Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0010808

Physical Location & Seq

Genomic Coordinates Forward (+)

7766368 .. 7771344

Gene Structure 4977 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00085480.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3579 bp

ATGGGTGTTCGATCTTCTTCCTCTCCGGTCACCAATTTCCTGTCTTTTCCGGGACCCACTTCGCCGCTGGTTCTGCAGCTCGTTTTGGTGCTGTGGGTGTGCGCGGCGGCGTCCCAGAACGGCGTCGTTTCGGCGGAGACGGACGCATCGGTGCTGCTTCAGCTCAAAGACTCAGTCTTGGACTCGGAAGGGTTGCTTTCGAGCTGGGGCTCGGTCAATTCCAGTCACTGCACCTGGTCCGGAGTCTTGTGCGACGCGAATTTCCGTGTCGTTTCACTCAACATTACCGGCGCCGGCGCCGGCGGAGGTAAATCGAAATCGAGCTCCTTTCCGTGCGTTGATTTTGGTCAGTTTCCGTTTTATGGGTTGGGGATTAGGAGGAGTTGTGTGGGTGGTAGTGGGAGGTTGGTGGGGAAGCTCCCTTCTGTGATTGGGAAGCTCACTGAGCTAAAGGTCTTGTCTTTGCCCTTTAATGGTTTTGATGGTGAAATTCCTGTTCAAATTTGGGGCATGGAGAACCTAGAGGTCCTTGATCTTGAAGGAAATTCGATGACCGGGTCTTTGCCGTTTCGGGTGAACCCGAATTTGAGGGTTCTGAATCTTGGGTTTAATAAGATTCACGGTGAGATACCGATCTTTGACTCTGTCAGCTTAGAGATCTTGAACTTAGCTGGGAACCGGGTGAACGGCTCCGTTCCGGGTTTCGTTGGCAGGTTGAAAGGGGTGTACTTGTCATACAATTGGCTCAGTGGGGATATTCCTAGTGAGATTGGAGATGGTTGTGGGAAGCTTGAGCATCTTGATTTGTCTGGTAACTTCTTGGTTCATAAGATTCCGAGTAGTCTGGGGAATTGCAGTCGGCTGAGGACGCTGTTGTTGTATTCGAATATGTTGGAAGAAGGCATTCCGGCTGAACTCGGCCGGCTTCAGCTGCTTGAGGTGTTGGATGTGTCGAGGAATAGCCTGAGCGGTTCGTTGCCTCGAGAGCTGGGGACTTGTTCTCAGTTGTCTGTGCTTGTGCTTTCGTCGCTGTTTAATCCCCTTCCCCTTGTGCGAGCCAATTATACAGATGACTCGTTGTTGGAGCAGTTGAGTTCCATGAATGACGACTTCAATTACTTTCAAGGCTCAATGCCGAAGGAGATTACAAGCCTTCCAAAGCTGAAGATCTTGTGGGGACCGAGAGCAAGTATTGAGGGCAGCTTTCCGAGTGATTGGGGCGCTTGTGAGAACTTGGAAATGATCAATTTGGCTCAGAATTTTTTTACTGGGGGAATTTCTAGCGGGCTTAACCGCTGCCAGAAGCTTCATTTTCTTGACCTAAGCACTAACAAGCTTACTGGGGAGCTTGTTCAGCTTCTTCAGGTTCCTTGTATGACTATGCTTGATGTCAGTGGAAATTTCTTGTCAGGTTCAATGCCTGAATATGCTCACAGTACTTGTCGTCCTGTTTCCTCTGTGGATCTTTCTTTCAAAGATGATGATTTTTCCTCACCCTATGAAGCATTTTTTGGATCTAAAGCTCAAGCTGGAATGCCATTGCAATGGCCCACAGAGGATGATGTTGTTGTAGTGATTCACAACTTTGGGCACAATAATTTTACTGGCACTCTGCAGTCATTGCCCATTGCACCCGAAAGGTTTCGGAAGAAAATTCTGTATGCATTCCTTGTTGGAGAAAACAAGCTTACTGGAGCATTTCCAGGTAATTTGTTTGGGAAGTGTCAGGGTTTAGATTCACTGGTTGTTAATGTAAGCAACAACAGGTTAGATGGGAAGATTCCAACTGAAATTGGTAAAATGTGCGATTCTCTCAAGTTTTTAGATGCATCTCTGAATCAGATTGTGGGTTCCATTCCTGCTAGTTTTGGAGAATTGGTGTCTCTTGCTGGCCTTAACTTGAGTTCGAACAGGTTGCAAGGTCAAATTCCGACCACTCTTGGCCAGATAAGGGATCTGGAGTATCTTTCTTTGTCTGGTAATAACCTTACAGGTGTTATTCCTGCTAGTTTGGGGCAGCTTTACTCCTTGCATGTGCTTGAGCTTTCTCGGAACTCTCTCACTGGAGAGATACCAAAAGATCTTGTAAACTTGCGAAACTTGAGAGTTCTTCTGCTGGACAATAATAAACTTTCTGGCCAGATTCCATCTGGTTTGGCAAATGTGACCACGCTCTCAGCCTTCAATGTGTCTTACAATAACTTCTCTGGTTCATTGCCTTTGAACAATAACCTGGTGAATTGCAATACTGCTATTGGAAACCCCCATTTAAGTTCCTGTCCCTCACTTTCTCAAGTACAGCCAGCTAATACTCAAGGAAGAGTTGGCGATTCAGAACCTTATGCTGCTTCACCATTGGTAGGAACTCCGAAGAGTGCTGGTAGCGGTTTCAATTCAATTGAAATAGCATCCATTACATCTGCATCTGCCATTGTTTTAGTGCTTCTAGCTCTTGTTGTTCTATTCTTGTATACGCGGAAGTGGAATCGGAAGTCTGGAGGTCCAGGCTCTACTAGGAAGGAAGTTACCGTTTTCACAAACATTGGGGTTCCACTGACCTTTGATAGTGTTGTTCGGGGCACGGGGAGCTTTAATGCTAGCAACTGTATTGGGAATGGAGGTTTTGGAGCAACTTACAAGGCAGAGATATCACCTGGAGTCCTAGTGGCTATAAAACGGCTTGCTGTTGGAAGGTTCCAAGGAGTGCAACAGTTTCATGCAGAAATCAAGACTCTGGGGAGGCTTCGACATCCAAATCTGGTCACTTTACTAGGTTACCATGCCAGTGAAACAGAGATGTTCCTAATATATAATTATTTTCCTGGTGGTAATCTGGAAAAATTTATTCAGGAAAGGTCTACCAGGGCTGTGGATTGGAAGATACTTCACAAGATTGCTTTGGACATAGCCCGTGCACTTGCGTACCTACATGACCAGTGTGTACCTCGTGTCCTTCACCGTGATGTCAAGCCTAGCAATATTTTGTTGGATGATGATTTTAATGCATATCTATCAGACTTTGGTTTGGCCAGACTTCTTGGGACTTCAGAAACCCATGCTACTACTGGTGTAGCTGGGACCTTTGGATATGTTGCCCCAGAATATGCAATGACGTGTCGTGTTTCAGATAAGTCTGATGTGTATAGTTATGGTGTAGTGCTTCTTGAGTTGCTCTCTGACAAGAAAGTGTTGGATCCTTCCTTTTCTTCATATGGAAATGGATTCAACATCGTTGCATGGGCATGTATGCTTCTTCGACAAGGCCGTGCAAAGGAATTCTTTTCTGCAGGGTTGTGGGATGCAGGCCCCCATGATGATTTGGTTGAAGTTCTACACTTGGCAGTGGTATGTACGGTTGATTCTCTCTCGACCAGGCCTACTATGAGGCAAGTTGTTCGACGGCTGAAGCAACTTCAACCCCCTCATGTTAGGTGCCTGACGAGGTTTGTACTGGTTAACATTAGTATATGTGTAGAAAAGAGGAGCCATTGTAATCTGTGCTTTTGCACCGAGCCCTCTCTGGGGACTGGCTTTAGGTTAAATTCTAGAACTATAGTTACCCCACTTGTACAGTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000578 GO:0001101 GO:0003002 GO:0003006 GO:0005575 GO:0005623 GO:0005886 GO:0006725 GO:0006950 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009555 GO:0009628 GO:0009698 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009808 GO:0009846 GO:0009856 GO:0009880 GO:0009888 GO:0009908 GO:0009942 GO:0009945 GO:0009956 GO:0009987 GO:0010035 GO:0010073 GO:0010152 GO:0010154 GO:0016020 GO:0016043 GO:0019748 GO:0021700 GO:0022414 GO:0022607 GO:0032501 GO:0032502 GO:0042221 GO:0043933 GO:0044085 GO:0044237 GO:0044464 GO:0044706 GO:0048229 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048507 GO:0048508 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051259 GO:0051260 GO:0051704 GO:0061458 GO:0065003 GO:0071704 GO:0071840 GO:0071944 GO:0090567 GO:0099402 GO:1901360 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1192

Amino Acids

128.87

Weight (kDa)

6.87

Isoelectric Point (pI)

36.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	46 - 85	5e-10	Leucine rich repeat N-terminal domain
LRR_14	PF23598	267 - 391	3.1e-06	Leucine-rich repeat region
LRR_14	PF23598	643 - 728	3.3e-08	Leucine-rich repeat region
LRR_8	PF13855	653 - 711	3.3e-09	Leucine rich repeat
Pkinase	PF00069	866 - 1065	1.2e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	867 - 1135	7.4e-40	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0010808)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02130 AT3G02130
fragaria_vesca	FvH4_6g46830
malus_domestica	MD09G1067100.v1.1 MD17G1060000.v1.1 MD17G1060100.v1.1
prunus_persica	Prupe.3G254600_v2.0.a1
pyrus_communis	pycom111g05640 pycom17g05970 pycom17g05980
rosa_chinensis	RchiOBHm_Chr2g0165821
rosa_laevigata	RLG00000021558
rosa_multiflora	Rmu_sc0003227.1_g000004
rosa_roxburghii	Rroxscaffold_2G00085480
rosa_rugosa	Rorug02G0518000
rosa_samantha	Rh2AG583700 Rh2BG595700 Rh2CG566600 Rh2DG605500
rosa_wichuraiana	Rw2G048670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	702
AccB1I	GGYRCC	3 cut(s)	290, 296, 3432
AccBSI	CCGCTC	1 cut(s)	969
AccI	GTMKAC	2 cut(s)	2471, 2858
AccII	CGCG	3 cut(s)	104, 257, 2476
AccIII	TCCGGA	1 cut(s)	239
AclWI	GGATC	5 cut(s)	1470, 1522, 1962, 3188, 3201
AcoI	YGGCCR	4 cut(s)	919, 1942, 2137, 3027
AcuI	CTGAAG	6 cut(s)	143, 911, 1184, 1346, 3030, 3425
AcyI	GRCGYC	4 cut(s)	110, 123, 291, 297
AfaI	GTAC	8 cut(s)	728, 1438, 2298, 2924, 2943, 3352, 3450, 3570
AfiI	CCNNNNNNNGG	7 cut(s)	362, 473, 1951, 2360, 3271, 3521, 3522
AflIII	ACRYGT	1 cut(s)	3113
AjiI	CACGTC	1 cut(s)	3114
AjnI	CCWGG	8 cut(s)	233, 1702, 2232, 2503, 2653, 2821, 2861, 3371
AjuI	GAANNNNNNNTTGG	2 cut(s)	1494, 1526
Alw21I	GWGCWC	2 cut(s)	326, 2917
Alw26I	GTCTC	2 cut(s)	131, 1887
Alw44I	GTGCAC	1 cut(s)	2913
AlwI	GGATC	5 cut(s)	1470, 1522, 1962, 3188, 3201
AlwNI	CAGNNNCTG	1 cut(s)	228
Ama87I	CYCGRG	1 cut(s)	981
Aor13HI	TCCGGA	1 cut(s)	239
AoxI	GGCC	9 cut(s)	919, 1547, 1891, 1942, 2137, 3027, 3262, 3304, 3374
ApaLI	GTGCAC	1 cut(s)	2913
ApeKI	GCWGC	7 cut(s)	76, 154, 931, 1200, 1296, 2017, 2345
ArsI	GACNNNNNNTTYG	4 cut(s)	159, 191, 544, 576
Asp700I	GAANNNNTTC	2 cut(s)	903, 1641
AspLEI	GCGC	4 cut(s)	104, 293, 299, 1223
AspS9I	GGNCC	8 cut(s)	53, 237, 526, 1178, 1548, 2501, 3078, 3305
AsuC2I	CCSGG	4 cut(s)	51, 556, 680, 699
AsuII	TTCGAA	2 cut(s)	884, 1907
AsuNHI	GCTAGC	1 cut(s)	2597
AvaI	CYCGRG	1 cut(s)	981
AvaII	GGWCC	6 cut(s)	53, 237, 526, 1178, 2501, 3078
BaeGI	GKGCMC	3 cut(s)	1593, 2582, 2917
BaeI	ACNNNNGTAYC	2 cut(s)	2906, 2939
BalI	TGGCCA	3 cut(s)	1944, 2139, 3029
BamHI	GGATCC	1 cut(s)	3193
BanI	GGYRCC	3 cut(s)	290, 296, 3432
BanII	GRGCYC	3 cut(s)	212, 326, 3516
BauI	CACGAG	1 cut(s)	2946
Bbv12I	GWGCWC	2 cut(s)	326, 2917
BbvCI	CCTCAGC	1 cut(s)	863
BbvI	GCAGC	7 cut(s)	88, 141, 918, 1212, 1283, 2029, 2332
BccI	CCATC	4 cut(s)	476, 770, 1766, 2155
BceAI	ACGGC	6 cut(s)	136, 550, 703, 2693, 3249, 3416
BcgI	CGANNNNNNTGC	4 cut(s)	1897, 1931, 3377, 3411
BciT130I	CCWGG	8 cut(s)	235, 1704, 2234, 2505, 2655, 2823, 2863, 3373
BclI	TGATCA	1 cut(s)	1242
BcnI	CCSGG	4 cut(s)	51, 556, 680, 699
BcoDI	GTCTC	2 cut(s)	131, 1887
BfmI	CTRYAG	4 cut(s)	74, 1613, 3285, 3552
BfoI	RGCGCY	3 cut(s)	294, 300, 1224
BfuAI	ACCTGC	1 cut(s)	702
BglII	AGATCT	3 cut(s)	657, 1167, 2080
BmcAI	AGTACT	1 cut(s)	1438
Bme18I	GGWCC	6 cut(s)	53, 237, 526, 1178, 2501, 3078
BmeT110I	CYCGRG	1 cut(s)	981
BmgBI	CACGTC	1 cut(s)	3114
BmgT120I	GGNCC	8 cut(s)	53, 237, 526, 1178, 1548, 2501, 3078, 3305
BmrI	ACTGGG	2 cut(s)	1278, 1350
BmsI	GCATC	7 cut(s)	155, 805, 1816, 1838, 2417, 2432, 3289
BmtI	GCTAGC	1 cut(s)	2601
BmuI	ACTGGG	2 cut(s)	1278, 1350
BplI	GAGNNNNNCTC	2 cut(s)	3158, 3190
BpmI	CTGGAG	5 cut(s)	1713, 1979, 2085, 2517, 2676
Bpu10I	CCTNAGC	3 cut(s)	863, 965, 1322
Bpu14I	TTCGAA	2 cut(s)	884, 1907
BpuMI	CCSGG	4 cut(s)	51, 556, 680, 699
BsaBI	GATNNNNATC	1 cut(s)	632
BsaHI	GRCGYC	4 cut(s)	110, 123, 291, 297
BsaJI	CCNNGG	2 cut(s)	2698, 2862
BsaWI	WCCGGW	2 cut(s)	25, 239
BsaXI	ACNNNNNCTCC	8 cut(s)	370, 400, 1863, 1893, 2649, 2679, 3475, 3505
Bsc4I	CCNNNNNNNGG	7 cut(s)	362, 473, 1951, 2360, 3271, 3521, 3522
Bse118I	RCCGGY	4 cut(s)	287, 293, 299, 921
Bse3DI	GCAATG	6 cut(s)	1538, 1550, 1619, 1626, 2213, 3114
Bse8I	GATNNNNATC	1 cut(s)	632
BseAI	TCCGGA	1 cut(s)	239
BseBI	CCWGG	8 cut(s)	235, 1704, 2234, 2505, 2655, 2823, 2863, 3373
BseDI	CCNNGG	2 cut(s)	2698, 2862
BseGI	GGATG	6 cut(s)	952, 1564, 2408, 2749, 2995, 3304
BseJI	GATNNNNATC	1 cut(s)	632
BseLI	CCNNNNNNNGG	7 cut(s)	362, 473, 1951, 2360, 3271, 3521, 3522
BseMI	GCAATG	6 cut(s)	1538, 1550, 1619, 1626, 2213, 3114
BseMII	CTCAG	8 cut(s)	186, 435, 760, 854, 956, 1016, 1268, 2190
BseRI	GAGGAG	2 cut(s)	394, 3496
BseSI	GKGCMC	3 cut(s)	1593, 2582, 2917
BseX3I	CGGCCG	1 cut(s)	919
BseXI	GCAGC	7 cut(s)	88, 141, 918, 1212, 1283, 2029, 2332
BseYI	CCCAGC	4 cut(s)	204, 671, 988, 3074
BsgI	GTGCAG	1 cut(s)	214
Bsh1236I	CGCG	3 cut(s)	104, 257, 2476
Bsh1285I	CGRYCG	1 cut(s)	922
BshFI	GGCC	9 cut(s)	921, 1549, 1893, 1944, 2139, 3029, 3264, 3306, 3376
BshNI	GGYRCC	3 cut(s)	290, 296, 3432
BsiEI	CGRYCG	1 cut(s)	922
BsiHKAI	GWGCWC	2 cut(s)	326, 2917
BsiHKCI	CYCGRG	1 cut(s)	981
BslFI	GGGAC	8 cut(s)	66, 97, 1006, 1191, 2265, 3055, 3091, 3538
BslI	CCNNNNNNNGG	7 cut(s)	362, 473, 1951, 2360, 3271, 3521, 3522
BsmAI	GTCTC	2 cut(s)	131, 1887
BsmBI	CGTCTC	1 cut(s)	131
BsmFI	GGGAC	8 cut(s)	66, 97, 1006, 1191, 2265, 3055, 3091, 3538
BsmI	GAATGC	3 cut(s)	903, 1539, 1664
BsnI	GGCC	9 cut(s)	921, 1549, 1893, 1944, 2139, 3029, 3264, 3306, 3376
BsoBI	CYCGRG	1 cut(s)	981
Bsp119I	TTCGAA	2 cut(s)	884, 1907
Bsp1286I	GDGCHC	6 cut(s)	212, 326, 1593, 2582, 2917, 3516
Bsp13I	TCCGGA	1 cut(s)	239
Bsp1407I	TGTACA	1 cut(s)	3568
BspANI	GGCC	9 cut(s)	921, 1549, 1893, 1944, 2139, 3029, 3264, 3306, 3376
BspCNI	CTCAG	8 cut(s)	185, 436, 759, 855, 957, 1015, 1267, 2189
BspEI	TCCGGA	1 cut(s)	239
BspFNI	CGCG	3 cut(s)	104, 257, 2476
BspMAI	CTGCAG	3 cut(s)	78, 1617, 3289
BspMI	ACCTGC	1 cut(s)	702
BspOI	GCTAGC	1 cut(s)	2601
BspPI	GGATC	5 cut(s)	1470, 1522, 1962, 3188, 3201
BspT104I	TTCGAA	2 cut(s)	884, 1907
BspT107I	GGYRCC	3 cut(s)	290, 296, 3432
BsrBI	CCGCTC	1 cut(s)	969
BsrDI	GCAATG	6 cut(s)	1538, 1550, 1619, 1626, 2213, 3114
BsrFI	RCCGGY	4 cut(s)	287, 293, 299, 921
BsrGI	TGTACA	1 cut(s)	3568
BssAI	RCCGGY	4 cut(s)	287, 293, 299, 921
BssECI	CCNNGG	2 cut(s)	2698, 2862
BssNAI	GTATAC	1 cut(s)	2472
BssNI	GRCGYC	4 cut(s)	110, 123, 291, 297
BssSI	CACGAG	1 cut(s)	2946
BssT1I	CCWWGG	1 cut(s)	2698
Bst1107I	GTATAC	1 cut(s)	2472
Bst2BI	CACGAG	1 cut(s)	2946
Bst2UI	CCWGG	8 cut(s)	235, 1704, 2234, 2505, 2655, 2823, 2863, 3373
Bst4CI	ACNGT	8 cut(s)	623, 1436, 2530, 2606, 2712, 2960, 3355, 3573
Bst6I	CTCTTC	2 cut(s)	2314, 2366
BstACI	GRCGYC	4 cut(s)	110, 123, 291, 297
BstAPI	GCANNNNNTGC	1 cut(s)	1621
BstAUI	TGTACA	1 cut(s)	3568
BstBI	TTCGAA	2 cut(s)	884, 1907
BstEII	GGTNACC	2 cut(s)	28, 2774
BstF5I	GGATG	6 cut(s)	952, 1564, 2408, 2749, 2995, 3304
BstFNI	CGCG	3 cut(s)	104, 257, 2476
BstH2I	RGCGCY	3 cut(s)	294, 300, 1224
BstHHI	GCGC	4 cut(s)	104, 293, 299, 1223
BstMAI	GTCTC	2 cut(s)	131, 1887
BstMCI	CGRYCG	1 cut(s)	922
BstNI	CCWGG	8 cut(s)	235, 1704, 2234, 2505, 2655, 2823, 2863, 3373
BstNSI	RCATGY	2 cut(s)	2036, 3246
BstPI	GGTNACC	2 cut(s)	28, 2774
BstSFI	CTRYAG	4 cut(s)	74, 1613, 3285, 3552
BstSLI	GKGCMC	3 cut(s)	1593, 2582, 2917
BstUI	CGCG	3 cut(s)	104, 257, 2476
BstV1I	GCAGC	7 cut(s)	88, 141, 918, 1212, 1283, 2029, 2332
BstX2I	RGATCY	7 cut(s)	657, 1167, 1462, 1514, 1954, 2080, 3193
BstXI	CCANNNNNNTGG	2 cut(s)	1545, 2869
BstYI	RGATCY	7 cut(s)	657, 1167, 1462, 1514, 1954, 2080, 3193
BstZ17I	GTATAC	1 cut(s)	2472
BstZI	CGGCCG	1 cut(s)	919
BsuRI	GGCC	9 cut(s)	921, 1549, 1893, 1944, 2139, 3029, 3264, 3306, 3376
BtrI	CACGTC	1 cut(s)	3114
BtsCI	GGATG	6 cut(s)	952, 1564, 2408, 2749, 2995, 3304
BtsI	GCAGTG	2 cut(s)	226, 3348
BveI	ACCTGC	1 cut(s)	702
CaiI	CAGNNNCTG	1 cut(s)	228
CfoI	GCGC	4 cut(s)	104, 293, 299, 1223
Cfr10I	RCCGGY	4 cut(s)	287, 293, 299, 921
Cfr13I	GGNCC	8 cut(s)	53, 237, 526, 1178, 1548, 2501, 3078, 3305
CseI	GACGC	5 cut(s)	99, 112, 152, 263, 877
CsiI	ACCWGGT	2 cut(s)	233, 2232
Csp6I	GTAC	8 cut(s)	727, 1437, 2297, 2923, 2942, 3351, 3449, 3569
CviQI	GTAC	8 cut(s)	727, 1437, 2297, 2923, 2942, 3351, 3449, 3569
DinI	GGCGCC	2 cut(s)	292, 298
EaeI	YGGCCR	4 cut(s)	919, 1942, 2137, 3027
EagI	CGGCCG	1 cut(s)	919
Eam1104I	CTCTTC	2 cut(s)	2314, 2366
EarI	CTCTTC	2 cut(s)	2314, 2366
EciI	GGCGGA	2 cut(s)	149, 318
Ecl136II	GAGCTC	1 cut(s)	324
EclXI	CGGCCG	1 cut(s)	919
Eco130I	CCWWGG	1 cut(s)	2698
Eco147I	AGGCCT	1 cut(s)	3376
Eco24I	GRGCYC	3 cut(s)	212, 326, 3516
Eco32I	GATATC	1 cut(s)	2649
Eco47I	GGWCC	6 cut(s)	53, 237, 526, 1178, 2501, 3078
Eco52I	CGGCCG	1 cut(s)	919
Eco53kI	GAGCTC	1 cut(s)	324
Eco57I	CTGAAG	6 cut(s)	143, 911, 1184, 1346, 3030, 3425
Eco88I	CYCGRG	1 cut(s)	981
Eco91I	GGTNACC	2 cut(s)	28, 2774
EcoICRI	GAGCTC	1 cut(s)	324
EcoO109I	RGGNCCY	4 cut(s)	53, 526, 3078, 3305
EcoO65I	GGTNACC	2 cut(s)	28, 2774
EcoRI	GAATTC	1 cut(s)	3275
EcoRII	CCWGG	8 cut(s)	233, 1702, 2232, 2503, 2653, 2821, 2861, 3371
EcoRV	GATATC	1 cut(s)	2649
EcoT14I	CCWWGG	1 cut(s)	2698
EcoT22I	ATGCAT	3 cut(s)	1666, 1831, 3007
EcoT38I	GRGCYC	3 cut(s)	212, 326, 3516
EgeI	GGCGCC	2 cut(s)	292, 298
EheI	GGCGCC	2 cut(s)	292, 298
ErhI	CCWWGG	1 cut(s)	2698
Esp3I	CGTCTC	1 cut(s)	131
FalI	AAGNNNNNCTT	4 cut(s)	180, 212, 3263, 3295
FaqI	GGGAC	8 cut(s)	66, 97, 1006, 1191, 2265, 3055, 3091, 3538
FauI	CCCGC	1 cut(s)	1277
FauNDI	CATATG	1 cut(s)	3211
FbaI	TGATCA	1 cut(s)	1242
FblI	GTMKAC	2 cut(s)	2471, 2858
FokI	GGATG	6 cut(s)	959, 1571, 2395, 2736, 3002, 3311
FriOI	GRGCYC	3 cut(s)	212, 326, 3516
GlaI	GCGC	4 cut(s)	103, 292, 298, 1222
GsaI	CCCAGC	4 cut(s)	208, 675, 992, 3078
GsuI	CTGGAG	5 cut(s)	1713, 1979, 2085, 2517, 2676
HaeII	RGCGCY	3 cut(s)	294, 300, 1224
HaeIII	GGCC	9 cut(s)	921, 1549, 1893, 1944, 2139, 3029, 3264, 3306, 3376
HgaI	GACGC	5 cut(s)	99, 112, 152, 263, 877
HhaI	GCGC	4 cut(s)	104, 293, 299, 1223
Hin1I	GRCGYC	4 cut(s)	110, 123, 291, 297
Hin6I	GCGC	4 cut(s)	102, 291, 297, 1221
HinP1I	GCGC	4 cut(s)	102, 291, 297, 1221
HincII	GTYRAC	1 cut(s)	3457
HindII	GTYRAC	1 cut(s)	3457
HindIII	AAGCTT	4 cut(s)	788, 1304, 1334, 1685
HpaI	GTTAAC	1 cut(s)	3457
Hpy166II	GTNNAC	9 cut(s)	577, 685, 727, 2089, 2472, 2859, 2915, 2942, 3457
Hpy8I	GTNNAC	9 cut(s)	577, 685, 727, 2089, 2472, 2859, 2915, 2942, 3457
Hpy99I	CGWCG	4 cut(s)	128, 257, 1030, 3402
HpyCH4III	ACNGT	8 cut(s)	623, 1436, 2530, 2606, 2712, 2960, 3355, 3573
HpyCH4IV	ACGT	1 cut(s)	3113
HpySE526I	ACGT	1 cut(s)	3113
Hsp92I	GRCGYC	4 cut(s)	110, 123, 291, 297
HspAI	GCGC	4 cut(s)	102, 291, 297, 1221
KasI	GGCGCC	2 cut(s)	290, 296
KflI	GGGWCCC	1 cut(s)	53
Kpn2I	TCCGGA	1 cut(s)	239
KroI	GCCGGC	3 cut(s)	293, 299, 921
KroNI	GCCGGC	3 cut(s)	295, 301, 923
Ksp22I	TGATCA	1 cut(s)	1242
KspAI	GTTAAC	1 cut(s)	3457
LmnI	GCTCC	9 cut(s)	329, 423, 695, 1084, 1345, 1694, 2586, 2627, 3483
Lsp1109I	GCAGC	7 cut(s)	88, 141, 918, 1212, 1283, 2029, 2332
LweI	GCATC	7 cut(s)	155, 805, 1816, 1838, 2417, 2432, 3289
MabI	ACCWGGT	2 cut(s)	233, 2232
MaeII	ACGT	1 cut(s)	3113
MaeIII	GTNAC	8 cut(s)	28, 224, 812, 2164, 2524, 2761, 2774, 3556
MbiI	CCGCTC	1 cut(s)	969
MfeI	CAATTG	2 cut(s)	739, 2397
MflI	RGATCY	7 cut(s)	657, 1167, 1462, 1514, 1954, 2080, 3193
MhlI	GDGCHC	6 cut(s)	212, 326, 1593, 2582, 2917, 3516
MlsI	TGGCCA	3 cut(s)	1944, 2139, 3029
MluNI	TGGCCA	3 cut(s)	1944, 2139, 3029
Mly113I	GGCGCC	2 cut(s)	291, 297
MlyI	GAGTC	7 cut(s)	164, 176, 252, 635, 1067, 2667, 2725
MmeI	TCCRAC	9 cut(s)	873, 924, 1062, 1654, 1808, 1955, 2668, 2967, 3171
Mox20I	TGGCCA	3 cut(s)	1944, 2139, 3029
Mph1103I	ATGCAT	3 cut(s)	1666, 1831, 3007
MreI	CGCCGGCG	2 cut(s)	293, 299
MroI	TCCGGA	1 cut(s)	239
MroNI	GCCGGC	3 cut(s)	293, 299, 921
MroXI	GAANNNNTTC	2 cut(s)	903, 1641
MscI	TGGCCA	3 cut(s)	1944, 2139, 3029
MslI	CAYNNNNRTG	4 cut(s)	1543, 2784, 2961, 3241
Msp20I	TGGCCA	3 cut(s)	1944, 2139, 3029
MspA1I	CMGCKG	3 cut(s)	67, 931, 1296
MunI	CAATTG	2 cut(s)	739, 2397
Mva1269I	GAATGC	3 cut(s)	903, 1539, 1664
MvaI	CCWGG	8 cut(s)	235, 1704, 2234, 2505, 2655, 2823, 2863, 3373
MvnI	CGCG	3 cut(s)	104, 257, 2476
NaeI	GCCGGC	3 cut(s)	295, 301, 923
NarI	GGCGCC	2 cut(s)	291, 297
NciI	CCSGG	4 cut(s)	51, 556, 680, 699
NdeI	CATATG	1 cut(s)	3211
NgoMIV	GCCGGC	3 cut(s)	293, 299, 921
NheI	GCTAGC	1 cut(s)	2597
NmeAIII	GCCGAG	1 cut(s)	897
NmuCI	GTSAC	4 cut(s)	28, 224, 2164, 2761
NsiI	ATGCAT	3 cut(s)	1666, 1831, 3007
NspI	RCATGY	2 cut(s)	2036, 3246
NspV	TTCGAA	2 cut(s)	884, 1907
PaeR7I	CTCGAG	1 cut(s)	981
PceI	AGGCCT	1 cut(s)	3376
PcsI	WCGNNNNNNNCGW	1 cut(s)	3262
PctI	GAATGC	3 cut(s)	903, 1539, 1664
PdiI	GCCGGC	3 cut(s)	295, 301, 923
PdmI	GAANNNNTTC	2 cut(s)	903, 1641
PflFI	GACNNNGTC	3 cut(s)	173, 556, 644
PfoI	TCCNGGA	1 cut(s)	49
PleI	GAGTC	7 cut(s)	164, 176, 251, 635, 1067, 2666, 2725
PluTI	GGCGCC	2 cut(s)	294, 300
PpsI	GAGTC	7 cut(s)	164, 176, 251, 635, 1067, 2666, 2725
PpuMI	RGGWCCY	3 cut(s)	53, 526, 3078
Psp124BI	GAGCTC	1 cut(s)	326
Psp5II	RGGWCCY	3 cut(s)	53, 526, 3078
Psp6I	CCWGG	8 cut(s)	233, 1702, 2232, 2503, 2653, 2821, 2861, 3371
PspEI	GGTNACC	2 cut(s)	28, 2774
PspFI	CCCAGC	4 cut(s)	204, 671, 988, 3074
PspGI	CCWGG	8 cut(s)	233, 1702, 2232, 2503, 2653, 2821, 2861, 3371
PspPI	GGNCC	8 cut(s)	53, 237, 526, 1178, 1548, 2501, 3078, 3305
PspPPI	RGGWCCY	3 cut(s)	53, 526, 3078
PsrI	GAACNNNNNNTAC	2 cut(s)	3541, 3573
PstI	CTGCAG	3 cut(s)	78, 1617, 3289
PstNI	CAGNNNCTG	1 cut(s)	228
PsuI	RGATCY	7 cut(s)	657, 1167, 1462, 1514, 1954, 2080, 3193
PsyI	GACNNNGTC	3 cut(s)	173, 556, 644
PvuII	CAGCTG	1 cut(s)	931
RsaI	GTAC	8 cut(s)	728, 1438, 2298, 2924, 2943, 3352, 3450, 3570
RsaNI	GTAC	8 cut(s)	727, 1437, 2297, 2923, 2942, 3351, 3449, 3569
RseI	CAYNNNNRTG	4 cut(s)	1543, 2784, 2961, 3241
SacI	GAGCTC	1 cut(s)	326
Sau96I	GGNCC	8 cut(s)	53, 237, 526, 1178, 1548, 2501, 3078, 3305
ScaI	AGTACT	1 cut(s)	1438
SchI	GAGTC	7 cut(s)	164, 176, 252, 635, 1067, 2667, 2725
SduI	GDGCHC	6 cut(s)	212, 326, 1593, 2582, 2917, 3516
SexAI	ACCWGGT	2 cut(s)	233, 2232
SfaNI	GCATC	7 cut(s)	155, 805, 1816, 1838, 2417, 2432, 3289
SfcI	CTRYAG	4 cut(s)	74, 1613, 3285, 3552
SfoI	GGCGCC	2 cut(s)	292, 298
Sfr274I	CTCGAG	1 cut(s)	981
SfuI	TTCGAA	2 cut(s)	884, 1907
SgrAI	CRCCGGYG	2 cut(s)	293, 299
SinI	GGWCC	6 cut(s)	53, 237, 526, 1178, 2501, 3078
SlaI	CTCGAG	1 cut(s)	981
SmiMI	CAYNNNNRTG	4 cut(s)	1543, 2784, 2961, 3241
SseBI	AGGCCT	1 cut(s)	3376
SspDI	GGCGCC	2 cut(s)	290, 296
SspI	AATATT	1 cut(s)	2980
SstI	GAGCTC	1 cut(s)	326
StuI	AGGCCT	1 cut(s)	3376
StyI	CCWWGG	1 cut(s)	2698
TaaI	ACNGT	8 cut(s)	623, 1436, 2530, 2606, 2712, 2960, 3355, 3573
TaiI	ACGT	1 cut(s)	3116
TaqII	GACCGA	2 cut(s)	202, 1195
TatI	WGTACW	5 cut(s)	726, 1436, 2296, 3448, 3568
TauI	GCSGC	3 cut(s)	67, 107, 110
TseFI	GTSAC	4 cut(s)	28, 224, 2164, 2761
TseI	GCWGC	7 cut(s)	76, 154, 931, 1200, 1296, 2017, 2345
Tsp45I	GTSAC	4 cut(s)	28, 224, 2164, 2761
TspDTI	ATGAA	7 cut(s)	815, 1115, 1298, 1515, 2202, 2705, 3198
TspGWI	ACGGA	5 cut(s)	155, 254, 321, 345, 682
Tth111I	GACNNNGTC	3 cut(s)	173, 556, 644
VneI	GTGCAC	1 cut(s)	2913
VpaK11BI	GGWCC	6 cut(s)	53, 237, 526, 1178, 2501, 3078
XbaI	TCTAGA	1 cut(s)	3545
XceI	RCATGY	2 cut(s)	2036, 3246
XcmI	CCANNNNNNNNNTGG	3 cut(s)	64, 2147, 3062
XhoI	CTCGAG	1 cut(s)	981
XmiI	GTMKAC	2 cut(s)	2471, 2858
XmnI	GAANNNNTTC	2 cut(s)	903, 1641
ZrmI	AGTACT	1 cut(s)	1438
Zsp2I	ATGCAT	3 cut(s)	1666, 1831, 3007

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.