Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0010808

Physical Location & Seq

Genomic Coordinates Reverse (-)

76231215 .. 76234642

Gene Structure 3428 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G048670.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3282 bp

ATGGGTGTTCGATCTTCTTCCTCTCCGGTCACCAATTTCCTCTCTTTTCCGGGACCCACTTCGCCGCTGGTTCTGAAGCTCGTTTTGGTGCTGTGGGCGTGCGCGGCGGCGTCACAGAACGGCGTCGTTTCGGCGGAGACGGACGCATCGGTGCTGCTTCAGCTCAAAGGCTTAGTCTTGGACTCGGAAGGGTTGCTTTCGAGCTGGGGCTCGGTCAATTCCAGTCACTGCACCTGGTCAGGAGTCTTGTGCGACGCGAATTTCCGTGTCGTTTCGCTCAACATTACCGGCGCGGCGCCGGCGGAGGTAAATCGAAATCGAGCTCCTTTCCGTGCGTTGATTTTGTGGGAGGCTGGTGGGGAAGCTCCCTTTGTGATTGGGAAGCTCACTGAGCTAAAGGTCTTGTCTTTGCCCTTTAATGGTTTTGATGGTGAAATTCCTGTTGAAATTTGGGGCATGGAGAACCTAGAGGTCGTTGATCTTGAAGGGAATTCGATCACCGGGTCTTTGCCTTTTCGGGTGAACCCGAATTTGAGGGTTCTGAATCTTGGGTTTAATAAGATTCAGGGCGAGATACCGATCTTTGACTCTGTCAGCTTAGAGATCTTGAATTTAGCTGGGAACCGGGTGAACGGCTCCGTTCCGGGTTTCGTTGGCAGGTTGGCAGGGTTGTGGAAGCTTGAGCATCTTGATTTGTCTGGTAACTTCTTGGTTCATAAGATTCCGAGTAGTCTGGGGAACTGCAGTCGGCTGAGGACGCTGTTGTTGTATTCGAATATGTTGGAAGAAGGTATTCCGGCTGAACTCGGCCGGCTTCAGGCGCTTGAGGTGTTGGATGTGTCGAGGAATAGCTTGAGTGGTTCGTTGCCTCGAGAGCTGGGGAATTGTTCTCAGTTGTCTGTGCTTGTGCTTTCGTCGCTGTTTAATCCCCTTCCCCTTGTGCGAGCCAATTATACAGATGACTCGTTGTTGGAGCAGTTGAGTTCCATGAATGATGACTTCAATTACTTTCAAGGCTCAATGCCGAAGGAGATTACAAGCCTTCCAAAGCTGAAGATCTTGTGGGGACCGAGGGCAAGTATTGAGGGCAACTTTCCAAGTGATTGGGGTGCTTGTGAGAACTTGGAAATGATCAATTTGGCTCAGAATTTTTTTACTGGGGAAATTTCTAGTGGGCTTAACCGCTGCCAGAAGCTTCATTTTCTTGACCTAAGCACTAACAAGCTTACTGGGGAGCTTGTTCAGGTCCTTCAGGTTCCTTGTTCATTGCCTGAATATGCTAACAGTACTTGTCGTCCTGTTTCCTCTGTGGATCTTTCTTTCAAAGATGATGATTTTTCCTCGCCCTATGAAGCATTTTTTGGATCTAAAGCTCAAGCTGGAATGCCTTTGCAATGGCCCACAGAGGATGATGTTGTTGTAGTGATTCACAACTTTGGGCACAATAATTTTACCGGCACTCTGCAGTCATTGCCCATTGCACCCGAAAGGTTTCAGAAGAAAATTCTGTATGCATTCCTTGTTGGAGAAAACAAGCTTACTGGAGCATTTCCAGGTAATTTGTTTGGGAAGTGTCAGGGATTAGATTCACTGGTTGTTAATGTAAGCAACAACAGGTTAGATGGGAAGATTCCAACTGAAATTGGTAAAATGTGCAATTCTCTCAAGTTTTTAGATGCATCTCTGAATCGGATTGTGGGTTCCATTCCTGCTAGTTTTGGAGAATTGGTGTCTCTTGCTGGCCTTAACTTGAGTTCGAACAGGTTGCAAGGTCAAATTCCGACCACTCTTGGCCAGATAAGGGATCTGGAGCGTCTTTCTTTGTCTGGTAATAACCTTACAGGTGTTATTCCTGCTAGTTTGGGGCAGGTTTACTCCTTGCATGTGCTTGAGCTTTCTCGGAACTCTCTCACTGGTGAGATACCAAACGATCTTGTAAACTTGCGAAACTTGAGAGTTCTTCTGCTGGACAAAAATAAACTTTCTGGCCAGATTCCATCTGGTTTGGCAAATGTGACCACGCTCTCAGCCTTCAATGTGTCTTACAATAACTTCTCTGGTTCATTGCCTTTGAATAATAACCTGGTGAATTGCAATACTGCTATTGGAAACCCCCATTTAAGTTCCTGCCCCTCACTTTCTCAAGTACAGCCAGCTAATACTCAAGGAAGAGTTGGCGATTCAGAACCTTATGCTGCTTCACCATTGGTAGGAACTCAGAAGAGTGCTGGTAGCGGTTTCAATTCAATTGAAATAGCATCCATTACATCTGCATCTGCCATTGTTTTAGTGCTTATAGCTCTTGTTGTTCTATTCTTGTATACGCGGAAGTGGAATCGGAAGTCTGGAGGTCCAGGCTCTACTAGGAAGGAAGTTACTGTTTTCACAAACATTGGGGTTCCACTGACCTTTGATAGTGTTGTTCGGGGCACGGGGAGCTTCAATGCTAGCAACTGTATTGGGAATGGAGGTTTTGGAGCAACTTACAAGGCAGAGATATCACCTGGAGTCCTAGTGGCTATAAAACGGCTTGCTGTTGGAAGGTTCCAAGGAGTGCAACAGTTTCATGCAGAAATCAAAACACTGGGGAGGCTTCGACATCCAAATCTGGTCACTTTACTAGGTTACCATGCCAGTGAAACAGAGATGTTCCTAATATATAATTATTTTCCTGGTGGTAATCTGGAAAAATTTATTCAGGAAAGGTCTACCAGGGCAGTGGATTGGAAGATACTTCACAAGATTGCTTTGGACATAGCCCGTGCACTTGCCTACCTACATGATCAGTGTGTACCTCGTGTCCTTCACCGTGATGTCAAGCCCAGCAATATTTTGTTGGATGATGATTTTAATGCATATCTATCAGACTTTGGTTTGGCCAGACTTCTTGGGACTTCAGAAACCCATGCTACTACTGGTGTAGCAGGGACCTTTGGATATGTTGCCCCAGAATATGCAATGACGTGTCGTGTTTCAGATAAGTCTGATGTGTATAGTTATGGTGTAGTGCTTCTTGAGTTGCTGTCTGACAAGAAAGTGTTGGATCCTTCCTTTTCTTCATATGGAAATGGATTCAACATCGTTGCATGGGCATGTATGCTTCTTCGACAAGGCCGTGCAAAGGAATTCTTTTCTGCAGGGTTGTGGGATGCAGGCCCCCATGATGATTTGGTTGAAGTTCTACACTTGGCAGTGGTATGTACGGTTGATTCTCTCTCGACTAGGCCTACTATGAGGCAAGTCGTTCGACGGCTAAAGCAACTTCAACCCCCCTCATGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000578 GO:0001101 GO:0003002 GO:0003006 GO:0005575 GO:0005623 GO:0005886 GO:0006725 GO:0006950 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009555 GO:0009628 GO:0009698 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009808 GO:0009846 GO:0009856 GO:0009880 GO:0009888 GO:0009908 GO:0009942 GO:0009945 GO:0009956 GO:0009987 GO:0010035 GO:0010073 GO:0010152 GO:0010154 GO:0016020 GO:0016043 GO:0019748 GO:0021700 GO:0022414 GO:0022607 GO:0032501 GO:0032502 GO:0042221 GO:0043933 GO:0044085 GO:0044237 GO:0044464 GO:0044706 GO:0048229 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048507 GO:0048508 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051259 GO:0051260 GO:0051704 GO:0061458 GO:0065003 GO:0071704 GO:0071840 GO:0071944 GO:0090567 GO:0099402 GO:1901360 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1093

Amino Acids

118.33

Weight (kDa)

6.32

Isoelectric Point (pI)

35.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	46 - 85	9.3e-09	Leucine rich repeat N-terminal domain
LRR_14	PF23598	243 - 354	1.6e-06	Leucine-rich repeat region
LRR_8	PF13855	602 - 661	2e-08	Leucine rich repeat
Pkinase	PF00069	816 - 1015	1e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	817 - 1085	6.4e-40	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0010808)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02130 AT3G02130
fragaria_vesca	FvH4_6g46830
malus_domestica	MD09G1067100.v1.1 MD17G1060000.v1.1 MD17G1060100.v1.1
prunus_persica	Prupe.3G254600_v2.0.a1
pyrus_communis	pycom111g05640 pycom17g05970 pycom17g05980
rosa_chinensis	RchiOBHm_Chr2g0165821
rosa_laevigata	RLG00000021558
rosa_multiflora	Rmu_sc0003227.1_g000004
rosa_roxburghii	Rroxscaffold_2G00085480
rosa_rugosa	Rorug02G0518000
rosa_samantha	Rh2AG583700 Rh2BG595700 Rh2CG566600 Rh2DG605500
rosa_wichuraiana	Rw2G048670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	648, 1858
AccB1I	GGYRCC	1 cut(s)	295
AccI	GTMKAC	2 cut(s)	2321, 2708
AccII	CGCG	4 cut(s)	104, 257, 293, 2326
AciI	CCGC	9 cut(s)	65, 104, 107, 134, 293, 302, 1183, 2235, 2326
AclWI	GGATC	5 cut(s)	1320, 1372, 1812, 3038, 3051
AcoI	YGGCCR	4 cut(s)	808, 1792, 1987, 2877
AcuI	CTGAAG	6 cut(s)	95, 143, 800, 1073, 1235, 2880
AcyI	GRCGYC	3 cut(s)	110, 123, 296
AfaI	GTAC	4 cut(s)	1288, 2148, 2793, 3202
AfiI	CCNNNNNNNGG	5 cut(s)	419, 817, 1801, 2210, 3121
AflIII	ACRYGT	1 cut(s)	2963
AjiI	CACGTC	1 cut(s)	2964
AjnI	CCWGG	7 cut(s)	233, 1552, 2082, 2353, 2503, 2671, 2711
AjuI	GAANNNNNNNTTGG	4 cut(s)	68, 100, 1344, 1376
AloI	GAACNNNNNNTCC	2 cut(s)	1684, 1716
Alw21I	GWGCWC	2 cut(s)	325, 2767
Alw26I	GTCTC	2 cut(s)	131, 1737
Alw44I	GTGCAC	1 cut(s)	2763
AlwI	GGATC	5 cut(s)	1320, 1372, 1812, 3038, 3051
AlwNI	CAGNNNCTG	1 cut(s)	228
Ama87I	CYCGRG	1 cut(s)	870
AoxI	GGCC	9 cut(s)	808, 1397, 1741, 1792, 1987, 2877, 3112, 3154, 3224
ApaLI	GTGCAC	1 cut(s)	2763
ApeKI	GCWGC	3 cut(s)	154, 1185, 2195
ArsI	GACNNNNNNTTYG	2 cut(s)	159, 191
Asp700I	GAANNNNTTC	2 cut(s)	792, 1491
AspLEI	GCGC	4 cut(s)	104, 293, 298, 823
AspS9I	GGNCC	7 cut(s)	53, 1067, 1246, 1398, 2351, 2928, 3155
AsuC2I	CCSGG	4 cut(s)	51, 502, 626, 645
AsuII	TTCGAA	2 cut(s)	773, 1757
AsuNHI	GCTAGC	1 cut(s)	2447
AvaI	CYCGRG	1 cut(s)	870
AvaII	GGWCC	5 cut(s)	53, 1067, 1246, 2351, 2928
BaeGI	GKGCMC	3 cut(s)	1443, 2432, 2767
BalI	TGGCCA	3 cut(s)	1794, 1989, 2879
BamHI	GGATCC	1 cut(s)	3043
BanI	GGYRCC	1 cut(s)	295
BanII	GRGCYC	2 cut(s)	212, 325
BauI	CACGAG	1 cut(s)	2796
Bbv12I	GWGCWC	2 cut(s)	325, 2767
BbvCI	CCTCAGC	1 cut(s)	752
BbvI	GCAGC	3 cut(s)	141, 1172, 2182
BccI	CCATC	3 cut(s)	422, 1616, 2005
BceAI	ACGGC	5 cut(s)	136, 649, 2543, 3099, 3266
BcgI	CGANNNNNNTGC	4 cut(s)	1747, 1781, 3227, 3261
BciT130I	CCWGG	7 cut(s)	235, 1554, 2084, 2355, 2505, 2673, 2713
BclI	TGATCA	2 cut(s)	1131, 2782
BcnI	CCSGG	4 cut(s)	51, 502, 626, 645
BcoDI	GTCTC	2 cut(s)	131, 1737
BfaI	CTAG	9 cut(s)	467, 1170, 1713, 1857, 2364, 2448, 2513, 2621, 3222
BfmI	CTRYAG	3 cut(s)	742, 1463, 3135
BfoI	RGCGCY	2 cut(s)	299, 824
BfuAI	ACCTGC	2 cut(s)	648, 1858
BglII	AGATCT	2 cut(s)	603, 1056
BisI	GCNGC	7 cut(s)	65, 105, 108, 155, 294, 1186, 2196
BlsI	GCNGC	7 cut(s)	66, 106, 109, 156, 295, 1187, 2197
BmcAI	AGTACT	1 cut(s)	1288
Bme18I	GGWCC	5 cut(s)	53, 1067, 1246, 2351, 2928
BmeT110I	CYCGRG	1 cut(s)	870
BmgBI	CACGTC	1 cut(s)	2964
BmgT120I	GGNCC	7 cut(s)	53, 1067, 1246, 1398, 2351, 2928, 3155
BmrI	ACTGGG	3 cut(s)	1167, 1239, 2594
BmsI	GCATC	7 cut(s)	155, 694, 1666, 1688, 2267, 2282, 3139
BmtI	GCTAGC	1 cut(s)	2451
BmuI	ACTGGG	3 cut(s)	1167, 1239, 2594
BpmI	CTGGAG	4 cut(s)	1563, 1829, 2367, 2526
Bpu10I	CCTNAGC	2 cut(s)	752, 1211
Bpu14I	TTCGAA	2 cut(s)	773, 1757
BpuMI	CCSGG	4 cut(s)	51, 502, 626, 645
BsaBI	GATNNNNATC	1 cut(s)	578
BsaHI	GRCGYC	3 cut(s)	110, 123, 296
BsaJI	CCNNGG	3 cut(s)	1070, 2548, 2712
BsaWI	WCCGGW	1 cut(s)	25
BsaXI	ACNNNNNCTCC	4 cut(s)	1713, 1743, 2499, 2529
Bsc4I	CCNNNNNNNGG	5 cut(s)	419, 817, 1801, 2210, 3121
Bse118I	RCCGGY	4 cut(s)	287, 298, 810, 1454
Bse1I	ACTGG	9 cut(s)	222, 1162, 1234, 1546, 1596, 1918, 2589, 2634, 2920
Bse3DI	GCAATG	6 cut(s)	1265, 1400, 1469, 1476, 2063, 2964
Bse8I	GATNNNNATC	1 cut(s)	578
BseBI	CCWGG	7 cut(s)	235, 1554, 2084, 2355, 2505, 2673, 2713
BseDI	CCNNGG	3 cut(s)	1070, 2548, 2712
BseGI	GGATG	6 cut(s)	841, 1414, 2258, 2599, 2845, 3154
BseJI	GATNNNNATC	1 cut(s)	578
BseLI	CCNNNNNNNGG	5 cut(s)	419, 817, 1801, 2210, 3121
BseMI	GCAATG	6 cut(s)	1265, 1400, 1469, 1476, 2063, 2964
BseMII	CTCAG	6 cut(s)	381, 743, 905, 1157, 2040, 2231
BseNI	ACTGG	9 cut(s)	222, 1162, 1234, 1546, 1596, 1918, 2589, 2634, 2920
BseSI	GKGCMC	3 cut(s)	1443, 2432, 2767
BseX3I	CGGCCG	1 cut(s)	808
BseXI	GCAGC	3 cut(s)	141, 1172, 2182
BseYI	CCCAGC	4 cut(s)	204, 617, 877, 2822
BsgI	GTGCAG	1 cut(s)	214
Bsh1236I	CGCG	4 cut(s)	104, 257, 293, 2326
Bsh1285I	CGRYCG	1 cut(s)	811
BshFI	GGCC	9 cut(s)	810, 1399, 1743, 1794, 1989, 2879, 3114, 3156, 3226
BshNI	GGYRCC	1 cut(s)	295
BsiEI	CGRYCG	1 cut(s)	811
BsiHKAI	GWGCWC	2 cut(s)	325, 2767
BsiHKCI	CYCGRG	1 cut(s)	870
BslFI	GGGAC	4 cut(s)	66, 1080, 2905, 2941
BslI	CCNNNNNNNGG	5 cut(s)	419, 817, 1801, 2210, 3121
BsmAI	GTCTC	2 cut(s)	131, 1737
BsmBI	CGTCTC	1 cut(s)	131
BsmFI	GGGAC	4 cut(s)	66, 1080, 2905, 2941
BsmI	GAATGC	2 cut(s)	1389, 1514
BsnI	GGCC	9 cut(s)	810, 1399, 1743, 1794, 1989, 2879, 3114, 3156, 3226
BsoBI	CYCGRG	1 cut(s)	870
Bsp119I	TTCGAA	2 cut(s)	773, 1757
Bsp1286I	GDGCHC	5 cut(s)	212, 325, 1443, 2432, 2767
BspACI	CCGC	9 cut(s)	65, 104, 107, 134, 293, 302, 1183, 2235, 2326
BspANI	GGCC	9 cut(s)	810, 1399, 1743, 1794, 1989, 2879, 3114, 3156, 3226
BspCNI	CTCAG	6 cut(s)	382, 744, 904, 1156, 2039, 2230
BspFNI	CGCG	4 cut(s)	104, 257, 293, 2326
BspMAI	CTGCAG	3 cut(s)	746, 1467, 3139
BspMI	ACCTGC	2 cut(s)	648, 1858
BspOI	GCTAGC	1 cut(s)	2451
BspPI	GGATC	5 cut(s)	1320, 1372, 1812, 3038, 3051
BspT104I	TTCGAA	2 cut(s)	773, 1757
BspT107I	GGYRCC	1 cut(s)	295
BsrDI	GCAATG	6 cut(s)	1265, 1400, 1469, 1476, 2063, 2964
BsrFI	RCCGGY	4 cut(s)	287, 298, 810, 1454
BsrI	ACTGG	9 cut(s)	222, 1162, 1234, 1546, 1596, 1918, 2589, 2634, 2920
BssAI	RCCGGY	4 cut(s)	287, 298, 810, 1454
BssECI	CCNNGG	3 cut(s)	1070, 2548, 2712
BssNAI	GTATAC	1 cut(s)	2322
BssNI	GRCGYC	3 cut(s)	110, 123, 296
BssSI	CACGAG	1 cut(s)	2796
BssT1I	CCWWGG	1 cut(s)	2548
Bst1107I	GTATAC	1 cut(s)	2322
Bst2BI	CACGAG	1 cut(s)	2796
Bst2UI	CCWGG	7 cut(s)	235, 1554, 2084, 2355, 2505, 2673, 2713
Bst4CI	ACNGT	6 cut(s)	1286, 2380, 2456, 2562, 2810, 3205
Bst6I	CTCTTC	2 cut(s)	2164, 2216
BstACI	GRCGYC	3 cut(s)	110, 123, 296
BstAPI	GCANNNNNTGC	1 cut(s)	1471
BstBI	TTCGAA	2 cut(s)	773, 1757
BstC8I	GCNNGC	9 cut(s)	100, 300, 812, 945, 1741, 2154, 2449, 2532, 3154
BstDEI	CTNAG	9 cut(s)	172, 390, 598, 752, 891, 1143, 1211, 2026, 2217
BstEII	GGTNACC	2 cut(s)	28, 2624
BstF5I	GGATG	6 cut(s)	841, 1414, 2258, 2599, 2845, 3154
BstFNI	CGCG	4 cut(s)	104, 257, 293, 2326
BstH2I	RGCGCY	2 cut(s)	299, 824
BstHHI	GCGC	4 cut(s)	104, 293, 298, 823
BstMAI	GTCTC	2 cut(s)	131, 1737
BstMCI	CGRYCG	1 cut(s)	811
BstNI	CCWGG	7 cut(s)	235, 1554, 2084, 2355, 2505, 2673, 2713
BstNSI	RCATGY	2 cut(s)	1886, 3096
BstPI	GGTNACC	2 cut(s)	28, 2624
BstSFI	CTRYAG	3 cut(s)	742, 1463, 3135
BstSLI	GKGCMC	3 cut(s)	1443, 2432, 2767
BstUI	CGCG	4 cut(s)	104, 257, 293, 2326
BstV1I	GCAGC	3 cut(s)	141, 1172, 2182
BstX2I	RGATCY	6 cut(s)	603, 1056, 1312, 1364, 1804, 3043
BstXI	CCANNNNNNTGG	2 cut(s)	1104, 2719
BstYI	RGATCY	6 cut(s)	603, 1056, 1312, 1364, 1804, 3043
BstZ17I	GTATAC	1 cut(s)	2322
BstZI	CGGCCG	1 cut(s)	808
BsuRI	GGCC	9 cut(s)	810, 1399, 1743, 1794, 1989, 2879, 3114, 3156, 3226
BtrI	CACGTC	1 cut(s)	2964
BtsCI	GGATG	6 cut(s)	841, 1414, 2258, 2599, 2845, 3154
BtsI	GCAGTG	3 cut(s)	226, 2724, 3198
BveI	ACCTGC	2 cut(s)	648, 1858
Cac8I	GCNNGC	9 cut(s)	100, 300, 812, 945, 1741, 2154, 2449, 2532, 3154
CaiI	CAGNNNCTG	1 cut(s)	228
CfoI	GCGC	4 cut(s)	104, 293, 298, 823
Cfr10I	RCCGGY	4 cut(s)	287, 298, 810, 1454
Cfr13I	GGNCC	7 cut(s)	53, 1067, 1246, 1398, 2351, 2928, 3155
CseI	GACGC	6 cut(s)	99, 112, 152, 263, 766, 1802
CsiI	ACCWGGT	2 cut(s)	233, 2082
Csp6I	GTAC	4 cut(s)	1287, 2147, 2792, 3201
CviQI	GTAC	4 cut(s)	1287, 2147, 2792, 3201
DdeI	CTNAG	9 cut(s)	172, 390, 598, 752, 891, 1143, 1211, 2026, 2217
DinI	GGCGCC	1 cut(s)	297
EaeI	YGGCCR	4 cut(s)	808, 1792, 1987, 2877
EagI	CGGCCG	1 cut(s)	808
Eam1104I	CTCTTC	2 cut(s)	2164, 2216
EarI	CTCTTC	2 cut(s)	2164, 2216
EciI	GGCGGA	2 cut(s)	149, 317
Ecl136II	GAGCTC	1 cut(s)	323
EclXI	CGGCCG	1 cut(s)	808
Eco130I	CCWWGG	1 cut(s)	2548
Eco147I	AGGCCT	1 cut(s)	3226
Eco24I	GRGCYC	2 cut(s)	212, 325
Eco32I	GATATC	1 cut(s)	2499
Eco47I	GGWCC	5 cut(s)	53, 1067, 1246, 2351, 2928
Eco52I	CGGCCG	1 cut(s)	808
Eco53kI	GAGCTC	1 cut(s)	323
Eco57I	CTGAAG	6 cut(s)	95, 143, 800, 1073, 1235, 2880
Eco88I	CYCGRG	1 cut(s)	870
Eco91I	GGTNACC	2 cut(s)	28, 2624
EcoICRI	GAGCTC	1 cut(s)	323
EcoO109I	RGGNCCY	4 cut(s)	53, 1246, 2928, 3155
EcoO65I	GGTNACC	2 cut(s)	28, 2624
EcoRI	GAATTC	2 cut(s)	490, 3125
EcoRII	CCWGG	7 cut(s)	233, 1552, 2082, 2353, 2503, 2671, 2711
EcoRV	GATATC	1 cut(s)	2499
EcoT14I	CCWWGG	1 cut(s)	2548
EcoT22I	ATGCAT	3 cut(s)	1516, 1681, 2857
EcoT38I	GRGCYC	2 cut(s)	212, 325
EgeI	GGCGCC	1 cut(s)	297
EheI	GGCGCC	1 cut(s)	297
ErhI	CCWWGG	1 cut(s)	2548
Esp3I	CGTCTC	1 cut(s)	131
FalI	AAGNNNNNCTT	4 cut(s)	180, 212, 3113, 3145
FaqI	GGGAC	4 cut(s)	66, 1080, 2905, 2941
FauNDI	CATATG	1 cut(s)	3061
FbaI	TGATCA	2 cut(s)	1131, 2782
FblI	GTMKAC	2 cut(s)	2321, 2708
Fnu4HI	GCNGC	7 cut(s)	65, 105, 108, 155, 294, 1186, 2196
FokI	GGATG	6 cut(s)	848, 1421, 2245, 2586, 2852, 3161
FriOI	GRGCYC	2 cut(s)	212, 325
Fsp4HI	GCNGC	7 cut(s)	65, 105, 108, 155, 294, 1186, 2196
FspBI	CTAG	9 cut(s)	467, 1170, 1713, 1857, 2364, 2448, 2513, 2621, 3222
GlaI	GCGC	4 cut(s)	103, 292, 297, 822
GluI	GCNGC	7 cut(s)	65, 105, 108, 155, 294, 1186, 2196
GsaI	CCCAGC	4 cut(s)	208, 621, 881, 2826
GsuI	CTGGAG	4 cut(s)	1563, 1829, 2367, 2526
HaeII	RGCGCY	2 cut(s)	299, 824
HaeIII	GGCC	9 cut(s)	810, 1399, 1743, 1794, 1989, 2879, 3114, 3156, 3226
HgaI	GACGC	6 cut(s)	99, 112, 152, 263, 766, 1802
HhaI	GCGC	4 cut(s)	104, 293, 298, 823
Hin1I	GRCGYC	3 cut(s)	110, 123, 296
Hin6I	GCGC	4 cut(s)	102, 291, 296, 821
HinP1I	GCGC	4 cut(s)	102, 291, 296, 821
HindIII	AAGCTT	4 cut(s)	677, 1193, 1223, 1535
Hpy166II	GTNNAC	8 cut(s)	523, 631, 1873, 1939, 2322, 2709, 2765, 2792
Hpy8I	GTNNAC	8 cut(s)	523, 631, 1873, 1939, 2322, 2709, 2765, 2792
Hpy99I	CGWCG	4 cut(s)	128, 257, 919, 3252
HpyCH4III	ACNGT	6 cut(s)	1286, 2380, 2456, 2562, 2810, 3205
HpyCH4IV	ACGT	1 cut(s)	2963
HpyF3I	CTNAG	9 cut(s)	172, 390, 598, 752, 891, 1143, 1211, 2026, 2217
HpySE526I	ACGT	1 cut(s)	2963
Hsp92I	GRCGYC	3 cut(s)	110, 123, 296
HspAI	GCGC	4 cut(s)	102, 291, 296, 821
KasI	GGCGCC	1 cut(s)	295
KflI	GGGWCCC	1 cut(s)	53
KroI	GCCGGC	2 cut(s)	298, 810
KroNI	GCCGGC	2 cut(s)	300, 812
Ksp22I	TGATCA	2 cut(s)	1131, 2782
LmnI	GCTCC	9 cut(s)	328, 370, 641, 973, 1234, 1544, 1810, 2436, 2477
Lsp1109I	GCAGC	3 cut(s)	141, 1172, 2182
LweI	GCATC	7 cut(s)	155, 694, 1666, 1688, 2267, 2282, 3139
MabI	ACCWGGT	2 cut(s)	233, 2082
MaeI	CTAG	9 cut(s)	467, 1170, 1713, 1857, 2364, 2448, 2513, 2621, 3222
MaeII	ACGT	1 cut(s)	2963
MaeIII	GTNAC	8 cut(s)	28, 111, 224, 701, 2014, 2374, 2611, 2624
MfeI	CAATTG	1 cut(s)	2247
MflI	RGATCY	6 cut(s)	603, 1056, 1312, 1364, 1804, 3043
MhlI	GDGCHC	5 cut(s)	212, 325, 1443, 2432, 2767
MlsI	TGGCCA	3 cut(s)	1794, 1989, 2879
MluNI	TGGCCA	3 cut(s)	1794, 1989, 2879
Mly113I	GGCGCC	1 cut(s)	296
MlyI	GAGTC	5 cut(s)	176, 252, 581, 956, 2517
MmeI	TCCRAC	9 cut(s)	762, 813, 951, 1504, 1658, 1805, 2518, 2817, 3021
Mox20I	TGGCCA	3 cut(s)	1794, 1989, 2879
Mph1103I	ATGCAT	3 cut(s)	1516, 1681, 2857
MreI	CGCCGGCG	1 cut(s)	298
MroNI	GCCGGC	2 cut(s)	298, 810
MroXI	GAANNNNTTC	2 cut(s)	792, 1491
MscI	TGGCCA	3 cut(s)	1794, 1989, 2879
MseI	TTAA	8 cut(s)	417, 555, 924, 1179, 1599, 1746, 2120, 2850
MslI	CAYNNNNRTG	3 cut(s)	2634, 2811, 3091
Msp20I	TGGCCA	3 cut(s)	1794, 1989, 2879
MspA1I	CMGCKG	2 cut(s)	67, 1185
MteI	GCGCNGCGC	1 cut(s)	294
MunI	CAATTG	1 cut(s)	2247
Mva1269I	GAATGC	2 cut(s)	1389, 1514
MvaI	CCWGG	7 cut(s)	235, 1554, 2084, 2355, 2505, 2673, 2713
MvnI	CGCG	4 cut(s)	104, 257, 293, 2326
NaeI	GCCGGC	2 cut(s)	300, 812
NarI	GGCGCC	1 cut(s)	296
NciI	CCSGG	4 cut(s)	51, 502, 626, 645
NdeI	CATATG	1 cut(s)	3061
NgoMIV	GCCGGC	2 cut(s)	298, 810
NheI	GCTAGC	1 cut(s)	2447
NmeAIII	GCCGAG	1 cut(s)	786
NmuCI	GTSAC	5 cut(s)	28, 111, 224, 2014, 2611
NsiI	ATGCAT	3 cut(s)	1516, 1681, 2857
NspI	RCATGY	2 cut(s)	1886, 3096
NspV	TTCGAA	2 cut(s)	773, 1757
PaeR7I	CTCGAG	1 cut(s)	870
PceI	AGGCCT	1 cut(s)	3226
PcsI	WCGNNNNNNNCGW	1 cut(s)	3112
PctI	GAATGC	2 cut(s)	1389, 1514
PdiI	GCCGGC	2 cut(s)	300, 812
PdmI	GAANNNNTTC	2 cut(s)	792, 1491
PflFI	GACNNNGTC	1 cut(s)	590
PfoI	TCCNGGA	1 cut(s)	49
PkrI	GCNGC	7 cut(s)	66, 106, 109, 156, 295, 1187, 2197
PleI	GAGTC	5 cut(s)	176, 251, 581, 956, 2516
PluTI	GGCGCC	1 cut(s)	299
PpsI	GAGTC	5 cut(s)	176, 251, 581, 956, 2516
PpuMI	RGGWCCY	3 cut(s)	53, 1246, 2928
Psp124BI	GAGCTC	1 cut(s)	325
Psp5II	RGGWCCY	3 cut(s)	53, 1246, 2928
Psp6I	CCWGG	7 cut(s)	233, 1552, 2082, 2353, 2503, 2671, 2711
PspEI	GGTNACC	2 cut(s)	28, 2624
PspFI	CCCAGC	4 cut(s)	204, 617, 877, 2822
PspGI	CCWGG	7 cut(s)	233, 1552, 2082, 2353, 2503, 2671, 2711
PspPI	GGNCC	7 cut(s)	53, 1067, 1246, 1398, 2351, 2928, 3155
PspPPI	RGGWCCY	3 cut(s)	53, 1246, 2928
PstI	CTGCAG	3 cut(s)	746, 1467, 3139
PstNI	CAGNNNCTG	1 cut(s)	228
PsuI	RGATCY	6 cut(s)	603, 1056, 1312, 1364, 1804, 3043
PsyI	GACNNNGTC	1 cut(s)	590
RsaI	GTAC	4 cut(s)	1288, 2148, 2793, 3202
RsaNI	GTAC	4 cut(s)	1287, 2147, 2792, 3201
RseI	CAYNNNNRTG	3 cut(s)	2634, 2811, 3091
SacI	GAGCTC	1 cut(s)	325
SaqAI	TTAA	8 cut(s)	417, 555, 924, 1179, 1599, 1746, 2120, 2850
SatI	GCNGC	7 cut(s)	65, 105, 108, 155, 294, 1186, 2196
Sau96I	GGNCC	7 cut(s)	53, 1067, 1246, 1398, 2351, 2928, 3155
ScaI	AGTACT	1 cut(s)	1288
SchI	GAGTC	5 cut(s)	176, 252, 581, 956, 2517
SduI	GDGCHC	5 cut(s)	212, 325, 1443, 2432, 2767
SexAI	ACCWGGT	2 cut(s)	233, 2082
SfaNI	GCATC	7 cut(s)	155, 694, 1666, 1688, 2267, 2282, 3139
SfcI	CTRYAG	3 cut(s)	742, 1463, 3135
SfoI	GGCGCC	1 cut(s)	297
Sfr274I	CTCGAG	1 cut(s)	870
SfuI	TTCGAA	2 cut(s)	773, 1757
SgrAI	CRCCGGYG	1 cut(s)	298
SinI	GGWCC	5 cut(s)	53, 1067, 1246, 2351, 2928
SlaI	CTCGAG	1 cut(s)	870
SmiMI	CAYNNNNRTG	3 cut(s)	2634, 2811, 3091
SseBI	AGGCCT	1 cut(s)	3226
SsiI	CCGC	9 cut(s)	65, 104, 107, 134, 293, 302, 1183, 2235, 2326
SspDI	GGCGCC	1 cut(s)	295
SspI	AATATT	1 cut(s)	2830
SspMI	CTAG	9 cut(s)	467, 1170, 1713, 1857, 2364, 2448, 2513, 2621, 3222
SstI	GAGCTC	1 cut(s)	325
StuI	AGGCCT	1 cut(s)	3226
StyI	CCWWGG	1 cut(s)	2548
TaaI	ACNGT	6 cut(s)	1286, 2380, 2456, 2562, 2810, 3205
TaiI	ACGT	1 cut(s)	2966
TaqII	GACCGA	2 cut(s)	202, 1084
TatI	WGTACW	2 cut(s)	1286, 2146
TauI	GCSGC	4 cut(s)	67, 107, 110, 296
Tru1I	TTAA	8 cut(s)	417, 555, 924, 1179, 1599, 1746, 2120, 2850
Tru9I	TTAA	8 cut(s)	417, 555, 924, 1179, 1599, 1746, 2120, 2850
TseFI	GTSAC	5 cut(s)	28, 111, 224, 2014, 2611
TseI	GCWGC	3 cut(s)	154, 1185, 2195
Tsp45I	GTSAC	5 cut(s)	28, 111, 224, 2014, 2611
TspDTI	ATGAA	8 cut(s)	704, 1004, 1187, 1254, 1365, 2052, 2555, 3048
TspGWI	ACGGA	4 cut(s)	155, 254, 320, 628
Tth111I	GACNNNGTC	1 cut(s)	590
VneI	GTGCAC	1 cut(s)	2763
VpaK11BI	GGWCC	5 cut(s)	53, 1067, 1246, 2351, 2928
XceI	RCATGY	2 cut(s)	1886, 3096
XcmI	CCANNNNNNNNNTGG	3 cut(s)	64, 1997, 2912
XhoI	CTCGAG	1 cut(s)	870
XmiI	GTMKAC	2 cut(s)	2321, 2708
XmnI	GAANNNNTTC	2 cut(s)	792, 1491
XspI	CTAG	9 cut(s)	467, 1170, 1713, 1857, 2364, 2448, 2513, 2621, 3222
ZrmI	AGTACT	1 cut(s)	1288
Zsp2I	ATGCAT	3 cut(s)	1516, 1681, 2857

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.