Rh2AG577500

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0003396
Physical Location & Seq
Forward (+)
80723326 .. 80729198
5873 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG577500.1

Sequence Viewer

Length: 1575 bp
ATGAAAGCAGAAGGGAAGATGGTGTTGAAGTCAAAAATGAAATGGGTTGGTCTTTTTGGTCTGGTTCTCTCAGCTATTTCTCTGTTTGTTCACTTTTTACTGGCTCGATTTACTGAGGAGGGCGTTTCAGAGTTCCATTCTTCAATCACAATCTTCCCAAGGCGAACCATTGGTGAAAGCTTGAATTTTTCCAAGACTAGTGCAATGTATAGAAGGCTATGGGGTCCGGTAAGGCCTCTCGAATCATTGCATCCTGATGCAAATCCCAGAGGATATTATTCCGATCCCAATTCAGATACCAATGGTTTTATCTTTGTGAGGATACAAGGCGGGTTTCATGAGATCAGGAATTCGATATGTGATGTAGTTGTGATTTCTCGGTTTCTTAATGCAACATTAGTTATTCCTGAGCTCCAATCAACCACAAGCAGCAAGGGCATCAGCTCTCAGTTTAAGAGTTTTGCCTATCTCTACAATGAGGACCAATTCATGGCAGCATTATCAAAAGACATCAAAGTTGTGAAAACCCTCCCTAAAAATCTCAAAGGGCAAAGGAAGAAAAAAAATATTCCCTCGTTTAAAGTGCCTTACTCTGCTTCACCATACTACTATAAGCACCGCGTTCTTCCAGTTTTAAAGAAGCATTCAGTAGTTGAACTAGTTGTGTCTGACGGTGGATGCTTGCAGGCTATCCTGCCACCAGATCTTGAAGAGTACCAGAGGCTGAGATGTAGGGTTGCTTTTCATGCACTTCGGTTCCGGCAGGAGGTCCAGGAACTTGCTACCAAAATTTTACACAGATTACGGGCCCCTGGACAGCCATTTGTAGCATTTGACCCTGGAATGACCAGAGATGCTTTGGCATATCATGGTTGTGCCGAACTCTTCCAGGATGTACACACTGAACTCATTCAGCACAAAAGACAGTGGATGATAAAACGTGGGATTGTCAAGGGGAATCTTTCGGTGAATTCTGCAAAACAACGACTTAAAGGTTCTTGCCCACTAATGCCGGAAGAGATTGGTATTATTCTTCGTGCATATGGATACTTGTGGGACACAATAATATATGTGTCTGGTGGAGAAATCTTTGGTGGCCAAAGGACATTGATCCCTCTTCGTGCGATGTTTGAAAATGTTGTTGATAGGACCTCCCTCAGCATTCTCTGGGAGCTTAGTAGGATTTATGGTCGTGAGGCTAACCTCTTTGAAAGTAATCCCAGGAGTCCACCTACAGTCGAGGAAGAGATGAAGGTTGAAGCATGGAAAACTGCAGGGCCACGTCCTCGCCCACTTCCACCGCCTCCAGCCAGGCCTAAATCATATAACATTGAAGGTTGGTGGGGCTGGGTAGCTGAGAGTGATAATGAGCCTGATAGTACAGTTATGGAATTGAGGACAAACGCTCATAAACTACTGTGGGAAGCAATTGATTACGTGATATGCGTTGAAGCTGATGTATTTATCCCTGGATTTGATCGTGATGGTAAGGGACATCCAAATTTTGCAAGCTTAGTAATGGGACATAGACTCTATCAGTCAGCTGCATCAAAAACATACAGGCCAGACAGGTAA

Protein Analysis

524

Amino Acids

59.62

Weight (kDa)

9.46

Isoelectric Point (pI)

44.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 103 - 399 1.2e-69 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 490
AccII CGCG 1 cut(s) 621
AciI CCGC 3 cut(s) 330, 619, 1301
AclWI GGATC 2 cut(s) 278, 1105
AcoI YGGCCR 1 cut(s) 1096
AcsI RAATTY 5 cut(s) 184, 349, 789, 970, 1501
AfaI GTAC 3 cut(s) 716, 897, 1381
AfiI CCNNNNNNNGG 2 cut(s) 490, 766
AhlI ACTAGT 2 cut(s) 197, 658
AjiI CACGTC 1 cut(s) 1283
AjnI CCWGG 7 cut(s) 771, 811, 838, 888, 1220, 1310, 1468
AluBI AGCT 9 cut(s) 74, 180, 412, 444, 1174, 1355, 1454, 1512, 1544
AluI AGCT 9 cut(s) 74, 180, 412, 444, 1174, 1355, 1454, 1512, 1544
Alw21I GWGCWC 1 cut(s) 414
AlwI GGATC 2 cut(s) 278, 1105
AlwNI CAGNNNCTG 1 cut(s) 724
AoxI GGCC 6 cut(s) 233, 807, 1096, 1277, 1313, 1562
ApaI GGGCCC 1 cut(s) 811
ApeKI GCWGC 3 cut(s) 429, 494, 1544
ApoI RAATTY 5 cut(s) 184, 349, 789, 970, 1501
Asp700I GAANNNNTTC 1 cut(s) 909
AspS9I GGNCC 7 cut(s) 224, 481, 769, 807, 808, 1149, 1277
AsuHPI GGTGA 3 cut(s) 185, 591, 979
AvaII GGWCC 4 cut(s) 224, 481, 769, 1149
BaeGI GKGCMC 1 cut(s) 811
BalI TGGCCA 1 cut(s) 1098
BanII GRGCYC 2 cut(s) 414, 811
Bbv12I GWGCWC 1 cut(s) 414
BbvCI CCTCAGC 1 cut(s) 1157
BbvI GCAGC 3 cut(s) 441, 506, 1531
BccI CCATC 2 cut(s) 13, 1478
BciT130I CCWGG 7 cut(s) 773, 813, 840, 890, 1222, 1312, 1470
BciVI GTATCC 2 cut(s) 315, 1040
BcuI ACTAGT 2 cut(s) 197, 658
BfaI CTAG 2 cut(s) 198, 659
BfmI CTRYAG 2 cut(s) 1233, 1272
BfuI GTATCC 2 cut(s) 315, 1040
BglII AGATCT 1 cut(s) 703
BisI GCNGC 3 cut(s) 430, 495, 1545
BlsI GCNGC 3 cut(s) 431, 496, 1546
Bme1390I CCNGG 7 cut(s) 773, 813, 840, 890, 1222, 1312, 1470
Bme18I GGWCC 4 cut(s) 224, 481, 769, 1149
BmgBI CACGTC 1 cut(s) 1283
BmgT120I GGNCC 7 cut(s) 224, 481, 769, 807, 808, 1149, 1277
BmiI GGNNCC 4 cut(s) 225, 758, 809, 810
BmrFI CCNGG 7 cut(s) 773, 813, 840, 890, 1222, 1312, 1470
BmsI GCATC 6 cut(s) 247, 259, 447, 668, 844, 1556
BpmI CTGGAG 1 cut(s) 1290
Bpu10I CCTNAGC 2 cut(s) 408, 1157
BsaAI YACGTR 1 cut(s) 1438
BsaBI GATNNNNATC 1 cut(s) 261
BsaJI CCNNGG 5 cut(s) 158, 811, 838, 1220, 1468
BsaWI WCCGGW 1 cut(s) 226
Bsc4I CCNNNNNNNGG 2 cut(s) 490, 766
Bse1I ACTGG 2 cut(s) 105, 629
Bse3DI GCAATG 2 cut(s) 210, 245
Bse8I GATNNNNATC 1 cut(s) 261
BseBI CCWGG 7 cut(s) 773, 813, 840, 890, 1222, 1312, 1470
BseDI CCNNGG 5 cut(s) 158, 811, 838, 1220, 1468
BseGI GGATG 5 cut(s) 250, 683, 898, 936, 1495
BseJI GATNNNNATC 1 cut(s) 261
BseLI CCNNNNNNNGG 2 cut(s) 490, 766
BseMI GCAATG 2 cut(s) 210, 245
BseMII CTCAG 7 cut(s) 84, 105, 399, 461, 716, 1171, 1347
BseNI ACTGG 2 cut(s) 105, 629
BseRI GAGGAG 1 cut(s) 131
BseSI GKGCMC 1 cut(s) 811
BseXI GCAGC 3 cut(s) 441, 506, 1531
BseYI CCCAGC 1 cut(s) 1347
Bsh1236I CGCG 1 cut(s) 621
BshFI GGCC 6 cut(s) 235, 809, 1098, 1279, 1315, 1564
BsiHKAI GWGCWC 1 cut(s) 414
BsiSI CCGG 3 cut(s) 227, 760, 1013
BslFI GGGAC 3 cut(s) 1070, 1506, 1536
BslI CCNNNNNNNGG 2 cut(s) 490, 766
BsmFI GGGAC 3 cut(s) 1070, 1506, 1536
BsmI GAATGC 2 cut(s) 643, 1161
BsnI GGCC 6 cut(s) 235, 809, 1098, 1279, 1315, 1564
Bsp120I GGGCCC 1 cut(s) 807
Bsp1286I GDGCHC 2 cut(s) 414, 811
Bsp1407I TGTACA 1 cut(s) 895
Bsp143I GATC 5 cut(s) 283, 342, 703, 1110, 1477
BspACI CCGC 3 cut(s) 330, 619, 1301
BspANI GGCC 6 cut(s) 235, 809, 1098, 1279, 1315, 1564
BspCNI CTCAG 7 cut(s) 83, 106, 400, 460, 717, 1170, 1348
BspFNI CGCG 1 cut(s) 621
BspHI TCATGA 1 cut(s) 337
BspLI GGNNCC 4 cut(s) 225, 758, 809, 810
BspMAI CTGCAG 1 cut(s) 1276
BspPI GGATC 2 cut(s) 278, 1105
BsrDI GCAATG 2 cut(s) 210, 245
BsrGI TGTACA 1 cut(s) 895
BsrI ACTGG 2 cut(s) 105, 629
BssECI CCNNGG 5 cut(s) 158, 811, 838, 1220, 1468
BssMI GATC 5 cut(s) 283, 342, 703, 1110, 1477
BssT1I CCWWGG 1 cut(s) 158
Bst2UI CCWGG 7 cut(s) 773, 813, 840, 890, 1222, 1312, 1470
Bst4CI ACNGT 5 cut(s) 674, 927, 1237, 1384, 1419
Bst6I CTCTTC 5 cut(s) 705, 890, 1011, 1122, 1239
BstAUI TGTACA 1 cut(s) 895
BstBAI YACGTR 1 cut(s) 1438
BstC8I GCNNGC 3 cut(s) 683, 687, 1510
BstDEI CTNAG 9 cut(s) 70, 114, 408, 447, 725, 1157, 1175, 1356, 1513
BstF5I GGATG 5 cut(s) 250, 683, 898, 936, 1495
BstFNI CGCG 1 cut(s) 621
BstKTI GATC 5 cut(s) 286, 345, 706, 1113, 1480
BstMBI GATC 5 cut(s) 283, 342, 703, 1110, 1477
BstMWI GCNNNNNNNGC 2 cut(s) 435, 746
BstNI CCWGG 7 cut(s) 773, 813, 840, 890, 1222, 1312, 1470
BstSCI CCNGG 7 cut(s) 771, 811, 838, 888, 1220, 1310, 1468
BstSFI CTRYAG 2 cut(s) 1233, 1272
BstSLI GKGCMC 1 cut(s) 811
BstUI CGCG 1 cut(s) 621
BstV1I GCAGC 3 cut(s) 441, 506, 1531
BstX2I RGATCY 1 cut(s) 703
BstYI RGATCY 1 cut(s) 703
BsuI GTATCC 2 cut(s) 315, 1040
BsuRI GGCC 6 cut(s) 235, 809, 1098, 1279, 1315, 1564
BtgZI GCGATG 1 cut(s) 1139
BtrI CACGTC 1 cut(s) 1283
BtsCI GGATG 5 cut(s) 250, 683, 898, 936, 1495
BtsIMutI CAGTG 2 cut(s) 900, 932
Cac8I GCNNGC 3 cut(s) 683, 687, 1510
CaiI CAGNNNCTG 1 cut(s) 724
CciI TCATGA 1 cut(s) 337
Cfr13I GGNCC 7 cut(s) 224, 481, 769, 807, 808, 1149, 1277
Csp6I GTAC 3 cut(s) 715, 896, 1380
CviAII CATG 5 cut(s) 338, 490, 746, 869, 1263
CviQI GTAC 3 cut(s) 715, 896, 1380
DdeI CTNAG 9 cut(s) 70, 114, 408, 447, 725, 1157, 1175, 1356, 1513
DpnI GATC 5 cut(s) 285, 344, 705, 1112, 1479
DpnII GATC 5 cut(s) 283, 342, 703, 1110, 1477
DraI TTTAAA 2 cut(s) 580, 636
EaeI YGGCCR 1 cut(s) 1096
Eam1104I CTCTTC 5 cut(s) 705, 890, 1011, 1122, 1239
EarI CTCTTC 5 cut(s) 705, 890, 1011, 1122, 1239
Ecl136II GAGCTC 1 cut(s) 412
Eco130I CCWWGG 1 cut(s) 158
Eco147I AGGCCT 2 cut(s) 235, 1315
Eco24I GRGCYC 2 cut(s) 414, 811
Eco47I GGWCC 4 cut(s) 224, 481, 769, 1149
Eco53kI GAGCTC 1 cut(s) 412
EcoICRI GAGCTC 1 cut(s) 412
EcoO109I RGGNCCY 2 cut(s) 808, 1149
EcoRI GAATTC 2 cut(s) 349, 970
EcoRII CCWGG 7 cut(s) 771, 811, 838, 888, 1220, 1310, 1468
EcoT14I CCWWGG 1 cut(s) 158
EcoT38I GRGCYC 2 cut(s) 414, 811
ErhI CCWWGG 1 cut(s) 158
FaeI CATG 5 cut(s) 341, 493, 749, 872, 1266
FaqI GGGAC 3 cut(s) 1070, 1506, 1536
FatI CATG 5 cut(s) 337, 489, 745, 868, 1262
FauI CCCGC 1 cut(s) 323
FauNDI CATATG 1 cut(s) 1042
Fnu4HI GCNGC 3 cut(s) 430, 495, 1545
FokI GGATG 5 cut(s) 237, 690, 905, 943, 1482
FriOI GRGCYC 2 cut(s) 414, 811
Fsp4HI GCNGC 3 cut(s) 430, 495, 1545
FspBI CTAG 2 cut(s) 198, 659
GluI GCNGC 3 cut(s) 430, 495, 1545
GsaI CCCAGC 1 cut(s) 1351
GsuI CTGGAG 1 cut(s) 1290
HaeIII GGCC 6 cut(s) 235, 809, 1098, 1279, 1315, 1564
HapII CCGG 3 cut(s) 227, 760, 1013
Hin1II CATG 5 cut(s) 341, 493, 749, 872, 1266
HindIII AAGCTT 2 cut(s) 178, 1510
HinfI GANTC 4 cut(s) 242, 958, 1225, 1530
HpaII CCGG 3 cut(s) 227, 760, 1013
HphI GGTGA 3 cut(s) 185, 591, 979
Hpy166II GTNNAC 3 cut(s) 91, 898, 1229
Hpy188I TCNGA 4 cut(s) 130, 283, 295, 670
Hpy188III TCNNGA 8 cut(s) 239, 254, 338, 346, 407, 707, 1193, 1481
Hpy8I GTNNAC 3 cut(s) 91, 898, 1229
HpyAV CCTTC 4 cut(s) 5, 207, 1246, 1328
HpyCH4III ACNGT 5 cut(s) 674, 927, 1237, 1384, 1419
HpyCH4IV ACGT 3 cut(s) 940, 1282, 1437
HpyF10VI GCNNNNNNNGC 2 cut(s) 435, 746
HpyF3I CTNAG 9 cut(s) 70, 114, 408, 447, 725, 1157, 1175, 1356, 1513
HpySE526I ACGT 3 cut(s) 940, 1282, 1437
Hsp92II CATG 5 cut(s) 341, 493, 749, 872, 1266
Kzo9I GATC 5 cut(s) 283, 342, 703, 1110, 1477
LmnI GCTCC 2 cut(s) 417, 1171
Lsp1109I GCAGC 3 cut(s) 441, 506, 1531
LweI GCATC 6 cut(s) 247, 259, 447, 668, 844, 1556
MaeI CTAG 2 cut(s) 198, 659
MaeII ACGT 3 cut(s) 940, 1282, 1437
MalI GATC 5 cut(s) 285, 344, 705, 1112, 1479
MboI GATC 5 cut(s) 283, 342, 703, 1110, 1477
MfeI CAATTG 1 cut(s) 1428
MflI RGATCY 1 cut(s) 703
MhlI GDGCHC 2 cut(s) 414, 811
MlsI TGGCCA 1 cut(s) 1098
MluCI AATT 9 cut(s) 184, 289, 349, 485, 789, 970, 1391, 1428, 1501
MluNI TGGCCA 1 cut(s) 1098
MlyI GAGTC 2 cut(s) 1234, 1524
Mox20I TGGCCA 1 cut(s) 1098
MroXI GAANNNNTTC 1 cut(s) 909
MscI TGGCCA 1 cut(s) 1098
MseI TTAA 5 cut(s) 387, 453, 579, 635, 990
MslI CAYNNNNRTG 2 cut(s) 255, 873
Msp20I TGGCCA 1 cut(s) 1098
MspA1I CMGCKG 1 cut(s) 1544
MspI CCGG 3 cut(s) 227, 760, 1013
MspR9I CCNGG 7 cut(s) 773, 813, 840, 890, 1222, 1312, 1470
MunI CAATTG 1 cut(s) 1428
Mva1269I GAATGC 2 cut(s) 643, 1161
MvaI CCWGG 7 cut(s) 773, 813, 840, 890, 1222, 1312, 1470
MvnI CGCG 1 cut(s) 621
MwoI GCNNNNNNNGC 2 cut(s) 435, 746
NdeI CATATG 1 cut(s) 1042
NdeII GATC 5 cut(s) 283, 342, 703, 1110, 1477
NlaIII CATG 5 cut(s) 341, 493, 749, 872, 1266
NlaIV GGNNCC 4 cut(s) 225, 758, 809, 810
PagI TCATGA 1 cut(s) 337
PceI AGGCCT 2 cut(s) 235, 1315
PcsI WCGNNNNNNNCGW 1 cut(s) 1443
PctI GAATGC 2 cut(s) 643, 1161
PdmI GAANNNNTTC 1 cut(s) 909
PfeI GAWTC 2 cut(s) 242, 958
PflMI CCANNNNNTGG 1 cut(s) 490
PfoI TCCNGGA 2 cut(s) 771, 888
PkrI GCNGC 3 cut(s) 431, 496, 1546
PleI GAGTC 2 cut(s) 1233, 1524
PpsI GAGTC 2 cut(s) 1233, 1524
Ppu21I YACGTR 1 cut(s) 1438
PpuMI RGGWCCY 1 cut(s) 1149
Psp124BI GAGCTC 1 cut(s) 414
Psp5II RGGWCCY 1 cut(s) 1149
Psp6I CCWGG 7 cut(s) 771, 811, 838, 888, 1220, 1310, 1468
PspFI CCCAGC 1 cut(s) 1347
PspGI CCWGG 7 cut(s) 771, 811, 838, 888, 1220, 1310, 1468
PspN4I GGNNCC 4 cut(s) 225, 758, 809, 810
PspOMI GGGCCC 1 cut(s) 807
PspPI GGNCC 7 cut(s) 224, 481, 769, 807, 808, 1149, 1277
PspPPI RGGWCCY 1 cut(s) 1149
PstI CTGCAG 1 cut(s) 1276
PstNI CAGNNNCTG 1 cut(s) 724
PsuI RGATCY 1 cut(s) 703
PvuII CAGCTG 1 cut(s) 1544
RsaI GTAC 3 cut(s) 716, 897, 1381
RsaNI GTAC 3 cut(s) 715, 896, 1380
RseI CAYNNNNRTG 2 cut(s) 255, 873
SacI GAGCTC 1 cut(s) 414
SaqAI TTAA 5 cut(s) 387, 453, 579, 635, 990
SatI GCNGC 3 cut(s) 430, 495, 1545
Sau3AI GATC 5 cut(s) 283, 342, 703, 1110, 1477
Sau96I GGNCC 7 cut(s) 224, 481, 769, 807, 808, 1149, 1277
SchI GAGTC 2 cut(s) 1234, 1524
ScrFI CCNGG 7 cut(s) 773, 813, 840, 890, 1222, 1312, 1470
SduI GDGCHC 2 cut(s) 414, 811
SfaNI GCATC 6 cut(s) 247, 259, 447, 668, 844, 1556
SfcI CTRYAG 2 cut(s) 1233, 1272
SinI GGWCC 4 cut(s) 224, 481, 769, 1149
SmiMI CAYNNNNRTG 2 cut(s) 255, 873
SpeI ACTAGT 2 cut(s) 197, 658
Sse9I AATT 9 cut(s) 184, 289, 349, 485, 789, 970, 1391, 1428, 1501
SseBI AGGCCT 2 cut(s) 235, 1315
SsiI CCGC 3 cut(s) 330, 619, 1301
SspI AATATT 1 cut(s) 568
SspMI CTAG 2 cut(s) 198, 659
SstI GAGCTC 1 cut(s) 414
StuI AGGCCT 2 cut(s) 235, 1315
StyD4I CCNGG 7 cut(s) 771, 811, 838, 888, 1220, 1310, 1468
StyI CCWWGG 1 cut(s) 158
TaaI ACNGT 5 cut(s) 674, 927, 1237, 1384, 1419
TaiI ACGT 3 cut(s) 943, 1285, 1440
TaqI TCGA 4 cut(s) 106, 240, 353, 1239
TasI AATT 9 cut(s) 184, 289, 349, 485, 789, 970, 1391, 1428, 1501
TatI WGTACW 2 cut(s) 895, 1379
TfiI GAWTC 2 cut(s) 242, 958
Tru1I TTAA 5 cut(s) 387, 453, 579, 635, 990
Tru9I TTAA 5 cut(s) 387, 453, 579, 635, 990
TscAI CASTG 2 cut(s) 907, 932
TseI GCWGC 3 cut(s) 429, 494, 1544
TspDTI ATGAA 6 cut(s) 17, 53, 326, 478, 734, 1265
TspRI CASTG 2 cut(s) 907, 932
Van91I CCANNNNNTGG 1 cut(s) 490
VpaK11BI GGWCC 4 cut(s) 224, 481, 769, 1149
XapI RAATTY 5 cut(s) 184, 349, 789, 970, 1501
XcmI CCANNNNNNNNNTGG 1 cut(s) 856
XmnI GAANNNNTTC 1 cut(s) 909
XspI CTAG 2 cut(s) 198, 659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.