Rroxscaffold_2G00082740

Belongs to the cullin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003908
Physical Location & Seq
Forward (+)
5495318 .. 5499292
3975 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00082740.1

Sequence Viewer

Length: 1953 bp
ATGAGTGCTCAGAAGAAGAGGAACTTCCAAATTGAGGCGTTTAAGCATCGGGTCGTGGTTGATCCGAAATACGCAGAGAAGACTTGGACGATCCTGGAGCATGCCATCAAAGAAATCTATAATCACAATGCCAGCGGGCTCAGTTTTGAAGAGCTCTATAGGAATGCTTACAATATGGTGCTGCACAAATTTGGGGAGAAACTGTACTCTGGTCTGGTGAGAACCATGACTTATCATCTGATGGAAATATCCAAATCAATTGAAGCTGCTCAGGGAGAGCTGTTTCTGGAAGAGCTTAACAGAAAATGGGCAGAGCATAACAAGGCGTTGCAGATGATTCGGGACATATTGATGTACATGGACAGAACTTTTATTCCAAGCACCCACAAAACCCCTGTTCATGAGCTTGGTCTGAACCTTTGGAGGGATGTTGTCATTCACTCGAGCAAAACCCAGTCTAGGCTTTTGGACACGCTTCTTGAGCTTGTGCTTCGAGAAAGAAATGGCGATGTCATCAACAGAGGCTTGATGAGGAATATTATAAAAATGTTAATGGATTTAGGTTCTTATGTTTACCAAGAAGACTTTGAGAAGCATTTTCTTGAAGTTTCAGCTGATTTTTACCGCTGTGAGTCTCAGGAGTTCATCGAGTCATGTGATTGTGGAAACTATTTAAAGAAGGCTGAAAGGCGCCTAAATGAGGAGATGGAGAGAGTGTCCCATTACTTAGATGCTAAAAGTGAAGCTAAGATTACCAGTGTGGTGGAAAAGGAGATGATTGAAAGTCACATGAACAGACTAGTTCATATGGAAAGCTCAGGTTTAGTTAATATGCTTGTAGATGACAAATATGAAGACTTGGGAAGAATGTATAACTTGTTTCGCAGAGTGCAGAATGGGCTTGTAATTGTAAGAGCTGTCATGACTGCTTACATCCGAGATACTGGAAAGCATCTAGTTACTGATCCAGAAAGTTTGGCATTTAACAATGACAAGACATTCCAGAATGCTTTAAACTCTTCTTTTGAGTACTTCATTAATTTGAATGCCCGGTCCCCTGAATTTATTTCTCTCTTTGTGGATGACAAGCTTCGGAAAGGTCTTAGAGGGGTTAGCGAGGAGGATGTAGAGGTTGTACTGGACAAGGTCATGATGCTTTTCCGTTACCTTCAGGAGAAAGATGTGTTTGAAAAGTATTACAAGCAACACTTGGCAAAGAGGCTTCTTTCTGGCAAAACTGTTTCTGACGATGCAGAAAGAAGTCTGATTGTAAAGCTCAAAACAGAGTGTGGATATCAGTTTACTTCCAAGTTGGAGGGTTCATGGCCTACTCAGCCAAGTGTGACATGCAACCTTCCAGCTGAAATTATGTGGGTATGTGAGAAGTTCAAGAGTTATTATCTTGGGACCCATACAGGGCGGAGATTGTCCTGGCAAACTAATATGGGAACAGCTGATTTGAAAACAACTTTTGGAAAGGGCCAGAAGCATGAGCTGAATGTTTCGACATATCAGATGTGTGTGCTGATGCTGTTCAACAATGCTGACCAGTTGACGTATAAGGAAATCGAGCAGGCTACAGAGATCCCAGCCACTGAATTGAAGAGGTGCCTGCAGTCTCTGGCCTGTGTTAAGGGGAAGAATGTTCTTCGGAAGGAGCCGATGAGCAAGGACATAGTTGAGGATGATACCTTTTTCTTCAATGACAAGTTCACAAGCAAGTTCTTTAAAGTAAAGATAGGTACCGTGGTTGCACAAAGGGAGTCTGAACCAGAAAACCTAGAAACCCGGCAGAGAGTAGAGGAAGACAGAAAGCCCCAGATTGAAGCAGCAATTGTGAGGATCATGAAGTCAAGGCGGGTACTGGATCACAATAATATAGTTGCTGAGGTCACGAAGCAGCTGCAGGCGCGATTCTTGCCCCAATCCTGTAGTAATAAAGAAGCGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 ko04340 ko04341 map04120 map04340 map04341
Pfam Domains
Protein Families
Cullin Cullin_Nedd8

Protein Analysis

650

Amino Acids

75.9

Weight (kDa)

7.59

Isoelectric Point (pI)

45.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin PF00888 29 - 322 8.7e-89 Cullin alpha solenoid domain
Cullin PF00888 327 - 440 6e-51 Cullin alpha solenoid domain
Cullin_AB PF26557 439 - 571 3.3e-49 Cullin alpha+beta domain
Cullin_Nedd8 PF10557 603 - 642 7.2e-14 Cullin protein neddylation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 542
Acc65I GGTACC 1 cut(s) 1742
AccB1I GGYRCC 3 cut(s) 690, 1608, 1742
AccII CGCG 1 cut(s) 1912
AciI CCGC 4 cut(s) 135, 625, 1420, 1858
AclWI GGATC 6 cut(s) 56, 85, 959, 1579, 1850, 1875
AcsI RAATTY 2 cut(s) 188, 1061
AcuI CTGAAG 1 cut(s) 1154
AcyI GRCGYC 1 cut(s) 691
AfaI GTAC 6 cut(s) 206, 356, 1031, 1137, 1744, 1863
AfiI CCNNNNNNNGG 5 cut(s) 34, 424, 459, 700, 1416
AhlI ACTAGT 1 cut(s) 799
AjnI CCWGG 2 cut(s) 93, 1430
AloI GAACNNNNNNTCC 2 cut(s) 358, 390
Alw21I GWGCWC 2 cut(s) 10, 156
Alw26I GTCTC 2 cut(s) 639, 1623
AlwI GGATC 6 cut(s) 56, 85, 959, 1579, 1850, 1875
AlwNI CAGNNNCTG 2 cut(s) 1595, 1621
Ama87I CYCGRG 1 cut(s) 442
AoxI GGCC 3 cut(s) 1325, 1480, 1623
ApeKI GCWGC 5 cut(s) 181, 266, 1829, 1900, 1903
ApoI RAATTY 2 cut(s) 188, 1061
AseI ATTAAT 1 cut(s) 1038
Asp718I GGTACC 1 cut(s) 1742
AspLEI GCGC 2 cut(s) 693, 1912
AspS9I GGNCC 3 cut(s) 1053, 1407, 1480
AsuC2I CCSGG 2 cut(s) 1051, 1789
AsuHPI GGTGA 1 cut(s) 229
AvaI CYCGRG 1 cut(s) 442
AvaII GGWCC 2 cut(s) 1053, 1407
BaeI ACNNNNGTAYC 2 cut(s) 1734, 1767
BanI GGYRCC 3 cut(s) 690, 1608, 1742
BanII GRGCYC 2 cut(s) 141, 156
BbsI GAAGAC 4 cut(s) 86, 588, 861, 1812
Bbv12I GWGCWC 2 cut(s) 10, 156
BbvCI CCTCAGC 1 cut(s) 1887
BbvI GCAGC 5 cut(s) 168, 253, 1841, 1890, 1912
BccI CCATC 3 cut(s) 113, 235, 700
BcgI CGANNNNNNTGC 4 cut(s) 320, 354, 1885, 1919
BciT130I CCWGG 2 cut(s) 95, 1432
BcnI CCSGG 2 cut(s) 1051, 1789
BcoDI GTCTC 2 cut(s) 639, 1623
BcuI ACTAGT 1 cut(s) 799
BfaI CTAG 4 cut(s) 459, 800, 956, 1781
BfmI CTRYAG 5 cut(s) 157, 1578, 1613, 1904, 1930
BfoI RGCGCY 1 cut(s) 694
BisI GCNGC 5 cut(s) 182, 267, 1830, 1901, 1904
BlsI GCNGC 5 cut(s) 183, 268, 1831, 1902, 1905
BmcAI AGTACT 1 cut(s) 1031
Bme1390I CCNGG 4 cut(s) 95, 1051, 1432, 1789
Bme18I GGWCC 2 cut(s) 1053, 1407
BmeT110I CYCGRG 1 cut(s) 442
BmgT120I GGNCC 3 cut(s) 1053, 1407, 1480
BmiI GGNNCC 7 cut(s) 692, 1055, 1408, 1409, 1610, 1659, 1744
BmrFI CCNGG 4 cut(s) 95, 1051, 1432, 1789
BmrI ACTGGG 1 cut(s) 448
BmsI GCATC 6 cut(s) 55, 721, 961, 1143, 1240, 1518
BmuI ACTGGG 1 cut(s) 448
BpiI GAAGAC 4 cut(s) 86, 588, 861, 1812
BpmI CTGGAG 1 cut(s) 116
Bpu10I CCTNAGC 3 cut(s) 270, 817, 1887
BpuEI CTTGAG 1 cut(s) 500
BpuMI CCSGG 2 cut(s) 1051, 1789
BsaHI GRCGYC 1 cut(s) 691
BsaJI CCNNGG 1 cut(s) 1746
BsaXI ACNNNNNCTCC 4 cut(s) 188, 218, 701, 731
Bsc4I CCNNNNNNNGG 5 cut(s) 34, 424, 459, 700, 1416
Bse1I ACTGG 6 cut(s) 454, 756, 949, 1143, 1549, 1869
BseBI CCWGG 2 cut(s) 95, 1432
BseDI CCNNGG 1 cut(s) 1746
BseGI GGATG 5 cut(s) 433, 933, 1087, 1129, 1690
BseLI CCNNNNNNNGG 5 cut(s) 34, 424, 459, 700, 1416
BseMII CTCAG 7 cut(s) 23, 154, 284, 650, 831, 1346, 1878
BseNI ACTGG 6 cut(s) 454, 756, 949, 1143, 1549, 1869
BseRI GAGGAG 2 cut(s) 716, 1133
BseXI GCAGC 5 cut(s) 168, 253, 1841, 1890, 1912
BseYI CCCAGC 1 cut(s) 1588
BsgI GTGCAG 2 cut(s) 167, 911
Bsh1236I CGCG 1 cut(s) 1912
BshFI GGCC 3 cut(s) 1327, 1482, 1625
BshNI GGYRCC 3 cut(s) 690, 1608, 1742
BsiHKAI GWGCWC 2 cut(s) 10, 156
BsiHKCI CYCGRG 1 cut(s) 442
BsiSI CCGG 2 cut(s) 1051, 1789
BslFI GGGAC 4 cut(s) 356, 703, 1039, 1420
BslI CCNNNNNNNGG 5 cut(s) 34, 424, 459, 700, 1416
BsmAI GTCTC 2 cut(s) 639, 1623
BsmFI GGGAC 4 cut(s) 356, 703, 1039, 1420
BsmI GAATGC 3 cut(s) 169, 1012, 1051
BsnI GGCC 3 cut(s) 1327, 1482, 1625
BsoBI CYCGRG 1 cut(s) 442
Bsp1286I GDGCHC 3 cut(s) 10, 141, 156
Bsp1407I TGTACA 1 cut(s) 354
Bsp143I GATC 6 cut(s) 61, 90, 964, 1584, 1842, 1867
BspACI CCGC 4 cut(s) 135, 625, 1420, 1858
BspANI GGCC 3 cut(s) 1327, 1482, 1625
BspCNI CTCAG 7 cut(s) 22, 153, 283, 649, 830, 1345, 1879
BspFNI CGCG 1 cut(s) 1912
BspHI TCATGA 4 cut(s) 400, 921, 1149, 1845
BspLI GGNNCC 7 cut(s) 692, 1055, 1408, 1409, 1610, 1659, 1744
BspMAI CTGCAG 2 cut(s) 1617, 1908
BspPI GGATC 6 cut(s) 56, 85, 959, 1579, 1850, 1875
BspQI GCTCTTC 2 cut(s) 144, 285
BspT107I GGYRCC 3 cut(s) 690, 1608, 1742
BsrGI TGTACA 1 cut(s) 354
BsrI ACTGG 6 cut(s) 454, 756, 949, 1143, 1549, 1869
BssECI CCNNGG 1 cut(s) 1746
BssMI GATC 6 cut(s) 61, 90, 964, 1584, 1842, 1867
BssNI GRCGYC 1 cut(s) 691
Bst2UI CCWGG 2 cut(s) 95, 1432
Bst4CI ACNGT 3 cut(s) 204, 1240, 1747
Bst6I CTCTTC 5 cut(s) 11, 144, 285, 1024, 1598
BstACI GRCGYC 1 cut(s) 691
BstAUI TGTACA 1 cut(s) 354
BstC8I GCNNGC 6 cut(s) 102, 133, 137, 1575, 1613, 1908
BstDSI CCRYGG 1 cut(s) 1746
BstENI CCTNNNNNAGG 1 cut(s) 698
BstF5I GGATG 5 cut(s) 433, 933, 1087, 1129, 1690
BstFNI CGCG 1 cut(s) 1912
BstH2I RGCGCY 1 cut(s) 694
BstHHI GCGC 2 cut(s) 693, 1912
BstKTI GATC 6 cut(s) 64, 93, 967, 1587, 1845, 1870
BstMAI GTCTC 2 cut(s) 639, 1623
BstMBI GATC 6 cut(s) 61, 90, 964, 1584, 1842, 1867
BstMWI GCNNNNNNNGC 5 cut(s) 481, 898, 1333, 1909, 1918
BstNI CCWGG 2 cut(s) 95, 1432
BstNSI RCATGY 2 cut(s) 104, 1350
BstSCI CCNGG 4 cut(s) 93, 1049, 1430, 1787
BstSFI CTRYAG 5 cut(s) 157, 1578, 1613, 1904, 1930
BstUI CGCG 1 cut(s) 1912
BstV1I GCAGC 5 cut(s) 168, 253, 1841, 1890, 1912
BstV2I GAAGAC 4 cut(s) 86, 588, 861, 1812
BstX2I RGATCY 1 cut(s) 1584
BstXI CCANNNNNNTGG 1 cut(s) 763
BstYI RGATCY 1 cut(s) 1584
BsuRI GGCC 3 cut(s) 1327, 1482, 1625
BtgI CCRYGG 1 cut(s) 1746
BtgZI GCGATG 1 cut(s) 522
BtsCI GGATG 5 cut(s) 433, 933, 1087, 1129, 1690
BtsIMutI CAGTG 2 cut(s) 763, 1593
Cac8I GCNNGC 6 cut(s) 102, 133, 137, 1575, 1613, 1908
CaiI CAGNNNCTG 2 cut(s) 1595, 1621
CciI TCATGA 4 cut(s) 400, 921, 1149, 1845
CfoI GCGC 2 cut(s) 693, 1912
Cfr13I GGNCC 3 cut(s) 1053, 1407, 1480
Csp6I GTAC 6 cut(s) 205, 355, 1030, 1136, 1743, 1862
CviQI GTAC 6 cut(s) 205, 355, 1030, 1136, 1743, 1862
DinI GGCGCC 1 cut(s) 692
DpnI GATC 6 cut(s) 63, 92, 966, 1586, 1844, 1869
DpnII GATC 6 cut(s) 61, 90, 964, 1584, 1842, 1867
DraI TTTAAA 3 cut(s) 675, 1014, 1729
Eam1104I CTCTTC 5 cut(s) 11, 144, 285, 1024, 1598
EarI CTCTTC 5 cut(s) 11, 144, 285, 1024, 1598
EciI GGCGGA 1 cut(s) 1435
Ecl136II GAGCTC 1 cut(s) 154
Eco24I GRGCYC 2 cut(s) 141, 156
Eco32I GATATC 1 cut(s) 1295
Eco47I GGWCC 2 cut(s) 1053, 1407
Eco53kI GAGCTC 1 cut(s) 154
Eco57I CTGAAG 1 cut(s) 1154
Eco88I CYCGRG 1 cut(s) 442
EcoICRI GAGCTC 1 cut(s) 154
EcoNI CCTNNNNNAGG 1 cut(s) 698
EcoO109I RGGNCCY 1 cut(s) 1407
EcoRII CCWGG 2 cut(s) 93, 1430
EcoRV GATATC 1 cut(s) 1295
EcoT38I GRGCYC 2 cut(s) 141, 156
EgeI GGCGCC 1 cut(s) 692
EheI GGCGCC 1 cut(s) 692
FalI AAGNNNNNCTT 4 cut(s) 8, 40, 1193, 1225
FaqI GGGAC 4 cut(s) 356, 703, 1039, 1420
FauI CCCGC 2 cut(s) 128, 1851
FauNDI CATATG 1 cut(s) 807
Fnu4HI GCNGC 5 cut(s) 182, 267, 1830, 1901, 1904
FokI GGATG 5 cut(s) 440, 920, 1094, 1136, 1697
FriOI GRGCYC 2 cut(s) 141, 156
Fsp4HI GCNGC 5 cut(s) 182, 267, 1830, 1901, 1904
FspBI CTAG 4 cut(s) 459, 800, 956, 1781
GlaI GCGC 2 cut(s) 692, 1911
GluI GCNGC 5 cut(s) 182, 267, 1830, 1901, 1904
GsaI CCCAGC 1 cut(s) 1592
GsuI CTGGAG 1 cut(s) 116
HaeII RGCGCY 1 cut(s) 694
HaeIII GGCC 3 cut(s) 1327, 1482, 1625
HapII CCGG 2 cut(s) 1051, 1789
HhaI GCGC 2 cut(s) 693, 1912
Hin1I GRCGYC 1 cut(s) 691
Hin6I GCGC 2 cut(s) 691, 1910
HinP1I GCGC 2 cut(s) 691, 1910
HincII GTYRAC 1 cut(s) 1554
HindII GTYRAC 1 cut(s) 1554
HindIII AAGCTT 1 cut(s) 1088
HinfI GANTC 5 cut(s) 337, 632, 650, 1763, 1914
HpaII CCGG 2 cut(s) 1051, 1789
HphI GGTGA 1 cut(s) 229
Hpy166II GTNNAC 4 cut(s) 574, 1302, 1554, 1713
Hpy8I GTNNAC 4 cut(s) 574, 1302, 1554, 1713
HpyAV CCTTC 4 cut(s) 673, 1178, 1364, 1648
HpyCH4III ACNGT 3 cut(s) 204, 1240, 1747
HpyCH4IV ACGT 1 cut(s) 1556
HpyCH4V TGCA 8 cut(s) 184, 331, 892, 1253, 1350, 1615, 1754, 1906
HpyF10VI GCNNNNNNNGC 5 cut(s) 481, 898, 1333, 1909, 1918
HpySE526I ACGT 1 cut(s) 1556
Hsp92I GRCGYC 1 cut(s) 691
HspAI GCGC 2 cut(s) 691, 1910
KasI GGCGCC 1 cut(s) 690
KflI GGGWCCC 1 cut(s) 1407
KpnI GGTACC 1 cut(s) 1746
Kzo9I GATC 6 cut(s) 61, 90, 964, 1584, 1842, 1867
LguI GCTCTTC 2 cut(s) 144, 285
LmnI GCTCC 2 cut(s) 97, 1657
Lsp1109I GCAGC 5 cut(s) 168, 253, 1841, 1890, 1912
LweI GCATC 6 cut(s) 55, 721, 961, 1143, 1240, 1518
MaeI CTAG 4 cut(s) 459, 800, 956, 1781
MaeII ACGT 1 cut(s) 1556
MaeIII GTNAC 5 cut(s) 785, 958, 1163, 1342, 1891
MalI GATC 6 cut(s) 63, 92, 966, 1586, 1844, 1869
MboI GATC 6 cut(s) 61, 90, 964, 1584, 1842, 1867
MfeI CAATTG 2 cut(s) 258, 1833
MflI RGATCY 1 cut(s) 1584
MhlI GDGCHC 3 cut(s) 10, 141, 156
MluCI AATT 9 cut(s) 30, 188, 258, 906, 1039, 1061, 1365, 1598, 1833
Mly113I GGCGCC 1 cut(s) 691
MlyI GAGTC 3 cut(s) 641, 659, 1772
MmeI TCCRAC 1 cut(s) 1293
MslI CAYNNNNRTG 1 cut(s) 350
MspA1I CMGCKG 6 cut(s) 135, 614, 627, 1361, 1454, 1903
MspI CCGG 2 cut(s) 1051, 1789
MspR9I CCNGG 4 cut(s) 95, 1051, 1432, 1789
MunI CAATTG 2 cut(s) 258, 1833
Mva1269I GAATGC 3 cut(s) 169, 1012, 1051
MvaI CCWGG 2 cut(s) 95, 1432
MvnI CGCG 1 cut(s) 1912
MwoI GCNNNNNNNGC 5 cut(s) 481, 898, 1333, 1909, 1918
NarI GGCGCC 1 cut(s) 691
NciI CCSGG 2 cut(s) 1051, 1789
NdeI CATATG 1 cut(s) 807
NdeII GATC 6 cut(s) 61, 90, 964, 1584, 1842, 1867
NlaIV GGNNCC 7 cut(s) 692, 1055, 1408, 1409, 1610, 1659, 1744
NmuCI GTSAC 3 cut(s) 785, 1342, 1891
NspI RCATGY 2 cut(s) 104, 1350
PaeI GCATGC 1 cut(s) 104
PaeR7I CTCGAG 1 cut(s) 442
PagI TCATGA 4 cut(s) 400, 921, 1149, 1845
PciSI GCTCTTC 2 cut(s) 144, 285
PctI GAATGC 3 cut(s) 169, 1012, 1051
PfeI GAWTC 2 cut(s) 337, 1914
PflFI GACNNNGTC 1 cut(s) 1145
PfoI TCCNGGA 1 cut(s) 93
PkrI GCNGC 5 cut(s) 183, 268, 1831, 1902, 1905
PleI GAGTC 3 cut(s) 640, 658, 1771
PluTI GGCGCC 1 cut(s) 694
PpsI GAGTC 3 cut(s) 640, 658, 1771
PpuMI RGGWCCY 1 cut(s) 1407
PshBI ATTAAT 1 cut(s) 1038
PsiI TTATAA 1 cut(s) 542
Psp124BI GAGCTC 1 cut(s) 156
Psp5II RGGWCCY 1 cut(s) 1407
Psp6I CCWGG 2 cut(s) 93, 1430
PspFI CCCAGC 1 cut(s) 1588
PspGI CCWGG 2 cut(s) 93, 1430
PspN4I GGNNCC 7 cut(s) 692, 1055, 1408, 1409, 1610, 1659, 1744
PspPI GGNCC 3 cut(s) 1053, 1407, 1480
PspPPI RGGWCCY 1 cut(s) 1407
PspXI VCTCGAGB 1 cut(s) 442
PstI CTGCAG 2 cut(s) 1617, 1908
PstNI CAGNNNCTG 2 cut(s) 1595, 1621
PsuI RGATCY 1 cut(s) 1584
PsyI GACNNNGTC 1 cut(s) 1145
PvuII CAGCTG 4 cut(s) 614, 1361, 1454, 1903
RsaI GTAC 6 cut(s) 206, 356, 1031, 1137, 1744, 1863
RsaNI GTAC 6 cut(s) 205, 355, 1030, 1136, 1743, 1862
RseI CAYNNNNRTG 1 cut(s) 350
SacI GAGCTC 1 cut(s) 156
SapI GCTCTTC 2 cut(s) 144, 285
SatI GCNGC 5 cut(s) 182, 267, 1830, 1901, 1904
Sau3AI GATC 6 cut(s) 61, 90, 964, 1584, 1842, 1867
Sau96I GGNCC 3 cut(s) 1053, 1407, 1480
ScaI AGTACT 1 cut(s) 1031
SchI GAGTC 3 cut(s) 641, 659, 1772
ScrFI CCNGG 4 cut(s) 95, 1051, 1432, 1789
SduI GDGCHC 3 cut(s) 10, 141, 156
SfaNI GCATC 6 cut(s) 55, 721, 961, 1143, 1240, 1518
SfcI CTRYAG 5 cut(s) 157, 1578, 1613, 1904, 1930
SfoI GGCGCC 1 cut(s) 692
Sfr274I CTCGAG 1 cut(s) 442
SinI GGWCC 2 cut(s) 1053, 1407
SlaI CTCGAG 1 cut(s) 442
SmiMI CAYNNNNRTG 1 cut(s) 350
SmlI CTYRAG 2 cut(s) 442, 479
SmoI CTYRAG 2 cut(s) 442, 479
SpeI ACTAGT 1 cut(s) 799
SphI GCATGC 1 cut(s) 104
Sse9I AATT 9 cut(s) 30, 188, 258, 906, 1039, 1061, 1365, 1598, 1833
SsiI CCGC 4 cut(s) 135, 625, 1420, 1858
SspDI GGCGCC 1 cut(s) 690
SspI AATATT 1 cut(s) 538
SspMI CTAG 4 cut(s) 459, 800, 956, 1781
SstI GAGCTC 1 cut(s) 156
StyD4I CCNGG 4 cut(s) 93, 1049, 1430, 1787
TaaI ACNGT 3 cut(s) 204, 1240, 1747
TaiI ACGT 1 cut(s) 1559
TaqI TCGA 5 cut(s) 443, 493, 648, 1505, 1569
TasI AATT 9 cut(s) 30, 188, 258, 906, 1039, 1061, 1365, 1598, 1833
TatI WGTACW 4 cut(s) 204, 354, 1029, 1135
TfiI GAWTC 2 cut(s) 337, 1914
TscAI CASTG 2 cut(s) 763, 1600
TseFI GTSAC 3 cut(s) 785, 1342, 1891
TseI GCWGC 5 cut(s) 181, 266, 1829, 1900, 1903
Tsp45I GTSAC 3 cut(s) 785, 1342, 1891
TspDTI ATGAA 8 cut(s) 389, 634, 794, 806, 867, 1024, 1311, 1862
TspGWI ACGGA 1 cut(s) 1151
TspRI CASTG 2 cut(s) 763, 1600
Tth111I GACNNNGTC 1 cut(s) 1145
VpaK11BI GGWCC 2 cut(s) 1053, 1407
VspI ATTAAT 1 cut(s) 1038
XagI CCTNNNNNAGG 1 cut(s) 698
XapI RAATTY 2 cut(s) 188, 1061
XceI RCATGY 2 cut(s) 104, 1350
XhoI CTCGAG 1 cut(s) 442
XspI CTAG 4 cut(s) 459, 800, 956, 1781
ZrmI AGTACT 1 cut(s) 1031
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.