Rh2AG665000

Peptidase family M48

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0006709
Physical Location & Seq
Reverse (-)
88013577 .. 88016084
2508 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG665000.1

Sequence Viewer

Length: 873 bp
ATGGCGCCAAACGTCTACCATAAGAAGCTAAGAAACGGCTGTGGACACGAACGCGAAACGTACAAACGCTTAACGGCTTTAAACCCAAAATCACACGCCTCTCTACTCTGTAACTCCATTACTATTGCAGGATCTGTTACGGAGCAAGTGATGCTTCTTGAGAATATCGGAACGTCCCTTCTTGTTTCGGAAAATCAGCTTTCTGATCTACATAAATTGATGGTTGAAGCCGCGGACATACTCAACATTGATGCTCCTGATCTATATGTTCGTCAAAGTCCTGTACCAAATGCATACACTTTAGCAATAAGTGGTAAAAAGCCATTTGTTGTTATCCATACTAGTCTAGTGGAGCTTTTGACACGAGAAGAGTTGCAGGCTGTTTTGGCTCATGAGTTGGGTCATTTGAAATGCGACCATGGTGTGTGGCTTACATTTGCAAATATCCTTACCCTTGGAGCTTATACCATACCTGGGCTTGGTGGCATGATTGCTCAGAGTTTAGAAGAGCAGTTATTTCGTTGGCTTCGAGCAGCAGAGCTAACCTGTGATCGTGCAGCCCTTCTTGTTGCTCAAGACCCAAAGGTGGTCGTCTCTGTGTTGATGAAACTAGCTGGTGGATGCCCATCTATGGCTGATCAACTAAATGTGGATGCATTTTTGGAACAAGCTCGCTCTTATGACAAAGCTTCTTCAAGCCCTGTAGGCTGGTATATAAGAAATGCACAAACGAGGCAACTTTCACATCCTTTGCCGGTACTACGAGCTCGTGAAATTGATACATGGTCAAGAAGTCAAGAGTACAGAACTCTTTTAAAACGAGCAACACAGATCACTGCTGGATCAAAAATTTTAACAACACCTGGAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M48

Protein Analysis

290

Amino Acids

32.0

Weight (kDa)

8.55

Isoelectric Point (pI)

49.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M48 PF01435 68 - 262 1.8e-18 Peptidase family M48
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 15
AccII CGCG 2 cut(s) 54, 233
AciI CCGC 2 cut(s) 231, 233
AclWI GGATC 2 cut(s) 139, 850
AcsI RAATTY 1 cut(s) 849
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 62, 285, 759, 803
AfiI CCNNNNNNNGG 4 cut(s) 474, 479, 586, 631
AgsI TTSAA 3 cut(s) 227, 409, 696
AhlI ACTAGT 1 cut(s) 341
AjnI CCWGG 2 cut(s) 472, 862
AluBI AGCT 9 cut(s) 28, 199, 355, 461, 541, 614, 671, 689, 767
AluI AGCT 9 cut(s) 28, 199, 355, 461, 541, 614, 671, 689, 767
Alw21I GWGCWC 1 cut(s) 769
Alw26I GTCTC 1 cut(s) 598
AlwI GGATC 2 cut(s) 139, 850
AlwNI CAGNNNCTG 1 cut(s) 134
ApeKI GCWGC 2 cut(s) 533, 557
ApoI RAATTY 1 cut(s) 849
AspLEI GCGC 1 cut(s) 7
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 769
BauI CACGAG 2 cut(s) 363, 768
Bbv12I GWGCWC 1 cut(s) 769
BbvI GCAGC 2 cut(s) 545, 569
BccI CCATC 2 cut(s) 214, 634
BceAI ACGGC 2 cut(s) 52, 90
BcgI CGANNNNNNTGC 2 cut(s) 500, 534
BciT130I CCWGG 2 cut(s) 474, 864
BclI TGATCA 1 cut(s) 637
BcoDI GTCTC 1 cut(s) 598
BcuI ACTAGT 1 cut(s) 341
BfaI CTAG 3 cut(s) 342, 347, 611
BfmI CTRYAG 1 cut(s) 702
BfoI RGCGCY 1 cut(s) 8
BglI GCCNNNNNGGC 1 cut(s) 705
BisI GCNGC 3 cut(s) 231, 534, 558
BlsI GCNGC 3 cut(s) 232, 535, 559
Bme1390I CCNGG 2 cut(s) 474, 864
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 2 cut(s) 474, 864
BmsI GCATC 4 cut(s) 141, 241, 611, 643
BpuEI CTTGAG 2 cut(s) 179, 558
BsaBI GATNNNNATC 1 cut(s) 625
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 4 cut(s) 231, 418, 454, 473
Bsc4I CCNNNNNNNGG 4 cut(s) 474, 479, 586, 631
Bse118I RCCGGY 1 cut(s) 754
Bse8I GATNNNNATC 1 cut(s) 625
BseBI CCWGG 2 cut(s) 474, 864
BseDI CCNNGG 4 cut(s) 231, 418, 454, 473
BseGI GGATG 3 cut(s) 626, 658, 745
BseJI GATNNNNATC 1 cut(s) 625
BseLI CCNNNNNNNGG 4 cut(s) 474, 479, 586, 631
BseMII CTCAG 1 cut(s) 509
BseXI GCAGC 2 cut(s) 545, 569
BsgI GTGCAG 1 cut(s) 576
Bsh1236I CGCG 2 cut(s) 54, 233
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 1 cut(s) 769
BsiSI CCGG 1 cut(s) 755
BslFI GGGAC 1 cut(s) 160
BslI CCNNNNNNNGG 4 cut(s) 474, 479, 586, 631
BsmAI GTCTC 1 cut(s) 598
BsmBI CGTCTC 1 cut(s) 598
BsmFI GGGAC 1 cut(s) 160
Bsp1286I GDGCHC 1 cut(s) 769
Bsp143I GATC 7 cut(s) 131, 205, 259, 550, 637, 831, 842
Bsp19I CCATGG 1 cut(s) 418
BspACI CCGC 2 cut(s) 231, 233
BspCNI CTCAG 1 cut(s) 508
BspFNI CGCG 2 cut(s) 54, 233
BspHI TCATGA 1 cut(s) 391
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 2 cut(s) 139, 850
BspQI GCTCTTC 1 cut(s) 501
BspT107I GGYRCC 1 cut(s) 4
BsrFI RCCGGY 1 cut(s) 754
BssAI RCCGGY 1 cut(s) 754
BssECI CCNNGG 4 cut(s) 231, 418, 454, 473
BssMI GATC 7 cut(s) 131, 205, 259, 550, 637, 831, 842
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 2 cut(s) 363, 768
BssT1I CCWWGG 2 cut(s) 418, 454
Bst2BI CACGAG 2 cut(s) 363, 768
Bst2UI CCWGG 2 cut(s) 474, 864
Bst6I CTCTTC 2 cut(s) 363, 501
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 151
BstC8I GCNNGC 2 cut(s) 378, 673
BstDEI CTNAG 2 cut(s) 29, 495
BstDSI CCRYGG 2 cut(s) 231, 418
BstF5I GGATG 3 cut(s) 626, 658, 745
BstFNI CGCG 2 cut(s) 54, 233
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 7 cut(s) 134, 208, 262, 553, 640, 834, 845
BstMAI GTCTC 1 cut(s) 598
BstMBI GATC 7 cut(s) 131, 205, 259, 550, 637, 831, 842
BstMWI GCNNNNNNNGC 3 cut(s) 151, 386, 705
BstNI CCWGG 2 cut(s) 474, 864
BstSCI CCNGG 2 cut(s) 472, 862
BstSFI CTRYAG 1 cut(s) 702
BstUI CGCG 2 cut(s) 54, 233
BstV1I GCAGC 2 cut(s) 545, 569
BstX2I RGATCY 1 cut(s) 131
BstYI RGATCY 1 cut(s) 131
BtgI CCRYGG 2 cut(s) 231, 418
BtsCI GGATG 3 cut(s) 626, 658, 745
BtsI GCAGTG 1 cut(s) 834
BtsIMutI CAGTG 1 cut(s) 834
Cac8I GCNNGC 2 cut(s) 378, 673
CaiI CAGNNNCTG 1 cut(s) 134
CciI TCATGA 1 cut(s) 391
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 754
Cfr42I CCGCGG 1 cut(s) 234
Csp6I GTAC 4 cut(s) 61, 284, 758, 802
CviAII CATG 4 cut(s) 392, 419, 487, 783
CviQI GTAC 4 cut(s) 61, 284, 758, 802
DdeI CTNAG 2 cut(s) 29, 495
DinI GGCGCC 1 cut(s) 6
DpnI GATC 7 cut(s) 133, 207, 261, 552, 639, 833, 844
DpnII GATC 7 cut(s) 131, 205, 259, 550, 637, 831, 842
DraI TTTAAA 2 cut(s) 81, 816
Eam1104I CTCTTC 2 cut(s) 363, 501
EarI CTCTTC 2 cut(s) 363, 501
Ecl136II GAGCTC 1 cut(s) 767
Eco130I CCWWGG 2 cut(s) 418, 454
Eco24I GRGCYC 1 cut(s) 769
Eco53kI GAGCTC 1 cut(s) 767
EcoICRI GAGCTC 1 cut(s) 767
EcoRII CCWGG 2 cut(s) 472, 862
EcoT14I CCWWGG 2 cut(s) 418, 454
EcoT22I ATGCAT 2 cut(s) 295, 658
EcoT38I GRGCYC 1 cut(s) 769
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 418, 454
Esp3I CGTCTC 1 cut(s) 598
FaeI CATG 4 cut(s) 395, 422, 490, 786
FalI AAGNNNNNCTT 2 cut(s) 138, 170
FaqI GGGAC 1 cut(s) 160
FatI CATG 4 cut(s) 391, 418, 486, 782
FbaI TGATCA 1 cut(s) 637
FblI GTMKAC 1 cut(s) 15
Fnu4HI GCNGC 3 cut(s) 231, 534, 558
FokI GGATG 3 cut(s) 633, 665, 732
FriOI GRGCYC 1 cut(s) 769
Fsp4HI GCNGC 3 cut(s) 231, 534, 558
FspBI CTAG 3 cut(s) 342, 347, 611
GlaI GCGC 1 cut(s) 6
GluI GCNGC 3 cut(s) 231, 534, 558
HaeII RGCGCY 1 cut(s) 8
HapII CCGG 1 cut(s) 755
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 4 cut(s) 395, 422, 490, 786
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HindIII AAGCTT 1 cut(s) 687
HpaII CCGG 1 cut(s) 755
Hpy166II GTNNAC 2 cut(s) 16, 44
Hpy188I TCNGA 4 cut(s) 170, 190, 205, 498
Hpy188III TCNNGA 7 cut(s) 158, 257, 392, 575, 770, 789, 797
Hpy8I GTNNAC 2 cut(s) 16, 44
HpyAV CCTTC 2 cut(s) 188, 572
HpyCH4IV ACGT 3 cut(s) 12, 59, 173
HpyCH4V TGCA 7 cut(s) 128, 293, 376, 440, 557, 656, 725
HpyF10VI GCNNNNNNNGC 3 cut(s) 151, 386, 705
HpyF3I CTNAG 2 cut(s) 29, 495
HpySE526I ACGT 3 cut(s) 12, 59, 173
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 4 cut(s) 395, 422, 490, 786
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
Ksp22I TGATCA 1 cut(s) 637
KspI CCGCGG 1 cut(s) 234
Kzo9I GATC 7 cut(s) 131, 205, 259, 550, 637, 831, 842
LguI GCTCTTC 1 cut(s) 501
LmnI GCTCC 4 cut(s) 142, 259, 352, 458
Lsp1109I GCAGC 2 cut(s) 545, 569
LweI GCATC 4 cut(s) 141, 241, 611, 643
MaeI CTAG 3 cut(s) 342, 347, 611
MaeII ACGT 3 cut(s) 12, 59, 173
MaeIII GTNAC 2 cut(s) 110, 136
MalI GATC 7 cut(s) 133, 207, 261, 552, 639, 833, 844
MboI GATC 7 cut(s) 131, 205, 259, 550, 637, 831, 842
MboII GAAGA 3 cut(s) 380, 518, 684
MflI RGATCY 1 cut(s) 131
MhlI GDGCHC 1 cut(s) 769
MluCI AATT 3 cut(s) 215, 774, 849
Mly113I GGCGCC 1 cut(s) 5
MnlI CCTC 2 cut(s) 109, 726
Mph1103I ATGCAT 2 cut(s) 295, 658
MseI TTAA 4 cut(s) 71, 80, 815, 854
MspA1I CMGCKG 1 cut(s) 233
MspI CCGG 1 cut(s) 755
MspR9I CCNGG 2 cut(s) 474, 864
MvaI CCWGG 2 cut(s) 474, 864
MvnI CGCG 2 cut(s) 54, 233
MwoI GCNNNNNNNGC 3 cut(s) 151, 386, 705
NarI GGCGCC 1 cut(s) 5
NcoI CCATGG 1 cut(s) 418
NdeII GATC 7 cut(s) 131, 205, 259, 550, 637, 831, 842
NlaIII CATG 4 cut(s) 395, 422, 490, 786
NlaIV GGNNCC 1 cut(s) 6
NsiI ATGCAT 2 cut(s) 295, 658
PagI TCATGA 1 cut(s) 391
PciSI GCTCTTC 1 cut(s) 501
PcsI WCGNNNNNNNCGW 1 cut(s) 526
PkrI GCNGC 3 cut(s) 232, 535, 559
PluTI GGCGCC 1 cut(s) 8
Psp124BI GAGCTC 1 cut(s) 769
Psp6I CCWGG 2 cut(s) 472, 862
PspGI CCWGG 2 cut(s) 472, 862
PspN4I GGNNCC 1 cut(s) 6
PstNI CAGNNNCTG 1 cut(s) 134
PsuI RGATCY 1 cut(s) 131
RsaI GTAC 4 cut(s) 62, 285, 759, 803
RsaNI GTAC 4 cut(s) 61, 284, 758, 802
SacI GAGCTC 1 cut(s) 769
SacII CCGCGG 1 cut(s) 234
SapI GCTCTTC 1 cut(s) 501
SaqAI TTAA 4 cut(s) 71, 80, 815, 854
SatI GCNGC 3 cut(s) 231, 534, 558
Sau3AI GATC 7 cut(s) 131, 205, 259, 550, 637, 831, 842
ScrFI CCNGG 2 cut(s) 474, 864
SduI GDGCHC 1 cut(s) 769
SfaNI GCATC 4 cut(s) 141, 241, 611, 643
SfcI CTRYAG 1 cut(s) 702
SfoI GGCGCC 1 cut(s) 6
Sfr303I CCGCGG 1 cut(s) 234
SgrBI CCGCGG 1 cut(s) 234
SmlI CTYRAG 2 cut(s) 158, 573
SmoI CTYRAG 2 cut(s) 158, 573
SpeI ACTAGT 1 cut(s) 341
Sse9I AATT 3 cut(s) 215, 774, 849
SsiI CCGC 2 cut(s) 231, 233
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 3 cut(s) 342, 347, 611
SstI GAGCTC 1 cut(s) 769
StyD4I CCNGG 2 cut(s) 472, 862
StyI CCWWGG 2 cut(s) 418, 454
TaiI ACGT 3 cut(s) 15, 62, 176
TaqI TCGA 1 cut(s) 529
TasI AATT 3 cut(s) 215, 774, 849
TatI WGTACW 1 cut(s) 801
TauI GCSGC 1 cut(s) 233
Tru1I TTAA 4 cut(s) 71, 80, 815, 854
Tru9I TTAA 4 cut(s) 71, 80, 815, 854
TscAI CASTG 1 cut(s) 841
TseI GCWGC 2 cut(s) 533, 557
TspDTI ATGAA 1 cut(s) 620
TspGWI ACGGA 1 cut(s) 155
TspRI CASTG 1 cut(s) 841
XapI RAATTY 1 cut(s) 849
XmiI GTMKAC 1 cut(s) 15
XspI CTAG 3 cut(s) 342, 347, 611
Zsp2I ATGCAT 2 cut(s) 295, 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.