pycom17g04490

Belongs to the cullin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0003908
Physical Location & Seq
Forward (+)
3071005 .. 3073504
2500 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g04490.1

Sequence Viewer

Length: 1743 bp
ATGGATTTAGGTTCTATTGTTGACCAAGATGACTTTGAGAAGCATTTTCTTGAGGTTTCAGCTGATTTTTACCGCTATGAGTCTCAAGAGTTCATAGAGTCATGTGATTATGGGAACTATTTAAAGAAGGCTGATAGACGCTTAATGGAAGAAAGGGAGAGTGTGTCCCATTATTTGGATGCTAGAAGTGAAGCTAAGTTAACTATTATGGAGGAGAAGGTGATGATTGAAATTAATATGTTTATGGATGAAAAATATGACGACTTGGAAAGAATGTATAGCTTGTTTTGCAGGGTGCAGAATGGACTTGTAATAGTACAATATGTCATGACTGTTTACATCCGAGATACTGGTATGCAGCTAGTTACAGACCCAGAAAGGTTAAGGGATCCTATAGATTTTGTGCAGCGTCTCCTGGATTTGAAGGAAAAATATGACAAAGTTATCAGCTTGGTGTTTAACAATAACAAAACATTCCAAAATGCTCTAAATTCCTCTTTTGAATACGTCATCAATTTGAATGCCCTGTCCCCTAAATTCATTTCTCTGTTTGTCAATGACAAGCTTCTTAAAGGACTAAGAGGGGTTAGTGAGGAGGATGTAGAGGTTGTACTAGACAAGGTCATGATGTTAATGGATCCTGTGGATTTTGTGCAGCATCTCCTAGATTTAAAGGATAAATATGACAAGGTTATCAGCTTGGCGTTTAACAATGACAAAACATTCCAGAATGCTCTAAATTGTTCTTTTGAATACTTCATTAATTTGAATGCTCGGTCCCCTGAATTCATTTCTCTGTTTGTGGATAACAAGCTTCGGAAAGGACTGAGAGGGGTTAGTGAGGATGATGTAAAGGTTGTGCTTGATAAGGTCATGATGCTTGTCCGTTATCTTCAGGAGAAAGATGTCTTTGAAAAGTATTACAAGCAACATTTGGCAAATAGGTTTTTTTCTGGGAATACTGTTTCTGATGATGCAGAAAGAAGTCTGATTGTTAAGCTCAAAACAGAGTACGGATATCAGTTTACTTGTAAACTGGAGGGTATGTTCACTAATATGAAGATCTTTCAGGATACGATGCATGGATTCTACTCAACTGTGGGACCTCAGTTAGGCGATAACCCAATGCTAATTGTCCAGGTCCTCACGACAGGTTCATGGCCTACTCAACCAAGTGCCACGTACAACCTTCCAGCAAAAATTTTGTGGGTATGTGAGAAGTTCAAGAGTTACTATCTTGGGACCCATACTGGTCAGAGATTGTTGTGGCAAATTAATATGGGCACAACTGATTTAAAATCAACTTTTGGAAAGGGCCAGAAGCATGAGCTGAATGTTTCCATATATCAGTTGTGTGTACTGATGCTATTCAACAATGTTGATCGGTTGACGTATAAGGAAATTGAGCAGGCTACAGAGATACCAGCCGTTGACTTGAAGAGGTGCCTACAATCTCTGGCCTGTGTTAAAGGGAAGAACGTTTTTCGGAATGAGCCAATGAGCAAGGACATAGCTGAGGAGGATGCCTTCTTTTTTAATGAAAAGTTCACAAGCAAGTTCTTCAATATAAAGATTGGTATTGTGGTTGCCCAAAGGGAGACTGAACCAGAAAATGTAGAAACCCGACGGAGAGTAGAGGAAGACAGGAAGCCCCAGATTGAGGCAGCGATTGTGAGGATCATGAAGTCAAGGCGGGTCCTGGATCACAATAACATTGTTGCCGAGGTGACGAAACGGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 ko04340 ko04341 map04120 map04340 map04341
Pfam Domains
Protein Families
Cullin Cullin_Nedd8

Protein Analysis

581

Amino Acids

67.76

Weight (kDa)

5.58

Isoelectric Point (pI)

37.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin PF00888 9 - 212 1e-44 Cullin alpha solenoid domain
Cullin PF00888 213 - 360 2.3e-55 Cullin alpha solenoid domain
Cullin_AB PF26557 381 - 516 2.2e-43 Cullin alpha+beta domain
Cullin_Nedd8 PF10557 548 - 580 5.2e-10 Cullin protein neddylation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1443
AccB7I CCANNNNNTGG 1 cut(s) 175
AciI CCGC 2 cut(s) 73, 1693
AclI AACGTT 1 cut(s) 1479
AclWI GGATC 6 cut(s) 383, 396, 632, 645, 1685, 1710
AcsI RAATTY 4 cut(s) 490, 536, 785, 1200
AcuI CTGAAG 1 cut(s) 878
AfaI GTAC 5 cut(s) 318, 612, 1013, 1184, 1359
AfiI CCNNNNNNNGG 3 cut(s) 175, 1112, 1660
AjnI CCWGG 3 cut(s) 414, 1137, 1698
AloI GAACNNNNNNTCC 2 cut(s) 1031, 1063
Alw26I GTCTC 3 cut(s) 87, 416, 1592
AlwI GGATC 6 cut(s) 383, 396, 632, 645, 1685, 1710
AoxI GGCC 3 cut(s) 1160, 1315, 1458
ApeKI GCWGC 4 cut(s) 358, 406, 655, 1664
ApoI RAATTY 4 cut(s) 490, 536, 785, 1200
AseI ATTAAT 3 cut(s) 234, 762, 1275
AspS9I GGNCC 6 cut(s) 777, 1103, 1141, 1242, 1315, 1696
AsuHPI GGTGA 2 cut(s) 232, 1738
AvaII GGWCC 5 cut(s) 777, 1103, 1141, 1242, 1696
BaeGI GKGCMC 1 cut(s) 1286
BamHI GGATCC 2 cut(s) 388, 637
BanI GGYRCC 1 cut(s) 1443
BbsI GAAGAC 1 cut(s) 1647
BbvCI CCTCAGC 1 cut(s) 1515
BbvI GCAGC 4 cut(s) 370, 418, 667, 1676
BceAI ACGGC 1 cut(s) 1412
BciT130I CCWGG 3 cut(s) 416, 1139, 1700
BciVI GTATCC 1 cut(s) 1066
BcoDI GTCTC 3 cut(s) 87, 416, 1592
BfaI CTAG 4 cut(s) 183, 362, 614, 665
BfmI CTRYAG 3 cut(s) 393, 1413, 1739
BfuI GTATCC 1 cut(s) 1066
BglII AGATCT 1 cut(s) 1062
BisI GCNGC 4 cut(s) 359, 407, 656, 1665
BlsI GCNGC 4 cut(s) 360, 408, 657, 1666
Bme1390I CCNGG 3 cut(s) 416, 1139, 1700
Bme18I GGWCC 5 cut(s) 777, 1103, 1141, 1242, 1696
BmgT120I GGNCC 6 cut(s) 777, 1103, 1141, 1242, 1315, 1696
BmiI GGNNCC 8 cut(s) 390, 639, 779, 1104, 1243, 1244, 1445, 1697
BmrFI CCNGG 3 cut(s) 416, 1139, 1700
BmsI GCATC 7 cut(s) 169, 667, 867, 964, 1068, 1353, 1513
BpiI GAAGAC 1 cut(s) 1647
BpmI CTGGAG 1 cut(s) 1058
Bpu10I CCTNAGC 1 cut(s) 1515
BpuEI CTTGAG 2 cut(s) 69, 71
BsaAI YACGTR 1 cut(s) 1182
BsaJI CCNNGG 1 cut(s) 1722
BsaXI ACNNNNNCTCC 4 cut(s) 149, 179, 1031, 1061
Bsc4I CCNNNNNNNGG 3 cut(s) 175, 1112, 1660
Bse1I ACTGG 3 cut(s) 355, 1041, 1255
BseBI CCWGG 3 cut(s) 416, 1139, 1700
BseDI CCNNGG 1 cut(s) 1722
BseGI GGATG 6 cut(s) 184, 253, 339, 604, 850, 1528
BseLI CCNNNNNNNGG 3 cut(s) 175, 1112, 1660
BseMII CTCAG 3 cut(s) 818, 1121, 1506
BseNI ACTGG 3 cut(s) 355, 1041, 1255
BseRI GAGGAG 3 cut(s) 227, 608, 1532
BseSI GKGCMC 1 cut(s) 1286
BseXI GCAGC 4 cut(s) 370, 418, 667, 1676
BsgI GTGCAG 3 cut(s) 317, 425, 674
BshFI GGCC 3 cut(s) 1162, 1317, 1460
BshNI GGYRCC 1 cut(s) 1443
BslFI GGGAC 5 cut(s) 151, 514, 763, 1116, 1255
BslI CCNNNNNNNGG 3 cut(s) 175, 1112, 1660
BsmAI GTCTC 3 cut(s) 87, 416, 1592
BsmBI CGTCTC 1 cut(s) 416
BsmFI GGGAC 5 cut(s) 151, 514, 763, 1116, 1255
BsmI GAATGC 3 cut(s) 526, 736, 775
BsnI GGCC 3 cut(s) 1162, 1317, 1460
Bsp1286I GDGCHC 1 cut(s) 1286
Bsp143I GATC 6 cut(s) 388, 637, 1062, 1381, 1677, 1702
BspACI CCGC 2 cut(s) 73, 1693
BspANI GGCC 3 cut(s) 1162, 1317, 1460
BspCNI CTCAG 3 cut(s) 819, 1120, 1507
BspHI TCATGA 4 cut(s) 327, 624, 873, 1680
BspLI GGNNCC 8 cut(s) 390, 639, 779, 1104, 1243, 1244, 1445, 1697
BspPI GGATC 6 cut(s) 383, 396, 632, 645, 1685, 1710
BspT107I GGYRCC 1 cut(s) 1443
BsrI ACTGG 3 cut(s) 355, 1041, 1255
BssECI CCNNGG 1 cut(s) 1722
BssMI GATC 6 cut(s) 388, 637, 1062, 1381, 1677, 1702
Bst2UI CCWGG 3 cut(s) 416, 1139, 1700
Bst4CI ACNGT 3 cut(s) 334, 964, 1099
Bst6I CTCTTC 1 cut(s) 1433
BstBAI YACGTR 1 cut(s) 1182
BstC8I GCNNGC 1 cut(s) 1410
BstDEI CTNAG 5 cut(s) 195, 578, 827, 1107, 1515
BstENI CCTNNNNNAGG 1 cut(s) 1110
BstF5I GGATG 6 cut(s) 184, 253, 339, 604, 850, 1528
BstKTI GATC 6 cut(s) 391, 640, 1065, 1384, 1680, 1705
BstMAI GTCTC 3 cut(s) 87, 416, 1592
BstMBI GATC 6 cut(s) 388, 637, 1062, 1381, 1677, 1702
BstMWI GCNNNNNNNGC 1 cut(s) 288
BstNI CCWGG 3 cut(s) 416, 1139, 1700
BstSCI CCNGG 3 cut(s) 414, 1137, 1698
BstSFI CTRYAG 3 cut(s) 393, 1413, 1739
BstSLI GKGCMC 1 cut(s) 1286
BstV1I GCAGC 4 cut(s) 370, 418, 667, 1676
BstV2I GAAGAC 1 cut(s) 1647
BstX2I RGATCY 3 cut(s) 388, 637, 1062
BstYI RGATCY 3 cut(s) 388, 637, 1062
BsuI GTATCC 1 cut(s) 1066
BsuRI GGCC 3 cut(s) 1162, 1317, 1460
BtsCI GGATG 6 cut(s) 184, 253, 339, 604, 850, 1528
Cac8I GCNNGC 1 cut(s) 1410
CciI TCATGA 4 cut(s) 327, 624, 873, 1680
Cfr13I GGNCC 6 cut(s) 777, 1103, 1141, 1242, 1315, 1696
CseI GACGC 2 cut(s) 147, 398
Csp6I GTAC 5 cut(s) 317, 611, 1012, 1183, 1358
CviAII CATG 8 cut(s) 102, 328, 625, 874, 1082, 1158, 1325, 1681
CviQI GTAC 5 cut(s) 317, 611, 1012, 1183, 1358
DdeI CTNAG 5 cut(s) 195, 578, 827, 1107, 1515
DpnI GATC 6 cut(s) 390, 639, 1064, 1383, 1679, 1704
DpnII GATC 6 cut(s) 388, 637, 1062, 1381, 1677, 1702
DraI TTTAAA 3 cut(s) 123, 672, 1296
Eam1104I CTCTTC 1 cut(s) 1433
EarI CTCTTC 1 cut(s) 1433
Eco32I GATATC 1 cut(s) 1019
Eco47I GGWCC 5 cut(s) 777, 1103, 1141, 1242, 1696
Eco57I CTGAAG 1 cut(s) 878
EcoNI CCTNNNNNAGG 1 cut(s) 1110
EcoO109I RGGNCCY 4 cut(s) 1103, 1141, 1242, 1696
EcoRI GAATTC 1 cut(s) 785
EcoRII CCWGG 3 cut(s) 414, 1137, 1698
EcoRV GATATC 1 cut(s) 1019
EcoT22I ATGCAT 1 cut(s) 1083
Esp3I CGTCTC 1 cut(s) 416
FaeI CATG 8 cut(s) 105, 331, 628, 877, 1085, 1161, 1328, 1684
FaqI GGGAC 5 cut(s) 151, 514, 763, 1116, 1255
FatI CATG 8 cut(s) 101, 327, 624, 873, 1081, 1157, 1324, 1680
FauI CCCGC 1 cut(s) 1686
Fnu4HI GCNGC 4 cut(s) 359, 407, 656, 1665
FokI GGATG 6 cut(s) 191, 260, 326, 611, 857, 1535
Fsp4HI GCNGC 4 cut(s) 359, 407, 656, 1665
FspBI CTAG 4 cut(s) 183, 362, 614, 665
GluI GCNGC 4 cut(s) 359, 407, 656, 1665
GsuI CTGGAG 1 cut(s) 1058
HaeIII GGCC 3 cut(s) 1162, 1317, 1460
HgaI GACGC 2 cut(s) 147, 398
Hin1II CATG 8 cut(s) 105, 331, 628, 877, 1085, 1161, 1328, 1684
HincII GTYRAC 4 cut(s) 22, 201, 1389, 1432
HindII GTYRAC 4 cut(s) 22, 201, 1389, 1432
HindIII AAGCTT 2 cut(s) 563, 812
HinfI GANTC 3 cut(s) 80, 98, 1086
HpaI GTTAAC 1 cut(s) 201
HphI GGTGA 2 cut(s) 232, 1738
Hpy188I TCNGA 6 cut(s) 344, 819, 970, 990, 1257, 1488
Hpy99I CGWCG 1 cut(s) 1629
HpyAV CCTTC 5 cut(s) 121, 211, 418, 1199, 1537
HpyCH4III ACNGT 3 cut(s) 334, 964, 1099
HpyCH4IV ACGT 4 cut(s) 507, 1181, 1391, 1479
HpyCH4V TGCA 7 cut(s) 291, 298, 358, 406, 655, 977, 1081
HpyF10VI GCNNNNNNNGC 1 cut(s) 288
HpyF3I CTNAG 5 cut(s) 195, 578, 827, 1107, 1515
HpySE526I ACGT 4 cut(s) 507, 1181, 1391, 1479
Hsp92II CATG 8 cut(s) 105, 331, 628, 877, 1085, 1161, 1328, 1684
KflI GGGWCCC 1 cut(s) 1242
KspAI GTTAAC 1 cut(s) 201
Kzo9I GATC 6 cut(s) 388, 637, 1062, 1381, 1677, 1702
Lsp1109I GCAGC 4 cut(s) 370, 418, 667, 1676
LweI GCATC 7 cut(s) 169, 667, 867, 964, 1068, 1353, 1513
MaeI CTAG 4 cut(s) 183, 362, 614, 665
MaeII ACGT 4 cut(s) 507, 1181, 1391, 1479
MaeIII GTNAC 3 cut(s) 364, 1229, 1726
MalI GATC 6 cut(s) 390, 639, 1064, 1383, 1679, 1704
MboI GATC 6 cut(s) 388, 637, 1062, 1381, 1677, 1702
MboII GAAGA 7 cut(s) 161, 884, 1072, 1450, 1486, 1552, 1652
MflI RGATCY 3 cut(s) 388, 637, 1062
MhlI GDGCHC 1 cut(s) 1286
MlyI GAGTC 2 cut(s) 89, 107
Mph1103I ATGCAT 1 cut(s) 1083
MslI CAYNNNNRTG 1 cut(s) 1055
MspA1I CMGCKG 1 cut(s) 62
MspR9I CCNGG 3 cut(s) 416, 1139, 1700
Mva1269I GAATGC 3 cut(s) 526, 736, 775
MvaI CCWGG 3 cut(s) 416, 1139, 1700
MwoI GCNNNNNNNGC 1 cut(s) 288
NdeII GATC 6 cut(s) 388, 637, 1062, 1381, 1677, 1702
NlaIII CATG 8 cut(s) 105, 331, 628, 877, 1085, 1161, 1328, 1684
NlaIV GGNNCC 8 cut(s) 390, 639, 779, 1104, 1243, 1244, 1445, 1697
NmuCI GTSAC 1 cut(s) 1726
NsiI ATGCAT 1 cut(s) 1083
PagI TCATGA 4 cut(s) 327, 624, 873, 1680
PctI GAATGC 3 cut(s) 526, 736, 775
PfeI GAWTC 1 cut(s) 1086
PflFI GACNNNGTC 1 cut(s) 620
PflMI CCANNNNNTGG 1 cut(s) 175
PfoI TCCNGGA 2 cut(s) 414, 1698
PkrI GCNGC 4 cut(s) 360, 408, 657, 1666
PleI GAGTC 2 cut(s) 88, 106
PpsI GAGTC 2 cut(s) 88, 106
Ppu21I YACGTR 1 cut(s) 1182
PpuMI RGGWCCY 4 cut(s) 1103, 1141, 1242, 1696
PshBI ATTAAT 3 cut(s) 234, 762, 1275
Psp1406I AACGTT 1 cut(s) 1479
Psp5II RGGWCCY 4 cut(s) 1103, 1141, 1242, 1696
Psp6I CCWGG 3 cut(s) 414, 1137, 1698
PspGI CCWGG 3 cut(s) 414, 1137, 1698
PspN4I GGNNCC 8 cut(s) 390, 639, 779, 1104, 1243, 1244, 1445, 1697
PspPI GGNCC 6 cut(s) 777, 1103, 1141, 1242, 1315, 1696
PspPPI RGGWCCY 4 cut(s) 1103, 1141, 1242, 1696
PsuI RGATCY 3 cut(s) 388, 637, 1062
PsyI GACNNNGTC 1 cut(s) 620
PvuII CAGCTG 1 cut(s) 62
RsaI GTAC 5 cut(s) 318, 612, 1013, 1184, 1359
RsaNI GTAC 5 cut(s) 317, 611, 1012, 1183, 1358
RseI CAYNNNNRTG 1 cut(s) 1055
SatI GCNGC 4 cut(s) 359, 407, 656, 1665
Sau3AI GATC 6 cut(s) 388, 637, 1062, 1381, 1677, 1702
Sau96I GGNCC 6 cut(s) 777, 1103, 1141, 1242, 1315, 1696
SchI GAGTC 2 cut(s) 89, 107
ScrFI CCNGG 3 cut(s) 416, 1139, 1700
SduI GDGCHC 1 cut(s) 1286
SfaNI GCATC 7 cut(s) 169, 667, 867, 964, 1068, 1353, 1513
SfcI CTRYAG 3 cut(s) 393, 1413, 1739
SinI GGWCC 5 cut(s) 777, 1103, 1141, 1242, 1696
SmiMI CAYNNNNRTG 1 cut(s) 1055
SmlI CTYRAG 2 cut(s) 50, 84
SmoI CTYRAG 2 cut(s) 50, 84
SsiI CCGC 2 cut(s) 73, 1693
SspMI CTAG 4 cut(s) 183, 362, 614, 665
StyD4I CCNGG 3 cut(s) 414, 1137, 1698
TaaI ACNGT 3 cut(s) 334, 964, 1099
TaiI ACGT 4 cut(s) 510, 1184, 1394, 1482
TaqII GACCGA 1 cut(s) 765
TatI WGTACW 3 cut(s) 316, 610, 1357
TfiI GAWTC 1 cut(s) 1086
TseFI GTSAC 1 cut(s) 1726
TseI GCWGC 4 cut(s) 358, 406, 655, 1664
Tsp45I GTSAC 1 cut(s) 1726
TspDTI ATGAA 9 cut(s) 82, 264, 529, 748, 778, 1073, 1146, 1554, 1697
TspGWI ACGGA 3 cut(s) 875, 1029, 1642
Tth111I GACNNNGTC 1 cut(s) 620
Van91I CCANNNNNTGG 1 cut(s) 175
VpaK11BI GGWCC 5 cut(s) 777, 1103, 1141, 1242, 1696
VspI ATTAAT 3 cut(s) 234, 762, 1275
XagI CCTNNNNNAGG 1 cut(s) 1110
XapI RAATTY 4 cut(s) 490, 536, 785, 1200
XspI CTAG 4 cut(s) 183, 362, 614, 665
Zsp2I ATGCAT 1 cut(s) 1083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.