Rh2BG675800

Peptidase family M48

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0006709
Physical Location & Seq
Reverse (-)
88706386 .. 88709655
3270 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG675800.1

Sequence Viewer

Length: 921 bp
ATGCGTATCATGACTAGAAGATGGGATATTACTGAAACCATAACACTTGGAGAGTTTTTCTCTCTTCCATCCACGAGCTTCGCCGCCTTTATTGCCCTAGCGGTGGTTGGTGGAGCACCCGGTCTCGGCAAGATCGGTGAAAGGCCTATTCGGAAGTCTTCCAAATCCTATCTTCTTGGATCTGTTACGGAGCAAGTGATGCTTCTTGAGAATATCGGAACGTCCCTTCTTGTTTCGGAAAATCAGCTTTCTGATCTACATAAATTGATGGTTGAAGCCGCGGACATACTCAACATTGATGCTCCTGATCTATATGTTCGTCAAAGTCCTGTACCAAATGCATACACTTTAGCAATAAGTGGTAAAAAGCCATTTGTTGTTATCCATACTAGTCTAGTGGAGCTTTTGACACGAGAAGAGTTGCAGGCTGTTTTGGCTCATGAGTTGGGTCATTTGAAATGCGACCATGGTGTGTGGCTTACATTTGCAAATATCCTTACCCTTGGAGCTTATACCATACCTGGGCTTGGTGGCATGATTGCTCAGAGTTTAGAAGAGCAGTTATTTCGTTGGCTTCGAGCAGCAGAGCTAACCTGTGATCGTGCAGCCCTTCTTGTTGCTCAAGACCCAAAGGTGGTCGTCTCTGTGTTGATGAAACTAGCTGGTGGATGCCCATCTATGGCTGATCAACTAAATGTGGATGCATTTTTGGAACAAGCTCGCTCTTATGACAAAGCTTCTTCAAGCCCTGTAGGCTGGTATATAAGAAATGCACAAACGAGGCAACTTTCACATCCTTTGCCGGTACTACGAGCTCGTGAAATTGATACATGGTCAAGAAGTCAAGAGTACAGAACTCTTTTAAAACGAGCAACACAGATCACTGCTGGATCAAAAATTTTAACAACACCTGGAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M48

Protein Analysis

306

Amino Acids

33.62

Weight (kDa)

8.4

Isoelectric Point (pI)

50.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M48 PF01435 84 - 278 2.1e-18 Peptidase family M48
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 281
AciI CCGC 4 cut(s) 84, 101, 279, 281
AclWI GGATC 2 cut(s) 187, 898
AcsI RAATTY 1 cut(s) 897
AfaI GTAC 3 cut(s) 333, 807, 851
AfiI CCNNNNNNNGG 6 cut(s) 103, 125, 522, 527, 634, 679
AgsI TTSAA 3 cut(s) 275, 457, 744
AhlI ACTAGT 1 cut(s) 389
AjnI CCWGG 2 cut(s) 520, 910
AluBI AGCT 9 cut(s) 78, 247, 403, 509, 589, 662, 719, 737, 815
AluI AGCT 9 cut(s) 78, 247, 403, 509, 589, 662, 719, 737, 815
Alw21I GWGCWC 2 cut(s) 118, 817
Alw26I GTCTC 2 cut(s) 128, 646
AlwI GGATC 2 cut(s) 187, 898
AoxI GGCC 1 cut(s) 143
ApeKI GCWGC 2 cut(s) 581, 605
ApoI RAATTY 1 cut(s) 897
AsuC2I CCSGG 1 cut(s) 120
AsuHPI GGTGA 1 cut(s) 149
BanII GRGCYC 1 cut(s) 817
BauI CACGAG 3 cut(s) 73, 411, 816
BbsI GAAGAC 1 cut(s) 150
Bbv12I GWGCWC 2 cut(s) 118, 817
BbvI GCAGC 2 cut(s) 593, 617
BccI CCATC 4 cut(s) 15, 76, 262, 682
BcgI CGANNNNNNTGC 2 cut(s) 548, 582
BciT130I CCWGG 2 cut(s) 522, 912
BclI TGATCA 1 cut(s) 685
BcnI CCSGG 1 cut(s) 120
BcoDI GTCTC 2 cut(s) 128, 646
BcuI ACTAGT 1 cut(s) 389
BfaI CTAG 5 cut(s) 15, 98, 390, 395, 659
BfmI CTRYAG 1 cut(s) 750
BglI GCCNNNNNGGC 1 cut(s) 753
BisI GCNGC 4 cut(s) 84, 279, 582, 606
BlsI GCNGC 4 cut(s) 85, 280, 583, 607
Bme1390I CCNGG 3 cut(s) 120, 522, 912
BmrFI CCNGG 3 cut(s) 120, 522, 912
BmsI GCATC 4 cut(s) 189, 289, 659, 691
BpiI GAAGAC 1 cut(s) 150
BplI GAGNNNNNCTC 2 cut(s) 44, 76
BpuEI CTTGAG 2 cut(s) 227, 606
BpuMI CCSGG 1 cut(s) 120
BsaBI GATNNNNATC 1 cut(s) 673
BsaI GGTCTC 1 cut(s) 128
BsaJI CCNNGG 4 cut(s) 279, 466, 502, 521
Bsc4I CCNNNNNNNGG 6 cut(s) 103, 125, 522, 527, 634, 679
Bse118I RCCGGY 1 cut(s) 802
Bse8I GATNNNNATC 1 cut(s) 673
BseBI CCWGG 2 cut(s) 522, 912
BseDI CCNNGG 4 cut(s) 279, 466, 502, 521
BseGI GGATG 4 cut(s) 68, 674, 706, 793
BseJI GATNNNNATC 1 cut(s) 673
BseLI CCNNNNNNNGG 6 cut(s) 103, 125, 522, 527, 634, 679
BseMII CTCAG 1 cut(s) 557
BseXI GCAGC 2 cut(s) 593, 617
BsgI GTGCAG 1 cut(s) 624
Bsh1236I CGCG 1 cut(s) 281
BshFI GGCC 1 cut(s) 145
BsiHKAI GWGCWC 2 cut(s) 118, 817
BsiSI CCGG 2 cut(s) 120, 803
BslFI GGGAC 1 cut(s) 208
BslI CCNNNNNNNGG 6 cut(s) 103, 125, 522, 527, 634, 679
BsmAI GTCTC 2 cut(s) 128, 646
BsmBI CGTCTC 1 cut(s) 646
BsmFI GGGAC 1 cut(s) 208
BsnI GGCC 1 cut(s) 145
Bso31I GGTCTC 1 cut(s) 128
Bsp1286I GDGCHC 2 cut(s) 118, 817
Bsp143I GATC 8 cut(s) 132, 179, 253, 307, 598, 685, 879, 890
Bsp19I CCATGG 1 cut(s) 466
BspACI CCGC 4 cut(s) 84, 101, 279, 281
BspANI GGCC 1 cut(s) 145
BspCNI CTCAG 1 cut(s) 556
BspFNI CGCG 1 cut(s) 281
BspHI TCATGA 2 cut(s) 9, 439
BspPI GGATC 2 cut(s) 187, 898
BspQI GCTCTTC 1 cut(s) 549
BspTNI GGTCTC 1 cut(s) 128
BsrFI RCCGGY 1 cut(s) 802
BssAI RCCGGY 1 cut(s) 802
BssECI CCNNGG 4 cut(s) 279, 466, 502, 521
BssMI GATC 8 cut(s) 132, 179, 253, 307, 598, 685, 879, 890
BssSI CACGAG 3 cut(s) 73, 411, 816
BssT1I CCWWGG 2 cut(s) 466, 502
Bst2BI CACGAG 3 cut(s) 73, 411, 816
Bst2UI CCWGG 2 cut(s) 522, 912
Bst6I CTCTTC 3 cut(s) 69, 411, 549
BstAPI GCANNNNNTGC 1 cut(s) 199
BstC8I GCNNGC 2 cut(s) 426, 721
BstDEI CTNAG 1 cut(s) 543
BstDSI CCRYGG 2 cut(s) 279, 466
BstF5I GGATG 4 cut(s) 68, 674, 706, 793
BstFNI CGCG 1 cut(s) 281
BstKTI GATC 8 cut(s) 135, 182, 256, 310, 601, 688, 882, 893
BstMAI GTCTC 2 cut(s) 128, 646
BstMBI GATC 8 cut(s) 132, 179, 253, 307, 598, 685, 879, 890
BstMWI GCNNNNNNNGC 4 cut(s) 92, 199, 434, 753
BstNI CCWGG 2 cut(s) 522, 912
BstSCI CCNGG 3 cut(s) 118, 520, 910
BstSFI CTRYAG 1 cut(s) 750
BstUI CGCG 1 cut(s) 281
BstV1I GCAGC 2 cut(s) 593, 617
BstV2I GAAGAC 1 cut(s) 150
BstX2I RGATCY 1 cut(s) 179
BstYI RGATCY 1 cut(s) 179
BsuRI GGCC 1 cut(s) 145
BtgI CCRYGG 2 cut(s) 279, 466
BtsCI GGATG 4 cut(s) 68, 674, 706, 793
BtsI GCAGTG 1 cut(s) 882
BtsIMutI CAGTG 1 cut(s) 882
Cac8I GCNNGC 2 cut(s) 426, 721
CciI TCATGA 2 cut(s) 9, 439
Cfr10I RCCGGY 1 cut(s) 802
Cfr42I CCGCGG 1 cut(s) 282
Csp6I GTAC 3 cut(s) 332, 806, 850
CviAII CATG 5 cut(s) 10, 440, 467, 535, 831
CviQI GTAC 3 cut(s) 332, 806, 850
DdeI CTNAG 1 cut(s) 543
DpnI GATC 8 cut(s) 134, 181, 255, 309, 600, 687, 881, 892
DpnII GATC 8 cut(s) 132, 179, 253, 307, 598, 685, 879, 890
DraI TTTAAA 1 cut(s) 864
Eam1104I CTCTTC 3 cut(s) 69, 411, 549
EarI CTCTTC 3 cut(s) 69, 411, 549
Ecl136II GAGCTC 1 cut(s) 815
Eco130I CCWWGG 2 cut(s) 466, 502
Eco147I AGGCCT 1 cut(s) 145
Eco24I GRGCYC 1 cut(s) 817
Eco31I GGTCTC 1 cut(s) 128
Eco53kI GAGCTC 1 cut(s) 815
EcoICRI GAGCTC 1 cut(s) 815
EcoRII CCWGG 2 cut(s) 520, 910
EcoT14I CCWWGG 2 cut(s) 466, 502
EcoT22I ATGCAT 2 cut(s) 343, 706
EcoT38I GRGCYC 1 cut(s) 817
ErhI CCWWGG 2 cut(s) 466, 502
Esp3I CGTCTC 1 cut(s) 646
FaeI CATG 5 cut(s) 13, 443, 470, 538, 834
FalI AAGNNNNNCTT 2 cut(s) 186, 218
FaqI GGGAC 1 cut(s) 208
FatI CATG 5 cut(s) 9, 439, 466, 534, 830
FbaI TGATCA 1 cut(s) 685
Fnu4HI GCNGC 4 cut(s) 84, 279, 582, 606
FokI GGATG 4 cut(s) 55, 681, 713, 780
FriOI GRGCYC 1 cut(s) 817
Fsp4HI GCNGC 4 cut(s) 84, 279, 582, 606
FspBI CTAG 5 cut(s) 15, 98, 390, 395, 659
GluI GCNGC 4 cut(s) 84, 279, 582, 606
HaeIII GGCC 1 cut(s) 145
HapII CCGG 2 cut(s) 120, 803
Hin1II CATG 5 cut(s) 13, 443, 470, 538, 834
HindIII AAGCTT 1 cut(s) 735
HpaII CCGG 2 cut(s) 120, 803
HphI GGTGA 1 cut(s) 149
Hpy188I TCNGA 5 cut(s) 153, 218, 238, 253, 546
Hpy188III TCNNGA 8 cut(s) 10, 206, 305, 440, 623, 818, 837, 845
HpyAV CCTTC 2 cut(s) 236, 620
HpyCH4IV ACGT 1 cut(s) 221
HpyCH4V TGCA 6 cut(s) 341, 424, 488, 605, 704, 773
HpyF10VI GCNNNNNNNGC 4 cut(s) 92, 199, 434, 753
HpyF3I CTNAG 1 cut(s) 543
HpySE526I ACGT 1 cut(s) 221
Hsp92II CATG 5 cut(s) 13, 443, 470, 538, 834
Ksp22I TGATCA 1 cut(s) 685
KspI CCGCGG 1 cut(s) 282
Kzo9I GATC 8 cut(s) 132, 179, 253, 307, 598, 685, 879, 890
LguI GCTCTTC 1 cut(s) 549
LmnI GCTCC 5 cut(s) 113, 190, 307, 400, 506
Lsp1109I GCAGC 2 cut(s) 593, 617
LweI GCATC 4 cut(s) 189, 289, 659, 691
MaeI CTAG 5 cut(s) 15, 98, 390, 395, 659
MaeII ACGT 1 cut(s) 221
MaeIII GTNAC 1 cut(s) 184
MalI GATC 8 cut(s) 134, 181, 255, 309, 600, 687, 881, 892
MboI GATC 8 cut(s) 132, 179, 253, 307, 598, 685, 879, 890
MboII GAAGA 7 cut(s) 30, 56, 150, 164, 428, 566, 732
MflI RGATCY 1 cut(s) 179
MhlI GDGCHC 2 cut(s) 118, 817
MluCI AATT 3 cut(s) 263, 822, 897
MnlI CCTC 1 cut(s) 774
Mph1103I ATGCAT 2 cut(s) 343, 706
MseI TTAA 2 cut(s) 863, 902
MspA1I CMGCKG 1 cut(s) 281
MspI CCGG 2 cut(s) 120, 803
MspR9I CCNGG 3 cut(s) 120, 522, 912
MvaI CCWGG 2 cut(s) 522, 912
MvnI CGCG 1 cut(s) 281
MwoI GCNNNNNNNGC 4 cut(s) 92, 199, 434, 753
NciI CCSGG 1 cut(s) 120
NcoI CCATGG 1 cut(s) 466
NdeII GATC 8 cut(s) 132, 179, 253, 307, 598, 685, 879, 890
NlaIII CATG 5 cut(s) 13, 443, 470, 538, 834
NmeAIII GCCGAG 1 cut(s) 105
NsiI ATGCAT 2 cut(s) 343, 706
PagI TCATGA 2 cut(s) 9, 439
PceI AGGCCT 1 cut(s) 145
PciSI GCTCTTC 1 cut(s) 549
PcsI WCGNNNNNNNCGW 1 cut(s) 574
PkrI GCNGC 4 cut(s) 85, 280, 583, 607
Psp124BI GAGCTC 1 cut(s) 817
Psp6I CCWGG 2 cut(s) 520, 910
PspGI CCWGG 2 cut(s) 520, 910
PsuI RGATCY 1 cut(s) 179
RsaI GTAC 3 cut(s) 333, 807, 851
RsaNI GTAC 3 cut(s) 332, 806, 850
SacI GAGCTC 1 cut(s) 817
SacII CCGCGG 1 cut(s) 282
SapI GCTCTTC 1 cut(s) 549
SaqAI TTAA 2 cut(s) 863, 902
SatI GCNGC 4 cut(s) 84, 279, 582, 606
Sau3AI GATC 8 cut(s) 132, 179, 253, 307, 598, 685, 879, 890
ScrFI CCNGG 3 cut(s) 120, 522, 912
SduI GDGCHC 2 cut(s) 118, 817
SfaNI GCATC 4 cut(s) 189, 289, 659, 691
SfcI CTRYAG 1 cut(s) 750
Sfr303I CCGCGG 1 cut(s) 282
SgrBI CCGCGG 1 cut(s) 282
SmlI CTYRAG 2 cut(s) 206, 621
SmoI CTYRAG 2 cut(s) 206, 621
SpeI ACTAGT 1 cut(s) 389
Sse9I AATT 3 cut(s) 263, 822, 897
SseBI AGGCCT 1 cut(s) 145
SsiI CCGC 4 cut(s) 84, 101, 279, 281
SspMI CTAG 5 cut(s) 15, 98, 390, 395, 659
SstI GAGCTC 1 cut(s) 817
StuI AGGCCT 1 cut(s) 145
StyD4I CCNGG 3 cut(s) 118, 520, 910
StyI CCWWGG 2 cut(s) 466, 502
TaiI ACGT 1 cut(s) 224
TaqI TCGA 1 cut(s) 577
TasI AATT 3 cut(s) 263, 822, 897
TatI WGTACW 1 cut(s) 849
TauI GCSGC 2 cut(s) 86, 281
Tru1I TTAA 2 cut(s) 863, 902
Tru9I TTAA 2 cut(s) 863, 902
TscAI CASTG 1 cut(s) 889
TseI GCWGC 2 cut(s) 581, 605
TspDTI ATGAA 1 cut(s) 668
TspGWI ACGGA 1 cut(s) 203
TspRI CASTG 1 cut(s) 889
XapI RAATTY 1 cut(s) 897
XspI CTAG 5 cut(s) 15, 98, 390, 395, 659
Zsp2I ATGCAT 2 cut(s) 343, 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.