Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0010808

Physical Location & Seq

Genomic Coordinates Forward (+)

4557201 .. 4562453

Gene Structure 5253 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1067100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3432 bp

ATGGGTTTTTCTCCGTCTTCCTCTTCGGTCATCAAAGGACATTTTTTTCGCAGACCCACTTCTCCAGTGTTTGTTCTGAAGCTTGTTTTGCTCTTGTGGGTGTTCTGTAGCTCCCAGAACTGTGCTGTTCTCGCTGATACTGACGCATCGGTGCTTCTTGAGCTAAAAAATTCTGTCTTGGATCCTTCAGGCTTGCTTTCTGGCTGGAACGGTCGGCTCAGTTCTGGTCATTGCTCATGGACCGGCGTCTTCTGCAACTCAAATTCTCGAGTGGTTTCGCTCAACCTTACTGGGAATGGCGGAGAAACCGAAGGTAGATCGAAAACTATTGCGTGCTCAGATTTTTCTCAGTTGCCGCTTTATGGATTCGGAATCAGAAGGAGCTGTGAGGGTAGTAGAGGAAGATTGGCCGGGAAGCTCCCCTCTGTAATTGGTAAGCTCACTGAGCTTAGGGTCTTGTCCCTCCCCTTCAATGGTTTTTATGGTGAAATTCCTAGTGAAGTTTTGGGCTTGGAAAACTTGGAGGTTCTTGATCTTGAGGGAAACTCGGTAACTGGGTCGCTGCCCTTTCAGCTTAATCCAAATTTGAGGGTTCTTAATCTGGGGTTTAATATGATTAAAGGTGAGATACCGACCTCGTGGTCAAATTATATGAGCCTAGAGATCTTGAGTTTAGCTGGGAATCTCGTAAATGGAACCGTTCCAGGTTTCATTGGTAGGTTAAAGGCAGTGTACTTGTCATACAATTCACTTAGTGGAGATGTTCCCAGTGAGATTGGAGCAAATTGTGGGAAGCTTGAGCATCTTGATTTGTCGGGTAATTTCTTGGTTCATAACATTCCAAGCAGTTTGGGAAACTGCAGCCAGTTGAGGACGTTGTTGCTGTATTCAAATATGTTTGAAGAGGGTATTCCAGCTGAACTCGGACGGCTTCAAGCTCTGGAAGTGTTGGATGTGTCAAGGAATACCCTGAGTAGTTCATTACCACGTGAGCTGGGGAATTGTTCTCAGTTGTCTGTCCTTGTGCTGTCATCTATGTTTAATCCACTTCCACGAGGTAACGATACCGTAGTGGACTCTTTGCTGGAGCAGTCGAATGCTATGAATGACGACTTCAATTACTTTCAAGGTACAATGCCTGTAGAAATTACAACCCTTCCAAAGCTAAGGATCTTGTGGGCACCAAGGGCAAGTATTGAGGGCACTTTCCCAAGCAATTGGGGTGGTTGTGAGTACTTAGAGATGATCAATTTGGCTCAGAACTTTTTGACTGGGGAATTTACTAGTGGACTTAGTCGCTGCAGGAAGCTTCAGTTTCTTGACGTGAGCTCTAACAGGCTTAGTGGGGAGCTTGTTCAGGATCTTCATGTTCCTTGTATGGTTATGTTTGATATCAGCAGAAACATGTTGTCAGGTTCAATACCTGAATATTTCAACAGAACTTGTGCTTCTCCTTTTGGAGATTTTTCTTTCAAGTCTGAGGATCCATCCTCAACCTATGTTGCATTTTTCGCTTCTAAATCCCAAGTTGGAAATCCATTGCAAATGCACGGGGAGGGTGACAGTCTTATAGTAGTACACAACTTCGCGCACAATAATTTTACTGGCGCTTTGCAGACGCTGCCTATTGCACCTGAAAGACTAGGGAAACAAACTTTTTATGCATTCTTTGCTGGAGAAAACAAGATTTCTGGAGAATTTCCAGGTAAATTATTTGGCAAGTGTGAGGGATTGGAGTGGCTGATTGTTAATGTAAGCCACAACAAGTTAGAAGGTCAGATTCCAGCTGAAGTAGGCACTATGTGCAAATCTCTTAAAGGTTTAGATGCATCTCGGAATCAGATTATTGGTCCAATTCCCTCTACCATCGGGAAACTGTCTCTTGTCGCTCTTAATTTGAGTTGGAACATGTTGAGGGGTGAAATCCCGACCAGTCTTGGCCAGATAAGGAATCTGATGTATCTTTCCTTATCTGGCAATCTCCTTACAGGTGTCATTCCGTCTAGTTTGGGGCAGCTGACCTCTTTAGAAGTGCTTGAACTTTCTTCAAACCATCTGACTGGTGAGATACCGAAAGATCTTGTGAATCTGCGAAACCTGACTGTTCTTCTGCTCGATAGGAATAATATTTCTGGTCAGATTCCATCTGGATTGGCAAATGTGACCACACTGTCATCTTTCAATGTGTCTTTCAATAACATCACAGGTTCACTGCCTGCGAATAATAACCTGATGAAATGCAATGCTGCTATTGGAAACCCAAATCTAGACTCTTGCCCCATGTTTTCTCTGGCACAGCCAGCTTCAGATTCTCAAGGACGAGTTGGTGATTCACAACCTTTTGCTGCTTCACCAGTGGGAGGTCCAGCTCAGAAGACTGGGAACGGTTTCAATTCAATTGAAATAGCATCCATTACATCTGCATCGGCCATAGTTTTGGTGCTTATTGCTCTTGTTATTCTGTTCTTGTATACACGGAAATGGAATCCAAAGTCTGGAACTCAAGGTTCTACTAGGAAGGAAGTTACAATTTTCACAAACATTGGGGTTCCATTGACCTTTGAGAATGTTGTGGGAGCCACAGGAGGTTTCAATGCTAGTAACTGTATTGGGAATGGAGGTTTTGGAGCTACCTACAAGGCAGAGATATCACCTGGAATCGTGGTGGCAATTAAACGACTTTCAGTTGGAAGGTTCCAAGGAGTTCAACAGTTTCATGCAGAAATCAAAACCCTTGGAAGGCTTCGCCATCCAAATCTTGTCACTTTGCTTGGTTATCATGCCAGTGATACAGAGATGTTTCTAATTTACAACTACTTGGCGGGTGGTAATTTGGAAAAGTTTATTCAGGAAAGGTCAACAAGGGCTGTGGATTGGAGGGTACTTCACAAGATTGCTTTGGACATAGCCCGTGCACTTGCCTACCTACATGATCAGTGCGTACCACGTGTCCTTCATCGCGACGTCAAGCCGAGTAATATTTTGTTGGATGATGATTTTAATGCGTATCTCTCAGACTTTGGTTTGGCCAGACTTCTGGGAACTTCGGAGACCCATGCTACTACTGGCGTTGCTGGAACTTTTGGATATGTTGCCCCAGAATATGCAATGACTTGCCGTGTTTCAGATAAGGCTGATGTATATAGTTATGGCGTTGTGCTTCTGGAGTTACTCTCTGACAAGAAGGCACTGGATCCCTCTTTCTCTTCATATGGAAATGGATTCAACATCGTTCAATGGTCATGTATGCTTCTTCGGCAAGGCCGTGCAAAGGAATTCTTCACTGCTGGGTTATGGGATGCAGGCCCCCATGATGATTTAGTAGAAGTTCTGCACTTGGCAGTAGTGTGCACGGTTGACTCTCTCTCAACCAGGCCTACAATGAGGCAAGTTGTACGACGGCTTAAGCAACTTCAGCCTCCATCCTGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000578 GO:0001101 GO:0003002 GO:0003006 GO:0005575 GO:0005623 GO:0005886 GO:0006725 GO:0006950 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009555 GO:0009628 GO:0009698 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009808 GO:0009846 GO:0009856 GO:0009880 GO:0009888 GO:0009908 GO:0009942 GO:0009945 GO:0009956 GO:0009987 GO:0010035 GO:0010073 GO:0010152 GO:0010154 GO:0016020 GO:0016043 GO:0019748 GO:0021700 GO:0022414 GO:0022607 GO:0032501 GO:0032502 GO:0042221 GO:0043933 GO:0044085 GO:0044237 GO:0044464 GO:0044706 GO:0048229 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048507 GO:0048508 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051259 GO:0051260 GO:0051704 GO:0061458 GO:0065003 GO:0071704 GO:0071840 GO:0071944 GO:0090567 GO:0099402 GO:1901360 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1144

Amino Acids

123.87

Weight (kDa)

6.47

Isoelectric Point (pI)

37.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	46 - 86	1.1e-10	Leucine rich repeat N-terminal domain
LRR_14	PF23598	213 - 395	7.9e-09	Leucine-rich repeat region
LRR_14	PF23598	645 - 727	4.7e-08	Leucine-rich repeat region
LRR_8	PF13855	650 - 710	2.4e-09	Leucine rich repeat
Pkinase	PF00069	866 - 1065	1e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	867 - 1135	2.5e-39	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0010808)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02130 AT3G02130
fragaria_vesca	FvH4_6g46830
malus_domestica	MD09G1067100.v1.1 MD17G1060000.v1.1 MD17G1060100.v1.1
prunus_persica	Prupe.3G254600_v2.0.a1
pyrus_communis	pycom111g05640 pycom17g05970 pycom17g05980
rosa_chinensis	RchiOBHm_Chr2g0165821
rosa_laevigata	RLG00000021558
rosa_multiflora	Rmu_sc0003227.1_g000004
rosa_roxburghii	Rroxscaffold_2G00085480
rosa_rugosa	Rorug02G0518000
rosa_samantha	Rh2AG583700 Rh2BG595700 Rh2CG566600 Rh2DG605500
rosa_wichuraiana	Rw2G048670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	640, 2170
AatII	GACGTC	1 cut(s)	2967
AccB1I	GGYRCC	1 cut(s)	1180
AccB7I	CCANNNNNTGG	1 cut(s)	2495
AccI	GTMKAC	1 cut(s)	2471
AccII	CGCG	2 cut(s)	1589, 2961
AciI	CCGC	3 cut(s)	300, 356, 2822
AclWI	GGATC	8 cut(s)	176, 189, 1178, 1368, 1478, 1491, 3188, 3201
AcoI	YGGCCR	4 cut(s)	408, 1939, 2427, 3027
AcsI	RAATTY	7 cut(s)	169, 262, 489, 583, 1277, 1697, 3275
AcuI	CTGAAG	6 cut(s)	98, 171, 1295, 1809, 2289, 3398
AcvI	CACGTG	2 cut(s)	989, 2948
AcyI	GRCGYC	2 cut(s)	246, 2964
AdeI	CACNNNGTG	2 cut(s)	755, 1803
AfaI	GTAC	7 cut(s)	734, 1132, 1235, 1578, 2883, 2943, 3396
AfiI	CCNNNNNNNGG	6 cut(s)	362, 473, 2360, 2495, 2739, 3271
AflII	CTTAAG	1 cut(s)	3404
AflIII	ACRYGT	3 cut(s)	1404, 1908, 2947
AhlI	ACTAGT	1 cut(s)	1283
AjiI	CACGTC	1 cut(s)	1324
AjnI	CCWGG	4 cut(s)	703, 1702, 2653, 3371
Alw21I	GWGCWC	4 cut(s)	338, 1331, 2917, 3353
Alw26I	GTCTC	2 cut(s)	1884, 3044
Alw44I	GTGCAC	2 cut(s)	2913, 3349
AlwI	GGATC	8 cut(s)	176, 189, 1178, 1368, 1478, 1491, 3188, 3201
AlwNI	CAGNNNCTG	1 cut(s)	1621
Ama87I	CYCGRG	1 cut(s)	267
AoxI	GGCC	7 cut(s)	408, 1939, 2427, 3027, 3262, 3304, 3374
ApaLI	GTGCAC	2 cut(s)	2913, 3349
ApeKI	GCWGC	7 cut(s)	562, 861, 1299, 1621, 2014, 2246, 2345
ApoI	RAATTY	7 cut(s)	169, 262, 489, 583, 1277, 1697, 3275
ArsI	GACNNNNNNTTYG	2 cut(s)	30, 62
Asp700I	GAANNNNTTC	1 cut(s)	2387
AspLEI	GCGC	2 cut(s)	1591, 1610
AspS9I	GGNCC	4 cut(s)	240, 1850, 2363, 3305
AsuC2I	CCSGG	1 cut(s)	412
AsuHPI	GGTGA	8 cut(s)	497, 635, 1571, 1931, 2075, 2339, 2343, 2643
AvaI	CYCGRG	1 cut(s)	267
AvaII	GGWCC	3 cut(s)	240, 1850, 2363
BaeGI	GKGCMC	4 cut(s)	1183, 1205, 2917, 3353
BaeI	ACNNNNGTAYC	2 cut(s)	1056, 1089
BalI	TGGCCA	2 cut(s)	1941, 3029
BamHI	GGATCC	3 cut(s)	181, 1483, 3193
BanI	GGYRCC	1 cut(s)	1180
BanII	GRGCYC	1 cut(s)	1331
BauI	CACGAG	2 cut(s)	637, 1053
BbrPI	CACGTG	2 cut(s)	989, 2948
BbsI	GAAGAC	3 cut(s)	9, 241, 2381
Bbv12I	GWGCWC	4 cut(s)	338, 1331, 2917, 3353
BbvI	GCAGC	7 cut(s)	549, 873, 1286, 1608, 2026, 2233, 2332
BccI	CCATC	6 cut(s)	1495, 1874, 2061, 2152, 2757, 3430
BceAI	ACGGC	4 cut(s)	944, 3102, 3249, 3416
BciT130I	CCWGG	4 cut(s)	705, 1704, 2655, 3373
BclI	TGATCA	2 cut(s)	1245, 2932
BcnI	CCSGG	1 cut(s)	412
BcoDI	GTCTC	2 cut(s)	1884, 3044
BcuI	ACTAGT	1 cut(s)	1283
BfaI	CTAG	8 cut(s)	495, 659, 1284, 1643, 2004, 2267, 2514, 2598
BfmI	CTRYAG	4 cut(s)	106, 859, 1140, 1300
BfoI	RGCGCY	1 cut(s)	1611
BfrI	CTTAAG	1 cut(s)	3404
BglII	AGATCT	2 cut(s)	663, 2077
BisI	GCNGC	8 cut(s)	356, 563, 862, 1300, 1622, 2015, 2247, 2346
BlsI	GCNGC	8 cut(s)	357, 564, 863, 1301, 1623, 2016, 2248, 2347
BmcAI	AGTACT	1 cut(s)	1235
Bme1390I	CCNGG	5 cut(s)	412, 705, 1704, 2655, 3373
Bme18I	GGWCC	3 cut(s)	240, 1850, 2363
BmeT110I	CYCGRG	1 cut(s)	267
BmgBI	CACGTC	1 cut(s)	1324
BmgT120I	GGNCC	4 cut(s)	240, 1850, 2363, 3305
BmiI	GGNNCC	9 cut(s)	183, 697, 1182, 1485, 2550, 2578, 2696, 3195, 3307
BmrFI	CCNGG	5 cut(s)	412, 705, 1704, 2655, 3373
BmrI	ACTGGG	5 cut(s)	300, 564, 762, 1281, 2388
BmsI	GCATC	7 cut(s)	155, 811, 1816, 1838, 2417, 2432, 3289
BmuI	ACTGGG	5 cut(s)	300, 564, 762, 1281, 2388
BoxI	GACNNNNGTC	1 cut(s)	245
BpiI	GAAGAC	3 cut(s)	9, 241, 2381
BplI	GAGNNNNNCTC	4 cut(s)	530, 562, 3158, 3190
BpmI	CTGGAG	5 cut(s)	48, 1106, 1695, 1713, 3185
Bpu10I	CCTNAGC	2 cut(s)	449, 1166
BpuEI	CTTGAG	6 cut(s)	179, 557, 688, 818, 2298, 2487
BpuMI	CCSGG	1 cut(s)	412
BsaAI	YACGTR	2 cut(s)	989, 2948
BsaHI	GRCGYC	2 cut(s)	246, 2964
BsaI	GGTCTC	1 cut(s)	3044
BsaJI	CCNNGG	3 cut(s)	1184, 2698, 2734
Bsc4I	CCNNNNNNNGG	6 cut(s)	362, 473, 2360, 2495, 2739, 3271
Bse118I	RCCGGY	1 cut(s)	242
Bse3DI	GCAATG	4 cut(s)	229, 1538, 2248, 3114
BseBI	CCWGG	4 cut(s)	705, 1704, 2655, 3373
BseDI	CCNNGG	3 cut(s)	1184, 2698, 2734
BseGI	GGATG	7 cut(s)	958, 1487, 2408, 2749, 2995, 3304, 3422
BseLI	CCNNNNNNNGG	6 cut(s)	362, 473, 2360, 2495, 2739, 3271
BseMI	GCAATG	4 cut(s)	229, 1538, 2248, 3114
BseSI	GKGCMC	4 cut(s)	1183, 1205, 2917, 3353
BseXI	GCAGC	7 cut(s)	549, 873, 1286, 1608, 2026, 2233, 2332
BseYI	CCCAGC	3 cut(s)	677, 994, 3287
BsgI	GTGCAG	1 cut(s)	3317
Bsh1236I	CGCG	2 cut(s)	1589, 2961
Bsh1285I	CGRYCG	1 cut(s)	214
BshFI	GGCC	7 cut(s)	410, 1941, 2429, 3029, 3264, 3306, 3376
BshNI	GGYRCC	1 cut(s)	1180
BsiEI	CGRYCG	1 cut(s)	214
BsiHKAI	GWGCWC	4 cut(s)	338, 1331, 2917, 3353
BsiHKCI	CYCGRG	1 cut(s)	267
BsiSI	CCGG	2 cut(s)	243, 411
BslFI	GGGAC	1 cut(s)	445
BslI	CCNNNNNNNGG	6 cut(s)	362, 473, 2360, 2495, 2739, 3271
BsmAI	GTCTC	2 cut(s)	1884, 3044
BsmFI	GGGAC	1 cut(s)	445
BsmI	GAATGC	2 cut(s)	1102, 1664
BsnI	GGCC	7 cut(s)	410, 1941, 2429, 3029, 3264, 3306, 3376
Bso31I	GGTCTC	1 cut(s)	3044
BsoBI	CYCGRG	1 cut(s)	267
Bsp1286I	GDGCHC	6 cut(s)	338, 1183, 1205, 1331, 2917, 3353
Bsp68I	TCGCGA	1 cut(s)	2961
BspACI	CCGC	3 cut(s)	300, 356, 2822
BspANI	GGCC	7 cut(s)	410, 1941, 2429, 3029, 3264, 3306, 3376
BspFNI	CGCG	2 cut(s)	1589, 2961
BspLI	GGNNCC	9 cut(s)	183, 697, 1182, 1485, 2550, 2578, 2696, 3195, 3307
BspMAI	CTGCAG	2 cut(s)	863, 1304
BspPI	GGATC	8 cut(s)	176, 189, 1178, 1368, 1478, 1491, 3188, 3201
BspT107I	GGYRCC	1 cut(s)	1180
BspTI	CTTAAG	1 cut(s)	3404
BspTNI	GGTCTC	1 cut(s)	3044
BsrDI	GCAATG	4 cut(s)	229, 1538, 2248, 3114
BsrFI	RCCGGY	1 cut(s)	242
BssAI	RCCGGY	1 cut(s)	242
BssECI	CCNNGG	3 cut(s)	1184, 2698, 2734
BssNAI	GTATAC	1 cut(s)	2472
BssNI	GRCGYC	2 cut(s)	246, 2964
BssSI	CACGAG	2 cut(s)	637, 1053
BssT1I	CCWWGG	3 cut(s)	1184, 2698, 2734
Bst1107I	GTATAC	1 cut(s)	2472
Bst2BI	CACGAG	2 cut(s)	637, 1053
Bst2UI	CCWGG	4 cut(s)	705, 1704, 2655, 3373
Bst6I	CTCTTC	3 cut(s)	28, 897, 3211
BstACI	GRCGYC	2 cut(s)	246, 2964
BstAFI	CTTAAG	1 cut(s)	3404
BstAPI	GCANNNNNTGC	3 cut(s)	1621, 1670, 1803
BstBAI	YACGTR	2 cut(s)	989, 2948
BstC8I	GCNNGC	5 cut(s)	194, 334, 2217, 2301, 3304
BstF5I	GGATG	7 cut(s)	958, 1487, 2408, 2749, 2995, 3304, 3422
BstFNI	CGCG	2 cut(s)	1589, 2961
BstH2I	RGCGCY	1 cut(s)	1611
BstHHI	GCGC	2 cut(s)	1591, 1610
BstMAI	GTCTC	2 cut(s)	1884, 3044
BstMCI	CGRYCG	1 cut(s)	214
BstNI	CCWGG	4 cut(s)	705, 1704, 2655, 3373
BstNSI	RCATGY	2 cut(s)	1408, 1912
BstPAI	GACNNNNGTC	1 cut(s)	245
BstSCI	CCNGG	5 cut(s)	410, 703, 1702, 2653, 3371
BstSFI	CTRYAG	4 cut(s)	106, 859, 1140, 1300
BstSLI	GKGCMC	4 cut(s)	1183, 1205, 2917, 3353
BstUI	CGCG	2 cut(s)	1589, 2961
BstV1I	GCAGC	7 cut(s)	549, 873, 1286, 1608, 2026, 2233, 2332
BstV2I	GAAGAC	3 cut(s)	9, 241, 2381
BstX2I	RGATCY	7 cut(s)	181, 663, 1170, 1360, 1483, 2077, 3193
BstXI	CCANNNNNNTGG	4 cut(s)	1218, 2060, 2437, 3037
BstYI	RGATCY	7 cut(s)	181, 663, 1170, 1360, 1483, 2077, 3193
BstZ17I	GTATAC	1 cut(s)	2472
BsuRI	GGCC	7 cut(s)	410, 1941, 2429, 3029, 3264, 3306, 3376
BtgZI	GCGATG	1 cut(s)	2942
BtrI	CACGTC	1 cut(s)	1324
BtsCI	GGATG	7 cut(s)	958, 1487, 2408, 2749, 2995, 3304, 3422
BtsI	GCAGTG	3 cut(s)	735, 2210, 3282
BtuMI	TCGCGA	1 cut(s)	2961
Cac8I	GCNNGC	5 cut(s)	194, 334, 2217, 2301, 3304
CaiI	CAGNNNCTG	1 cut(s)	1621
CfoI	GCGC	2 cut(s)	1591, 1610
Cfr10I	RCCGGY	1 cut(s)	242
Cfr13I	GGNCC	4 cut(s)	240, 1850, 2363, 3305
CseI	GACGC	3 cut(s)	152, 235, 1627
Csp6I	GTAC	7 cut(s)	733, 1131, 1234, 1577, 2882, 2942, 3395
CspCI	CAANNNNNGTGG	4 cut(s)	1204, 1239, 2850, 2885
CviQI	GTAC	7 cut(s)	733, 1131, 1234, 1577, 2882, 2942, 3395
DraIII	CACNNNGTG	2 cut(s)	755, 1803
DrdI	GACNNNNNNGTC	2 cut(s)	640, 2170
DseDI	GACNNNNNNGTC	2 cut(s)	640, 2170
EaeI	YGGCCR	4 cut(s)	408, 1939, 2427, 3027
Eam1104I	CTCTTC	3 cut(s)	28, 897, 3211
EarI	CTCTTC	3 cut(s)	28, 897, 3211
EciI	GGCGGA	1 cut(s)	315
Ecl136II	GAGCTC	1 cut(s)	1329
Eco130I	CCWWGG	3 cut(s)	1184, 2698, 2734
Eco147I	AGGCCT	1 cut(s)	3376
Eco24I	GRGCYC	1 cut(s)	1331
Eco31I	GGTCTC	1 cut(s)	3044
Eco32I	GATATC	2 cut(s)	1393, 2649
Eco47I	GGWCC	3 cut(s)	240, 1850, 2363
Eco53kI	GAGCTC	1 cut(s)	1329
Eco57I	CTGAAG	6 cut(s)	98, 171, 1295, 1809, 2289, 3398
Eco72I	CACGTG	2 cut(s)	989, 2948
Eco88I	CYCGRG	1 cut(s)	267
EcoICRI	GAGCTC	1 cut(s)	1329
EcoO109I	RGGNCCY	1 cut(s)	3305
EcoRI	GAATTC	1 cut(s)	3275
EcoRII	CCWGG	4 cut(s)	703, 1702, 2653, 3371
EcoRV	GATATC	2 cut(s)	1393, 2649
EcoT14I	CCWWGG	3 cut(s)	1184, 2698, 2734
EcoT22I	ATGCAT	2 cut(s)	1666, 1831
EcoT38I	GRGCYC	1 cut(s)	1331
ErhI	CCWWGG	3 cut(s)	1184, 2698, 2734
FalI	AAGNNNNNCTT	2 cut(s)	3263, 3295
FaqI	GGGAC	1 cut(s)	445
FauI	CCCGC	1 cut(s)	2815
FauNDI	CATATG	1 cut(s)	3211
FbaI	TGATCA	2 cut(s)	1245, 2932
FblI	GTMKAC	1 cut(s)	2471
Fnu4HI	GCNGC	8 cut(s)	356, 563, 862, 1300, 1622, 2015, 2247, 2346
FokI	GGATG	7 cut(s)	965, 1474, 2395, 2736, 3002, 3311, 3409
FriOI	GRGCYC	1 cut(s)	1331
Fsp4HI	GCNGC	8 cut(s)	356, 563, 862, 1300, 1622, 2015, 2247, 2346
FspBI	CTAG	8 cut(s)	495, 659, 1284, 1643, 2004, 2267, 2514, 2598
GlaI	GCGC	2 cut(s)	1590, 1609
GluI	GCNGC	8 cut(s)	356, 563, 862, 1300, 1622, 2015, 2247, 2346
GsaI	CCCAGC	3 cut(s)	681, 998, 3291
GsuI	CTGGAG	5 cut(s)	48, 1106, 1695, 1713, 3185
HaeII	RGCGCY	1 cut(s)	1611
HaeIII	GGCC	7 cut(s)	410, 1941, 2429, 3029, 3264, 3306, 3376
HapII	CCGG	2 cut(s)	243, 411
HgaI	GACGC	3 cut(s)	152, 235, 1627
HhaI	GCGC	2 cut(s)	1591, 1610
Hin1I	GRCGYC	2 cut(s)	246, 2964
Hin6I	GCGC	2 cut(s)	1589, 1608
HinP1I	GCGC	2 cut(s)	1589, 1608
HincII	GTYRAC	2 cut(s)	2859, 3358
HindII	GTYRAC	2 cut(s)	2859, 3358
HindIII	AAGCTT	3 cut(s)	80, 794, 1307
HpaII	CCGG	2 cut(s)	243, 411
HphI	GGTGA	8 cut(s)	497, 635, 1571, 1931, 2075, 2339, 2343, 2643
Hpy99I	CGWCG	2 cut(s)	2966, 3402
HpyCH4IV	ACGT	5 cut(s)	875, 988, 1323, 2947, 2964
HpySE526I	ACGT	5 cut(s)	875, 988, 1323, 2947, 2964
Hsp92I	GRCGYC	2 cut(s)	246, 2964
HspAI	GCGC	2 cut(s)	1589, 1608
Ksp22I	TGATCA	2 cut(s)	1245, 2932
LmnI	GCTCC	8 cut(s)	116, 381, 423, 779, 1087, 1348, 2576, 2627
Lsp1109I	GCAGC	7 cut(s)	549, 873, 1286, 1608, 2026, 2233, 2332
LweI	GCATC	7 cut(s)	155, 811, 1816, 1838, 2417, 2432, 3289
MaeI	CTAG	8 cut(s)	495, 659, 1284, 1643, 2004, 2267, 2514, 2598
MaeII	ACGT	5 cut(s)	875, 988, 1323, 2947, 2964
MaeIII	GTNAC	8 cut(s)	550, 1058, 1559, 2161, 2524, 2600, 2761, 3168
MfeI	CAATTG	2 cut(s)	1216, 2397
MflI	RGATCY	7 cut(s)	181, 663, 1170, 1360, 1483, 2077, 3193
MhlI	GDGCHC	6 cut(s)	338, 1183, 1205, 1331, 2917, 3353
MlsI	TGGCCA	2 cut(s)	1941, 3029
MluNI	TGGCCA	2 cut(s)	1941, 3029
MlyI	GAGTC	3 cut(s)	1070, 2264, 3353
MmeI	TCCRAC	5 cut(s)	930, 1510, 1883, 2668, 2967
Mox20I	TGGCCA	2 cut(s)	1941, 3029
Mph1103I	ATGCAT	2 cut(s)	1666, 1831
MroXI	GAANNNNTTC	1 cut(s)	2387
MscI	TGGCCA	2 cut(s)	1941, 3029
MslI	CAYNNNNRTG	2 cut(s)	2479, 2784
Msp20I	TGGCCA	2 cut(s)	1941, 3029
MspA1I	CMGCKG	3 cut(s)	917, 1787, 2017
MspCI	CTTAAG	1 cut(s)	3404
MspI	CCGG	2 cut(s)	243, 411
MspR9I	CCNGG	5 cut(s)	412, 705, 1704, 2655, 3373
MunI	CAATTG	2 cut(s)	1216, 2397
Mva1269I	GAATGC	2 cut(s)	1102, 1664
MvaI	CCWGG	4 cut(s)	705, 1704, 2655, 3373
MvnI	CGCG	2 cut(s)	1589, 2961
NciI	CCSGG	1 cut(s)	412
NdeI	CATATG	1 cut(s)	3211
NlaIV	GGNNCC	9 cut(s)	183, 697, 1182, 1485, 2550, 2578, 2696, 3195, 3307
NmeAIII	GCCGAG	1 cut(s)	2997
NmuCI	GTSAC	3 cut(s)	1559, 2161, 2761
NruI	TCGCGA	1 cut(s)	2961
NsiI	ATGCAT	2 cut(s)	1666, 1831
NspI	RCATGY	2 cut(s)	1408, 1912
PaeR7I	CTCGAG	1 cut(s)	267
PceI	AGGCCT	1 cut(s)	3376
PciI	ACATGT	2 cut(s)	1404, 1908
PcsI	WCGNNNNNNNCGW	1 cut(s)	3262
PctI	GAATGC	2 cut(s)	1102, 1664
PdmI	GAANNNNTTC	1 cut(s)	2387
PflFI	GACNNNGTC	1 cut(s)	1293
PflMI	CCANNNNNTGG	1 cut(s)	2495
PkrI	GCNGC	8 cut(s)	357, 564, 863, 1301, 1623, 2016, 2248, 2347
PleI	GAGTC	3 cut(s)	1070, 2264, 3353
PmaCI	CACGTG	2 cut(s)	989, 2948
PmlI	CACGTG	2 cut(s)	989, 2948
PpsI	GAGTC	3 cut(s)	1070, 2264, 3353
Ppu21I	YACGTR	2 cut(s)	989, 2948
PscI	ACATGT	2 cut(s)	1404, 1908
PshAI	GACNNNNGTC	1 cut(s)	245
Psp124BI	GAGCTC	1 cut(s)	1331
Psp6I	CCWGG	4 cut(s)	703, 1702, 2653, 3371
PspCI	CACGTG	2 cut(s)	989, 2948
PspFI	CCCAGC	3 cut(s)	677, 994, 3287
PspGI	CCWGG	4 cut(s)	703, 1702, 2653, 3371
PspN4I	GGNNCC	9 cut(s)	183, 697, 1182, 1485, 2550, 2578, 2696, 3195, 3307
PspPI	GGNCC	4 cut(s)	240, 1850, 2363, 3305
PstI	CTGCAG	2 cut(s)	863, 1304
PstNI	CAGNNNCTG	1 cut(s)	1621
PsuI	RGATCY	7 cut(s)	181, 663, 1170, 1360, 1483, 2077, 3193
PsyI	GACNNNGTC	1 cut(s)	1293
PvuII	CAGCTG	3 cut(s)	917, 1787, 2017
RruI	TCGCGA	1 cut(s)	2961
RsaI	GTAC	7 cut(s)	734, 1132, 1235, 1578, 2883, 2943, 3396
RsaNI	GTAC	7 cut(s)	733, 1131, 1234, 1577, 2882, 2942, 3395
RseI	CAYNNNNRTG	2 cut(s)	2479, 2784
SacI	GAGCTC	1 cut(s)	1331
SatI	GCNGC	8 cut(s)	356, 563, 862, 1300, 1622, 2015, 2247, 2346
Sau96I	GGNCC	4 cut(s)	240, 1850, 2363, 3305
ScaI	AGTACT	1 cut(s)	1235
SchI	GAGTC	3 cut(s)	1070, 2264, 3353
ScrFI	CCNGG	5 cut(s)	412, 705, 1704, 2655, 3373
SduI	GDGCHC	6 cut(s)	338, 1183, 1205, 1331, 2917, 3353
SfaNI	GCATC	7 cut(s)	155, 811, 1816, 1838, 2417, 2432, 3289
SfcI	CTRYAG	4 cut(s)	106, 859, 1140, 1300
Sfr274I	CTCGAG	1 cut(s)	267
SinI	GGWCC	3 cut(s)	240, 1850, 2363
SlaI	CTCGAG	1 cut(s)	267
SmiMI	CAYNNNNRTG	2 cut(s)	2479, 2784
SmlI	CTYRAG	8 cut(s)	158, 267, 536, 667, 797, 2313, 2502, 3404
SmoI	CTYRAG	8 cut(s)	158, 267, 536, 667, 797, 2313, 2502, 3404
SpeI	ACTAGT	1 cut(s)	1283
SseBI	AGGCCT	1 cut(s)	3376
SsiI	CCGC	3 cut(s)	300, 356, 2822
SspI	AATATT	3 cut(s)	1430, 2128, 2980
SspMI	CTAG	8 cut(s)	495, 659, 1284, 1643, 2004, 2267, 2514, 2598
SstI	GAGCTC	1 cut(s)	1331
StuI	AGGCCT	1 cut(s)	3376
StyD4I	CCNGG	5 cut(s)	410, 703, 1702, 2653, 3371
StyI	CCWWGG	3 cut(s)	1184, 2698, 2734
TaiI	ACGT	5 cut(s)	878, 991, 1326, 2950, 2967
TaqI	TCGA	4 cut(s)	268, 320, 1094, 2115
TaqII	GACCGA	1 cut(s)	16
TatI	WGTACW	3 cut(s)	732, 1233, 1576
TauI	GCSGC	1 cut(s)	358
TseFI	GTSAC	3 cut(s)	1559, 2161, 2761
TseI	GCWGC	7 cut(s)	562, 861, 1299, 1621, 2014, 2246, 2345
Tsp45I	GTSAC	3 cut(s)	1559, 2161, 2761
TspDTI	ATGAA	9 cut(s)	700, 821, 969, 1118, 1355, 2249, 2705, 2945, 3198
TspGWI	ACGGA	2 cut(s)	1989, 2491
Tth111I	GACNNNGTC	1 cut(s)	1293
Van91I	CCANNNNNTGG	1 cut(s)	2495
Vha464I	CTTAAG	1 cut(s)	3404
VneI	GTGCAC	2 cut(s)	2913, 3349
VpaK11BI	GGWCC	3 cut(s)	240, 1850, 2363
XapI	RAATTY	7 cut(s)	169, 262, 489, 583, 1277, 1697, 3275
XbaI	TCTAGA	1 cut(s)	2266
XceI	RCATGY	2 cut(s)	1408, 1912
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2287, 3062
XhoI	CTCGAG	1 cut(s)	267
XmiI	GTMKAC	1 cut(s)	2471
XmnI	GAANNNNTTC	1 cut(s)	2387
XspI	CTAG	8 cut(s)	495, 659, 1284, 1643, 2004, 2267, 2514, 2598
ZraI	GACGTC	1 cut(s)	2965
ZrmI	AGTACT	1 cut(s)	1235
Zsp2I	ATGCAT	2 cut(s)	1666, 1831

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.