MD09G1066900.v1.1

Protein CHLORORESPIRATORY REDUCTION 7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0014482
Physical Location & Seq
Reverse (-)
4550168 .. 4551468
1301 bp
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UTR
Exon/CDS
Intron
MD09G1066900.v1.1.491

Sequence Viewer

Length: 462 bp
ATGGTTTCAATGGAAGGAGCTTTGAAGAAACAGTTGTTCCATGGTGGATTTCTGACCCTTAACTGCAAAGAAGGTACAAAGTGCCAGATTCGAGCTCCGTTGATGCAGTTAGTGGCAACAGGAAAAGATGATATTAGTATTCATCTTAATCTGTTTAAAACAAGGAGTGTGCAAAGAGACGCAGTCAAGGTTTGTGCAGTGAGGAGGAGAAGGATATACCATAATAGTGAAACTTATGTGTTACTGGAGCCGGGAGAGGATGAGAAGTTTGTTTCAGAGGATGAGCTGAGGGACAAGTTAAAAGGTTGGCTGGAGAACTGGCCAGCCAAGAAACTTCCTCCTGACCTTGCAAGATTCGAAAGCATCGACGATGCCGTTTCTTATCTAGTGAGGTCTGTTTGTGAACTTGAAATTCATGGAGATGTAGGTTCAGTCCAGTGGTACGAAGTTCGAATGGAGTGA

Protein Analysis

154

Amino Acids

17.61

Weight (kDa)

6.44

Isoelectric Point (pI)

37.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRR7 PF12095 72 - 153 1.7e-28 Protein CHLORORESPIRATORY REDUCTION 7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014482)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G39210
fragaria_vesca FvH4_6g46840
malus_domestica MD09G1066900.v1.1
prunus_persica Prupe.3G254800_v2.0.a1
pyrus_communis pycom111g05620 pycom17g05950
rosa_chinensis RchiOBHm_Chr2g0165831
rosa_laevigata RLG00000021560
rosa_multiflora Rmu_sc0003227.1_g000001 Rmu_sc0008709.1_g000001
rosa_roxburghii Rroxscaffold_2G00085460
rosa_rugosa Rorug02G0518200
rosa_samantha Rh2AG584000 Rh2BG595900 Rh2CG566900 Rh2DG605700
rosa_wichuraiana Rw2G048690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 320
AcsI RAATTY 1 cut(s) 411
AfaI GTAC 2 cut(s) 76, 443
AgsI TTSAA 3 cut(s) 9, 25, 410
AluBI AGCT 3 cut(s) 20, 95, 286
AluI AGCT 3 cut(s) 20, 95, 286
Alw21I GWGCWC 1 cut(s) 97
Alw26I GTCTC 1 cut(s) 171
AoxI GGCC 1 cut(s) 320
ApoI RAATTY 1 cut(s) 411
AsuC2I CCSGG 1 cut(s) 252
AsuII TTCGAA 2 cut(s) 357, 451
BalI TGGCCA 1 cut(s) 322
BanII GRGCYC 1 cut(s) 97
Bbv12I GWGCWC 1 cut(s) 97
BbvCI CCTCAGC 1 cut(s) 287
BceAI ACGGC 1 cut(s) 359
BcnI CCSGG 1 cut(s) 252
BcoDI GTCTC 1 cut(s) 171
BfaI CTAG 1 cut(s) 386
Bme1390I CCNGG 1 cut(s) 252
BmiI GGNNCC 1 cut(s) 249
BmrFI CCNGG 1 cut(s) 252
BmsI GCATC 3 cut(s) 93, 361, 372
BpmI CTGGAG 2 cut(s) 266, 332
Bpu10I CCTNAGC 1 cut(s) 287
Bpu14I TTCGAA 2 cut(s) 357, 451
BpuMI CCSGG 1 cut(s) 252
BsaJI CCNNGG 1 cut(s) 40
Bse1I ACTGG 3 cut(s) 249, 323, 436
BseDI CCNNGG 1 cut(s) 40
BseGI GGATG 2 cut(s) 265, 286
BseMII CTCAG 1 cut(s) 278
BseNI ACTGG 3 cut(s) 249, 323, 436
BseRI GAGGAG 2 cut(s) 217, 220
BsgI GTGCAG 1 cut(s) 216
BshFI GGCC 1 cut(s) 322
BsiHKAI GWGCWC 1 cut(s) 97
BsiSI CCGG 1 cut(s) 251
BslFI GGGAC 1 cut(s) 305
BsmAI GTCTC 1 cut(s) 171
BsmBI CGTCTC 1 cut(s) 171
BsmFI GGGAC 1 cut(s) 305
BsnI GGCC 1 cut(s) 322
Bsp119I TTCGAA 2 cut(s) 357, 451
Bsp1286I GDGCHC 1 cut(s) 97
Bsp19I CCATGG 1 cut(s) 40
BspANI GGCC 1 cut(s) 322
BspCNI CTCAG 1 cut(s) 279
BspLI GGNNCC 1 cut(s) 249
BspT104I TTCGAA 2 cut(s) 357, 451
BsrI ACTGG 3 cut(s) 249, 323, 436
BssECI CCNNGG 1 cut(s) 40
BssT1I CCWWGG 1 cut(s) 40
Bst4CI ACNGT 1 cut(s) 33
BstBI TTCGAA 2 cut(s) 357, 451
BstC8I GCNNGC 1 cut(s) 324
BstDEI CTNAG 1 cut(s) 287
BstDSI CCRYGG 1 cut(s) 40
BstF5I GGATG 2 cut(s) 265, 286
BstMAI GTCTC 1 cut(s) 171
BstSCI CCNGG 1 cut(s) 250
BsuRI GGCC 1 cut(s) 322
BtgI CCRYGG 1 cut(s) 40
BtsCI GGATG 2 cut(s) 265, 286
BtsI GCAGTG 1 cut(s) 204
BtsIMutI CAGTG 2 cut(s) 204, 443
Cac8I GCNNGC 1 cut(s) 324
CseI GACGC 1 cut(s) 188
Csp6I GTAC 2 cut(s) 75, 442
CviAII CATG 2 cut(s) 41, 416
CviJI RGCY 7 cut(s) 20, 95, 250, 286, 310, 322, 326
CviKI_1 RGCY 7 cut(s) 20, 95, 250, 286, 310, 322, 326
CviQI GTAC 2 cut(s) 75, 442
DdeI CTNAG 1 cut(s) 287
DraI TTTAAA 1 cut(s) 157
EaeI YGGCCR 1 cut(s) 320
Ecl136II GAGCTC 1 cut(s) 95
Eco130I CCWWGG 1 cut(s) 40
Eco24I GRGCYC 1 cut(s) 97
Eco53kI GAGCTC 1 cut(s) 95
EcoICRI GAGCTC 1 cut(s) 95
EcoT14I CCWWGG 1 cut(s) 40
EcoT38I GRGCYC 1 cut(s) 97
ErhI CCWWGG 1 cut(s) 40
Esp3I CGTCTC 1 cut(s) 171
FaeI CATG 2 cut(s) 44, 419
FaiI YATR 5 cut(s) 42, 217, 222, 237, 417
FaqI GGGAC 1 cut(s) 305
FatI CATG 2 cut(s) 40, 415
FokI GGATG 2 cut(s) 272, 293
FriOI GRGCYC 1 cut(s) 97
FspBI CTAG 1 cut(s) 386
GsuI CTGGAG 2 cut(s) 266, 332
HaeIII GGCC 1 cut(s) 322
HapII CCGG 1 cut(s) 251
HgaI GACGC 1 cut(s) 188
Hin1II CATG 2 cut(s) 44, 419
HinfI GANTC 2 cut(s) 88, 354
HpaII CCGG 1 cut(s) 251
Hpy166II GTNNAC 1 cut(s) 404
Hpy188I TCNGA 2 cut(s) 54, 277
Hpy188III TCNNGA 1 cut(s) 341
Hpy8I GTNNAC 1 cut(s) 404
Hpy99I CGWCG 1 cut(s) 371
HpyAV CCTTC 3 cut(s) 8, 65, 204
HpyCH4III ACNGT 1 cut(s) 33
HpyCH4V TGCA 5 cut(s) 66, 106, 172, 197, 350
HpyF3I CTNAG 1 cut(s) 287
Hsp92II CATG 2 cut(s) 44, 419
LmnI GCTCC 3 cut(s) 17, 100, 247
LpnPI CCDG 9 cut(s) 98, 105, 230, 264, 296, 304, 336, 354, 449
LweI GCATC 3 cut(s) 93, 361, 372
MaeI CTAG 1 cut(s) 386
MaeIII GTNAC 1 cut(s) 240
MboII GAAGA 1 cut(s) 37
MhlI GDGCHC 1 cut(s) 97
MlsI TGGCCA 1 cut(s) 322
MluCI AATT 1 cut(s) 411
MluNI TGGCCA 1 cut(s) 322
MnlI CCTC 7 cut(s) 195, 198, 250, 271, 282, 348, 384
Mox20I TGGCCA 1 cut(s) 322
MscI TGGCCA 1 cut(s) 322
MseI TTAA 4 cut(s) 60, 147, 156, 299
MslI CAYNNNNRTG 2 cut(s) 225, 420
Msp20I TGGCCA 1 cut(s) 322
MspI CCGG 1 cut(s) 251
MspR9I CCNGG 1 cut(s) 252
NciI CCSGG 1 cut(s) 252
NcoI CCATGG 1 cut(s) 40
NlaIII CATG 2 cut(s) 44, 419
NlaIV GGNNCC 1 cut(s) 249
NspV TTCGAA 2 cut(s) 357, 451
PcsI WCGNNNNNNNCGW 2 cut(s) 363, 372
PfeI GAWTC 2 cut(s) 88, 354
PflFI GACNNNGTC 1 cut(s) 182
Psp124BI GAGCTC 1 cut(s) 97
PspN4I GGNNCC 1 cut(s) 249
PsyI GACNNNGTC 1 cut(s) 182
RsaI GTAC 2 cut(s) 76, 443
RsaNI GTAC 2 cut(s) 75, 442
RseI CAYNNNNRTG 2 cut(s) 225, 420
SacI GAGCTC 1 cut(s) 97
SaqAI TTAA 4 cut(s) 60, 147, 156, 299
ScrFI CCNGG 1 cut(s) 252
SduI GDGCHC 1 cut(s) 97
SetI ASST 9 cut(s) 22, 76, 97, 192, 288, 307, 348, 395, 430
SfaNI GCATC 3 cut(s) 93, 361, 372
SfuI TTCGAA 2 cut(s) 357, 451
SmiMI CAYNNNNRTG 2 cut(s) 225, 420
Sse9I AATT 1 cut(s) 411
SspMI CTAG 1 cut(s) 386
SstI GAGCTC 1 cut(s) 97
StyD4I CCNGG 1 cut(s) 250
StyI CCWWGG 1 cut(s) 40
TaaI ACNGT 1 cut(s) 33
TaqI TCGA 4 cut(s) 91, 357, 366, 451
TasI AATT 1 cut(s) 411
TfiI GAWTC 2 cut(s) 88, 354
Tru1I TTAA 4 cut(s) 60, 147, 156, 299
Tru9I TTAA 4 cut(s) 60, 147, 156, 299
TscAI CASTG 2 cut(s) 204, 443
TspDTI ATGAA 2 cut(s) 131, 404
TspGWI ACGGA 1 cut(s) 87
TspRI CASTG 2 cut(s) 204, 443
Tth111I GACNNNGTC 1 cut(s) 182
XapI RAATTY 1 cut(s) 411
XspI CTAG 1 cut(s) 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.