pycom111g00950

Peptidase family M48

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0006709
Physical Location & Seq
Forward (+)
610543 .. 612177
1635 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g00950.3

Sequence Viewer

Length: 768 bp
ATGGCTTCCGCTGCCGCGCCCTTGATCTCTTCTCTCTGCCTAACACAGAACCTTAGTTCTCTTAGTTTTAGCTCCGTGGCTTCGGATCATTTGCGGTTCGGTTCGCCCACCGTCGAATTCGCCGCCGTCAAGAAGTACCGGAGAGTTGGAATTCGAGTTCCTGTGTGCAGAGCTGCCTCCGTTGTGTTTAGCAACCTCGATGCCGATGATTTTCGGCACCCTTTGGATAAACAGAACACGCTGATTTTGAGAGCGATTCCGGGATTGAATGAGTTTGGAAAGATTCTGCTAGGATCTGTTGCAGAGCAAGTCATGCTTCTTGAGAACATCGGAACGTCCCTTCTTGTTTCGAAAGATCAGCTTTCTGGCCTACATCAATTGATGGTGGAGGCTGCGGAGATTCTCAGCATAGATGCTCCTGATTTATATGTTCGTCAAAGTCCTGTACCAAATGCGTATACTTTAGCAATAAGTGGTAAAAAGCCGTTTGTTGTTGTTCATACTAGTCTCGTGGAGCTTTTGACACAAGAAGAGCTGCAGGCTGTTTTGGCTCATGAGTTGGGTCATTTGAAATGCGACCATGGTGTGTGGCTTACGTTTGCAAATATTCTTACCCTTGGAGCTTATACAATACCTGGGCTAGGTGGGATGATTGCTCAGAGGTTGGAAGAACAGTTATTTCGTTGGCTTCGAGCAGCAGAGCTAACTTGTGATCGTGCAGCTCTTCTTGTTGCACAAAACCCAAAGAGGTGGTCGTCTCTGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M48

Protein Analysis

256

Amino Acids

27.83

Weight (kDa)

6.65

Isoelectric Point (pI)

45.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 216
AccI GTMKAC 1 cut(s) 458
AccII CGCG 1 cut(s) 17
AciI CCGC 5 cut(s) 9, 15, 94, 123, 395
AclWI GGATC 2 cut(s) 93, 301
AcsI RAATTY 2 cut(s) 116, 150
AfaI GTAC 2 cut(s) 137, 447
AfiI CCNNNNNNNGG 1 cut(s) 641
AgsI TTSAA 2 cut(s) 268, 571
AhlI ACTAGT 1 cut(s) 503
AjnI CCWGG 1 cut(s) 634
AloI GAACNNNNNNTCC 2 cut(s) 141, 173
AluBI AGCT 8 cut(s) 72, 173, 361, 517, 535, 623, 703, 722
AluI AGCT 8 cut(s) 72, 173, 361, 517, 535, 623, 703, 722
Alw26I GTCTC 2 cut(s) 512, 762
AlwI GGATC 2 cut(s) 93, 301
AoxI GGCC 1 cut(s) 367
ApeKI GCWGC 6 cut(s) 11, 173, 392, 535, 695, 719
ApoI RAATTY 2 cut(s) 116, 150
ArsI GACNNNNNNTTYG 1 cut(s) 737
AspLEI GCGC 1 cut(s) 19
AsuC2I CCSGG 1 cut(s) 261
AsuII TTCGAA 1 cut(s) 350
BanI GGYRCC 1 cut(s) 216
BauI CACGAG 1 cut(s) 509
BbvI GCAGC 5 cut(s) 160, 379, 522, 707, 731
BccI CCATC 1 cut(s) 376
BceAI ACGGC 2 cut(s) 110, 469
BciT130I CCWGG 1 cut(s) 636
BcnI CCSGG 1 cut(s) 261
BcoDI GTCTC 2 cut(s) 512, 762
BcuI ACTAGT 1 cut(s) 503
BfaI CTAG 3 cut(s) 290, 504, 641
BfmI CTRYAG 1 cut(s) 536
BisI GCNGC 8 cut(s) 12, 15, 123, 174, 393, 536, 696, 720
BlsI GCNGC 8 cut(s) 13, 16, 124, 175, 394, 537, 697, 721
Bme1390I CCNGG 2 cut(s) 261, 636
BmiI GGNNCC 1 cut(s) 218
BmrFI CCNGG 2 cut(s) 261, 636
BmsI GCATC 2 cut(s) 190, 403
Bpu14I TTCGAA 1 cut(s) 350
BpuEI CTTGAG 1 cut(s) 341
BpuMI CCSGG 1 cut(s) 261
BsaJI CCNNGG 4 cut(s) 75, 580, 616, 635
BsaWI WCCGGW 1 cut(s) 138
Bsc4I CCNNNNNNNGG 1 cut(s) 641
BseBI CCWGG 1 cut(s) 636
BseDI CCNNGG 4 cut(s) 75, 580, 616, 635
BseGI GGATG 1 cut(s) 654
BseLI CCNNNNNNNGG 1 cut(s) 641
BseMII CTCAG 2 cut(s) 418, 671
BseXI GCAGC 5 cut(s) 160, 379, 522, 707, 731
BsgI GTGCAG 2 cut(s) 187, 738
Bsh1236I CGCG 1 cut(s) 17
BshFI GGCC 1 cut(s) 369
BshNI GGYRCC 1 cut(s) 216
BsiSI CCGG 2 cut(s) 139, 260
BslFI GGGAC 1 cut(s) 322
BslI CCNNNNNNNGG 1 cut(s) 641
BsmAI GTCTC 2 cut(s) 512, 762
BsmBI CGTCTC 1 cut(s) 762
BsmFI GGGAC 1 cut(s) 322
BsnI GGCC 1 cut(s) 369
Bsp119I TTCGAA 1 cut(s) 350
Bsp143I GATC 5 cut(s) 24, 85, 293, 355, 712
Bsp19I CCATGG 1 cut(s) 580
BspACI CCGC 5 cut(s) 9, 15, 94, 123, 395
BspANI GGCC 1 cut(s) 369
BspCNI CTCAG 2 cut(s) 417, 670
BspFNI CGCG 1 cut(s) 17
BspHI TCATGA 1 cut(s) 553
BspLI GGNNCC 1 cut(s) 218
BspMAI CTGCAG 1 cut(s) 540
BspPI GGATC 2 cut(s) 93, 301
BspQI GCTCTTC 2 cut(s) 525, 729
BspT104I TTCGAA 1 cut(s) 350
BspT107I GGYRCC 1 cut(s) 216
BssECI CCNNGG 4 cut(s) 75, 580, 616, 635
BssMI GATC 5 cut(s) 24, 85, 293, 355, 712
BssNAI GTATAC 1 cut(s) 459
BssSI CACGAG 1 cut(s) 509
BssT1I CCWWGG 2 cut(s) 580, 616
Bst1107I GTATAC 1 cut(s) 459
Bst2BI CACGAG 1 cut(s) 509
Bst2UI CCWGG 1 cut(s) 636
Bst4CI ACNGT 2 cut(s) 112, 675
Bst6I CTCTTC 3 cut(s) 34, 525, 729
BstAPI GCANNNNNTGC 1 cut(s) 313
BstBI TTCGAA 1 cut(s) 350
BstC8I GCNNGC 1 cut(s) 540
BstDEI CTNAG 4 cut(s) 53, 62, 404, 657
BstDSI CCRYGG 2 cut(s) 75, 580
BstENI CCTNNNNNAGG 1 cut(s) 639
BstF5I GGATG 1 cut(s) 654
BstFNI CGCG 1 cut(s) 17
BstHHI GCGC 1 cut(s) 19
BstKTI GATC 5 cut(s) 27, 88, 296, 358, 715
BstMAI GTCTC 2 cut(s) 512, 762
BstMBI GATC 5 cut(s) 24, 85, 293, 355, 712
BstMWI GCNNNNNNNGC 3 cut(s) 11, 313, 548
BstNI CCWGG 1 cut(s) 636
BstSCI CCNGG 2 cut(s) 259, 634
BstSFI CTRYAG 1 cut(s) 536
BstUI CGCG 1 cut(s) 17
BstV1I GCAGC 5 cut(s) 160, 379, 522, 707, 731
BstX2I RGATCY 1 cut(s) 293
BstXI CCANNNNNNTGG 1 cut(s) 750
BstYI RGATCY 1 cut(s) 293
BstZ17I GTATAC 1 cut(s) 459
BsuRI GGCC 1 cut(s) 369
BtgI CCRYGG 2 cut(s) 75, 580
BtsCI GGATG 1 cut(s) 654
Cac8I GCNNGC 1 cut(s) 540
CciI TCATGA 1 cut(s) 553
CfoI GCGC 1 cut(s) 19
Csp6I GTAC 2 cut(s) 136, 446
CviAII CATG 3 cut(s) 313, 554, 581
CviQI GTAC 2 cut(s) 136, 446
DdeI CTNAG 4 cut(s) 53, 62, 404, 657
DpnI GATC 5 cut(s) 26, 87, 295, 357, 714
DpnII GATC 5 cut(s) 24, 85, 293, 355, 712
Eam1104I CTCTTC 3 cut(s) 34, 525, 729
EarI CTCTTC 3 cut(s) 34, 525, 729
Eco130I CCWWGG 2 cut(s) 580, 616
EcoNI CCTNNNNNAGG 1 cut(s) 639
EcoRI GAATTC 2 cut(s) 116, 150
EcoRII CCWGG 1 cut(s) 634
EcoT14I CCWWGG 2 cut(s) 580, 616
ErhI CCWWGG 2 cut(s) 580, 616
Esp3I CGTCTC 1 cut(s) 762
FaeI CATG 3 cut(s) 316, 557, 584
FaiI YATR 9 cut(s) 314, 410, 427, 429, 459, 501, 555, 582, 627
FalI AAGNNNNNCTT 4 cut(s) 300, 332, 345, 377
FaqI GGGAC 1 cut(s) 322
FatI CATG 3 cut(s) 312, 553, 580
FblI GTMKAC 1 cut(s) 458
Fnu4HI GCNGC 8 cut(s) 12, 15, 123, 174, 393, 536, 696, 720
FokI GGATG 1 cut(s) 661
Fsp4HI GCNGC 8 cut(s) 12, 15, 123, 174, 393, 536, 696, 720
FspBI CTAG 3 cut(s) 290, 504, 641
GlaI GCGC 1 cut(s) 18
GluI GCNGC 8 cut(s) 12, 15, 123, 174, 393, 536, 696, 720
HaeIII GGCC 1 cut(s) 369
HapII CCGG 2 cut(s) 139, 260
HhaI GCGC 1 cut(s) 19
Hin1II CATG 3 cut(s) 316, 557, 584
Hin6I GCGC 1 cut(s) 17
HinP1I GCGC 1 cut(s) 17
HinfI GANTC 3 cut(s) 256, 283, 400
HpaII CCGG 2 cut(s) 139, 260
Hpy166II GTNNAC 1 cut(s) 459
Hpy188I TCNGA 3 cut(s) 85, 332, 660
Hpy188III TCNNGA 4 cut(s) 130, 320, 419, 554
Hpy8I GTNNAC 1 cut(s) 459
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 1 cut(s) 350
HpyCH4III ACNGT 2 cut(s) 112, 675
HpyCH4IV ACGT 2 cut(s) 335, 596
HpyCH4V TGCA 6 cut(s) 168, 302, 538, 602, 719, 734
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 313, 548
HpyF3I CTNAG 4 cut(s) 53, 62, 404, 657
HpySE526I ACGT 2 cut(s) 335, 596
Hsp92II CATG 3 cut(s) 316, 557, 584
HspAI GCGC 1 cut(s) 17
Kzo9I GATC 5 cut(s) 24, 85, 293, 355, 712
LguI GCTCTTC 2 cut(s) 525, 729
LmnI GCTCC 4 cut(s) 77, 421, 514, 620
LpnPI CCDG 9 cut(s) 152, 174, 273, 351, 432, 456, 524, 621, 648
Lsp1109I GCAGC 5 cut(s) 160, 379, 522, 707, 731
LweI GCATC 2 cut(s) 190, 403
MaeI CTAG 3 cut(s) 290, 504, 641
MaeII ACGT 2 cut(s) 335, 596
MalI GATC 5 cut(s) 26, 87, 295, 357, 714
MboI GATC 5 cut(s) 24, 85, 293, 355, 712
MboII GAAGA 4 cut(s) 21, 542, 680, 716
MfeI CAATTG 1 cut(s) 377
MflI RGATCY 1 cut(s) 293
MluCI AATT 3 cut(s) 116, 150, 377
MmeI TCCRAC 2 cut(s) 127, 645
MnlI CCTC 5 cut(s) 187, 206, 382, 654, 741
MspA1I CMGCKG 1 cut(s) 11
MspI CCGG 2 cut(s) 139, 260
MspR9I CCNGG 2 cut(s) 261, 636
MunI CAATTG 1 cut(s) 377
MvaI CCWGG 1 cut(s) 636
MvnI CGCG 1 cut(s) 17
MwoI GCNNNNNNNGC 3 cut(s) 11, 313, 548
NciI CCSGG 1 cut(s) 261
NcoI CCATGG 1 cut(s) 580
NdeII GATC 5 cut(s) 24, 85, 293, 355, 712
NlaIII CATG 3 cut(s) 316, 557, 584
NlaIV GGNNCC 1 cut(s) 218
NspV TTCGAA 1 cut(s) 350
PagI TCATGA 1 cut(s) 553
PciSI GCTCTTC 2 cut(s) 525, 729
PcsI WCGNNNNNNNCGW 1 cut(s) 688
PfeI GAWTC 3 cut(s) 256, 283, 400
PfoI TCCNGGA 1 cut(s) 259
PkrI GCNGC 8 cut(s) 13, 16, 124, 175, 394, 537, 697, 721
Psp6I CCWGG 1 cut(s) 634
PspGI CCWGG 1 cut(s) 634
PspN4I GGNNCC 1 cut(s) 218
PstI CTGCAG 1 cut(s) 540
PsuI RGATCY 1 cut(s) 293
RsaI GTAC 2 cut(s) 137, 447
RsaNI GTAC 2 cut(s) 136, 446
SapI GCTCTTC 2 cut(s) 525, 729
SatI GCNGC 8 cut(s) 12, 15, 123, 174, 393, 536, 696, 720
Sau3AI GATC 5 cut(s) 24, 85, 293, 355, 712
ScrFI CCNGG 2 cut(s) 261, 636
SfaNI GCATC 2 cut(s) 190, 403
SfcI CTRYAG 1 cut(s) 536
SfuI TTCGAA 1 cut(s) 350
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
SpeI ACTAGT 1 cut(s) 503
Sse9I AATT 3 cut(s) 116, 150, 377
SsiI CCGC 5 cut(s) 9, 15, 94, 123, 395
SspI AATATT 1 cut(s) 607
SspMI CTAG 3 cut(s) 290, 504, 641
StyD4I CCNGG 2 cut(s) 259, 634
StyI CCWWGG 2 cut(s) 580, 616
TaaI ACNGT 2 cut(s) 112, 675
TaiI ACGT 2 cut(s) 338, 599
TaqI TCGA 5 cut(s) 114, 154, 198, 350, 691
TasI AATT 3 cut(s) 116, 150, 377
TauI GCSGC 2 cut(s) 17, 125
TfiI GAWTC 3 cut(s) 256, 283, 400
TseI GCWGC 6 cut(s) 11, 173, 392, 535, 695, 719
TspDTI ATGAA 1 cut(s) 488
TspGWI ACGGA 2 cut(s) 64, 169
XagI CCTNNNNNAGG 1 cut(s) 639
XapI RAATTY 2 cut(s) 116, 150
XmiI GTMKAC 1 cut(s) 458
XspI CTAG 3 cut(s) 290, 504, 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.