Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003908

Physical Location & Seq

Genomic Coordinates Reverse (-)

5207839 .. 5210783

Gene Structure 2945 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1074600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2199 bp

ATGAGTAACCAGAAGAAGAGGAATTTTCAGATAGAGGCGTTCAAGCACAGGGTGGTTGTGGATCCGAAGTACGCAGACAAGACGTGGAAGGTTCTGGAGCACGCAATCCGCGAGATTTACAATCACAATGCCAGTGGCCTCAGCTTCGAAGAGCTTTACAGAAATGCCTACAATATGGTGCTCCACAAATTTGGCGAGAAGCTTTACTCGGGACTGGTTGCAACCATGACTTCTCATCTCAAAGAGATATCTAAATCGATTGAATCTGCTCAGGGTGGAATGTTTCTTGAAGAAATGAATAAGAAATGGACTGAACACAACAAGGCGTTGCAGATGATTCGAGACATTCTGATGTACATGGACAGGACTTACATTCCGAGCACTCAGAAAACCCCAGTTCACGAGCTTGGGCTGAACCTTTGGAGAGACAACATTGTCCGCTCTAGCAAGATTCAGACGAGGCTTCTTAACACACTTCTTGAACTAGTGCTCAGAGAACGAACCGGTAATGTTATCAACCGGGGTTTGATGAGGAATATAATTAAGATGCTTATGGATTTAGGTCCTTCAGTTTACCGGGAGGACTTTGAGAATCATTTTCTTGAGGTTTCAGCTGAATTTTACAAGGGCGAGTCTCAAAAATTTATCGAGTGCTGCGATTGTGGGGATTATCTGCAGCAGGCTGAGAGGCGCCTGAATGAGGAACTGGAAAGGGTGACACATTACTTGGATGCCAAGAGTGAAGCCAAGATAACAAATGTGGTGGAGAAGGAGATGATTGCAAACCACATGCTGAGATTAGTCCACATGGAGAATTCAGGTTTGGTGAACATGCTTCTTGATGATAAGTATGAAGACCTTGGAAGAATGTACAACTTGTTCCGTCGGGTTGCCAACGGTCTCCCAACAATACGAGAAGTGATGACTTCTCACATCAGGGAAACTGGTAAACAGCTGGTTACCGATCCAGAAAGGTTGAAGGATCCAGTGGAGTTTGTCCAGAGGCTCTTGGATGAGAAAGATAAGTATGACAGCATTATTAGACTGTCTTTTAACAATGACAAGACATTCCTAAATGCTTTGAATTCCTCTTTCGAGTACTTCATTAATCTCAACAATCGTTCGCCTGAGTTCATTTCGCTGTTTGTTGATGACAAACTCCGCAAAAGTTTGAAGGGGGTCAGTGAGGAGGATGTAGAAATTATTCTCGACAAGGTGATGATGTTGTTCCGTTACTTGCAGGAGAAAGACGTATTTGAGAAGTACTATAAACAGCATTTGGCAAAGCGGCTTTTGTCTGGTAAAACTGTCTCCGACGATGCAGAGAGAAGTTTGATAGTTAAGCTCAAGACCGAATGCGGATACCAGTTTACTTCAAAATTAGAGGGCATGTTTACAGATATGAAAACCTCCCAGGATACAATGCAAGGGTTTTATGCAAGTCATCTCGAGCTAGAGGATGGCCCTACATTGACCGTCCAGGTTCTGACGACAGGGTCCTGGCCCACTCAACCTAGTGTGACATGCAACCTTCCATTAGAAATGTCGGAACTCTGTGAGAAGTTCCGTTCGTATTACCTCGGGACACATACAGGCCGGAGATTGTCCTGGCAGACTAACATGGGCACGGCAGATATAAAGGCTACCTTCGGGAAAGGCCAGAGGCATGAGTTGAATGTCTCCACTTACCAAATGTGTGTCCTAATGCTGTTTAATAATGCTGATCGACTGAACTATAAGGAGATTGAGCAGGCCGCGGAGATTCCCGCCTTAGATTTGAAGAGATGTCTGCAATCCATGGCTTGTGTGAAGGGAAAGAATGTTCTTCGGAAAGAGCCTATGAGTAAAGACATTGGCGAGGACGATGCTTTCTTTGTTAACGACAAGTTCACAAGTAAGTTGTATAAGGTGAAGATAGGAACTGTGGTGGCGCAGAAGGAATCAGAACCCGAAAAACAAGAGACTCGTCAGAGAGTGGAGGAGGACAGGAAGCCCCAGATCGAAGCTGCGATAGTGAGAATCATGAAATCCAGGAGGACTCTGGATCATAACAACGTAACATCCGAGGTCACAAAGCAGTTGCAATCTCGATTCCTGGCCAACCCAACGGAGATTAAGAAGCGGATTGAGTCCCTTATCGAGCGGGAGTTTTTGGAACGCGACAATGTAGACAGAAAATTGTATCGGTATCTTGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009909 GO:0009911 GO:0019899 GO:0031461 GO:0031625 GO:0032991 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0048518 GO:0048580 GO:0048582 GO:0048831 GO:0050789 GO:0050793 GO:0050896 GO:0051094 GO:0051239 GO:0051240 GO:0065007 GO:1902494 GO:1990234 GO:2000026 GO:2000241 GO:2000243

KEGG Pathways

Metabolic & Signaling

ko04120 ko04340 ko04341 map04120 map04340 map04341

Pfam Domains

Protein Families

Cullin Cullin_Nedd8

Protein Analysis

733

Amino Acids

85.56

Weight (kDa)

8.12

Isoelectric Point (pI)

45.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cullin	PF00888	29 - 475	1.7e-163	Cullin alpha solenoid domain
Cullin_AB	PF26557	495 - 630	6.7e-49	Cullin alpha+beta domain
Cullin_Nedd8	PF10557	662 - 722	7e-26	Cullin protein neddylation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003908)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G26830 AT1G69670 AT1G69670
fragaria_vesca	FvH4_4g31500 FvH4_6g48910
malus_domestica	MD09G1049800.v1.1 MD16G1074600.v1.1 MD17G1050100.v1.1
prunus_persica	Prupe.1G272800_v2.0.a1 Prupe.3G269000_v2.0.a1
pyrus_communis	pycom111g04080 pycom16g06460 pycom17g04490 pycom17g04500
rosa_chinensis	RchiOBHm_Chr2g0168721 RchiOBHm_Chr4g0439991
rosa_laevigata	RLG00000006217 RLG00000021804
rosa_multiflora	Rmu_sc0006000.1_g000007 Rmu_sc0034426.1_g000001
rosa_roxburghii	Rroxscaffold_2G00082740 Rroxscaffold_5G00380940
rosa_rugosa	Rorug02G0535000 Rorug04G0323000
rosa_samantha	Rh2AG605700 Rh2BG618700 Rh2CG586800 Rh2DG629200 Rh4AG374100 Rh4BG386400 Rh4CG400900 Rh4DG380700
rosa_wichuraiana	Rw2G050210 Rw4G032000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	434, 1495
AccB1I	GGYRCC	1 cut(s)	690
AccBSI	CCGCTC	2 cut(s)	441, 2143
AccI	GTMKAC	1 cut(s)	2169
AccII	CGCG	3 cut(s)	111, 1757, 2160
AclWI	GGATC	6 cut(s)	56, 69, 959, 977, 990, 2052
AcoI	YGGCCR	1 cut(s)	2097
AcsI	RAATTY	6 cut(s)	22, 188, 617, 641, 814, 1084
AcuI	CTGAAG	1 cut(s)	552
AcyI	GRCGYC	1 cut(s)	691
AdeI	CACNNNGTG	1 cut(s)	52
AfaI	GTAC	5 cut(s)	71, 356, 872, 1100, 1265
AfiI	CCNNNNNNNGG	1 cut(s)	700
AgeI	ACCGGT	1 cut(s)	503
AhlI	ACTAGT	1 cut(s)	484
AjiI	CACGTC	1 cut(s)	84
AjnI	CCWGG	6 cut(s)	1413, 1479, 1499, 1607, 2030, 2094
AjuI	GAANNNNNNNTTGG	2 cut(s)	806, 838
AloI	GAACNNNNNNTCC	2 cut(s)	1806, 1838
AluBI	AGCT	9 cut(s)	144, 154, 202, 406, 614, 955, 1345, 1453, 2006
AluI	AGCT	9 cut(s)	144, 154, 202, 406, 614, 955, 1345, 1453, 2006
Alw21I	GWGCWC	4 cut(s)	102, 183, 383, 492
Alw26I	GTCTC	7 cut(s)	336, 420, 639, 905, 1315, 1684, 1955
AlwI	GGATC	6 cut(s)	56, 69, 959, 977, 990, 2052
AlwNI	CAGNNNCTG	1 cut(s)	1486
Ama87I	CYCGRG	3 cut(s)	208, 1448, 1580
AoxI	GGCC	7 cut(s)	136, 1462, 1502, 1594, 1657, 1752, 2097
ApeKI	GCWGC	3 cut(s)	654, 676, 2006
ApoI	RAATTY	6 cut(s)	22, 188, 617, 641, 814, 1084
AseI	ATTAAT	1 cut(s)	1107
AsiGI	ACCGGT	1 cut(s)	503
Asp700I	GAANNNNTTC	1 cut(s)	1203
AspLEI	GCGC	2 cut(s)	693, 1933
AspS9I	GGNCC	4 cut(s)	563, 1463, 1497, 1503
AsuC2I	CCSGG	2 cut(s)	521, 578
AsuHPI	GGTGA	4 cut(s)	727, 838, 1228, 1921
AsuII	TTCGAA	1 cut(s)	147
AvaI	CYCGRG	3 cut(s)	208, 1448, 1580
AvaII	GGWCC	2 cut(s)	563, 1497
BaeGI	GKGCMC	1 cut(s)	1628
BalI	TGGCCA	1 cut(s)	2099
BamHI	GGATCC	2 cut(s)	61, 982
BanI	GGYRCC	1 cut(s)	690
BauI	CACGAG	1 cut(s)	401
BbsI	GAAGAC	1 cut(s)	861
Bbv12I	GWGCWC	4 cut(s)	102, 183, 383, 492
BbvCI	CCTCAGC	1 cut(s)	140
BbvI	GCAGC	3 cut(s)	641, 688, 1993
BccI	CCATC	1 cut(s)	1454
BceAI	ACGGC	1 cut(s)	1644
BcgI	CGANNNNNNTGC	4 cut(s)	320, 354, 1847, 1881
BciT130I	CCWGG	6 cut(s)	1415, 1481, 1501, 1609, 2032, 2096
BciVI	GTATCC	2 cut(s)	1355, 1411
BcnI	CCSGG	2 cut(s)	521, 578
BcoDI	GTCTC	7 cut(s)	336, 420, 639, 905, 1315, 1684, 1955
BcuI	ACTAGT	1 cut(s)	484
BfaI	CTAG	4 cut(s)	444, 485, 1454, 1515
BfmI	CTRYAG	1 cut(s)	674
BfoI	RGCGCY	1 cut(s)	694
BfuI	GTATCC	2 cut(s)	1355, 1411
BisI	GCNGC	5 cut(s)	655, 677, 1289, 1755, 2007
BlsI	GCNGC	5 cut(s)	656, 678, 1290, 1756, 2008
BmcAI	AGTACT	2 cut(s)	1100, 1265
Bme1390I	CCNGG	8 cut(s)	521, 578, 1415, 1481, 1501, 1609, 2032, 2096
Bme18I	GGWCC	2 cut(s)	563, 1497
BmeT110I	CYCGRG	3 cut(s)	208, 1448, 1580
BmgBI	CACGTC	1 cut(s)	84
BmgT120I	GGNCC	4 cut(s)	563, 1463, 1497, 1503
BmiI	GGNNCC	4 cut(s)	63, 692, 984, 1498
BmrFI	CCNGG	8 cut(s)	521, 578, 1415, 1481, 1501, 1609, 2032, 2096
BmrI	ACTGGG	1 cut(s)	389
BmsI	GCATC	4 cut(s)	537, 721, 1309, 1855
BmuI	ACTGGG	1 cut(s)	389
BpiI	GAAGAC	1 cut(s)	861
BpmI	CTGGAG	1 cut(s)	116
Bpu10I	CCTNAGC	2 cut(s)	140, 270
Bpu14I	TTCGAA	1 cut(s)	147
BpuEI	CTTGAG	2 cut(s)	623, 1331
BpuMI	CCSGG	2 cut(s)	521, 578
Bsa29I	ATCGAT	1 cut(s)	257
BsaHI	GRCGYC	1 cut(s)	691
BsaI	GGTCTC	1 cut(s)	905
BsaJI	CCNNGG	7 cut(s)	520, 859, 1413, 1579, 1755, 1797, 2064
BsaWI	WCCGGW	1 cut(s)	503
Bsc4I	CCNNNNNNNGG	1 cut(s)	700
Bse118I	RCCGGY	1 cut(s)	503
Bse1I	ACTGG	7 cut(s)	132, 219, 395, 711, 949, 986, 1366
BseBI	CCWGG	6 cut(s)	1415, 1481, 1501, 1609, 2032, 2096
BseCI	ATCGAT	1 cut(s)	257
BseDI	CCNNGG	7 cut(s)	520, 859, 1413, 1579, 1755, 1797, 2064
BseGI	GGATG	5 cut(s)	736, 1018, 1198, 1465, 2060
BseLI	CCNNNNNNNGG	1 cut(s)	700
BseMII	CTCAG	7 cut(s)	154, 284, 398, 505, 675, 785, 1119
BseNI	ACTGG	7 cut(s)	132, 219, 395, 711, 949, 986, 1366
BseRI	GAGGAG	2 cut(s)	1202, 1994
BseSI	GKGCMC	1 cut(s)	1628
BseXI	GCAGC	3 cut(s)	641, 688, 1993
Bsh1236I	CGCG	3 cut(s)	111, 1757, 2160
BshFI	GGCC	7 cut(s)	138, 1464, 1504, 1596, 1659, 1754, 2099
BshNI	GGYRCC	1 cut(s)	690
BshTI	ACCGGT	1 cut(s)	503
BshVI	ATCGAT	1 cut(s)	257
BsiHKAI	GWGCWC	4 cut(s)	102, 183, 383, 492
BsiHKCI	CYCGRG	3 cut(s)	208, 1448, 1580
BsiSI	CCGG	4 cut(s)	504, 520, 577, 1597
BslFI	GGGAC	3 cut(s)	225, 1597, 2116
BslI	CCNNNNNNNGG	1 cut(s)	700
BsmAI	GTCTC	7 cut(s)	336, 420, 639, 905, 1315, 1684, 1955
BsmFI	GGGAC	3 cut(s)	225, 1597, 2116
BsmI	GAATGC	1 cut(s)	1361
BsnI	GGCC	7 cut(s)	138, 1464, 1504, 1596, 1659, 1754, 2099
Bso31I	GGTCTC	1 cut(s)	905
BsoBI	CYCGRG	3 cut(s)	208, 1448, 1580
Bsp119I	TTCGAA	1 cut(s)	147
Bsp1286I	GDGCHC	5 cut(s)	102, 183, 383, 492, 1628
Bsp1407I	TGTACA	2 cut(s)	354, 870
Bsp143I	GATC	6 cut(s)	61, 964, 982, 1723, 1998, 2044
Bsp19I	CCATGG	1 cut(s)	1797
BspANI	GGCC	7 cut(s)	138, 1464, 1504, 1596, 1659, 1754, 2099
BspCNI	CTCAG	7 cut(s)	153, 283, 397, 504, 676, 786, 1120
BspDI	ATCGAT	1 cut(s)	257
BspFNI	CGCG	3 cut(s)	111, 1757, 2160
BspHI	TCATGA	1 cut(s)	2022
BspLI	GGNNCC	4 cut(s)	63, 692, 984, 1498
BspMAI	CTGCAG	1 cut(s)	678
BspPI	GGATC	6 cut(s)	56, 69, 959, 977, 990, 2052
BspQI	GCTCTTC	1 cut(s)	144
BspT104I	TTCGAA	1 cut(s)	147
BspT107I	GGYRCC	1 cut(s)	690
BspTNI	GGTCTC	1 cut(s)	905
BsrBI	CCGCTC	2 cut(s)	441, 2143
BsrFI	RCCGGY	1 cut(s)	503
BsrGI	TGTACA	2 cut(s)	354, 870
BsrI	ACTGG	7 cut(s)	132, 219, 395, 711, 949, 986, 1366
BssAI	RCCGGY	1 cut(s)	503
BssECI	CCNNGG	7 cut(s)	520, 859, 1413, 1579, 1755, 1797, 2064
BssMI	GATC	6 cut(s)	61, 964, 982, 1723, 1998, 2044
BssNI	GRCGYC	1 cut(s)	691
BssSI	CACGAG	1 cut(s)	401
BssT1I	CCWWGG	2 cut(s)	859, 1797
Bst2BI	CACGAG	1 cut(s)	401
Bst2UI	CCWGG	6 cut(s)	1415, 1481, 1501, 1609, 2032, 2096
Bst4CI	ACNGT	5 cut(s)	899, 1047, 1309, 1477, 1924
Bst6I	CTCTTC	3 cut(s)	11, 144, 1775
BstACI	GRCGYC	1 cut(s)	691
BstAUI	TGTACA	2 cut(s)	354, 870
BstBI	TTCGAA	1 cut(s)	147
BstC8I	GCNNGC	3 cut(s)	102, 681, 1752
BstDEI	CTNAG	8 cut(s)	140, 270, 384, 491, 684, 794, 1128, 1771
BstDSI	CCRYGG	2 cut(s)	1755, 1797
BstEII	GGTNACC	1 cut(s)	958
BstENI	CCTNNNNNAGG	1 cut(s)	698
BstF5I	GGATG	5 cut(s)	736, 1018, 1198, 1465, 2060
BstFNI	CGCG	3 cut(s)	111, 1757, 2160
BstH2I	RGCGCY	1 cut(s)	694
BstHHI	GCGC	2 cut(s)	693, 1933
BstKTI	GATC	6 cut(s)	64, 967, 985, 1726, 2001, 2047
BstMAI	GTCTC	7 cut(s)	336, 420, 639, 905, 1315, 1684, 1955
BstMBI	GATC	6 cut(s)	61, 964, 982, 1723, 1998, 2044
BstNI	CCWGG	6 cut(s)	1415, 1481, 1501, 1609, 2032, 2096
BstNSI	RCATGY	4 cut(s)	793, 835, 1393, 1527
BstPI	GGTNACC	1 cut(s)	958
BstSCI	CCNGG	8 cut(s)	519, 576, 1413, 1479, 1499, 1607, 2030, 2094
BstSFI	CTRYAG	1 cut(s)	674
BstSLI	GKGCMC	1 cut(s)	1628
BstUI	CGCG	3 cut(s)	111, 1757, 2160
BstV1I	GCAGC	3 cut(s)	641, 688, 1993
BstV2I	GAAGAC	1 cut(s)	861
BstX2I	RGATCY	2 cut(s)	61, 982
BstXI	CCANNNNNNTGG	1 cut(s)	191
BstYI	RGATCY	2 cut(s)	61, 982
Bsu15I	ATCGAT	1 cut(s)	257
BsuI	GTATCC	2 cut(s)	1355, 1411
BsuRI	GGCC	7 cut(s)	138, 1464, 1504, 1596, 1659, 1754, 2099
BsuTUI	ATCGAT	1 cut(s)	257
BtgI	CCRYGG	2 cut(s)	1755, 1797
BtrI	CACGTC	1 cut(s)	84
BtsCI	GGATG	5 cut(s)	736, 1018, 1198, 1465, 2060
BtsIMutI	CAGTG	3 cut(s)	139, 993, 1189
Cac8I	GCNNGC	3 cut(s)	102, 681, 1752
CaiI	CAGNNNCTG	1 cut(s)	1486
CciI	TCATGA	1 cut(s)	2022
CfoI	GCGC	2 cut(s)	693, 1933
Cfr10I	RCCGGY	1 cut(s)	503
Cfr13I	GGNCC	4 cut(s)	563, 1463, 1497, 1503
Cfr42I	CCGCGG	1 cut(s)	1758
ClaI	ATCGAT	1 cut(s)	257
Csp6I	GTAC	5 cut(s)	70, 355, 871, 1099, 1264
CspAI	ACCGGT	1 cut(s)	503
CspCI	CAANNNNNGTGG	4 cut(s)	115, 150, 744, 779
CviQI	GTAC	5 cut(s)	70, 355, 871, 1099, 1264
DdeI	CTNAG	8 cut(s)	140, 270, 384, 491, 684, 794, 1128, 1771
DinI	GGCGCC	1 cut(s)	692
DpnI	GATC	6 cut(s)	63, 966, 984, 1725, 2000, 2046
DpnII	GATC	6 cut(s)	61, 964, 982, 1723, 1998, 2044
DraIII	CACNNNGTG	1 cut(s)	52
DrdI	GACNNNNNNGTC	2 cut(s)	434, 1495
DseDI	GACNNNNNNGTC	2 cut(s)	434, 1495
EaeI	YGGCCR	1 cut(s)	2097
Eam1104I	CTCTTC	3 cut(s)	11, 144, 1775
EarI	CTCTTC	3 cut(s)	11, 144, 1775
Eco130I	CCWWGG	2 cut(s)	859, 1797
Eco31I	GGTCTC	1 cut(s)	905
Eco32I	GATATC	1 cut(s)	249
Eco47I	GGWCC	2 cut(s)	563, 1497
Eco57I	CTGAAG	1 cut(s)	552
Eco88I	CYCGRG	3 cut(s)	208, 1448, 1580
Eco91I	GGTNACC	1 cut(s)	958
EcoNI	CCTNNNNNAGG	1 cut(s)	698
EcoO109I	RGGNCCY	2 cut(s)	563, 1497
EcoO65I	GGTNACC	1 cut(s)	958
EcoRI	GAATTC	2 cut(s)	814, 1084
EcoRII	CCWGG	6 cut(s)	1413, 1479, 1499, 1607, 2030, 2094
EcoRV	GATATC	1 cut(s)	249
EcoT14I	CCWWGG	2 cut(s)	859, 1797
EgeI	GGCGCC	1 cut(s)	692
EheI	GGCGCC	1 cut(s)	692
ErhI	CCWWGG	2 cut(s)	859, 1797
FalI	AAGNNNNNCTT	2 cut(s)	1631, 1663
FaqI	GGGAC	3 cut(s)	225, 1597, 2116
FauI	CCCGC	2 cut(s)	1774, 2136
FblI	GTMKAC	1 cut(s)	2169
Fnu4HI	GCNGC	5 cut(s)	655, 677, 1289, 1755, 2007
FokI	GGATG	5 cut(s)	743, 1025, 1205, 1472, 2047
Fsp4HI	GCNGC	5 cut(s)	655, 677, 1289, 1755, 2007
FspBI	CTAG	4 cut(s)	444, 485, 1454, 1515
GlaI	GCGC	2 cut(s)	692, 1932
GluI	GCNGC	5 cut(s)	655, 677, 1289, 1755, 2007
GsuI	CTGGAG	1 cut(s)	116
HaeII	RGCGCY	1 cut(s)	694
HaeIII	GGCC	7 cut(s)	138, 1464, 1504, 1596, 1659, 1754, 2099
HapII	CCGG	4 cut(s)	504, 520, 577, 1597
HhaI	GCGC	2 cut(s)	693, 1933
Hin1I	GRCGYC	1 cut(s)	691
Hin6I	GCGC	2 cut(s)	691, 1931
HinP1I	GCGC	2 cut(s)	691, 1931
HincII	GTYRAC	1 cut(s)	1879
HindII	GTYRAC	1 cut(s)	1879
HindIII	AAGCTT	1 cut(s)	200
HpaI	GTTAAC	1 cut(s)	1879
HpaII	CCGG	4 cut(s)	504, 520, 577, 1597
HphI	GGTGA	4 cut(s)	727, 838, 1228, 1921
Hpy99I	CGWCG	2 cut(s)	888, 1319
HpyAV	CCTTC	9 cut(s)	82, 576, 763, 973, 1168, 1541, 1657, 1804, 1930
HpyCH4III	ACNGT	5 cut(s)	899, 1047, 1309, 1477, 1924
HpyCH4IV	ACGT	3 cut(s)	83, 1251, 2055
HpyF3I	CTNAG	8 cut(s)	140, 270, 384, 491, 684, 794, 1128, 1771
HpySE526I	ACGT	3 cut(s)	83, 1251, 2055
Hsp92I	GRCGYC	1 cut(s)	691
HspAI	GCGC	2 cut(s)	691, 1931
KasI	GGCGCC	1 cut(s)	690
KspAI	GTTAAC	1 cut(s)	1879
KspI	CCGCGG	1 cut(s)	1758
Kzo9I	GATC	6 cut(s)	61, 964, 982, 1723, 1998, 2044
LguI	GCTCTTC	1 cut(s)	144
LmnI	GCTCC	2 cut(s)	97, 186
Lsp1109I	GCAGC	3 cut(s)	641, 688, 1993
LweI	GCATC	4 cut(s)	537, 721, 1309, 1855
MaeI	CTAG	4 cut(s)	444, 485, 1454, 1515
MaeII	ACGT	3 cut(s)	83, 1251, 2055
MaeIII	GTNAC	7 cut(s)	5, 715, 958, 1232, 1519, 2056, 2068
MalI	GATC	6 cut(s)	63, 966, 984, 1725, 2000, 2046
MbiI	CCGCTC	2 cut(s)	441, 2143
MboI	GATC	6 cut(s)	61, 964, 982, 1723, 1998, 2044
MboII	GAAGA	9 cut(s)	25, 28, 161, 302, 866, 876, 1792, 1817, 1924
MflI	RGATCY	2 cut(s)	61, 982
MhlI	GDGCHC	5 cut(s)	102, 183, 383, 492, 1628
MlsI	TGGCCA	1 cut(s)	2099
MluNI	TGGCCA	1 cut(s)	2099
Mly113I	GGCGCC	1 cut(s)	691
MlyI	GAGTC	4 cut(s)	641, 1957, 2032, 2138
MmeI	TCCRAC	2 cut(s)	1338, 1527
Mox20I	TGGCCA	1 cut(s)	2099
MroXI	GAANNNNTTC	1 cut(s)	1203
MscI	TGGCCA	1 cut(s)	2099
MseI	TTAA	8 cut(s)	468, 543, 1053, 1107, 1341, 1713, 1878, 2115
MslI	CAYNNNNRTG	1 cut(s)	350
Msp20I	TGGCCA	1 cut(s)	2099
MspA1I	CMGCKG	3 cut(s)	614, 955, 1757
MspI	CCGG	4 cut(s)	504, 520, 577, 1597
MspR9I	CCNGG	8 cut(s)	521, 578, 1415, 1481, 1501, 1609, 2032, 2096
Mva1269I	GAATGC	1 cut(s)	1361
MvaI	CCWGG	6 cut(s)	1415, 1481, 1501, 1609, 2032, 2096
MvnI	CGCG	3 cut(s)	111, 1757, 2160
NarI	GGCGCC	1 cut(s)	691
NciI	CCSGG	2 cut(s)	521, 578
NcoI	CCATGG	1 cut(s)	1797
NdeII	GATC	6 cut(s)	61, 964, 982, 1723, 1998, 2044
NlaIV	GGNNCC	4 cut(s)	63, 692, 984, 1498
NmuCI	GTSAC	3 cut(s)	715, 1519, 2068
NspI	RCATGY	4 cut(s)	793, 835, 1393, 1527
NspV	TTCGAA	1 cut(s)	147
PaeR7I	CTCGAG	1 cut(s)	1448
PagI	TCATGA	1 cut(s)	2022
PciSI	GCTCTTC	1 cut(s)	144
PcsI	WCGNNNNNNNCGW	3 cut(s)	108, 654, 2061
PctI	GAATGC	1 cut(s)	1361
PdmI	GAANNNNTTC	1 cut(s)	1203
PfeI	GAWTC	8 cut(s)	263, 337, 451, 592, 1762, 1940, 2019, 2091
PflFI	GACNNNGTC	1 cut(s)	1495
PfoI	TCCNGGA	1 cut(s)	2030
PinAI	ACCGGT	1 cut(s)	503
PkrI	GCNGC	5 cut(s)	656, 678, 1290, 1756, 2008
PleI	GAGTC	4 cut(s)	640, 1957, 2032, 2137
PluTI	GGCGCC	1 cut(s)	694
PpsI	GAGTC	4 cut(s)	640, 1957, 2032, 2137
PpuMI	RGGWCCY	2 cut(s)	563, 1497
PshBI	ATTAAT	1 cut(s)	1107
Psp5II	RGGWCCY	2 cut(s)	563, 1497
Psp6I	CCWGG	6 cut(s)	1413, 1479, 1499, 1607, 2030, 2094
PspEI	GGTNACC	1 cut(s)	958
PspGI	CCWGG	6 cut(s)	1413, 1479, 1499, 1607, 2030, 2094
PspN4I	GGNNCC	4 cut(s)	63, 692, 984, 1498
PspPI	GGNCC	4 cut(s)	563, 1463, 1497, 1503
PspPPI	RGGWCCY	2 cut(s)	563, 1497
PsrI	GAACNNNNNNTAC	2 cut(s)	863, 895
PstI	CTGCAG	1 cut(s)	678
PstNI	CAGNNNCTG	1 cut(s)	1486
PsuI	RGATCY	2 cut(s)	61, 982
PsyI	GACNNNGTC	1 cut(s)	1495
PvuII	CAGCTG	2 cut(s)	614, 955
RsaI	GTAC	5 cut(s)	71, 356, 872, 1100, 1265
RsaNI	GTAC	5 cut(s)	70, 355, 871, 1099, 1264
RseI	CAYNNNNRTG	1 cut(s)	350
SacII	CCGCGG	1 cut(s)	1758
SapI	GCTCTTC	1 cut(s)	144
SaqAI	TTAA	8 cut(s)	468, 543, 1053, 1107, 1341, 1713, 1878, 2115
SatI	GCNGC	5 cut(s)	655, 677, 1289, 1755, 2007
Sau3AI	GATC	6 cut(s)	61, 964, 982, 1723, 1998, 2044
Sau96I	GGNCC	4 cut(s)	563, 1463, 1497, 1503
ScaI	AGTACT	2 cut(s)	1100, 1265
SchI	GAGTC	4 cut(s)	641, 1957, 2032, 2138
ScrFI	CCNGG	8 cut(s)	521, 578, 1415, 1481, 1501, 1609, 2032, 2096
SduI	GDGCHC	5 cut(s)	102, 183, 383, 492, 1628
SfaNI	GCATC	4 cut(s)	537, 721, 1309, 1855
SfcI	CTRYAG	1 cut(s)	674
SfoI	GGCGCC	1 cut(s)	692
Sfr274I	CTCGAG	1 cut(s)	1448
Sfr303I	CCGCGG	1 cut(s)	1758
SfuI	TTCGAA	1 cut(s)	147
SgrBI	CCGCGG	1 cut(s)	1758
SinI	GGWCC	2 cut(s)	563, 1497
SlaI	CTCGAG	1 cut(s)	1448
SmiMI	CAYNNNNRTG	1 cut(s)	350
SmlI	CTYRAG	3 cut(s)	602, 1346, 1448
SmoI	CTYRAG	3 cut(s)	602, 1346, 1448
SpeI	ACTAGT	1 cut(s)	484
SspDI	GGCGCC	1 cut(s)	690
SspMI	CTAG	4 cut(s)	444, 485, 1454, 1515
StyD4I	CCNGG	8 cut(s)	519, 576, 1413, 1479, 1499, 1607, 2030, 2094
StyI	CCWWGG	2 cut(s)	859, 1797
TaaI	ACNGT	5 cut(s)	899, 1047, 1309, 1477, 1924
TaiI	ACGT	3 cut(s)	86, 1254, 2058
TaqII	GACCGA	1 cut(s)	1367
TatI	WGTACW	4 cut(s)	354, 870, 1098, 1263
TauI	GCSGC	2 cut(s)	1291, 1757
TfiI	GAWTC	8 cut(s)	263, 337, 451, 592, 1762, 1940, 2019, 2091
Tru1I	TTAA	8 cut(s)	468, 543, 1053, 1107, 1341, 1713, 1878, 2115
Tru9I	TTAA	8 cut(s)	468, 543, 1053, 1107, 1341, 1713, 1878, 2115
TscAI	CASTG	3 cut(s)	139, 993, 1189
TseFI	GTSAC	3 cut(s)	715, 1519, 2068
TseI	GCWGC	3 cut(s)	654, 676, 2006
Tsp45I	GTSAC	3 cut(s)	715, 1519, 2068
TspDTI	ATGAA	6 cut(s)	311, 867, 1093, 1123, 1418, 2039
TspGWI	ACGGA	4 cut(s)	872, 1220, 1556, 2123
TspRI	CASTG	3 cut(s)	139, 993, 1189
Tth111I	GACNNNGTC	1 cut(s)	1495
VpaK11BI	GGWCC	2 cut(s)	563, 1497
VspI	ATTAAT	1 cut(s)	1107
XagI	CCTNNNNNAGG	1 cut(s)	698
XapI	RAATTY	6 cut(s)	22, 188, 617, 641, 814, 1084
XceI	RCATGY	4 cut(s)	793, 835, 1393, 1527
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2038
XhoI	CTCGAG	1 cut(s)	1448
XmiI	GTMKAC	1 cut(s)	2169
XmnI	GAANNNNTTC	1 cut(s)	1203
XspI	CTAG	4 cut(s)	444, 485, 1454, 1515
ZrmI	AGTACT	2 cut(s)	1100, 1265

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.