Rorug04G0323000

Belongs to the cullin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003908
Physical Location & Seq
Reverse (-)
49834762 .. 49835511
750 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0323000.1

Sequence Viewer

Length: 210 bp
ATGTCTGACTGGGGACCGGTGTTTATTGCGGTGGTGCTGTTCGTGCTGTTGTCGCCAGGTCTGCTGTTCCAGGTGCCGGGGCGGCACGGGTGCGTGGAGTTTGGAAGCTTTCACACGAGCGGCGCAGCCATAATGGTTCACTCCCTCCTCTACTTTGCTCTCATTTGCGTCTTCTTGTTGGCCATTAAGGTCCACTTTTACCTTGGTTAA

Protein Analysis

69

Amino Acids

7.55

Weight (kDa)

6.88

Isoelectric Point (pI)

23.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3339 PF11820 1 - 67 5e-34 Protein of unknown function (DUF3339)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 73
AccBSI CCGCTC 1 cut(s) 120
AciI CCGC 3 cut(s) 29, 82, 120
AcoI YGGCCR 1 cut(s) 180
AfiI CCNNNNNNNGG 1 cut(s) 76
AgeI ACCGGT 1 cut(s) 16
AjnI CCWGG 2 cut(s) 55, 69
AluBI AGCT 1 cut(s) 108
AluI AGCT 1 cut(s) 108
AoxI GGCC 1 cut(s) 180
ApeKI GCWGC 1 cut(s) 125
AsiGI ACCGGT 1 cut(s) 16
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 2 cut(s) 14, 190
AsuC2I CCSGG 1 cut(s) 78
AvaII GGWCC 2 cut(s) 14, 190
BalI TGGCCA 1 cut(s) 182
BanI GGYRCC 1 cut(s) 73
BauI CACGAG 1 cut(s) 115
BbsI GAAGAC 1 cut(s) 163
BbvI GCAGC 1 cut(s) 137
BciT130I CCWGG 2 cut(s) 57, 71
BcnI CCSGG 1 cut(s) 78
BglI GCCNNNNNGGC 1 cut(s) 82
BisI GCNGC 3 cut(s) 83, 121, 126
BlsI GCNGC 3 cut(s) 84, 122, 127
Bme1390I CCNGG 3 cut(s) 57, 71, 78
Bme18I GGWCC 2 cut(s) 14, 190
BmgT120I GGNCC 2 cut(s) 14, 190
BmiI GGNNCC 2 cut(s) 15, 75
BmrFI CCNGG 3 cut(s) 57, 71, 78
BmrI ACTGGG 1 cut(s) 19
BmuI ACTGGG 1 cut(s) 19
BpiI GAAGAC 1 cut(s) 163
BpuMI CCSGG 1 cut(s) 78
BsaJI CCNNGG 2 cut(s) 77, 202
BsaWI WCCGGW 1 cut(s) 16
Bsc4I CCNNNNNNNGG 1 cut(s) 76
Bse118I RCCGGY 1 cut(s) 16
Bse1I ACTGG 1 cut(s) 14
BseBI CCWGG 2 cut(s) 57, 71
BseDI CCNNGG 2 cut(s) 77, 202
BseLI CCNNNNNNNGG 1 cut(s) 76
BseNI ACTGG 1 cut(s) 14
BseRI GAGGAG 1 cut(s) 137
BseXI GCAGC 1 cut(s) 137
BshFI GGCC 1 cut(s) 182
BshNI GGYRCC 1 cut(s) 73
BshTI ACCGGT 1 cut(s) 16
BsiSI CCGG 2 cut(s) 17, 77
BslFI GGGAC 1 cut(s) 27
BslI CCNNNNNNNGG 1 cut(s) 76
BsmFI GGGAC 1 cut(s) 27
BsnI GGCC 1 cut(s) 182
BspACI CCGC 3 cut(s) 29, 82, 120
BspANI GGCC 1 cut(s) 182
BspLI GGNNCC 2 cut(s) 15, 75
BspT107I GGYRCC 1 cut(s) 73
BsrBI CCGCTC 1 cut(s) 120
BsrFI RCCGGY 1 cut(s) 16
BsrI ACTGG 1 cut(s) 14
BssAI RCCGGY 1 cut(s) 16
BssECI CCNNGG 2 cut(s) 77, 202
BssSI CACGAG 1 cut(s) 115
BssT1I CCWWGG 1 cut(s) 202
Bst2BI CACGAG 1 cut(s) 115
Bst2UI CCWGG 2 cut(s) 57, 71
BstHHI GCGC 1 cut(s) 125
BstMWI GCNNNNNNNGC 4 cut(s) 43, 52, 61, 82
BstNI CCWGG 2 cut(s) 57, 71
BstSCI CCNGG 3 cut(s) 55, 69, 76
BstV1I GCAGC 1 cut(s) 137
BstV2I GAAGAC 1 cut(s) 163
BsuRI GGCC 1 cut(s) 182
CfoI GCGC 1 cut(s) 125
Cfr10I RCCGGY 1 cut(s) 16
Cfr13I GGNCC 2 cut(s) 14, 190
CseI GACGC 1 cut(s) 157
CspAI ACCGGT 1 cut(s) 16
CviJI RGCY 3 cut(s) 108, 128, 182
CviKI_1 RGCY 3 cut(s) 108, 128, 182
EaeI YGGCCR 1 cut(s) 180
Eco130I CCWWGG 1 cut(s) 202
Eco47I GGWCC 2 cut(s) 14, 190
EcoRII CCWGG 2 cut(s) 55, 69
EcoT14I CCWWGG 1 cut(s) 202
ErhI CCWWGG 1 cut(s) 202
FaiI YATR 1 cut(s) 131
FalI AAGNNNNNCTT 1 cut(s) 179
FaqI GGGAC 1 cut(s) 27
Fnu4HI GCNGC 3 cut(s) 83, 121, 126
Fsp4HI GCNGC 3 cut(s) 83, 121, 126
GlaI GCGC 1 cut(s) 124
GluI GCNGC 3 cut(s) 83, 121, 126
HaeIII GGCC 1 cut(s) 182
HapII CCGG 2 cut(s) 17, 77
HgaI GACGC 1 cut(s) 157
HhaI GCGC 1 cut(s) 125
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HindIII AAGCTT 1 cut(s) 106
HpaII CCGG 2 cut(s) 17, 77
Hpy166II GTNNAC 2 cut(s) 139, 193
Hpy188I TCNGA 1 cut(s) 7
Hpy8I GTNNAC 2 cut(s) 139, 193
HpyF10VI GCNNNNNNNGC 4 cut(s) 43, 52, 61, 82
HspAI GCGC 1 cut(s) 123
LpnPI CCDG 6 cut(s) 30, 42, 56, 69, 83, 90
Lsp1109I GCAGC 1 cut(s) 137
MbiI CCGCTC 1 cut(s) 120
MboII GAAGA 1 cut(s) 163
MlsI TGGCCA 1 cut(s) 182
MluNI TGGCCA 1 cut(s) 182
MnlI CCTC 2 cut(s) 155, 158
Mox20I TGGCCA 1 cut(s) 182
MscI TGGCCA 1 cut(s) 182
MseI TTAA 2 cut(s) 186, 208
Msp20I TGGCCA 1 cut(s) 182
MspI CCGG 2 cut(s) 17, 77
MspR9I CCNGG 3 cut(s) 57, 71, 78
MvaI CCWGG 2 cut(s) 57, 71
MwoI GCNNNNNNNGC 4 cut(s) 43, 52, 61, 82
NciI CCSGG 1 cut(s) 78
NlaIV GGNNCC 2 cut(s) 15, 75
PinAI ACCGGT 1 cut(s) 16
PkrI GCNGC 3 cut(s) 84, 122, 127
Psp6I CCWGG 2 cut(s) 55, 69
PspGI CCWGG 2 cut(s) 55, 69
PspN4I GGNNCC 2 cut(s) 15, 75
PspPI GGNCC 2 cut(s) 14, 190
SaqAI TTAA 2 cut(s) 186, 208
SatI GCNGC 3 cut(s) 83, 121, 126
Sau96I GGNCC 2 cut(s) 14, 190
ScrFI CCNGG 3 cut(s) 57, 71, 78
SetI ASST 5 cut(s) 61, 75, 110, 192, 204
SinI GGWCC 2 cut(s) 14, 190
SsiI CCGC 3 cut(s) 29, 82, 120
StyD4I CCNGG 3 cut(s) 55, 69, 76
StyI CCWWGG 1 cut(s) 202
TauI GCSGC 2 cut(s) 85, 123
Tru1I TTAA 2 cut(s) 186, 208
Tru9I TTAA 2 cut(s) 186, 208
TseI GCWGC 1 cut(s) 125
VpaK11BI GGWCC 2 cut(s) 14, 190
XcmI CCANNNNNNNNNTGG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.