Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0010808

Physical Location & Seq

Genomic Coordinates Reverse (-)

76387267 .. 76391705

Gene Structure 4439 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000021558

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3504 bp

ATGGGTGTTCGATCTTCTTCCTCTCCGGTCACCAATTTCCTGTCTTTTCCGGGACCCACTTCGCCGCTGGTTCTGAAGCTCGTTTTGGTGCTGTGGGTGTGGGCGGCGGCGTCACAGAAGGGCGTCGTTTCGGCGGAGACGGACGCATCGGTTCTGCTTCAGCTCAAAGGCTCAGTCTTGGACTCGGAAGGGTTGCTTTCGAGCTGGGGCTCGGTCAATTCCAGTCACTGCACGTGGTCCGGAGTCTTGTGCGACGCGAATTTCCGTGTCGTTTCGCTCAACATTACCGGCGGCGGCGTCGGAGGCGGAGGTAAATCGAAATCGAGCTCCTTTCCGTGCGTTGATTTTGGTCAGTTTCCGTTGTATGGGTTGGGGATTAGGAGGAGTTGTGTGGGTGGTAGTGGGAGGTTGGTGGGGAAGCTCCCCTCTGTGATTGGGAAGCTCACTGAGCTAAAGGTCTTGTCTTTGCCCTTTAATGGTTTTGATGGTGAAATTCCTGATGAAATTTGGGGCATGGAGAACCTAGAGGTCGTTGATCTTGAAGGAAATTCGATGACCGGGTCTTTGCCGTTTCGGGTGAACCCGAATTTGAGGGTTCTGAATCTTGGGTTTAATAAGATACACGGTGAGATACCGATCTTTGACTCTGTCAGCTTAGAGATCTTGAATTTAGCTGGGAACCGGGTGAACGGCTCCGTTCCGGGTTTCGTTGGCAGGTTGAAAGGGGTGTACTTGTCATACAATTGGCTCAGTGGTGATATTCCTAGTGAGATTGGAGATAGTTGTGGGAAGCTTGAGCATCTTGATTTGTCTGGTAACTTCTTGGTTCATAAGATTCCGAGTAGTTTGGGGAACTGCAGTCGGTTGAGGACGCTGTTGTTGTATTCGAATATGTTGGAAGAAGGTATTCCGGCTGAACTCGGCCGGCTTCAGGTGCTTGAGGTGTTGGATGTGTCGAGGAATAGCCTGAGTGGTTCGTTGCCTCGAGAGCTGGGGAATTGTTCTCAGTTGTCTGTCCTTGTGCTTTCGTCGTTGTTTAATCCCCTTCCCCCTGTGCGAGCCAATTATACAGATGACTCGTTGTTGGAGCAATTGAGTTCCATGAATGATGACTTCAATTACTTTCAAGGCTCAATGCCGAAGGAGATTACAAGCCTTCCAAAGCTGAAGATCTTGTGGGGACCGAGAGCAAGTATTGAGGGCAGCTTTCCAAGTGATTGGGGTGCTTGTGAGAACTTGGAAATGATCAATTTGGCTCAGAATTTTTTTACTGGGGGAATTTCTAGTGGCCTTAACGGCTGCCTGAAGCTTCATTTTCTTGACCTAAGCACTAACAAGCTTACTGGGGAGCTTGGTCAGTTTCTTCAGGTTCCTTGTATGACTATGCTTGATGTCAGCGGAAATTTCTTGTCAGGTTCAATGCCTGAATATGCTAACAGTACTTGTCGTCCTGTTTCCTCTGTGGATCTTTCTTTCAAAGATGATGATTTTTCCTCGCCCTATGAAGAATTTTTTGGATCTAAAGCTCAAGCTGGAATGCCATTGCAATGGCCCACAGAGGATGATGTTGTTGTAGTGATTCACAACTTTGGGCACAATAATTTTACCGGCACTCTGCAGTCATTGCCCATTGCACCCGAAAGGTTTCAGAAGAAAATTCTGTATGCATTCCTTGTTGGAGAAAACAAGCTTACTGGAGCATTTCCAGGTAATTTGTTTGGGAAGTGTCAGGCATTAGATTCACTGGTTGTTAATGTAAGCAACAACAGGTTAGATGGGAAGATTCCAACTGAAATTGGTAAAATGTGCAATTCTCTCAAGTTTTTAGATGCATCTCTGAATCGGATTGTGGGTTCCATTCCTGCTAGTTTTGGAGAATTGGTGTCTCTTGCTGGCCTTAACTTGAGTTCGAACAGGTTGCAAGGTCAAATTCCGACCACTCTTGGCCAGATAAGGGATCTGGAGTATCTTTCTTTGTCTGGTAATAACCTTACAGGTGTTATTCCTGCTAGTTTGGGGCAGCTTTACTCCTTGCATGTGCTTGAGCTTTCTCGGAACTCTCTCACTGGTGAGATACCAAAAGATCTTGTAAACTTGCAAAACTTGAGAGTTCTTCTGCTGGACAAAAATAAACTTTCTGGCCAGATTCCATCTGGTTTGGCAAATGTGACCACGCTCTCAGCCTTCAATGTGTCTTATAATAACTTCTCTGGTTCATTGCCTTTGAACAATAACCTGGTGAATTGCAATACTGCTATTGGAAACCCGCATTTAAGTTCCTGTCCCTCACTTTCTCAAGTACAGCCAGCTAATACTCAAGGAAGAGTTGGCGATTCAGAACCTTATGCTGCTTCACCAGTGGTAGGAACTGAGAAGAGTGCTGGTAGCAGTTTCAATTCAATTGAAATAGCATCCATTACATCTGCATCTGCCATTGTTTTAGTGCTTCTAGCTCTTGTTGTTCTATTCCTGTATACACGGAAGTGGAATCGGAAGTCTGGAGGTCCAGGCTCTACTAGGAAGGAAGTTACCGTTTTCACAAACATTGGGGTTCCACTGACCTTTGATAGTGTTGTTCAGGGCACGGGGAGCTTCAATGCTAGCAACTGTATTGGGAATGGAGGTTTTGGAGCAACTTACAAGGCAGAGATATCACCTGGAGTCCTAGTGGCTATAAAACGGCTTGCTGTTGGAAGGTTCCAAGGAGTGCAACAGTTTCATGCAGAAATCAAGACTCTGGGGAGGCTTCGACATCCAAATCTGGTCACTTTACTAGGTTACCATGCCAGTGAAACAGAGATGTTCCTAATATATAATTATTTTCCTGGTGGTAATCTGGAAAAATTTATTCAGGAAAGGTCTACCAGGGCTGTGGATTGGAAGATACTTCACAAGATTGCTTTGGACATAGCCCGTGCACTTGCCTACCTACATGACCAGTGTGTACCTCGTGTCCTTCACCGTGATGTCAAGCCCAGCAATATTTTGTTGGATGATGATTTTAATGCATATCTATCAGACTTTGGTTTGGCCAGACTTCTTGGGACTTCAGAAACCCATGCTACTACTGGTGTAGCTGGGACCTTTGGATATGTTGCCCCAGAATATGCAATGACGTGTCGTGTTTCAGATAAGTCTGATGTGTATAGTTATGGTGTAGTGCTTCTTGAGTTGCTTTCTGACAAGAAAGTGTTGGATCCTTCCTTTTCTTCATATGGAAATGGATTCAACATCGTTGCATGGGCATGTATGCTTCTTCGACAAGGCCGTGCAAAGGAATTCTTTTCTGCAGGGTTGTGGGATGCAGGCCCCCATGATGATTTGGTTGAAGTTCTACACTTGGCAGTGAGTCTAACATGTCTTAGAAGGATGGCAAGTCGTGATCCTCGCGGTGGTGCAAGCAATAGCAATGAAGCAATAGACCAAGTAAAGCAGCAGTCGAAGCAAGTGAAACACGAGAAGCAGGAGATGCTGCCTAAGGAGTTGGAGCCTCAAATTCGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000578 GO:0001101 GO:0003002 GO:0003006 GO:0005575 GO:0005623 GO:0005886 GO:0006725 GO:0006950 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009555 GO:0009628 GO:0009698 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009808 GO:0009846 GO:0009856 GO:0009880 GO:0009888 GO:0009908 GO:0009942 GO:0009945 GO:0009956 GO:0009987 GO:0010035 GO:0010073 GO:0010152 GO:0010154 GO:0016020 GO:0016043 GO:0019748 GO:0021700 GO:0022414 GO:0022607 GO:0032501 GO:0032502 GO:0042221 GO:0043933 GO:0044085 GO:0044237 GO:0044464 GO:0044706 GO:0048229 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048507 GO:0048508 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051259 GO:0051260 GO:0051704 GO:0061458 GO:0065003 GO:0071704 GO:0071840 GO:0071944 GO:0090567 GO:0099402 GO:1901360 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1168

Amino Acids

125.98

Weight (kDa)

6.15

Isoelectric Point (pI)

36.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	46 - 85	9.2e-10	Leucine rich repeat N-terminal domain
LRR_14	PF23598	268 - 392	3e-07	Leucine-rich repeat region
LRR_14	PF23598	644 - 729	7.9e-08	Leucine-rich repeat region
LRR_8	PF13855	654 - 712	1.6e-08	Leucine rich repeat
Pkinase	PF00069	867 - 1066	2.4e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	868 - 1061	6.6e-39	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010808)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02130 AT3G02130
fragaria_vesca	FvH4_6g46830
malus_domestica	MD09G1067100.v1.1 MD17G1060000.v1.1 MD17G1060100.v1.1
prunus_persica	Prupe.3G254600_v2.0.a1
pyrus_communis	pycom111g05640 pycom17g05970 pycom17g05980
rosa_chinensis	RchiOBHm_Chr2g0165821
rosa_laevigata	RLG00000021558
rosa_multiflora	Rmu_sc0003227.1_g000004
rosa_roxburghii	Rroxscaffold_2G00085480
rosa_rugosa	Rorug02G0518000
rosa_samantha	Rh2AG583700 Rh2BG595700 Rh2CG566600 Rh2DG605500
rosa_wichuraiana	Rw2G048670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2199
Acc36I	ACCTGC	1 cut(s)	705
AccI	GTMKAC	2 cut(s)	2474, 2861
AccII	CGCG	2 cut(s)	257, 3390
AccIII	TCCGGA	1 cut(s)	239
AclWI	GGATC	6 cut(s)	1473, 1525, 1965, 3191, 3204, 3377
AcoI	YGGCCR	4 cut(s)	922, 1945, 2140, 3030
AcuI	CTGAAG	7 cut(s)	95, 143, 914, 1187, 1325, 1349, 3033
AcvI	CACGTG	1 cut(s)	234
AcyI	GRCGYC	3 cut(s)	110, 123, 297
AfaI	GTAC	4 cut(s)	731, 1441, 2301, 2946
AfiI	CCNNNNNNNGG	7 cut(s)	365, 476, 931, 1954, 2363, 3274, 3392
AflIII	ACRYGT	2 cut(s)	3116, 3356
AjiI	CACGTC	1 cut(s)	3117
AjnI	CCWGG	6 cut(s)	1705, 2235, 2506, 2656, 2824, 2864
AjuI	GAANNNNNNNTTGG	4 cut(s)	68, 100, 1497, 1529
AleI	CACNNNNGTG	1 cut(s)	2482
AloI	GAACNNNNNNTCC	2 cut(s)	1837, 1869
Alw21I	GWGCWC	2 cut(s)	329, 2920
Alw26I	GTCTC	2 cut(s)	131, 1890
Alw44I	GTGCAC	1 cut(s)	2916
AlwI	GGATC	6 cut(s)	1473, 1525, 1965, 3191, 3204, 3377
AlwNI	CAGNNNCTG	1 cut(s)	228
Ama87I	CYCGRG	1 cut(s)	984
Aor13HI	TCCGGA	1 cut(s)	239
AoxI	GGCC	9 cut(s)	922, 1288, 1550, 1894, 1945, 2140, 3030, 3265, 3307
ApaLI	GTGCAC	1 cut(s)	2916
ApeKI	GCWGC	6 cut(s)	1203, 1299, 2020, 2348, 3433, 3472
ArsI	GACNNNNNNTTYG	4 cut(s)	159, 191, 547, 579
Asp700I	GAANNNNTTC	2 cut(s)	906, 1644
AspS9I	GGNCC	7 cut(s)	53, 237, 1181, 1551, 2504, 3081, 3308
AsuC2I	CCSGG	4 cut(s)	51, 559, 683, 702
AsuII	TTCGAA	2 cut(s)	887, 1910
AsuNHI	GCTAGC	1 cut(s)	2600
AvaI	CYCGRG	1 cut(s)	984
AvaII	GGWCC	5 cut(s)	53, 237, 1181, 2504, 3081
AxyI	CCTNAGG	1 cut(s)	3477
BaeGI	GKGCMC	3 cut(s)	1596, 2585, 2920
BalI	TGGCCA	3 cut(s)	1947, 2142, 3032
BamHI	GGATCC	1 cut(s)	3196
BanII	GRGCYC	2 cut(s)	212, 329
BauI	CACGAG	2 cut(s)	2949, 3455
BbrPI	CACGTG	1 cut(s)	234
Bbv12I	GWGCWC	2 cut(s)	329, 2920
BbvI	GCAGC	6 cut(s)	1215, 1286, 2032, 2335, 3445, 3459
BccI	CCATC	4 cut(s)	479, 1769, 2158, 3364
BceAI	ACGGC	5 cut(s)	553, 706, 1312, 2696, 3252
BcgI	CGANNNNNNTGC	2 cut(s)	1900, 1934
BciT130I	CCWGG	6 cut(s)	1707, 2237, 2508, 2658, 2826, 2866
BclI	TGATCA	1 cut(s)	1245
BcnI	CCSGG	4 cut(s)	51, 559, 683, 702
BcoDI	GTCTC	2 cut(s)	131, 1890
BfmI	CTRYAG	3 cut(s)	856, 1616, 3288
BfuAI	ACCTGC	1 cut(s)	705
BglI	GCCNNNNNGGC	1 cut(s)	1296
BglII	AGATCT	3 cut(s)	660, 1170, 2083
BmcAI	AGTACT	1 cut(s)	1441
Bme18I	GGWCC	5 cut(s)	53, 237, 1181, 2504, 3081
BmeT110I	CYCGRG	1 cut(s)	984
BmgBI	CACGTC	1 cut(s)	3117
BmgT120I	GGNCC	7 cut(s)	53, 237, 1181, 1551, 2504, 3081, 3308
BmrI	ACTGGG	2 cut(s)	1281, 1353
BmsI	GCATC	8 cut(s)	155, 808, 1819, 1841, 2420, 2435, 3292, 3459
BmtI	GCTAGC	1 cut(s)	2604
BmuI	ACTGGG	2 cut(s)	1281, 1353
BpmI	CTGGAG	4 cut(s)	1716, 1982, 2520, 2679
Bpu10I	CCTNAGC	1 cut(s)	1325
Bpu14I	TTCGAA	2 cut(s)	887, 1910
BpuMI	CCSGG	4 cut(s)	51, 559, 683, 702
BsaAI	YACGTR	1 cut(s)	234
BsaBI	GATNNNNATC	1 cut(s)	635
BsaHI	GRCGYC	3 cut(s)	110, 123, 297
BsaJI	CCNNGG	2 cut(s)	2701, 2865
BsaWI	WCCGGW	2 cut(s)	25, 239
BsaXI	ACNNNNNCTCC	6 cut(s)	373, 403, 1866, 1896, 2652, 2682
Bsc4I	CCNNNNNNNGG	7 cut(s)	365, 476, 931, 1954, 2363, 3274, 3392
Bse118I	RCCGGY	3 cut(s)	287, 924, 1607
Bse21I	CCTNAGG	1 cut(s)	3477
Bse3DI	GCAATG	7 cut(s)	1541, 1553, 1622, 1629, 2216, 3117, 3415
Bse8I	GATNNNNATC	1 cut(s)	635
BseAI	TCCGGA	1 cut(s)	239
BseBI	CCWGG	6 cut(s)	1707, 2237, 2508, 2658, 2826, 2866
BseDI	CCNNGG	2 cut(s)	2701, 2865
BseGI	GGATG	7 cut(s)	955, 1567, 2411, 2752, 2998, 3307, 3375
BseJI	GATNNNNATC	1 cut(s)	635
BseLI	CCNNNNNNNGG	7 cut(s)	365, 476, 931, 1954, 2363, 3274, 3392
BseMI	GCAATG	7 cut(s)	1541, 1553, 1622, 1629, 2216, 3117, 3415
BseMII	CTCAG	8 cut(s)	186, 438, 763, 959, 1019, 1271, 2193, 2361
BseRI	GAGGAG	1 cut(s)	397
BseSI	GKGCMC	3 cut(s)	1596, 2585, 2920
BseX3I	CGGCCG	1 cut(s)	922
BseXI	GCAGC	6 cut(s)	1215, 1286, 2032, 2335, 3445, 3459
BseYI	CCCAGC	5 cut(s)	204, 674, 991, 2975, 3077
BsgI	GTGCAG	1 cut(s)	214
Bsh1236I	CGCG	2 cut(s)	257, 3390
Bsh1285I	CGRYCG	1 cut(s)	925
BshFI	GGCC	9 cut(s)	924, 1290, 1552, 1896, 1947, 2142, 3032, 3267, 3309
BsiEI	CGRYCG	1 cut(s)	925
BsiHKAI	GWGCWC	2 cut(s)	329, 2920
BsiHKCI	CYCGRG	1 cut(s)	984
BslFI	GGGAC	5 cut(s)	66, 1194, 2268, 3058, 3094
BslI	CCNNNNNNNGG	7 cut(s)	365, 476, 931, 1954, 2363, 3274, 3392
BsmAI	GTCTC	2 cut(s)	131, 1890
BsmBI	CGTCTC	1 cut(s)	131
BsmFI	GGGAC	5 cut(s)	66, 1194, 2268, 3058, 3094
BsmI	GAATGC	2 cut(s)	1542, 1667
BsnI	GGCC	9 cut(s)	924, 1290, 1552, 1896, 1947, 2142, 3032, 3267, 3309
BsoBI	CYCGRG	1 cut(s)	984
Bsp119I	TTCGAA	2 cut(s)	887, 1910
Bsp1286I	GDGCHC	5 cut(s)	212, 329, 1596, 2585, 2920
Bsp13I	TCCGGA	1 cut(s)	239
BspANI	GGCC	9 cut(s)	924, 1290, 1552, 1896, 1947, 2142, 3032, 3267, 3309
BspCNI	CTCAG	8 cut(s)	185, 439, 762, 960, 1018, 1270, 2192, 2362
BspEI	TCCGGA	1 cut(s)	239
BspFNI	CGCG	2 cut(s)	257, 3390
BspMAI	CTGCAG	3 cut(s)	860, 1620, 3292
BspMI	ACCTGC	1 cut(s)	705
BspOI	GCTAGC	1 cut(s)	2604
BspPI	GGATC	6 cut(s)	1473, 1525, 1965, 3191, 3204, 3377
BspT104I	TTCGAA	2 cut(s)	887, 1910
BsrDI	GCAATG	7 cut(s)	1541, 1553, 1622, 1629, 2216, 3117, 3415
BsrFI	RCCGGY	3 cut(s)	287, 924, 1607
BssAI	RCCGGY	3 cut(s)	287, 924, 1607
BssECI	CCNNGG	2 cut(s)	2701, 2865
BssNAI	GTATAC	1 cut(s)	2475
BssNI	GRCGYC	3 cut(s)	110, 123, 297
BssSI	CACGAG	2 cut(s)	2949, 3455
BssT1I	CCWWGG	1 cut(s)	2701
Bst1107I	GTATAC	1 cut(s)	2475
Bst2BI	CACGAG	2 cut(s)	2949, 3455
Bst2UI	CCWGG	6 cut(s)	1707, 2237, 2508, 2658, 2826, 2866
Bst4CI	ACNGT	6 cut(s)	626, 1439, 2533, 2609, 2715, 2963
Bst6I	CTCTTC	2 cut(s)	2317, 2369
BstACI	GRCGYC	3 cut(s)	110, 123, 297
BstAPI	GCANNNNNTGC	2 cut(s)	1624, 3469
BstBAI	YACGTR	1 cut(s)	234
BstBI	TTCGAA	2 cut(s)	887, 1910
BstC8I	GCNNGC	8 cut(s)	926, 1059, 1894, 2307, 2602, 2685, 3307, 3400
BstEII	GGTNACC	2 cut(s)	28, 2777
BstF5I	GGATG	7 cut(s)	955, 1567, 2411, 2752, 2998, 3307, 3375
BstFNI	CGCG	2 cut(s)	257, 3390
BstMAI	GTCTC	2 cut(s)	131, 1890
BstMCI	CGRYCG	1 cut(s)	925
BstMWI	GCNNNNNNNGC	9 cut(s)	303, 448, 934, 988, 1296, 1624, 2589, 3442, 3469
BstNI	CCWGG	6 cut(s)	1707, 2237, 2508, 2658, 2826, 2866
BstNSI	RCATGY	3 cut(s)	2039, 3249, 3360
BstPI	GGTNACC	2 cut(s)	28, 2777
BstSFI	CTRYAG	3 cut(s)	856, 1616, 3288
BstSLI	GKGCMC	3 cut(s)	1596, 2585, 2920
BstUI	CGCG	2 cut(s)	257, 3390
BstV1I	GCAGC	6 cut(s)	1215, 1286, 2032, 2335, 3445, 3459
BstX2I	RGATCY	7 cut(s)	660, 1170, 1465, 1517, 1957, 2083, 3196
BstXI	CCANNNNNNTGG	3 cut(s)	1218, 1548, 2872
BstYI	RGATCY	7 cut(s)	660, 1170, 1465, 1517, 1957, 2083, 3196
BstZ17I	GTATAC	1 cut(s)	2475
BstZI	CGGCCG	1 cut(s)	922
Bsu36I	CCTNAGG	1 cut(s)	3477
BsuRI	GGCC	9 cut(s)	924, 1290, 1552, 1896, 1947, 2142, 3032, 3267, 3309
BtrI	CACGTC	1 cut(s)	3117
BtsCI	GGATG	7 cut(s)	955, 1567, 2411, 2752, 2998, 3307, 3375
BtsI	GCAGTG	2 cut(s)	226, 3351
BveI	ACCTGC	1 cut(s)	705
Cac8I	GCNNGC	8 cut(s)	926, 1059, 1894, 2307, 2602, 2685, 3307, 3400
CaiI	CAGNNNCTG	1 cut(s)	228
Cfr10I	RCCGGY	3 cut(s)	287, 924, 1607
Cfr13I	GGNCC	7 cut(s)	53, 237, 1181, 1551, 2504, 3081, 3308
CseI	GACGC	6 cut(s)	99, 112, 152, 263, 286, 880
CsiI	ACCWGGT	1 cut(s)	2235
Csp6I	GTAC	4 cut(s)	730, 1440, 2300, 2945
CviQI	GTAC	4 cut(s)	730, 1440, 2300, 2945
EaeI	YGGCCR	4 cut(s)	922, 1945, 2140, 3030
EagI	CGGCCG	1 cut(s)	922
Eam1104I	CTCTTC	2 cut(s)	2317, 2369
EarI	CTCTTC	2 cut(s)	2317, 2369
EciI	GGCGGA	2 cut(s)	149, 321
Ecl136II	GAGCTC	1 cut(s)	327
EclXI	CGGCCG	1 cut(s)	922
Eco130I	CCWWGG	1 cut(s)	2701
Eco24I	GRGCYC	2 cut(s)	212, 329
Eco32I	GATATC	1 cut(s)	2652
Eco47I	GGWCC	5 cut(s)	53, 237, 1181, 2504, 3081
Eco52I	CGGCCG	1 cut(s)	922
Eco53kI	GAGCTC	1 cut(s)	327
Eco57I	CTGAAG	7 cut(s)	95, 143, 914, 1187, 1325, 1349, 3033
Eco72I	CACGTG	1 cut(s)	234
Eco81I	CCTNAGG	1 cut(s)	3477
Eco88I	CYCGRG	1 cut(s)	984
Eco91I	GGTNACC	2 cut(s)	28, 2777
EcoICRI	GAGCTC	1 cut(s)	327
EcoO109I	RGGNCCY	3 cut(s)	53, 3081, 3308
EcoO65I	GGTNACC	2 cut(s)	28, 2777
EcoRI	GAATTC	1 cut(s)	3278
EcoRII	CCWGG	6 cut(s)	1705, 2235, 2506, 2656, 2824, 2864
EcoRV	GATATC	1 cut(s)	2652
EcoT14I	CCWWGG	1 cut(s)	2701
EcoT22I	ATGCAT	3 cut(s)	1669, 1834, 3010
EcoT38I	GRGCYC	2 cut(s)	212, 329
ErhI	CCWWGG	1 cut(s)	2701
Esp3I	CGTCTC	1 cut(s)	131
FalI	AAGNNNNNCTT	4 cut(s)	180, 212, 3266, 3298
FaqI	GGGAC	5 cut(s)	66, 1194, 2268, 3058, 3094
FauI	CCCGC	1 cut(s)	2274
FauNDI	CATATG	1 cut(s)	3214
FbaI	TGATCA	1 cut(s)	1245
FblI	GTMKAC	2 cut(s)	2474, 2861
FokI	GGATG	7 cut(s)	962, 1574, 2398, 2739, 3005, 3314, 3382
FriOI	GRGCYC	2 cut(s)	212, 329
GsaI	CCCAGC	5 cut(s)	208, 678, 995, 2979, 3081
GsuI	CTGGAG	4 cut(s)	1716, 1982, 2520, 2679
HaeIII	GGCC	9 cut(s)	924, 1290, 1552, 1896, 1947, 2142, 3032, 3267, 3309
HgaI	GACGC	6 cut(s)	99, 112, 152, 263, 286, 880
Hin1I	GRCGYC	3 cut(s)	110, 123, 297
HindIII	AAGCTT	4 cut(s)	791, 1307, 1337, 1688
Hpy166II	GTNNAC	8 cut(s)	580, 688, 730, 2092, 2475, 2862, 2918, 2945
Hpy8I	GTNNAC	8 cut(s)	580, 688, 730, 2092, 2475, 2862, 2918, 2945
Hpy99I	CGWCG	4 cut(s)	128, 257, 302, 1033
HpyCH4III	ACNGT	6 cut(s)	626, 1439, 2533, 2609, 2715, 2963
HpyCH4IV	ACGT	2 cut(s)	233, 3116
HpyF10VI	GCNNNNNNNGC	9 cut(s)	303, 448, 934, 988, 1296, 1624, 2589, 3442, 3469
HpySE526I	ACGT	2 cut(s)	233, 3116
Hsp92I	GRCGYC	3 cut(s)	110, 123, 297
KflI	GGGWCCC	1 cut(s)	53
Kpn2I	TCCGGA	1 cut(s)	239
KroI	GCCGGC	1 cut(s)	924
KroNI	GCCGGC	1 cut(s)	926
Ksp22I	TGATCA	1 cut(s)	1245
LmnI	GCTCC	9 cut(s)	332, 426, 698, 1087, 1348, 1697, 2589, 2630, 3487
Lsp1109I	GCAGC	6 cut(s)	1215, 1286, 2032, 2335, 3445, 3459
LweI	GCATC	8 cut(s)	155, 808, 1819, 1841, 2420, 2435, 3292, 3459
MabI	ACCWGGT	1 cut(s)	2235
MaeII	ACGT	2 cut(s)	233, 3116
MaeIII	GTNAC	8 cut(s)	28, 111, 224, 815, 2167, 2527, 2764, 2777
MfeI	CAATTG	3 cut(s)	742, 1091, 2400
MflI	RGATCY	7 cut(s)	660, 1170, 1465, 1517, 1957, 2083, 3196
MhlI	GDGCHC	5 cut(s)	212, 329, 1596, 2585, 2920
MlsI	TGGCCA	3 cut(s)	1947, 2142, 3032
MluNI	TGGCCA	3 cut(s)	1947, 2142, 3032
MlyI	GAGTC	7 cut(s)	176, 252, 638, 1070, 2670, 2728, 3358
Mox20I	TGGCCA	3 cut(s)	1947, 2142, 3032
Mph1103I	ATGCAT	3 cut(s)	1669, 1834, 3010
MroI	TCCGGA	1 cut(s)	239
MroNI	GCCGGC	1 cut(s)	924
MroXI	GAANNNNTTC	2 cut(s)	906, 1644
MscI	TGGCCA	3 cut(s)	1947, 2142, 3032
MseI	TTAA	8 cut(s)	474, 612, 1038, 1293, 1752, 1899, 2273, 3003
MslI	CAYNNNNRTG	5 cut(s)	1546, 2482, 2787, 2964, 3244
Msp20I	TGGCCA	3 cut(s)	1947, 2142, 3032
MspA1I	CMGCKG	2 cut(s)	67, 1398
MunI	CAATTG	3 cut(s)	742, 1091, 2400
Mva1269I	GAATGC	2 cut(s)	1542, 1667
MvaI	CCWGG	6 cut(s)	1707, 2237, 2508, 2658, 2826, 2866
MvnI	CGCG	2 cut(s)	257, 3390
MwoI	GCNNNNNNNGC	9 cut(s)	303, 448, 934, 988, 1296, 1624, 2589, 3442, 3469
NaeI	GCCGGC	1 cut(s)	926
NciI	CCSGG	4 cut(s)	51, 559, 683, 702
NdeI	CATATG	1 cut(s)	3214
NgoMIV	GCCGGC	1 cut(s)	924
NheI	GCTAGC	1 cut(s)	2600
NmeAIII	GCCGAG	1 cut(s)	900
NmuCI	GTSAC	5 cut(s)	28, 111, 224, 2167, 2764
NsiI	ATGCAT	3 cut(s)	1669, 1834, 3010
NspI	RCATGY	3 cut(s)	2039, 3249, 3360
NspV	TTCGAA	2 cut(s)	887, 1910
OliI	CACNNNNGTG	1 cut(s)	2482
PaeR7I	CTCGAG	1 cut(s)	984
PciI	ACATGT	1 cut(s)	3356
PcsI	WCGNNNNNNNCGW	1 cut(s)	3265
PctI	GAATGC	2 cut(s)	1542, 1667
PdiI	GCCGGC	1 cut(s)	926
PdmI	GAANNNNTTC	2 cut(s)	906, 1644
PflFI	GACNNNGTC	2 cut(s)	559, 647
PfoI	TCCNGGA	1 cut(s)	49
PleI	GAGTC	7 cut(s)	176, 251, 638, 1070, 2669, 2728, 3357
PmaCI	CACGTG	1 cut(s)	234
PmlI	CACGTG	1 cut(s)	234
PpsI	GAGTC	7 cut(s)	176, 251, 638, 1070, 2669, 2728, 3357
Ppu21I	YACGTR	1 cut(s)	234
PpuMI	RGGWCCY	2 cut(s)	53, 3081
PscI	ACATGT	1 cut(s)	3356
PsiI	TTATAA	1 cut(s)	2199
Psp124BI	GAGCTC	1 cut(s)	329
Psp5II	RGGWCCY	2 cut(s)	53, 3081
Psp6I	CCWGG	6 cut(s)	1705, 2235, 2506, 2656, 2824, 2864
PspCI	CACGTG	1 cut(s)	234
PspEI	GGTNACC	2 cut(s)	28, 2777
PspFI	CCCAGC	5 cut(s)	204, 674, 991, 2975, 3077
PspGI	CCWGG	6 cut(s)	1705, 2235, 2506, 2656, 2824, 2864
PspPI	GGNCC	7 cut(s)	53, 237, 1181, 1551, 2504, 3081, 3308
PspPPI	RGGWCCY	2 cut(s)	53, 3081
PstI	CTGCAG	3 cut(s)	860, 1620, 3292
PstNI	CAGNNNCTG	1 cut(s)	228
PsuI	RGATCY	7 cut(s)	660, 1170, 1465, 1517, 1957, 2083, 3196
PsyI	GACNNNGTC	2 cut(s)	559, 647
RsaI	GTAC	4 cut(s)	731, 1441, 2301, 2946
RsaNI	GTAC	4 cut(s)	730, 1440, 2300, 2945
RseI	CAYNNNNRTG	5 cut(s)	1546, 2482, 2787, 2964, 3244
SacI	GAGCTC	1 cut(s)	329
SaqAI	TTAA	8 cut(s)	474, 612, 1038, 1293, 1752, 1899, 2273, 3003
Sau96I	GGNCC	7 cut(s)	53, 237, 1181, 1551, 2504, 3081, 3308
ScaI	AGTACT	1 cut(s)	1441
SchI	GAGTC	7 cut(s)	176, 252, 638, 1070, 2670, 2728, 3358
SduI	GDGCHC	5 cut(s)	212, 329, 1596, 2585, 2920
SexAI	ACCWGGT	1 cut(s)	2235
SfaNI	GCATC	8 cut(s)	155, 808, 1819, 1841, 2420, 2435, 3292, 3459
SfcI	CTRYAG	3 cut(s)	856, 1616, 3288
Sfr274I	CTCGAG	1 cut(s)	984
SfuI	TTCGAA	2 cut(s)	887, 1910
SinI	GGWCC	5 cut(s)	53, 237, 1181, 2504, 3081
SlaI	CTCGAG	1 cut(s)	984
SmiMI	CAYNNNNRTG	5 cut(s)	1546, 2482, 2787, 2964, 3244
SspI	AATATT	1 cut(s)	2983
SstI	GAGCTC	1 cut(s)	329
StyI	CCWWGG	1 cut(s)	2701
TaaI	ACNGT	6 cut(s)	626, 1439, 2533, 2609, 2715, 2963
TaiI	ACGT	2 cut(s)	236, 3119
TaqII	GACCGA	2 cut(s)	202, 1198
TatI	WGTACW	3 cut(s)	729, 1439, 2299
TauI	GCSGC	5 cut(s)	67, 107, 110, 294, 297
Tru1I	TTAA	8 cut(s)	474, 612, 1038, 1293, 1752, 1899, 2273, 3003
Tru9I	TTAA	8 cut(s)	474, 612, 1038, 1293, 1752, 1899, 2273, 3003
TseFI	GTSAC	5 cut(s)	28, 111, 224, 2167, 2764
TseI	GCWGC	6 cut(s)	1203, 1299, 2020, 2348, 3433, 3472
Tsp45I	GTSAC	5 cut(s)	28, 111, 224, 2167, 2764
TspDTI	ATGAA	9 cut(s)	516, 818, 1118, 1301, 1518, 2205, 2708, 3201, 3426
TspGWI	ACGGA	6 cut(s)	155, 254, 324, 348, 685, 2494
Tth111I	GACNNNGTC	2 cut(s)	559, 647
VneI	GTGCAC	1 cut(s)	2916
VpaK11BI	GGWCC	5 cut(s)	53, 237, 1181, 2504, 3081
XceI	RCATGY	3 cut(s)	2039, 3249, 3360
XcmI	CCANNNNNNNNNTGG	3 cut(s)	64, 2150, 3065
XhoI	CTCGAG	1 cut(s)	984
XmiI	GTMKAC	2 cut(s)	2474, 2861
XmnI	GAANNNNTTC	2 cut(s)	906, 1644
ZrmI	AGTACT	1 cut(s)	1441
Zsp2I	ATGCAT	3 cut(s)	1669, 1834, 3010

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.