Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0010808

Physical Location & Seq

Genomic Coordinates Forward (+)

4868626 .. 4873023

Gene Structure 4398 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1060000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3168 bp

ATGGGTTTTTCTCTATCTTCCTCTTCGGTCATCAAAGGACATGTTTTTCGCAGACCCACTTCTCCAGTGTTTGTTCTGAAGCTTGTTTTGCTCTTGTGGGTGTTCTTTACTTCTCAGAACTGTGCTGTTCTCGCTGATACTGACGCATCGGTGCTTCTTGAGCTAAAAATCTCTGTCTTGGATCCGTCGGGCTTGCTTTCTGGCTGGGATGGTCGGCTCAATTCTGGTCATTGCTCATGGCCTGGCGTCTTCTGCAACTCAAATTCTCGGGTGGTTTCACTCAACATTACTGGGAATGGAGGAGAAACCGAAGGTAGATCGAAAACTATTGCATGCTCAGATTTTGCTCAGTTGCCGCTTTATGGATTTGGAATCAGGAGGAGCTGTGAGGGTAGTAGAGGAAGATTGGCTGGGAAGCTCCCGCCTGTAATTGGTAAGCTCACTGAGCTTAGGGTTTTGTCCCTCCCCTTCAATGGTTTTGAGGGTGAAATTCCTAGTGAAGTTTTGGGCTTGGAAAATTTGGAGGTTCTTGATCTTGAGGGAAACTCGATAACTGGGTCGCTGCCCTTTCCGCTTAATCCAAATTTGAGGGTTCTTAATCTGGGGTTTAATAGGATTGAAGGTGAGATACCGACCTCGTGGTCAAATTATATGAGCCTAGAGATCTTGAATTTAGCTGGGAATCTCGTAAACGGGACAATTCCAGGTTTCATTGGTAGGTTAAAGGCGGTGTACTTGTCATACAATTCACTTAGTGGAGATGTTCCCAGCGAGATTGGAGCCAAGTGTGGGAAGCTTGAGCATCTTGATTTGGCGGGTAATTTCTTGGTTCATAAAATTCCAAGCAGTTTGGGAAATTGCAGCCAGTTGAGGACGTTGTTGCTGTATTCAAATATGTTTGAAGAGGGTATTCCAGCTGAACTTGGACGGCTTCAAGCTCTGGAAGTGTTGGATGTGTCGAGGAATAGCCTGAGTAGTTCATTACCACCCGAGCTGGGGAATTGTTCTCAGTTGTCTGCCCTTGTGCTGTCATCTATGTTTAATCCACTTCCACTAGTCAATGATACTGTAGTGGACTCTTTGCTGGAGCAGTTGAATGCTATGAGTGACGACTTCAATTACTTTCAAGGTACAATGCCTGTAGAAATTACAACCCTTCCAAAGCTAAGGATCTTGTGGGCACCAAGGGCAAGTATTGAGGGCACTTTCCCAAGCAATTGGGGTGGTTGTGAGTACTTAGAGATGATCAATTTGGCTCAGAACTTTTTGACTGGGGAAATTCCTAGCGGACTTAGTCGCTGCAGGAACCTTCAATTTCTTGACGTGAGCTCTAACAGGCTTAGTGGGGAGCTTTTTCAGGATCTTCATGTTCCTTGTATGGATATGTTTGATATCAGCGGAAACATCTTGTCAGGTTCAATACCTGAATATTTCAACAAAACTTGTGGTTCTCCTTTGAGAGATTTTTCTTTCAAGTATGAGGATCCGTCCTCACACTATGTAGCATTTTTTGCTTCTAAATCCCAAGTTGGAAACCCATTGCAAATGCATGGGGAGGTTGATGGTCTTATAGTAGCACACAACTTTGCGCACAATAATTTTACTGGCACTTTGCAGACACTGCCTATTGCACCTGAAAGACTTGGGAAGCAAACTTTTTATGCATTCTTTGCTGGAGAAAACAAGCTTTCTGGAGAATTTCCAGGTAACTTATTTGGCAAGTGTGAGGGATTGGAGTGGCTGATTGTTAATGTTAGCCACAACAAGTTAGAAGGTCAGATTCCTGCTGAATTTGGCACTATGTGCAAATCTCTTAAAGTTTTAGATGCATCTCGGAATCAGATGGTTGGTCCTATTCCCCCTACCATTGGGAAATTGTCTCTTGTCGCCCTTAATTTGAGTTGGAACATGTTGAAGGGTGAAATACCAACCAGTCTTGGCCAGATAACTGATCTGACGTATCTATCCTTATCTGGCAATCTCCTTACAGGTGTCATTCCGTCTAGCTTGGGGCAGCTTTCCTCTTTAGAAGTGCTTGAACTTTCTTCAAACTATCTCACTGGCGAGATACCAAATGATCTTGTGAATCTGCGAAACCTGACTGCTCTTCTGCTGGACAAGAATAATATTTCTGGTCAGATTCCATCTGGATTGGCAAATATGACCACACTGTCATCTTTCAATGTGTCTTTCAATAACATATCAGGTTCACTGCCTGCGAATAATAACCTGATGAAATGCAGTGCTGCTATTGGAAACCCAAATCTACACTCTTGCCCCATGTTGTCTCAGACACAGCCAGCTTTGGATTCCCAAGGAAGAGTTGGCGATTCACAACCTCTTGCTGCTTCACCAATGGGAGGTCCAGCTCAGAGGAGTGGGAATGGTTTCAGTCCAATTGAGATAGCATCCATTACATCTGCATCGGCCATAGTTTCAGTGCTGCTTGCTCTTGTTGTTCTGTTCTTGTATACACGGAAATGGAATCCAAAGTCTGGAACTCAAGTGTCTACCCGGAAGGAAGTTACAGTTTTCACAAACATTGGGGTTCCGTTGACCTTTGAGAATGTTGTGCGAGGCACAGGAAGTTTCAATGCTAGCAACTGCATCGGGAATGGAGGTTTTGGAGCTACCTACAAGGCAGAGATATCACCTGGAATCGTGGTGGCAATTAAACGGCTTTCAGTTGGAAGGTTCCAAGGAGTTCAACAGTTTCATGCAGAAATTAAAACCCTTGGTAGGCTTCGGCATCCAAATCTTGTCACTTTGCTTGGTTATCACGCCAAGACTCATGCTACTACCGGTGTTGCTGGAACGTTTGGATATGTTGCCCCGGAATATGCAATGACTTGCCGAGTTTCAGATAAGGCTGATGTATATAGTTATGGCGTTGTGCTACTGGAGTTACTCTCTGACAAGAAGGCACTGGATCCCTCCTTCTCTTCGTATGGAAATGGATTCAACATCGTTCAGTGGTCATGTATGCTTCTTCGGCAAGGCCGGGCAAAGGAATTCTTCACCGCTGGGTTATGGGATGCAGGTCCCCATGATGACTTAGTAGAAGTTCTTCACTTGGCAGTAGTGTGCACGGTTGACTCTCTCTCAACTAGGCCTACAATGAGGCAAGTTGTACGACGGCTTAAGCAACTTCAGCCTCCATCTTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000578 GO:0001101 GO:0003002 GO:0003006 GO:0005575 GO:0005623 GO:0005886 GO:0006725 GO:0006950 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009555 GO:0009628 GO:0009698 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009808 GO:0009846 GO:0009856 GO:0009880 GO:0009888 GO:0009908 GO:0009942 GO:0009945 GO:0009956 GO:0009987 GO:0010035 GO:0010073 GO:0010152 GO:0010154 GO:0016020 GO:0016043 GO:0019748 GO:0021700 GO:0022414 GO:0022607 GO:0032501 GO:0032502 GO:0042221 GO:0043933 GO:0044085 GO:0044237 GO:0044464 GO:0044706 GO:0048229 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048507 GO:0048508 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051259 GO:0051260 GO:0051704 GO:0061458 GO:0065003 GO:0071704 GO:0071840 GO:0071944 GO:0090567 GO:0099402 GO:1901360 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1056

Amino Acids

113.89

Weight (kDa)

6.15

Isoelectric Point (pI)

39.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	46 - 86	7.4e-09	Leucine rich repeat N-terminal domain
LRR_14	PF23598	213 - 367	7.6e-08	Leucine-rich repeat region
LRR_14	PF23598	645 - 727	3.9e-09	Leucine-rich repeat region
LRR_8	PF13855	652 - 710	2.3e-09	Leucine rich repeat
Pkinase	PF00069	932 - 977	5.3e-06	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	937 - 1047	9.6e-06	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0010808)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02130 AT3G02130
fragaria_vesca	FvH4_6g46830
malus_domestica	MD09G1067100.v1.1 MD17G1060000.v1.1 MD17G1060100.v1.1
prunus_persica	Prupe.3G254600_v2.0.a1
pyrus_communis	pycom111g05640 pycom17g05970 pycom17g05980
rosa_chinensis	RchiOBHm_Chr2g0165821
rosa_laevigata	RLG00000021558
rosa_multiflora	Rmu_sc0003227.1_g000004
rosa_roxburghii	Rroxscaffold_2G00085480
rosa_rugosa	Rorug02G0518000
rosa_samantha	Rh2AG583700 Rh2BG595700 Rh2CG566600 Rh2DG605500
rosa_wichuraiana	Rw2G048670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	640, 2170
Acc16I	TGCGCA	1 cut(s)	1590
Acc36I	ACCTGC	1 cut(s)	3029
AccB1I	GGYRCC	1 cut(s)	1180
AccB7I	CCANNNNNTGG	1 cut(s)	2495
AccI	GTMKAC	2 cut(s)	2471, 2510
AciI	CCGC	8 cut(s)	356, 422, 572, 728, 815, 1287, 1398, 3021
AclI	AACGTT	1 cut(s)	2816
AclWI	GGATC	8 cut(s)	176, 189, 1178, 1368, 1478, 1491, 2924, 2937
AcoI	YGGCCR	2 cut(s)	1939, 2427
AcuI	CTGAAG	2 cut(s)	98, 3134
AcyI	GRCGYC	1 cut(s)	246
AdeI	CACNNNGTG	2 cut(s)	755, 1803
AfaI	GTAC	4 cut(s)	734, 1132, 1235, 3132
AflII	CTTAAG	1 cut(s)	3140
AflIII	ACRYGT	2 cut(s)	40, 1908
AgeI	ACCGGT	1 cut(s)	2801
AhlI	ACTAGT	1 cut(s)	1054
AjiI	CACGTC	1 cut(s)	1324
AjnI	CCWGG	4 cut(s)	241, 703, 1702, 2653
Alw21I	GWGCWC	2 cut(s)	1331, 3089
Alw26I	GTCTC	2 cut(s)	1884, 2292
Alw44I	GTGCAC	1 cut(s)	3085
AlwI	GGATC	8 cut(s)	176, 189, 1178, 1368, 1478, 1491, 2924, 2937
AlwNI	CAGNNNCTG	1 cut(s)	1621
Ama87I	CYCGRG	2 cut(s)	267, 989
AoxI	GGCC	5 cut(s)	239, 1939, 2427, 2998, 3110
ApaLI	GTGCAC	1 cut(s)	3085
ApeKI	GCWGC	7 cut(s)	562, 861, 1299, 2014, 2246, 2345, 2443
ArsI	GACNNNNNNTTYG	2 cut(s)	30, 62
AsiGI	ACCGGT	1 cut(s)	2801
Asp700I	GAANNNNTTC	2 cut(s)	2387, 3066
AspLEI	GCGC	1 cut(s)	1591
AspS9I	GGNCC	3 cut(s)	1850, 2363, 3041
AsuC2I	CCSGG	3 cut(s)	2515, 2834, 3002
AsuHPI	GGTGA	6 cut(s)	497, 635, 1931, 2343, 2643, 3010
AsuNHI	GCTAGC	1 cut(s)	2597
AvaI	CYCGRG	2 cut(s)	267, 989
AvaII	GGWCC	3 cut(s)	1850, 2363, 3041
BaeGI	GKGCMC	3 cut(s)	1183, 1205, 3089
BaeI	ACNNNNGTAYC	2 cut(s)	1056, 1089
BalI	TGGCCA	1 cut(s)	1941
BamHI	GGATCC	3 cut(s)	181, 1483, 2929
BanI	GGYRCC	1 cut(s)	1180
BanII	GRGCYC	1 cut(s)	1331
BarI	GAAGNNNNNNTAC	4 cut(s)	612, 644, 3051, 3083
BauI	CACGAG	1 cut(s)	637
BbsI	GAAGAC	1 cut(s)	241
Bbv12I	GWGCWC	2 cut(s)	1331, 3089
BbvI	GCAGC	7 cut(s)	549, 873, 1286, 2026, 2233, 2332, 2430
BccI	CCATC	5 cut(s)	203, 1556, 1837, 2152, 3166
BceAI	ACGGC	3 cut(s)	944, 2693, 3152
BcgI	CGANNNNNNTGC	2 cut(s)	2590, 2624
BciT130I	CCWGG	4 cut(s)	243, 705, 1704, 2655
BclI	TGATCA	1 cut(s)	1245
BcnI	CCSGG	3 cut(s)	2515, 2834, 3002
BcoDI	GTCTC	2 cut(s)	1884, 2292
BcuI	ACTAGT	1 cut(s)	1054
BfaI	CTAG	8 cut(s)	495, 659, 1055, 1284, 2004, 2598, 3108, 3166
BfmI	CTRYAG	3 cut(s)	1068, 1140, 1300
BfrI	CTTAAG	1 cut(s)	3140
BfuAI	ACCTGC	1 cut(s)	3029
BglII	AGATCT	1 cut(s)	663
BisI	GCNGC	8 cut(s)	356, 563, 862, 1300, 2015, 2247, 2346, 2444
BlsI	GCNGC	8 cut(s)	357, 564, 863, 1301, 2016, 2248, 2347, 2445
BmcAI	AGTACT	1 cut(s)	1235
Bme1390I	CCNGG	7 cut(s)	243, 705, 1704, 2515, 2655, 2834, 3002
Bme18I	GGWCC	3 cut(s)	1850, 2363, 3041
BmeT110I	CYCGRG	2 cut(s)	267, 989
BmgBI	CACGTC	1 cut(s)	1324
BmgT120I	GGNCC	3 cut(s)	1850, 2363, 3041
BmiI	GGNNCC	9 cut(s)	183, 781, 1182, 1307, 1485, 2550, 2696, 2931, 3043
BmrFI	CCNGG	7 cut(s)	243, 705, 1704, 2515, 2655, 2834, 3002
BmrI	ACTGGG	3 cut(s)	300, 564, 1281
BmsI	GCATC	9 cut(s)	155, 811, 1816, 1838, 2417, 2432, 2616, 2758, 3025
BmtI	GCTAGC	1 cut(s)	2601
BmuI	ACTGGG	3 cut(s)	300, 564, 1281
BpiI	GAAGAC	1 cut(s)	241
BplI	GAGNNNNNCTC	4 cut(s)	530, 562, 2894, 2926
BpmI	CTGGAG	5 cut(s)	48, 1106, 1695, 1713, 2921
Bpu10I	CCTNAGC	2 cut(s)	449, 1166
BpuEI	CTTGAG	4 cut(s)	179, 557, 818, 2487
BpuMI	CCSGG	3 cut(s)	2515, 2834, 3002
BsaHI	GRCGYC	1 cut(s)	246
BsaJI	CCNNGG	5 cut(s)	1184, 2314, 2698, 2734, 2832
BsaWI	WCCGGW	1 cut(s)	2801
BsaXI	ACNNNNNCTCC	2 cut(s)	370, 400
Bse118I	RCCGGY	1 cut(s)	2801
Bse3DI	GCAATG	3 cut(s)	229, 1538, 2850
BseBI	CCWGG	4 cut(s)	243, 705, 1704, 2655
BseDI	CCNNGG	5 cut(s)	1184, 2314, 2698, 2734, 2832
BseGI	GGATG	5 cut(s)	214, 958, 2408, 2749, 3040
BseMI	GCAATG	3 cut(s)	229, 1538, 2850
BseMII	CTCAG	9 cut(s)	128, 351, 362, 435, 962, 1022, 1271, 2303, 2384
BseRI	GAGGAG	3 cut(s)	315, 394, 2389
BseSI	GKGCMC	3 cut(s)	1183, 1205, 3089
BseXI	GCAGC	7 cut(s)	549, 873, 1286, 2026, 2233, 2332, 2430
BseYI	CCCAGC	6 cut(s)	204, 410, 677, 767, 994, 3023
BshFI	GGCC	5 cut(s)	241, 1941, 2429, 3000, 3112
BshNI	GGYRCC	1 cut(s)	1180
BshTI	ACCGGT	1 cut(s)	2801
BsiHKAI	GWGCWC	2 cut(s)	1331, 3089
BsiHKCI	CYCGRG	2 cut(s)	267, 989
BsiSI	CCGG	4 cut(s)	2515, 2802, 2834, 3001
BslFI	GGGAC	3 cut(s)	445, 709, 3027
BsmAI	GTCTC	2 cut(s)	1884, 2292
BsmFI	GGGAC	3 cut(s)	445, 709, 3027
BsmI	GAATGC	2 cut(s)	1102, 1664
BsnI	GGCC	5 cut(s)	241, 1941, 2429, 3000, 3112
BsoBI	CYCGRG	2 cut(s)	267, 989
Bsp1286I	GDGCHC	4 cut(s)	1183, 1205, 1331, 3089
BspACI	CCGC	8 cut(s)	356, 422, 572, 728, 815, 1287, 1398, 3021
BspANI	GGCC	5 cut(s)	241, 1941, 2429, 3000, 3112
BspCNI	CTCAG	9 cut(s)	127, 350, 361, 436, 963, 1021, 1270, 2302, 2383
BspLI	GGNNCC	9 cut(s)	183, 781, 1182, 1307, 1485, 2550, 2696, 2931, 3043
BspMAI	CTGCAG	1 cut(s)	1304
BspMI	ACCTGC	1 cut(s)	3029
BspOI	GCTAGC	1 cut(s)	2601
BspPI	GGATC	8 cut(s)	176, 189, 1178, 1368, 1478, 1491, 2924, 2937
BspQI	GCTCTTC	1 cut(s)	2112
BspT107I	GGYRCC	1 cut(s)	1180
BspTI	CTTAAG	1 cut(s)	3140
BsrDI	GCAATG	3 cut(s)	229, 1538, 2850
BsrFI	RCCGGY	1 cut(s)	2801
BssAI	RCCGGY	1 cut(s)	2801
BssECI	CCNNGG	5 cut(s)	1184, 2314, 2698, 2734, 2832
BssNAI	GTATAC	1 cut(s)	2472
BssNI	GRCGYC	1 cut(s)	246
BssSI	CACGAG	1 cut(s)	637
BssT1I	CCWWGG	4 cut(s)	1184, 2314, 2698, 2734
Bst1107I	GTATAC	1 cut(s)	2472
Bst2BI	CACGAG	1 cut(s)	637
Bst2UI	CCWGG	4 cut(s)	243, 705, 1704, 2655
Bst4CI	ACNGT	6 cut(s)	122, 1069, 2172, 2530, 2712, 3091
Bst6I	CTCTTC	5 cut(s)	28, 897, 2112, 2314, 2947
BstACI	GRCGYC	1 cut(s)	246
BstAFI	CTTAAG	1 cut(s)	3140
BstAPI	GCANNNNNTGC	4 cut(s)	1511, 1621, 1670, 1803
BstC8I	GCNNGC	6 cut(s)	194, 334, 2217, 2301, 2448, 2599
BstF5I	GGATG	5 cut(s)	214, 958, 2408, 2749, 3040
BstHHI	GCGC	1 cut(s)	1591
BstMAI	GTCTC	2 cut(s)	1884, 2292
BstNI	CCWGG	4 cut(s)	243, 705, 1704, 2655
BstNSI	RCATGY	3 cut(s)	44, 336, 1912
BstSCI	CCNGG	7 cut(s)	241, 703, 1702, 2513, 2653, 2832, 3000
BstSFI	CTRYAG	3 cut(s)	1068, 1140, 1300
BstSLI	GKGCMC	3 cut(s)	1183, 1205, 3089
BstV1I	GCAGC	7 cut(s)	549, 873, 1286, 2026, 2233, 2332, 2430
BstV2I	GAAGAC	1 cut(s)	241
BstX2I	RGATCY	6 cut(s)	181, 663, 1170, 1360, 1483, 2929
BstXI	CCANNNNNNTGG	1 cut(s)	1218
BstYI	RGATCY	6 cut(s)	181, 663, 1170, 1360, 1483, 2929
BstZ17I	GTATAC	1 cut(s)	2472
BsuRI	GGCC	5 cut(s)	241, 1941, 2429, 3000, 3112
BtrI	CACGTC	1 cut(s)	1324
BtsCI	GGATG	5 cut(s)	214, 958, 2408, 2749, 3040
BtsI	GCAGTG	3 cut(s)	1619, 2210, 2248
BveI	ACCTGC	1 cut(s)	3029
Cac8I	GCNNGC	6 cut(s)	194, 334, 2217, 2301, 2448, 2599
CaiI	CAGNNNCTG	1 cut(s)	1621
CfoI	GCGC	1 cut(s)	1591
Cfr10I	RCCGGY	1 cut(s)	2801
Cfr13I	GGNCC	3 cut(s)	1850, 2363, 3041
CseI	GACGC	2 cut(s)	152, 235
Csp6I	GTAC	4 cut(s)	733, 1131, 1234, 3131
CspAI	ACCGGT	1 cut(s)	2801
CspCI	CAANNNNNGTGG	4 cut(s)	1204, 1239, 1426, 1461
CviQI	GTAC	4 cut(s)	733, 1131, 1234, 3131
DraIII	CACNNNGTG	2 cut(s)	755, 1803
DrdI	GACNNNNNNGTC	2 cut(s)	640, 2170
DseDI	GACNNNNNNGTC	2 cut(s)	640, 2170
EaeI	YGGCCR	2 cut(s)	1939, 2427
Eam1104I	CTCTTC	5 cut(s)	28, 897, 2112, 2314, 2947
EarI	CTCTTC	5 cut(s)	28, 897, 2112, 2314, 2947
Ecl136II	GAGCTC	1 cut(s)	1329
Eco130I	CCWWGG	4 cut(s)	1184, 2314, 2698, 2734
Eco147I	AGGCCT	1 cut(s)	3112
Eco24I	GRGCYC	1 cut(s)	1331
Eco32I	GATATC	2 cut(s)	1393, 2649
Eco47I	GGWCC	3 cut(s)	1850, 2363, 3041
Eco53kI	GAGCTC	1 cut(s)	1329
Eco57I	CTGAAG	2 cut(s)	98, 3134
Eco88I	CYCGRG	2 cut(s)	267, 989
EcoICRI	GAGCTC	1 cut(s)	1329
EcoO109I	RGGNCCY	1 cut(s)	3041
EcoRI	GAATTC	1 cut(s)	3011
EcoRII	CCWGG	4 cut(s)	241, 703, 1702, 2653
EcoRV	GATATC	2 cut(s)	1393, 2649
EcoT14I	CCWWGG	4 cut(s)	1184, 2314, 2698, 2734
EcoT22I	ATGCAT	3 cut(s)	1551, 1666, 1831
EcoT38I	GRGCYC	1 cut(s)	1331
ErhI	CCWWGG	4 cut(s)	1184, 2314, 2698, 2734
FalI	AAGNNNNNCTT	2 cut(s)	2999, 3031
FaqI	GGGAC	3 cut(s)	445, 709, 3027
FauI	CCCGC	2 cut(s)	429, 808
FbaI	TGATCA	1 cut(s)	1245
FblI	GTMKAC	2 cut(s)	2471, 2510
Fnu4HI	GCNGC	8 cut(s)	356, 563, 862, 1300, 2015, 2247, 2346, 2444
FokI	GGATG	5 cut(s)	221, 965, 2395, 2736, 3047
FriOI	GRGCYC	1 cut(s)	1331
Fsp4HI	GCNGC	8 cut(s)	356, 563, 862, 1300, 2015, 2247, 2346, 2444
FspBI	CTAG	8 cut(s)	495, 659, 1055, 1284, 2004, 2598, 3108, 3166
FspI	TGCGCA	1 cut(s)	1590
GlaI	GCGC	1 cut(s)	1590
GluI	GCNGC	8 cut(s)	356, 563, 862, 1300, 2015, 2247, 2346, 2444
GsaI	CCCAGC	6 cut(s)	208, 414, 681, 771, 998, 3027
GsuI	CTGGAG	5 cut(s)	48, 1106, 1695, 1713, 2921
HaeIII	GGCC	5 cut(s)	241, 1941, 2429, 3000, 3112
HapII	CCGG	4 cut(s)	2515, 2802, 2834, 3001
HgaI	GACGC	2 cut(s)	152, 235
HhaI	GCGC	1 cut(s)	1591
Hin1I	GRCGYC	1 cut(s)	246
Hin6I	GCGC	1 cut(s)	1589
HinP1I	GCGC	1 cut(s)	1589
HincII	GTYRAC	2 cut(s)	2556, 3094
HindII	GTYRAC	2 cut(s)	2556, 3094
HindIII	AAGCTT	3 cut(s)	80, 794, 1685
HpaII	CCGG	4 cut(s)	2515, 2802, 2834, 3001
HphI	GGTGA	6 cut(s)	497, 635, 1931, 2343, 2643, 3010
Hpy166II	GTNNAC	9 cut(s)	691, 733, 1075, 2210, 2472, 2511, 2556, 3087, 3094
Hpy8I	GTNNAC	9 cut(s)	691, 733, 1075, 2210, 2472, 2511, 2556, 3087, 3094
Hpy99I	CGWCG	2 cut(s)	190, 3138
HpyCH4III	ACNGT	6 cut(s)	122, 1069, 2172, 2530, 2712, 3091
HpyCH4IV	ACGT	4 cut(s)	875, 1323, 1958, 2816
HpySE526I	ACGT	4 cut(s)	875, 1323, 1958, 2816
Hsp92I	GRCGYC	1 cut(s)	246
HspAI	GCGC	1 cut(s)	1589
Ksp22I	TGATCA	1 cut(s)	1245
LguI	GCTCTTC	1 cut(s)	2112
LmnI	GCTCC	6 cut(s)	381, 423, 779, 1087, 1348, 2627
Lsp1109I	GCAGC	7 cut(s)	549, 873, 1286, 2026, 2233, 2332, 2430
LweI	GCATC	9 cut(s)	155, 811, 1816, 1838, 2417, 2432, 2616, 2758, 3025
MaeI	CTAG	8 cut(s)	495, 659, 1055, 1284, 2004, 2598, 3108, 3166
MaeII	ACGT	4 cut(s)	875, 1323, 1958, 2816
MaeIII	GTNAC	5 cut(s)	1106, 1706, 2524, 2761, 2904
MfeI	CAATTG	2 cut(s)	1216, 2397
MflI	RGATCY	6 cut(s)	181, 663, 1170, 1360, 1483, 2929
MhlI	GDGCHC	4 cut(s)	1183, 1205, 1331, 3089
MlsI	TGGCCA	1 cut(s)	1941
MluNI	TGGCCA	1 cut(s)	1941
MlyI	GAGTC	3 cut(s)	1070, 2782, 3089
MmeI	TCCRAC	4 cut(s)	930, 1510, 1883, 2668
Mox20I	TGGCCA	1 cut(s)	1941
Mph1103I	ATGCAT	3 cut(s)	1551, 1666, 1831
MroXI	GAANNNNTTC	2 cut(s)	2387, 3066
MscI	TGGCCA	1 cut(s)	1941
MslI	CAYNNNNRTG	1 cut(s)	2479
Msp20I	TGGCCA	1 cut(s)	1941
MspA1I	CMGCKG	3 cut(s)	917, 1398, 3023
MspCI	CTTAAG	1 cut(s)	3140
MspI	CCGG	4 cut(s)	2515, 2802, 2834, 3001
MspR9I	CCNGG	7 cut(s)	243, 705, 1704, 2515, 2655, 2834, 3002
MunI	CAATTG	2 cut(s)	1216, 2397
Mva1269I	GAATGC	2 cut(s)	1102, 1664
MvaI	CCWGG	4 cut(s)	243, 705, 1704, 2655
NciI	CCSGG	3 cut(s)	2515, 2834, 3002
NheI	GCTAGC	1 cut(s)	2597
NlaIV	GGNNCC	9 cut(s)	183, 781, 1182, 1307, 1485, 2550, 2696, 2931, 3043
NmeAIII	GCCGAG	1 cut(s)	2879
NmuCI	GTSAC	2 cut(s)	1106, 2761
NsbI	TGCGCA	1 cut(s)	1590
NsiI	ATGCAT	3 cut(s)	1551, 1666, 1831
NspI	RCATGY	3 cut(s)	44, 336, 1912
PaeI	GCATGC	1 cut(s)	336
PceI	AGGCCT	1 cut(s)	3112
PciI	ACATGT	2 cut(s)	40, 1908
PciSI	GCTCTTC	1 cut(s)	2112
PctI	GAATGC	2 cut(s)	1102, 1664
PdmI	GAANNNNTTC	2 cut(s)	2387, 3066
PflFI	GACNNNGTC	1 cut(s)	1293
PflMI	CCANNNNNTGG	1 cut(s)	2495
PinAI	ACCGGT	1 cut(s)	2801
PkrI	GCNGC	8 cut(s)	357, 564, 863, 1301, 2016, 2248, 2347, 2445
PleI	GAGTC	3 cut(s)	1070, 2782, 3089
PpsI	GAGTC	3 cut(s)	1070, 2782, 3089
PpuMI	RGGWCCY	1 cut(s)	3041
PscI	ACATGT	2 cut(s)	40, 1908
Psp124BI	GAGCTC	1 cut(s)	1331
Psp1406I	AACGTT	1 cut(s)	2816
Psp5II	RGGWCCY	1 cut(s)	3041
Psp6I	CCWGG	4 cut(s)	241, 703, 1702, 2653
PspFI	CCCAGC	6 cut(s)	204, 410, 677, 767, 994, 3023
PspGI	CCWGG	4 cut(s)	241, 703, 1702, 2653
PspN4I	GGNNCC	9 cut(s)	183, 781, 1182, 1307, 1485, 2550, 2696, 2931, 3043
PspPI	GGNCC	3 cut(s)	1850, 2363, 3041
PspPPI	RGGWCCY	1 cut(s)	3041
PstI	CTGCAG	1 cut(s)	1304
PstNI	CAGNNNCTG	1 cut(s)	1621
PsuI	RGATCY	6 cut(s)	181, 663, 1170, 1360, 1483, 2929
PsyI	GACNNNGTC	1 cut(s)	1293
PvuII	CAGCTG	1 cut(s)	917
RsaI	GTAC	4 cut(s)	734, 1132, 1235, 3132
RsaNI	GTAC	4 cut(s)	733, 1131, 1234, 3131
RseI	CAYNNNNRTG	1 cut(s)	2479
SacI	GAGCTC	1 cut(s)	1331
SapI	GCTCTTC	1 cut(s)	2112
SatI	GCNGC	8 cut(s)	356, 563, 862, 1300, 2015, 2247, 2346, 2444
Sau96I	GGNCC	3 cut(s)	1850, 2363, 3041
ScaI	AGTACT	1 cut(s)	1235
SchI	GAGTC	3 cut(s)	1070, 2782, 3089
ScrFI	CCNGG	7 cut(s)	243, 705, 1704, 2515, 2655, 2834, 3002
SduI	GDGCHC	4 cut(s)	1183, 1205, 1331, 3089
SfaNI	GCATC	9 cut(s)	155, 811, 1816, 1838, 2417, 2432, 2616, 2758, 3025
SfcI	CTRYAG	3 cut(s)	1068, 1140, 1300
SinI	GGWCC	3 cut(s)	1850, 2363, 3041
SmiMI	CAYNNNNRTG	1 cut(s)	2479
SmlI	CTYRAG	5 cut(s)	158, 536, 797, 2502, 3140
SmoI	CTYRAG	5 cut(s)	158, 536, 797, 2502, 3140
SpeI	ACTAGT	1 cut(s)	1054
SphI	GCATGC	1 cut(s)	336
SseBI	AGGCCT	1 cut(s)	3112
SsiI	CCGC	8 cut(s)	356, 422, 572, 728, 815, 1287, 1398, 3021
SspI	AATATT	2 cut(s)	1430, 2128
SspMI	CTAG	8 cut(s)	495, 659, 1055, 1284, 2004, 2598, 3108, 3166
SstI	GAGCTC	1 cut(s)	1331
StuI	AGGCCT	1 cut(s)	3112
StyD4I	CCNGG	7 cut(s)	241, 703, 1702, 2513, 2653, 2832, 3000
StyI	CCWWGG	4 cut(s)	1184, 2314, 2698, 2734
TaaI	ACNGT	6 cut(s)	122, 1069, 2172, 2530, 2712, 3091
TaiI	ACGT	4 cut(s)	878, 1326, 1961, 2819
TaqI	TCGA	3 cut(s)	320, 548, 959
TaqII	GACCGA	1 cut(s)	16
TatI	WGTACW	2 cut(s)	732, 1233
TauI	GCSGC	1 cut(s)	358
TseFI	GTSAC	2 cut(s)	1106, 2761
TseI	GCWGC	7 cut(s)	562, 861, 1299, 2014, 2246, 2345, 2443
Tsp45I	GTSAC	2 cut(s)	1106, 2761
TspDTI	ATGAA	6 cut(s)	700, 821, 969, 1355, 2249, 2705
TspGWI	ACGGA	5 cut(s)	174, 1476, 1989, 2491, 2541
Tth111I	GACNNNGTC	1 cut(s)	1293
Van91I	CCANNNNNTGG	1 cut(s)	2495
Vha464I	CTTAAG	1 cut(s)	3140
VneI	GTGCAC	1 cut(s)	3085
VpaK11BI	GGWCC	3 cut(s)	1850, 2363, 3041
XceI	RCATGY	3 cut(s)	44, 336, 1912
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2321
XmiI	GTMKAC	2 cut(s)	2471, 2510
XmnI	GAANNNNTTC	2 cut(s)	2387, 3066
XspI	CTAG	8 cut(s)	495, 659, 1055, 1284, 2004, 2598, 3108, 3166
ZrmI	AGTACT	1 cut(s)	1235
Zsp2I	ATGCAT	3 cut(s)	1551, 1666, 1831

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.