s) 780
HaeIII GGCC 1 cut(s) 392
HapII CCGG 3 cut(s) 139, 260, 917
HgaI GACGC 1 cut(s) 52
HhaI GCGC 1 cut(s) 19
Hin1II CATG 4 cut(s) 316, 557, 584, 1076
Hin6I GCGC 1 cut(s) 17
HinP1I GCGC 1 cut(s) 17
HindIII AAGCTT 1 cut(s) 849
HinfI GANTC 3 cut(s) 256, 283, 400
HpaII CCGG 3 cut(s) 139, 260, 917
Hpy166II GTNNAC 2 cut(s) 371, 459
Hpy188I TCNGA 6 cut(s) 85, 332, 570, 620, 660, 978
Hpy188III TCNNGA 8 cut(s) 130, 224, 320, 419, 554, 932, 951, 959
Hpy8I GTNNAC 2 cut(s) 371, 459
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 3 cut(s) 350, 983, 1003
HpyCH4III ACNGT 2 cut(s) 112, 675
HpyCH4IV ACGT 3 cut(s) 335, 429, 925
HpyCH4V TGCA 8 cut(s) 168, 302, 538, 602, 719, 734, 818, 1004
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 313, 548
HpyF3I CTNAG 4 cut(s) 62, 657, 1085, 1097
HpySE526I ACGT 3 cut(s) 335, 429, 925
Hsp92II CATG 4 cut(s) 316, 557, 584, 1076
HspAI GCGC 1 cut(s) 17
Ksp22I TGATCA 1 cut(s) 799
KspI CCGCGG 1 cut(s) 396
Kzo9I GATC 6 cut(s) 24, 85, 293, 355, 712, 799
LguI GCTCTTC 2 cut(s) 525, 729
LmnI GCTCC 5 cut(s) 77, 421, 514, 620, 842
Lsp1109I GCAGC 5 cut(s) 160, 522, 707, 731, 770
LweI GCATC 3 cut(s) 190, 403, 805
MaeI CTAG 3 cut(s) 54, 290, 504
MaeII ACGT 3 cut(s) 335, 429, 925
MalI GATC 6 cut(s) 26, 87, 295, 357, 714, 801
MboI GATC 6 cut(s) 24, 85, 293, 355, 712, 799
MboII GAAGA 5 cut(s) 21, 542, 680, 716, 846
MflI RGATCY 2 cut(s) 24, 293
MhlI GDGCHC 1 cut(s) 931
MluCI AATT 4 cut(s) 116, 150, 770, 936
MmeI TCCRAC 1 cut(s) 127
Mph1103I ATGCAT 1 cut(s) 820
MseI TTAA 2 cut(s) 1016, 1114
MspA1I CMGCKG 1 cut(s) 395
MspI CCGG 3 cut(s) 139, 260, 917
MspR9I CCNGG 5 cut(s) 22, 261, 636, 983, 1026
MvaI CCWGG 4 cut(s) 22, 636, 983, 1026
MvnI CGCG 2 cut(s) 17, 395
MwoI GCNNNNNNNGC 3 cut(s) 11, 313, 548
NciI CCSGG 1 cut(s) 261
NcoI CCATGG 1 cut(s) 580
NdeII GATC 6 cut(s) 24, 85, 293, 355, 712, 799
NlaIII CATG 4 cut(s) 316, 557, 584, 1076
NlaIV GGNNCC 1 cut(s) 218
NsiI ATGCAT 1 cut(s) 820
NspV TTCGAA 1 cut(s) 350
PagI TCATGA 1 cut(s) 553
PciSI GCTCTTC 2 cut(s) 525, 729
PcsI WCGNNNNNNNCGW 1 cut(s) 688
PfeI GAWTC 3 cut(s) 256, 283, 400
PfoI TCCNGGA 1 cut(s) 259
PkrI GCNGC 9 cut(s) 13, 16, 124, 175, 394, 537, 697, 721, 785
Ppu21I YACGTR 1 cut(s) 926
PpuMI RGGWCCY 1 cut(s) 158
Psp5II RGGWCCY 1 cut(s) 158
Psp6I CCWGG 4 cut(s) 20, 634, 981, 1024
PspFI CCCAGC 1 cut(s) 776
PspGI CCWGG 4 cut(s) 20, 634, 981, 1024
PspN4I GGNNCC 1 cut(s) 218
PspPI GGNCC 1 cut(s) 158
PspPPI RGGWCCY 1 cut(s) 158
PstI CTGCAG 1 cut(s) 540
PsuI RGATCY 2 cut(s) 24, 293
RsaI GTAC 3 cut(s) 137, 447, 1053
RsaNI GTAC 3 cut(s) 136, 446, 1052
SacII CCGCGG 1 cut(s) 396
SapI GCTCTTC 2 cut(s) 525, 729
SaqAI TTAA 2 cut(s) 1016, 1114
SatI GCNGC 9 cut(s) 12, 15, 123, 174, 393, 536, 696, 720, 784
Sau3AI GATC 6 cut(s) 24, 85, 293, 355, 712, 799
Sau96I GGNCC 1 cut(s) 158
ScrFI CCNGG 5 cut(s) 22, 261, 636, 983, 1026
SduI GDGCHC 1 cut(s) 931
SfaNI GCATC 3 cut(s) 190, 403, 805
SfcI CTRYAG 1 cut(s) 536
Sfr303I CCGCGG 1 cut(s) 396
SfuI TTCGAA 1 cut(s) 350
SgrBI CCGCGG 1 cut(s) 396
SinI GGWCC 1 cut(s) 158
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 4 cut(s) 116, 150, 770, 936
SsiI CCGC 7 cut(s) 9, 12, 15, 94, 123, 393, 395
SspI AATATT 1 cut(s) 607
SspMI CTAG 3 cut(s) 54, 290, 504
StyD4I CCNGG 5 cut(s) 20, 259, 634, 981, 1024
StyI CCWWGG 1 cut(s) 580
TaaI ACNGT 2 cut(s) 112, 675
TaiI ACGT 3 cut(s) 338, 432, 928
TaqI TCGA 4 cut(s) 114, 198, 350, 691
TasI AATT 4 cut(s) 116, 150, 770, 936
TauI GCSGC 4 cut(s) 14, 17, 125, 395
TfiI GAWTC 3 cut(s) 256, 283, 400
Tru1I TTAA 2 cut(s) 1016, 1114
Tru9I TTAA 2 cut(s) 1016, 1114
TseI GCWGC 5 cut(s) 173, 535, 695, 719, 783
TspDTI ATGAA 4 cut(s) 488, 782, 957, 1094
TspGWI ACGGA 2 cut(s) 64, 169
VpaK11BI GGWCC 1 cut(s) 158
XapI RAATTY 1 cut(s) 116
XmiI GTMKAC 2 cut(s) 370, 458
XspI CTAG 3 cut(s) 54, 290, 504
Zsp2I ATGCAT 1 cut(s) 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.