Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0003396

Physical Location & Seq

Genomic Coordinates Reverse (-)

5364239 .. 5368468

Gene Structure 4230 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1076000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2061 bp

ATGAAAGGGGAGGGGAAGATGGTTCTGAAGTCGAAGATGAAATGGGTTGGACTGGTTGGACTGTTTCTCTCAGCTATCTCTCTCTTTGTGCACTTTCTGCTGGCCAGATTCACAGAAGGGGCTGCTGCTTCAGAGTTCCACTCCTCCATCACAATCTTCCCCAGAAGAGCCATTGGTGAAAGCTTGAATTTTTCCAAGACTAGCCCAATGTATAGAAGGCTATGGGGTCCGGTTCGGCATCTCGAATCTTTGCAACCGAATGCAAATCCCAGAGGACATTATGCCGATCCTGGTTCAGATACCAACGGGTTTATCTTTGTCAGGATACAAGGCGGATTTCATGAGATCAGGAATTCGATATGTGATGTGGTTGTGGTTTCTCGGTTTCTTAATGCAACGTTAGTGATTCCTGAGATCCAATCAACCACGAGCAGCAAGGGCATCAGCTCTCAGTTTAAGAGTTTTGCCTATCTCTACAATGAGGACCAATTCATGGCAGCCTTGTCAAAAGACGTCAAAGTTGTGAAGACGCTTCCCAAAAATCTCAAGGGGCAAAGGAGAAAAAAGAACATTCCGGTGTTTAAAGTGCCTTACTCTGCTTCTCCGTATTACTATAAGAACCATGTTCTTCCAGTTTTGAAGAAGCATTTAGTAGTTGAACTAGTTGTGTCTGACGGTGGATGCTTGCAGGCTATCCTGCCACCCGATCTTGAAGAATATCAGAGGCTGAGATGTAGGGTTGCTTTTCATGCTCTTAGGTTCCGGCAGGAGGTCCAGGAACTTGCCACCAAAATTTTACAAAGATTAAAGGCTCCTGGACAGCCATTCATTGCATTTGACCCTGGAATGACCCGGGAGGCTTTGGCATTTCATGGTTGTGCTGAACTCTTCCAGGATGTACACACTGAACTCATTCAGCACAAAAGACAATGGATGCTAAAACGTGGAATTGTCAAGGGAAAGCTTTCCGTGAATTCTGCAGAACAGCGCCTTAAAGGTTCTTGCCCACTGATGCCGGAAGAGATTGGTATTATTCTTCGTGCGTATGGGTACTTGTGGGACACAATAATATATGTCTCAGGGGGAGAAATCTTTGGTGGCCAAAGGACATTGATACCTCTTCGTGCAATGTTTGAAAATGTCGTTGATAGGACTTCCCTCAGCATGCCTTGGGAGCTTGGTAGGATGTATGGTCGCGAAGCTAACCTTGTTGATCGCAATCCCAGGAGTCCACCGATGATTAAGGAAGAGATGAAAATGGAAGCATGGAAAAATGCAGGCCCACGCCCTCGCCCACTTCCACCACCCCCGGCCAGGCCCAAATTCTATAACATTGAAGGTTGGTGGGGTTGGGTAGCTGAGAGTGATAACGAGCCTGATAGTACAGTTATCGAATTAAGGACCAATGCTCATAAACTTCTGTGGGAAGCAATTGATTATGTGATATGCGTTGAAGCTGATGTCTTTATCCCTGGATTCGATATTGATGGTAAGGGACACCCAAATTTTGCAAGCTTGGTAATGGGACACCGACTCTATCAATCAGCTGAATCAAAGACATACAGACCAGACAGAAAAGAAGTCGGCAAGCTTTTACATGAAATTCATGACCACCTATACCATGCAAACCATACTTGGCTGATGTCAGTACGCAAGCATTTGAGGAAAAGATTAGTTGATGGATTAATAGAAGCTTCAGCAAGATCAAAACCACTGTCTTTTATTTCTCATCCAATTCCGGAATGTTCTTGCTTAAGGCACAATCCTGCTGGAAAATCAATTAATGCCACGTCGTCACCTCATGCTTCAACTCCTAGTCATTCTCAAGTTCTTTCCGCTCTTGGAGTTGTGCACTATTGCCCTGCTTGGATGGAGAATGATTTGACATTGCAGTCAAAAGACAAGGAAAATGAAGAAAATCTAGATGAAGACGACTCCACCTCATCAGGATTGTTCTTCCGGAAAAATAATGGAGATCATGACGGTGGTAATGGAGACATAAGCAACAAAGAAGAAACTCAGTTGGAGGATCAAGAAGAGCTCGAGGGTGGAGAACAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0012505 GO:0031410 GO:0031982 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

O-FucT

Protein Analysis

687

Amino Acids

77.58

Weight (kDa)

8.71

Isoelectric Point (pI)

50.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
O-FucT	PF10250	104 - 405	1.7e-68	GDP-fucose protein O-fucosyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003396)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G30300 AT3G30300
fragaria_vesca	FvH4_4g06281 FvH4_6g46210 FvH4_6g46210 FvH4_6g46210
malus_domestica	MD09G1076000.v1.1 MD17G1066700.v1.1
prunus_persica	Prupe.3G248000_v2.0.a1
pyrus_communis	pycom09g00340
rosa_chinensis	RchiOBHm_Chr2g0164661 RchiOBHm_Chr6g0258841 RchiOBHm_Chr6g0258851
rosa_laevigata	RLG00000014579 RLG00000021475
rosa_roxburghii	Rroxscaffold_2G00086400 Rroxscaffold_3G00256120 Rroxscaffold_3G00256130 Rroxscaffold_5G00351080 Rroxscaffold_7G00206110
rosa_rugosa	Rorug02G0509600 Rorug05G0583900.1
rosa_samantha	Rh1AG019300 Rh1CG016900 Rh1DG013900 Rh2AG577500 Rh2BG588700 Rh2CG559500 Rh2DG599300 Rh6AG100300 Rh6CG089300 Rh6DG083600 Rh6DG083700
rosa_wichuraiana	Rw1G001260 Rw2G047960 Rw6G008690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1891
AatII	GACGTC	1 cut(s)	516
AccB7I	CCANNNNNTGG	1 cut(s)	493
AccBSI	CCGCTC	1 cut(s)	1838
AccII	CGCG	1 cut(s)	1197
AccIII	TCCGGA	2 cut(s)	1738, 1959
AciI	CCGC	2 cut(s)	333, 1836
AclI	AACGTT	1 cut(s)	398
AclWI	GGATC	3 cut(s)	281, 409, 2037
AcoI	YGGCCR	3 cut(s)	102, 1099, 1311
AcsI	RAATTY	7 cut(s)	187, 352, 792, 973, 1322, 1504, 1602
AcuI	CTGAAG	3 cut(s)	47, 114, 1680
AcyI	GRCGYC	1 cut(s)	513
AfaI	GTAC	4 cut(s)	900, 1052, 1384, 1650
AfiI	CCNNNNNNNGG	3 cut(s)	493, 769, 1314
AflII	CTTAAG	1 cut(s)	1753
AgsI	TTSAA	8 cut(s)	187, 640, 659, 713, 1136, 1337, 1454, 1809
AhlI	ACTAGT	1 cut(s)	661
AjiI	CACGTC	1 cut(s)	1791
AjnI	CCWGG	8 cut(s)	289, 774, 814, 841, 891, 1223, 1313, 1471
Alw21I	GWGCWC	3 cut(s)	93, 1854, 2043
Alw26I	GTCTC	2 cut(s)	1081, 1989
Alw44I	GTGCAC	2 cut(s)	89, 1850
AlwI	GGATC	3 cut(s)	281, 409, 2037
AlwNI	CAGNNNCTG	1 cut(s)	727
Ama87I	CYCGRG	2 cut(s)	852, 2042
Aor13HI	TCCGGA	2 cut(s)	1738, 1959
AoxI	GGCC	5 cut(s)	102, 1099, 1279, 1311, 1316
ApaLI	GTGCAC	2 cut(s)	89, 1850
ApeKI	GCWGC	4 cut(s)	122, 125, 432, 497
ApoI	RAATTY	7 cut(s)	187, 352, 792, 973, 1322, 1504, 1602
ArsI	GACNNNNNNTTYG	2 cut(s)	1700, 1732
AseI	ATTAAT	2 cut(s)	1685, 1782
Asp700I	GAANNNNTTC	2 cut(s)	912, 964
AspLEI	GCGC	1 cut(s)	990
AspS9I	GGNCC	6 cut(s)	227, 484, 772, 1280, 1317, 1401
AsuC2I	CCSGG	3 cut(s)	853, 854, 1310
AsuHPI	GGTGA	2 cut(s)	188, 1788
AvaI	CYCGRG	2 cut(s)	852, 2042
AvaII	GGWCC	4 cut(s)	227, 484, 772, 1401
BaeGI	GKGCMC	2 cut(s)	93, 1854
BalI	TGGCCA	2 cut(s)	104, 1101
BanII	GRGCYC	1 cut(s)	2043
BauI	CACGAG	1 cut(s)	427
BbsI	GAAGAC	2 cut(s)	533, 1935
Bbv12I	GWGCWC	3 cut(s)	93, 1854, 2043
BbvCI	CCTCAGC	1 cut(s)	1160
BbvI	GCAGC	4 cut(s)	109, 112, 444, 509
BccI	CCATC	5 cut(s)	13, 155, 1481, 1673, 1864
BciT130I	CCWGG	8 cut(s)	291, 776, 816, 843, 893, 1225, 1315, 1473
BciVI	GTATCC	1 cut(s)	318
BcnI	CCSGG	3 cut(s)	853, 854, 1310
BcoDI	GTCTC	2 cut(s)	1081, 1989
BcuI	ACTAGT	1 cut(s)	661
BfaI	CTAG	4 cut(s)	201, 662, 1815, 1922
BfmI	CTRYAG	1 cut(s)	978
BfoI	RGCGCY	1 cut(s)	991
BfrI	CTTAAG	1 cut(s)	1753
BfuI	GTATCC	1 cut(s)	318
BisI	GCNGC	4 cut(s)	123, 126, 433, 498
BlsI	GCNGC	4 cut(s)	124, 127, 434, 499
Bme18I	GGWCC	4 cut(s)	227, 484, 772, 1401
BmeT110I	CYCGRG	2 cut(s)	852, 2042
BmgBI	CACGTC	1 cut(s)	1791
BmgT120I	GGNCC	6 cut(s)	227, 484, 772, 1280, 1317, 1401
BmiI	GGNNCC	3 cut(s)	228, 761, 813
BmsI	GCATC	5 cut(s)	247, 450, 671, 924, 1002
BpiI	GAAGAC	2 cut(s)	533, 1935
BplI	GAGNNNNNCTC	2 cut(s)	125, 157
Bpu10I	CCTNAGC	1 cut(s)	1160
BpuEI	CTTGAG	2 cut(s)	530, 1809
BpuMI	CCSGG	3 cut(s)	853, 854, 1310
BsaBI	GATNNNNATC	1 cut(s)	1218
BsaHI	GRCGYC	1 cut(s)	513
BsaJI	CCNNGG	6 cut(s)	841, 852, 1169, 1223, 1308, 1471
BsaWI	WCCGGW	4 cut(s)	229, 574, 1738, 1959
Bsc4I	CCNNNNNNNGG	3 cut(s)	493, 769, 1314
Bse1I	ACTGG	2 cut(s)	57, 632
Bse3DI	GCAATG	3 cut(s)	828, 1134, 1886
Bse8I	GATNNNNATC	1 cut(s)	1218
BseAI	TCCGGA	2 cut(s)	1738, 1959
BseBI	CCWGG	8 cut(s)	291, 776, 816, 843, 893, 1225, 1315, 1473
BseDI	CCNNGG	6 cut(s)	841, 852, 1169, 1223, 1308, 1471
BseGI	GGATG	6 cut(s)	686, 901, 939, 1191, 1729, 1875
BseJI	GATNNNNATC	1 cut(s)	1218
BseLI	CCNNNNNNNGG	3 cut(s)	493, 769, 1314
BseMI	GCAATG	3 cut(s)	828, 1134, 1886
BseMII	CTCAG	8 cut(s)	84, 402, 464, 719, 1092, 1174, 1350, 2033
BseNI	ACTGG	2 cut(s)	57, 632
BseRI	GAGGAG	1 cut(s)	133
BseSI	GKGCMC	2 cut(s)	93, 1854
BseXI	GCAGC	4 cut(s)	109, 112, 444, 509
Bsh1236I	CGCG	1 cut(s)	1197
BshFI	GGCC	5 cut(s)	104, 1101, 1281, 1313, 1318
BsiHKAI	GWGCWC	3 cut(s)	93, 1854, 2043
BsiHKCI	CYCGRG	2 cut(s)	852, 2042
BsiSI	CCGG	8 cut(s)	230, 575, 763, 853, 1016, 1310, 1739, 1960
BslFI	GGGAC	3 cut(s)	1073, 1509, 1539
BslI	CCNNNNNNNGG	3 cut(s)	493, 769, 1314
BsmAI	GTCTC	2 cut(s)	1081, 1989
BsmFI	GGGAC	3 cut(s)	1073, 1509, 1539
BsmI	GAATGC	1 cut(s)	265
BsnI	GGCC	5 cut(s)	104, 1101, 1281, 1313, 1318
BsoBI	CYCGRG	2 cut(s)	852, 2042
Bsp1286I	GDGCHC	3 cut(s)	93, 1854, 2043
Bsp13I	TCCGGA	2 cut(s)	1738, 1959
Bsp1407I	TGTACA	1 cut(s)	898
Bsp143I	GATC	8 cut(s)	286, 345, 414, 706, 1213, 1703, 1975, 2029
Bsp68I	TCGCGA	1 cut(s)	1197
BspACI	CCGC	2 cut(s)	333, 1836
BspANI	GGCC	5 cut(s)	104, 1101, 1281, 1313, 1318
BspCNI	CTCAG	8 cut(s)	83, 403, 463, 720, 1091, 1173, 1351, 2032
BspEI	TCCGGA	2 cut(s)	1738, 1959
BspFNI	CGCG	1 cut(s)	1197
BspHI	TCATGA	3 cut(s)	340, 1606, 1978
BspLI	GGNNCC	3 cut(s)	228, 761, 813
BspMAI	CTGCAG	1 cut(s)	982
BspPI	GGATC	3 cut(s)	281, 409, 2037
BspQI	GCTCTTC	2 cut(s)	160, 2031
BspTI	CTTAAG	1 cut(s)	1753
BsrBI	CCGCTC	1 cut(s)	1838
BsrDI	GCAATG	3 cut(s)	828, 1134, 1886
BsrGI	TGTACA	1 cut(s)	898
BsrI	ACTGG	2 cut(s)	57, 632
BssECI	CCNNGG	6 cut(s)	841, 852, 1169, 1223, 1308, 1471
BssMI	GATC	8 cut(s)	286, 345, 414, 706, 1213, 1703, 1975, 2029
BssNI	GRCGYC	1 cut(s)	513
BssSI	CACGAG	1 cut(s)	427
BssT1I	CCWWGG	1 cut(s)	1169
Bst2BI	CACGAG	1 cut(s)	427
Bst2UI	CCWGG	8 cut(s)	291, 776, 816, 843, 893, 1225, 1315, 1473
Bst4CI	ACNGT	6 cut(s)	63, 677, 1387, 1716, 1985, 2058
Bst6I	CTCTTC	6 cut(s)	160, 893, 1014, 1125, 1242, 2031
BstACI	GRCGYC	1 cut(s)	513
BstAFI	CTTAAG	1 cut(s)	1753
BstAPI	GCANNNNNTGC	1 cut(s)	97
BstAUI	TGTACA	1 cut(s)	898
BstC8I	GCNNGC	8 cut(s)	102, 686, 690, 1166, 1279, 1513, 1589, 1655
BstDEI	CTNAG	9 cut(s)	70, 411, 450, 728, 755, 1078, 1160, 1359, 2019
BstF5I	GGATG	6 cut(s)	686, 901, 939, 1191, 1729, 1875
BstFNI	CGCG	1 cut(s)	1197
BstH2I	RGCGCY	1 cut(s)	991
BstHHI	GCGC	1 cut(s)	990
BstKTI	GATC	8 cut(s)	289, 348, 417, 709, 1216, 1706, 1978, 2032
BstMAI	GTCTC	2 cut(s)	1081, 1989
BstMBI	GATC	8 cut(s)	286, 345, 414, 706, 1213, 1703, 1975, 2029
BstMWI	GCNNNNNNNGC	4 cut(s)	97, 438, 749, 1174
BstNI	CCWGG	8 cut(s)	291, 776, 816, 843, 893, 1225, 1315, 1473
BstNSI	RCATGY	1 cut(s)	1168
BstSFI	CTRYAG	1 cut(s)	978
BstSLI	GKGCMC	2 cut(s)	93, 1854
BstUI	CGCG	1 cut(s)	1197
BstV1I	GCAGC	4 cut(s)	109, 112, 444, 509
BstV2I	GAAGAC	2 cut(s)	533, 1935
BstX2I	RGATCY	1 cut(s)	414
BstYI	RGATCY	1 cut(s)	414
BsuI	GTATCC	1 cut(s)	318
BsuRI	GGCC	5 cut(s)	104, 1101, 1281, 1313, 1318
BtrI	CACGTC	1 cut(s)	1791
BtsCI	GGATG	6 cut(s)	686, 901, 939, 1191, 1729, 1875
BtsIMutI	CAGTG	3 cut(s)	903, 1007, 1712
BtuMI	TCGCGA	1 cut(s)	1197
Cac8I	GCNNGC	8 cut(s)	102, 686, 690, 1166, 1279, 1513, 1589, 1655
CaiI	CAGNNNCTG	1 cut(s)	727
CciI	TCATGA	3 cut(s)	340, 1606, 1978
CfoI	GCGC	1 cut(s)	990
Cfr13I	GGNCC	6 cut(s)	227, 484, 772, 1280, 1317, 1401
Cfr9I	CCCGGG	1 cut(s)	852
CseI	GACGC	1 cut(s)	538
Csp6I	GTAC	4 cut(s)	899, 1051, 1383, 1649
CviQI	GTAC	4 cut(s)	899, 1051, 1383, 1649
DdeI	CTNAG	9 cut(s)	70, 411, 450, 728, 755, 1078, 1160, 1359, 2019
DpnI	GATC	8 cut(s)	288, 347, 416, 708, 1215, 1705, 1977, 2031
DpnII	GATC	8 cut(s)	286, 345, 414, 706, 1213, 1703, 1975, 2029
DraI	TTTAAA	1 cut(s)	583
DrdI	GACNNNNNNGTC	1 cut(s)	1891
DseDI	GACNNNNNNGTC	1 cut(s)	1891
EaeI	YGGCCR	3 cut(s)	102, 1099, 1311
Eam1104I	CTCTTC	6 cut(s)	160, 893, 1014, 1125, 1242, 2031
EarI	CTCTTC	6 cut(s)	160, 893, 1014, 1125, 1242, 2031
EciI	GGCGGA	1 cut(s)	348
Ecl136II	GAGCTC	1 cut(s)	2041
Eco130I	CCWWGG	1 cut(s)	1169
Eco24I	GRGCYC	1 cut(s)	2043
Eco47I	GGWCC	4 cut(s)	227, 484, 772, 1401
Eco53kI	GAGCTC	1 cut(s)	2041
Eco57I	CTGAAG	3 cut(s)	47, 114, 1680
Eco88I	CYCGRG	2 cut(s)	852, 2042
EcoICRI	GAGCTC	1 cut(s)	2041
EcoRI	GAATTC	2 cut(s)	352, 973
EcoRII	CCWGG	8 cut(s)	289, 774, 814, 841, 891, 1223, 1313, 1471
EcoT14I	CCWWGG	1 cut(s)	1169
EcoT38I	GRGCYC	1 cut(s)	2043
ErhI	CCWWGG	1 cut(s)	1169
FalI	AAGNNNNNCTT	2 cut(s)	1191, 1223
FaqI	GGGAC	3 cut(s)	1073, 1509, 1539
Fnu4HI	GCNGC	4 cut(s)	123, 126, 433, 498
FokI	GGATG	6 cut(s)	693, 908, 946, 1198, 1716, 1882
FriOI	GRGCYC	1 cut(s)	2043
Fsp4HI	GCNGC	4 cut(s)	123, 126, 433, 498
FspBI	CTAG	4 cut(s)	201, 662, 1815, 1922
GlaI	GCGC	1 cut(s)	989
GluI	GCNGC	4 cut(s)	123, 126, 433, 498
HaeII	RGCGCY	1 cut(s)	991
HaeIII	GGCC	5 cut(s)	104, 1101, 1281, 1313, 1318
HapII	CCGG	8 cut(s)	230, 575, 763, 853, 1016, 1310, 1739, 1960
HgaI	GACGC	1 cut(s)	538
HhaI	GCGC	1 cut(s)	990
Hin1I	GRCGYC	1 cut(s)	513
Hin6I	GCGC	1 cut(s)	988
HinP1I	GCGC	1 cut(s)	988
HindIII	AAGCTT	5 cut(s)	181, 962, 1513, 1589, 1692
HinfI	GANTC	8 cut(s)	108, 245, 406, 1228, 1476, 1533, 1550, 1934
HpaII	CCGG	8 cut(s)	230, 575, 763, 853, 1016, 1310, 1739, 1960
HphI	GGTGA	2 cut(s)	188, 1788
Hpy166II	GTNNAC	4 cut(s)	91, 901, 1232, 1852
Hpy188I	TCNGA	5 cut(s)	27, 133, 298, 673, 723
Hpy8I	GTNNAC	4 cut(s)	91, 901, 1232, 1852
Hpy99I	CGWCG	1 cut(s)	1795
HpyAV	CCTTC	3 cut(s)	110, 210, 1331
HpyCH4III	ACNGT	6 cut(s)	63, 677, 1387, 1716, 1985, 2058
HpyCH4IV	ACGT	4 cut(s)	398, 513, 943, 1790
HpyF10VI	GCNNNNNNNGC	4 cut(s)	97, 438, 749, 1174
HpyF3I	CTNAG	9 cut(s)	70, 411, 450, 728, 755, 1078, 1160, 1359, 2019
HpySE526I	ACGT	4 cut(s)	398, 513, 943, 1790
Hsp92I	GRCGYC	1 cut(s)	513
HspAI	GCGC	1 cut(s)	988
Kpn2I	TCCGGA	2 cut(s)	1738, 1959
Kzo9I	GATC	8 cut(s)	286, 345, 414, 706, 1213, 1703, 1975, 2029
LguI	GCTCTTC	2 cut(s)	160, 2031
LmnI	GCTCC	2 cut(s)	817, 1174
Lsp1109I	GCAGC	4 cut(s)	109, 112, 444, 509
LweI	GCATC	5 cut(s)	247, 450, 671, 924, 1002
MaeI	CTAG	4 cut(s)	201, 662, 1815, 1922
MaeII	ACGT	4 cut(s)	398, 513, 943, 1790
MaeIII	GTNAC	1 cut(s)	1794
MalI	GATC	8 cut(s)	288, 347, 416, 708, 1215, 1705, 1977, 2031
MbiI	CCGCTC	1 cut(s)	1838
MboI	GATC	8 cut(s)	286, 345, 414, 706, 1213, 1703, 1975, 2029
MfeI	CAATTG	1 cut(s)	1431
MflI	RGATCY	1 cut(s)	414
MhlI	GDGCHC	3 cut(s)	93, 1854, 2043
MlsI	TGGCCA	2 cut(s)	104, 1101
MluNI	TGGCCA	2 cut(s)	104, 1101
MlyI	GAGTC	3 cut(s)	1237, 1527, 1928
MmeI	TCCRAC	3 cut(s)	28, 37, 2004
Mox20I	TGGCCA	2 cut(s)	104, 1101
MroI	TCCGGA	2 cut(s)	1738, 1959
MroXI	GAANNNNTTC	2 cut(s)	912, 964
MscI	TGGCCA	2 cut(s)	104, 1101
MslI	CAYNNNNRTG	3 cut(s)	575, 876, 1983
Msp20I	TGGCCA	2 cut(s)	104, 1101
MspA1I	CMGCKG	1 cut(s)	1547
MspCI	CTTAAG	1 cut(s)	1753
MspI	CCGG	8 cut(s)	230, 575, 763, 853, 1016, 1310, 1739, 1960
MunI	CAATTG	1 cut(s)	1431
Mva1269I	GAATGC	1 cut(s)	265
MvaI	CCWGG	8 cut(s)	291, 776, 816, 843, 893, 1225, 1315, 1473
MvnI	CGCG	1 cut(s)	1197
MwoI	GCNNNNNNNGC	4 cut(s)	97, 438, 749, 1174
NciI	CCSGG	3 cut(s)	853, 854, 1310
NdeII	GATC	8 cut(s)	286, 345, 414, 706, 1213, 1703, 1975, 2029
NlaIV	GGNNCC	3 cut(s)	228, 761, 813
NmuCI	GTSAC	1 cut(s)	1794
NruI	TCGCGA	1 cut(s)	1197
NspI	RCATGY	1 cut(s)	1168
PaeI	GCATGC	1 cut(s)	1168
PaeR7I	CTCGAG	1 cut(s)	2042
PagI	TCATGA	3 cut(s)	340, 1606, 1978
PciSI	GCTCTTC	2 cut(s)	160, 2031
PctI	GAATGC	1 cut(s)	265
PdmI	GAANNNNTTC	2 cut(s)	912, 964
PfeI	GAWTC	5 cut(s)	108, 245, 406, 1476, 1550
PflMI	CCANNNNNTGG	1 cut(s)	493
PfoI	TCCNGGA	3 cut(s)	774, 814, 891
PkrI	GCNGC	4 cut(s)	124, 127, 434, 499
PleI	GAGTC	3 cut(s)	1236, 1527, 1928
PpsI	GAGTC	3 cut(s)	1236, 1527, 1928
PshBI	ATTAAT	2 cut(s)	1685, 1782
Psp124BI	GAGCTC	1 cut(s)	2043
Psp1406I	AACGTT	1 cut(s)	398
Psp6I	CCWGG	8 cut(s)	289, 774, 814, 841, 891, 1223, 1313, 1471
PspGI	CCWGG	8 cut(s)	289, 774, 814, 841, 891, 1223, 1313, 1471
PspN4I	GGNNCC	3 cut(s)	228, 761, 813
PspPI	GGNCC	6 cut(s)	227, 484, 772, 1280, 1317, 1401
PspXI	VCTCGAGB	1 cut(s)	2042
PstI	CTGCAG	1 cut(s)	982
PstNI	CAGNNNCTG	1 cut(s)	727
PsuI	RGATCY	1 cut(s)	414
PvuII	CAGCTG	1 cut(s)	1547
RruI	TCGCGA	1 cut(s)	1197
RsaI	GTAC	4 cut(s)	900, 1052, 1384, 1650
RsaNI	GTAC	4 cut(s)	899, 1051, 1383, 1649
RseI	CAYNNNNRTG	3 cut(s)	575, 876, 1983
SacI	GAGCTC	1 cut(s)	2043
SapI	GCTCTTC	2 cut(s)	160, 2031
SatI	GCNGC	4 cut(s)	123, 126, 433, 498
Sau3AI	GATC	8 cut(s)	286, 345, 414, 706, 1213, 1703, 1975, 2029
Sau96I	GGNCC	6 cut(s)	227, 484, 772, 1280, 1317, 1401
SchI	GAGTC	3 cut(s)	1237, 1527, 1928
SduI	GDGCHC	3 cut(s)	93, 1854, 2043
SfaNI	GCATC	5 cut(s)	247, 450, 671, 924, 1002
SfcI	CTRYAG	1 cut(s)	978
Sfr274I	CTCGAG	1 cut(s)	2042
SinI	GGWCC	4 cut(s)	227, 484, 772, 1401
SlaI	CTCGAG	1 cut(s)	2042
SmaI	CCCGGG	1 cut(s)	854
SmiMI	CAYNNNNRTG	3 cut(s)	575, 876, 1983
SmlI	CTYRAG	4 cut(s)	545, 1753, 1824, 2042
SmoI	CTYRAG	4 cut(s)	545, 1753, 1824, 2042
SpeI	ACTAGT	1 cut(s)	661
SphI	GCATGC	1 cut(s)	1168
SsiI	CCGC	2 cut(s)	333, 1836
SspMI	CTAG	4 cut(s)	201, 662, 1815, 1922
SstI	GAGCTC	1 cut(s)	2043
StyI	CCWWGG	1 cut(s)	1169
TaaI	ACNGT	6 cut(s)	63, 677, 1387, 1716, 1985, 2058
TaiI	ACGT	4 cut(s)	401, 516, 946, 1793
TaqI	TCGA	6 cut(s)	32, 243, 356, 1392, 1479, 2043
TatI	WGTACW	2 cut(s)	898, 1382
TfiI	GAWTC	5 cut(s)	108, 245, 406, 1476, 1550
TscAI	CASTG	3 cut(s)	910, 1014, 1719
TseFI	GTSAC	1 cut(s)	1794
TseI	GCWGC	4 cut(s)	122, 125, 432, 497
Tsp45I	GTSAC	1 cut(s)	1794
TspGWI	ACGGA	2 cut(s)	594, 958
TspMI	CCCGGG	1 cut(s)	852
TspRI	CASTG	3 cut(s)	910, 1014, 1719
Van91I	CCANNNNNTGG	1 cut(s)	493
Vha464I	CTTAAG	1 cut(s)	1753
VneI	GTGCAC	2 cut(s)	89, 1850
VpaK11BI	GGWCC	4 cut(s)	227, 484, 772, 1401
VspI	ATTAAT	2 cut(s)	1685, 1782
XapI	RAATTY	7 cut(s)	187, 352, 792, 973, 1322, 1504, 1602
XbaI	TCTAGA	1 cut(s)	1921
XceI	RCATGY	1 cut(s)	1168
XhoI	CTCGAG	1 cut(s)	2042
XmaI	CCCGGG	1 cut(s)	852
XmnI	GAANNNNTTC	2 cut(s)	912, 964
XspI	CTAG	4 cut(s)	201, 662, 1815, 1922
ZraI	GACGTC	1 cut(s)	514

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.