Rh4BG386400

Belongs to the cullin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003908
Physical Location & Seq
Reverse (-)
54031059 .. 54033291
2233 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG386400.1

Sequence Viewer

Length: 1389 bp
ATGGTGCTTCACAAATATGGTGAGAAGCTGTACTCGGGGCTGGTTAAAACTATGACTTCTCATCTCAAAGAAATATCCAAGTGTATTGAAGCTGCTCAAGGAGGGATGTTTCTGGAAGAGATGAATAAGTTGTGGACGGATCATAACAAGGCGTTGCAAATGATTCGAGACATACTGATGTACATGGACAGGACTTACATTCCGAGTACCCAGAAAACCCCTGTTCACGAGCTTGGGCTGAATCTTTGGAGAGATAACATTATTCACTCGAGCAAGATCCAGACGAGGCTTCTAAACACACTTCTTGAACTAGTACTCAGAGAACGGACCGGTGATGTTATCAACCGGGGTTTGATGAGGAATATAATTAAGATGCTAATGGACTTGGGCCCTTCAGTTTACCAGGAAGACTTTGAGAATCCATTTCTTGAGGTTTCAGCCGAATTTTACAAGGGTGAATCTCAGAAATTTATTGAGTGCTGTGATTGTGGGGATTATCTGAAGAAGGCTGAGAGGCGCCTAAATGAGGAACTGGACAGGGTGACACATTACTTGGATGCAAAGAGTGAGGTCAAGATTACTAATGTGGTCGAGAAGGAGATGATTGCTAACCACATGCTGCGACTAGTCCACATGGAGAATTCAGGCTTGGTGAACATGCTTCTTGATGATAAGTACGAAGATCTTGGAAGAATGTACAACTTGTTTCGTCGGGTTGCCAATGGTCTCTCAACAATACGAGAAGTGATGACTTCTCACATCAGGGAAACTGGTAAACAGCTGGTTACTGATCCAGAAAGGTTGAAGGATCCAGTGGAATTTGTGCAGAGGCTCTTGGATGAGAAAGATAAGTATGATAGCATCATTAAATTGTCGTTCAACAATGACAAGACATTCCAGAATGGTTTGAATTCCTCTTTTGAGTATTTTATTAATCTAAATACTCGTTCACCTGAGTTCATTTCACTGTTTGTTGATGACAAACTCCGCAAAGGGTTGAAGGGGGTCAGTGAGGAGGACGTAGAAGTTATTCTCGACAAGGTGATGATGTTGTTCCGTTACTTGCAGGAGAAAGATGTGTTTGAGAAATATTATAAACAGCATTTGGCAAAGAGGCTTTTGTCCGGTAAAACTGTCTCTGATGATGCAGAGAGAAGTTTAATAGTCAAGCTCAAGACAGAATGTGGGTACCAATTTACTTCAAAATTAGAGGGTATGTTTACAGATATGAAAACTTCCCAGGATACGATGCAAGGGTTTTATGCTAGCCATCCTGAGCTAGGGGATGGCCCTACATTGACCGTCCAGGTTCTAACAACGGGGTCCTGGCCCACTCAACCGAGCGTGACATGCAACCTTCCAGCAGAAATGTCAGCGCTCTATATATAA

Protein Analysis

462

Amino Acids

53.56

Weight (kDa)

5.87

Isoelectric Point (pI)

40.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin PF00888 1 - 417 2.7e-150 Cullin alpha solenoid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1095
Acc65I GGTACC 1 cut(s) 1188
AccB1I GGYRCC 2 cut(s) 516, 1188
AciI CCGC 1 cut(s) 988
AclWI GGATC 5 cut(s) 147, 271, 785, 803, 816
AcsI RAATTY 5 cut(s) 443, 467, 640, 818, 910
AcuI CTGAAG 2 cut(s) 378, 521
AcyI GRCGYC 1 cut(s) 517
AfaI GTAC 7 cut(s) 32, 182, 208, 315, 677, 698, 1190
AfeI AGCGCT 1 cut(s) 1377
AfiI CCNNNNNNNGG 2 cut(s) 526, 1280
AgeI ACCGGT 1 cut(s) 329
AgsI TTSAA 7 cut(s) 89, 308, 805, 880, 910, 1000, 1203
AhlI ACTAGT 2 cut(s) 310, 625
AjnI CCWGG 4 cut(s) 402, 1239, 1305, 1325
AjuI GAANNNNNNNTTGG 2 cut(s) 632, 664
AluBI AGCT 6 cut(s) 28, 92, 232, 781, 1171, 1279
AluI AGCT 6 cut(s) 28, 92, 232, 781, 1171, 1279
Alw26I GTCTC 3 cut(s) 162, 731, 1141
AlwI GGATC 5 cut(s) 147, 271, 785, 803, 816
Ama87I CYCGRG 2 cut(s) 34, 268
Aor51HI AGCGCT 1 cut(s) 1377
AoxI GGCC 3 cut(s) 388, 1288, 1328
ApaI GGGCCC 1 cut(s) 392
ApeKI GCWGC 2 cut(s) 92, 619
ApoI RAATTY 5 cut(s) 443, 467, 640, 818, 910
AseI ATTAAT 1 cut(s) 933
AsiGI ACCGGT 1 cut(s) 329
Asp700I GAANNNNTTC 1 cut(s) 1029
Asp718I GGTACC 1 cut(s) 1188
AspLEI GCGC 2 cut(s) 519, 1378
AspS9I GGNCC 6 cut(s) 327, 388, 389, 1289, 1323, 1329
AsuC2I CCSGG 1 cut(s) 347
AsuHPI GGTGA 7 cut(s) 32, 344, 467, 553, 664, 942, 1054
AsuNHI GCTAGC 1 cut(s) 1265
AvaI CYCGRG 2 cut(s) 34, 268
AvaII GGWCC 2 cut(s) 327, 1323
BaeGI GKGCMC 1 cut(s) 392
BamHI GGATCC 1 cut(s) 808
BanI GGYRCC 2 cut(s) 516, 1188
BanII GRGCYC 1 cut(s) 392
BauI CACGAG 1 cut(s) 227
BbsI GAAGAC 1 cut(s) 414
BbvI GCAGC 2 cut(s) 79, 606
BccI CCATC 2 cut(s) 1278, 1280
BcgI CGANNNNNNTGC 2 cut(s) 146, 180
BciT130I CCWGG 4 cut(s) 404, 1241, 1307, 1327
BciVI GTATCC 1 cut(s) 1237
BcnI CCSGG 1 cut(s) 347
BcoDI GTCTC 3 cut(s) 162, 731, 1141
BcuI ACTAGT 2 cut(s) 310, 625
BfaI CTAG 4 cut(s) 311, 626, 1266, 1280
BfoI RGCGCY 2 cut(s) 520, 1379
BfuI GTATCC 1 cut(s) 1237
BglII AGATCT 1 cut(s) 682
BisI GCNGC 2 cut(s) 93, 620
BlsI GCNGC 2 cut(s) 94, 621
BmcAI AGTACT 1 cut(s) 315
Bme1390I CCNGG 5 cut(s) 347, 404, 1241, 1307, 1327
Bme18I GGWCC 2 cut(s) 327, 1323
BmeT110I CYCGRG 2 cut(s) 34, 268
BmgT120I GGNCC 6 cut(s) 327, 388, 389, 1289, 1323, 1329
BmiI GGNNCC 5 cut(s) 390, 518, 810, 1190, 1324
BmrFI CCNGG 5 cut(s) 347, 404, 1241, 1307, 1327
BmsI GCATC 5 cut(s) 363, 547, 870, 1135, 1239
BmtI GCTAGC 1 cut(s) 1269
BpiI GAAGAC 1 cut(s) 414
Bpu10I CCTNAGC 1 cut(s) 1275
BpuEI CTTGAG 3 cut(s) 81, 449, 1157
BpuMI CCSGG 1 cut(s) 347
BsaHI GRCGYC 1 cut(s) 517
BsaI GGTCTC 1 cut(s) 731
BsaJI CCNNGG 2 cut(s) 346, 1239
BsaWI WCCGGW 2 cut(s) 329, 1124
Bsc4I CCNNNNNNNGG 2 cut(s) 526, 1280
Bse118I RCCGGY 1 cut(s) 329
Bse1I ACTGG 3 cut(s) 537, 775, 812
BseBI CCWGG 4 cut(s) 404, 1241, 1307, 1327
BseDI CCNNGG 2 cut(s) 346, 1239
BseGI GGATG 5 cut(s) 111, 562, 844, 1270, 1291
BseLI CCNNNNNNNGG 2 cut(s) 526, 1280
BseMII CTCAG 5 cut(s) 331, 476, 501, 945, 1266
BseNI ACTGG 3 cut(s) 537, 775, 812
BseRI GAGGAG 1 cut(s) 1028
BseSI GKGCMC 1 cut(s) 392
BseXI GCAGC 2 cut(s) 79, 606
BsgI GTGCAG 1 cut(s) 845
BshFI GGCC 3 cut(s) 390, 1290, 1330
BshNI GGYRCC 2 cut(s) 516, 1188
BshTI ACCGGT 1 cut(s) 329
BsiHKCI CYCGRG 2 cut(s) 34, 268
BsiSI CCGG 3 cut(s) 330, 346, 1125
BslI CCNNNNNNNGG 2 cut(s) 526, 1280
BsmAI GTCTC 3 cut(s) 162, 731, 1141
BsnI GGCC 3 cut(s) 390, 1290, 1330
Bso31I GGTCTC 1 cut(s) 731
BsoBI CYCGRG 2 cut(s) 34, 268
Bsp120I GGGCCC 1 cut(s) 388
Bsp1286I GDGCHC 1 cut(s) 392
Bsp1407I TGTACA 2 cut(s) 180, 696
Bsp143I GATC 5 cut(s) 139, 276, 682, 790, 808
BspACI CCGC 1 cut(s) 988
BspANI GGCC 3 cut(s) 390, 1290, 1330
BspCNI CTCAG 5 cut(s) 330, 475, 502, 946, 1267
BspLI GGNNCC 5 cut(s) 390, 518, 810, 1190, 1324
BspOI GCTAGC 1 cut(s) 1269
BspPI GGATC 5 cut(s) 147, 271, 785, 803, 816
BspT107I GGYRCC 2 cut(s) 516, 1188
BspTNI GGTCTC 1 cut(s) 731
BsrFI RCCGGY 1 cut(s) 329
BsrGI TGTACA 2 cut(s) 180, 696
BsrI ACTGG 3 cut(s) 537, 775, 812
BssAI RCCGGY 1 cut(s) 329
BssECI CCNNGG 2 cut(s) 346, 1239
BssMI GATC 5 cut(s) 139, 276, 682, 790, 808
BssNI GRCGYC 1 cut(s) 517
BssSI CACGAG 1 cut(s) 227
Bst2BI CACGAG 1 cut(s) 227
Bst2UI CCWGG 4 cut(s) 404, 1241, 1307, 1327
Bst4CI ACNGT 3 cut(s) 969, 1135, 1303
Bst6I CTCTTC 1 cut(s) 111
BstACI GRCGYC 1 cut(s) 517
BstAUI TGTACA 2 cut(s) 180, 696
BstC8I GCNNGC 1 cut(s) 1267
BstDEI CTNAG 5 cut(s) 317, 462, 510, 954, 1275
BstENI CCTNNNNNAGG 2 cut(s) 524, 1278
BstF5I GGATG 5 cut(s) 111, 562, 844, 1270, 1291
BstH2I RGCGCY 2 cut(s) 520, 1379
BstHHI GCGC 2 cut(s) 519, 1378
BstKTI GATC 5 cut(s) 142, 279, 685, 793, 811
BstMAI GTCTC 3 cut(s) 162, 731, 1141
BstMBI GATC 5 cut(s) 139, 276, 682, 790, 808
BstMWI GCNNNNNNNGC 1 cut(s) 1350
BstNI CCWGG 4 cut(s) 404, 1241, 1307, 1327
BstNSI RCATGY 3 cut(s) 619, 661, 1353
BstSCI CCNGG 5 cut(s) 345, 402, 1239, 1305, 1325
BstSLI GKGCMC 1 cut(s) 392
BstV1I GCAGC 2 cut(s) 79, 606
BstV2I GAAGAC 1 cut(s) 414
BstX2I RGATCY 3 cut(s) 276, 682, 808
BstYI RGATCY 3 cut(s) 276, 682, 808
BsuI GTATCC 1 cut(s) 1237
BsuRI GGCC 3 cut(s) 390, 1290, 1330
BtsCI GGATG 5 cut(s) 111, 562, 844, 1270, 1291
BtsIMutI CAGTG 3 cut(s) 819, 965, 1015
Cac8I GCNNGC 1 cut(s) 1267
CfoI GCGC 2 cut(s) 519, 1378
Cfr10I RCCGGY 1 cut(s) 329
Cfr13I GGNCC 6 cut(s) 327, 388, 389, 1289, 1323, 1329
CpoI CGGWCCG 1 cut(s) 327
Csp6I GTAC 7 cut(s) 31, 181, 207, 314, 676, 697, 1189
CspAI ACCGGT 1 cut(s) 329
CspI CGGWCCG 1 cut(s) 327
CviAII CATG 5 cut(s) 184, 616, 634, 658, 1350
CviQI GTAC 7 cut(s) 31, 181, 207, 314, 676, 697, 1189
DdeI CTNAG 5 cut(s) 317, 462, 510, 954, 1275
DinI GGCGCC 1 cut(s) 518
DpnI GATC 5 cut(s) 141, 278, 684, 792, 810
DpnII GATC 5 cut(s) 139, 276, 682, 790, 808
Eam1104I CTCTTC 1 cut(s) 111
EarI CTCTTC 1 cut(s) 111
Eco24I GRGCYC 1 cut(s) 392
Eco31I GGTCTC 1 cut(s) 731
Eco47I GGWCC 2 cut(s) 327, 1323
Eco47III AGCGCT 1 cut(s) 1377
Eco57I CTGAAG 2 cut(s) 378, 521
Eco88I CYCGRG 2 cut(s) 34, 268
EcoNI CCTNNNNNAGG 2 cut(s) 524, 1278
EcoO109I RGGNCCY 2 cut(s) 389, 1323
EcoRI GAATTC 2 cut(s) 640, 910
EcoRII CCWGG 4 cut(s) 402, 1239, 1305, 1325
EcoT38I GRGCYC 1 cut(s) 392
EgeI GGCGCC 1 cut(s) 518
EheI GGCGCC 1 cut(s) 518
FaeI CATG 5 cut(s) 187, 619, 637, 661, 1353
FatI CATG 5 cut(s) 183, 615, 633, 657, 1349
Fnu4HI GCNGC 2 cut(s) 93, 620
FokI GGATG 5 cut(s) 118, 569, 851, 1257, 1298
FriOI GRGCYC 1 cut(s) 392
Fsp4HI GCNGC 2 cut(s) 93, 620
FspBI CTAG 4 cut(s) 311, 626, 1266, 1280
GlaI GCGC 2 cut(s) 518, 1377
GluI GCNGC 2 cut(s) 93, 620
HaeII RGCGCY 2 cut(s) 520, 1379
HaeIII GGCC 3 cut(s) 390, 1290, 1330
HapII CCGG 3 cut(s) 330, 346, 1125
HhaI GCGC 2 cut(s) 519, 1378
Hin1I GRCGYC 1 cut(s) 517
Hin1II CATG 5 cut(s) 187, 619, 637, 661, 1353
Hin6I GCGC 2 cut(s) 517, 1376
HinP1I GCGC 2 cut(s) 517, 1376
HinfI GANTC 4 cut(s) 163, 241, 418, 458
HpaII CCGG 3 cut(s) 330, 346, 1125
HphI GGTGA 7 cut(s) 32, 344, 467, 553, 664, 942, 1054
Hpy166II GTNNAC 8 cut(s) 135, 226, 400, 631, 655, 776, 950, 1221
Hpy188I TCNGA 5 cut(s) 204, 320, 465, 501, 1141
Hpy8I GTNNAC 8 cut(s) 135, 226, 400, 631, 655, 776, 950, 1221
Hpy99I CGWCG 1 cut(s) 714
HpyAV CCTTC 6 cut(s) 402, 499, 589, 799, 994, 1367
HpyCH4III ACNGT 3 cut(s) 969, 1135, 1303
HpyCH4IV ACGT 1 cut(s) 1020
HpyCH4V TGCA 7 cut(s) 157, 560, 826, 1066, 1148, 1252, 1353
HpyF10VI GCNNNNNNNGC 1 cut(s) 1350
HpyF3I CTNAG 5 cut(s) 317, 462, 510, 954, 1275
HpySE526I ACGT 1 cut(s) 1020
Hsp92I GRCGYC 1 cut(s) 517
Hsp92II CATG 5 cut(s) 187, 619, 637, 661, 1353
HspAI GCGC 2 cut(s) 517, 1376
KasI GGCGCC 1 cut(s) 516
KpnI GGTACC 1 cut(s) 1192
Kzo9I GATC 5 cut(s) 139, 276, 682, 790, 808
Lsp1109I GCAGC 2 cut(s) 79, 606
LweI GCATC 5 cut(s) 363, 547, 870, 1135, 1239
MaeI CTAG 4 cut(s) 311, 626, 1266, 1280
MaeII ACGT 1 cut(s) 1020
MaeIII GTNAC 4 cut(s) 541, 784, 1058, 1345
MalI GATC 5 cut(s) 141, 278, 684, 792, 810
MboI GATC 5 cut(s) 139, 276, 682, 790, 808
MboII GAAGA 5 cut(s) 128, 419, 514, 692, 702
MflI RGATCY 3 cut(s) 276, 682, 808
MhlI GDGCHC 1 cut(s) 392
MluCI AATT 9 cut(s) 366, 443, 467, 640, 818, 869, 910, 1193, 1205
Mly113I GGCGCC 1 cut(s) 517
MroXI GAANNNNTTC 1 cut(s) 1029
MseI TTAA 5 cut(s) 45, 369, 867, 933, 1160
MslI CAYNNNNRTG 2 cut(s) 15, 176
MspA1I CMGCKG 1 cut(s) 781
MspI CCGG 3 cut(s) 330, 346, 1125
MspR9I CCNGG 5 cut(s) 347, 404, 1241, 1307, 1327
MvaI CCWGG 4 cut(s) 404, 1241, 1307, 1327
MwoI GCNNNNNNNGC 1 cut(s) 1350
NarI GGCGCC 1 cut(s) 517
NciI CCSGG 1 cut(s) 347
NdeII GATC 5 cut(s) 139, 276, 682, 790, 808
NheI GCTAGC 1 cut(s) 1265
NlaIII CATG 5 cut(s) 187, 619, 637, 661, 1353
NlaIV GGNNCC 5 cut(s) 390, 518, 810, 1190, 1324
NmuCI GTSAC 2 cut(s) 541, 1345
NspI RCATGY 3 cut(s) 619, 661, 1353
PaeR7I CTCGAG 1 cut(s) 268
PdmI GAANNNNTTC 1 cut(s) 1029
PfeI GAWTC 4 cut(s) 163, 241, 418, 458
PinAI ACCGGT 1 cut(s) 329
PkrI GCNGC 2 cut(s) 94, 621
PluTI GGCGCC 1 cut(s) 520
PpuMI RGGWCCY 1 cut(s) 1323
PshBI ATTAAT 1 cut(s) 933
PsiI TTATAA 1 cut(s) 1095
Psp5II RGGWCCY 1 cut(s) 1323
Psp6I CCWGG 4 cut(s) 402, 1239, 1305, 1325
PspGI CCWGG 4 cut(s) 402, 1239, 1305, 1325
PspN4I GGNNCC 5 cut(s) 390, 518, 810, 1190, 1324
PspOMI GGGCCC 1 cut(s) 388
PspPI GGNCC 6 cut(s) 327, 388, 389, 1289, 1323, 1329
PspPPI RGGWCCY 1 cut(s) 1323
PspXI VCTCGAGB 1 cut(s) 268
PsuI RGATCY 3 cut(s) 276, 682, 808
PvuII CAGCTG 1 cut(s) 781
RsaI GTAC 7 cut(s) 32, 182, 208, 315, 677, 698, 1190
RsaNI GTAC 7 cut(s) 31, 181, 207, 314, 676, 697, 1189
RseI CAYNNNNRTG 2 cut(s) 15, 176
Rsr2I CGGWCCG 1 cut(s) 327
RsrII CGGWCCG 1 cut(s) 327
SaqAI TTAA 5 cut(s) 45, 369, 867, 933, 1160
SatI GCNGC 2 cut(s) 93, 620
Sau3AI GATC 5 cut(s) 139, 276, 682, 790, 808
Sau96I GGNCC 6 cut(s) 327, 388, 389, 1289, 1323, 1329
ScaI AGTACT 1 cut(s) 315
ScrFI CCNGG 5 cut(s) 347, 404, 1241, 1307, 1327
SduI GDGCHC 1 cut(s) 392
SfaNI GCATC 5 cut(s) 363, 547, 870, 1135, 1239
SfoI GGCGCC 1 cut(s) 518
Sfr274I CTCGAG 1 cut(s) 268
SinI GGWCC 2 cut(s) 327, 1323
SlaI CTCGAG 1 cut(s) 268
SmiMI CAYNNNNRTG 2 cut(s) 15, 176
SmlI CTYRAG 4 cut(s) 96, 268, 428, 1172
SmoI CTYRAG 4 cut(s) 96, 268, 428, 1172
SpeI ACTAGT 2 cut(s) 310, 625
Sse9I AATT 9 cut(s) 366, 443, 467, 640, 818, 869, 910, 1193, 1205
SsiI CCGC 1 cut(s) 988
SspDI GGCGCC 1 cut(s) 516
SspI AATATT 1 cut(s) 1091
SspMI CTAG 4 cut(s) 311, 626, 1266, 1280
StyD4I CCNGG 5 cut(s) 345, 402, 1239, 1305, 1325
TaaI ACNGT 3 cut(s) 969, 1135, 1303
TaiI ACGT 1 cut(s) 1023
TaqI TCGA 4 cut(s) 166, 269, 591, 1035
TasI AATT 9 cut(s) 366, 443, 467, 640, 818, 869, 910, 1193, 1205
TatI WGTACW 4 cut(s) 30, 180, 313, 696
TfiI GAWTC 4 cut(s) 163, 241, 418, 458
Tru1I TTAA 5 cut(s) 45, 369, 867, 933, 1160
Tru9I TTAA 5 cut(s) 45, 369, 867, 933, 1160
TscAI CASTG 3 cut(s) 819, 972, 1015
TseFI GTSAC 2 cut(s) 541, 1345
TseI GCWGC 2 cut(s) 92, 619
Tsp45I GTSAC 2 cut(s) 541, 1345
TspDTI ATGAA 3 cut(s) 137, 949, 1244
TspGWI ACGGA 3 cut(s) 152, 340, 1046
TspRI CASTG 3 cut(s) 819, 972, 1015
VpaK11BI GGWCC 2 cut(s) 327, 1323
VspI ATTAAT 1 cut(s) 933
XagI CCTNNNNNAGG 2 cut(s) 524, 1278
XapI RAATTY 5 cut(s) 443, 467, 640, 818, 910
XceI RCATGY 3 cut(s) 619, 661, 1353
XhoI CTCGAG 1 cut(s) 268
XmnI GAANNNNTTC 1 cut(s) 1029
XspI CTAG 4 cut(s) 311, 626, 1266, 1280
ZrmI AGTACT 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.