Rroxscaffold_2G00077250

Peptidase family M48

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0006709
Physical Location & Seq
Forward (+)
851527 .. 854392
2866 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00077250.1

Sequence Viewer

Length: 948 bp
ATGGCTTCCGTTCCCCTATCTTCTCTTTCCCTCAACCACAACCCTACCTCTCTCAGCTTCACCTCCACAGCTTCCGATCACTTCAGGCTTGCTTCACGTACCTTTCAATTCGCCACCGTCAGCAAATTTCGCTGTGTCAGGTTCGCCAACACGTTGATTTTGAGAGCGATTCCAGGACTAACTGAAGTTGGAAAGGTTCTACTAGGATCTGTTACGGAGCAAGTGATGCTTCTTGAGAATATCGGAACGTCCCTTCTTGTTTCGGAAAATCAGCTTTCTGATCTACATAAATTGATGGTTGAAGCAGCAGACATACTCAACATTGATGCTCCTGATCTATATGTTCGTCAAAGTCCTGTACCAAATGCATACACTTTAGCAATAAGTGGTAAAAAGCCATTTGTTGTTATCCATACTAGTCTAGTGGAGCTTTTGACACGAGAAGAGTTGCAGGCTGTTTTGGCTCATGAGTTGGGTCATTTGAAATGCGACCATGGTGTGTGGCTTACATTTGCAAATATCCTTACCCTTGGAGCTTATACCATACCTGGGCTTGGTGGCATGATTGCTCAGAGTTTAGAAGAGCAGTTATTTCGTTGGCTTCGAGCAGCAGAGCTAACCTGTGATCGTGCAGCCCTTCTTGTTGCTCAAGACCCAAAGGTGGTCGTCTCTGTGTTGATGAAACTAGCTGGTGGATGCCCATCTATGGCTGATCAACTAAATGTGGATGCATTTTTGGAACAAGCTCGCTCTTATGACAAAGCTTCTTCAAGCCCTGTAGGCTGGTATATAAGAAATGCACAAACGAGGCAACTTTCACATCCTTTGCCGGTACTACGAGCTCGTGAAATTGATACATGGTCAAGAAGCCAAGAGTACAGAACTCTTCTAAAACGAGCAACACAGATCACTGCTCGATCAAAAATTTTAACCACACCTGGAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M48

Protein Analysis

315

Amino Acids

34.69

Weight (kDa)

8.9

Isoelectric Point (pI)

47.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M48 PF01435 93 - 287 2.3e-18 Peptidase family M48
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 214
AcsI RAATTY 2 cut(s) 125, 924
AcuI CTGAAG 2 cut(s) 67, 204
AfaI GTAC 4 cut(s) 100, 360, 834, 878
AfiI CCNNNNNNNGG 4 cut(s) 549, 554, 661, 706
AflIII ACRYGT 1 cut(s) 150
AgsI TTSAA 4 cut(s) 107, 302, 484, 771
AhlI ACTAGT 1 cut(s) 416
AjnI CCWGG 3 cut(s) 172, 547, 937
Alw21I GWGCWC 1 cut(s) 844
Alw26I GTCTC 1 cut(s) 673
AlwI GGATC 1 cut(s) 214
ApeKI GCWGC 3 cut(s) 305, 608, 632
ApoI RAATTY 2 cut(s) 125, 924
AsuHPI GGTGA 1 cut(s) 52
BanII GRGCYC 1 cut(s) 844
BauI CACGAG 2 cut(s) 438, 843
Bbv12I GWGCWC 1 cut(s) 844
BbvI GCAGC 3 cut(s) 317, 620, 644
BccI CCATC 2 cut(s) 289, 709
BcgI CGANNNNNNTGC 2 cut(s) 575, 609
BciT130I CCWGG 3 cut(s) 174, 549, 939
BclI TGATCA 1 cut(s) 712
BcoDI GTCTC 1 cut(s) 673
BcuI ACTAGT 1 cut(s) 416
BfaI CTAG 4 cut(s) 203, 417, 422, 686
BfmI CTRYAG 1 cut(s) 777
BglI GCCNNNNNGGC 1 cut(s) 780
BisI GCNGC 3 cut(s) 306, 609, 633
BlsI GCNGC 3 cut(s) 307, 610, 634
Bme1390I CCNGG 3 cut(s) 174, 549, 939
BmrFI CCNGG 3 cut(s) 174, 549, 939
BmsI GCATC 4 cut(s) 216, 316, 686, 718
BpuEI CTTGAG 2 cut(s) 254, 633
BsaAI YACGTR 1 cut(s) 98
BsaBI GATNNNNATC 1 cut(s) 700
BsaJI CCNNGG 3 cut(s) 493, 529, 548
Bsc4I CCNNNNNNNGG 4 cut(s) 549, 554, 661, 706
Bse118I RCCGGY 1 cut(s) 829
Bse8I GATNNNNATC 1 cut(s) 700
BseBI CCWGG 3 cut(s) 174, 549, 939
BseDI CCNNGG 3 cut(s) 493, 529, 548
BseGI GGATG 3 cut(s) 701, 733, 820
BseJI GATNNNNATC 1 cut(s) 700
BseLI CCNNNNNNNGG 4 cut(s) 549, 554, 661, 706
BseMII CTCAG 2 cut(s) 67, 584
BseXI GCAGC 3 cut(s) 317, 620, 644
BsgI GTGCAG 1 cut(s) 651
BsiHKAI GWGCWC 1 cut(s) 844
BsiSI CCGG 1 cut(s) 830
BslFI GGGAC 1 cut(s) 235
BslI CCNNNNNNNGG 4 cut(s) 549, 554, 661, 706
BsmAI GTCTC 1 cut(s) 673
BsmBI CGTCTC 1 cut(s) 673
BsmFI GGGAC 1 cut(s) 235
Bsp1286I GDGCHC 1 cut(s) 844
Bsp143I GATC 8 cut(s) 76, 206, 280, 334, 625, 712, 906, 917
Bsp19I CCATGG 1 cut(s) 493
BspCNI CTCAG 2 cut(s) 66, 583
BspHI TCATGA 1 cut(s) 466
BspPI GGATC 1 cut(s) 214
BspQI GCTCTTC 1 cut(s) 576
BsrFI RCCGGY 1 cut(s) 829
BssAI RCCGGY 1 cut(s) 829
BssECI CCNNGG 3 cut(s) 493, 529, 548
BssMI GATC 8 cut(s) 76, 206, 280, 334, 625, 712, 906, 917
BssSI CACGAG 2 cut(s) 438, 843
BssT1I CCWWGG 2 cut(s) 493, 529
Bst2BI CACGAG 2 cut(s) 438, 843
Bst2UI CCWGG 3 cut(s) 174, 549, 939
Bst4CI ACNGT 1 cut(s) 118
Bst6I CTCTTC 3 cut(s) 438, 576, 891
BstAPI GCANNNNNTGC 1 cut(s) 226
BstBAI YACGTR 1 cut(s) 98
BstC8I GCNNGC 3 cut(s) 90, 453, 748
BstDEI CTNAG 2 cut(s) 53, 570
BstDSI CCRYGG 1 cut(s) 493
BstF5I GGATG 3 cut(s) 701, 733, 820
BstKTI GATC 8 cut(s) 79, 209, 283, 337, 628, 715, 909, 920
BstMAI GTCTC 1 cut(s) 673
BstMBI GATC 8 cut(s) 76, 206, 280, 334, 625, 712, 906, 917
BstMWI GCNNNNNNNGC 4 cut(s) 129, 226, 461, 780
BstNI CCWGG 3 cut(s) 174, 549, 939
BstSCI CCNGG 3 cut(s) 172, 547, 937
BstSFI CTRYAG 1 cut(s) 777
BstV1I GCAGC 3 cut(s) 317, 620, 644
BstX2I RGATCY 1 cut(s) 206
BstYI RGATCY 1 cut(s) 206
BtgI CCRYGG 1 cut(s) 493
BtsCI GGATG 3 cut(s) 701, 733, 820
BtsI GCAGTG 1 cut(s) 909
BtsIMutI CAGTG 1 cut(s) 909
Cac8I GCNNGC 3 cut(s) 90, 453, 748
CciI TCATGA 1 cut(s) 466
Cfr10I RCCGGY 1 cut(s) 829
Csp6I GTAC 4 cut(s) 99, 359, 833, 877
CviAII CATG 4 cut(s) 467, 494, 562, 858
CviQI GTAC 4 cut(s) 99, 359, 833, 877
DdeI CTNAG 2 cut(s) 53, 570
DpnI GATC 8 cut(s) 78, 208, 282, 336, 627, 714, 908, 919
DpnII GATC 8 cut(s) 76, 206, 280, 334, 625, 712, 906, 917
Eam1104I CTCTTC 3 cut(s) 438, 576, 891
EarI CTCTTC 3 cut(s) 438, 576, 891
Ecl136II GAGCTC 1 cut(s) 842
Eco130I CCWWGG 2 cut(s) 493, 529
Eco24I GRGCYC 1 cut(s) 844
Eco53kI GAGCTC 1 cut(s) 842
Eco57I CTGAAG 2 cut(s) 67, 204
EcoICRI GAGCTC 1 cut(s) 842
EcoRII CCWGG 3 cut(s) 172, 547, 937
EcoT14I CCWWGG 2 cut(s) 493, 529
EcoT22I ATGCAT 2 cut(s) 370, 733
EcoT38I GRGCYC 1 cut(s) 844
ErhI CCWWGG 2 cut(s) 493, 529
Esp3I CGTCTC 1 cut(s) 673
FaeI CATG 4 cut(s) 470, 497, 565, 861
FalI AAGNNNNNCTT 2 cut(s) 213, 245
FaqI GGGAC 1 cut(s) 235
FatI CATG 4 cut(s) 466, 493, 561, 857
FbaI TGATCA 1 cut(s) 712
Fnu4HI GCNGC 3 cut(s) 306, 609, 633
FokI GGATG 3 cut(s) 708, 740, 807
FriOI GRGCYC 1 cut(s) 844
Fsp4HI GCNGC 3 cut(s) 306, 609, 633
FspBI CTAG 4 cut(s) 203, 417, 422, 686
GluI GCNGC 3 cut(s) 306, 609, 633
HapII CCGG 1 cut(s) 830
Hin1II CATG 4 cut(s) 470, 497, 565, 861
HindIII AAGCTT 1 cut(s) 762
HinfI GANTC 1 cut(s) 169
HpaII CCGG 1 cut(s) 830
HphI GGTGA 1 cut(s) 52
Hpy188I TCNGA 5 cut(s) 76, 245, 265, 280, 573
Hpy188III TCNNGA 6 cut(s) 233, 332, 467, 650, 845, 864
HpyAV CCTTC 2 cut(s) 263, 647
HpyCH4III ACNGT 1 cut(s) 118
HpyCH4IV ACGT 3 cut(s) 97, 152, 248
HpyCH4V TGCA 6 cut(s) 368, 451, 515, 632, 731, 800
HpyF10VI GCNNNNNNNGC 4 cut(s) 129, 226, 461, 780
HpyF3I CTNAG 2 cut(s) 53, 570
HpySE526I ACGT 3 cut(s) 97, 152, 248
Hsp92II CATG 4 cut(s) 470, 497, 565, 861
Ksp22I TGATCA 1 cut(s) 712
Kzo9I GATC 8 cut(s) 76, 206, 280, 334, 625, 712, 906, 917
LguI GCTCTTC 1 cut(s) 576
LmnI GCTCC 4 cut(s) 217, 334, 427, 533
Lsp1109I GCAGC 3 cut(s) 317, 620, 644
LweI GCATC 4 cut(s) 216, 316, 686, 718
MaeI CTAG 4 cut(s) 203, 417, 422, 686
MaeII ACGT 3 cut(s) 97, 152, 248
MaeIII GTNAC 1 cut(s) 211
MalI GATC 8 cut(s) 78, 208, 282, 336, 627, 714, 908, 919
MboI GATC 8 cut(s) 76, 206, 280, 334, 625, 712, 906, 917
MboII GAAGA 5 cut(s) 12, 455, 593, 759, 878
MflI RGATCY 1 cut(s) 206
MhlI GDGCHC 1 cut(s) 844
MluCI AATT 5 cut(s) 107, 125, 290, 849, 924
MmeI TCCRAC 1 cut(s) 169
MnlI CCTC 4 cut(s) 41, 58, 73, 801
Mph1103I ATGCAT 2 cut(s) 370, 733
MseI TTAA 1 cut(s) 929
MspI CCGG 1 cut(s) 830
MspR9I CCNGG 3 cut(s) 174, 549, 939
MvaI CCWGG 3 cut(s) 174, 549, 939
MwoI GCNNNNNNNGC 4 cut(s) 129, 226, 461, 780
NcoI CCATGG 1 cut(s) 493
NdeII GATC 8 cut(s) 76, 206, 280, 334, 625, 712, 906, 917
NlaIII CATG 4 cut(s) 470, 497, 565, 861
NsiI ATGCAT 2 cut(s) 370, 733
PagI TCATGA 1 cut(s) 466
PciSI GCTCTTC 1 cut(s) 576
PcsI WCGNNNNNNNCGW 1 cut(s) 601
PfeI GAWTC 1 cut(s) 169
PfoI TCCNGGA 1 cut(s) 172
PkrI GCNGC 3 cut(s) 307, 610, 634
Ppu21I YACGTR 1 cut(s) 98
Psp124BI GAGCTC 1 cut(s) 844
Psp6I CCWGG 3 cut(s) 172, 547, 937
PspGI CCWGG 3 cut(s) 172, 547, 937
PsuI RGATCY 1 cut(s) 206
RsaI GTAC 4 cut(s) 100, 360, 834, 878
RsaNI GTAC 4 cut(s) 99, 359, 833, 877
SacI GAGCTC 1 cut(s) 844
SapI GCTCTTC 1 cut(s) 576
SaqAI TTAA 1 cut(s) 929
SatI GCNGC 3 cut(s) 306, 609, 633
Sau3AI GATC 8 cut(s) 76, 206, 280, 334, 625, 712, 906, 917
ScrFI CCNGG 3 cut(s) 174, 549, 939
SduI GDGCHC 1 cut(s) 844
SfaNI GCATC 4 cut(s) 216, 316, 686, 718
SfcI CTRYAG 1 cut(s) 777
SmlI CTYRAG 2 cut(s) 233, 648
SmoI CTYRAG 2 cut(s) 233, 648
SpeI ACTAGT 1 cut(s) 416
Sse9I AATT 5 cut(s) 107, 125, 290, 849, 924
SspMI CTAG 4 cut(s) 203, 417, 422, 686
SstI GAGCTC 1 cut(s) 844
StyD4I CCNGG 3 cut(s) 172, 547, 937
StyI CCWWGG 2 cut(s) 493, 529
TaaI ACNGT 1 cut(s) 118
TaiI ACGT 3 cut(s) 100, 155, 251
TaqI TCGA 2 cut(s) 604, 916
TasI AATT 5 cut(s) 107, 125, 290, 849, 924
TatI WGTACW 1 cut(s) 876
TfiI GAWTC 1 cut(s) 169
Tru1I TTAA 1 cut(s) 929
Tru9I TTAA 1 cut(s) 929
TscAI CASTG 1 cut(s) 916
TseI GCWGC 3 cut(s) 305, 608, 632
TspDTI ATGAA 1 cut(s) 695
TspGWI ACGGA 1 cut(s) 230
TspRI CASTG 1 cut(s) 916
XapI RAATTY 2 cut(s) 125, 924
XspI CTAG 4 cut(s) 203, 417, 422, 686
Zsp2I ATGCAT 2 cut(s) 370, 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.