Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0003908

Physical Location & Seq

Genomic Coordinates Forward (+)

3316046 .. 3319861

Gene Structure 3816 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1049800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2202 bp

ATGAGTGCTCAGAAGAAGAGGAACTTCCAAATTGAGGCCTTCAAGCATCGGGTCGTGGTTGATCCCAAGTACGCTGAGAAGACGTGGAAGATCCTGGAGCATGCGATTCATGAAATTTACAATCACAACGCTAGCGGGCTTAGCTTCGAGGAGCTCTACAGGAATGCTTACAATATGGTGCTGCACAAATTTGGAGAGAAACTCTACTCTGGTCTGGTGACAACCATGACTTATCATCTAAAAGAAATATCTAAGTCAATTGAAGCTGCGCACGGTGAGTTGTTTTTGGAAGAGCTGAACCGGAAGTGGGCAGAGCATAACAAAGCGTTGCAGATGATTCGAGACATATTGATGTACATGGACAGGACTTTCATCCCGAGCACCCACAAAACCCCTGTTCATGAGCTTGGGTTAAACCTGTGGAGGGATGTTGTTATCCACGCTACCAAAACCCAGTCAAGGCTGTTGGATACACTTCTTGAGCTTGTGCATAGAGAAAGGAGTGGTGAAGTAATTAACAGAGGCTTGATGAGGAATATTATAAAAATGTTAATGGATTTAGGTTCTACTGTTTACCAAGACGACTTTGAGAAGCATTTTCTTGAGGTTTCAGCTGATTTTTACCGCTGTGAGTCTCAGGAGTTCATAGAGTCATGTGATTGTGGGAACTATTTAAAGAAGGCTGAGAGACGCTTAATGGAAGAAATGGAGAGAGTCTCCCAGTATTTGGATGCTAGAAGTGAAGCTAAGATAACTAATGTGGTGGAGAAGGAGATGATTGAAAGTCACATGAACAGACTAGTTCATATGGAGAGCTCAGGTTTAGTTAATATGCTTGTGGATGACAAATATGACGACTTGGGAAGAATGTATAGCTTGTTTCGCAGGGTTCAGAATGGACTTGTAATAGTACGAGATGTCATGACCGCTTACATCCGGGATACTGGTAAGCAGCTAGTTACTGACCCAGAAAGGTTAAGGGATCCTGTAGATTTTGTGCAGCGTCTCCTGGATTTGAAGGATAAATATGACAAAGTTATCAGCTCAGCGTTTAACAATGACAAAACATTCCAAAATGCTCTAAATTCCTCTTTTGAATACTTCATTAATTTGAATGCCCGGTCCCCAGAGTTCATATCTCTGTTTGTGGATGACAAGCTTCGGAAAGGACTAAGAGGGGTTAGTGAGGAGGATGTAGAGGTTGTACTGGACAAGGTCATGATGCTTTTCCGTTATCTTCATGAGAAAGATGTCTTTGAAAAGTATTACAAGCAACATTTGGCAAAGAGGCTTCTTTCTGGGAAGACTGTTTCTGATGATGCGGAAAGAAGTCTGATTGTTAAGCTCAAAACAGAGTGCGGATATCAGTTTACTTCTAAATTGGAGGGTATGTTCACTGATATGAAGACCTCTCAGGATACAATGCATGGATTCTACTCAACTGTCGGAGCTCTGTTAGGCGACAGCCCAACGCTAACTGTCCAGGTCCTCACCACAGGTTCATGGCCTACTCAACCAAGTGCCAGCTGCAACCTTCCAGCAGAAATTGTGTGGGTATGTGAGAAGTTCAAGAGTTACTATCTTGGGACCCATACTGGTCGGAGATTGTCCTGGCAAACTAATATGGGCACAGCTGATTTGAAATCAACCTTTGGAAAGGGCCAGAAGCATGAGCTGAATGTTTCCACATATCAGATGTGTGTACTGATGCTATTCAACGACGCTGACCGGTTGACGTATAAGAAAATTGAGCAGGCTACAGAGATACCAGCCGTTGACTTGAAGAGGTGCCTGCAATCTCTGGCCTGTGTTAAGGGGAAGAATGTTCTTCGGAAGGAGCCAATGAGCAAGGACATAGTTGAGGAGGATGTCTTCTTCTTTAATGACAAGTTCACAAGCAAGTACTTCAAGGTAAAGATAGGTACTGTGGTTGCACAAAGGGAGTCTGAACCAGAAAATCAAGAAACCCGACAGAGAGTAGAGGAGGACAGGAAGCCCCAGATTGAAGCAGCAATTGTGAGGATCATGAAGTCAAGGCGGGTCCTGGATCACAATAACATTGTCGCGGAGGTGACGAAACAGCTGCAGGCGCGATTCTTGCCCAACCCTGTTGTAATAAAGAAACGAATCGAGTCCCTCATTGAGCGGGAGTTTTTGGAGAGGGATAAAACGGATCGAAAATTGTACAGGTACCTTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04120 ko04340 ko04341 map04120 map04340 map04341

Pfam Domains

Protein Families

Cullin Cullin_Nedd8

Protein Analysis

734

Amino Acids

85.55

Weight (kDa)

8.29

Isoelectric Point (pI)

42.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cullin	PF00888	29 - 475	3.1e-163	Cullin alpha solenoid domain
Cullin_AB	PF26557	496 - 631	5.4e-50	Cullin alpha+beta domain
Cullin_Nedd8	PF10557	663 - 725	3e-26	Cullin protein neddylation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003908)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G26830 AT1G69670 AT1G69670
fragaria_vesca	FvH4_4g31500 FvH4_6g48910
malus_domestica	MD09G1049800.v1.1 MD16G1074600.v1.1 MD17G1050100.v1.1
prunus_persica	Prupe.1G272800_v2.0.a1 Prupe.3G269000_v2.0.a1
pyrus_communis	pycom111g04080 pycom16g06460 pycom17g04490 pycom17g04500
rosa_chinensis	RchiOBHm_Chr2g0168721 RchiOBHm_Chr4g0439991
rosa_laevigata	RLG00000006217 RLG00000021804
rosa_multiflora	Rmu_sc0006000.1_g000007 Rmu_sc0034426.1_g000001
rosa_roxburghii	Rroxscaffold_2G00082740 Rroxscaffold_5G00380940
rosa_rugosa	Rorug02G0535000 Rorug04G0323000
rosa_samantha	Rh2AG605700 Rh2BG618700 Rh2CG586800 Rh2DG629200 Rh4AG374100 Rh4BG386400 Rh4CG400900 Rh4DG380700
rosa_wichuraiana	Rw2G050210 Rw4G032000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	542
Acc16I	TGCGCA	1 cut(s)	270
Acc65I	GGTACC	1 cut(s)	2190
AccB1I	GGYRCC	2 cut(s)	1788, 2190
AccB7I	CCANNNNNTGG	1 cut(s)	727
AccBSI	CCGCTC	1 cut(s)	2146
AccII	CGCG	2 cut(s)	2066, 2092
AciI	CCGC	8 cut(s)	135, 625, 927, 1322, 1359, 2038, 2066, 2146
AclWI	GGATC	7 cut(s)	56, 85, 977, 990, 2030, 2055, 2181
AcsI	RAATTY	3 cut(s)	114, 188, 1084
AfaI	GTAC	9 cut(s)	71, 356, 912, 1206, 1704, 1904, 1924, 2186, 2192
AfiI	CCNNNNNNNGG	5 cut(s)	34, 307, 424, 459, 727
AgeI	ACCGGT	1 cut(s)	1728
AhlI	ACTAGT	1 cut(s)	799
AjiI	CACGTC	1 cut(s)	84
AjnI	CCWGG	5 cut(s)	93, 1008, 1482, 1610, 2043
AloI	GAACNNNNNNTCC	2 cut(s)	1376, 1408
Alw21I	GWGCWC	5 cut(s)	10, 156, 383, 818, 1453
Alw26I	GTCTC	5 cut(s)	336, 639, 682, 721, 1010
AlwI	GGATC	7 cut(s)	56, 85, 977, 990, 2030, 2055, 2181
Ama87I	CYCGRG	1 cut(s)	376
AoxI	GGCC	4 cut(s)	36, 1505, 1660, 1803
ApeKI	GCWGC	7 cut(s)	181, 266, 952, 1000, 1527, 2009, 2083
ApoI	RAATTY	3 cut(s)	114, 188, 1084
AseI	ATTAAT	1 cut(s)	1107
AsiGI	ACCGGT	1 cut(s)	1728
Asp718I	GGTACC	1 cut(s)	2190
AspLEI	GCGC	2 cut(s)	271, 2092
AspS9I	GGNCC	5 cut(s)	1122, 1486, 1587, 1660, 2041
AsuC2I	CCSGG	2 cut(s)	938, 1120
AsuHPI	GGTGA	5 cut(s)	229, 287, 518, 1483, 2083
AsuNHI	GCTAGC	1 cut(s)	131
AvaI	CYCGRG	1 cut(s)	376
AvaII	GGWCC	4 cut(s)	1122, 1486, 1587, 2041
BaeGI	GKGCMC	1 cut(s)	1631
BaeI	ACNNNNGTAYC	2 cut(s)	1914, 1947
BamHI	GGATCC	1 cut(s)	982
BanI	GGYRCC	2 cut(s)	1788, 2190
BanII	GRGCYC	3 cut(s)	156, 818, 1453
BbsI	GAAGAC	4 cut(s)	86, 1310, 1412, 1864
Bbv12I	GWGCWC	5 cut(s)	10, 156, 383, 818, 1453
BbvI	GCAGC	7 cut(s)	168, 253, 964, 1012, 1514, 2021, 2070
BceAI	ACGGC	1 cut(s)	1757
BcgI	CGANNNNNNTGC	4 cut(s)	320, 354, 2065, 2099
BciT130I	CCWGG	5 cut(s)	95, 1010, 1484, 1612, 2045
BciVI	GTATCC	3 cut(s)	463, 934, 1411
BcnI	CCSGG	2 cut(s)	938, 1120
BcoDI	GTCTC	5 cut(s)	336, 639, 682, 721, 1010
BcuI	ACTAGT	1 cut(s)	799
BfaI	CTAG	4 cut(s)	132, 735, 800, 956
BfmI	CTRYAG	4 cut(s)	157, 987, 1758, 2084
BfuI	GTATCC	3 cut(s)	463, 934, 1411
BisI	GCNGC	7 cut(s)	182, 267, 953, 1001, 1528, 2010, 2084
BlpI	GCTNAGC	2 cut(s)	140, 1045
BlsI	GCNGC	7 cut(s)	183, 268, 954, 1002, 1529, 2011, 2085
BmcAI	AGTACT	1 cut(s)	1904
Bme1390I	CCNGG	7 cut(s)	95, 938, 1010, 1120, 1484, 1612, 2045
Bme18I	GGWCC	4 cut(s)	1122, 1486, 1587, 2041
BmeT110I	CYCGRG	1 cut(s)	376
BmgBI	CACGTC	1 cut(s)	84
BmgT120I	GGNCC	5 cut(s)	1122, 1486, 1587, 1660, 2041
BmiI	GGNNCC	8 cut(s)	984, 1124, 1588, 1589, 1790, 1839, 2042, 2192
BmrFI	CCNGG	7 cut(s)	95, 938, 1010, 1120, 1484, 1612, 2045
BmrI	ACTGGG	2 cut(s)	448, 715
BmsI	GCATC	5 cut(s)	55, 721, 1212, 1309, 1698
BmtI	GCTAGC	1 cut(s)	135
BmuI	ACTGGG	2 cut(s)	448, 715
BpiI	GAAGAC	4 cut(s)	86, 1310, 1412, 1864
BplI	GAGNNNNNCTC	4 cut(s)	186, 218, 701, 733
BpmI	CTGGAG	1 cut(s)	116
Bpu10I	CCTNAGC	1 cut(s)	817
Bpu1102I	GCTNAGC	2 cut(s)	140, 1045
BpuEI	CTTGAG	2 cut(s)	500, 623
BpuMI	CCSGG	2 cut(s)	938, 1120
BsaWI	WCCGGW	2 cut(s)	300, 1728
BsaXI	ACNNNNNCTCC	2 cut(s)	1376, 1406
Bsc4I	CCNNNNNNNGG	5 cut(s)	34, 307, 424, 459, 727
Bse118I	RCCGGY	1 cut(s)	1728
Bse1I	ACTGG	5 cut(s)	454, 721, 949, 1212, 1600
BseBI	CCWGG	5 cut(s)	95, 1010, 1484, 1612, 2045
BseGI	GGATG	8 cut(s)	372, 433, 736, 847, 933, 1156, 1198, 1873
BseLI	CCNNNNNNNGG	5 cut(s)	34, 307, 424, 459, 727
BseMII	CTCAG	7 cut(s)	23, 66, 650, 675, 831, 1059, 1427
BseNI	ACTGG	5 cut(s)	454, 721, 949, 1212, 1600
BseRI	GAGGAG	4 cut(s)	164, 1202, 1877, 1997
BseSI	GKGCMC	1 cut(s)	1631
BseXI	GCAGC	7 cut(s)	168, 253, 964, 1012, 1514, 2021, 2070
BsgI	GTGCAG	2 cut(s)	167, 1019
Bsh1236I	CGCG	2 cut(s)	2066, 2092
BshFI	GGCC	4 cut(s)	38, 1507, 1662, 1805
BshNI	GGYRCC	2 cut(s)	1788, 2190
BshTI	ACCGGT	1 cut(s)	1728
BsiHKAI	GWGCWC	5 cut(s)	10, 156, 383, 818, 1453
BsiHKCI	CYCGRG	1 cut(s)	376
BsiSI	CCGG	4 cut(s)	301, 937, 1120, 1729
BslFI	GGGAC	3 cut(s)	1108, 1600, 2119
BslI	CCNNNNNNNGG	5 cut(s)	34, 307, 424, 459, 727
BsmAI	GTCTC	5 cut(s)	336, 639, 682, 721, 1010
BsmBI	CGTCTC	2 cut(s)	682, 1010
BsmFI	GGGAC	3 cut(s)	1108, 1600, 2119
BsmI	GAATGC	2 cut(s)	169, 1120
BsnI	GGCC	4 cut(s)	38, 1507, 1662, 1805
BsoBI	CYCGRG	1 cut(s)	376
Bsp1286I	GDGCHC	6 cut(s)	10, 156, 383, 818, 1453, 1631
Bsp1407I	TGTACA	2 cut(s)	354, 2184
Bsp143I	GATC	6 cut(s)	61, 90, 982, 2022, 2047, 2173
Bsp1720I	GCTNAGC	2 cut(s)	140, 1045
BspACI	CCGC	8 cut(s)	135, 625, 927, 1322, 1359, 2038, 2066, 2146
BspANI	GGCC	4 cut(s)	38, 1507, 1662, 1805
BspCNI	CTCAG	7 cut(s)	22, 67, 649, 676, 830, 1058, 1426
BspFNI	CGCG	2 cut(s)	2066, 2092
BspHI	TCATGA	6 cut(s)	109, 400, 921, 1218, 1240, 2025
BspLI	GGNNCC	8 cut(s)	984, 1124, 1588, 1589, 1790, 1839, 2042, 2192
BspMAI	CTGCAG	1 cut(s)	2088
BspOI	GCTAGC	1 cut(s)	135
BspPI	GGATC	7 cut(s)	56, 85, 977, 990, 2030, 2055, 2181
BspQI	GCTCTTC	1 cut(s)	285
BspT107I	GGYRCC	2 cut(s)	1788, 2190
BsrBI	CCGCTC	1 cut(s)	2146
BsrFI	RCCGGY	1 cut(s)	1728
BsrGI	TGTACA	2 cut(s)	354, 2184
BsrI	ACTGG	5 cut(s)	454, 721, 949, 1212, 1600
BssAI	RCCGGY	1 cut(s)	1728
BssMI	GATC	6 cut(s)	61, 90, 982, 2022, 2047, 2173
Bst2UI	CCWGG	5 cut(s)	95, 1010, 1484, 1612, 2045
Bst4CI	ACNGT	6 cut(s)	275, 571, 1309, 1444, 1480, 1927
Bst6I	CTCTTC	3 cut(s)	11, 285, 1778
BstAUI	TGTACA	2 cut(s)	354, 2184
BstC8I	GCNNGC	7 cut(s)	102, 133, 137, 1525, 1755, 1793, 2088
BstF5I	GGATG	8 cut(s)	372, 433, 736, 847, 933, 1156, 1198, 1873
BstFNI	CGCG	2 cut(s)	2066, 2092
BstHHI	GCGC	2 cut(s)	271, 2092
BstKTI	GATC	6 cut(s)	64, 93, 985, 2025, 2050, 2176
BstMAI	GTCTC	5 cut(s)	336, 639, 682, 721, 1010
BstMBI	GATC	6 cut(s)	61, 90, 982, 2022, 2047, 2173
BstMWI	GCNNNNNNNGC	4 cut(s)	141, 882, 2089, 2098
BstNI	CCWGG	5 cut(s)	95, 1010, 1484, 1612, 2045
BstNSI	RCATGY	1 cut(s)	104
BstSCI	CCNGG	7 cut(s)	93, 936, 1008, 1118, 1482, 1610, 2043
BstSFI	CTRYAG	4 cut(s)	157, 987, 1758, 2084
BstSLI	GKGCMC	1 cut(s)	1631
BstUI	CGCG	2 cut(s)	2066, 2092
BstV1I	GCAGC	7 cut(s)	168, 253, 964, 1012, 1514, 2021, 2070
BstV2I	GAAGAC	4 cut(s)	86, 1310, 1412, 1864
BstX2I	RGATCY	2 cut(s)	90, 982
BstYI	RGATCY	2 cut(s)	90, 982
BsuI	GTATCC	3 cut(s)	463, 934, 1411
BsuRI	GGCC	4 cut(s)	38, 1507, 1662, 1805
BtrI	CACGTC	1 cut(s)	84
BtsCI	GGATG	8 cut(s)	372, 433, 736, 847, 933, 1156, 1198, 1873
BtsIMutI	CAGTG	1 cut(s)	1395
Cac8I	GCNNGC	7 cut(s)	102, 133, 137, 1525, 1755, 1793, 2088
CciI	TCATGA	6 cut(s)	109, 400, 921, 1218, 1240, 2025
CfoI	GCGC	2 cut(s)	271, 2092
Cfr10I	RCCGGY	1 cut(s)	1728
Cfr13I	GGNCC	5 cut(s)	1122, 1486, 1587, 1660, 2041
CseI	GACGC	3 cut(s)	699, 992, 1730
Csp6I	GTAC	9 cut(s)	70, 355, 911, 1205, 1703, 1903, 1923, 2185, 2191
CspAI	ACCGGT	1 cut(s)	1728
CviQI	GTAC	9 cut(s)	70, 355, 911, 1205, 1703, 1903, 1923, 2185, 2191
DpnI	GATC	6 cut(s)	63, 92, 984, 2024, 2049, 2175
DpnII	GATC	6 cut(s)	61, 90, 982, 2022, 2047, 2173
DraI	TTTAAA	1 cut(s)	675
Eam1104I	CTCTTC	3 cut(s)	11, 285, 1778
EarI	CTCTTC	3 cut(s)	11, 285, 1778
Ecl136II	GAGCTC	3 cut(s)	154, 816, 1451
Eco147I	AGGCCT	1 cut(s)	38
Eco24I	GRGCYC	3 cut(s)	156, 818, 1453
Eco32I	GATATC	1 cut(s)	1364
Eco47I	GGWCC	4 cut(s)	1122, 1486, 1587, 2041
Eco53kI	GAGCTC	3 cut(s)	154, 816, 1451
Eco88I	CYCGRG	1 cut(s)	376
EcoICRI	GAGCTC	3 cut(s)	154, 816, 1451
EcoO109I	RGGNCCY	3 cut(s)	1486, 1587, 2041
EcoRII	CCWGG	5 cut(s)	93, 1008, 1482, 1610, 2043
EcoRV	GATATC	1 cut(s)	1364
EcoT22I	ATGCAT	1 cut(s)	1428
EcoT38I	GRGCYC	3 cut(s)	156, 818, 1453
Esp3I	CGTCTC	2 cut(s)	682, 1010
FalI	AAGNNNNNCTT	2 cut(s)	8, 40
FaqI	GGGAC	3 cut(s)	1108, 1600, 2119
FauI	CCCGC	3 cut(s)	128, 2031, 2139
FauNDI	CATATG	1 cut(s)	807
Fnu4HI	GCNGC	7 cut(s)	182, 267, 953, 1001, 1528, 2010, 2084
FokI	GGATG	8 cut(s)	359, 440, 743, 854, 920, 1163, 1205, 1880
FriOI	GRGCYC	3 cut(s)	156, 818, 1453
Fsp4HI	GCNGC	7 cut(s)	182, 267, 953, 1001, 1528, 2010, 2084
FspBI	CTAG	4 cut(s)	132, 735, 800, 956
FspI	TGCGCA	1 cut(s)	270
GlaI	GCGC	2 cut(s)	270, 2091
GluI	GCNGC	7 cut(s)	182, 267, 953, 1001, 1528, 2010, 2084
GsuI	CTGGAG	1 cut(s)	116
HaeIII	GGCC	4 cut(s)	38, 1507, 1662, 1805
HapII	CCGG	4 cut(s)	301, 937, 1120, 1729
HgaI	GACGC	3 cut(s)	699, 992, 1730
HhaI	GCGC	2 cut(s)	271, 2092
Hin6I	GCGC	2 cut(s)	269, 2090
HinP1I	GCGC	2 cut(s)	269, 2090
HincII	GTYRAC	2 cut(s)	1734, 1777
HindII	GTYRAC	2 cut(s)	1734, 1777
HindIII	AAGCTT	1 cut(s)	1157
HpaII	CCGG	4 cut(s)	301, 937, 1120, 1729
HphI	GGTGA	5 cut(s)	229, 287, 518, 1483, 2083
Hpy166II	GTNNAC	7 cut(s)	574, 1371, 1395, 1703, 1734, 1777, 1893
Hpy8I	GTNNAC	7 cut(s)	574, 1371, 1395, 1703, 1734, 1777, 1893
Hpy99I	CGWCG	1 cut(s)	1724
HpyAV	CCTTC	6 cut(s)	49, 673, 763, 1012, 1544, 1828
HpyCH4III	ACNGT	6 cut(s)	275, 571, 1309, 1444, 1480, 1927
HpyCH4IV	ACGT	2 cut(s)	83, 1736
HpyCH4V	TGCA	9 cut(s)	184, 331, 490, 1000, 1426, 1530, 1795, 1934, 2086
HpyF10VI	GCNNNNNNNGC	4 cut(s)	141, 882, 2089, 2098
HpySE526I	ACGT	2 cut(s)	83, 1736
HspAI	GCGC	2 cut(s)	269, 2090
KflI	GGGWCCC	1 cut(s)	1587
KpnI	GGTACC	1 cut(s)	2194
Kzo9I	GATC	6 cut(s)	61, 90, 982, 2022, 2047, 2173
LguI	GCTCTTC	1 cut(s)	285
LmnI	GCTCC	4 cut(s)	97, 151, 1448, 1837
Lsp1109I	GCAGC	7 cut(s)	168, 253, 964, 1012, 1514, 2021, 2070
LweI	GCATC	5 cut(s)	55, 721, 1212, 1309, 1698
MaeI	CTAG	4 cut(s)	132, 735, 800, 956
MaeII	ACGT	2 cut(s)	83, 1736
MaeIII	GTNAC	5 cut(s)	217, 785, 958, 1574, 2071
MalI	GATC	6 cut(s)	63, 92, 984, 2024, 2049, 2175
MbiI	CCGCTC	1 cut(s)	2146
MboI	GATC	6 cut(s)	61, 90, 982, 2022, 2047, 2173
MfeI	CAATTG	2 cut(s)	258, 2013
MflI	RGATCY	2 cut(s)	90, 982
MhlI	GDGCHC	6 cut(s)	10, 156, 383, 818, 1453, 1631
MlyI	GAGTC	5 cut(s)	641, 659, 723, 1952, 2141
MmeI	TCCRAC	3 cut(s)	447, 1426, 1580
Mph1103I	ATGCAT	1 cut(s)	1428
MslI	CAYNNNNRTG	2 cut(s)	350, 1400
MspA1I	CMGCKG	5 cut(s)	614, 627, 1527, 1634, 2083
MspI	CCGG	4 cut(s)	301, 937, 1120, 1729
MspR9I	CCNGG	7 cut(s)	95, 938, 1010, 1120, 1484, 1612, 2045
MunI	CAATTG	2 cut(s)	258, 2013
Mva1269I	GAATGC	2 cut(s)	169, 1120
MvaI	CCWGG	5 cut(s)	95, 1010, 1484, 1612, 2045
MvnI	CGCG	2 cut(s)	2066, 2092
MwoI	GCNNNNNNNGC	4 cut(s)	141, 882, 2089, 2098
NciI	CCSGG	2 cut(s)	938, 1120
NdeI	CATATG	1 cut(s)	807
NdeII	GATC	6 cut(s)	61, 90, 982, 2022, 2047, 2173
NheI	GCTAGC	1 cut(s)	131
NlaIV	GGNNCC	8 cut(s)	984, 1124, 1588, 1589, 1790, 1839, 2042, 2192
NmuCI	GTSAC	3 cut(s)	217, 785, 2071
NsbI	TGCGCA	1 cut(s)	270
NsiI	ATGCAT	1 cut(s)	1428
NspI	RCATGY	1 cut(s)	104
PaeI	GCATGC	1 cut(s)	104
PagI	TCATGA	6 cut(s)	109, 400, 921, 1218, 1240, 2025
PceI	AGGCCT	1 cut(s)	38
PciSI	GCTCTTC	1 cut(s)	285
PctI	GAATGC	2 cut(s)	169, 1120
PfeI	GAWTC	5 cut(s)	106, 337, 1431, 2094, 2127
PflFI	GACNNNGTC	1 cut(s)	1214
PflMI	CCANNNNNTGG	1 cut(s)	727
PfoI	TCCNGGA	4 cut(s)	93, 936, 1008, 2043
PinAI	ACCGGT	1 cut(s)	1728
PkrI	GCNGC	7 cut(s)	183, 268, 954, 1002, 1529, 2011, 2085
PleI	GAGTC	5 cut(s)	640, 658, 722, 1951, 2140
PpsI	GAGTC	5 cut(s)	640, 658, 722, 1951, 2140
PpuMI	RGGWCCY	3 cut(s)	1486, 1587, 2041
PshBI	ATTAAT	1 cut(s)	1107
PsiI	TTATAA	1 cut(s)	542
Psp124BI	GAGCTC	3 cut(s)	156, 818, 1453
Psp5II	RGGWCCY	3 cut(s)	1486, 1587, 2041
Psp6I	CCWGG	5 cut(s)	93, 1008, 1482, 1610, 2043
PspGI	CCWGG	5 cut(s)	93, 1008, 1482, 1610, 2043
PspN4I	GGNNCC	8 cut(s)	984, 1124, 1588, 1589, 1790, 1839, 2042, 2192
PspPI	GGNCC	5 cut(s)	1122, 1486, 1587, 1660, 2041
PspPPI	RGGWCCY	3 cut(s)	1486, 1587, 2041
PstI	CTGCAG	1 cut(s)	2088
PsuI	RGATCY	2 cut(s)	90, 982
PsyI	GACNNNGTC	1 cut(s)	1214
PvuII	CAGCTG	4 cut(s)	614, 1527, 1634, 2083
RsaI	GTAC	9 cut(s)	71, 356, 912, 1206, 1704, 1904, 1924, 2186, 2192
RsaNI	GTAC	9 cut(s)	70, 355, 911, 1205, 1703, 1903, 1923, 2185, 2191
RseI	CAYNNNNRTG	2 cut(s)	350, 1400
SacI	GAGCTC	3 cut(s)	156, 818, 1453
SapI	GCTCTTC	1 cut(s)	285
SatI	GCNGC	7 cut(s)	182, 267, 953, 1001, 1528, 2010, 2084
Sau3AI	GATC	6 cut(s)	61, 90, 982, 2022, 2047, 2173
Sau96I	GGNCC	5 cut(s)	1122, 1486, 1587, 1660, 2041
ScaI	AGTACT	1 cut(s)	1904
SchI	GAGTC	5 cut(s)	641, 659, 723, 1952, 2141
ScrFI	CCNGG	7 cut(s)	95, 938, 1010, 1120, 1484, 1612, 2045
SduI	GDGCHC	6 cut(s)	10, 156, 383, 818, 1453, 1631
SfaNI	GCATC	5 cut(s)	55, 721, 1212, 1309, 1698
SfcI	CTRYAG	4 cut(s)	157, 987, 1758, 2084
SinI	GGWCC	4 cut(s)	1122, 1486, 1587, 2041
SmiMI	CAYNNNNRTG	2 cut(s)	350, 1400
SmlI	CTYRAG	2 cut(s)	479, 602
SmoI	CTYRAG	2 cut(s)	479, 602
SpeI	ACTAGT	1 cut(s)	799
SphI	GCATGC	1 cut(s)	104
SseBI	AGGCCT	1 cut(s)	38
SsiI	CCGC	8 cut(s)	135, 625, 927, 1322, 1359, 2038, 2066, 2146
SspI	AATATT	1 cut(s)	538
SspMI	CTAG	4 cut(s)	132, 735, 800, 956
SstI	GAGCTC	3 cut(s)	156, 818, 1453
StuI	AGGCCT	1 cut(s)	38
StyD4I	CCNGG	7 cut(s)	93, 936, 1008, 1118, 1482, 1610, 2043
TaaI	ACNGT	6 cut(s)	275, 571, 1309, 1444, 1480, 1927
TaiI	ACGT	2 cut(s)	86, 1739
TaqI	TCGA	4 cut(s)	147, 340, 2130, 2176
TatI	WGTACW	5 cut(s)	354, 1204, 1702, 1902, 2184
TfiI	GAWTC	5 cut(s)	106, 337, 1431, 2094, 2127
TscAI	CASTG	1 cut(s)	1402
TseFI	GTSAC	3 cut(s)	217, 785, 2071
TseI	GCWGC	7 cut(s)	181, 266, 952, 1000, 1527, 2009, 2083
Tsp45I	GTSAC	3 cut(s)	217, 785, 2071
TspGWI	ACGGA	2 cut(s)	1220, 2186
TspRI	CASTG	1 cut(s)	1402
Tth111I	GACNNNGTC	1 cut(s)	1214
Van91I	CCANNNNNTGG	1 cut(s)	727
VpaK11BI	GGWCC	4 cut(s)	1122, 1486, 1587, 2041
VspI	ATTAAT	1 cut(s)	1107
XapI	RAATTY	3 cut(s)	114, 188, 1084
XceI	RCATGY	1 cut(s)	104
XspI	CTAG	4 cut(s)	132, 735, 800, 956
ZrmI	AGTACT	1 cut(s)	1904
Zsp2I	ATGCAT	1 cut(s)	1428

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.