Rorug02G0509600

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003396
Physical Location & Seq
Reverse (-)
64032290 .. 64035241
2952 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0509600.1

Sequence Viewer

Length: 1182 bp
ATGTTGTTCCCATCTCTTCTCCTAAACCCTAAATTGAGGTTCCGTCCCCAGATGCGAAGTAAGAAACGCAGCAAAGATGAAGGGTCTCTTTTGATAGTGAAATTGAAGATGAAGGGTCTTCTCAAGAAATCCAAGGCACGCAAGCCTTTGGAGCTCGTCAAGCACACGCGCGACCTTCTCATCTTCTTCAATGGCAACATGGAAGTCCGCGAGCAGAAACGCAAAGACAAGACGGAAGAATTAACAAAATCAATGTTGGAGATAAGAACACTTCTGTATGGCGATGGTGAATCAGAGCCGAATAAAGACACTTGTGCACAGCTTACCCAGGAATTTTTCAAAGATGATACGTTCCGCCTTCTCATTGCTAGCCTTTCGAAGCTCAACCTTGGGACTCGTAAAAACGCGACACATATTGTCGCAAATTTGCAAAGGCAACAGGTTCAATCGCGATTGATTGCTTCTGAGTACTTGGAAAAGAATATTGATCTTATAGATATTCTGATACCTGGTTATGGAGACGGTGATATTGCTCTATCTTATGGTGCAATTTTAAGGGAATGCATTCGTCATCAGGTTGTTGCGAGGTACGTCCTGGAATCAGACCACATGAAGAAGTTCTTTGATTATATACAACTTCCAAATTTTGAAATAGCATCGGATGCTGCAGCCACTTTCAAGGAGCTTATGACCAGGCATAAGTCAACAGTTGCACAATTTTTGTCTAATAATTATGACTGGTTTTTCCAAGAATATAATTCGCAGTTGCTGGAGTCTCAGAATTACATCACCAAACGGCAGGCTATCAAGCTGTTAGGAAATATGTTACTTGATCGCTCAAACTCTGCTGTGATGGTTCGTTACGTGTGCTCTTTGGATAACATGAGAATCCTAATGAACCTTCTCAGGGATCCAAACAAGACAATCCAATTGGAGACCTTTCATGTCTTCAAGCTATTTGTTGCAAATGAAAATAAGCCTCCTCAGATTGTTAATGTACTAGTAACAAATAGGAGCAAGCTTCTCCGTTTCTTTGGCAACTTCACCATTGAAAAAGAGGATCAGCAGTTTGAAGCAGACAAAGTTGAACTTATGAAAGAGATTTCTATTCTTGAACCCAAGGATATTAAAGAATTTTCGTCAATCGTGAGCTTTAGAGAGAATTGTGAGATTCCATGTTGA

Protein Analysis

393

Amino Acids

45.81

Weight (kDa)

9.24

Isoelectric Point (pI)

36.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 41 - 371 3.3e-123 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 5 cut(s) 169, 171, 210, 407, 451
AciI CCGC 2 cut(s) 208, 355
AclWI GGATC 3 cut(s) 905, 918, 1068
AcsI RAATTY 4 cut(s) 332, 424, 643, 1133
AfaI GTAC 3 cut(s) 470, 590, 999
AfiI CCNNNNNNNGG 2 cut(s) 515, 907
AflIII ACRYGT 1 cut(s) 864
AhlI ACTAGT 1 cut(s) 1000
AjnI CCWGG 4 cut(s) 327, 508, 594, 692
AluBI AGCT 8 cut(s) 154, 322, 382, 685, 811, 955, 1021, 1152
AluI AGCT 8 cut(s) 154, 322, 382, 685, 811, 955, 1021, 1152
Alw21I GWGCWC 3 cut(s) 156, 319, 872
Alw26I GTCTC 4 cut(s) 90, 513, 780, 929
Alw44I GTGCAC 1 cut(s) 315
AlwI GGATC 3 cut(s) 905, 918, 1068
AlwNI CAGNNNCTG 1 cut(s) 769
ApaLI GTGCAC 1 cut(s) 315
ApeKI GCWGC 3 cut(s) 69, 665, 668
ApoI RAATTY 4 cut(s) 332, 424, 643, 1133
Asp700I GAANNNNTTC 2 cut(s) 564, 617
AspLEI GCGC 1 cut(s) 171
AsuHPI GGTGA 4 cut(s) 299, 536, 781, 1036
AsuII TTCGAA 1 cut(s) 377
AsuNHI GCTAGC 1 cut(s) 368
BaeGI GKGCMC 1 cut(s) 319
BamHI GGATCC 1 cut(s) 910
BanII GRGCYC 1 cut(s) 156
BbsI GAAGAC 2 cut(s) 110, 940
Bbv12I GWGCWC 3 cut(s) 156, 319, 872
BbvI GCAGC 3 cut(s) 81, 652, 680
BccI CCATC 3 cut(s) 19, 278, 847
BceAI ACGGC 1 cut(s) 812
BciT130I CCWGG 4 cut(s) 329, 510, 596, 694
BcoDI GTCTC 4 cut(s) 90, 513, 780, 929
BcuI ACTAGT 1 cut(s) 1000
BfaI CTAG 2 cut(s) 369, 1001
BfmI CTRYAG 1 cut(s) 666
BisI GCNGC 3 cut(s) 70, 666, 669
BlsI GCNGC 3 cut(s) 71, 667, 670
BmcAI AGTACT 1 cut(s) 470
Bme1390I CCNGG 4 cut(s) 329, 510, 596, 694
BmiI GGNNCC 2 cut(s) 41, 912
BmrFI CCNGG 4 cut(s) 329, 510, 596, 694
BmsI GCATC 3 cut(s) 42, 652, 665
BmtI GCTAGC 1 cut(s) 372
BpiI GAAGAC 2 cut(s) 110, 940
BpmI CTGGAG 1 cut(s) 791
Bpu14I TTCGAA 1 cut(s) 377
BpuEI CTTGAG 1 cut(s) 107
BsaAI YACGTR 1 cut(s) 865
BsaI GGTCTC 2 cut(s) 90, 929
BsaJI CCNNGG 4 cut(s) 132, 327, 388, 1119
Bsc4I CCNNNNNNNGG 2 cut(s) 515, 907
Bse1I ACTGG 1 cut(s) 743
Bse3DI GCAATG 1 cut(s) 363
BseBI CCWGG 4 cut(s) 329, 510, 596, 694
BseDI CCNNGG 4 cut(s) 132, 327, 388, 1119
BseGI GGATG 1 cut(s) 667
BseLI CCNNNNNNNGG 2 cut(s) 515, 907
BseMI GCAATG 1 cut(s) 363
BseMII CTCAG 4 cut(s) 456, 791, 919, 998
BseNI ACTGG 1 cut(s) 743
BseRI GAGGAG 1 cut(s) 972
BseSI GKGCMC 1 cut(s) 319
BseXI GCAGC 3 cut(s) 81, 652, 680
Bsh1236I CGCG 5 cut(s) 169, 171, 210, 407, 451
BsiHKAI GWGCWC 3 cut(s) 156, 319, 872
BslFI GGGAC 2 cut(s) 30, 406
BslI CCNNNNNNNGG 2 cut(s) 515, 907
BsmAI GTCTC 4 cut(s) 90, 513, 780, 929
BsmBI CGTCTC 1 cut(s) 513
BsmFI GGGAC 2 cut(s) 30, 406
BsmI GAATGC 2 cut(s) 564, 566
Bso31I GGTCTC 2 cut(s) 90, 929
Bsp119I TTCGAA 1 cut(s) 377
Bsp1286I GDGCHC 3 cut(s) 156, 319, 872
Bsp143I GATC 4 cut(s) 487, 832, 910, 1060
Bsp68I TCGCGA 1 cut(s) 451
BspACI CCGC 2 cut(s) 208, 355
BspCNI CTCAG 4 cut(s) 457, 790, 918, 997
BspFNI CGCG 5 cut(s) 169, 171, 210, 407, 451
BspLI GGNNCC 2 cut(s) 41, 912
BspMAI CTGCAG 1 cut(s) 670
BspOI GCTAGC 1 cut(s) 372
BspPI GGATC 3 cut(s) 905, 918, 1068
BspT104I TTCGAA 1 cut(s) 377
BspTNI GGTCTC 2 cut(s) 90, 929
BsrDI GCAATG 1 cut(s) 363
BsrI ACTGG 1 cut(s) 743
BssECI CCNNGG 4 cut(s) 132, 327, 388, 1119
BssMI GATC 4 cut(s) 487, 832, 910, 1060
BssT1I CCWWGG 3 cut(s) 132, 388, 1119
Bst2UI CCWGG 4 cut(s) 329, 510, 596, 694
Bst4CI ACNGT 2 cut(s) 524, 709
Bst6I CTCTTC 1 cut(s) 21
BstAPI GCANNNNNTGC 1 cut(s) 662
BstBAI YACGTR 1 cut(s) 865
BstBI TTCGAA 1 cut(s) 377
BstC8I GCNNGC 6 cut(s) 139, 143, 212, 370, 801, 1019
BstDEI CTNAG 4 cut(s) 465, 777, 905, 984
BstF5I GGATG 1 cut(s) 667
BstFNI CGCG 5 cut(s) 169, 171, 210, 407, 451
BstHHI GCGC 1 cut(s) 171
BstKTI GATC 4 cut(s) 490, 835, 913, 1063
BstMAI GTCTC 4 cut(s) 90, 513, 780, 929
BstMBI GATC 4 cut(s) 487, 832, 910, 1060
BstMWI GCNNNNNNNGC 3 cut(s) 151, 160, 662
BstNI CCWGG 4 cut(s) 329, 510, 596, 694
BstSCI CCNGG 4 cut(s) 327, 508, 594, 692
BstSFI CTRYAG 1 cut(s) 666
BstSLI GKGCMC 1 cut(s) 319
BstUI CGCG 5 cut(s) 169, 171, 210, 407, 451
BstV1I GCAGC 3 cut(s) 81, 652, 680
BstV2I GAAGAC 2 cut(s) 110, 940
BstX2I RGATCY 1 cut(s) 910
BstYI RGATCY 1 cut(s) 910
BtgZI GCGATG 1 cut(s) 297
BtsCI GGATG 1 cut(s) 667
BtuMI TCGCGA 1 cut(s) 451
Cac8I GCNNGC 6 cut(s) 139, 143, 212, 370, 801, 1019
CaiI CAGNNNCTG 1 cut(s) 769
CfoI GCGC 1 cut(s) 171
CsiI ACCWGGT 1 cut(s) 508
Csp6I GTAC 3 cut(s) 469, 589, 998
CviAII CATG 5 cut(s) 199, 610, 883, 944, 1176
CviQI GTAC 3 cut(s) 469, 589, 998
DdeI CTNAG 4 cut(s) 465, 777, 905, 984
DpnI GATC 4 cut(s) 489, 834, 912, 1062
DpnII GATC 4 cut(s) 487, 832, 910, 1060
Eam1104I CTCTTC 1 cut(s) 21
EarI CTCTTC 1 cut(s) 21
EciI GGCGGA 1 cut(s) 344
Ecl136II GAGCTC 1 cut(s) 154
Eco130I CCWWGG 3 cut(s) 132, 388, 1119
Eco24I GRGCYC 1 cut(s) 156
Eco31I GGTCTC 2 cut(s) 90, 929
Eco53kI GAGCTC 1 cut(s) 154
EcoICRI GAGCTC 1 cut(s) 154
EcoRII CCWGG 4 cut(s) 327, 508, 594, 692
EcoT14I CCWWGG 3 cut(s) 132, 388, 1119
EcoT22I ATGCAT 1 cut(s) 566
EcoT38I GRGCYC 1 cut(s) 156
ErhI CCWWGG 3 cut(s) 132, 388, 1119
Esp3I CGTCTC 1 cut(s) 513
FaeI CATG 5 cut(s) 202, 613, 886, 947, 1179
FalI AAGNNNNNCTT 6 cut(s) 72, 104, 605, 637, 1074, 1106
FaqI GGGAC 2 cut(s) 30, 406
FatI CATG 5 cut(s) 198, 609, 882, 943, 1175
Fnu4HI GCNGC 3 cut(s) 70, 666, 669
FokI GGATG 1 cut(s) 674
FriOI GRGCYC 1 cut(s) 156
Fsp4HI GCNGC 3 cut(s) 70, 666, 669
FspBI CTAG 2 cut(s) 369, 1001
GlaI GCGC 1 cut(s) 170
GluI GCNGC 3 cut(s) 70, 666, 669
GsuI CTGGAG 1 cut(s) 791
HhaI GCGC 1 cut(s) 171
Hin1II CATG 5 cut(s) 202, 613, 886, 947, 1179
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HincII GTYRAC 1 cut(s) 705
HindII GTYRAC 1 cut(s) 705
HindIII AAGCTT 1 cut(s) 1019
HinfI GANTC 6 cut(s) 290, 394, 599, 773, 888, 1171
HphI GGTGA 4 cut(s) 299, 536, 781, 1036
Hpy166II GTNNAC 2 cut(s) 317, 705
Hpy188I TCNGA 7 cut(s) 295, 466, 504, 604, 661, 780, 987
Hpy188III TCNNGA 4 cut(s) 124, 450, 1112, 1147
Hpy8I GTNNAC 2 cut(s) 317, 705
HpyAV CCTTC 5 cut(s) 74, 106, 185, 368, 911
HpyCH4III ACNGT 2 cut(s) 524, 709
HpyCH4IV ACGT 3 cut(s) 350, 591, 864
HpyCH4V TGCA 7 cut(s) 317, 430, 548, 564, 668, 713, 965
HpyF10VI GCNNNNNNNGC 3 cut(s) 151, 160, 662
HpyF3I CTNAG 4 cut(s) 465, 777, 905, 984
HpySE526I ACGT 3 cut(s) 350, 591, 864
Hsp92II CATG 5 cut(s) 202, 613, 886, 947, 1179
HspAI GCGC 1 cut(s) 169
Kzo9I GATC 4 cut(s) 487, 832, 910, 1060
LmnI GCTCC 3 cut(s) 151, 682, 1014
Lsp1109I GCAGC 3 cut(s) 81, 652, 680
LweI GCATC 3 cut(s) 42, 652, 665
MabI ACCWGGT 1 cut(s) 508
MaeI CTAG 2 cut(s) 369, 1001
MaeII ACGT 3 cut(s) 350, 591, 864
MaeIII GTNAC 3 cut(s) 825, 860, 1003
MalI GATC 4 cut(s) 489, 834, 912, 1062
MboI GATC 4 cut(s) 487, 832, 910, 1060
MboII GAAGA 8 cut(s) 8, 110, 118, 175, 178, 248, 625, 940
MfeI CAATTG 1 cut(s) 929
MflI RGATCY 1 cut(s) 910
MhlI GDGCHC 3 cut(s) 156, 319, 872
MlyI GAGTC 2 cut(s) 388, 782
MmeI TCCRAC 1 cut(s) 237
MnlI CCTC 5 cut(s) 30, 579, 990, 993, 1051
Mph1103I ATGCAT 1 cut(s) 566
MroXI GAANNNNTTC 2 cut(s) 564, 617
MseI TTAA 4 cut(s) 242, 554, 993, 1128
MspR9I CCNGG 4 cut(s) 329, 510, 596, 694
MunI CAATTG 1 cut(s) 929
Mva1269I GAATGC 2 cut(s) 564, 566
MvaI CCWGG 4 cut(s) 329, 510, 596, 694
MvnI CGCG 5 cut(s) 169, 171, 210, 407, 451
MwoI GCNNNNNNNGC 3 cut(s) 151, 160, 662
NdeII GATC 4 cut(s) 487, 832, 910, 1060
NheI GCTAGC 1 cut(s) 368
NlaIII CATG 5 cut(s) 202, 613, 886, 947, 1179
NlaIV GGNNCC 2 cut(s) 41, 912
NruI TCGCGA 1 cut(s) 451
NsiI ATGCAT 1 cut(s) 566
NspV TTCGAA 1 cut(s) 377
PctI GAATGC 2 cut(s) 564, 566
PdmI GAANNNNTTC 2 cut(s) 564, 617
PfeI GAWTC 4 cut(s) 290, 599, 888, 1171
PfoI TCCNGGA 1 cut(s) 594
PkrI GCNGC 3 cut(s) 71, 667, 670
PleI GAGTC 2 cut(s) 388, 781
PpsI GAGTC 2 cut(s) 388, 781
Ppu21I YACGTR 1 cut(s) 865
Psp124BI GAGCTC 1 cut(s) 156
Psp6I CCWGG 4 cut(s) 327, 508, 594, 692
PspGI CCWGG 4 cut(s) 327, 508, 594, 692
PspN4I GGNNCC 2 cut(s) 41, 912
PstI CTGCAG 1 cut(s) 670
PstNI CAGNNNCTG 1 cut(s) 769
PsuI RGATCY 1 cut(s) 910
RruI TCGCGA 1 cut(s) 451
RsaI GTAC 3 cut(s) 470, 590, 999
RsaNI GTAC 3 cut(s) 469, 589, 998
SacI GAGCTC 1 cut(s) 156
SaqAI TTAA 4 cut(s) 242, 554, 993, 1128
SatI GCNGC 3 cut(s) 70, 666, 669
Sau3AI GATC 4 cut(s) 487, 832, 910, 1060
ScaI AGTACT 1 cut(s) 470
SchI GAGTC 2 cut(s) 388, 782
ScrFI CCNGG 4 cut(s) 329, 510, 596, 694
SduI GDGCHC 3 cut(s) 156, 319, 872
SexAI ACCWGGT 1 cut(s) 508
SfaNI GCATC 3 cut(s) 42, 652, 665
SfcI CTRYAG 1 cut(s) 666
SfuI TTCGAA 1 cut(s) 377
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
SpeI ACTAGT 1 cut(s) 1000
SsiI CCGC 2 cut(s) 208, 355
SspI AATATT 1 cut(s) 484
SspMI CTAG 2 cut(s) 369, 1001
SstI GAGCTC 1 cut(s) 156
StyD4I CCNGG 4 cut(s) 327, 508, 594, 692
StyI CCWWGG 3 cut(s) 132, 388, 1119
TaaI ACNGT 2 cut(s) 524, 709
TaiI ACGT 3 cut(s) 353, 594, 867
TaqI TCGA 1 cut(s) 377
TatI WGTACW 2 cut(s) 468, 997
TfiI GAWTC 4 cut(s) 290, 599, 888, 1171
Tru1I TTAA 4 cut(s) 242, 554, 993, 1128
Tru9I TTAA 4 cut(s) 242, 554, 993, 1128
TseI GCWGC 3 cut(s) 69, 665, 668
TspDTI ATGAA 7 cut(s) 93, 125, 626, 911, 932, 984, 1109
TspGWI ACGGA 3 cut(s) 32, 248, 1016
VneI GTGCAC 1 cut(s) 315
XapI RAATTY 4 cut(s) 332, 424, 643, 1133
XmnI GAANNNNTTC 2 cut(s) 564, 617
XspI CTAG 2 cut(s) 369, 1001
ZrmI AGTACT 1 cut(s) 470
Zsp2I ATGCAT 1 cut(s) 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.