RchiOBHm_Chr6g0258841

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003396
Physical Location & Seq
Forward (+)
14196459 .. 14197192
734 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23209

Sequence Viewer

Length: 600 bp
ATGCCTTTATACTTATTGTTGGTGCCACTCAAGGATGTACACACTAAACTCATTCGGCACAAAAGACAGCGGATGATAAAAAAACATGGGATTGTCAAGGGGAATCTTACGGTGAATTCTGCAAACCAACGACTAAAAGGTTCTTGCCCACTAATGCCAGAAGAGATTGGTATTATTCTTTGTGCATATGGATACTTGTGGGACACAATAATATATGTTTCTGGGGGCGAAATTTTTGGTGGCCAAAGGAAATTGATCCCTCTTCTCGCAATGTTTAAAAATGTAGTTGATGGGACCTCCCTCAGCATTTTCTGGGAGCTTAGTAGGATTTATGGTCATGAGGCTAACCTCTTTGAAAGTAATCCCAGGAGTCCACCTACAGTTGAGGAAGAGAAGAAGGTTGAAGCATGGAAAACTGTAGGGCCACGTCCTCTTCCACTTCCACTTCCACCGCCTTCAGCCAGGCCCAAATCATATAACATTGAAGGTTGGTGGGGATGGGTCGCTGAGAGTGATAATAAGCCTGATAGTACAGCTATGGAATTGAGGACAAACGCTCATAAACTACTGTGGGGTGCAATTGATTACGTGATGCGTTGA

Protein Analysis

199

Amino Acids

22.6

Weight (kDa)

9.41

Isoelectric Point (pI)

44.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 12 - 115 3.4e-13 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 22
AciI CCGC 2 cut(s) 70, 452
AclWI GGATC 1 cut(s) 250
AcoI YGGCCR 1 cut(s) 241
AcsI RAATTY 2 cut(s) 115, 231
AcuI CTGAAG 1 cut(s) 441
AfaI GTAC 2 cut(s) 39, 532
AgsI TTSAA 3 cut(s) 356, 404, 485
AjiI CACGTC 1 cut(s) 428
AjnI CCWGG 2 cut(s) 365, 461
AluBI AGCT 2 cut(s) 319, 536
AluI AGCT 2 cut(s) 319, 536
AlwI GGATC 1 cut(s) 250
AoxI GGCC 3 cut(s) 241, 422, 464
ApoI RAATTY 2 cut(s) 115, 231
AspS9I GGNCC 3 cut(s) 294, 422, 465
AsuHPI GGTGA 1 cut(s) 124
AvaII GGWCC 1 cut(s) 294
BalI TGGCCA 1 cut(s) 243
BanI GGYRCC 1 cut(s) 22
BbvCI CCTCAGC 1 cut(s) 302
BccI CCATC 2 cut(s) 284, 492
BciT130I CCWGG 2 cut(s) 367, 463
BciVI GTATCC 1 cut(s) 185
BfmI CTRYAG 2 cut(s) 378, 417
BfuI GTATCC 1 cut(s) 185
Bme1390I CCNGG 2 cut(s) 367, 463
Bme18I GGWCC 1 cut(s) 294
BmgBI CACGTC 1 cut(s) 428
BmgT120I GGNCC 3 cut(s) 294, 422, 465
BmiI GGNNCC 2 cut(s) 24, 295
BmrFI CCNGG 2 cut(s) 367, 463
BmsI GCATC 1 cut(s) 582
Bpu10I CCTNAGC 1 cut(s) 302
BpuEI CTTGAG 1 cut(s) 14
BsaAI YACGTR 1 cut(s) 589
BsaJI CCNNGG 1 cut(s) 365
Bse3DI GCAATG 1 cut(s) 276
BseBI CCWGG 2 cut(s) 367, 463
BseDI CCNNGG 1 cut(s) 365
BseGI GGATG 3 cut(s) 40, 78, 503
BseMI GCAATG 1 cut(s) 276
BseMII CTCAG 2 cut(s) 316, 498
BshFI GGCC 3 cut(s) 243, 424, 466
BshNI GGYRCC 1 cut(s) 22
BslFI GGGAC 2 cut(s) 215, 307
BsmFI GGGAC 2 cut(s) 215, 307
BsnI GGCC 3 cut(s) 243, 424, 466
Bsp1407I TGTACA 1 cut(s) 37
Bsp143I GATC 1 cut(s) 255
BspACI CCGC 2 cut(s) 70, 452
BspANI GGCC 3 cut(s) 243, 424, 466
BspCNI CTCAG 2 cut(s) 315, 499
BspHI TCATGA 1 cut(s) 337
BspLI GGNNCC 2 cut(s) 24, 295
BspPI GGATC 1 cut(s) 250
BspT107I GGYRCC 1 cut(s) 22
BsrDI GCAATG 1 cut(s) 276
BsrGI TGTACA 1 cut(s) 37
BssECI CCNNGG 1 cut(s) 365
BssMI GATC 1 cut(s) 255
Bst2UI CCWGG 2 cut(s) 367, 463
Bst4CI ACNGT 4 cut(s) 112, 382, 418, 570
Bst6I CTCTTC 4 cut(s) 156, 267, 384, 438
BstAUI TGTACA 1 cut(s) 37
BstBAI YACGTR 1 cut(s) 589
BstDEI CTNAG 3 cut(s) 302, 320, 507
BstF5I GGATG 3 cut(s) 40, 78, 503
BstKTI GATC 1 cut(s) 258
BstMBI GATC 1 cut(s) 255
BstNI CCWGG 2 cut(s) 367, 463
BstSCI CCNGG 2 cut(s) 365, 461
BstSFI CTRYAG 2 cut(s) 378, 417
BsuI GTATCC 1 cut(s) 185
BsuRI GGCC 3 cut(s) 243, 424, 466
BtrI CACGTC 1 cut(s) 428
BtsCI GGATG 3 cut(s) 40, 78, 503
CciI TCATGA 1 cut(s) 337
Cfr13I GGNCC 3 cut(s) 294, 422, 465
Csp6I GTAC 2 cut(s) 38, 531
CviAII CATG 3 cut(s) 86, 338, 408
CviJI RGCY 8 cut(s) 243, 319, 344, 424, 461, 466, 523, 536
CviKI_1 RGCY 8 cut(s) 243, 319, 344, 424, 461, 466, 523, 536
CviQI GTAC 2 cut(s) 38, 531
DdeI CTNAG 3 cut(s) 302, 320, 507
DpnI GATC 1 cut(s) 257
DpnII GATC 1 cut(s) 255
DraI TTTAAA 1 cut(s) 277
EaeI YGGCCR 1 cut(s) 241
Eam1104I CTCTTC 4 cut(s) 156, 267, 384, 438
EarI CTCTTC 4 cut(s) 156, 267, 384, 438
Eco47I GGWCC 1 cut(s) 294
Eco57I CTGAAG 1 cut(s) 441
EcoO109I RGGNCCY 1 cut(s) 294
EcoRI GAATTC 1 cut(s) 115
EcoRII CCWGG 2 cut(s) 365, 461
FaeI CATG 3 cut(s) 89, 341, 411
FaqI GGGAC 2 cut(s) 215, 307
FatI CATG 3 cut(s) 85, 337, 407
FauNDI CATATG 1 cut(s) 187
FokI GGATG 3 cut(s) 47, 85, 510
HaeIII GGCC 3 cut(s) 243, 424, 466
Hin1II CATG 3 cut(s) 89, 341, 411
HinfI GANTC 2 cut(s) 103, 370
HphI GGTGA 1 cut(s) 124
Hpy166II GTNNAC 2 cut(s) 40, 374
Hpy188III TCNNGA 1 cut(s) 338
Hpy8I GTNNAC 2 cut(s) 40, 374
HpyAV CCTTC 3 cut(s) 391, 465, 479
HpyCH4III ACNGT 4 cut(s) 112, 382, 418, 570
HpyCH4IV ACGT 2 cut(s) 427, 588
HpyCH4V TGCA 3 cut(s) 122, 185, 578
HpyF3I CTNAG 3 cut(s) 302, 320, 507
HpySE526I ACGT 2 cut(s) 427, 588
Hsp92II CATG 3 cut(s) 89, 341, 411
Kzo9I GATC 1 cut(s) 255
LmnI GCTCC 1 cut(s) 316
LpnPI CCDG 8 cut(s) 171, 207, 298, 352, 379, 448, 475, 537
LweI GCATC 1 cut(s) 582
MaeII ACGT 2 cut(s) 427, 588
MalI GATC 1 cut(s) 257
MboI GATC 1 cut(s) 255
MboII GAAGA 5 cut(s) 173, 254, 401, 406, 425
MfeI CAATTG 1 cut(s) 579
MlsI TGGCCA 1 cut(s) 243
MluCI AATT 5 cut(s) 115, 231, 251, 542, 579
MluNI TGGCCA 1 cut(s) 243
MlyI GAGTC 1 cut(s) 379
MnlI CCTC 8 cut(s) 270, 307, 311, 334, 359, 379, 441, 540
Mox20I TGGCCA 1 cut(s) 243
MscI TGGCCA 1 cut(s) 243
MseI TTAA 1 cut(s) 276
Msp20I TGGCCA 1 cut(s) 243
MspA1I CMGCKG 1 cut(s) 70
MspR9I CCNGG 2 cut(s) 367, 463
MunI CAATTG 1 cut(s) 579
MvaI CCWGG 2 cut(s) 367, 463
NdeI CATATG 1 cut(s) 187
NdeII GATC 1 cut(s) 255
NlaIII CATG 3 cut(s) 89, 341, 411
NlaIV GGNNCC 2 cut(s) 24, 295
PagI TCATGA 1 cut(s) 337
PfeI GAWTC 1 cut(s) 103
PleI GAGTC 1 cut(s) 378
PpsI GAGTC 1 cut(s) 378
Ppu21I YACGTR 1 cut(s) 589
PpuMI RGGWCCY 1 cut(s) 294
Psp5II RGGWCCY 1 cut(s) 294
Psp6I CCWGG 2 cut(s) 365, 461
PspGI CCWGG 2 cut(s) 365, 461
PspN4I GGNNCC 2 cut(s) 24, 295
PspPI GGNCC 3 cut(s) 294, 422, 465
PspPPI RGGWCCY 1 cut(s) 294
RsaI GTAC 2 cut(s) 39, 532
RsaNI GTAC 2 cut(s) 38, 531
SaqAI TTAA 1 cut(s) 276
Sau3AI GATC 1 cut(s) 255
Sau96I GGNCC 3 cut(s) 294, 422, 465
SchI GAGTC 1 cut(s) 379
ScrFI CCNGG 2 cut(s) 367, 463
SfaNI GCATC 1 cut(s) 582
SfcI CTRYAG 2 cut(s) 378, 417
SinI GGWCC 1 cut(s) 294
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 5 cut(s) 115, 231, 251, 542, 579
SsiI CCGC 2 cut(s) 70, 452
StyD4I CCNGG 2 cut(s) 365, 461
TaaI ACNGT 4 cut(s) 112, 382, 418, 570
TaiI ACGT 2 cut(s) 430, 591
TasI AATT 5 cut(s) 115, 231, 251, 542, 579
TatI WGTACW 2 cut(s) 37, 530
TfiI GAWTC 1 cut(s) 103
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
VpaK11BI GGWCC 1 cut(s) 294
XapI RAATTY 2 cut(s) 115, 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.